Com o objetivo de avaliar o desenvolvimento e qualidade de mudas de três espécies nativas do Cerrado, em função de lâminas de irrigação e tamanhos de recipiente, três experimentos foram conduzidos em casa de vegetação, em Piracicaba, São Paulo. As espécies utilizadas foram o jatobazeiro (Hymenaea courbaril L.), a pitangueira (Eugenia uniflora L.) e o baruzeiro (Dipteryx alata Vog.), nos Experimentos I, II e III, respectivamente. Os experimentos foram instalados no delineamento em blocos ao acaso, com três repetições, em parcelas subdivididas, no esquema 2 x 5. Nas parcelas, utilizaram-se dois tamanhos de recipientes, e nas subparcelas, cinco lâminas de irrigação (20%, 40%, 60%, 80% e 100% da evapotranspiração potencial - ETp, obtida por pesagem das mudas). Cada subparcela foi constituída por oito mudas, espaçadas em 22,5 cm entre si. Os recipientes, no Experimento I, foram vasos de 3,1 L e citrovasos de 4,0 L, enquanto nos Experimentos II e III, foram utilizados vasos (2,3 L) e sacos de polietileno (2,0 L). O substrato foi constituído de uma mistura de 50% substrato comercial e 50% areia fina (v/v). Para cada 100 L da mistura, foram adicionados 15 L de vermiculita. Utilizou-se o sistema de irrigação por gotejamento, com emissores autocompensantes de 2,0 e 4,0 L h-1, em diferentes combinações para atender as lâminas. As irrigações foram realizadas diariamente. Durante o período experimental, foram registrados em datalogger, a temperatura, a umidade relativa e a radiação solar global, e em tanque Classe A, a evaporação. Aos 80, 120, 160 e 200 dias após a semeadura (DAS), foram avaliados a altura, o diâmetro de caule e o número de folhas. Aos 120, 160 e 200 DAS, foram mensurados a área foliar, o comprimento do sistema radicular, as massas de matéria seca de raízes, caule, folhas e de muda, a relação das massas de matéria seca de raízes e parte aérea (MSR/MSPA) e a qualidade das mudas (Índice de Qualidade de Dickson - IQD). O coeficiente de cultivo (Kc) foi determinado em cinco fases de desenvolvimento. Lâminas de irrigação de 90,1% a 100% da ETp proporcionaram maior desenvolvimento vegetativo e IQD das mudas de H. courbaril L., até 200 dias de idade. Com relação aos recipientes, maior desenvolvimento de mudas de jatobazeiro, pitangueira e baruzeiro foi observado no citrovaso, no vaso (2,3 L) e no saco plástico, respectivamente. Mudas de pitangueira e de baruzeiro apresentaram maior desenvolvimento e IQD, na lâmina de irrigação de 100% (ausência de deficit hídrico). Maior ac úmulo de matéria seca pelo jatobazeiro e baruzeiro, foi observado de 120 a 160 DAS, e pela pitangueira, de 160 a 200 DAS. Mudas de pitangueira submetidas a deficit hídrico mais intenso, se mostraram resistentes à seca, em razão da maior relação MSR/MSPA. Nos primeiros 200 dias após semeadura, o Kc das espécies, obtido pela metodologia de Penman-Monteith apresentou os seguintes valores: 0,54 a 1,01 (H. courbaril L), 0,35 a 0,67 (E. uniflora L.) e 0,52 a 0,85 (D. alata Vog), sendo maior na fase de 160 a 200 DAS. / Aiming to evaluate the development and seedlings quality of three native plants species of the Savannah, in function of irrigation levels and container sizes, three experiments were carried in greenhouse, in Piracicaba, São Paulo. The species used were jatoba plant (Hymenaea courbaril L.), Surinam cherry (Eugenia uniflora L.) and \"baru\" plant (Dipteryx alata Vog.) in the Experiments I, II and III, respectively. The experiments were installed in randomized blocks design, with three replications, in split-plot, in the scheme 2 x 5. Two sizes of containers were used in the plots, and five irrigation levels (20%, 40%, 60%, 80% and 100% of the potential evapotranspiration - ETp, obtained by weighting of seedlings) were used in the subplots. Each subplots was constituted by eight seedlings, at the spacing 22.5 cm between themselves. The containers in the Experiment I, were pots 3.1 L and stiff plastic tube of 4.0 L, while in the Experiments II and III, pots (2.3 L) and container of polyethylene (2.0 L) were used. The substrate was constituted of 50% commercial substrate and 50% fine sand (v/v) mixture. Fifteen liters of vermiculite were added per each 100 L of that mixture. The drip irrigation system was used, with self compensating emitters at flow of 2.0 and 4.0 L h-1, in different combinations to satisfy the irrigation levels. The irrigations were accomplished daily. During the experimental period, the temperature, relative