Hlatikulu Vlei, situated in the foothills of the Natal Drakensberg, is one of the priority wetlands in KwaZulu-Natal, occupying an area of 733 Hectares. The importance of Hlatikulu Vlei lies in its functions to store water, regulate stream flow and attenuate floods, as well as to provide suitable habitat for wildlife and grazing for livestock. Hlatikulu Vlei is a threatened system and has suffered the effects of human mismanagement. Forty-nine percent of the vlei has been classified as disturbed or destroyed, mainly due to the construction of two large dams and past drainage of vlei to facilitate pasture planting. The effects of grazing and fire on the plant communities has been considerably less. Vegetation communities at Hlatikulu Vlei have similarities with those at Ntabamhlope Vlei. The main plant communities present at Hlatikulu Vlei are: vlei grassland, sege-meadows, bulrushes and reedswamp. Species compositions of the mixed sedge and grass sedge-meadow community have a notably higher species diversity than similar communities sampled at Ntabamhlope Vlei and the mires at Highmoor. Soil type and moisture content are shown to be the most significant environmental factors determining the distribution of plant communities and species within the vlei. A wetland re-establishment and rehabilitation programme in the Hlatikulu Crane and Wetland Sanctuary has been effective in allowing many wetland plants to become reestablished. The sanctuary communities bear greater similarity to the sedge and rush sedge-meadow community, than the mixed sedge and grass sedge-meadow communities that were originally present. This is also reflected in the seed bank. All three Southern African crane species (Blue, Wattled and Crowned Crane) and fourteen species of waterfowl have been recorded in the Hlatikulu Crane and Wetland Sanctuary since the wetland rehabilitation programme. The waterfowl play a role in the dispersal of seeds into the sanctuary, particularly those of Schoenoplectus decipiens and Eleocharis dregeana and are in part responsible for the return of certain wetland plants to the sanctuary. The flooding of soils, the fluctuating water level and the soil type related to hummocks and to channels are shown to be responsible for the location of Cyperus denudatus, Arundinella nepalensis and Aristida junciformis in differing positions in the channels and on the hummocks and are also responsible for the maintenance and functioning of the hummocks and channels. Seed banks on the hummocks are similar to seed banks in the channels, however the extant vegetation on the hummocks is distinctly different to that in the channels. Certain species represented in the channel seed bank are being excluded from surviving to maturity. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 1996.

Increased residential and commercial development threatens wetland functions, particularly near urban centers. This research explores the values and attitudes of residents concerning wetlands, wetland restoration, and development near Fort Wayne and Huntington, Indiana, the location of a large wetland restoration project. Key Informant interviews were conducted to contextualize issues on wetlands, stormwater management, development, and community involvement. Interviews results were used to generate a random sample, multi-county mail survey. Results show that residents find protecting groundwater, green space, and access to clean drinking water very important. Environmental concern is high regarding flooding, development, and water quality. KI’s underestimate resident’s awareness of water quality issues. A proposed model of significant factors in explaining resident’s attitudes towards wetlands includes whether or not they are a farmer, wetland knowledge, well-being, and positive attitudes towards green space and development. This research is unique because the respondents have exposure to wetland areas on a regular basis. Wetlands are highly appreciated and are an important part of the community. There is a fine balance of property rights and pro-conservation attitudes among residents. Future research can explore whatconservation specifically means to people and how their views on property rights shape that meaning. Results from this research can aid management decisions that can be either beneficial or detrimental to the wetland and those who depend on it for a variety of needs. / Department of Natural Resources and Environmental Management

