Introdução: O consumidor tem o direito a informações sobre a composição nutricional dos alimentos industrializados, possibilitando escolhas saudáveis. Para isto, a Agência Nacional de Vigilância Sanitária torna obrigatória a rotulagem nutricional dos alimentos comercializados. Objetivo: Avaliar a conformidade dos dados nutricionais presentes em rótulos de alguns alimentos embalados, comumente consumidos pelo público infantil, com o seu valor experimental, obtido através de análises por métodos oficiais e, desta forma, inferir a qualidade da informação disponível ao consumidor. Métodos: Foi feita a comparação dos dados declarados em rótulos de alimentos com os obtidos no laboratório. Foram selecionados alguns alimentos embalados consumidos pelo publico infantil, como salgadinhos de milho e de trigo de sabores e formas variadas, alguns tipos de batata frita, amendoim, biscoitos recheados, wafers, chocolates recheados ao leite e bombons de chocolate ao leite recheados. Foram avaliados os teores de proteínas, gorduras totais, gorduras saturadas, fibra alimentar e sódio, utilizando os métodos descritos nas "Normas Analíticas do Instituto Adolfo Lutz". O valor de carboidratos foi calculado pela diferença entre 100 e a soma da umidade, cinzas, protídios, gorduras totais e fibra alimentar, conforme a Resolução no 360/2003 da ANVISA. O valor calórico foi calculado utilizando-se os fatores de Atwater. Os dados foram tratados estatisticamente pela distribuição de freqüência percentual e foi calculado um Intervalo de Condenação a partir destes resultados. Resultados: O Intervalo de Condenação para o teor de proteína declarado na rotulagem nutricional variou de 0 a 50% sendo o maior índice para batata frita. Para o teor de carboidratos variou de 0 a 40%, sendo maiores para o amendoim e o salgadinho de milho. Quanto às gorduras saturadas, observou-se um Intervalo de Condenação entre 12 a 41%, sendo maior para a batata frita, amendoim e salgadinho de milho. Para o teor de fibra alimentar, variou de 8 a 69%. O Intervalo de Condenação para o teor de gorduras totais variou de 0 a 85% sendo que o maior índice observado foi para o salgadinho de milho. Quanto ao teor de sódio variou entre 4 e 72%. Para os produtos doces o Intervalo de Condenação do teor de gorduras totais variou entre 0 e 75%, sendo maior para o bombom recheado. O teor de gorduras saturadas a variação foi entre 0 e 52% sendo maior para o biscoito recheado, enquanto que, para o teor de fibra alimentar, foi entre 0 e 36% sendo a maior taxa para o biscoito recheado. O Intervalo de Condenação do teor de proteína variou entre 10 e 40%, sendo maior para o bombom recheado. Conclusão: Dentre os produtos analisados a batata frita, o salgadinho de milho e o amendoim tiveram a maior contribuição de condenações para parâmetros de teor de proteínas, carboidratos, fibra alimentar e ácidos graxos saturados, destacando-se como índices mais altos os teores de ácidos graxos saturados e de fibra alimentar. Quanto aos produtos doces o bombom recheado, o biscoito recheado e o wafer foram os que apresentaram maior Intervalo de Condenação para gordura total, gordura saturada e fibra alimentar. Os resultados permitem inferir que as informações na rotulagem nutricional nos produtos destinados ao público infantil e adolescentes não estão adequadas / The consumers have the right to informations about both the characteristics and the nutritional composition of the foods they buy, allowing them for healthy choices. Therefore, the Sanitary Inspection National Agency (ANVISA) makes nutritional labeling of the commercialized foods obligatory. Must be informed, among the nutritional informations, the following nutrients rates: carbohydrates, proteins, total fats, saturated fats, trans fats, sodium, alimentary fibers, and caloric value. Objective: The purpose of this work was to evaluate the conformity of nutritional data present in the labels of some packed foods, normally consumed by children, its experimental value being obtained by means of analyses by official methods, leading to an evaluation of the quality of the information available to the consumer. Methods: The