Background: The blue mussel, Mytilus edulis L., is an important contributor to the shellfish sector of Scottish aquaculture, with 7,270 tonnes worth £8.8 million being produced for the year 2015. Since 2010, production values have fluctuated as a result of inconsistent spat settlement, several business closures, and heightened levels of marine toxins in some areas. On Scotland’s west coast, some farms (most notably Loch Etive) have suffered production losses from the appearance of non-marketable mussels with particularly fragile shells and poor quality meat. Recent research has demonstrated that these undesirable traits have a genetic factor, linked to the presence of a non-native but related species Mytilus trossulus (Gould, 1850) and often its hybrids with the native M. edulis. M. trossulus has been classed as a commercially damaging species under Scottish law, but there is insufficient data on hybridisation and introgression patterns in Scottish mussel populations to evaluate any possible impacts this could have on production. Existing research has focused on single locus genotyping to identify Mytilus spp. and their hybrids in Scotland. By instead utilising multilocus genotyping, introgression could be identified and a better understanding of population structure could be gained, with implications for management to maintain productivity and profitability. The aim of the research presented here was to develop and validate a suite of new species diagnostic markers for multilocus genotyping of field populations of Scottish mussels, thereby establishing a more complete picture of the taxonomic relationships between species than previous studies have permitted. Results: Analysis of SNPs identified with RADseq confirmed the presence of three genetically distinct Mytilus species in Scotland: M. edulis, M. galloprovincialis and M. trossulus. RADseq and KASP genotyping technology successfully identified and validated a suite of 12 highly robust diagnostic SNP markers for multilocus genotyping of Mytilus mussel populations. These markers permitted more comprehensive genotyping than previous studies had, allowing presumed pure species individuals to be distinguished from first generation (F1) hybrids and introgressed (FX) genotypes in reference populations, and subsequently presented the possibility of exploring introgression in a wider scale study. Multilocus genotyping of mussel populations from around Scotland revealed widespread introgression of M. edulis with both M. galloprovincialis and M. trossulus. No pure M. galloprovincialis was identified and pure M. trossulus was restricted to a single site in Loch Etive, possibly part of a relict population. F1 hybrids between M. edulis and M. trossulus were identified in Loch Etive and in Loch Fyne on the west coast. This was evidence of ongoing hybridisation and suggested an active hybrid zone existed in Scotland, something that previous single locus genotyping studies had not acknowledged. A link between shell fragility and M. trossulus introgression was recognised at a single site outside of Loch Etive, but this was not apparent anywhere else and the actual causes of shell fragility remain unevaluated. There was a clear difference between the genetics of most farmed stock and wild populations, which indicated an anthropogenic effect on introgression and subsequent species composition, and had implications for future farm site selection and broodstock sourcing. Temporal species composition in Loch Etive differed over a short time period, but high proportions of M. trossulus alleles were observable some 25 months after a major fallowing event had taken place. Pure M. trossulus was also identifiable, which was consistent with the presence of an established population of M. trossulus existing in this area. Conclusion: Multilocus genotyping has produced a more in depth picture of species diversity in Scottish mussel populations. SNP assays revealed widespread introgression between three genetically distinct species – M. edulis, M. galloprovincialis and M. trossulus – and furthermore recognised that, to date, single locus genotyping has overestimated the abundance of pure Mytilus mussels in Scottish waters. However, this hitherto unidentified genetic complexity does not appear disadvantageous to mussel production, despite the prevalence of M. trossulus introgression among farmed populations, and it is somewhat unlikely that genetics are the sole cause of undesirable shell characteristics among Mytilus spp. mussels.

