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The transferrin iron acquisition system of Neisseria consists of two dissimilar proteins, transferrin binding protein A and B (TbpA and TbpB). TbpA and TbpB both specifically and independently bind human transferrin (Tf). TbpA is a TonB-dependent transporter, expression of which is necessary for Tf iron acquisition. In contrast, the lipoprotein TbpB is not necessary for iron internalization; however it makes this process more efficient. The role of TbpB in the transferrin iron acquisition system has not been completely elucidated. It has been suggested that TbpB is entirely surface exposed and tethered to the outer membrane by its lipid moiety. We inserted the hemagluttinin antigen (HA) epitope into TbpB in an effort to examine surface accessible and functional domains of the lipoprotein. We determined that TbpB was entirely surface exposed from just beyond the mature N-terminus. It was previously reported that the N- and C-terminus of TbpB independently bind Tf. HA epitope analysis defined both the N-terminal and C-terminal binding domains. TbpB was previously reported to play an important role in the release of Tf from the receptor. We established that TbpB exhibited a biphasic dissociation pattern; a C-terminal rapid release followed by a slower N-terminal release. These results suggested that the C-terminus plays a role in ligand turnover of the wild-type receptor. Little is known about the transport of TbpB to the outer membrane. In an attempt to identify the signals/mechanisms required for TbpB localization, the signal sequence of the protein was altered. In the absence of lipid modification, TbpB remained associated with the cell, localized to the periplasm. We also noted that internal cysteine residues were not critical for TbpB localization. Our results suggested that TbpB was transported by a lipoprotein-specific mechanism. Additionally, we demonstrated the major outer membrane secretin, PilQ, was not necessary for proper localization of TbpB. The mechanism responsible for this process remains elusive. This body of work represents the first comprehensive study of TbpB topology and function, utilizing the lipoprotein expressed in its native membrane. These results may translate to other, similar lipoprotein receptors of the pathogenic Neisseria, helping to shed light on these poorly understood proteins.

FbpA is the periplasmic binding protein of the transferrin and lactoferrin-iron transport systems. FbpA is conserved among neisserial species and is required for Neisseria gonorrhoeae to sustain growth on transferrin and lactoferrin. The identification of other putative TonB-dependent outer membrane transporters suggests that gonococci may employ other uncharacterized iron uptake systems that do not require FbpA. Previous work in our lab demonstrated that gonococcal strain FA19 utilizes iron from a number of xenosiderophores of the catecholate and hydroxamate classes. In this study we created conditional FbpA mutants to evaluate whether FbpA plays a role in the ability of gonococci to utilize iron from xenosiderophores. Strain FA19 was able to acquire iron from the xenosiderophores enterobactin and salmochelin in an FbpA-dependent and TonB-independent manner. We were also able to detect an extracellular population of FbpA indicating that FbpA may play a novel role in the internalization of iron in the absence of a dedicated transporter.