data declared in foods labels were compared with those obtained in the laboratory. Some packed foods consumed by children were choose, like corn and wheat products of different flavors and forms, some types of potato chips, peanuts, filled biscuits, wafer biscuts, and filled milk chocolate, both in bars and in bonbons. The rates of proteins, total fats, saturated fats, alimentary fibers and sodium were evaluated utilizing the methods described in the "Normas Analíticas do Instituto Adolfo Lutz". The value for carbohydrates was calculated by the difference between 100 and total amount of humidity, ash, proteins, total fats and alimentary fibers, according to the Resolution 360 of ANVISA. The caloric value was calculated utilizing the Atwater factors. The data were treated statistically by the distribution of percent frequency. Results: The non-compliance of the protein rates declared on the nutritional labeling oscillated between 0 to 50%, with the highest index in the potato chips, that of carbohydrates varied between 0 and 40%, with the highest indexes found in peanuts and wheat snacks, that of saturated fat, 12 to 41%, the highest indexes being found in potato chips, peanuts and corn snacks, whilst fiber presented non-compliance going from 8 to 69%, whereby all products did not comply, total fats, 0 to 85%, the highest index found in the corn snacks. About the sodium, the disapproval interval oscillated between 12 and 72%. With regard to the sweet products, the condemnation interval for total fats oscillated from 0 to 75%, the highest percent rate being found in the chocolate bonbons. Saturated fats presented a disapproval interval between 0 to 52%, the widest one found in the filled biscuits. As for fibers, the disapproval interval went from 0 to 36%, the highest percent rate being found in the chocolate bonbons. The protein disapproval interval oscillated from 10 to 40%, the highest being in the filled milk chocolate bars and in the bonbons. Conclusion: Among the analyzed products, potato chips, corn snacks, and peanuts presented the highest disapproval rates by the parameters of proteins, carbohydrates, fiber, and saturated fat, where the rates of saturated fat acids and fiber stood out. Among the sweet products, chocolate bonbons, filled biscuits and wafer biscuits were the most condemned according to the parameters of total fats, saturated fats and fiber, leading to a preoccupation about the daily ingestion of these products by children and adolescents

The vapor phase photochemistry of the three isomeric cyanopyridines and the three methylpyridines was studied by irradiating their vapors at 254 nm. It was found that direct irradiation of any one cyanopyridine isomer resulted in the formation of the other two isomers of cyanopyridine or methylpyridines respectively. The reactivity of each isomer was found to be different. This was suggested to be based on the stability of their azaprefulvene intermediates formed during interconversion. The phototransposition of these molecules was suggested to result from 2,6-bonding, nitrogen migration around the five sides of cyclopentenyl ring followed by rearomatization. This mechanism was found to be consistent with the results of deuterium labeling studies of cyanopyridines These result suggest that cyanopyridines undergo phototransposition via the intermediacy of azaprefulvenes instead of Dewar-pyridine and azaprismane. Thus, photochemical studies showed that the six trideuteriopyridine isomers constitute two separate photochemical triads. Each triad consists of three isomers that are photointerconverting upon irradiation at 254 nm in the vapor phase. Similary, it was found that the three isomeric tetradeuteriopyridine isomers also constitute a photochemical triad and are interconverting upon irradiation at 254 nm in the vapor phase. These phototranspositions are best explained by the cyclization, nitrogen migration, and rearomatization mechanism. These results are in contrast to the long-held belief that pyridine is photostable in the vapor phase. Instead, unlabeled pyridine undergoes a hidden phototransposition leading back to itself.

