L’exercice fractionné de haute intensité (HIIT) et continu d’intensité modéré (MOD) représentent les 2 grandes modalités d'endurance. Cependant, leur impact spécifique sur la performance physique et la plasticité cérébrale et musculaire reste controversé du fait de la diversité des protocoles d’exercice proposés chez les sédentaires. Notre 1er axe dégage chez des rats sains l’effet de ces 2 modalités sur la performance physique, la plasticité musculaire et cérébrale sur 8 semaines d’entrainement standardisée dont l'intensité est basées sur le seuil lactique. Nos résultats montrent des gains de performance d’endurance plus rapides et importants suite aux HIIT. La neuroplasticité serait stimulée par les HIIT uniquement et la plasticité musculaire semble spécifique à chacune. L’engouement pour les HIIT se répercute chez les patients subissant un accident vasculaire cérébral où l’efficacité des méthodes d’endurance n’est pas clairement justifiées. Une étude antérieure a montré l’intérêt majeur des HIIT dans la phase aiguë de la pathologie malgré une récupération sensorimotrice incomplète. D’où l’intérêt d’approfondir dans notre second axe l’effet des différentes formes de HIIT (longs et courts) dans la récupération. Nos résultats montrent principalement que ces deux formes de HIIT améliorent la capacité d’endurance et la force de la patte antérieure lésée avec une précocité pour les HIIT longs. Les deux formes de HIIT semblent induire une angiogenèse cérébrale. Néanmoins, ils n’améliorent pas les fonctions sensorimotrices et cognitives. Ainsi, il est nécessaire d’approfondir les répercussions de ces deux entrainements HIIT dans la plasticité musculaire et cérébrale. / Endurance exercise is essential for different reasons in athlete and also in aging and pathological people. Two training modalities were found: high intensity interval training (HIIT) and moderate intensity aerobic training (MOD). However, the specific outcomes of these modalities on physical performance and cerebral and muscular plasticity are controversial because many exercise protocols exist. The 1st study explore the impact of these 2 training on endurance and functional capacity and also on muscular and cerebral molecular modifications throughout 8 weeks in healthy rats. HIIT and MOD programs are work-matched and training intensity are determined thanks to the lactate threshold. Our results show a superior and fast effect on endurance capacity after HIIT compared to MOD. Hippocampal plasticity is stimulated only after HIIT and muscular modifications appear to be specific to each modality. A great interest of HIIT is found in stroke patients for whom evidence of endurance modalities efficiency is still missing. A previous study has shown a beneficial effect of HIIT in the acute phase of stroke despite incomplete sensorimotor recuperation. Hence, the interest to deepen in second part of this manuscript the impact of two HIIT modalities (short and long) in recovery optimisation. Our results show that 2 HIIT strongly improve endurance performance and strength of injured paw with a fast effect for long HIIT. The 2 modalities seem to induce cerebral angiogenesis. However, these 2 training do not increase sensorimotor and cognitive functions. In perspective, it appears necessary to develop muscular and cerebral outcomes induced by these 2 HIIT modalities.

<p>The overall aims of the studies were to investigate the effects of different training modalities on exercise capacity and health-related quality of life (HRQoL) in patients with moderate or severe COPD and, further, to explore two of the physical tests used in pulmonary rehabilitation.</p><p>In <b>study I</b>, the 12-minute walking distance (12MWD) did not increase on retesting in patients with exercise-induced hypoxemia (EIH) whereas 12MWD increased significantly on retesting in the non-EIH patients. In <b>study II</b>, we found that the incremental shuttle walking test was as good a predictor of peak exercise capacity (W peak) as peak oxygen uptake (VO₂ peak) is. In <b>study III</b>, we investigated the effects of two different combination training programmes when training twice a week for eight weeks. One programme was mainly based on endurance training (group A) and the other on resistance training and callisthenics (group B). W peak and 12MWD increased in group A but not in group B. HRQoL, anxiety and depression were unchanged in both groups. Ratings of perceived exertion at rest were significantly lower in group A than in group B after training and during 12 months of follow-up. Twelve months post-training, 12MWD was back to baseline in group A, but significantly shorter than at baseline in group B. Thus, a short endurance training intervention delayed decline in 12MWD for at least one year. Patients with moderate and severe COPD responded to training in the same way. In <b>study IV</b>, both interval and continuous endurance training increased W peak, VO₂ peak, peak exhaled carbon dioxide (VCO₂ peak) and 12MWD. Likewise, HRQoL, dyspnoea during activities of daily life, anxiety and depression improved similarly in both groups. At a fixed, submaximal workload (isotime), the interval training reduced oxygen cost and ventilatory demand significantly more than the continuous training did.</p>

The overall aims of the studies were to investigate the effects of different training modalities on exercise capacity and health-related quality of life (HRQoL) in patients with moderate or severe COPD and, further, to explore two of the physical tests used in pulmonary rehabilitation. In study I, the 12-minute walking distance (12MWD) did not increase on retesting in patients with exercise-induced hypoxemia (EIH) whereas 12MWD increased significantly on retesting in the non-EIH patients. In study II, we found that the incremental shuttle walking test was as good a predictor of peak exercise capacity (W peak) as peak oxygen uptake (VO2 peak) is. In study III, we investigated the effects of two different combination training programmes when training twice a week for eight weeks. One programme was mainly based on endurance training (group A) and the other on resistance training and callisthenics (group B). W peak and 12MWD increased in group A but not in group B. HRQoL, anxiety and depression were unchanged in both groups. Ratings of perceived exertion at rest were significantly lower in group A than in group B after training and during 12 months of follow-up. Twelve months post-training, 12MWD was back to baseline in group A, but significantly shorter than at baseline in group B. Thus, a short endurance training intervention delayed decline in 12MWD for at least one year. Patients with moderate and severe COPD responded to training in the same way. In study IV, both interval and continuous endurance training increased W peak, VO2 peak, peak exhaled carbon dioxide (VCO2 peak) and 12MWD. Likewise, HRQoL, dyspnoea during activities of daily life, anxiety and depression improved similarly in both groups. At a fixed, submaximal workload (isotime), the interval training reduced oxygen cost and ventilatory demand significantly more than the continuous training did.

