Otter populations declined drastically across many areas of England and Wales during the 1960s to 1980s. The main cause of this decline is thought to have been high concentrations of organic pollutants, in particular PCBs and dieldrin. Here we look at the health of the present day otter population, focussing on the numbers of otters, the genetic diversity of populations and investigating a possible new organic pollutant threat, polybrominated diphenyl ethers (PBDEs). A non-invasive spraint genotyping study of the otter population inhabiting the River Camel in Cornwall not only revealed that the river was capable of supporting a minimum number of 12 otters over a 9 month period, but gave insight into the ranges and genetic relationships of the individuals using the river system. A further population genetic study was carried out focussing on the River Itchen in Hampshire, a population which declined drastically to just a few isolated individuals before receiving otters through a captive breeding programme. Microsatellite genotyping of tissue samples showed the River Itchen population to be relatively diverse, indicating a successful population recovery, and haplotype analysis reveals that captive bred otters have successfully bred within the River Itchen population. However, haplotype analysis also indicates that the otters used to found the captive breeding programme were unlikely to have originated from a native British population. Concentrations of PBDEs in otters rival the high concentrations observed in many marine mammal species and are approaching the concentrations of PCBs and DDTs already observed in otters. The profile of the PBDE congeners found shows that lower congeners show relative concentrations similar to those observed in many other species of biota, with high BDE-47 dominating the profile and BDE-99 and -100 also found at significant concentrations. Otters also contain relatively high concentrations of the congeners BDE-153 and BDE-209, a trend generally typical of terrestrial top predators. In summary, the otter populations studied appear to be recovering well. However, increasing concentrations of PBDEs may cause problems for otter populations in the future.

In the 1950s the otter started to decline in numbers and distribution in Sweden and other parts of Europe. In 1972 a game law came into force, listing otter as a species that if found dead should be reported and sent to the authorities. The numbers of dead otters reported from different areas indicate population status and distribution. Between 1970 and 2012, 832 otters were sent to the authorities, the majority (66%) during the last ten years. Most were killed in traffic accidents or drowned in fishing gear. However, the main cause of the decline is believed to be environmental contaminants. Experimental data show that a PCB residue level in muscle tissue of 12 mg/kg lw causes reproductive impairment in mink (Neovison vison), suggesting reproductive problems also in the highly PCB-exposed otters in Sweden. Since the bans of PCB and DDT in the mid-1970s, concentrations of these substances in otter and fish have decreased and the otter population is increasing. Few pathological changes in otters have been found that can be related to high contaminant concentrations. However, we found a correlation between elevated PCB concentrations and alterations in bone mineral density. No relationship was seen between DDE and bone parameters. The decline of the otter coincided with the decline of grey seals (Halichoerus grypus) and white-tailed sea eagles (Haliaeetus albicilla) in Sweden, all showing decreased reproductive outcome. Reproductive success started to increase for all of them around 1990 and during the same period concentrations of PCB and DDE have decreased in these species. The body condition among female otters has increased over the study period, indicating an improved health status. However, we found a high prevalence (71%) of cysts on the spermatic duct in otters collected between 1999 and 2012, possibly caused by endocrine disrupting chemicals. Although the organochlorine concentrations in otters have decreased, otters still face many problems. New threats to the otter population in Scandinavia are the perfluorinated chemicals, including PFOS and PFOA. Results in this thesis show an increasing trend for these compounds in otters up to 2011, including some extremely high concentrations of PFOS in otters from southern Sweden.