humidity and global solar radiation were recorded in data logger, and the evaporation was mensured in class A pan. The seedling height, the stem diameter and leaves number were appraised at the 80, 120, 160 and 200 days after sowing (DAS), while the leaf area, the root system length, the dry matter mass of roots, stem, leaves and seedling, the ratio between roots dry matter mass and aerial part (MSR/MSPA) and seedlings quality (Dickson Quality Index - IQD) were measured at 120, 160 and 200 DAS. The crop coefficient (Kc) was determined in five stages of the species development. Irrigation levels between 90.1% and 100% of ETp provided larger vegetative development and IQD of H courbaril L. seedlings, up to 200 days of age. Regarding the containers, larger development of the H. courbaril L., E. uniflora L. and D. alata Vog. seedlings was obtained in the stiff plastic tube, the pot (2.3 L) and the polyethylene container, respectively. E. uniflora L. and D. alata Vog. seedlings presented larger development and IQD, under the irrigation level 100% (absence of water deficit). Larger dry matter mass production of the H. courbaril L. and D. alata Vog. seedlings was observed between 120 and 160 DAS, and of the E. uniflora L., in the stage from 160 to 200 DAS. E. uniflora L. seedlings under to more intense water deficit, they presented resistance to the drought, in reason of the largest ratio MSR/MSPA. Seedlings of up to 200 days after sowing, the Kc presented the following values, by methodology of Penman-Monteith: 0.54-1.01 (H. courbaril L), 0.35-0.67 (E. uniflora L.) and 0.52-0.85 (D. alata Vog.), being larger in the stage from 160 to 200 DAS.

<p>Automatsko prepoznavanje govora je tehnologija koja računarima<br />omogućava pretvaranje izgovorenih reči u tekst. Ona se može<br />primeniti u mnogim savremenim sistemima koji uključuju komunikaciju<br />između čoveka i mašine. U ovoj disertaciji detaljno je opisana jedna<br />od dve glavne komponente sistema za prepoznavanje govora, a to je<br />jezički model, koji specificira rečnik sistema, kao i pravila prema<br />kojim se pojedinačne reči mogu povezati u rečenicu. Srpski jezik spada<br />u grupu visoko inflektivnih i morfološki bogatih jezika, što znači<br />da koristi veći broj različitih završetaka reči za izražavanje<br />željene gramatičke, sintaksičke ili semantičke funkcije date reči.<br />Ovakvo ponašanje često dovodi do velikog broja grešaka sistema za<br />prepoznavanje govora kod kojih zbog dobrog akustičkog poklapanja<br />prepoznavač pogodi osnovni oblik reči, ali pogreši njen završetak.<br />Taj završetak može da označava drugu morfološku kategoriju, na<br />primer, padež, rod ili broj. U radu je predstavljen novi alat za<br />modelovanje jezika, koji uz identitet reči u modelu može da koristi<br />dodatna leksička i morfološka obeležja reči, čime je testirana<br />hipoteza da te dodatne informacije mogu pomoći u prevazilaženju<br />značajnog broja grešaka prepoznavača koje su posledica<br />inflektivnosti srpskog jezika.</p> / <p>Automatic speech recognition is a technology that allows computers to<br />convert spoken words into text. It can be applied in various areas which<br />involve communication between humans and machines. This thesis primarily<br />deals with one of two main components of speech recognition systems - the<br />language model, that specifies the vocabulary of the system, as well as the<br />rules by which individual words can be linked into sentences. The Serbian<br />language belongs to a group of highly inflective and morphologically rich<br />languages, which means that it uses a number of different word endings to<br />express the desired grammatical, syntactic, or semantic function of the given<br />word. Such behavior often leads to a significant number of errors in speech<br />recognition systems where due to good acoustic matching the recognizer<br />correctly guesses the basic form of the word, but an error occurs in the word<br />ending. This word ending may indicate a different morphological category, for<br />example, word case, grammatical gender, or grammatical number. The<br />thesis presents a new language modeling tool which, along with the word<br />identity, can also model additional lexical and morphological features of the<br />word, thus testing the hypothesis that this additional information can help<br />overcome a significant number of recognition errors that result from the high<br />inflectivity of the Serbian language.</p>