La finalitat d’aquest estudi és, des d’una perspectiva fisiològica, descriure i comparar la resposta aguda a dues sessions d’entrenament de càrrega global moderada però de diferent intensitat i distribució de l’esforç. Els participants, 9 homes i 1 dona amb bona condició física, varen realitzar dues sessions d’entrenament que varen consistir en 45 minuts de cursa contínua al 90% de la velocitat del llindar de lactat individual (vIAT), o 8 sèries de 4 minuts de cursa al 105% de la vIAT i pauses de 2’30” (sessió CI). Abans i durant la recuperació es va procedir a l’extracció de sang venosa per a l’anàlisi dels paràmetres estudiats. Els resultats indiquen que l’exercici físic aeròbic en intervals causa una resposta pro-inflamatòria i pro-oxidant superior a la de l’exercici físic continu, però aquesta és de poca magnitud i transitòria. També s’ha apreciat que les concentracions plasmàtiques de lactat, IL-8 i d’urat augmenten més si la intensitat de l’esforç és elevada; mentre que les de IL-6 i IL-10 s’eleven en major mesura després de l’exercici físic continuo. Per altra banda, l’anàlisi del metaboloma plasmàtic suggereix que els canvis estan dominats per alteracions de compostos lipídics. En general els canvis varen ser moderats i transitoris, i no sembla que l’exercici aeròbic en intervals de durada mitjana suposi un risc elevat per a persones habituades a realitzar exercici físic. / La finalidad del estudio es, desde una perspectiva fisiológica, describir y comparar la respuesta aguda a dos sesiones de entrenamiento de carga global moderada pero de diferente intensidad y distribución del esfuerzo. Los participantes, 9 varones y 1 mujer con buena condición física realizaron dos sesiones de entrenamiento que consistieron en 45 minutos de carrera continua al 90% de la velocidad del umbral de lactato individual (vIAT), o 8 series de 4 minutos de carrera al 105% de la vIAT y pausas de 2’30”. Antes y durante la recuperación se procedió a la extracción de sangre venosa para el análisis de los parámetros estudiados. Los resultados indican que el ejercicio físico aeróbico en intervalos causa una respuesta pro-inflamatoria y pro-oxidante superior a la del ejercicio físico continuo de intensidad moderada, pero de poca magnitud y transitoria. També se ha apreciado que las concentraciones plasmáticas de lactato, IL-8 y urato aumentan más si la intensidad del esfuerzo es elevada; mientras que las de IL-6 y IL-10 crecen más después del ejercicio físico continuo. Por otro lado, el análisis del metaboloma plasmático sugiere que los cambios inducidos por el ejercicio están dominados por alteraciones de compuestos lipídicos. En general las alteraciones fueron moderadas y transitorias y no parece que el ejercicio aeróbico en intervalos suponga un riesgo elevado para las personas acostumbradas a realizar ejercicio físico regular. / The aim of the study was, from a physiological perspective, to describe and to compare the acute response to two training sessions of moderate global load, but of different intensity and distribution of the exertion. Participants, 9 men and one woman with good physical condition, performed two training sessions that consisted in 45 minutes of continuous running at the 90% of individual anaerobic threshold speed, or 8 series of 5 minutes at the 105% of individual anaerobic threshold speed with rest pauses of 2’30” between them. Before and during the recovery period venous blood samples were drawn for the analysis of hematologic and biochemistry parameters. Results show that aerobic interval training causes more pro-inflammatory and pro-oxidant response compared to continuous running at moderate intensity, but changes are moderate and transient. In the same way, lactate, IL-8 and urate plasma concentrations were superior after high intensity exercise, meanwhile IL-6 and IL-10 plasma concentrations were more respondents to continuous running and to the greater duration of the physical work. On the other hand, the non-oriented metabolomic approach suggests that changes in plasma metabolome induced by the exercise are dominated by lipid molecular species. Altogether changes were moderate and transient, and it seems that aerobic interval training does not represent an important risk for trained individuals.