Sulfate- and Fe(III)-reducing, and methanogenic prokaryotes (SRP, FRP, MGP) are key players in metabolic pathways involved in anaerobic biodegradation processes. Understanding the metabolic activity of these microbes in environments can enhance microbe-mediated processes such as oil spill bioremediation and methane biogas production. In this study, anaerobic microbial activities in Deepwater Horizon oil spill-impacted salt marsh sediments, and in methanogenic coal bed production water enrichment cultures amended with trace elements (TE), were elucidated by employing an approach combining methods in molecular biology and geochemistry. In situ metabolic activity of SRP, FRP and MGP were monitored seasonally and metabolically-active communities were identified in oil-impacted sediments using quantitative real time Reverse Transcription -PCR and clone library analysis of key functional genes: Dissimilatory (bi)sulfite reductase (dsrAB), Geobactereceae-specific citrate synthase (gltA), methyl coenzyme M reductase (mcrA), and benzyl succinate synthase (bssA). In situ application of montmorillonite clay was assessed for its potential at accelerating PHC degradation by stimulating microbial activities. Levels of dsrA, gltA and bssA transcripts suggested that PHC-oxidizing SRP are more active in summer while FRP are more active in winter, indicating their activities linked to the seasonal changes of redox potential and vegetation. BssA gene expression peaked in winter, and was highest at more highly oil-impacted sites. Expression of all genes was higher in clay-amended sites. bssA transcript level and Fe(II) production were highest in clay-amended microcosm. Total petroleum hydrocarbon (TPH) levels were lower in oil and clay-amended microcosm incubation than one with oil only amendment, suggesting enhanced TPH degradation by clay amendment. Pyrosequencing analysis 16S rRNA gene in clay-amended microcosms demonstrated the highest percentage abundance of groups closely related to known anaerobic aromatic degraders. Levels of mcrA transcripts correlated with methane production rates in TE-amended coal bed production water enrichments. The findings of the present study clearly support the advantage of gene expression analyses for estimating microbial activity. To the best of our knowledge, this is the first in situ study which employs key functional gene markers as molecular proxies for metabolic activity and diversity assessments in anaerobic oil-contaminated salt marsh sediment and also elucidates clay-enhanced in situ TPH degradation.

S'han estudiat els efectes dels factors ambientals sobre el perífiton dels sistemes lenític fluctuants del aiguamolls de l'Empordà. L'estudi s'ha realitzat als tres nivells d'integració: nivell d'ecosistema considerant el rol del perífiton envers els altres productors primaris; a nivell de comunitat, estudiant la composició específica de les diatomees i a nivell de població estudiant la plasticitat fenotípica d'una espècie de diatomea (Nitzschia frustulum).A nivell d'ecosistema s'observa que els factors que afavoreixen el predomini dels diferents tipus de productors primaris (perífiton, fitoplàncton i macròfits) són la renovació i el grau d'eutròfia de l'aigua. A nivell de comunitat els factors determinants en la composició i distribució de les espècies de diatomees són els gradients confinament-inundació així com la productivitat del sistema. En funció d'aquest factors s'han establert 5 associacions de diatomees. A nivell de població es demostra que tant la salinitat, com la relació N : P a l'aigua com el moviment de l'aigua afecten la morfologia i ultraestructura de la valva de N. frustulum. De forma interessant s'observa que la salinitat, considerada com a factor individual, afecta N. frustulum a nivell poblacional provocant-li modificacions en la morfologia de la valva, per en canvi, no afecta a nivell de comunitat, ja que totes les espècies de diatomees presents en ambients de salinitat fluctuant són eurihalines. / The effects of environmental conditions on periphytic of lentic and fluctuating waters (Empordà wetlands) at the three different levels of organisation were studied: at ecosystem level, considering the role of periphyton among the primary producers; at community level, analysing the periphytic diatom species composition; and at population level studying the phenotypic plasticity of a selected diatom species (Nitzschia frustulum). The factors favouring the predominance of different primary productors (periphyton, phytoplankton and macrophytes) were the turnover and eutrophy of the water. At the community level, we observed that the main factors affecting diatom species composition and distribution are the gradients confinement-flooding and productivity of the system. Based on these factors 5 diatom assemblages have been distinguished. At the population level, the results of the present thesis proved that the morphology and ultrastructure of N. frustulum is affected by salinity, N : P ratio and movement of the water. Interestingly the results showed that salinity, as a single environmental factor, has effect at N. frustulum population level resulting in phenotypic plasticity while there is no effect of salinity at diatom community level since in such environments with fluctuating salinity all the species present are euryhaline.