Title: Screening of musculoskeletal system and level of physical activity in children of younger school age Objectives: The aim of the study is to asseess physical activity of school age in primary school in Dolní Břežany and try to find the most common risk factors for motor development of children in school age. Another aim of the study is to find statistical context between flat foot and age, gender, hypermobility, shortened hamstring muscles, lumbar hyperlordosis, level of core muscles and overweight and between winged scapula and age, gender, hypermobility, shortened m. trapezius and mm. pectorales and level of core muscles. Methods: The research was carried out by using non-standardized questionnaire to assess physical activity. Level of motor development was evaluated by group of physiotherapists. The research population was chosen purposefully by contractual research which consisted of 298 children who study on primary school in Dolní Břežany. For statistical analysis of all measured was used Microsoft Excel software. These data were evaluated by Chi - squared test and logistic regression. Results: It was found that the largest number of children does sports 2-4 hours a week. The most common risk factor of motor development of children is winged scapula and shortened hamstring muscles....

Im Zuge der amtlichen Fleischhygieneuntersuchungen auf Trichinellen im Wildschweinfleisch wurde regelmäßig ein weiterer Parasit, die Mesozerkarie von Alaria alata gefunden. Es wurden daraufhin Anfang der 2000er Jahre Prävalenzstundien an Wildschweinfleisch durchgeführt. Zu einer flächendeckenden Erhebung von Daten in Deutschland kam es jedoch nicht und so wurde 2007 vom BfR keine gezielte Untersuchung empfohlen. In folgenden Studien etablierten führende Wissenschaftler eine Methode zur Detektion des Duncker`schen Muskelegels (DME), die Alaria alata mesocercariae migration technique (AMT) (RIEHN et al. 2010), da sich die Trichinellenuntersuchung als fehlerhaft und unzureichend zum Zweck der Detektierung von DME herausstellte. Die AMT führte zu weitaus höheren Nachweisen in den untersuchten Proben, als Zufallsbefunde hätten vermuten lassen können. Trotz großer Bedeutung für die Detektierung des DME ist die AMT als etablierte und evaluierte Methode kein amtlich vorgeschriebenes Verfahren zur routinemäßigen Untersuchung von Wildschweinfleisch. Neben dem Interesse die bestehende Gefahr in verarbeiteten Lebensmitteln zu untersuchen und Verfahren zur Risikominimierung zu erarbeiten, war es dringend notwendig Daten über das Vorkommen und die Verbreitung sowie die Anwendung der AMT in praxi zu sammeln. Für den Landkreis MSE lagen keine Daten zum Vorkommen des DME in der Wildschweinpopulation vor. In der routinemäßigen Fleischuntersuchung wurde kaum DME gefunden, obwohl die geografischen Gegebenheiten ein Vorkommen begünstigen. Die AMT sollte als Methode der Wahl zur Detektierung von DME in praxi geprüft werden. In Anlehnung an vorangegangene Studien sollten Daten zur Befallsintensität und zur Befallshäufigkeit erhoben werden. Eingeschlossen werden sollten saisonale Unterschiede, Alters-, Geschlechts- und Gewichtsunterschiede. In einem Zeitraum von 09/2014 bis 09/2015 wurden 421 Wildschweine untersucht. Dabei wurden Gewebeproben von vier definierten Stellen am Tierkörper entnommen (Backe, Bauch, Zunge, Zwerchfell). Und mittels AMT untersucht. Der Landkreis wurde in seine 19 Ämter und in die Stadt Neubrandenburg aufgeteilt, die wiederum in drei Altkreise statistisch zusammengefasst wurden. Geografische Besonderheiten bei der Gewässerstruktur wurden beachtet. Es wurden insgesamt 1330 Proben untersucht und eine Prävalenz für MSE von 40,1 % ermittelt. Im Ämtervergleich ist das Amt Seenlandschaft Waren (aus dem Altkreis WRN) mit einer Prävalenz von 63,3 % hervorzuheben. Die Auswertung des Entnahmezeitraums zeigte Schwankungen der Prävalenzen mit signifikant hohen Nachweisen sowohl in den Wintermonaten (Dezember 56,7 %, Januar 46,1 %) als auch in den Sommermonaten (Juni 76,5 %, Juli 69,2 %). Im Altersklassenvergleich traten am meisten positive Tiere in der AK 1 (46,2 %) auf. Im Gewebevergleich waren die Backe (max. 82DME/ Probe) und die Zunge (max. 33DME/ Probe) am höchsten mit DME befallen (in allen untersuchten Proben: Backe 592 DME; Zunge 314 DME). Dabei wurden im Altkreis WRN 907 von insgesamt 1166 DME gefunden. Schlussfolgerung: Die Ergebnisse dieser Arbeit unterstützen die bisherigen Daten. Die Anwendung der AMT ist für den routinemäßigen Einsatz geeignet. Im Landkreis MSE lassen sich geografischen Abhängigkeiten zwischen gewässerreichen und gewässerärmeren Gebieten aufzeigen. Es konnten keine statistisch signifikanten Alters-, Gewichts- und Geschlechtsunterschiede festgestellt werden. Die vorliegenden Daten ermöglichen eine Erweiterung rechtlicher Bewertungen der amtlichen Fleischuntersuchung und tragen einen entscheidenden Beitrag zur Ausrichtung des regionalen Verbraucherschutzes bei.