This thesis examined the translocation of fatty acid (FA) transport proteins to the plasma membrane (PM) in human and rat skeletal muscle during moderate intensity exercise. The responses to the post-exercise period and to acute moderate intensity exercise after 6 weeks of high intensity interval training (HIIT) were also examined in humans. The overall hypotheses were that 1) FAT/CD36 and FABPpm would translocate to the PM in human skeletal muscle during 120 min of moderate intensity exercise, 2) FAT/CD36 and FABPpm would translocate to the PM in rat skeletal muscle during 120 min of moderate intensity exercise and this would correlate to an increase in palmitate uptake, 3) FAT/CD36 and FABPpm would translocate to the PM during 120 min of moderate intensity exercise, but return to basal levels by 45 min post-exercise, 4) six weeks of HIIT would increase PM content of FABPpm but not FAT/CD36 in resting skeletal muscle, 5) six weeks of HIIT would cause a further increase in the translocation of FAT/CD36 and FABPpm to the PM during moderate intensity exercise and this would correspond to an increase in whole body fat oxidation compared to exercise pre-training, and 6) six weeks of HIIT would increase whole muscle content of FATP1 and FATP4. In human skeletal muscle, PM FAT/CD36 and FABPpm increased 75% and 20% respectively after 120 min of cycling at ~60% VO2 peak which corresponded to a 110% increase in whole body fat oxidation. In rat skeletal muscle, PM FAT/CD36 and FABPpm increased 20% and 30% respectively, which correlated to a 30% increase in palmitate uptake following 120 min of treadmill running at ~65% VO2 peak. The PM content of FAT/CD36 increased further to 120% of resting values by 45 min of post-exercise following 120 min of cycling at ~60% VO2peak, which correlated with a heavy reliance on fat as a fuel during the post-exercise period. FABPpm returned to resting levels of PM content by 15 min post-exercise. After 6 wk of HIIT, whole muscle FAT/CD36 (50%), FABPpm (21%) and FATP4 (25%) were increased in human skeletal muscle, while FATP1 remained unchanged. There were no changes in PM content of FAT/CD36 or FABPpm at rest following training. FAT/CD36 and FABPpm were also measured before and after 120 min of cycling at ~60% of pre-trainingVO2 peak following training, but no differences in the magnitude of the PM content increases were seen compared to pre-training, despite a 27% increase in fat oxidation. These studies demonstrate that FA transport proteins translocate to the PM during moderate intensity exercise, which correlates with increased FA uptake and whole body fat oxidation. This relationship does not appear to hold during the post-exercise period, as further increases in the PM content of FAT/CD36 does not correspond with the decrease in fat oxidation. The PM content of FAT/CD36 and FABPpm were not increased at rest following training, and there was no effect of training on the translocation of FAT/CD36 or FABPpm to the PM during moderate intensity exercise at the same absolute power output, however there may be a further increase at a relative power output. / Natural Sciences and Engineering Research Council, Canadian Institute of Health Research, Ontario Graduate Scholarship

Made available in DSpace on 2014-06-11T19:22:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-25Bitstream added on 2014-06-13T19:49:12Z : No. of bitstreams: 1 barbosa_lf_me_rcla.pdf: 370403 bytes, checksum: 01d2e0d380cd68aad350f8cb36b5f132 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo deste estudo foi verificar a validade da máxima fase estável de lactato (MLSS) como fronteira entre o domínio pesado e severo, analisar o efeito do tempo para análise cinética da concentração de lactato sanguíneo ([La]) sobre a (MLSSw) e a [La] correspondentes a MLSS em exercícios intermitentes (MLSSi) com recuperação passiva e ativa e, verificar a influência da recuperação passiva ou ativa na [La], MLSSw, freqüência cardíaca (FC) e intensidade relativa à potência máxima (%Wmax) correspondentes à MLSS. Dezenove ciclistas treinados do gênero masculino divididos em dois grupos, recuperação passiva (VO2max = 3960,6 ± 312,8 ml.min-1; N = 9) ou ativa (VO2max = 3958,3 ± 318,3 ml.min-1; N = 10), realizaram os seguintes testes, em dias diferentes, em um cicloergômetro: 1) Teste incremental até a exaustão; 2) 2 a 3 testes de carga contínua para a determinação da MLSS de forma contínua (MLSSc) e; 3) testes intermitentes de carga submáxima (8 x 4 min, com 2 min de recuperação passiva ou ativa) para determinar a MLSSi com recuperação passiva (MLSSip) e ativa (MLSSia). A MLSSi foi definida como a mais alta carga na qual a [La] não aumentou mais do que 1 mM entre o 10o e o 30o min (critério 1 – C1) ou 14o e 44o min (critério 2 – C2) do protocolo. Os valores do VO2 obtidos no 30o min de exercício foi estatisticamente maior que o valor obtido no 3o min de exercício a 100%MLSSc (3379,3 ± 250,1 ml/min vs. 3496,7 ± 280,2 ml/min, p<0,05) e a 105%MLSSc (3439,5 ± 289,3 ml/min vs. 3545,5 ± 303,2 ml/min, p<0,05), sendo estes valores significantemente menores do que o VO2pico (3978,6 ± 296,2 ml/min, p>0,05). O tempo (C1 ou C2) parece não influenciar significantemente a [La] (Passiva – 5,89 ± 1,41 mM vs. 5,61 ± 1,78 mM; Ativa - 5,62 ± 1,83 mM vs. 4,91 ± 1,91 mM) e a MLSSw (Passiva – 294,5 ± 31,8 W vs. 294,7 ± 32,2 W; Ativa... / The aim of this study was to analyze the validity of the maximal lactate state (MLSS) as a boundary between heavy and severe domain, to check the effect of time for the analysis of blood lactate concentration ([La]) kinetics