In the nature, crayfish are represented like predators or a part of food base of some fish. Also in free waters they acts as detrivors and are an important element in the nutrient and energy circle in aquatic ecosystem. The crayfish weren't able to avoid negative impacts of anthropogenic activities such as water pollution and devastation of habitats. For these reasons reproduction of the crayfish is necessary in hatcheries and farms. The main aim of my work was to study the reproductive biology of the crayfish focused on the process of mating, the interval between mating and laying eggs, fertilization and morphological structure of the male and female gametes. Among the secondary objectives there was included using of repeated electrostimulation of the crayfish and the possibility of hybrids of the noble crayfish (Astacus astacus) and the narrow-clawed crayfish (Astacus leptodactylus). The observation of the period of reproduction was realized on representativies of the noble crayfish and the narrow-clawed crayfish throughout natural mating in the tanks. The structure of gametes was observed on the samples taken from thenoble crayfish (Astacus astacus), the narrow-clawed crayfish (Astacus leptodactylus), the signal crayfish (Pacifastacus leniusculus), the red swamp crayfish (Procambarus clarkii) and spinycheek crayfish (Orconectes limosus). The experiments were carried out during the autumn season in 2012 and 2013. The results of observations of the reproduction of crayfish made us clear as for the period and intervals for all phases of the process of mating crayfish. For females the noble crayfish and the narrow-clawed crayfish was found oviposition without the presence of males. Their individual components have been described by using ultrastructure of males and female gametes. Repeated electrostimulation showed at the family Astacidae as a suitable tool for collecting large mass of sperm material. Hybridization experiment proved that it can occur to mate the two species of crayfish in areas where they occur simultaneously. These experiments helped to further knowledge of reproductive biology of crayfish.

This thesis is focused on the dispersal, distribution, and genetic diversity of the taxonomically highly diverse Melampyrum subalpinum group. A complete revision of the localities of M. subalpinum in the Czech Republic and Slovakia is presented. The genetic variation and population structure of the M. subalpinum group across its distribution range is described based on allozymes, nuclear and chloroplast gene sequencing, and genome size. Signs of historical hybridization with M. nemorosum were found in some populations. A comparison of seed dispersal by ants between the co-occurring M. subalpinum and M. pratense and the influence of differences in this process are presented. A new mean of seed dispersal (endozoochory) is introduced for Melampyrum. Myrmecochorous dispersal distances are tested and Holocene migration possibilities are discussed taking into account endozoochory.

p. 1-108 / Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-03-14T18:43:20Z No. of bitstreams: 1 5555.pdf: 860675 bytes, checksum: 6e4c1dc98d53482ac492c3b6c35778ef (MD5) / Approved for entry into archive by Tatiana Lima(tatianasl@ufba.br) on 2013-04-12T18:46:21Z (GMT) No. of bitstreams: 1 5555.pdf: 860675 bytes, checksum: 6e4c1dc98d53482ac492c3b6c35778ef (MD5) / Made available in DSpace on 2013-04-12T18:46:21Z (GMT). No. of bitstreams: 1 5555.pdf: 860675 bytes, checksum: 6e4c1dc98d53482ac492c3b6c35778ef (MD5) Previous issue date: 2005 / A economia solidária, na sua multiplicidade, tem sido apontada por muitos pesquisadores como um campo rico em evidências empíricas de formas de gestão mais humanizadoras e voltadas para a emancipação humana. Este trabalho voltou o olhar para um sujeito, Luciene, que num dado momento de sua trajetória de vida, começou a se inserir em organizações desse campo. O seu objetivo foi identificar as influências dessa inserção nas transformações percebidas em sua identidade. Considerou a identidade não como locus de uma substância imutável, mas como movimento, memória e desejo, ser e vir-a-ser. Para acessar esse objeto de pesquisa, utilizou como recurso metodológico a história de vida, construída a partir do relato de Luciene sobre sua trajetória. O resultado desse relato, de acordo com a citada metodologia não foi a experiência vivida em estado puro, mas a realidade cognoscível à Luciene, a partir do(s) sentido(s) atribuído(s) por ela à(s) sua(s) experiência(s). Propôs a análise da narrativa, a partir de quatro personagens que vão se expressando ao longo da vida de Luciene. Recorreu, então, à noção de dialética para compreender a forma como as transformações ocorreram em sua identidade, permitindo a expressão “das quatro Lucienes”. Em seguida, partiu para a delimitação do campo da economia solidária, enfatizando a hibridação de economias e as possibilidades de atividades humanas que decorrem dessa hibridação. No plano analítico, a manifestação destas atividades no relato de Luciene foi investigada com o objetivo de entender se, de alguma forma, elas favoreceram a sua transformação identitária. Posteriormente, numa tentativa de apontar o sentido orientador das transformações percebidas, utilizou um modelo analítico que permitiu a caracterização das atividades de cada personagem vivida por Luciene. Por último, analisou, à luz das categorias auto-realização e entendimento, a expressão em cada personagem desses fatores emancipatórios. Concluiu que a inserção de Luciene em organizações solidárias, se não determinou as transformações percebidas em sua trajetória, ao menos as favoreceu, produzindo condições objetivas e subjetivas que direcionam estas mudanças no sentido da sua humanização e emancipação. / Salvador