Avaliar a associação entre as infecções genito-urinárias, o comprimento do colo uterino e a prematuridade é importante para determinar quais podem ser os fatores preditivos para o parto pré-termo. Foi realizado estudo tipo coorte de conveniência, prospectivo, avaliando 1370 gestantes na cidade de Ribeirão Preto, com idade gestacional entre 20 e 25 semanas. Aplicou-se questionário sócio demográfico com história reprodutiva para a identificação do histórico obstétrico, idade materna, paridade, tabagismo e antecedente de parto pré-termo. Foi realizada ultrassonografia endovaginal para a avaliação do comprimento do colo uterino segundo as diretrizes da Fetal Medicine Foundation (FMF). Foram coletadas amostras de urina e conteúdo vaginal para avaliar a presença de infecção urinária e vaginose bacteriana respectivamente. A associação entre infecções, comprimento do colo uterino e parto pré-termo (PPT) foi realizada mediante teste não paramétrico e o cálculo do Risco Relativo das diferentes variáveis, por meio do ajuste de modelos log-binomiais. Das 1370 mulheres grávidas avaliadas, 132(9,63%) cursaram com parto pré-termo (<37 semanas), sendo que 19 (14,4%) dos partos pré-termo ocorreram em mulheres com colo <= 2,5 cm. O estudo microbiológico determinou que no grupo das mulheres que cursaram com parto pré-termo, 15 apresentaram ITU, 19 apresentaram vaginose bacteriana (VB) e uma apresentou ITU e VB. Avaliando as 75 pacientes com PPT espontâneos, 10 apresentaram ITU e 14, VB. Após a análise destes dados, foi possível concluir que o colo uterino curto entre 20 e 25 semanas de gestação está associado ao PPT e que ITU e VB rastreadas nesta idade não se associaram ao encurtamento do colo nem ao PPT. No entanto a ITU, mesmo assintomática apresentou relação com o PPT espontâneo. / To evaluate the association between the genito-urinary infections, cervical length and preterm birth is important to determine which can be predictive factors for preterm birth. It was conducted cohort study of convenience, prospective, evaluating 1370 pregnant women in the city of Ribeirão Preto, with gestational age between 20 and 25 weeks. Applied socio-demographic questionnaire with reproductive history to identify the obstetric history, maternal age, parity, smoking and preterm birth (PTB) history. Transvaginal ultrasonography was performed for evaluation of cervical length in the guidelines of the Fetal Medicine Foundation (FMF). Urine and vaginal discharge samples were collected to evaluate the presence of urinary tract infection (UTI) and bacterial vaginosis (BV) respectively. The association between infections, cervical length and preterm delivery was performed using non-parametric test and calculate the relative risk of different variables, by adjusting log-binomial model. Of the 1370 evaluated pregnant women, 132 (9.63%) presenting with preterm delivery (<37 weeks), and 19 (14.4%) of preterm deliveries occurred in women with cervix <=2.5 cm. The microbiological study found that the group of women presenting with preterm birth(PTB), 15 had UTI, 19 had BV and one presented UTI and VB. Evaluating 75 patients with spontaneous PTB, 10 had UTI and 14, BV. After the analysis of these data, it was concluded that the short cervix between 20 and 25 weeks of gestation is associated with the PTB and UTI and VB screened at this age not associated to the shortening of the cervix or the PTB. However, the UTI even asymptomatic were related to the spontaneous PTB.

Avaliar a associação entre as infecções genito-urinárias, o comprimento do colo uterino e a prematuridade é importante para determinar quais podem ser os fatores preditivos para o parto pré-termo. Foi realizado estudo tipo coorte de conveniência, prospectivo, avaliando 1370 gestantes na cidade de Ribeirão Preto, com idade gestacional entre 20 e 25 semanas. Aplicou-se questionário sócio demográfico com história reprodutiva para a identificação do histórico obstétrico, idade materna, paridade, tabagismo e antecedente de parto pré-termo. Foi realizada ultrassonografia endovaginal para a avaliação do comprimento do colo uterino segundo as diretrizes