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Thesis advisor: William Stanwood / The textual analysis of season four, episode one of Mad Men entitled “Public Relations” study the productions values in terms of feminist motifs. By using social codes developed by Leed-Hurwitz and Barthes’ five systems of meaning in semiotics, observations are made about the elements of the mise-en-scène in relation to the gender roles present in the narrative. If certain codes persist, it is assumed that these repetitive observations reflect the deliberate composition of the mise-en-scènce in order to reinforce feminist motifs. The results reveal that the production values in the mise-en-scène indeed reinforce feminist motifs in Mad Men, the most comprehensive motif being the male gender as the norm. The messages communicated by this motif include the idea that masculinity is defined by sex and that a women’s worth is defined by masculinity. / Thesis (BA) — Boston College, 2011. / Submitted to: Boston College. College of Arts and Sciences. / Discipline: Communication Honors Program. / Discipline: Communication.

CEZAR, Henrique Machado. Estudo fitoquímico dos talos de Croton velutinus baill e análise dos componentes do óleo essencial de Ocimum basilicum var. purpurascens BENTH e de seus derivados por espectroscopia ordenada por difusão (DOSY). 2016. 160 f. Dissertação (Mestrado em Química)-Universidade Federal do Ceará, Fortaleza, 2016. / Submitted by Aline Mendes (alinemendes.ufc@gmail.com) on 2017-01-20T20:53:42Z No. of bitstreams: 1 2016_dis_hmcezar.pdf: 13002046 bytes, checksum: e5aaa91c1849e9c1fdc95a126cb6a497 (MD5) / Approved for entry into archive by Jairo Viana (jairo@ufc.br) on 2017-01-23T20:29:39Z (GMT) No. of bitstreams: 1 2016_dis_hmcezar.pdf: 13002046 bytes, checksum: e5aaa91c1849e9c1fdc95a126cb6a497 (MD5) / Made available in DSpace on 2017-01-23T20:29:39Z (GMT). No. of bitstreams: 1 2016_dis_hmcezar.pdf: 13002046 bytes, checksum: e5aaa91c1849e9c1fdc95a126cb6a497 (MD5) Previous issue date: 2016 / Croton velutinus Baill is a plant belonging to the family Euphorbiaceae found in the “cadeia do espinhaço”, that comprises the States of Minas Gerais and Bahia, occurring in rocky fields and sandy soils. Several species of Croton have been studied due to different therapeutics applications. There is no report about the phytochemical study of C. velutinus found in the literature. The hexane and ethanolic extracts of stalks of C. velutinus were subjected to chromatographic procedures, resulting in isolation of two flavonoids, Casticin and 5-hydroxi-3,6,7,4’-tetrametoxyflavone, two sesquiterpenes (6S,7S) 7,11-dihydroxi-bisabol-2-en-4-one and Delobanone and a inedited diterpene clerodane, velutinoic acid. The extracts of Croton velutinus were subjected to assays of larvicidal, antioxidant activities, acetylcholinesterase enzyme inhibition and angiotensin I converting enzyme inhibitors. The second part of this work, consisted in essential oil analysis of Ocimum basilicum var. purpurascens BENTH, plant known like alfavaca roxa by GC-MS and DOSY. The major components in the essential oil were identified as eugenol (65,2%) and β-caryophyllene (23,4%). The resolution of eugenol and β-caryophyllene was performed by Matriz-assisted DOSY (MAD) using the non-ionic surfactant Brij 78 as matrix. Were prepared eugenol derivatives: eugenyl acetate, cis and trans-isoeugenol, for to be analyzed by MAD in binary and ternary mixtures containing vanillin like one of components. The diffusion coeficients of eugenol and β-caryophyllene in the essential oil vary considerably with the change of solvent of CDCl3 to the mixture DMSO-d6:D2O (1:1) with ∆EC 7,27% to ∆EC 89,12% separation, respectively, whereas the binary mixture of this compounds vary of ∆EC 9,39% to 98,33%. The eugenol and vanillin 10 mM mixture showed the variation of ∆EC 35,5% in presence of Brij 78. The larvicidal activity of essential oil, eugenol and their derivatives were obtained from 3rd stage larvae of mosquito Aedes aegypti, and the essential oil showed the most efficient. The structural characterization of the isolated compounds was achieved by spectroscopy methods (MS, IR and NMR 1H e 13C, including one and two dimensional techniques) and by comparison with literature dates. / Croton velutinus Baill é uma planta pertencente à família Euphorbiaceae encontrada na cadeia do espinhaço, região que compreende os estados de Minas Gerais e Bahia, ocorrendo em campos rupestres e solo arenoso. Várias espécies de Croton têm sido estudadas devido às suas diferentes aplicações terapêuticas. A espécie C. velutinus ainda não apresenta estudos fitoquímicos descritos na literatura, o que motivou a investigação química e farmacológica desta planta. Os extratos hexânico e etanólico dos talos de C. velutinus foram submetidos a procedimentos cromatográficos resultando no isolamento de dois flavonoides Casticina e 5-hidroxi-3,6,7,4’-tetrametoxiflavona, dois sesquiterpenos, (6S,7S) 7,11-diidroxi-bisabol-2-en-4-ona e delobanona, e um diterpeno clerodano inédito na literatura, o ácido velutinóico. Os extratos de Croton velutinus foram submetidos a ensaios de atividades larvicida, antioxidante, inibição da enzima acetilcolinesterase e da enzima conversora da angiotensina I. A segunda parte deste trabalho consistiu na análise do óleo essencial de Ocimum basilicum var. purpurascens BENTH, planta conhecida como alfavaca roxa, por CG-EM e DOSY. Os componentes majoritários identificados no óleo essencial foram eugenol (65,2%) e β-cariofileno (23,4%), os quais foram isolados e analisados por DOSY assistida por matriz (MAD) utilizando o surfactante não iônico Brij 78 como matriz. Foram preparados os derivados do eugenol: acetato de eugenila, cis e trans-isoeugenol, para serem analisados por MAD em misturas binárias e ternárias contendo vanilina como um dos componentes. Os coeficientes de difusão do eugenol e β-cariofileno no óleo essencial variaram consideravelmente com a mudança do solvente de CDCl3 para a mistura DMSO-d6:D2O (1:1) com separação de ∆EC de 7,27% para ∆EC 89,12%, respectivamente, enquanto a mistura binária destes compostos variou de ∆EC 9,39% para 98,33%. A mistura de eugenol e vanilina a 10 mM mostrou separação de ∆EC de 35,5% na presença de 40 mM de Brij 78. As atividades larvicidas do óleo essencial, eugenol e dos derivados foram obtidas frente às larvas no 3º estágio do mosquito Aedes aegypti, sendo o óleo essencial o mais eficiente. As substâncias químicas isoladas tiveram suas estruturas elucidadas por métodos espectroscópicos (EM, IV e RMN 1H e 13C, incluindo técnicas uni e bidimensionais) além de comparação com dados da literatura.