Les changements globaux ont en partie pour effet de modifier les aires de répartition géographique des espèces. Les interactions nouvelles entre espèces n’ayant jamais été en contact peuvent potentiellement mener à des cas atypiques de reproduction, notamment l’hybridation. Ce phénomène peut avoir des implications épidémiologiques fortes car il peut conduire à la genèse de pathogènes hybrides. La combinaison du matériel génétique d’espèces distinctes peut conférer de meilleures capacités à la progéniture (vigueur hybride ou hétérosis), pouvant à terme potentiellement mener à des changements adaptatifs et à l'émergence de pathogènes dans des zones non endémiques, ce qui en fait une menace émergente à l’échelle mondiale. Ce travail de thèse se focalise sur la schistosomiase, seconde maladie parasitaire humaine et sa récente émergence en Europe (Corse, France). Après l’identification et la caractérisation génomique d’un parasite hybride entre deux agents distincts de la maladie, S. haematobium chez l’homme et S. bovis chez les bovins, nous avons mené une approche intégrative afin de caractériser à plusieurs échelles les capacités invasives et la virulence de tels parasites. A partir de souches du terrain, nous avons mis en place un protocole d’évolution expérimentale visant à générer des hybrides de première et deuxième générations au laboratoire. Nous avons analysé les modifications de traits d’histoire de vie de ces parasites ainsi que les conséquences moléculaires (génomique et transcriptomique) de ce « clash génomique » et nous montrons que l’hybridation peut être une force évolutive majeure pour les parasites. / Global changes contribute in modifying species geographical distribution. New interactions between species that have never been in contact before can potentially lead to atypical cases of reproduction, including hybridization. This phenomenon can have strong epidemiological consequences as it can potentially lead to the genesis of hybrid pathogens. The combination of genetic material of distinct species can confer increased capacities to the offspring (hybrid vigor or heterosis), eventually leading to adaptive changes and the emergence of pathogens in non-endemic areas, making them an emerging global threat. This thesis work focuses on schistosomiasis, the second human parasitic disease after malaria and its recent emergence in Europe (Corsica, France). After the identification and genomic characterization of a hybrid parasite between two distinct agents of the disease, S. haematobium in humans and S. bovis in cattle, we conducted an integrative approach to characterize at several scales the invasive capacities and virulence of such parasites. Starting from the field, we set up an experimental evolution protocol aimed at generating first- and second-generation hybrids in the laboratory. We analysed life history trait modifications of these parasites as well as the molecular consequences (genomics and transcriptomics) of this "genomic clash" and we show that hybridization can be a major evolutionary force for parasites.

Un développement rapide et une densification du trafic aérien ont conduit à l'introduction de nouvelles opérations d'approches et d'atterrissage utilisant des trajectoires plus flexibles et des minimas plus exigeants. La plupart des opérations de navigation aérienne sont actuellement réalisées grâce au GNSS, augmenté par les systèmes GBAS, SBAS et ABAS qui permettent d'atteindre des opérations d'approches de précision (pour GBAS et SBAS). Cependant ces systèmes nécessitent la mise en place d'un réseau de station de référence relativement couteux et des diffusions constantes de messages aux utilisateurs de l'espace aérien. Afin de surmonter ces contraintes, le système ABAS intègre à bord des informations fournies par les systèmes de navigation inertielle (INS) ainsi améliorant les performances de navigation. Dans cette logique, les avions commerciaux actuels utilisent une solution de couplage des deux systèmes appelée hybridation GPS/baro-INS. Cette solution permet d'atteindre des niveaux de performance en termes de précision, intégrité, disponibilité et continuité supérieurs aux deux systèmes pris séparément. Malheureusement, les niveaux d'exigences requis par les opérations de précision ou les atterrissages automatiques ne peuvent pas encore être totalement couverts par les solutions d'hybridation actuelles. L'idée principale de cette thèse a été d'étendre le processus d'hybridation en incluant d'autres capteurs ou systèmes actuellement disponibles ou non à bord et d'évaluer les niveaux de performance atteints par cette solution de filtre d'hybridation global. L'objectif ciblé est de pouvoir fournir la plupart des paramètres de navigations pour les opérations les plus critiques avec le niveau de performance requis par les exigences OACI. Les opérations ciblées pendant l'étude étaient les approches de précision (en particulier les approches CAT III) et le roulage sur la piste. L'étude des systèmes vidéo a fait l'objet d'une attention particulière pendant la thèse. La navigation basée sur la vidéo est une solution autonome de navigation de plus en plus utilisée de nos jours axée sur des capteurs qui mesurent le mouvement du véhicule et observent l'environnement. Que cela soit pour compenser la perte ou la dégradation d'un des systèmes