da Fetal Medicine Foundation (FMF). Foram coletadas amostras de urina e conteúdo vaginal para avaliar a presença de infecção urinária e vaginose bacteriana respectivamente. A associação entre infecções, comprimento do colo uterino e parto pré-termo (PPT) foi realizada mediante teste não paramétrico e o cálculo do Risco Relativo das diferentes variáveis, por meio do ajuste de modelos log-binomiais. Das 1370 mulheres grávidas avaliadas, 132(9,63%) cursaram com parto pré-termo (<37 semanas), sendo que 19 (14,4%) dos partos pré-termo ocorreram em mulheres com colo <= 2,5 cm. O estudo microbiológico determinou que no grupo das mulheres que cursaram com parto pré-termo, 15 apresentaram ITU, 19 apresentaram vaginose bacteriana (VB) e uma apresentou ITU e VB. Avaliando as 75 pacientes com PPT espontâneos, 10 apresentaram ITU e 14, VB. Após a análise destes dados, foi possível concluir que o colo uterino curto entre 20 e 25 semanas de gestação está associado ao PPT e que ITU e VB rastreadas nesta idade não se associaram ao encurtamento do colo nem ao PPT. No entanto a ITU, mesmo assintomática apresentou relação com o PPT espontâneo. / To evaluate the association between the genito-urinary infections, cervical length and preterm birth is important to determine which can be predictive factors for preterm birth. It was conducted cohort study of convenience, prospective, evaluating 1370 pregnant women in the city of Ribeirão Preto, with gestational age between 20 and 25 weeks. Applied socio-demographic questionnaire with reproductive history to identify the obstetric history, maternal age, parity, smoking and preterm birth (PTB) history. Transvaginal ultrasonography was performed for evaluation of cervical length in the guidelines of the Fetal Medicine Foundation (FMF). Urine and vaginal discharge samples were collected to evaluate the presence of urinary tract infection (UTI) and bacterial vaginosis (BV) respectively. The association between infections, cervical length and preterm delivery was performed using non-parametric test and calculate the relative risk of different variables, by adjusting log-binomial model. Of the 1370 evaluated pregnant women, 132 (9.63%) presenting with preterm delivery (<37 weeks), and 19 (14.4%) of preterm deliveries occurred in women with cervix <=2.5 cm. The microbiological study found that the group of women presenting with preterm birth(PTB), 15 had UTI, 19 had BV and one presented UTI and VB. Evaluating 75 patients with spontaneous PTB, 10 had UTI and 14, BV. After the analysis of these data, it was concluded that the short cervix between 20 and 25 weeks of gestation is associated with the PTB and UTI and VB screened at this age not associated to the shortening of the cervix or the PTB. However, the UTI even asymptomatic were related to the spontaneous PTB.

Conselho Nacional de Desenvolvimento Científico e Tecnológico / We herein report an evaluation of the attenuation and protection conferred by genital vaccination of heifers with a bovine herpesvirus type 1 strain (BoHV-1) defective in the glycoprotein E (SV265gE-). A group of six seronegative heifers was vaccinated with SV265gE- in the submucosa of the vulva (group IV; 106.9TCID50); four heifers were vaccinated intramuscularly (group IM; 107.6TCID50) and four heifers remained as nonvaccinated controls. Heifers vaccinated IV developed mild and transient local edema and hyperemia and shed low amounts of virus for a few days after vaccination, yet a sentinel heifer maintained in close contact did not seroconvert. Attempts to reactivate the vaccine virus in two IV vaccinated heifers by intravenous administration of dexamethasone (0.5 mg.kg-1) at day 65 post-vaccination (pv) failed since no virus shedding, recrudescence of genital signs or seroconversion were observed. At day 65 pv, all vaccinated and control heifers were challenged by genital inoculation of a highly virulent BoHV-1.2 isolate (SV-56/90, 107.4TCID50/animal). After challenge, virus shedding was detected in genital secretions of control animals for 8.2 days (8 9 days); in the IM group for 6.2 days (5 8 days) and during 5.2 days (5 6 days) in the IV group. Control non-vaccinated heifers developed moderate (2/4) or severe (2/4) vulvovaginitis lasting 9 to 14 days ( 11.2 days). The disease was characterized by vulvar edema, vulvovestibular congestion, small vesicles progressing to coalescence and erosions, fibrinonecrotic plaques and fibrinopurulent exsudate. IM vaccinated heifers developed mild (1/3) or moderate (3/4) genital lesions, lasting 10 to 13 days ( 11.5 days); and IV vaccinated heifers developed mild and transient (3/4) or mild to moderate genital signs (1/4), lasting 4 to 8 days ( 5.5 days). Clinical examination of the animals after challenge revealed that vaccination by both routes conferred some degree of protection, yet IV vaccination was clearly more effective in reducing the duration of virus shedding, the severity and duration of clinical disease. Taken together, these results demonstrate that SV265gE- is sufficiently attenuated upon IV vaccination in a low-titer dosis, is not reactivated after corticosteroid treatment and lastly, and more importantly, confers local protection upon challenge with a high titer of a virulent heterologous BoHV-1 isolate. Thus, this immunization strategy may be considered for the prevention of BoHV-1-associated genital disease in the field. / O presente estudo relata a avaliação da atenuação e proteção conferida pela vacinação intravaginal de bezerras com uma cepa recombinante do herpesvírus bovino tipo 1 (BoHV-1) defectiva na glicoproteína E (SV265gE-). Um grupo de seis bezerras foi vacinado com a cepa SV265gE- na submucosa da vulva (grupo IV; 106,9TCID50); quatro bezerras foram vacinadas pela via intramuscular (grupo IM; 107,6TCID50) e quatro bezerras permaneceram como controles não-vacinadas. As bezerras vacinadas pela via IV apresentaram edema e hiperemia leve e transitório na vulva; excretaram pequenas quantidades de vírus nas secreções, mas não transmitiram o vírus a uma bezerra sentinela mantida em contato. A tentativa de reativar o vírus vacinal em duas bezerras vacinadas IV pela administração intravenosa de dexametasona (0.5 mg.kg-1) no dia 65 pós-vacinação (pv) não resultou em excreção viral, recrudescência clínica ou soroconversão. No dia 65 pv, as bezerras vacinadas e as controles foram desafiadas pela inoculação genital da cepa SV-56/90 do BoHV-1.2 (107,4TCID50/animal). Após o desafio, o vírus foi detectado nas secreções das bezerras controles por 8 a 9 dias ( 8,2 dias); nas bezerras do grupo IM por 5 a 8 dias ( 6,2) e nas do grupo IV por 5 a 6 dias ( 5,2 dias). As bezerras controle desenvolveram vulvovaginite moderada (2/4) ou severa (2/4) com duração média de 11,2 dias (9 14). A enfermidade caracterizou-se por edema vulvar, congestão vulvovestibular, formação de pequenas vesículas, pústulas, que progrediram até coalescerem, erosões, placas fibrinonecróticas recobertas com exsudato fibrinopurulento. As bezerras do grupo IM desenvolveram vulvovaginite leve (1/3) ou moderada (3/4), com duração média de 11,5 dias (10 13 dias); enquanto as bezerras do grupo IV desenvolveram sinais genitais leves e transitórios (3/4) ou moderados (1/4), com duração média de 5,5 dias (4 8). A avaliação clínica pós-desafio demonstrou que a vacinação pelas duas vias (IM e IV) conferiu proteção, mas a vacinação IV foi mais efetiva na redução do tempo de excreção viral e na redução da severidade e duração dos sinais clínicos. Assim, esses resultados demonstram que a cepa SV265gE- é suficientemente atenuada para vacinação IV em dose baixa, não é reativada pela administração de dexametasona e, principalmente, confere proteção adequada frente a desafio genital com uma dose alta de uma cepa heteróloga altamente virulenta. Portanto, essa estratégia de imunização pode ser considerada para a prevenção das perdas reprodutivas causadas pela infecção genital pelo BoHV-1.