This thesis details the hydrodynamic research conducted at Delta Marsh as part of the Restoring the Tradition marsh rehabilitation project. Research has indicated that the hydraulic and hydrologic controls on the marsh can have considerable impacts on its ecological function, but the impacts of these controls had not previously been studied. Field hydrography and two-dimensional numerical modelling (using MIKE 21) provided insight into many aspects of the physical behaviour of Delta Marsh. Eighty five percent of the inflow to Delta Marsh from Lake Manitoba passes through Clandeboye Channel, and these discharge signals propagate as far west as Cadham Bay. Inflow to the marsh disperses quickly, and accounts for a small fraction of the water that exits the marsh during subsequent outflow. Thus, Portage Diversion water that enters the marsh through the lake can remain there even if there is a net loss in marsh volume over the season. Wind friction across Lake Manitoba has the greatest impact on short-term fluctuations in marsh volume and on the composition of marsh water, followed by the Portage Diversion and the natural inflows to Lake Manitoba. Expansions to flood diversion infrastructure will considerably impact the composition of Delta Marsh waters. Three methods of wetland discharge estimation were developed and tested. The most promising method was the regressed slope Manning method (RSMM), which estimates two-directional channel discharge as a function of the water surface elevations at both ends of a channel. When used in conjunction with the velocity index method, the RSMM can multiply the amount of reliable discharge data collected per research dollar. Thanks to its simple formulation, the RSMM is likely applicable outside of wetland settings, as well. / February 2016

S’inscrivant dans la continuité des études menées sur le Nord-Pas-de-Calais, cette thèse vise à mettre en avant l’apparition et le développement du paysage septentrional dans la peinture française au XIXe siècle. Si des études de ce genre ont déjà été réalisées pour la Normandie et la Bretagne, il n’en est pas de même pour le Nord-Pas-de-Calais. Ce travail inédit, mis en relation avec un contexte historique -visant pour l’essentiel à étudier et cerner les déplacements des artistes au sein de la région-, culturel et artistique dense, permettra de comprendre dans quelles circonstances et de quelles manières se développent ces réalisations. L’étude vise également à cerner et définir les motifs emblématiques de la région tels les beffrois, les moulins, les espaces dunaires, les marais … L’étude reposera un corpus d’œuvres, formant un catalogue, représentatif du paysage septentrional au XIXe siècle. / In addition to the many studies of the Nord-Pas-de-Calais, this thesis aims to highlight the emergence and development of the northern landscape in French paintings of the nineteenth century. Even though there have already been some studies done regarding the Normandy and Brittany regions, it is not the case for the Nord-Pas-de-Calais. This never published before research combined with the historical context aims to study and identify the movement of artists within the region whose artistic culture is dense. This will help us understand when and how were developed these achievements. The study also aims to identify and define the emblematic patterns of the region such as belfries, mills, dune areas, marshes ... The study will use a collection of works, forming a catalogue representative of the northern landscape in the nineteenth century.

Sediment accretion and elevation change in tidal forests, and the corresponding ability of these wetlands to keep pace with sea-level rise (SLR), represent data gaps in our understanding of wetland sustainability. Surface Elevation Tables and marker horizons were installed in three mature tidal forests and a restored tidal marsh, allowing us to measure elevation change, accretion, and subsidence. Additionally, we measured predictor variables to test for their significance in explaining accretion and elevation change rates. Mean accretion at our sites was 11.67 +/- 3.01 mm yr-1 and mean elevation change was -20.22 +/- 8.10 mm yr-1, suggesting subsidence occurring beneath the sites. Processes contributing to accretion and elevation change at our sites may be driven by hydrologic patterns. Comparing our elevation trends with SLR trends suggests that our study sites may not keep pace with SLR. However, we may be observing short-term oscillations that do not indicate true long-term trends.

Understanding species-habitat relationships for endangered species is critical for their conservation. However, many studies have limited value for conservation because they fail to account for habitat associations at multiple spatial scales, anthropogenic variables, and imperfect detection. We addressed these three limitations by developing models for an endangered wetland bird, Yuma Ridgway's rail (Rallus obsoletus yumanensis), that examined how the spatial scale of environmental variables, inclusion of anthropogenic disturbance variables, and accounting for imperfect detection in validation data influenced model performance. These models identified associations between environmental variables and occupancy. We used bird survey and spatial environmental data at 2473 locations throughout the species' U.S. range to create and validate occupancy models and produce predictive maps of occupancy. We compared habitat-based models at three spatial scales (100, 224, and 500 m radii buffers) with and without anthropogenic disturbance variables using validation data adjusted for imperfect detection and an unadjusted validation dataset that ignored imperfect detection. The inclusion of anthropogenic disturbance variables improved the performance of habitat models at all three spatial scales, and the 224-m-scale model performed best. All models exhibited greater predictive ability when imperfect detection was incorporated into validation data. Yuma Ridgway's rail occupancy was negatively associated with ephemeral and slow-moving riverine features and high-intensity anthropogenic development, and positively associated with emergent vegetation, agriculture, and low-intensity development. Our modeling approach accounts for common limitations in modeling species-habitat relationships and creating predictive maps of occupancy probability and, therefore, provides a useful framework for other species.