de navigation ou pour améliorer la solution existante, les intérêts de l'utilisation de la vidéo sont nombreux. / The quick development of air traffic has led to the improvement of approach and landing operations by using flexible flight paths and by decreasing the minima required to perform these operations. Most of the aircraft operations are supported by the GNSS augmented with GBAS, SBAS and ABAS. SBAS or GBAS allow supporting navigation operations down to precision approaches. However, these augmentations do require an expensive network of reference receivers and real-time broadcast to the airborne user. To overcome, the ABAS system integrates on-board information provided by an INS so as to enhance the performance of the navigation system. In that scheme, INS is coupled with a GPS receiver in a GPS/baro-INS hybridization solution that is already performed on current commercial aircraft. This solution allows reaching better performance in terms of accuracy, integrity, availability and continuity than the two separated solutions. However the most stringent requirements for precision approaches or automatic landings cannot be fulfilled with the current hybridization. The main idea of this PhD study is then to extend the hybridization process by including other sensors already available on commercial aircraft or not and, to assess the performance reached by a global hybridization architecture. It aims at providing most of the navigation parameters in all operations with the required level of performance. The operations targeted by this hybridization are precision approaches, with a particular focus on CAT III precision approach and roll out on the runway. The study of video sensor has been particularly focused on in the thesis. Indeed video based navigation is a complete autonomous navigation opportunity only based on sensors that provide information from the dynamic of the vehicle and from the observation of the scenery. Moreover, from a possible compensation of any loss or degradation of a navigation system to the improvement of the navigation solution during the most critical operations, the interests of video are numerous.

A riqueza e a diversidade de espécies forrageiras dos Campos Sulinos, pode propiciar a inserção de espécies nativas em programas de melhoramento. As gramíneas nativas do gênero Paspalum possuem grande variabilidade, que pode contribuir para seleção de genótipos adaptáveis às várias condições ecológicas de regiões tropicais e subtropicais. Dentre as espécies deste gênero destaca-se o Paspalum notatum com ecótipos que possuem superioridade produtiva quando comparados à cultivares comerciais, o que contribui para que esta espécie seja candidata ao lançamento de novas cultivares. Avaliações de plantas melhoradas submetidas a diferentes práticas de manejo, como fertilização ou consorciação, são fundamentais para gerar conhecimento acerca das características produtivas. O objetivo deste trabalho foi avaliar o potencial produtivo de uma progênie híbrida intraespecífica de P. notatum submetida a diferentes níveis de fertilização nitrogenada ou consorciação com leguminosas para serem empregados em sistemas de produção a pasto ou na recuperação de pastagens naturais degradadas. A produção de matéria seca e a densidade populacional de perfilhos dos genótipos de P. notatum respondem de forma positiva aos níveis de fertilização nitrogenada. O nível de fertilização 120 kg N ha-1ano-1 promove a maior eficiência de uso de nitrogênio nos genótipos de P. notatum. Os genótipos B26, C22, C9 e Bagual são indicados para serem utilizados em sistemas de consórcio com leguminosas de clima temperado. A produção de matéria seca de sistemas com genótipos de P. notatum consorciados com trevo branco mais cornichão é semelhante à produção de sistemas fertilizados com até 240 kg N ha-1ano-1, evidenciando a viabilidade do consórcio entre essas espécies. / The richness and diversity of forage species in the Campos Sulinos region may facilitate the inclusion of native species in breeding programs. Native grasses of the Paspalum genus show great variability which may contribute to the selection of adaptable genotypes to the various ecological conditions of tropical and subtropical regions. Within the genus, Paspalum notatum is a species with superiorly productive ecotypes compared to commercial cultivars, rendering this species a candidate for the release of new cultivars. The evaluation of bred plants submitted to different management practices, such as fertilization or consortium with legumes is fundamental to acquire knowledge about the productive features. The objective of the present work was to evaluate the productive potential of an intraspecific hybrid progeny of Paspalum notatum submitted to different nitrogen fertilization levels or intercropped with legumes, with the objective of being used in grazing systems or in the recovery of degraded natural pastures. The dry matter yield and tiller population density of Paspalum notatum genotypes respond positively to nitrogen fertilization levels. The 120 kg N ha-1year-1 fertilization level promotes the highest nitrogen utilization efficiency in Paspalum notatum. Genotypes B26, C22, C9 and Bagual are indicated for legume intercropped systems with temperate legumes. The dry matter production of intercropped systems between Paspalum notatum genotypes with white clover plus birdsfoot trefoil is similar to the production of systems fertilized with up to 240 kg N ha-1year-1, demonstrating the viability of the intercropped between these species.