Le travail présenté porte sur le développement d’une nouvelle technologie innovante, nommée PAT Friday®, visant à produire des protéines recombinantes au sein des sécrétions extracellulaires de plantes carnivores génétiquement modifiées. Deux objectifs ont été fixés : Réaliser la preuve de concept de la technologie sur le modèle expérimental Drosera rotundifolia, en transformant la plante avec des gènes marqueurs et humains afin de mettre en évidence la présence des protéines recombinantes dans la glu ; et développer, après évaluation, la technologie sur un modèle potentiellement industrialisable, Nepenthes alata. Les résultats ont indiqué la présence des deux protéines marqueurs GFP et GUS dans les tissus et dans la glu de Drosera rotundifolia transformées. Les plantes ont également été transformées génétiquement avec les gènes humains de l’interféron gamma et du facteur intrinsèque. Les protéines recombinantes humaines ont été mises en évidence au sein des tissus végétaux. Le potentiel industriel du modèle Nepenthes alata a ensuite été étudié : 10 à 15 kg de protéines totales par hectare et par an peuvent être produits, grâce notamment à des récoltes successives non destructrices, et la possibilité de contrôler l’activité des protéases digestives naturelles. L’élaboration d’un protocole de régénération de la plante a été entreprise par embryogénèse somatique et organogénèse indirecte, en vue de sa transformation génétique. La technologie PAT Friday®, avec des étapes simplifiées d’extraction et de purification des protéines d’intérêt produites dans le liquide digestif, offre de nouvelles perspectives dans le domaine des protéines thérapeutiques produites à partir de plantes / The present work focuses on the development of a new innovating technology, called PAT Friday®, aiming at producing recombinant proteins into the extra-foliar fluid of modified carnivorous plants. Two objectives were assigned to this work : 1- to realize a proof of concept of the technology on the experimental model Drosera rotundifolia, transformed with marker and human genes, to confirm the occurence of the recombinant proteins into glu ; and 2 - to evaluate and develop, the technology on the model Nepenthes alata, more adapted to industrial scaling-up. The results indicate the presence of two marker proteins GUS and GFP inside the tissues and into the glu of modified Drosera rotundifolia plants. The same plant species has also been transformed with human gamma interferon and intrinsic factor genes. The corresponding human recombinant proteins have been detected into the plant tissues. Potential industrial scaling-up has been studied with the species Nepenthes alata. The results show a potential productivity of 10 to 15 kg of total proteins per hectare per year, thanks to non-destructive repeated harvests, and possibility to efficiently control the natural proteinase activity. The elaboration of a regeneration protocol has been undertaken through indirect organogenesis and somatic embryogenesis, with a view to transform genetically this plant. PAT Friday® technology, with simplified extraction and purification methods of the proteins of interest targeted into the liquid secretions, opens new perspectives in the field of therapeutical proteins produced in plants