Divided into three substantial chapters, this dissertation centres on the interconnections of madness and nonsense. In particular, the introduction analyzes theories on nonsense literature, and the binaric logics that separately structure the discursive fields of these two phenomena. In this section, there is also a deconstruction of the problematics of analyzing nonsense as a literary technique without the influence of mad studies. The first chapter then moves to take on the figure of the Western serial killer, and the hermeneutic projects that the media takes on when reporting on his “senseless” crimes. Arguing that the labelling of the serial killer’s crimes as “nonsensical” demonstrates a particular aesthetic that works to associate madness with danger, disease, fear, and hatred within the public imaginary, this dissertation offers an analysis through a reading of Lynn Crosbie’s Paul’s Case. In the final chapter, this dissertation employs Margaret Atwood’s Surfacing and Emma Donoghue’s The Wonder to consider nonsensical madness within the context of feminine subjectivity. Considering autotheory as a feminist hermeneutic practice then leads into the author’s own experiences as a mad person. This dissertation aims to consider how to engage with nonsensically mad feminine texts, and the ethics of the hermeneutics of mad reading-projects. / Dissertation / Doctor of Philosophy (PhD) / Why do we deem certain groups of people nonsensical? Why is nonsense considered the ‘mother-tongue’ of madness? This dissertation attempts to unfold some of the dimensions of these questions through an historical taxonomy of madness and nonsense as separate yet connected phenomena; an analysis of the media and popular categorization of the Western serial killer; and feminine people deemed ‘mad’ via hysteria. Using their own experiences of madness, the author asks how we can possibly read mad articulations when they are often chalked up to nonsense, as well as the sociopolitical implications of this configuration of nonsense and madness.