Bromeliaceae é uma família importante entre as monocotiledôneas devido ao seu elevado número de espécies distribuídas no Neotrópico, e por uma riqueza de caracteres adaptativos a diferentes habitats. Flores de bromélias possuem uma grande variação morfológica, frequentemente negligenciada em estudos de morfoanatomia e de filogenia. Para uma melhor compreensão dos mecanismos de desenvolvimento que levam a essas variações, foram analisados aspectos moleculares e evolutivos do fator de transcrição MADS-box PISTILLATA (PI), a partir de sequências de transcritos isolados de inflorescências de bromélias nativas brasileiras. Sequências PI de Bromeliaceae foram comparadas com outras monocotiledôneas, com verificação de expressão em inflorescências de Tillandsia aeranthos (Loisel.) LB Sm. com o uso de hibridação in situ. Sequências de PI mostraram alta conservação, inclusive em um sítio de deleção encontrado para todos os membros analisados da família. Todos os membros da família se agruparam em um único clado em reconstruções filogenéticas. Entretanto, devido a características de taxas de mutações rápidas e divergência antiga do gene, não foi possível obter uma relação precisa entre diferentes famílias ou ordens. Todavia, PI mostrou ter potencial como ferramenta de análise filogenética para espécies proximamente relacionadas. Os transcritos de PI foram localizados principalmente em tecidos meristemáticos de regiões menos desenvolvidas de inflorescências de Tillandsia aeranthos. Flores mais desenvolvidas presentes nas inflorescências mostraram um padrão de acúmulo preferencial de transcritos PI em pétalas e sépalas, assim como esperado para flores com perianto diferenciado em sépalas e pétalas. De qualquer forma, Tillandsia aeranthos se mostrou eficiente para estudos morfoanatômicos com enfoque em desenvolvimento evolutivo. / Bromeliaceae is an important monocotyledon family due to its high number of species in the Neotropic, and a wealth of adaptive characters to different habitats. Bromeliad flowers have large morphological variations, often neglected in morpho-anatomy, floral development and phylogeny studies. For a better understanding of its floral developmental mechanisms that lead to morphological variations, molecular and evolutionary aspects of the transcription factor MADS-box PISTILLATA (PI) encoding gene isolated had been investigated in native bromeliad inflorescences. Bromeliaceae PI sequences were compared with other monocots, and the expression of these transcripts was detected in Tillandsia aeranthos (Loisel.) LB Sm. inflorescences using in situ hybridization. The PI sequences display high conservation, including a specific deletion site found in all family members. Likewise, all Bromeliaceae members grouped into a single clade in phylogeny reconstruction. However, due to rapid mutation rate and ancient divergence in PI, it was not possible to obtain a precise relationship between different monocot families and orders. Nevertheless, PI showed potential as a phylogenetic tool for analysis between closely related species. PI transcripts were located mainly in meristematic tissues from less developed regions of the inflorescences. More developed flowers showed a preferential PI transcripts accumulation in petals and stamens as expected for differentiated perianth, found in Bromeliaceae flowers. Anyway, Tillandsia aeranthos showed good potential skills for morpho-anatomy focused in evolutionary development.