<p>Predmet istraživanja ove disertacije predstavlja unapređenje, modelovanje i optimizacija procesa elektroerozivne obrade (EDM) savremenih inženjerskih materijala. Prvo su predstavljene dve inovativne metode: EDM u dielektrikumu sa pome&scaron;anim prahom i EDM sa pomoćnom elektrodom, a zatim i njihova kombinacija. Za generisanje matematičkih modela primenjene su metodologija odzivne povr&scaron;ine i alati ve&scaron;tačke inteligencije. U nastavku su postavljeni optimizacioni procesi određivanja ulaznih parametara sa jednom i vi&scaron;e funkcija cilja koji su re&scaron;eni primenom klasičnih metoda optimizacije. U zavr&scaron;nom osvrtu sprovedena je verifikacija dobijenih modela i optimalnih ulaznih parametara elektroerozivne obrade.</p> / <p>The subject of the research of this dissertation is the improvement, modeling and optimization of the electrical discharge machining (EDM) of advanced engineering materials. First, two innovation methods are presented: EDM in powder mixed dielectric fluid and EDM with assisted electrode and that their combination. The method of response surface and artificial intelligence tools were applied to generate mathematical models. The optimization problems of determining the input parameters with single and multiple target functions are solved by the application of classical optimization methods. Finally, verification of the obtained models and optimal input parameters of electrical discharge machining was carried out.</p>

Made available in DSpace on 2014-07-29T14:52:09Z (GMT). No. of bitstreams: 1 Tese thannya nascimento.pdf: 3166640 bytes, checksum: 0a9faeb659c9594423ee5e7f8b495662 (MD5) Previous issue date: 2009-01-22 / The goal of this study was to evaluate the genetic structure and the spatial pattern of intra and interpopulational gene flow of Dipteryx alata Vogel, based on nuclear and chloroplastidial microsatellite markers. Primers were developed based on sequencing of random fragments from a shotgun genomic library for detection of microsatellite regions. 12 microsatellites regions were obtained from 688 sequences, which allowed the construction of pairs of primers. These regions are composed of motifs with two to six nucleotides, ranging from 136 to 380 base pairs. This shows that the random sequencing strategy from shotgun libraries is interesting because it allows the achievement of primers for repetitive regions with different motifs. Two of these loci (Da_E06 e Da_E12) were polymorphic with three alleles each. He estimation for these loci showed satisfactory values (0.2946 and 0.2879, respectively), considering the number of alleles. Also, we used a transferred primer from the species Phaseolus vulgaris (BM164) for D. alata. Moreover, other two chloroplastidials microsatellite primers were used for molecular analyses of georeferenced subpopulations, totalizing 775 plants distributed over the natural occurrence area of Cerrado. 210 of these plants were collected and georeferenced one by one along the margins of the Araguaia River in the states of Mato-Grosso (RAMT) and Goiás (RAGO) for spatial distribution of genetic variability in local scale analysis. The relationship between estimations of genetic diversity parameters with patterns of potential distribution of species was evaluated. This was used to test the hypothesis that the genetic variability of D. alata populations is distributed according to the central-periphery model. D. alata subpopulations showed considerable high levels of genetic variability that was significantly structured among subpopulations and well structured in space, both for nuclear and chloroplastidial data. The estimation of the apparent cross-fertilization rate (ta = 1.0575) indicates that the species is allogamous. Estimations of migration rates by pollen and by seeds were lower than one, indicating that seed dispersal contributes more effectively for total gene flow. Estimates of the genetic diversity parameters from the Araguaia River population showed similar values between both margins. The estimation of the apparent cross-fertilization rate (0.9434) indicated that the Araguaia River is not a physical barrier to effective gene flow. The effective size of the neighborhood, i.e., the mean number of individuals in an area where panmixia occurs was 85.64 and 22.99 for nuclear and chloroplastidial data, respectively, indicating that seed dispersal is more restricted. The correlogram generated with chloroplastidial data presented a cline pattern of variance more evident than with nuclear data, suggesting that the presence of spatial genetic structure is being more influenced by seed dispersal. We observed that the genetic parameters do not follow a classical central-periphery model, because peripheral population (according to geographical distribution of sampling locations) tended to demonstrate higher values for these estimations, mainly the South and Western subpopulations. The relationship found between the fixation index (f) with human impact indicated that the subpopulations evaluated can be affected by fragmentation process and land