on workload (MLSSw) and related to MLSS during intermittent exercises (MLSSi) with passive and active recovery and to verify the influence of passive or active recovery at [La], MLSSw, heart rate and relative intensity of Wmax (%Wmax) correspondent to maximal lactate steady state. Nineteen male trained cyclists were divided into two groups: passive recovery (VO2max = 3960,6 ± 312,8 ml.min-1; N = 9) and active (VO2max = 3958,3 ± 318,3 ml.min-1; N = 10), they performed the following tests, in different days, on a cycle ergometer: 1) Incremental test until exhaustion and; 2) 2 to 3 constants workload tests to determine (MLSSc) and; 3) intermittent submaximal workload tests (8 x 4 min, with 2 min of passive or active recovery) to determine MLSSi with passive recovery (MLSSip) and active (MLSSia). MLSSi was defined as the highest workload at which [La] increased no more than 1 mM between 10th and 30th min (criterion 1 – C1) or 14o and 44o min (criterion 2 – C2) of the protocol. VO2 values obtained at 30th min was statistically higher than that obtained at 3rd min of the exercise performed at 100%MLSS (3379.3 ± 250.1 vs. 3496.7 ± 280.2 ml/min, p<0.05) and 105%MLSS (3439.5 ± 289.3 vs. 3545.5 ± 303.2 ml/min, p<0.05) and the VO2 value obtained at 30th min of the exercise performed at 100%MLSS and 105%MLSS was statistically lower than VO2peak (3978.6 ± 296.2 ml/min, p>0.05). Time (C1 or C2) seems not influence significantly [La] (Passive – 5,89 ± 1,41 mM vs. 5,61 ± 1,78 mM; Active- 5,62 ± 1,83 mM vs. 4,91 ± 1,91 mM) and MLSSw ((Passive - 294.5 ± 31.8 vs. 294.7 ± 32.2 W; Active - 304.6 ± 23.0 vs. 300.5 ± 23.9 W). Interruptions at exercise... (Complete abstract click electronic access below)

Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2018-05-03T14:05:56Z No. of bitstreams: 2 Dissertação - Marco Antonio Basso Filho - 2018.pdf: 1179214 bytes, checksum: c42f7002852698e32ce9c89e0baff62d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-05-03T15:41:54Z (GMT) No. of bitstreams: 2 Dissertação - Marco Antonio Basso Filho - 2018.pdf: 1179214 bytes, checksum: c42f7002852698e32ce9c89e0baff62d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-05-03T15:41:54Z (GMT). No. of bitstreams: 2 Dissertação - Marco Antonio Basso Filho - 2018.pdf: 1179214 bytes, checksum: c42f7002852698e32ce9c89e0baff62d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Introduction: Studies show that high intensity interval training (HIIT) promotes improvements equivalent or even superior to continuous moderate intensity training (IMT). However, HIIT has divergent applications, approaches and results in the literature regarding its physiological repercussions and the parameters that may bring more safety in its prescription. In this context kinetics and heart rate variability (HRV) represent the physiological responses of the autonomic nervous system (ANS) in cardiac autonomic heart rate (HR) modulation, as well as VO2max represents the aerobic functional capacity and, in the rest-exercise transitions and exercise-recovery, can demonstrate the efficiency of different physical training in fitness, adaptation, vagal withdrawal and sympathetic hyperactivity. Objective: To compare the acute effects of different intensities of physical training on the kinetics of HR and HRV, and functional capacity in healthy physically active youngsters. Objective: To compare the acute effects of different intensities of physical training on the kinetics of HR and HRV, and functional capacity in healthy physically active youngsters. Methodology: Clinical crossover performed with 12 physically active young males. The procedure to obtain the data occurred for 4 days with an interval of 48 hours between those days. On the 1st day the participants were submitted to evaluations including the cardiopulmonary exercise test (CPT) for the purpose of verifying VO2max, identifying their aerobic functional capacity, as well as prescribing the protocols. Between the 2nd and 4th day, the three exercise protocols were performed: TCMI (exercise = 21 minutes continuous at 70% of vVO2max), short HIIT (exercise = 29 repetitions of 30 seconds at 100% of vVO2max and recovery = 30 seconds passive to 50% of vVO2max) and long HIIT (exercise = 3 replicates of 4 minutes to 90% of vVO2max and recovery = 3 minutes to 60% of vVO2max). Participants performed 5 minutes of heating at 55% of vVO2max and at the end of the session 3 minutes of recovery at 50% of vVO2max. The recording data of the R-R intervals were recorded with the participant lying in the supine position for 10 minutes before the start of the tests and immediately after the recovery of the sit-in tests for 3 minutes. The behavior of the HR, HRV and VO2max variables were verified in the rest-exercise transition, during exercise and in the exercise-recovery transition. Statistical analysis: The normality of the data was evaluated by the Komolgorov-Smirnov test. In order to evaluate the correlation between HRV indexes after each exercise protocol and VO2peak, the Pearson Correlation Test or the Spearman Correlation Test were used. Two-way ANOVA was used to verify the differences between the variables obtained in each exercise protocol (HRV indices and cardiorespiratory fitness variables). Values of p <0.05 were considered significant. Results: The systolic blood pressure (SBP) variable showed a lower value in the short HIIT when compared to the long HIIT (p = 0.003). The analysis of FCon kinetics showed a statistical difference for the delta time variable (∆T) when we compared the IMT and the long HIIT (p = 0.041), observing