Submitted by Rosangela Silva (rosangela.silva3@unioeste.br) on 2017-08-11T17:24:39Z No. of bitstreams: 2 Taina de Souza 2017.pdf: 1552829 bytes, checksum: 891fdf36e760cfb11f85562d5918a74e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-08-11T17:24:39Z (GMT). No. of bitstreams: 2 Taina de Souza 2017.pdf: 1552829 bytes, checksum: 891fdf36e760cfb11f85562d5918a74e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-02-16 / (Poaceae of the campestre formations from Guartelá State Park, Paraná, Brazil). Considered one of the greatest families among the angiosperms and with great ecological amplitude, Poaceae species can be found both in forest as well as in campestre formations. The goal of the present study was to perform the first floristic survey of Poaceae in one of the last remnants native vegetation of campestre formations in Paraná, the Guartelá State Park (GSP), besides elaborate dichotomous keys in order to identify the genera and species. The survey was carried out based on field collecting and database search. The dichotomous keys were elaborated using qualitative and quantitative characters of the genera and species. In the GSP, the family is represented by 54 species belonging to 29 genera, Paspalum L. (8 spp.), Andropogon L. (5 spp.) and Eragrostis Wolf (4 spp.) being the ones that presented the greater number of species. The number of species cited previously to the GSP increased in 29 names and from the total (54) five are considered exotic, five endemics to Brazil and two are categorized as least concern to conservation, occurring both in dry and wet areas, highlighting the importance of the GSP microenvironments conservation. Information regarding the distribution, habitat and species conservation are presented. Keywords: Campos Gerais, floristic, grasses, grassland vegetation, Poales. / (Sinopse de Poaceae das formações campestres do Parque Estadual do Guartelá, Paraná, Brasil). Considerada uma das maiores famílias entre as Angiospermas e com grande amplitude ecológica, Poaceae tem suas espécies encontradas tanto em formações florestais como em campestres. O presente estudo teve como objetivo realizar o primeiro levantamento florístico de Poaceae em uma das últimas vegetações nativas remanescentes de formações campestres no Paraná, o Parque Estadual do Guartelá (PEG), além de elaborar chaves dicotômicas para identificação dos gêneros e espécies. O levantamento foi realizado com base em coletas a campo e busca em banco de dados. As chaves dicotômicas foram elaboradas utilizando caracteres qualitativos e quantitativos dos gêneros e espécies. No PEG, a família esta representada por 54 espécies pertencentes a 29 gêneros, sendo Paspalum L. (8 spp.), Andropogon L. (5 spp.) e Eragrostis Wolf (4 spp.) os que apresentaram maior número de espécies. Ampliou-se o número de espécies citadas anteriormente para o PEG em 29 nomes e do total (54) cinco são consideradas exóticas, cinco endêmicas para o Brasil e duas estão categorizadas como pouco preocupantes para conservação, tendo ocorrência tanto em áreas drenadas quanto úmidas, destacando a importância da conservação dos microambientes do PEG. São apresentadas informações acerca da distribuição, hábitat e conservação das espécies.

Resumo: O presente trabalho teve como objetivo analisar a viabilidade de uso do anidrido naftálico (NA) no tratamento de sementes de espécies gramíneas, assim como no tratamento de toletes de Saccharum spp, contra os efeitos causados por herbicidas inibidores da síntese de carotenóides. O trabalho foi constituído de quatro etapas, sendo as etapas 1 e 3 conduzidas a campo e em casa de vegetação no NUPAM/FCA/UNESP, Botucatu-SP, e as etapas 2 e 4, em estufa plástica e a campo na ESAPP e Empresa Guacho S/A, Paraguaçu Paulista-SP. Nas etapas 1, 2 e 3, de forma geral, foi estudado para as espécies Sorghum bicolor; Penisetum americanum, Panicum maximum, Brachiaria brizantha e Zea mays o efeito da combinação ou não do tratamento de sementes com NA submetidas ao uso dos herbicidas isoxaflutole (IFT) e clomazone, utilizando-se diferentes dosagens, tipos de solos e formas de aplicação. Na etapa 4 foi estudada a combinação de NA no tratamento de toletes da variedade SP 813250 (testemunhas sem NA; aplicação por imersão em solução de 3,0 g L-1 por duas horas; pulverização de 25 g ha-1 de NA sobre os colmos) e herbicida (testemunha; 112 g ha-1 de isoxaflutole; 1440 g ha-1 de metribuzin), e mais um tratamento adicional, onde os toletes foram imersos apenas em água sem adição de NA. O uso de NA (0,50% de p/p) no tratamento de sementes reduziu a intoxicação do híbrido Colorado 32 contra a ação do isoxaflutole, em pré-emergência da cultura, assim como favoreceu o aumento da produtividade dos híbridos Cargill 435 e Colorado 32, na ausência ou presença do efeito herbicida. O tratamento de sementes com NA (0,50% de p/p) protegeu a espécie S. bicolor BR 304 contra os sintomas visuais de intoxicação do isoxaflutole (112 g ha-1) e clomazone (1000 g ha-1)... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The present work had as objective to analyze the viability of naphthalic anhydride (NA) use in grass crops, through seed treatment, as well as in stalks treatment of Saccharum spp, against the effects caused by carotenoid synthesis inhibitor herbicides. The work was constituted by four step, being the 1st and 3rd ones carried out in field conditions and glasshouse at the NUPAM/FCA/UNESP, Botucatu - São Paulo State; 2nd and 4th step were conducted in plastic greenhouse and field at the ESAPP and Guacho S/A, Paraguaçu Paulista - São Paulo State. In a general way, 1st, 2nd and 3rd step was studied Sorghum bicolor; Penisetum americanum, Panicum maximum; Brachiaria brizantha and Zea mays L. species in the combination with the effect or not of seed treatment with NA submitted to the isoxaflutole and clomazone, being used different doses, kinds of soils and application forms. In 4th step, treatments included the combination of three conditions of NA stalk treatment, SP 813250 variety (check without NA; application by immersion in solution of 3,0 g L-1 of NA for two hours; spraying of 25 g ha-1 of NA over the stalks) and three herbicide conditions (check without herbicide; 112 g ha-1 at isoxaflutole; 1440 g ha-1 at metribuzin), and an additional treatment, where the stalks were just put inside water without NA. Seed treatment with NA (0,50% of w/w) reduced Colorado 32 hybrid intoxication by isoxaflutole action in preemergence of the crop, as well as favored the increase of Cargill 435 and Colorado 32 hybrids yield, in the absence or presence of herbicide effect. Seed treatment with NA (0,50% of p/p) protected S. bicolor BR 307 against the visual isoxaflutole (112 g ha-1) and clomazone (1000 g ha-1... (Complete abstract, click electronic address below) / Orientador: Edivaldo Domingues Velini / Coorientador: Sílvio José Bicudo / Banca: Dagoberto Martins / Banca: Julio Cesar Durigan / Banca: Rubem S. de Oliveira Junior / Banca: Reginaldo Teodoro de Souza / Doutor

O rápido declínio da qualidade nutricional de P. maximum com o avanço da maturidade é um dos maiores limitantes do desempenho animal em pastagens. Devido ao modo de reprodução apomítica da espécie, a engenharia genética surge como importante ferramenta para o desenvolvimento de novas cultivares. Sendo assim, a identificação de genes responsáveis pela queda da digestibilidade da fibra é um passo importante para a geração de cultivares transgênicas. Objetivou-se neste estudo determinar a produtividade, composição bromatológica, digestibilidade in vitro das frações folha e colmo de 11 genótipos de P. maximum colhidos em três idades de corte: 30, 60 e 90 dias de crescimento, bem como quantificar a expressão de seis enzimas da via de lignificação da parede celular. Objetivou-se ainda a classificação dos genótipos em grupos de acordo com suas características produtivas e de qualidade nutricional. Utilizou-se delineamento de blocos ao acaso, em esquema de parcela subdividida, com três repetições, no qual a parcela experimental foi composta de seis linhas com 4 m de comprimento, espaçadas de 0,5 m, sendo a data de corte a parcela e os genótipos as sub-parcelas. A produção de MS diferiu entre os genótipos somente com 90 dias de crescimento. Houve ainda variação entre os genótipos quando à porcentagem de folhas, colmos e material morto. Os genótipos não diferiram quanto à composição química e digestibilidade da fração folha, porém apresentaram grande variação quanto à composição química e digestibilidade da fração colmo. A fração colmo apresentou maiores valores de FDN, FDA e lignina, e menores valores de PB do que a fração folha. Entretanto, a fração colmo apresentou maior digestibilidade da MS com 60 dias de crescimento e maior digestibilidade da FDN com 30 e 60 dias de crescimento. O agrupamento detectou 4 grupos de genótipos de acordo com produtividade e DIVFDN do colmo. Os acessos PM39 e PM47 se destacaram por boa produtividade, elevada digestibilidade da FDN do colmo, enquanto as cultivares Milênio e Mombaça apresentaram alta produção de massa, porém baixa digestibilidade da fibra do colmo. A cultivar Massai e os acessos PM39 e PM47 foram selecionados como genótipos com boa manutenção da digestibilidade da fibra (LENTA), enquanto os genótipos Tanzânia, Milênio e Mombaça foram selecionados como genótipos com rápida queda da digestibilidade da fibra do colmo (RÁPIDA) para quantificação da expressão gênica. Foi realizada uma quantificação da expressão relativa de seis genes de interesse (CCR, COMT, C4H, 4CL, CAD e PAL) e um gene controle (GAPDH) nas três idade de corte. Houve interação tratamento idade para os genes C4H e COMT, com aumento da expressão dos genes de 30 para 60 dias de crescimento somente no grupo de RÁPIDA queda da digestibilidade. No grupo com LENTA queda da digestibilidade não houve alteração da expressão com 60 dias. O gene PAL mostrou comportamento semelhante, porém a interação tratamento idade não foi significativa. Estas características posicionam estes três genes como possíveis moduladores da digestibilidade do colmo de P. maximum. Conclui-se que a maturidade tem maior efeito sobre a qualidade nutricional da fração colmo do que sobre a fração folha e que há grande variação entre os genótipos em relação ao efeito da maturidade sobre a digestibilidade da fração colmo, indicando a possibilidade de seleção de cultivares com maior digestibilidade de colmos, e a dificuldade de se selecionar genótipos com maior digestibilidade de folhas. Identificou-se os genes C4H, COMT e PAL como possíveis candidatos à engenharia genética para produção de cultivares transgênicas. / The rapid decline of nutritional quality of P. maximum with advanced maturity is an important limiting factor for animal performance in pastures. Because of the apomitic reproduction of the species, genetic engineering arises as an important tool for development of new cultivars. Therefore, the identification of genes responsible for the decline in fiber digestibility is an important step towards generating transgenic cultivars. The objective of this study was to determine the yield, chemical composition, in vitro digestibility of leaf and stem fractions of 11 P. maximum genotypes harvested at three stages: 30, 60 and 90 days of regrowth, as well as to quantify the expression of six enzymes from the cell-wall lignification pathway. Another objective was to classify the genotypes in groups according to their productive and nutritional characteristics. A randomized block design in a split plot arrangement with three replications was used, in which the experimental plot was composed of six lines with 4 m length, spaced 0.5 m, being the harvest age the plot and the genotypes the sub-plots. The DM production differed between genotypes only after 90 days of regrowth. There was variation among genotypes in the percentage of leaves, stems and dead material. The genotypes did not differ in chemical composition and digestibility of the leaf fraction, but there was great variation in chemical composition and digestibility of the stem fraction. The stem fraction had higher NDF, ADF and lignin content, and lower CP content than the leaf fraction. However, the stem fraction had higher DM digestibility with 60 days of regrowth, and higher NDF digestibility after 30 and 60 days. The clustering procedure separated the genotypes in four groups cording to DM yield, stem NDF digestibility and percentage of leaves. The accessions PM39 and PM47 stood out for their good productivity and high stem NDF digestibility, while the cultivars Milênio and Mombaça had high DM yield but lower stem NDF digestibility. For quantification of gene expression, the Massai cultivar and the accessions PM39 and PM47 were selected as genotypes with good maintenance of fiber digestibility (SLOW), while the genotypes Tanzânia, Milênio and Mombaça were selected as ones with fast decline in stem fiber digestibility (FAST). The relative expression of six genes of interest (CCR, COMT, C4H, 4CL, CAD and PAL), and one control gene (GAPDH) was determined in the three harvested ages. There was a treatment age interaction for the C4H and COMT genes, with an increase in gene expression from 30 to 60 days of regrowth only in the group with FAST decline in digestibility. In the group with SLOW decline in digestibility, there was no difference in expression from 30 to 60 days of regrowth. The gene PAL had similar profile of expression; however the treatment age interaction was not significant. These characteristics place these three genes as possible modulators of stem digestibility in P. maximum. We conclude that maturity has a great effect over the stem nutritional quality than over leaves, and there was great variation among genotypes in the effect of maturity on stem fiber digestibility, indicating the possibility to select cultivars with higher stem digestibility, and also the difficulty to select genotypes with higher leaf digestibility. We identified the genes C4H, COMT and PAL as possible candidates for genetic engineering aiming the production of transgenic cultivars.

