Within this work, a set of depositions induced by focused electron beam was prepared. The depositions were prepared in the presence of water vapours from trimethyl(methylcyclopentadienyl)platinum(IV) precursor. The dependence of prepared materials purity on beam accelerating voltage and water vapour pressure was measured. The best platinum content was achieved at (27,2 ± 0,4) at. %, with beam accelerating voltage 5 kV, beam current 1600 pA and water vapour pressure 100 Pa. Due to subsequent long-term exposure to light, air humidity and air oxygen, the platinum content was increased to (39,2 ± 2,1) at. %.

The main goal of the thesis was a selection of appropriate thermal cycles for the considerated nuclear power stations with the Generation IV reactors. Characterization and specification of the parametres of the Brayton and the Rankine-Clausi thermodynamic cycle, their optimalization with regard to the parametres of the first cycle was made, than analysis of cycles´properties, mostly efficiency, output and process layout.

The aim of this work was to find a correlation between the electrical, optical and microstructural properties of thin SiO2 layers containing group IV nanostructures produced by ion beam synthesis. The investigations were focused on two main topics: The electrical properties of Ge- and Si-rich oxide layers were studied in order to check their suitability for non-volatile memory applications. Secondly, photo- and electroluminescence (PL and EL) results of Ge-, Si/C- and Sn-rich SiO2 layers were compared to electrical properties to get a better understanding of the luminescence mechanism.

<p>Cardiovascular diseases (CVD) and their risk factors remain the leading cause of morbidity and mortality in North America. Food-derived bioactive peptides (BAP) have been shown to play a role in regulating physiological pathways of CVD risk factors including hypertension, diabetes, and chronic inflammation. Common sources of BAP include dairy and plant proteins. In addition to being an alternative protein source, it is now accepted that edible insect proteins can also confer health benefits beyond nutrition. However, as with any novel protein source, allergenicity remains a major concern surrounding edible insect consumption. </p> <p>This dissertation aimed to: 1) Evaluate the bioactive potential of peptides from an edible cricket species and; 2) determine the effects of BAP production methods on immunoreactivity. First, peptide-rich extracts were generated from farmed food-grade crickets via enzymatic hydrolysis techniques with the commercial protease Alcalase™. To measure the <i>in vitro</i> bioavailability, cricket peptides were also subject to simulated gastrointestinal digestion (SGD). Peptides and their digests were tested for relevant bioactivities and active groups were further fractionated by chromatographic methods to identify the major peptides responsible for the bioactivity. When tested for <i>in vitro</i> antihypertensive and anti-glycemic properties, cricket peptides were found to inhibit the activities of angiotensin converting enzyme, dipeptidyl peptidase-4, α-amylase, and α-glucosidase. The anti-inflammatory potential was expounded using RAW-264.7 macrophages and human umbilical vein endothelial cells (HUVEC). Cricket peptides (after SGD) effectively lowered NF-κB, MCP-1, and IL-6 production in cells without affecting their viability. Proteomic analyses identified 18 sequences from the enriched cationic peptide fraction that showed the highest activity. Three novel peptides were identified via molecular docking, as potent ACE-inhibitors and binding was similar to that of the commercial drug captopril. Key binding characteristics included interaction with hydrophobic amino acids (Phe, Pro, Leu) near the C-terminal position and coordination with Zn (II) ions near the ACE active site.</p> <p>Immunological reactivity was measured by IgE-binding from shrimp-allergenic patient sera to antigens present within cricket peptides. Our studies demonstrate that immunoreactivity was impacted by enzymatic hydrolysis, depending on the conditions and heating source used. Tropomyosin (a major shrimp allergen) was extracted from both untreated crickets and protein hydrolysates, and verified as the major reactive protein. Tropomyosin reactivity decreased (under both partial and extensive hydrolysis) or retained (under conditions which prevented epitope cleavage). However, using microwave-assisted enzymatic hydrolysis was effective at decreasing tropomyosin reactivity in all immunoassays tested (IgG and IgE). Proteomic and immunoinformatic analyses revealed prominent allergen binding regions of cricket tropomyosin available for cleavage during enzymatic hydrolysis. Conserved antigen regions showed greater homology with other crustacean species, but not with other well studied allergenic insect proteins (i.e., cockroach). Lastly, LC-MS/MS and FT-Raman spectrometry suggests that reactivity was affected due to distinct epitope cleavage within the protein instead of decreased antigen extractability/solubility. </p> <p>The findings of this dissertation support that edible cricket proteins are a potential source of bioactive peptides for functional food or nutraceutical development. Additionally, using protein extraction methods such as microwave-assisted hydrolysis seems a promising tool for minimizing the immunoreactivity of the allergen present in this edible cricket species.</p>

Introduction: This paper does not aim to predict the future of clinical psychology in Germany. The future of psychology depends on the complex interaction between political, sociological, economic and health-care related factors as well as on the scientific progress in the discipline itself and in neighbour disciplines. However, it is fair to say that clinical psychology continues to gain even stronger influences in health care and will face a number of new challenges over the next years of its expansion. Our paper will present some of these potential fields of development and change based on a brief description of the status quo. The focus of the article will be specific developments in Germany, although there will be an overlap with general tendencies that describe the situation of clinical psychology in the new millenium in general. Furthermore, for research as well as practice, the specific relationship between clinical psychology and psyciatry will be highlighted.