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This study aimed at reproducting and characterizing the virological and clinico-pathological aspects of acute and latent genital infection by bovine herpesvirus type 1.2 (BoHV-1.2) in heifers. Four heifers were inoculated intravaginally with a Brazilian BoHV-1.2 isolate (SV-56/90) recovered from an outbreak of balanoposthitis. Virus inoculation (108.1TCID50/animal) resulted in efficient virus replication in the genital mucosa and the development of moderate to severe vulvovaginitis. The inoculated heifers shed virus in genital secretions in titers up to 107.3TCID50/mL until day 10 post infection (pi). Hyperemia, edema of vulvar and vestibular mucosa, vesicles, pustules and scabs were observed between days 1 and 10 pi. The vesicles and pustules increased in size and eventually coalesced and became covered with a mucopurulent and fibrinous exsudate. These signs increased in severity up to days 5 8 pi and progressively subsided thereafter. Dexamethasone administration at day 55 pi resulted in virus shedding in vaginal secretions of all heifers for up to 10 days. Virus reactivation was accompanied by genital signs resembling those observed during acute infection, yet less severe. Examination of the lumbar sacral ganglia and lymph nodes by PCR at day 36 postreactivation revealed the presence of latent viral DNA in the pudendal (4/4), genito-femoral, sciatic and rectal caudal (3/4) and obturator nerve ganglia (1/4); and in the sacral (3/4), deep inguinal, pre femoral, supramammary (2/4) and medial iliac lymph nodes (1/4). These results demonstrate that BoHV-1.2 DNA persists in sacral ganglia and regional lymph nodes during latency, and help in understanding the pathogenesis of acute and latent genital infection by BoHV-1.2. / O presente estudo teve como objetivos reproduzir e caracterizar os aspectos virológicos e clinico-patológicos da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 (BoHV-1.2) em bezerras. Quatro bezerras foram inoculadas no trato genital com um isolado brasileiro de BoHV-1.2 (SV-56/90) oriundo de um surto de balanopostite. A inoculação do vírus (108,1TCID50/animal) resultou em replicação viral eficiente na mucosa genital e no desenvolvimento de vulvovaginite moderada a severa. As bezerras inoculadas excretaram vírus nas secreções genitais em títulos de até 107,3TCID50/mL por até 10 dias pós-inoculação (pi). Hiperemia, edema das mucosas vulvar e vestibular, vesículas, pústulas e crostas foram observados entre os dias 1° e 10° dia pi. As vesículas e pústulas aumentaram de diâmetro, eventualmente coalesceram e tornaram-se recobertas com um exsudato mucopurulento e fibrinoso. Os sinais aumentaram em severidade do 5° ao 8° dia pi e regrediram a partir de então. Administração de dexametasona dia 55 pi resultou em excreção viral nas secreções vaginais por até 10 dias. A reativação viral foi acompanhada de sinais genitais semelhantes aos observados durante a infecção aguda, porém com menor severidade. A análise dos gânglios lombo-sacral e linfonodos regionais por PCR no dia 36 pós-reativação demonstrou a presença de DNA viral latente nos gânglios pudendo (4/4), genito-femural, ciático, retalcaudal (3/4) e obturador (1/4); e nos linfonodos sacral (3/4), inguinal profundo, pré-femural, supramamário (2/4) e ilíaco medial (1/4). Esses resultados demonstram que o DNA do BoHV-1.2 persiste nos gânglios sacrais e em linfonodos regionais durante a latência, e contribuem para o conhecimento da patogenia da infecção genital aguda e latente pelo BoHV-1.2.

Made available in DSpace on 2014-12-17T14:10:28Z (GMT). No. of bitstreams: 1 ErikaGL_DISSERT.pdf: 2372644 bytes, checksum: 71509c6c298fb6e35890d8d13548037c (MD5) Previous issue date: 2012-12-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The genital HPV infection is very common between men and women worldwide, affecting particularly young women, constituting a serious public health problem in less developed regions, favored by the poor living conditions of population. The cytology and colposcopy have notorious importance in the diagnosis of precursor lesions of cervical cancer and therefore its prevention. However, even with such diagnostic tools, the number of women who develop cervical cancer is still high. This study aims to assess the prevalence of genital tract infection by HPV in pregnant and nonpregnant women, evaluating the profile of the immune