Introdução. A epidemia global de obesidade e suas consequências ocorrem simultaneamente a uma maior consciência quanto às escolhas que promovem a saúde, padrões estéticos cada vez mais magros e oferta crescente de alimentos industrializados de alta densidade calórica, saborosos e práticos. Este cenário estimula a indústria de alimentos a utilizar apelos de saúde como estratégia de concorrência. Objetivo. Analisar a utilização de apelos de saúde e outras estratégias de incentivo ao consumo presentes na rotulagem de alimentos industrializados, sua coerência com os teores de calorias, gorduras, açúcares e sal presentes nesses alimentos e sua convergência com a legislação pertinente. Metodologia. A definição de teores elevados de calorias, gorduras, açúcares e sódio e dos quesitos que nortearam a análise foi feita a partir da legislação pertinente e da literatura. Um formulário foi criado e a análise baseou-se nas informações declaradas na rotulagem pelo fabricante. A categoria estudada foi Biscoitos e Salgadinhos. Os produtos foram escolhidos pela análise do gasto das famílias paulistanas com alimentação a partir da base de micro dados da Pesquisa de Orçamento Familiar (POF), da Fundação Instituto de Pesquisas Econômicas (FIPE). Foram identificadas 64 marcas e adquiridos 209 produtos em supermercados da cidade de São Paulo. Foram avaliados densidade calórica, teores de açúcar, gorduras saturada e trans, e sódio. Quatro dos quesitos tratam de diversas formas de estímulo ao consumo (apelos de saúde, selos de certificação, brindes e prêmios e apresentação do produto); o último trata da obrigatoriedade de informar os teores de gorduras, açúcar e sódio em destaque. Produtos com um ou mais teores elevados não deveriam apresentar nenhuma ação relativa aos quatro primeiros quesitos, e todos deveriam atender ao último, por ser exigência legal. Resultados. A totalidade dos produtos apresentou densidade calórica elevada e para 99% a quantidade de Kcal/100g foi maior que o dobro do limite; 48% não informaram os teores de açúcar separadamente; 22% declararam quantidades elevadas de açúcar; 64% apresentaram teores elevados de gordura saturada. Mais da metade dos produtos continha gordura vegetal, mas declarou 0% de gordura trans na porção; 35% eram alimentos livres de gordura vegetal; 53% apresentaram teores de sódio elevados sendo 17% entre 50% e 100% acima e 20%, mais que o dobro do limite. Noventa e quatro por cento dos produtos apresentaram teores concomitantemente elevados; 27%, calorias, gordura saturada e sódio; 20%, calorias e sódio e 17%, calorias e gordura saturada. Setenta e um por cento dos produtos com um ou mais dos teores elevados utilizaram apelos de saúde na sua rotulagem; 4%, selos de certificação de sociedades médicas; 6% bonificações, prêmios ou brindes e 16%, embalagens especiais; 66% dos produtos não apresentaram teores de gorduras, açúcar e sódio em destaque apesar de esta ser exigência legal. Conclusão. Os produtos estudados apresentaram um ou mais dos teores avaliados acima dos parâmetros estabelecidos; apelos de saúde e outras estratégias de marketing nutricional foram identificados em produtos que não deveriam ser associados a uma alimentação saudável ou ter seu consumo incentivado. / Introduction. The global epidemic of obesity and its consequences occur simultaneously to a greater awareness of the choices that promote health, aesthetic standards increasingly thin and an increasing availability of foods of high caloric density, tasty and practical. This scenario encourages the food industry to use health claims as competitive strategy. Objective. To analyze the use of health claims and other strategies to boost consumption present in food labeling, the consistency with the levels of calories, fat, sugar and salt in these products and its convergence with relevant legislation. Methodology. The definition of high levels of calories, fats, sugars and sodium and the questions that guided the analysis were based was based on the relevant legislation and the literature. A form was created and the analysis was based on the information declared on the label by the manufacturer. The category studied was Cookies and Snacks. Products were chosen by the analysis of the household spending from Sao Paulo city with food from the Household Budget Survey (POF) database, run by Economic Research Institute Foundation (FIPE). Sixty four brands were identified and 209 products were purchased in supermarkets in the city of São Paulo. Energy density, sugar content, saturated and trans fats, and sodium were evaluated. Four of the questions dealt with various forms of promotion (health claims, certification, gifts and prizes and product presentation) and one with the obligation to highlight the levels of fat, sugar and sodium. Products with high levels of one or more of these contents should not present any elements relating to the first four questions, and all of them should serve the latter, because it is a legal requirement. Results. All the products showed high caloric density and for 99% of them the amount of Kcal/100g was higher than twice the threshold; 48% did not report sugar levels separately; 22% informed high amounts of sugar; 64% had elevated levels of saturated fat. More than half of the products contained vegetable fat, but stated 0% trans-fat per portion; 35% were vegetable fat free products; 53% of the sample showed elevated levels of sodium; 17% informed sodium levels between 50% and 100% above while 20% report more than double the limit . Ninety-four percent of the products showed high levels simultaneously; 27%, calories, sodium and saturated fat; 20%, calories and sodium and 17%, saturated fat and calories. Seventy-one percent of the products with one or more elevated levels used health claims in its labeling; 4% presented medical societies seal certification, 16% used special packaging and 6% offered bonuses, prizes or giveaways; 66% of the products did not show the levels of fat, sugar and sodium highlighted despite it being a legal requirement. Conclusion. The products included in this study had one or more of the assessed levels above established parameters; health claims and other marketing strategies were identified in nutritional products that should not be associated with healthy eating or have their consumption encouraged.