Submitted by VICTOR BERNARDES BARROSO DA COSTA null (victorbernardes_@hotmail.com) on 2016-03-16T04:08:27Z No. of bitstreams: 1 Dissertação - VICTOR (Versão Final).pdf: 20193346 bytes, checksum: f7da64e79358575f01cf61a5054c4587 (MD5) / Approved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2016-03-18T12:52:04Z (GMT) No. of bitstreams: 1 costa_vbb_me_sjc.pdf: 20193346 bytes, checksum: f7da64e79358575f01cf61a5054c4587 (MD5) / Made available in DSpace on 2016-03-18T12:52:04Z (GMT). No. of bitstreams: 1 costa_vbb_me_sjc.pdf: 20193346 bytes, checksum: f7da64e79358575f01cf61a5054c4587 (MD5) Previous issue date: 2016-01-21 / Submitted by VICTOR BERNARDES BARROSO DA COSTA null (victorbernardes_@hotmail.com) on 2016-01-26T01:08:29Z No. of bitstreams: 1 Dissertação - VICTOR (Versão Final).pdf: 20193437 bytes, checksum: c041b8e5fa02ed4f74d445c5838128fd (MD5) / Approved for entry into archive by Sandra Manzano de Almeida (smanzano@marilia.unesp.br) on 2016-01-26T13:39:40Z (GMT) No. of bitstreams: 1 costa_vbb_me_sjc.pdf: 20193437 bytes, checksum: c041b8e5fa02ed4f74d445c5838128fd (MD5) / Made available in DSpace on 2016-01-26T13:39:40Z (GMT). No. of bitstreams: 1 costa_vbb_me_sjc.pdf: 20193437 bytes, checksum: c041b8e5fa02ed4f74d445c5838128fd (MD5) Previous issue date: 2016-01-21 / Item merged in doublecheck by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-03-21T13:12:44Z Item was identical to item(s): 135509, 132061 at handle(s): http://hdl.handle.net/11449/136305, http://hdl.handle.net/11449/132945 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) / O carcinoma de células escamosas (CEC) de boca é uma neoplasia incomum em pacientes jovens. Na literatura de língua inglesa não há relatos de estudos que investiguem a amplificação do EGFR e a expressão desta proteína neste grupo etário. O objetivo deste estudo foi investigar a amplificação do EGFR através da técnica de hibridização por fluorescência in situ (FISH) e correlacionar os resultados obtidos através da imunomarcação da proteína EGFR com a epidemiologia e com o prognóstico dos pacientes avaliados. Ao final dos testes de FISH e imuno- histoquímicos, 21 amostras do grupo teste (pacientes ≤ 40 anos) e 39 amostras do grupo controle (pacientes ≥ 50 anos) foram consideradas adequadas para avaliação. As variáveis clínicas e anatomopatológicas foram comparadas pelos testes Qui-Quadrado ou exato de Fisher. A expressão do marcador EGFR e do método de FISH foi comparada entre os grupos por meio do teste não paramétrico de Mann-Whitney. As curvas de sobrevida foram calculadas utilizando o método de Kaplan-Meier e suas curvas foram comparadas através do teste de log-rank. Houve maior número de pacientes do sexo masculino, leucodermas, tabagistas e etilistas. A amplificação do EGFR foi maior no grupo teste (p = 0,018). A amplificação do EGFR associou-se estatisticamente com a variável estadiamento clínico avançado (p = 0,013), independente do grupo. A expressão da proteína EGFR correlacionou-se com tumores bem diferenciados (p = 0,011) e a presença de metástase (p = 0,035), independente da idade. A presença de amplificação foi mais frequente no grupo tese. Alguns casos de pacientes ≥40 anos de idade podem ser adequados ao emprego da terapia anti-EGFR, devido à amplificação do EGFR. / Oral squamous cell carcinoma (OSCC) is uncommon neoplasia in young patients. In the English literature, there are no reports of studies that investigate the amplification of EGFR and expression of this protein in this age group. The aim of this study was to investigate the amplification of EGFR by fluorescence in situ hybridization (FISH) and to correlate the results by immunostaining of EGFR protein with clinicopathological features and prognosis. After FISH and immunohistochemistry, 21 samples of the test group (≤ 40 years) and 39 samples of control group (≥ 50 years) were suitable for evaluating. Categorical variables were compared by the Pearson chi-square test or Fisher's exact test. Associations between protein levels and FISH results with clinicopathological characteristics of the patients were analyzed by Mann-Whitney U test. Survival rates were calculated using the Kaplan-Meier method and the curves were compared by the log-rank test. There was predominance for male patients, leucoderma, smoking and alcohol consumption. The EGFR amplification was higher in the test group (p = 0.018) and it was associated statistically with advanced clinical stage (p = 0.013), independent of the group. The expression of EGFR protein was correlated to well differentiated tumors (p = 0.011) and presence of metastasis (p = 0.035), regardless of age. Presence of EGFR amplification and/or expression. Some cases of patients ≥40 years old might be suitable for anti-EGFR therapy because of EGFR amplification. / FAPEAM: 254/2014