Increased fire frequency, livestock grazing and other disturbances have caused type-conversion of native chaparral to non-native annual grasslands. Competition with non-native annual grasses hinders shrub establishment, presenting challenges in restoration. This study examined the feasibility of chaparral restoration in a Mediterranean-type climate, on a type-converted landscape in San Timoteo Canyon, Riverside County, California. The objectives of this study were to (1) compare the effectiveness of the broad-spectrum herbicide, glyphosate, to that of the grass specific herbicide, fluazifop, at eliminating non-native annual grasses; (2) compare the success of seeding or planting native seedlings as a means of restoration; (3) analyze the soil seed bank to determine if there was a relict native species seed bank on site; (4) test the field application of smoke-water as a technique to manipulate the soil’s native shrub seed-bank for restoration. A factorial design was utilized, with three replicates: four restoration treatments (no restoration, smoke-water application, seeding, and planting seedlings) across three herbicide treatments (a January glyphosate application followed by an early-March fluazifop treatment, an early-March fluazifop treatment only, and a control treatment with no herbicide). Glyphosate application followed by fluazifop application proved to be more effective at reducing non-native annual grasses and benefitted shrub establishment more effectively than the fluazifop and control treatments. The application of glyphosate in winter promoted shrub establishment and growth, and increased soil moisture, while preventing the springtime increase in grass cover that was seen in the control and fluazifop-only treatments. The four chaparral species that were planted (Adenostoma fasciculatum, Eriogonum fasciculatum, Quercus berberidifolia, Rhus ovata) did not behave similarly. No Quercus berberidifolia individuals survived. Eriogonum fasciculatum did not exhibit differences in survival in any herbicide treatments, but exhibited higher live plant canopy volume in plots that received glyphosate with fluazifop follow-up treatments. Adenostoma fasciculatum and Rhus ovata showed higher survival in plots that received glyphosate with fluazifop follow-up treatments; however, neither exhibited differences in live plant canopy volume between herbicide treatments. These results persisted until the end of the study, 14 months after planting. Seed application of seven species (Adenostoma fasciculatum, Artemisia californica, Eriogonum fasciculatum, Gutierrezia sarothrae, Quercus berberidifolia, Rhus aromatica, and Rhus ovata) was not successful at this study site. Seed bank analysis revealed that there was a high density of non-native seeds present, and that the relict native shrub seed bank was insufficient for restoration on this site. Smoke-water application at the study site proved to be ineffective at manipulating the soil seed bank, as no chaparral species emerged. Results from this study suggest that winter glyphosate application followed by a fluazifop application in a Mediterranean-type climate may benefit shrub re-establishment more than spring fluazifop-only treatments. Results indicate that winter herbicide treatment will eliminate competitive non-native annual grasses early in the growing season, resulting in increased soil moisture availability for chaparral shrubs to utilize. Winter glyphosate application, supplemented with the planting of seedlings, could be a successful technique to re-establish chaparral shrubs on type-converted slopes.

Thesis (M.Sc. (Pasture Science)) -- University of Limpopo, 2014 / Forage sorghum is widely grown in South Africa as annual summer forage to supplement pasture production for sheep, beef and dairy cattle. A number of sorghum cultivars are available commercially, and periodically some cultivars are added while others are withdrawn from the market. The potential yield figures and the nutritive value of these forage sorghum cultivars are generally not known. The management practices that improve forage sorghum production and quality include the time of planting and time of harvesting. The genetic makeup of different forage sorghum cultivars also accounted for a portion of the production and quality. A field experiment was conducted at Dewageningsdrift Experimental Farm (DWD), Moloto, Gauteng and Nooitgedacht Agricultural Development Center (NGD), Ermelo, Mpumalanga to study the influence of planting date on the growth potential of different forage sorghum cultivars. Three planting dates were used: mid-December 2006, mid-January 2007 and mid-February 2007. Thirteen different cultivars were incorporated in the trial to evaluate influence of the breeding history. The cultivars were defoliated at three different stages: cut repeatedly at six weekly intervals (Dt 1), cut repeatedly when it reached a grazing stage (± 800 mm high) (Dt 2) and once at the silage stage (soft dough) (Dt 3). At DWD the average total dry matter (TDM) productions, for the six week cutting treatment (Dt 1), were 10760 kg/ha, 5195 kg/ha and 1944 kg/ha for December, January and February planting date respectively. For the same treatment, at NGT, the average TDM productions were 6396 kg/ha and 1737 kg/ha for December and January respectively. The February planting resulted in the poor germination and seedling emergency. The seedlings did not survive due to low temperatures. The minimum of 13 ºC and 11.8 ºC as well as the maximum of 24.1 ºC and 23.0 ºC in