L’Imagerie par Résonance Magnétique (IRM) est une technique prometteuse largement répandue dans les milieux hospitaliers. Elle est non invasive, présente une bonne résolution spatiale et permet de visualiser en profondeur dans un organisme vivant. Elle possède cependant quelques défauts, dont sa faible sensibilité. Pour palier ce problème, il est possible d'utiliser des espèces hyperpolarisables telles que le xénon. Cependant, n’étant spécifique d’aucun récepteur biologique, le xénon nécessite d’être vectorisé. Pour ce faire, des auteurs ont proposé son encapsulation dans une cage moléculaire capable de reconnaître la cible biologique à imager. Les meilleurs candidats à ce jour sont les cryptophanes.Nous nous sommes fixés comme objectif dans cette thèse de concevoir et de synthétiser de nouvelles cages plus adaptées pour les applications en IRM 129Xe ainsi que des biosondes pertinentes pour se rapprocher d’applications in vivo. Dans une première partie de ma thèse, nous nous sommes intéressés au développement de nouvelles cages afin d’étudier et d’affiner les propriétés d’encapsulation du xénon au sein des cryptophanes. Dans les parties suivantes, nous nous sommes concentrés sur la conception de biosondes par fonctionnalisation de cryptophanes déjà décrits pour diverses applications d’intérêt biologique. D’une part, nous avons évalué la possibilité de détecter des métaux de manière plus spécifique et plus sensible grâce à l’IRM xénon hyperpolarisé. D’autre part, nous avons travaillé sur la conception de biosondes bimodales, afin de coupler des techniques complémentaires d’imagerie médicale. / Today, Magnetic Resonance Imaging (MRI) is a powerful clinically used imaging method which provides three-dimensional images with excellent resolution. However, conventional molecular MRI techniques that rely on the observation of water protons still suffer from reduced sensitivity and often lack selectivity. The use of hyperpolarized xenon can improve both the selectivity and sensitivity of the MRI method. As xenon has no specificity for any biological receptor, it needs to be vectorized. For this purpose, authors have proposed to encapsulate xenon inside molecular cages functionalized to recognize specific biological targets. The best candidates so far as biosensors are cryptophanes.The aim of this work is to design and synthesize new cryptophanes that are better suited for 129Xe MRI applications and relevant biosensors for future in vivo applications. In a first part, new cages were developed in order to study the encapsulation properties of xenon inside different cryptophanes. Then, biosensors were synthesized by functionnalization of known water-soluble cryptophanes for different applications of biological interest. We have therefore assessed the possibility of detecting metal ions specifically in a very sensitive way thanks to 129Xe MRI. New bimodal sensors were also designed and tested.

Objectifs : Les thérapies régénératives cardiaques ont connu un essor considérable au cours des 10 dernières années. Malgré des effets positifs démontrés chez l’animal, les bénéfices cliniques obtenus chez l’homme sont encore relativement modestes. L’objectif de ce travail a été de mieux comprendre les facteurs liés à l’implantation des cellules souches grâce aux techniques de détection cellulaire en imagerie par résonance magnétique (IRM), afin d’optimiser la thérapie cellulaire cardiaque.Matériel et méthodes : Un protocole de détection cellulaire en IRM cardiaque in vivo ainsi qu’une méthode de détection des microvaisseaux en IRM cardiaque ex vivo haute résolution avec des séquences Susceptibility Weighted Imaging (SWI) ont été développés, puis mis en application pour l’étude de la vectorisation de progéniteurs des cellules endothéliales marqués magnétiquement par des nanoparticules d’oxyde de Fer et injectés par voie intraveineuse, ainsi que pour l’évaluation de l’intégration et de l’efficacité de cellules souches mésenchymateuses administrées via des patchs de fibrine cellularisés chez des rats adultes indemnes de toute pathologie (un groupe contrôle vs un groupe infarctus via ligature définitive de l’artère interventriculaire antérieure).Résultats : Après injection intraveineuse et malgré la vectorisation magnétique (n=16 rats), l’imagerie de détection cellulaire a montré qu’aucune cellule n’était implantée dans le myocarde et que les paramètres fonctionnels cardiaques n’étaient pas améliorés. Avec les patchs cellularisés (n=37 rats), la fraction d’éjection ventriculaire gauche (FEVG) était améliorée dans les groupes de patchs cellularisés par rapport aux groupes contrôles. La densité microvasculaire était augmentée dans la zone infarcie et peri-infarcie dans les groupes cellularisés par rapport aux groupes contrôles, à la fois en immunohistochimie et en IRM sur les séquences SWI. L’IRM a montré l’absence de migration des cellules dans le myocarde à partir du patch, confirmé en immunohistochimie. La persistance de cellules dans la zone d’implantation du patch à la surface épicardique à J21 post greffe et l’étude en cytométrie en flux des cytokines et facteurs de croissance produits par les cellules souches plaident pour une efficacité de la thérapie