The purpose of the study was to provide further knowledge about if the total amount of solar radiation during the marsh fritillary’s (Euphydryas aurinia) period for larvae development (April 15th – May 15th) and/or flying period (June 1st – June 30th) influenced the average of larvae colonies in the autumn at 12 sites in Dalarna County. Among increased temperatures, global climate models also predict an increased amount of solar radiation and a decrease in cloudiness in northern Europe, as a result of the climate changes. The marsh fritillary has declined rapidly, especially in the Northern of Europe and are in Sweden red-listed as vulnerable (VU). The marsh fritillary is a univoltine species who are dependent on open, sunny, and moist semi-natural grasslands with the hostplant devil’s bit (Succisa pratensis). The data over solar radiation was received from SMHI and had been measured in Borlänge over the last 14 years. The data over the number of larvae colonies was received from Länsstyrelsen Dalarna and had been measured over the last 15 years. The linear regression analysis for both the period of larvae development (p=0,58) and the whole period (p=0,07) indicates that during those periods the solar radiation did not influence the average of larvae colonies. However, the total amount of solar radiation during the flying period (p=0,02) influence the average of larvae colonies during the autumn.

Thesis (Ph. D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), February 2002. / Vita. / Includes bibliographical references (leaves 153-161). / I used direct measurements of nitrogen gas (N₂ fluxes and a ¹⁵N stable isotope tracer to determine the contribution of denitrification to salt marsh sediment N cycling. Denitrification in salt marsh tidal creekbottoms is a major sink for groundwater nitrate of terrestrial origin. I studied creekbottom denitrification by direct measurements of N₂ fluxes in closed chambers against a low-N₂ background. I undertook experiments and simulation modeling of sediment N₂ fluxes in closed chambers to optimize the key experimental parameters of this approach. Denitrification in these sediments was driven by the degradation of labile organic matter pools which are depleted during long incubations. Sediment thickness was the most important parameter controlling the required incubation time. Errors of up to 13% with gas headspaces and 80% with water headspaces resulted from headspace N2 accumulation and the resulting collapse of the sediment-water diffusion gradient. These errors could be eliminated by using headspaces of sufficient thickness. Headspace flushing to reduce ammonium accumulation did not affect denitrification rates, but caused transient disturbance of N₂ flux rates. Direct measurements of 0₂, C0₂, N₂, and inorganic N fluxes from the sediments of a salt marsh tidal creek were made in order to examine the interaction of denitrification with the carbon, oxygen, and N cycles. Organic carbon concentration and lability were the primary controls on metabolic rates. C0₂/N flux ratios averaged 6.1, indicating respiration driven by algal biomass. / (cont.) Allochthonous denitrification accounted for 39% of total sediment denitrification (2.7 mol N m⁻² yr⁻¹). 46% of remineralized ammonium was denitrified, while the contribution of autochthonous denitrification to 0₂ and C0₂ fluxes was 18% and 10%, respectively. A ¹⁵N-ammonium tracer was used to study competition between plants and nitrifying bacteria for remineralized ammonium. In undisturbed sediments of Spartina alterniflora, plant uptake out-competed nitrification-denitrification, with plant uptake accounting for 66% of remineralized ammonium during the growing season. Under N fertilization (15.5 mol m⁻² yr⁻¹), both plant N uptake and denitrification increased, but denitrification dominated, accounting for 72% of the available N. When plant uptake was hydrologically suppressed, nitrification-denitrification was stimulated by the excess N, shifting the competitive balance toward denitrification. / by Michael Robert Hamersley. / Ph.D.