The Swedish otter (Lutra lutra) population decreased markedly after the 1950’s and was considered to be seriously threatened for decades. Pollution from industries and other anthropogenic activities were suspected to be the major cause of the decline. The otter is a top-predator in aquatic systems and therefore particularly susceptible to bioaccumulative and toxic substances. For this reason, the species can be regarded as a valuable indicator of environmental health. Today, the Swedish otter population is increasing and has been reestablished in many areas. However, the otter is still missing in some regions of Sweden, especially in the southeast. The aim of this study was to investigate past and present levels, and temporal trends, of environmental contaminants in otters from Småland.The study is based on data of environmental contaminants in liver and muscle tissue from dead otters found in Småland during 1966-2014. I found that the perfluorinated compounds PFOS and PFOA have increased in otters since the 1970’s which is alarming and should be given special concern in future monitoring. Also, while mercury (Hg) is rather stable, selenium (Se) concentrations are decreasing, resulting in Hg exceeding Se on molar mass basis in several otters. This indicates that all of the available Se is bound to mercury, which may imply that the otters are vulnerable to additional Hg contamination. My conclusions are that the present concentrations of the studied environmental contaminants have probably not affected the otter population in general. However, otters may be exposed to toxic levels of some contaminants in certain areas that are more polluted from anthropogenic activities. This could be harmful to individual otters or their reproduction, and therefore, continued monitoring is highly relevant.

Dans les années 1950-60, un déclin des populations de loutre a été observé dans tous les pays européens. Différentes causes sont à l'origine de ce déclin : chasse, destruction de l'habitat, eutrophisation et pollution chimique. Un facteur semble occuper une place prépondérante dans cette disparition : la contamination des chaînes alimentaires par les pesticides organochlorés et les polychlorobiphényles. <br />En France, les populations de loutre ont disparu de nombreux départements, mais restent présentes dans le Massif Central et sur la côte Atlantique. Il existe également une population résiduelle en Alsace, provenant d'une campagne de réintroduction. <br />Dans le cadre de la ZABR (Zone Atelier du Bassin du Rhône) et en collaboration avec le CORA (Centre Ornithologique Rhône-Alpes), nous avons réalisé une étude sur le département de la Drôme afin d'évaluer le statut des populations de loutre sur ce département. En parallèle, une étude écotoxicologique de cette rivière a été menée, avec analyse des polluants bioccumulatifs (PCBs, organochlorés et métaux) le long de la chaîne alimentaire. Une enquête sociologique a permis d'évaluer l'état de connaissance et la perception de cet animal par les usagers de la rivière (pêcheurs, chasseurs, kayakistes...). <br />La loutre est-elle présente sur la Drôme ? Ce milieu est-il apte à la survie de cet animal ? Quel est l'état de pollution de cette rivière ? Quelles comparaisons est-il possible de réaliser avec les populations ardéchoises et alsaciennes ? Voici les principales questions auxquelles nous avons tenté de répondre.

Diss. (sammanfattning) Uppsala : Univ., 2007. / Härtill 5 uppsatser. Med sammanfattning på svenska.

In this thesis, molecular genetic methods were used to study a threatened species, the Eurasian otter. Estimates of population size and population dynamics parameters were obtained, the genetic effects of a restocking program was evaluated, and a population viability analysis was conducted to assess which demographic parameters are most important for the future viability of an otter population. Many of the studies were based on noninvasive genetic sampling of faeces. In the genetic evaluation of the restocking program, it was found that the released otters had contributed to subsequent generations. However, the effects were to a large degree limited to the near surroundings of the release areas. Comparison of two census methods, snow-tracking and noninvasive genetic census based on faeces, showed that approximately only half of the otters detected with the genetic census were found with the snow-tracking census. It is recommended to combine these two methods to obtain the most reliable estimates of population size. A short-term study on population dynamics in otters showed that apparent survival was higher in females than in males and that the rate of addition was also high and likely influenced by migration. The population viability analysis incorporated both genetics and demography and revealed that survival to first reproduction was the most crucial demographic parameter affecting the viability of the study population. This result suggests that conservation efforts should be focused on protocols that enhance the survival prospects of young females. Environmental stochasticity was also found to have large effects on the probability of extinction of this population.