cellulaire en rapport avec la sécrétion de facteurs paracrines par les cellules souches.Conclusion : L’imagerie de susceptibilité magnétique permet d’une part d’étudier les vaisseaux myocardiques sur des séquences pondérées en SWI ex vivo et d’autre part d’évaluer l’implantation des cellules souches sur des séquences en écho de gradient T2* in vivo. Ces techniques ont permis de mieux caractériser le mode d’action des patchs cardiaques en tant que réservoir de facteurs paracrines pour le traitement de l’insuffisance cardiaque dans un modèle murin. Ces résultats confirment l’intérêt fort à développer et optimiser l’utilisation de biomatériaux intelligents délivrant spécifiquement des molécules d’intérêt comme les cytokines ou les facteurs de croissance et permettant ainsi de contourner les contraintes immunogènes et tératogènes liés aux cellules souches. / Objectives: Cardiac regenerative therapies have grown considerably over the past 10 years. Despite positive effects demonstrated in animals, the clinical benefits obtained in humans are still relatively modest. The objective of this work was to better understand the factors associated with implantation of stem cells through the cell detection techniques in magnetic resonance imaging (MRI) and to improve cardiac stem cell therapy in a murine model of myocardial infarction.Materials and methods: A protocol for cell detection with gradient echo T2* sequences in cardiac MRI in vivo and a method for detection of microvessels in cardiac MRI ex vivo with high resolution Susceptibility Weighted Imaging sequences (SWI) were developed and implemented for the study of vectorization of intravenously injected endothelial progenitors cells (EPC) and the integration and evaluation of the impact of mesenchymal stem cells (MSC) administered via cellularized fibrin patches. A permanent ligation of the left anterior coronary artery was performed in adult rats. The stem cells were magnetically labeled with iron oxide nanoparticles by endocytosis.Results: Cell detection imaging showed no cell implantation in the myocardium and no improvement in cardiac functional parameters after intravenous injection of EPC, despite the aid of magnetic vectorization (n = 16 rats). With a local administration of MSC via cardiac patches (n = 37 rats), the left ventricular ejection fraction (LVEF) was improved in cellularized patches groups compared to controls. Microvascular density was increased in the infarcted and peri – infarcted areas in cellularized patches groups compared to controls in immunohistochemistry and in MRI on SWI sequences. The MRI showed no migration of cells into the myocardium from the patch, as confirmed by immunohistochemistry and Perls staining. The persistence of MSCs on the epicardial surface at D21 after implantation and flow cytometry profiling of cytokines and growth factors produced by MSC argue for cell therapy effectiveness related to the secretion of paracrine factors by stem cells.Conclusion: Susceptibility imaging allows: (1) to study myocardial vessels on SWI sequences ex vivo and (2) to assess the implementation of stem cells on gradient echo sequences T2 * in vivo. These techniques have shown that cardiac patches act as a reservoir of soluble mediators which paracrinally target the angiogenesis in the treatment of heart failure in a murine model. This is in favor of a move towards “cell free” biomaterials containing only molecules of interest such as cytokines or growth factors to circumvent immunogenic and teratogenic constraints related to the use of stem cells.

Green marketi ng is aimed at directing a company‘s efforts to undertake the processes of designing a product, its promotion, pricing, and distribution in a way that can help to protect the environment. The current study is aimed at investigating the influence of green marketing practices including eco-labeling, green branding and green advertising on consumer buying behavior in Pakistan which is a developing country. The study has also analyzed the mediating effect of environmental knowledge in the relationship between green marketing practices and consumer buying behavior. The research useda quantitative approach and a structured survey questionnaire to collect data from 300 respondents. The study results revealed that green advertising and green branding has a significant positive relationship with consumer buying behavior while eco-labeling is not a significant predictor for this purpose. Moreover, environmental knowledge partially mediates the relationship between green marketing and consumer buying behavior. In this way, the current study has contributed to literature by analyzing the green marketing practices with and without the presence of environmental knowledge in Pakistan. The results of the research are helpful for marketers to emphasize more on green advertising being the strong predictor of consumer buying behavior. It also urges them to make their environmental friendly activities closer to reality in order to gain greater acceptability from consumers. However, the study has not taken into account alldimensions of green marketing and the sample responses belong to the population in only urban areas of Pakistan.