February and March were below the required germination temperature (15 ºC). The highest producers that is available in the market were Jumbo, Sentop, Piper, Kow Kandy, and Sugargraze. Defoliated repeatedly at grazing stage (Dt 2), at DWD, resulted in average TDM productions of 8541 kg/ha, 4950 kg/ha and 2683 kg/ha for December, January and February, respectively. At NGT the average TDM productions were 7769 kg/ha and 3010 kg/ha for December and January respectively. The highest producers were Jumbo, Kow Kandy, Piper, Sentop and Sugargraze. The average TDM productions at the silage stage (Dt 3), at DWD, were 17923 kg/ha, 15015 kg/ha and 2529 kg/ha for December, January and February respectively. At NGT the average TDM production iii was 11856 kg/ha and 5350 kg/ha for December and January, The highest producers were Jumbo, Sugargraze, Kow Kandy, Sentop and Kow Kandy.December planting proved to be the best planting date for optimum DM production, compared to later plantings in January and February. Keywords Forage sorghum, cultivars, planting dates, defoliation stages, grazing stage, silage

Plant spatial patterns have been studied to gain insight into plant interactions such as competition and facilitation (positive plant interactions). The stress gradient hypothesis predicts that as environmental stress increases facilitation dominates, while competition dominates in less stressful conditions. Beneficial plants (nurses) can create favorable abiotic conditions for subanopy plants. Additionally, palatable herbaceous species growing under nurse shrub canopies benefit from physical protection. I investigated spatial associations between Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis) and three native grasses (Poa secunda, Elymus elymoides, and Pseudoroegneria spicata) across a rainfall gradient in the Great Basin, USA. I also explored the effect of grazing on grass-shrub spatial associations. I hypothesized that positive shrub-grass spatial associations would become more frequent at lower rainfall levels; I further hypothesized that 1) at intermediate levels of stress, positive grass-shrub spatial associations would dominate and 2) at extreme levels of stress, positive grass-shrub spatial associations and interactions would no longer dominate. At high moisture stress, the addition of grazing stress may limit the nurse’s ability to provide to benefits to subcanopy plants. Cover of P. secunda was greater in shrub canopy microsites than interspaces at low to moderate levels of rainfall. Cover and density of E. elymoides were greater in sagebrush canopies over most rainfall levels. Elymus elymoides and P. spicata were taller and narrower in basal width and less likely to be grazed in canopy versus interspace microsites. I next investigated the effects of grazing intensity over a rainfall gradient and found a significant interaction of rainfall and microsite on P. secunda cover. Poa secunda formed positive interactions with A. tridentata at lower rainfall levels, regardless of grazing intensity. Its cover was significantly greater in interspaces at high rainfall compared to low rainfall sites. Elymus elymoides density was greater in canopy vs. interspace microsites, regardless of rainfall level or grazing intensity. Plant spatial associations can indicate which nurse microsites are favorable to plant growth and may improve seeding or planting success during ecological restoration. My results suggest that exploiting sagebrush canopy microsites for restoration of native perennial grasses would improve plant establishment, growth or survival particularly in drier areas.

This thesis examines the hypothesis that, in southern New South Wales and northeast Victoria, Australia, palatable taxa of E. curvula offer advantages that complement those of the species that are traditionally sown in temperate pastures in a landscape context.This hypothesis was based on a review of literature showing that, prior to European settlement, the vegetation, the landscape and the climate were broadly in balance, and the wateruse pattern of the vegetation of southeastern Australia resulted in water being used more-or-less completely by the end of summer. This maximised the capacity of the soil to take up and store water during autumn and winter.Three grazing experiments and one spaced-plant species evaluation study were used to assess the role of summer-growing, C4 Eragrostis curvula in pastures in the temperate zone of southeastern Australia.Issues relating to pasture production and the productivity of wool-growing sheep were investigated. Factors affecting the sustainability of the pastures and their potential on and off site impacts were emphasised.Modelling was used to explore issues of water use, arising from the grazing experiments. It is concluded that the persistence, production, water use patterns, and the adaptability of palatable varieties of E. curvula make it a useful and complementary addition to the range of species that are currently available for use as sown pastures in southern Australia. / Doctor of Philosophy (PhD)

Bibliography: leaves 82-89. This study is aimed at providing information on control measures against Gaeumannomyces graminis var. tritici (abbreviated to Ggt) in annual pastures across southern Australia. The impact of timing of herbicide application, the impact of variation in timing of rainfall patterns, as well as herbicide application on the control of Ggt are also discussed. Additional experiments examine the ability of grass genera to host and carry over Ggt.