Drawing on the theoretical influences of Mad and Disability Studies; philosophical conceptualizations of epistemic injustice (Fricker, 2007), ethical loneliness (Stauffer, 2015), and psycho-emotional disablism (Reeve, 2012; Thomas, 1999; 2007); disability/service user/feminist ethics; a decade of Mad Movement community organizing; as well as autobiographical illustrations and empirical data from two collaborative research projects, this thesis describes my efforts to live a Mad politics in the community, academy, and social work education. Central to this politics, and to the overall contribution of the thesis, is its focus on (1) the recognition and redress of affective-epistemic harms that are often ignored by legislative/social welfare approaches to in/justice; and (2) the generation and refinement of Mad knowledge/ways of knowing that respond to our own priorities as Mad people, rather than those of mental health systems. It contributes to these areas of Mad Studies theory in several ways: First, by recognizing and politicizing the often ignored affective-epistemic effects of abandonment and neglect Mad people experience from society, including loneliness, anger, resentment, distrust, low expectations of others and lack of confidence. Second, by seeking new conceptualizations (such as epistemic loneliness) and contributing to existing ones (like expectations of just treatment, psycho-emotional disablism) in order to more adequately interpret and attest to these harms and call for their redress. Third, by affirming emergent Mad moral and epistemological frameworks, especially those that manifest in the aftermath of harm and account for ontologies of knowing. Fourth, by developing Survivor/Service User Research approaches to analysis (listening for resonance, everyday forms of service user resistance, and ‘quiet’ data) that value affective engagements with data and perceive and respond to Mad onto-ethico-epistemologies in and on their own terms. Ultimately, this work calls for greater relational justice, and an expansion of what we owe each other. / Thesis / Doctor of Philosophy (PhD) / This thesis contributes to Mad theory by recording some of the things I learned while trying to survive in the world, community organizing, the academy, and social work education as a Mad person. To do so, I reflect on the existential and ethical questions I brought to my doctoral studies, the people, texts, and concepts that I found particularly good company during this time, and my Mad methods of living/doing/knowing. Three separate but interconnected articles then follow. These are about (1) moving with loneliness as a Mad student; (2) resisting unmet expectations as service user ethics, and (3) how pedagogical partnerships between students and faculty/staff can cultivate marginalized students’ confidence in their knowledge. The thesis ends with a discussion of its overall contributions to how we conceptualize the psycho-emotional harms produced through sanism/disablism and the ways we understand what Mad knowledge is and how it is generated.

Ubiquitin is an essential 76 amino acid protein which can be conjugated to lysine residues on a variety of substrates via its C-terminal diglycine motif. This conjugation allows the protein to act as a molecular tag in a range of processes, including regulation of chromatin compaction, signalling cascades and DNA repair. In addition, ubiquitin moieties are capable of forming chains through the successive conjugation to lysine residues within ubiquitin itself. One of the most well characterized functions of ubiquitin is its role in protein quality control and degradation. Tetra-ubiquitin chains, most commonly through a lysine-48 linkage, are responsible for directing proteins to the 26S proteasome for degradation. This process is of importance both in the removal of miss-folded proteins, and in the regulated destruction of specific targets, such as the cyclins. The 90kDa AAA-ATPase Cdc48/p97/VCP is an essential protein that forms a hexameric complex, which interacts with a wide variety of ubiquitinated substrates. The specificity of Cdc48 is modulated by a series of different cofactors, which together allow Cdc48 to operate in several different contexts, from removal of misfolded proteins from the ER, to regulating securin stability. The role of two Cdc48 cofactors, Ubx4 and Ubx5, was studied in an attempt to dissect their function and to determine how they may modulate the function of Cdc48. Neither protein was found to be essential, as knockouts of either were found to be viable with no major defect in growth rate. The work also describes the findings of a yeast two-hybrid screen to identify potential substrates for both cofactors. Delivery of ubiquitinated proteins to the proteasome is mediated by shuttling factors, which are able to bind to both ubiquitin and the proteasome, and hence mediate the interaction between both. The shuttling factor Dph1 binds ubiquitin via a C-terminal UBA domain, while its N-terminal UBL domain mediates its interaction with the proteasome. This work identified a novel interaction between the Sti1 domains of Dph1 and the N-terminal region of a mitochondrial localized AAA-ATPase, homologous to the Saccaromyces cerevisiae protein Msp1. In addition, cell fractionation experiments revealed the presence of Dph1 at the mitochondria. This interaction provides hints that Mlp1 may be involved in the removal of ubiquitinated proteins from the mitochondria, and their delivery to the proteasome. The thesis begins to try and attempt to identify possible substrates of this proposed mitochondria associated degradation pathway, and looks for ways in which the hypothesis may be tested.