Food allergies within the United States and Europe have become exceedingly prevalent in children, adolescents, and adults. In the United States alone, over 12 million people have been diagnosed with at least one food allergy. If the allergen is accidentally consumed, the food allergy sufferer may suffer a life-threatening condition known as anaphylaxis. More than 200 U.S. food allergic consumers die annually as a result of anaphylaxis due to ingestion of food allergens. To safely manage food allergies, food allergy sufferers and stakeholders (such as caregivers) require clear and complete ingredient information to avoid certain allergens in packaged food products. Despite U.S. legislative mandates designed to assist the food allergic consumers and stakeholders to make safe food selection choices, food allergy labeling on consumer packaged food products remains unclear. To properly manage food allergy, it is imperative that food allergy stakeholders have access to clear and unambiguous food allergen ingredients located on the food product labels. The purpose of this research was to capture the impact of current United States food allergen labeling guidelines on U.S. food allergy stakeholders. With the elaboration likelihood framework, this research aimed to better understand how the congruence of allergen ingredient information on the Product Label Claim on the front of the package and Nutrition Facts Panel information on the back of the package affected food allergic consumers and stakeholders in evaluation of product packaging allergen information. A total of 223 food allergy stakeholders were administered four surveys depicting food allergen labeling variations found on U.S. food product labels. Seventeen hypotheses were formulated and analyzed with ANCOVA and t tests to measure the impact of congruence and elaboration of the food allergen messages on the food product labels, with a specific focus on purchase intention. Findings indicated that food allergy stakeholders had difficulties using and understanding existing food allergen labels under current U.S. food allergen labeling guidelines. The results also showed confusion among U.S. food allergic stakeholders with safe food selection choices when the label claims did not align with the actual allergen ingredient information. Research, public policy, and managerial implications were thoroughly discussed.

Este trabalho tem por finalidade avaliar os ganhos em eficiência energética através da classificação de níveis, atribuídos a sistemas de iluminação das instalações, com a aplicação da proposta de regulamentação para etiquetagem voluntária do nível de eficiência energética de edificações comerciais, de serviços e públicos, em fase de homologação. Foi realizada uma contextualização da importância da referida proposta de regulamentação, com uma abordagem dos principais sistemas de avaliação de desempenho ambiental de edifícios em âmbito internacional e no Brasil, considerando as principais iniciativas e estado atual das mesmas. Com o uso da referida metodologia, foram realizados três estudos distintos: o primeiro, considerando um levantamento de dados em uma edificação existente que se caracteriza por sediar uma organização administrativa que faz parte do poder público, composta de 275 dependências. O segundo e o terceiro estudo tratam da aplicação em um projeto luminotécnico, no mesmo empreendimento, de duas luminárias distintas (compostas de sistemas ópticos com e sem aletas, respectivamente). A partir dessas atividades que norteiam a aplicação direta da metodologia para confecção de projetos, foram estabelecidas comparações entre os resultados obtidos e realizadas considerações sobre os índices de iluminância, de densidade de potência instalada total em iluminação e da densidade de potência em iluminação. De posse de todos os resultados obtidos em uma edificação com relevante representatividade no segmento dos prédios públicos, bem como das considerações pertinentes, podemos inferir que com o uso somente de iluminação geral, sem iluminação local é possível atingir no máximo a classificação B e que temos a possibilidade de uso dos dois tipos de luminárias adotadas sem alterar a classificação dos níveis de eficiência energética encontrados. / This study aims to assess the gains in energy efficiency through the classification levels, assigned to the installation of lighting systems, with the implementation of the proposed regulations for voluntary labeling of the level of energy efficiency in commercial buildings, of services and public in stage of approval. One contextualization of the importance of the proposed regulation was held with major systems for evaluating environmental of buildings in the international and Brazil, considering the major initiatives and current status of them. With the use of that methodology, three separate studies were conducted: first, considering a survey of data in an existing building which is hosting an administrative organization of the public power, composed of 275 dependences. The second and third studies dealing with the application in a lighting project in the same enterprise, in two different luminaries (composed of optical systems with and without fins, respectively). From these activities that guide the direct application of the methodology for preparation of projects there have been established comparisons between the results and made comments on the levels of illuminance, the density of total installed power in lighting and in lighting power density. In possession of all results obtained in a building with significant representation in the segment of public buildings, as well as the relevant considerations, we can infer that with the use only of general lighting, local lighting is not possible to achieve the maximum rating B and we have the possibility to use both types of luminaires adopted without changing the classification of levels of energy efficiency found.