Le collage est largement utilisé dans l'industrie automobile comme mode d'assemblage de tôles grasses. Or, le lubrifiant initialement présent entre la tôle et l'adhésif peut dans certains cas poser problème et conduire à une déchéance de l'assemblage collé (avant ou après vieillissement). L'objectif de cette étude est de fournir des démarches et méthodes permettant d'acquérir une meilleure compréhension des phénomènes et mécanismes régissant le collage de tôles huilées. Le système étudié est semi industriel (tôle galvanisée, huile et cycle de cuisson industriels, adhésif époxydique modèle).Basée sur l'analyse de l'assemblage avant et après cuisson, une approche multitechnique a permis de montrer que le contact tôle/adhésif s'établit par diffusion du lubrifiant dans l'adhésif. Cette approche a été complétée par une étude dynamique des phénomènes. Ainsi, la formation des interphases substrat/huile/adhésif a été suivie de façon continue au cours du cycle thermique de cuisson de l'adhésif. Ceci nous a permis de mieux appréhender le comportement et devenir de l'huile au sein de l'assemblage, par exemple a- en établissant la chronologie des phénomènes de diffusion d'huile et de réticulation de l'adhésif, b- en déterminant l'influence de l'huile sur les cinétiques et mécanismes de réticulation et de formation des interphases, et c- en identifiant les paramètres clefs de la compatibilité huile/adhésif (viscosité de l'adhésif, charges, solubilité et évolution des masses moléculaires ). Enfin, des essais mécaniques (torsion et traction cisaillement, avant et après vieillissement hygrothermique) ont montré une faible influence de l'huile sur la robustesse des assemblages. Au delà de la caractérisation de notre système particulier, nos travaux ont été menés de façon à fournir et valider des démarches et appareillages utilisables pour une large gamme de systèmes modèles ou industriels.

Lepidium latifolium L. (perennial pepperweed, LEPLA) is an exotic invader throughout western North America. At Malheur National Wildlife Refuge (MNWR) in southeast Oregon, it has invaded about 10% of meadow habitats that are important for wildlife. This study's objective was to determine the most effective and least environmentally harmful treatment to control this weed and restore native vegetation using integrated pest management techniques. During summer 1995, nine 0.24-ha plots in three meadows infested with L. latifolium at MNWR were randomly assigned to a treatment with metsulfuron methyl herbicide, chlorsulfuron herbicide, disking, burning, herbicide (metsulfuron methyl or chlorsulfuron) then disking, herbicide (metsulfuron methyl or chlorsulfuron) then burning, or untreated. Changes in L. latifolium ramet densities and basal cover of vegetation, litter, and bare soil were evaluated in 1996 and 1997. Sheep grazing was evaluated as a treatment for reduction in flower production along roadsides and levees during summer 1997. Revegetation treatments of seeding, transplanting or natural (untreated) revegetation were attempted at plots treated with chlorsulfuron, disking, chlorsulfuron then disking, and at untreated plots from October 1996 through September 1997. Chlorsulfuron was the most effective control treatment with greater than 97% reduction in L. latifolium ramet densities two years after treatment. Metsulfuron methyl was an effective control (greater than 93% reduction) for one year. Disking was ineffective. Burning was ineffective at the one site where sufficient fine fuels existed to carry fire. Herbicide treatments were associated with increased grass and reduced forb cover. Disking was associated with reduced grass and litter cover. Disking combined with either herbicide treatment was associated with reductions in all plant cover (49 to 100%), increased bare ground, and invasion by other weedy species such as Cirsium arvense (L.) Scop. (Canada thistle, CIRAR) and Bromus tectorum L. (cheatgrass, BROTE). Ungrazed L. latifolium averaged 4513 flowers per ramet. Sheep grazing reduced L. latifolium flower production by at least 98%. Revegetation treatments were unnecessary in sites treated with chlorsulfuron and were ineffective at all treatment sites. / Graduation date: 2000