Indirect signs of species presence (e.g., tracks, scats, hairs) are frequently used to detect target species in occupancy, presence/absence, and other wildlife studies. Indirect signs are often more efficient than direct observation of elusive animals, making such signs well suited for long-term and broad-scale monitoring programs. However, error associated with misidentification of indirect signs can be high, and should be measured if meaningful inferences about population parameters are to be made. This study addressed the need for systematic approaches to estimate and minimize variation due to observer error in identifying indirect signs. I reanalyzed data from 4 replicates of a presence/absence survey of northern river otters (Lontra canadensis) that had been conducted by Texas Parks and Wildlife Department (1996-2003). Sixteen observers had recorded tracks at sample points under bridges (n = 250) distributed throughout 27 counties in the Piney-Woods ecoregion of east Texas. My objectives were to 1) determine if observers were a source of bias in the survey, 2) estimate the proportion of error associated with track identification skill, and 3) evaluate the use of an international certification procedure that measured observer tracking skill. The null hypothesis that observers had no effect on the variation in reported sign was rejected. Indeed, binary logistic regression tests indicated that observers were significantly associated with variation in reported track presence. Observers were not randomly distributed among bridge sites, and therefore were significantly correlated with 4 habitat variables that may have influenced heterogeneity in otter occupancy and probability of detection (watershed, vegetation-type, water-type, bridge-area). On average, experienced observers (n = 7) misidentified 44% of otter tracks, with a range of 0% to 100% correct detection. Also, 13% of the tracks of species determined to be 'otter-like' were misidentified as belonging to an otter. During the certification procedure, participants misidentified the tracks of 12 species as otter. Inaccurate identification of indirect signs is a likely source of error in wildlife studies. I recommend that observer skill in identification of indirect signs be measured in order to detect and control for observer bias in wildlife monitoring.

Aquesta tesi es basa en el programa de reintroducció de la llúdriga eurasiàtica (Lutra lutra) a les conques dels rius Muga i Fluvià (Catalunya) durant la segona meitat dels 1990s. Els objectius de la tesi foren demostrar la viabilitat de la reintroducció, demostrar l'èxit de la mateixa, estudiar aspectes ecològics i etològics de l'espècie, aprofitant l'oportunitat única de gaudir d'una població "de disseny" i determinar les probabilitats de supervivència de la població a llarg termini.La reintroducció de la llúdriga a les conques dels rius Muga i Fluvià va reeixir, doncs l'àrea geogràfica ocupada efectivament es va incrementar fins a un 64% d'estacions positives a l'hivern 2001-02. La troballa de tres exemplars adults nascuts a l'àrea de reintroducció és una altra prova que valida l'èxit del programa.La densitat d'exemplars calculada a través dels censos visuals ha resultat baixa (0.04-0.11 llúdrigues/km), però s'aproxima al que hom pot esperar en els primers estadis d'una població reintroduïda, encara poc nombrosa però distribuïda en una gran àrea.La mortalitat post-alliberament va ser del 22% un any després de l'alliberament, similar o inferior a la d'altres programes de reintroducció de llúdrigues reeixits. La mortalitat va ser deguda principalment a atropellaments (56%).El patró d'activitat de les llúdrigues reintroduïdes va esdevenir principalment nocturn i crepuscular, amb una escassa activitat diürna. Les seves àrees vitals van ser del mateix ordre (34,2 km) que les calculades en d'altres estudis realitzats a Europa. La longitud mitjana de riu recorreguda per una llúdriga durant 24 hores va ser de 4,2 km per les femelles i 7,6 km pels mascles. Durant el període de radioseguiment dues femelles van criar i els seus moviments van poder ser estudiats amb deteniment. La resposta de la nova població de llúdrigues a les fluctuacions estacionals en la disponibilitat d'aigua, habitual a les regions mediterrànies, va consistir en la concentració en una àrea menor durant el període de sequera estival, a causa de l'increment de trams secs, inhabitables per la llúdriga per la manca d'aliment, fet que va provocar expansions i contraccions periòdiques en l'àrea de distribució.La persistència a llarg termini de la població reintroduïda va ser estudiada mitjançant una Anàlisi de Viabilitat Poblacional (PVA). El resultat va ser un baix risc d'extinció de la població en els propers 100 anys i la majoria dels escenaris simulats (65%) van assolir el criteri d'un mínim de 90% de probabilitat de supervivència. Del model poblacional construït es dedueix que un punt clau per assegurar la viabilitat de la població reintroduïda és la reducció de la mortalitataccidental. A l'àrea d'estudi, els atropellaments causen més del 50% de la mortalitat i aquesta pot ser reduïda mitjançant la