O crescimento anual no número de procedimentos realizados em Medicina Nuclear se deve em primeiro lugar à vantagem de não serem invasivos. Cerca de 80% dos diagnósticos realizados em Medicina Nuclear utilizam radiofármacos preparados com tecnécio-99m devido a suas características físicas ideais, disponibilidade e baixo custo. Dentre os radiofármacos utilizados para identificar processos de infecção e inflamação estão os leucócitos marcados com tecnécio-99m, considerado o padrão ouro e o citrato de gálio (67 Ga), atualmente comercializado no Brasi. Os leucócitos marcados possuem a desvantagem da técnica laboriosa de marcação nem sempre disponível nos centros de Medicina Nuclear, enquanto o citrato de gálio (67 Ga) não é específico e possui energias desfavoráveis para a dosimetria do paciente e para a aquisição de imagens. Neste cenário, novas moléculas para diagnóstico de focos de infecção têm sido pesquisadas, particularmente envolvendo biomoléculas, como os peptídeos antimicrobianos. Nesta categoria, a Ubiquicidina na forma de um fragmento sintético (UBI 29-41) despertou o interesse de pesquisadores que estudaram a marcação com tecnécio-99m utilizando métodos direto e indireto. Um reagente liofilizado de UBI 29-41descrito na literatura demonstrou eficácia no diagnóstico de focos de infecção utilizando-se imagens cintilográficas, porém o produto não está disponível no Brasil. O objetivo deste trabalho foi estudar a marcação do fragmento de UBI 29-41 com tecnécio-99m por método direto e avaliar a utilização de soluções tampão no procedimento de marcação, de modo a flexibilizar o volume da solução de pertecnetato de sódio a ser utilizado, o que constitui aspecto de ineditismo deste trabalho. O fragmento foi radiomarcado com diferentes volumes de soluções tampão alcalinas (carbonato e fosfato) e a utilização dos tampões em substituição à solução de hidróxido de sódio possibilitou realizar a marcação com diferentes volumes da solução de pertecnetato de sódio, sem comprometimento do rendimento de marcação. Uma formulação liofilizada foi avaliada, demonstrando estabilidade por período de 12 meses, viabilizando a produção rotineira do agente de marcação. Os estudos de biodistribuição das preparações efetuadas com os diferentes tampões demonstraram que os complexos formados apresentam características gerais de biodistribuição semelhantes ao composto padrão descrito na literatura, incluindo rápido clareamento sanguíneo e alta captação renal, condizente com a eliminação do produto por esta via. Entretanto, as preparações estudadas apresentaram captação hepática maior e captação renal menor que o composto padrão. As preparações estudadas captaram no foco de infecção provocado por S.aureus e demonstraram potencial para aplicação clínica no diagnóstico em Medicina Nuclear. Como pré-requisito para a realização de estudos clínicos de um novo composto foi realizado estudo de cito e genotoxicidade, cujos resultados demonstraram a segurança das preparações estudadas. / The annual growth in the number of Nuclear Medicine procedures is directly related to the fact that they are non invasive techniques. About 80% of diagnostic procedures in Nuclear Medicine use technetium-labelled radiopharmaceuticals, because of ideal physics characteristics of this radionuclide, disponibility and low cost. Some radiopharmaceuticas employed in the diagnostic of infection and inflammation is technetium labeled leukocytes, considered the gold standard, and gallium citrate (67 Ga) that are commercialized in Brazil. The procedures for labeling leukocytes are laborious and not always aviable in the Nuclear Medicine Centers, that constitutes a disadvantage of this radiopharmaceutical. On the other hand, galium citrate (67 Ga) is not specific and its energies are not favourable for patient dosimetry and image. In this context, new molecules for diagnostic of infection have been studied, particularly biomolecules, as the antimicrobians peptides. In this category, the Ubiquicidine, as a synthetic fragment (UBI 29-41), was particularly investigated by many researchers for labeling with technetium-99m by direct and indirect methods. A liophylized kit described in the literature showed good results in the diagnostic of infection focous by scintilographic images, but this product is not aviable in Brazil. The objective of this work was to study the labeling of the UBI 29-41 fragment with technetium-99m by direct method using buffer solutions on labeling procedure, that results in a less restrictive labeling technique concerning the volume of sodium pertechnetate solution employed, that constitutes in the originality of this work. UBI fragment was labeled with different volumes of alkaline buffers (carbonate and phosphate) and the use of buffers instead sodium hydroxide solution resulted in labeling procedures that employed different volumes of sodium pertechnetate solution without loss in the labeling yield. A liophylized kit was prepared, showing stability for 12 months, that supports a rotine production of this labeling agent. The biodistribution studies using the radiopharmaceutical prepared with different buffers showed that the resulted complexes presented biodistribution characteristics similar to the standard preparation described in the literature, including fast blood clearance and renal excretion. However, the studied preparations showed higher liver uptake and lower renal uptake when compared to the standard preparation. The studied preparations presented good uptake on infection focus produced by inoculation of S.aureus and showed potential for application in clinical procedures in Nuclear Medicine. Citotoxicity and genotoxicity studies were conducted with the peptide fragment as prerequisit for future clinical studies and the results showed the security of the compound for clinical application.