use, probably caused by the recent human colonization in Cerrado biome. / O objetivo geral deste estudo foi avaliar a estrutura genética e o padrão espacial do fluxo gênico intra e interpopulacional de Dipteryx alata Vogel, com base em marcadores microssatélites nucleares e cloroplastidiais. Foi utilizada uma estratégia de desenvolvimento de iniciadores que se baseia no seqüenciamento aleatório de fragmentos provenientes de uma biblioteca genômica shotgun para a detecção de regiões microssatélites, em barueiro. Das 668 sequências obtidas, foram encontradas 12 regiões microssatélites que possibilitaram a construção dos pares de iniciadores. Estas regiões são compostas por motivos com dois a seis nucleotídeos, que variam entre 136 a 380 pb. Isto mostra que a estratégia de sequenciamento aleatório a partir de bibliotecas shotgun é interessante por possibilitar a obtenção de iniciadores para regiões repetitivas com diferentes motivos. Dois destes locos (Da_E06 e Da_E12) foram polimórficos, apresentando três alelos cada. As estimativas de He para estes locos apresentaram valores satisfatórios, considerando o número de alelos, e foram iguais a 0,2946 e 0,2879, respectivamente. Isto indica que os iniciadores desenvolvidos e padronizados com sucesso neste trabalho podem ser utilizados para estudos genético-populacionais. Juntamente com estes dois iniciadores desenvolvidos, foi utilizado outro transferido da espécie Phaseolus vulgaris (BM164) para D. alata, além de outros dois iniciadores microssatélites cloroplastidiais para análises moleculares de 23 subpopulações georeferenciadas, totalizando 775 plantas, distribuídas ao longo da área de ocorrência natural do Cerrado. Destas plantas, 210 foram coletadas e georreferenciadas uma a uma, ao longo das margens do alto rio Araguaia nos estados de Mato-Grosso (RAMT) e Goiás (RAGO), para análises de distribuição espacial da variabilidade genética em escala local. Para testar a hipótese de que a variabilidade genética das subpopulações de D. alata se distribui conforme o modelo central-periférico, foram avaliadas as relações entre as estimativas de parâmetro genético de diversidade com os padrões de distribuição potencial da espécie. Foi observado que as subpopulações de D. alata apresentam consideráveis níveis de variabilidade genética que se encontra significativamente estruturada entre as subpopulações, tanto para os dados nucleares quanto para os cloroplastidiais, apresentando-se também estruturada no espaço. A estimativa da taxa de fecundação cruzada aparente (ta = 1,0575) indica que a espécie seja alógama. As estimativas das razões de migração via pólen e via semente foram menores do que 1, o que indica que a dispersão de sementes contribui mais efetivamente para o fluxo gênico total em escala regional. As estimativas dos parâmetros genéticos de diversidade referentes à população contígua ao Rio Araguaia mostraram valores semelhantes entre as margens do rio. A estimativa da taxa de fecundação cruzada aparente (0,9434) confirma a reprodução por alogamia no barueiro. O baixo nível de diferenciação entre as subpopulações das duas margens do rio indica que o rio Araguaia não confere uma barreira física efetiva ao fluxo gênico. O tamanho efetivo de vizinhança, ou seja, o número médio de indivíduos numa área onde ocorre panmixia, para os dados nucleares e cloroplastidiais foi igual a 85,64 e 22,99, respectivamente, indicando que a dispersão de sementes é mais restrita em escala local. O correlograma gerado a partir dos dados cloroplastidiais apresenta um padrão clinal de variação mais evidente do que o proveniente dos dados nucleares, o que sugere que a presença da estrutura genética espacial deve estar sendo mais influenciada pelo padrão de dispersão da semente. Foi observado que parâmetros genéticos estimados para D. alata não se enquadram no clássico modelo central-periférico, pois as populações tidas como periféricas (de acordo com a distribuição geográfica dos pontos de coleta) tendem a exibir maiores valores para estas estimativas, com destaque para as as subpopulações das bordas Sul e Oeste do bioma. A relação encontrada entre os valores estimados para o índice de fixação intrapopulacional (f) com o impacto humano indica que as subpopulações estudadas podem estar sofrendo as conseqüências dos processos de fragmentação e uso da terra na variabilidade genética, causado provavelmente pelo recente processo de ocupação do Cerrado.

No description available.