response presented by the women of these two groups in order to establish correlations among profile of immune response, presence of virus and occurrence of lesions of the uterine cervix. We analyzed specimens obtained from the cervix of 221 patients, 91 pregnant and 130 non-pregnant, aged 14-72 years. The women were subjected to colposcopic and cytologic evaluation detect possible changes in the cervix and then samples were collected in order to perform HPV detection by PCR and real-time PCR for detection of mRNA of pro-inflammatory and anti-inflammatory cytokines. In the present study, the overall prevalence of HPV genital infection was 28.1%; of which 31.9% were pregnant patients and 25.4% in non-pregnant women. Young women under 30 years and those with low educational level education showed a higher risk of HPV infection. Colposcopy showed better correlation with detection of HPV DNA by PCR, when compared to cytology. Generally, HPV infected patients, pregnant or not, exhibited reduced mRNA expression of both pro-inflammatory (IFN-&#947;, TNF-&#945;) and anti-inflammatory (IL -10) cytokines, when compared to patients not infected by HPV. Nonpregnant patients infected presented increase mRNA expression of IL-17 in patients without injury, whereas those with lesion showed higher mRNA expression of TGF-&#946;. Pregnant women without injury infected exhibited increased mRNA expression of TGF-&#946;. There was no difference in HPV prevalence between pregnant and nonpregnant women. There was a reduction of pro-inflammatory cytokines, except IL-17, in all women infected by HPV. Moreover, we observed an increase of TGF-&#946; in HPV-infected women who are pregnant or not. The results suggest that, in women in this study, HPV infection promoted changes in the profile of cytokines necessary for activation of effective immune response, possibly favoring viral persistence / A infec??o genital pelo v?rus do papiloma humano (HPV) ? muito frequente entre homens e mulheres em todo o mundo, afetando em especial mulheres jovens, constituindo-se em grave problema de sa?de p?blica nas regi?es menos desenvolvidas, sendo favorecida pelas condi??es prec?rias de vida da popula??o. A citologia onc?tica e a colposcopia t?m not?ria import?ncia no diagn?stico das les?es precursoras do c?ncer do colo uterino e, portanto, na sua preven??o. No entanto, mesmo com a disponibilidade dessas ferramentas diagn?sticas o n?mero de mulheres que desenvolvem c?ncer do colo do ?tero ainda ? elevado. Esse estudo tem por objetivo avaliar a preval?ncia de infec??o do trato genital por HPV, em mulheres gr?vidas e n?o gr?vidas, avaliando o perfil da resposta imune apresentado pelas mulheres desses dois grupos, visando estabelecer correla??es entre o perfil de resposta imune, a presen?a do v?rus e ocorr?ncia de les?es da c?rvice uterina. Foram inclu?das neste estudo 221 pacientes, sendo 91 gr?vidas e 130 n?o gr?vidas, com idade variando de 14 a 72 anos. As mulheres foram submetidas a uma avalia??o colpocitol?gica para a detec??o de poss?veis altera??es na c?rvice uterina e em seguida coletados esp?cimes para an?lise por meio da rea??o em cadeia da polimerase (PCR) convencional para detec??o do HPV e PCR em tempo real para detec??o de RNA mensageiro (RNA-m) de citocinas pr?-inflamat?rias e anti-inflamat?rias. A preval?ncia global da infec??o genital pelo HPV, encontrada neste estudo, foi de 28,1%, sendo 31,9% em pacientes gr?vidas e 25,4% nas n?o gr?vidas. As mulheres jovens com at? 30 anos e aquelas com baixa escolaridade apresentaram maior risco de ter infec??o pelo HPV. A colposcopia apresentou melhor correla??o com a detec??o do DNA do HPV por PCR, quando comparada ? citologia. De um modo geral, as pacientes infectadas pelo HPV, gr?vidas ou n?o, apresentaram uma redu??o da express?o de RNA-m tanto para as citocinas pr?