Submitted by Valquíria Barbieri (kikibarbi@hotmail.com) on 2017-06-20T21:26:27Z No. of bitstreams: 1 DISS_2014_Soraya do Lago Albuquerque.pdf: 1446111 bytes, checksum: cdf2c1d720b9a3bba7d2ac186acae29b (MD5) / Approved for entry into archive by Jordan (jordanbiblio@gmail.com) on 2017-06-23T16:07:15Z (GMT) No. of bitstreams: 1 DISS_2014_Soraya do Lago Albuquerque.pdf: 1446111 bytes, checksum: cdf2c1d720b9a3bba7d2ac186acae29b (MD5) / Made available in DSpace on 2017-06-23T16:07:15Z (GMT). No. of bitstreams: 1 DISS_2014_Soraya do Lago Albuquerque.pdf: 1446111 bytes, checksum: cdf2c1d720b9a3bba7d2ac186acae29b (MD5) Previous issue date: 2014-03-28 / As literaturas pós-coloniais são caracterizadas por conceder voz aos marginalizados, aos considerados subalternos e minorias, para que esses, através das múltiplas vozes, expressas pela escrita literária possam se fazer ouvir e assim buscar a tão sonhada autonomia e significação enquanto sujeito histórico. É nesse contexto que estão inseridas as duas escritoras que são foco deste estudo que traçará por um viés comparativo um estudo entre obras de duas escritoras de países africanos: Paulina Chiziane, moçambicana e Toni Morrison, estadunidense e afrodescendente. Ambas expressam por meio da literatura os costumes de seu povo, desencadeando por meio das memórias um fluxo narrativo pelos rastros deixados pela oratura. A inserção da mulher no mundo literário foi uma das inovações das literaturas pós-coloniais dos países africanos e afrodescendentes, pois até então a mulher não expressava suas necessidades e muito menos indignações, notadamente pela construção social, política e histórica da sociedade patriarcal e sexista. Com a subjugação colonial muito menos ainda poderia ser ouvida. Ao contrário, as emoções foram silenciadas em seus recônditos, aguardando o instante para verter em emponderamento a voz na literatura e, em ação, as atividades políticas feministas. Acreditando nesse empoderamento dado à literatura pretendemos mostrar como as duas escritoras apropriam as suas personagens com ele e traça o diálogo com propostas de mudança e de subversão ao cenário no qual se encontram. / Postcolonial literatures are characterized by granting voice to the marginalized , who are considered subaltern and the minorities for those, through the multiple voices which are expressed by literary writing can be heard and so it can be able to find so awaited autonomy and significance as a historical human being . It is in this context that the two writers are inserted in which they are the focus of this study which intend to do a comparative study between the works of the two writers of African countries : Paulina Chiziane , moçambiquean and Toni Morrison , American and African descent . Both express through the literature the customs of their people , triggering memories through a narrative flow through the traces left by the orature . The women1s inclusion in the literary world was one of the innovations of postcolonial literatures of African countries and African descent ones because until there the woman neither express their needs nor their outrage , especially by the social building , political and historical of the patriarchal and sexist society. With the colonial subjugation it couldn’t be be heard at all. Instead of this, the emotions were muted in their secluded , waiting for the moment to change it into empowerment the voice in literature and, in action, the feminist political activities . Trusting in that empowerment which was given to the literature we intend to show how the two writers appropriated their characters with it and we also goal to get the dialogue with the changing proposals and the subversion of the setting in which they find themselves.