Made available in DSpace on 2016-03-15T19:46:26Z (GMT). No. of bitstreams: 1 Lais Quinquio Benega.pdf: 572273 bytes, checksum: cb4463af4efd6d0ce59723b0e06b6517 (MD5) Previous issue date: 2015-03-20 / This study aims to verify the usage of referential strategies, focusing on the labeling strategy and how it works organizing opinion articles. As there is space, in this type of text, for the author to express his or her opinion or review, there is opportunity to analyze the performance of labeling as a reference and argumentative cohesion strategy. For that, the study is based on the Functionalist theory, which sees in language usage the aim of developing effective communication between speakers, taking into account the production situation, as well as the social determinants related to the production context. We tried to observe similarities and differences between the sample texts, as well as the most significant lexical choices in the context. The analysis started from the search for referential noun phrases on texts, emphasizing the phrases with a lexical core and the possible evaluative meaning present in this core. It was found that there is a large use of labeling on the chosen texts, mainly in paragraph beginnings, working as a negative evaluation term to resume situations narrated in earlier portions of the text. It was also noticed that the texts showed labeling occurrences with evaluative meaning even in the title, and this use may activate certain semantic fields converging with the proposed argument to be developed in the text. There is a difference in the use of evaluative terms in journalists‟ texts and the ones written by a professional from another area, since in these ones we can observe the choice for evaluative predications terms and labeling. This usage contributes to the conduction of the argumentation, not only to reinforce the author's position, but also to require the reader to rebuild the referent to which the label refers to. / Este trabalho tem como objetivo verificar o uso de estratégias referenciais, especialmente a rotulação, na estruturação de artigos de opinião. Por haver espaço, nesse tipo de texto, para expressão da opinião e de avaliações do autor, analisa-se a atuação da rotulação como estratégia referencial e de coesão argumentativa. Para tanto, o estudo baseia-se na teoria Funcionalista, que vê no uso da língua o objetivo de estabelecer uma comunicação efetiva entre os participantes, segundo a situação de uso, consideradas as determinações sociais ligadas ao contexto de produção. Procurou-se observar semelhanças e diferenças entre os textos da amostra, além das escolhas lexicais mais significativas no contexto. A análise partiu do levantamento dos sintagmas referenciais dos textos, com foco nos sintagmas de núcleo lexical e na possível carga avaliativa presente nesse núcleo. Verificou-se que, entre os sintagmas nominais encontrados, prevalecem rotulações em posição de início de parágrafo, que retomam situações narradas em porções anteriores do texto constituindo termo avaliativo de carga negativa. Percebeu-se, ainda, que os textos apresentavam rotulação com carga avaliativa desde o título, e esse uso pode ativar determinados campos semânticos convergentes com a proposta argumentativa a ser desenvolvida no texto. Há distinção no uso de termos avaliativos em textos de jornalistas e de profissional de outra área, visto que nestes se observa carga avaliativa nas escolhas dos termos predicações e das rotulações. Verificou-se que a rotulação realmente contribui para a condução da linha argumentativa do texto, não apenas por reforçar o posicionamento do autor, mas também por exigir do leitor que, a partir do rótulo de carga negativa, reconstrua o referente a que o rótulo se refere.