The Convent of Dominicans on the Hare Island in Budapest. The topic of the master thesis is the architectural analysis of the Nunnery of Dominicans on the Hare Island in Budapest, which was established with Bela the IV. to his daughter Margaret - later Saint Margeret of Hungary. The Nunnery was originated around the middle of the 13th century (the most probably between 1246-1252). And is presented as an example of the archtitecture of Nunnery of the Orders of Dominicans in Europe. This master thesis presents the history of this Nunnery and also the origin of the guild of the nunnery. The guild was working all around Buda for the king of Hungary - Béla IV. Some of the Hungarian Art Historians think, that the guild originally came to Hungary after they finished their works on the Nunnery of the Cistercianer Order in Porta Coeli in Tišnov near Brno. The person of Saint Margaret of Hungary and her Cult in Hungary and Europe is also discussed in this master thesis.

Philosophiae Doctor - PhD / Immunosensors are affinity ligand-based biosensor solid-state devices in which the immunochemical reaction is coupled to a transducer. The specificity of the molecular recognition of antigens by antibodies to form a stable complex is the basis of the immunosensor on the electrode. The development of such a sensor requires a better design and preparation of an optimum interface between the biomolecules and the detector material. The immunosensors were developed based on Polyaniline derivative composite. Novel water soluble PdTe quantum dots (QD) was synthesized and characterized by different physical techniques such as UV-Visible (UV-VIS), Fluorescence Spectroscopy (PL), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and X-Ray Diffraction (XRD). The electroactivity of such synthesized quantum dots was studied by cyclic voltammetry in aqueous media. The synthesis of poly(2,5- dimethoxyaniline)-multi wall 'carbon nanotubes nanocomposite was carried out by electropolymerization in situ of 2,5-dimethoxyaniline - multi wall carbon nanotubes (PDMA-MWCNT) from aqueous dispersion containing acid-treated multi wall carbon nanotubes (MWCNT) and 2,5-dimethoxyaniline subsequently modifying a glassy carbon electrode in acid media. An undoped PDMA was also prepared for control. The composite for this work, consists of layer-by-layer method to form a multilayer film of QDs and PDMA-MWCNT. The method used was as follows; the drop coating of quantum dots followed by electrodeposition of poly(2,5- dimethoxyaniline )-carbon nanotubes onto surface of glassy carbon. The PDMA-CNT was characterized by UV-Visible (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The electrochemical characterisation of PDMA-CNT was carried out using cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). The composite (QDs-PDMA-MWCNT) was also characterized using above mentioned techniques. The electrochemical immunosensor for fumonisin a mycotoxin was prepared by dropcoating of mycotoxins antibody onto the composite modified glassy carbon electrode. The response profiles of fumonisins sensors system were obtained from electrochemical impedance spectroscopy (EIS) measurements. The fumonisin immunosensor was used for the detection of fumonisins in certified com reference materials. For comparison reasons, analysis of such mycotoxins was carried out by using conventional analytical method enzyme-linked immunosorbent assay (ELISA). The EIS response of FBI immunosensor (GCEIPT-PDMA-MWCNT/anti-Fms-BSA) gave a linear range of 7 to 49 ng L-I and the corresponding sensitivity and detection limits were 0.0162 ka L ng-I and 0.46 pg L-I, respectively. Hence the limit of detection of GCEIPT-PDMA-MWCNT immunosensor for fumonisins in com certified material was calculated to 0.014 and 0.011 ppm for FBI, and FB2 and FB3, respectively. These results are lower than those obtained by ELISA, a provisional maximum tolerable daily intake (PMTDI) for fumonisins (the sum of FBI, FB2, and FB3) established by the Joint FAO / WHO expert committee on food additives and contaminants of 2 ug kg" and the maximum level recommended by the U.S. Food and Drug Administration (FDA) for protection of human consumption (2-4 mg L-I).

I uppsatsen undersöks hovbildhuggaren Johan Tobias Sergels teckningar av samtida föräldraskap från 1700-talets andra hälft – en dramatiskt föränderlig period i Sveriges och Europas historia, präglad av upplysningsfilosofins omvälvande framväxt. Flertalet teckningar skildrar Sergels egen familj, och kung Gustav III i relationen till sonen och tronarvingen Gustav (IV) Adolf. Genom bildanalyser med stöd i en semiotisk begreppsapparat, samt analys av Sergels samtid, utforskas teckningsmotivens betydelser och hur de kan förstås i den historiska kontexten. Undersökningen visar hur två tydligt definierbara teman återkommer i Sergels teckningar (ömhet, intimitet och fysisk interaktion och pedagogik och utbildning), och hur motiven kan förstås som uttryck för upplysningsfilosofiska idéer om föräldraskap och familjelycka. Särskilda bildelement kan tolkas som tecken för strömningens progressiva moder- och faderskapsideal, som uttryckts av bl.a. upplysningsfilosofen Jean-Jacques Rousseau i Émile ou de l’education (1762), och återkommer i läroboken Barnabok (1780), tillägnad den då tvåårige Gustav (IV) Adolf. Undersökningen visar också hur Sergels skildringar av den egna familjen och det kungliga faderskapet, kan betraktas som konstnärliga tolkningar av då rådande familjeförhållanden. Ett urval brev till och från Sergel, samt tidigare forskning, styrker bilden av att Sergel, hans sambo Anna Rella och kung Gustav III praktiserade föräldraskap i linje med centrala upplysningsfilosofiska värden.

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