construcció de passos de fauna, el tancament lateral d'alguns trams de carretera perillosos i el control de la velocitat en algunes vies.El projecte de reintroducció ha posat a punt un protocol per a la captura, maneig i alliberament de llúdrigues salvatges, que pot contenir informació útil per a programes similars. També ha suposat una oportunitat única d'estudiar una població dissenyada artificialment i poder comparar diversos mètodes per estimar la distribució i la densitat de poblacions de llúdrigues.Per últim, la reintroducció portada a terme a les conques dels rius Muga i Fluvià ha aconseguit crear una nova població de llúdrigues, que persisteix en el temps, que es reprodueix regularment i que es dispersa progressivament, fins i tot a noves conques fluvials. / This thesis deals with an otter reintroduction program carried out in the Muga and Fluvià basins in North-eastern Spain during the second half of the 1990s. The objectives of the thesis were to demonstrate the viability of the reintroduction, to demonstrate the success, to study ecological and behavioural aspects of the species, that have in the reintroduction an unique opportunity to dispose of a "designed" population and to determine long-term survival rates of the reintroduced population.Reintroduction of the otter in the Muga and Fluvià basins succeeded, because the geographical area occupied by the otter increased to 64% of positive otter stations in winter 2001-02. The finding of three adult otters born in the reintroduction area is further proof to support the success of the reintroduction program.Density values found through visual censuses were low (0.04-0.11 otters/km), but they approached what could be expected in the first stages of a reintroduced population, still small but extended over a wide area.Post-release mortality was 22% one year after release, similar to or lower than successful otter reintroduction programs. Mortality was due mainly to traffic (56%).The activity pattern of the reintroduced otters proved mainly nocturnal and crepuscular, with scarce diurnal activity. The ranges of the reintroduced otters were of the same order (34.2 km) as those found in other studies in Europe. Mean length of waterway used by an otter during 24 hours was 4.2 km for females and 7.6 km for males. During radiotracking two females bred and their movements could be followed intensively. The response of the reintroduced otter population to high seasonal fluctuations in water availability, typical in Mediterranean regions, consisted of concentration in a smaller area during summer droughts, due to the increase of dry stretches which were uninhabitable for the otter because of lack of food, and so caused periodical expansions and contractions in the otter population's range.Long-term persistence of the reintroduced population was studied through a Population Viability Analysis (PVA). The result was low risk of extinction in the next 100 years, with most scenarios (65%) meeting the criterion of a minimum of 90% probability of survival. Population modeling highlighted the importance of preventing road kills, which cause more than 50% of otter mortality, through the construction of fauna passages, the fencing of some dangerous road stretches and the use of speed restrictions.The Girona Reintroduction Otter Project tuned a protocol for trapping, handling and releasing wild otters that can provide useful information for similar programs. It also represented a unique opportunity to study an artificially designed population and to compare several methods of estimating otter distribution and density.Finally, the reintroduction carried out in the Muga and Fluvià basins has achieved the creation of a new otter population, that persists over time, reproduces regularly and is gradually dispersing, even to new river basins.

This study aimed to evaluate otter predation on stocked trout and grayling. The diet composition of piscivorous Eurasian otters (Lutra lutra) was studied on the stream Chotýšanka in central Bohemia using spraint (otter faeces) analysis during one winter period (90 days). Stocking took place mostly in the second half of 2005. Around 5000 hatchery-reared brown trouts (Salmo trutta m. fario) of size about 10 cm, 600 graylings (Thymallus thymallus) of the same size and 480 rainbow trouts (Oncorhynchus mykiss) of size about 30 cm were stocked into the stream. Spraints were collected in December 2005 and January 2006. We found and identified 1531 fish in the spraints. The primary food source of otters was fish (almost 85 % of biomass). The main fish prey was small - sized, below 20 g in weight and 13 cm in length. The bulk of otter's diet consisted of less - valued species, especially the gudgeon (Gobio gobio). Other frequently consumed fish species were the European chub (Squalius cephalus), the common carp (Cyprinus carpio), the European perch (Perca fluviatilis), the brown trout and the rainbow trout. Consumption of stocked brown trout was 1,80 %, while cosumption of stocked rainbow trout was 10,90 % of biomass. Otters in this area consumed about 723 - 1446 brown trouts and 72 - 144 rainbow trouts...