that the IMT promoted a greater slowness in the HR response in the rest-exercise transition. The analysis of the HRV during the exercise-recovery phase (off) was different between the long HIIT versus the IMT and the long HIIT versus the short HIIT (p <0.005 and p = 0.012, respectively), and the long HIIT showed greater sympathetic modulation and lower parasympathetic modulation. The comparison of the differences between the deltas of FCoff, the long HIIT showed a lower HR reduction in the initial 60 seconds of recovery when compared to the short HIIT and IMT respectively, presented by delta 30 (p <0.001 and p = 0.034, respectively) and Delta 60 (p = 0.012 and p = 0.037, respectively). The VO2peak variable had a higher value in the long HIIT when compared to the IMT and the short HIIT (p = 0.028 and p <0.001, respectively). Conclusion: In the intergroup comparison the IMT presented better HR values in the rest-exercise transition and the long HIIT obtained better results in the variables VO2peak and FCpeak. In terms of HRV in the exercise-recovery transition the short HIIT demonstrated better adaptation in the autonomic modulation while the long HIIT showed greater sympathetic hyperactivity and consequent cardiac overload. / Introdução: Estudos demonstram que o treino intervalado de alta intensidade (HIIT) promove melhorias equivalentes ou até mesmo superiores ao treinamento contínuo de moderada intensidade (TCMI). No entanto, o HIIT tem aplicações, abordagens e resultados divergentes na literatura em relação as suas repercussões fisiológicas e dos parâmetros que possam trazer mais segurança em sua prescrição. Neste contexto, a cinética e a variabilidade da frequência cardíaca (VFC) representam as respostas fisiológicas do sistema nervoso autônomo (SNA) na modulação autonômica da frequência cardíaca (FC), assim como o VO2máx representa a capacidade funcional aeróbia e, nas transições repouso-exercício e exercício- recuperação, podem demonstrar a eficiência de diferentes treinos físicos na aptidão, adaptação, retirada vagal e hiperatividade simpática. Objetivo: Comparar os efeitos agudos de diferentes intensidades de treinamento físico sobre a cinética da FC e VFC, e capacidade funcional em jovens saudáveis fisicamente ativos. Metodologia: Ensaio clínico crossover realizado com 12 jovens do sexo masculino ativos fisicamente. O procedimento para obtenção dos dados ocorreu por 4 dias com intervalo de 48 horas entre esses dias. No 1º dia os participantes foram submetidos a avaliações, entre elas o teste de esforço cardiopulmonar (TECP), com a finalidade de verificar VO2máx, identificar sua capacidade funcional aeróbia, bem como para prescrever os protocolos. Entre o 2º e o 4º dia foram realizados os três protocolos de exercícios: TCMI (exercício = 21 minutos contínuos a 70% da vVO2máx), HIIT curto (exercício = 29 repetições de 30 segundos a 100% da vVO2máxe recuperação = 30 segundos passivos a 50% da vVO2máx) e HIIT longo (exercício = 3 repetições de 4 minutos a 90% da vVO2máx e recuperação = 3 minutos a 60% da vVO2máx). Os participantes realizaram 5 minutos de aquecimento a 55% da vVO2máx e ao término da sessão 3 minutos de recuperação a 50% da vVO2máx.Os dados da gravação dos intervalos R-R foram registrados com o participante deitado em posição supina por 10 minutos antes do início dos testes e imediatamente após o término da recuperação dos testes em postura sentada por 3 minutos. O comportamento das variáveis FC, VFC e VO2máx foram verificados na transição repousoexercício, durante o exercício e na transição exercício-recuperação. Análise estatística: A normalidade dos dados foi avaliada pelo Teste de Komolgorov-Smirnov. Para avaliar a correlação entre os índices da VFC após cada protocolo de exercício e o VO2pico foram utilizados o Teste de Correlação de Pearson ou o Teste de Correlação de Spearman. A ANOVA twoway foi utilizada para verificar as diferenças entre as variáveis obtidas em cada protocolo de exercício (índices da VFC e variáveis da aptidão cardiorrespiratória). Foram considerados significativos valores de p < 0,05. Resultados: A variável pressão arterial sistólica (PAS) mostrou menor valor no HIIT curto quando comparada ao HIIT longo (p = 0.003). A análise da cinética da FCon demonstrou diferença estatística para a variável delta tempo (∆T) ao compararmos o TCMI e HIIT longo (p = 0.041), observando que o TCMI promoveu maior lentificação na resposta da FC na transição repouso-exercício. A análise da VFC durante a fase de exercício-recuperação (off) mostrou-se diferente entre o HIIT longo versus TCMI e HIIT longo versus HIIT curto (p < 0.005 e p = 0.012, respectivamente), sendo que o HIIT longo mostrou maior modulação simpática e menor modulação parassimpática. A comparação das diferenças entre os deltas da FCoff, o HIIT longo apresentou menor redução da FC nos 60 segundos iniciais da recuperação quando comparados ao HIIT curto e TCMI respectivamente, apresentado pelo delta 30 (p < 0.001 e p = 0.034, respectivamente) e Delta 60 (p = 0.012 e p = 0.037, respectivamente). A variável VO2pico apresentou maior valor no HIIT longo quando comparado ao TCMI e ao HIIT curto (p = 0.028 e p < 0.001, respectivamente). Conclusão: Na comparação intergrupos o TCMI apresentou melhores valores de FC na transição repousoexercício e o HIIT longo obteve melhores resultados nas variáveis VO2pico e FCpico. Em termos de VFC na transição exercício-recuperação, o HIIT curto demonstrou melhor adaptação na modulação autonômica enquanto o HIIT longo mostrou maior hiperatividade simpática e consequente sobrecarga cardíaca.