-inflamat?rias (IFN-&#947;, TNF-&#945;), quanto para a citocina anti-inflamat?ria (IL-10) em rela??o ?s pacientes n?o infectadas com HPV. Nas pacientes n?o gr?vidas e infectadas, a express?o do RNA-m para IL-17 mostrou-se aumentada nas pacientes sem les?es, enquanto que, aquelas com les?o apresentaram maior express?o do RNA-m para TGF-&#946;. As mulheres gr?vidas e infectadas pelo HPV e sem les?o, apresentaram aumento na express?o do RNA-m para TGF-&#946;. N?o houve diferen?a de preval?ncia do HPV entre mulheres gr?vidas e n?o gr?vidas. Observou-se uma redu??o da produ??o de citocinas pr?-inflamat?rias, exceto IL-17, em todas as mulheres infectadas pelo HPV, e um aumento de TGF-&#946;, nas mulheres infectadas pelo HPV gr?vidas ou n?o. Os resultados sugerem que, nas mulheres deste estudo, a infec??o pelo HPV promoveu uma altera??o no perfil de citocinas necess?rias para a ativa??o de uma resposta imune efetiva, possivelmente favorecendo a persist?ncia viral

The present study was to diagnose and classify the Human Papilloma Virus (HPV) in pregnant and non-pregnant adolescents, as study the risk factors related to the infection, identify the multiples oncogenics types presents in the study people and associate the differents types of HPV with cytology and colposcopy findings. Methodologic design: the type of study was prospective, descriptive and transversal coorte. Was study 111 sexually active adolescents, between 10 and 19 years old, patients of the Gynecology Service of the Professor Alberto Antunes University Hospital of the Federal University of Alagoas (HUPAA-UFAL). Was utilized the polymerase chain reaction / restriction fragment length polymorphism - sequencing (PCR/RFLPs) to identification of the virus. Results: the viral percentual in the inferior genital tract was 27% of the cases. The molecular genotyping revealed high risk viral genetic material - HPV 16, 33, 51, 58, 66, 1S39, CP8304 and LVX100, in 28,5% of the cases, low oncogenic risk - HPV 6, 11, 53, 61 e CP141, in 40% and, the other 31,4%, considered the indeterminate type, include high and low risk virus. This more incident type is found isolated or associated to other viral types and appears in 40% of the positive cases. The viral infection rate among the pregnant adolescents was 11,7%. The HPV genital infection was associated with a past of sexually transmitted diseases and the consumption of alcohol by the adolescent. The frequency of adolescents infected by HPV, associated to low grade intraepithelial lesion, was 5,0%. No associations were observed between the specific types of HPV and the cytology and colposcopy findings among the studied adolescents. / O objetivo do presente estudo foi para diagnosticar e classificar o papilomavírus humano (HPV) em adolescentes grávidas e não grávidas, bem como estudar os fatores de risco para a infecção, identificar os diversos tipos oncogênicos presentes na população estudada e associar os diferentes tipos de HPV com achados da citologia e colposcopia. Desenho metodológico: o tipo de estudo foi prospectivo, descritivo e de coorte transversal. Foram estudadas 111 adolescentes sexualmente ativas, entre 10 e 19 anos, atendidas no Serviço de Ginecologia do Hospital Universitário Prof. Alberto Antunes da Universidade Federal de Alagoas (HUPAA-UFAL). Utilizou-se a reação de polimerase em cadeia/polimorfismo de comprimento de fragmentos de restrição - sequenciamento (PCR/RFLPs), para a identificação do vírus. Resultados: o percentual do vírus no trato genital inferior foi de 27%. A genotipagem molecular revelou material genético viral de alto risco - HPV 16, 33, 51, 58, 66, 1S39, CP8304 e LVX100, em 28.5% dos casos, enquanto que os de baixo risco oncogênico - HPV 6b, 11, 53, 61 e CP141, em 40%. Os demais 31,4%, foram do tipo considerado indeterminado, que incluem vírus de alto e baixo risco. Esse tipo mais incidente encontra-se isolado ou associado a outros tipos virais e aparece em 40% do total dos casos positivos. A taxa de infecção viral entre as gestantes foi de 11,7%. A infecção genital pelo HPV teve associação com o passado de doenças sexualmente transmissíveis e com o consumo de álcool pela adolescente. A freqüência de adolescentes infectadas pelo HPV, associada à lesão intra-epitelial de baixo grau foi de 5,0%. Não foram observadas associações tipo-específicas do HPV com os achados da citologia e da colposcopia entre as adolescentes estudadas.