This thesis is a study of the gender politics through musical discourse in the Mad Max series. Dystopian narratives are particularly interesting texts for study of gender because they allow for extreme hypothetical situations in worlds that are at once familiar and unfamiliar. Musical discourse in the Mad Max films both supports and complicates dominant readings of gender constructions. I consider the gender politics of the franchise, using Mad Max (1979) and Mad Max: Fury Road (2015) as case studies, and drawing on scholarship on gender in film music, feminist film theory, and Australian car culture. In analyzing this music, I consider its broader cultural connotations, including film music tropes and operatic character types. After considering these genre associations, I analyze the musical gestures for narrative content and consider how the placement of themes with images and dialogue influences that content, with attention to how these factors contribute to a gendered understanding of the character. As the first deep thematic analysis of music in the Mad Max films, my project extends existing scholarship on both onscreen performance and gender categorizations that include musical forces resistant to strict binary categorization. My analysis of gendered musical discourse emphasizes the power of inquiry about gender in film music to clarify, enrich, and complicate texts.

Disputats, Stockholms universitet, 2007. / Findes også på internet (PDF-format): http://www.diva-portal.org/diva/getDocument?urn_nbn_se_su_diva-7028-2__fulltext.pdf. I publikationen: ISSN 1104-1625-141. Med litteraturhenvisninger.

There is an increasing interest in the operas of Georg Frideric Handel, both from a scholarly perspective, and that of the modern, professional opera company. Producers of Handelian opera have moved away from productions similar to those staged in Halle, Germany, in the 1920s, which featured vastly reduced recitative and stripped the da capo aria to a single statement of the 'A' section. Modern productions have restored Handel's musical text, and in addition have attempted to recreate the original dramatic conditions and ethos of the work. The problem faced by the Halle producers still exists, however. How does the modern producer satisfy the expectations of the modern audience, while remaining faithful to the intention of the composer and the original production. This paper will investigate a possible approach to staging Handelian opera, with specific reference to the 'Mad Scene' from Handel's opera Orlando. Included in this examination will be a discussion of eighteenth-century British staging practices. These elements will be considered in the light of stage design and scenic practices of the period.

Adenine deaminase (ADE) catalyzes the conversion of adenine to hypoxanthine. Mechanistic characterization of ADE from Escherichia coli was performed along with biophysical studies. The structure of ADE was solved from A. tumefaciens. The structure, along with the biochemical and biophysical characterization, enabled the elucidation of the mechanism of the deaminase reaction of ADE. Elucidation of the origin of the oxygenation reactions within ADE led to the discovery of a promiscuous catalase reaction. The diiron ADE from all tested bacterial species exhibited this unusual reaction, along with the generation of superoxide and hydroxyl radicals, the latter being responsible for the oxygenation of the protein. The residues that were identified to be oxygenated were primarily the metal binding residues implying the origin of this reaction was the binuclear iron center. A group of bacterial enzymes that are co-localized in the same genomic operon as ADE but of unknown function were identified. The enzyme Bh0637 from Bacillus halodurans, a representative member of this group of enzymes was characterized. This enzyme was shown to preferentially catalyze the deamination of epigenetic base, N-6-methyadenine. Lastly, gram-negative bacteria have a highly conserved phn operon composed of 14 genes to break the C-P bond of inert alkylphosphonates. The genes phnGHIJKLM are absolutely critical for this activity. We discovered that methylphosphonate reacts first with MgATP to form alpha-D-ribose-1-methylphosphonate-5-triphosphate (RPnTP) and adenine by the action of PhnI, PhnG, PhnH and PhnL. PhnI by itself was shown to perform a novel nucleosidase reaction converting MgATP to ribose-5-triphosphate and adenine. The triphosphate moiety of RPnTP is then hydrolyzed to pyrophosphate and alpha-D-ribose-1-methylphosphonate-5-phosphate (PRPn) by PhnM. The carbon-phosphorus bond of PRPn is subsequently cleaved via a radical-based reaction to alpha-D-ribose-1,2-cyclic-phosphate-5-phosphate (PRcP) and methane in the presence of S-adenosyl-L-methionine by PhnJ.