High intensity interval training (HIIT) has become very popular due to the dissemination of experimental results. HIIT promotes similar adaptations to longterm and low-intensity exercises, such as improvement in cardiorespiratory capacity, VO2max, respi- ratory metabolism, increased pulmonary ventilation, among others. However, these adaptations are obtained with much lower duration at maximum or submaximal intensities, with seconds or few minutes of interval. However, frequent performance may increase susceptibility to injury, promote chronic fatigue and overtraining, partly because of the high synthesis of reactive oxygen species (ROS). Stress is a state of imbalance between oxidation and oxidation reactions. However, the effects of HIIT on oxidative status and muscle damage are still not well understood in the scientific literature. The objective of this dissertation was to verify the effects of short-term HIIT on biomarkers of oxidative stress and muscle damage in Wistar rats. Thus, three studies were elaborated: 1) The effects of short-term HIIT on the markers of oxidative stress and muscle damage were evaluated; 2) It was sought to characterize the responses of cardiac oxidative stress markers to HIIT in rats. 3) The effects of twelve consecutive sessions and on different days of HIIT on the markers of hepatic oxidative stress were verified. In studies 1 and 2 significant changes were found in only one marker of oxidative damage, a fact that did not happen in study 3. Still on study 1 there were no changes in the markers of muscle damage, however there was a significant reduction in a marker of defense antioxidant. In studies 2 and 3 there were no significant changes in antioxidant capacity. It is concluded that HIIT is performed on consecutive or distinct days, and depending on tissue it may or may not promote liver, heart and muscle damage in rats. / O treinamento intervalado de alta intensidade (HIIT) na comunidade científica tem se tornado muito notório em função da divulgação de estudos experimentais. O HIIT promove adaptações semelhantes aos exercícios de longa duração e baixa intensidade, tais como melhora da capacidade cardiorrespiratória, do metabolismo de respouso, aumento da ventilação pulmonar, entre outros. Porém, estas adaptações são obtidas com duração muito inferior em intensidades máximas ou submáximas, com segundos ou poucos minutos de intervalo. Contudo, a frequente realização pode aumentar a suscetibilidade a lesões, promover a fadiga crônica e overtraining, parcialmente em razão da elevada síntese de espécies reativas de oxigênio (EROs). Estresse oxidativo é um estado de desequilíbrio entre as reações de oxidação e de antioxidação. Entretanto, ainda existem lacunas a serem preenchidas acerca dos efeitos do HIIT sobre o estado oxidativo e danos musculares, sobretudo em modelo animal. O objetivo desta dissertação foi verificar os efeitos do HIIT de curto prazo sobre os biomarcadores de estresse oxidativo e danos musculares em ratos. Desta forma, foram elaborados três estudos: 1. Avaliou-se os efeitos do HIIT de curto prazo sobre os marcadores de estresse oxidativo e danos musculares; 2. Buscou-se caracterizar as respostas dos marcadores de estresse oxidativo cardíaco ao HIIT em ratos. 3. Verificou-se os efeitos de doze sessões consecutivas e em dias distintos de HIIT sobre os marcadores de estresse oxidativo hepático. Nos estudos 1 e 2 foram encontradas alterações significativas em apenas um marcador de dano oxidativo, fato este que não aconteceu no estudo 3. Ainda sobre o estudo 1 não houveram alterações nos marcadores de danos musculares, no entanto houve uma redução significativa num marcador de defesa antioxidante. Nos estudos 2 e 3 não houveram mudanças significativas na capacidade antioxidante. Conclui-se que o HIIT, seja realizado em dias consecutivos ou distinitos, e a depender do tecido pode ou não promover danos hepáticos, cardíacos e musculares em ratos. / São Cristóvão, SE

O treinamento resistido intervalado de alta intensidade (high intensity interval resistance training-HIIRT) tem se tornado cada vez mais popular nos últimos anos poispromoveresultados positivos com curtas sessões de treinamento. No entanto, sua alta intensidade pode causar efeitos adversos. O objetivo deste estudo foi avaliar se uma sessão de HIIRT causa dano muscular agudo e alterações nos biomarcadores de lesão renal. Cinquenta e oito voluntários saudáveis, divididos igualmente entre homens e mulheres com 24 anos de idade (mediana), participaram deste estudo. Nenhum deles usou suplementos dietéticos ou medicamentos. Eles foram submetidos a cinco minutos de aquecimento seguido de quatro minutos de HIIRT. Uma escala numérica de Borg para dor (CR10P), amostras de sangue e urina foram coletadas antes (basal), 2 e 24h após a sessão HIIRT. As amostras de sangue foram analisadas e dosadocreatinina sérica (CrS),lipocalina associada a gelatinase de neutrófilos (NGALS), creatinofosfoquinase (CPK) e mioglobina (Mio). As amostras urinárias foram analisadas e dosadoscreatinina (CrU),lipocalina associada a gelatinase de neutrófilos (NGALU), interleucina 18 (IL-18), calbindina, microalbuminúria (?albumina), trefoil factor-3(TFF3) e beta-2microglobulina (ß2M). O CR10 e CPK tiveramum aumento significativo e crescente após2 e 24h. Mioaumentou significativamente em 2h e continuouelevadaapós24h. CrSaumentou significativamente após24h e em três homens,o aumento atingiu os critérios para o diagnóstico de injúria renal aguda (IRA). Todos os biomarcadores urinários aumentaram significativamente 2 horas após o exercício e retornaram aos valores basais24h após HIIRT. Concluindo, uma única sessão de HIIRT em indivíduos jovens e saudáveis causou elevações precocese significativas em CPK, mioglobina, CrS,microalbuminúria e biomarcadores urinários, indicando lesão tubularrenal, sugerindo a ocorrência de rabdomiólise e danos funcionais eestruturais aos rins / High intensity interval resistance training (HIIRT) emerged as one of the fastest growing exercise programs in recent years because provides positive results with short training sessions. However, its high intensity might cause adverse effects. The aim of this study was to evaluate if a session of HIIRT causes acute muscle damage and changes in kidney injury biomarkers. Fifty-eight healthy volunteers, divided equally among men and women (median age 24 years), participated in this study. None of them used dietary supplements or medications. They were submitted to five minutes of warm-up followed by four minutes of HIIRT. A Borg CR10 Scale for pain (CR10P), and blood and urinary samples were collected before (baseline), 2 and 24h after HIIRT session. Blood samples were analyzed for serum creatinine (SCr), neutrophil gelatinase-associated lipocalin (SNGAL), creatine kinase(CK) and myoglobin (Myo). Urinary samples were assessed for creatinine (UCr), neutrophil gelatinase-associated lipocalin (UNGAL), interleukin 18 (IL-18), calbindin, microalbuminuria (ualbumin), trefoil factor-3 (TFF3) and beta-2 microglobulin (beta2M). CR10 had a significantly and crescent increase on 2 and 24h. CK increased significantly on 2h and further in 24h. Myo increased significantly on 2h and stayed elevated at 24h. SCr increased significantly on 24h and inthree men the increase met criteria for acute kidney injury diagnosis. All the other serum and urinary kidney injury biomarkers increased significantly at 2 hours and returned to basal values at 24h after HIIRT. In conclusion, a single session of HIIRT inyoung, healthy individuals caused early and significant elevations in CK, myoglobin, SCr, microalbuminuria and urinary biomarkers indicative of kidney tubular injury, suggesting the occurrence of rhabdomyolysis and functional and structural kidney damage

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No. of bitstreams: 2 anelita.dissertacao.final.2013.12.04a.pdf: 2072554 bytes, checksum: e3cf58b92127a94864f0503af16ab9ff (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2013-08-30 / Sem bolsa / Introdução: Na prescrição do treinamento intervalado de alta intensidade (HIIT) o tempo de recuperação entre esforços é aspecto relevante. No entanto, o tipo de recuperação tem se mostrado como determinante do desempenho subsequente, e a maioria dos estudos tem indicado que a recuperação ativa (RA) tende a ser superior à passiva (RP). Especificamente quanto à RA, ela pode ser com o mesmo grupo muscular exercitado, com segmento corporal oposto ou com região do tronco. No entanto, esta última estratégia tem sido pouco investigada. Objetivo: Investigar os efeitos de dois tipos de RA nas respostas fisiológicas e no desempenho físico subsequente a treino intervalado de alta intensidade, considerando dois diferentes níveis de aptidão aeróbia. Materiais e métodos: O estudo foi desenvolvido com 25 homens, entre 18 e 35 anos, os quais foram alocados em um destes dois grupos: Alta Aptidão (AACR),Baixa Aptidão (BACR). Os envolvidos realizaram três dias de atividades. Na primeira visita foram mensurados: massa corporal, estatura, dobras cutâneas, frequência cardíaca, lactato sanguíneo e pressão sanguínea em repouso, além da realização de teste de potência máxima (Pmax) em cicloergômetro e teste de tempo limite (TLim a 120% da Pmax), quinze minutos após. A classificação em AACR ou BACR decorreu da Pmax obtida no teste progressivo em cicloergômetro, e alocação de acordo com a mediana. Nas vistas dois e três os sujeitos realizaram: i) aquecimento padronizado, ii) HIIT com dois blocos com quatro estímulos supramáximos cada (30 s a 60 rpm e com carga de 120% da Pmax, 30 s de recuperação passiva) e iii) TLim após o HIIT. Após aquecimento, entre os blocos do HIIT e antes do TLim, os envolvidos executaram um dos dois modos de recuperação ativa: recuperação ativa na bicicleta (bike) e recuperação ativa com exercícios estabilizadores do tronco (core). Na recuperação bike, pedalava-se a 30% da Pmax durante 3 min e na core, realizavamse três exercícios, com duração de 50 s cada um. Para análise dos dados, contou-se com estatística descritiva e os dados foram analisados com análise de variância de dois caminhos (nível de aptidão aeróbia e tipo de recuperação) com medidas repetidas. Assumiu-se p<0,05 como nível de significância. Resultados: Não houve diferença estatisticamente significante considerando nível de aptidão e tipo de recuperação para o TLim. Para recuperação core, BACR e AACR atingiram, respectivamente, 118±25 s e 142±62 s. Na recuperação bike, os valores foram de 110±24 s e 134±72 s. A recuperação bike proporcionou valores inferiores de lactato sanguíneo, mas apenas antes da segunda série de esforços (BACR: 3,62±0,76mmol para core e 2,99±0,90mmol para bike; AACR: 3,23±0,52mmol para core e 2,83±0,66mmol para bike, F=6,38, p=0,01). A9 recuperação core, por sua vez, diminui a frequência cardíaca de modo mais pronunciado antes da primeira e da segunda série do HIIT, bem como antes do TLim(F≥33,8, p<0,001). Conclusão: Considerando-se dois tipos de recuperação ativa e dois níveis de aptidão física aeróbia, não se observaram diferenças significantes no tempo limite após exercício intervalado de alta intensidade. A recuperação com uso de bicicleta apresentou maior remoção de lactato após a segunda série de esforço e a recuperação com exercícios do core exibiu maior contribuição na diminuição da frequência cardíaca durante os períodos de recuperação entre os dois blocos de esforços. / Introduction: In the prescription of high intensity interval training (HIIT) the recovery time between efforts is relevant aspect. However, the type of recovery has been shown to be a determinant of subsequent performance, and most studies have indicated that active recovery (RA) tends to be superior to passive (PR) recovery. Specifically regarding RA, it can be with the same muscle group exercised, with opposite body segment or with trunk region. At the However, the latter strategy has been little investigated. Objective: To investigate the effects of two types of RA on physiological responses and physical performance subsequent to high intensity interval training, considering two different levels of aerobic fitness. Materials and methods: The study was developed with 25 men, aged between 18 and 35 years, who were allocated in one of two groups: High Aptitude (AACR), Low Aptitude (BACR). Those involved had three days of activities. At the first visit, body mass, height, skinfolds, heart rate, blood lactate and resting blood pressure were measured, Of maximum power test (Pmax) on cycle ergometer and time-out test (TLim to 120% of Pmax), fifteen minutes after. The classification in AACR or BACR was from the Pmax obtained in the progressive test in cycle ergometer, and allocation according to the median. In the two and three views the subjects performed: i) standardized heating, ii) HIIT with two blocks with four supramaximal stimuli each (30 s at 60 rpm and with 120% load of Pmax, 30 s of passive recovery) and iii) TLim after Or HIIT. After heating, between the HIIT blocks and before the TLim, participants performed one of two modes of active recovery: active bike recovery and active recovery with core stabilization exercises. In the bike recovery, 30% of the Pmax was cycled for 3 min and in the core, three exercises were performed, each lasting 50 s. To analyze the data, we counted With descriptive statistics and data were analyzed with two-way analysis of variance (aerobic fitness level and recovery type) with repeated measurements. We assumed p <0.05 as a level of significance. Results: There was no statistically significant difference considering the level of fitness and type of recovery for TLim. For core recovery, BACR and AACR reached, respectively, 118 ± 25 s and 142 ± 62 s. In bike recovery, the values ​​were 110 ± 24 s and 134 ± 72 s. The bike recovery provided lower values ​​of blood lactate, but only before the second series of efforts (BACR: 3.62 ± 0.76mmol for core and 2.99 ± 0.90mmol for bike; AACR: 3.23 ± 0.52mmol for core and 2.83 ± 0.66mmol for bike, F = 6.38 , P = 0.01). A9 core recovery, in turn, decreases heart rate more pronouncedly before the first and second series of HIIT, as well as before TLim (F≥33.8, p <0.001). Conclusion: Considering two types of active recovery and two levels of aerobic physical fitness, no significant differences were observed in the time limit after high intensity interval exercise. Recovery with bicycle showed greater lactate removal after the second effort series and recovery with core exercises showed a greater contribution in decreasing heart rate during the recovery periods between the two blocks of effort.