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Quantifying the effect of inbreeding on the growth and yield of Mozambique Tilapia (Oreochromis mossambicus) over three generations of repeated full-sib matingAkinoshun, Kolawole M. 12 1900 (has links)
Thesis (MSc)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: The effects of acute inbreeding on growth performance, yield and occurrence of deformity traits were studied in experimental full-sibling inbred populations of Mozambique tilapia Oreochromis mossambicus at three levels of inbreeding coefficients, namely F = 0.000, F = 0.250 and F = 0.375. The base population with F = 0.000 was established through the crossing of two geographically separated and genetically unrelated farm stocks. At each generation, the inbreeding depression indicated by body weight (BW), standard length (SL), specific growth rate (SGR) and yield were highly significant, but no linear relationship was found between level of inbreeding and inbreeding depression. Both condition factor (K) and the number of observed deformities appears not to be significantly affected by inbreeding at all three levels.
Over all, the average inbreeding depression at F = 0.250 and F = 0.375 respectively was found to be 46.5 percent and 46.6 percent for body weight (BW); 18.2 percent and 18.0 percent for standard length (SL); 21.8 percent and 20.3 percent for specific growth rate (SGR) and 5.752 percent and 8.940 percent for flesh yield. The outbred Control group differed significantly (P<0.05) from the six inbred family groups in terms of body weight (BW), standard length (SL), specific growth rate (SGR) and yield at all levels of inbreeding studied (F = 0.000, F = 0.250 and F = 0.375).
Average inbreeding depression for body weight (BW) amongst the six inbred families ranged from 39.6 to 54.2 percent at F = 0.250 (in Gen 2) and 45.6 to 47.3 percent at F = 0.375 (in Gen 3). The inbreeding depression coefficient for body weight (BW) per 10% increase in F, amongst the six inbred families, ranged from 15.9 to 21.7 percent at F = 0.250 and from 12.2 to 12.6 percent at F = 0.375.
Average inbreeding depression for standard length (SL) amongst the six inbred families ranged from 14.0 to 22.3 percent at F = 0.250 and from 17.2 to 18.4 percent at F = 0.375.
The inbreeding depression coefficient for standard length (SL) amongst the six inbred families ranged from 5.6 to 8.9 percent at F = 0.250 and from 4.6 to 4.9 percent at F = 0.375.
Average inbreeding depression for specific growth rate (SGR) amongst the six inbred families ranged from 17.9 to 27.9 percent at F = 0.250 and from 16.7 to 27.2 percent at F = 0.375. The inbreeding depression coefficient amongst the six inbred families ranged from 7.2 to 11.2 percent at F = 0.250 and from 4.5 to 7.3 percent at F = 0.375.
Average inbreeding depression for yield amongst the six inbred families ranged from 0.4 to 7.7 percent at F = 0.250 and from 8.5 to 10.2 percent at F = 0.375. The inbreeding depression coefficient for yield amongst the six inbred families ranged between 0.2 and 3.1 percent at F = 0.250 and from 2.3 to 2.7 percent at F = 0.375.
The condition factor (K) of the six inbred families showed no significant differences to the Control (P > 0.05) at all levels of inbreeding with K-values ranging from 1.42 to 2.85.
The occurrence of morphological deformities in all seven family groups including the Control showed no noticeable trend, with a random, nonlinear occurrence of fluctuating asymmetry observed at different inbreeding levels in O. mossambicus.
This study demonstrates that inbreeding has a significant negative effect on the production traits of Oreochromis mossambicus, especially growth. Results from this study emphasize the need to create awareness amongst small scale farmers of the importance of preventing uncontrolled inbreeding in production systems, as well as to monitor inbreeding levels during the process of dissemination of improved fish strains to small scale fish growers in developing countries, including Africa. / AFRIKAANSE OPSOMMING: Die gevolge van akute inteling op die groei prestasie, opbrengs en voorkoms van misvorming eienskappe bestudeer in eksperimentele full-broer ingeteelde bevolkings van Mosambiek tilapia Oreochromis mossambicus op drie vlakke van inteling koëffisiënte, naamlik F = 0,000, F = 0,250 en F = 0,375. Die basis bevolking F = 0,000 gestig deur die kruising van twee geografies geskei en geneties onverwant plaas aandele. Op elke generasie, die inteling depressie aangedui deur die liggaam gewig (BW), standaard lengte (SL), spesifieke groeitempo (SGR) en opbrengs was hoogs betekenisvol, maar geen lineêre verband is gevind tussen vlak van inteling en inteling depressie. Beide toestand faktor (K) en die aantal waargeneem deformiteite verskyn om nie beduidend beïnvloed deur inteling op al drie vlakke.
Oor alles is, die gemiddelde inteling depressie by F = 0,250 en F = 0,375 onderskeidelik gevind om 46,5 persent en 46,6 persent vir die liggaam gewig (BW) wees; 18,2 persent en 18,0 persent vir standaard lengte (SL); 21,8 persent en 20,3 persent vir spesifieke groeitempo (SGR) en 5,752 persent en 8,940 persent vir vlees opbrengs. Die outbred beheer betekenisvol verskil (P <0,05) van die ses ingeteelde familie groepe in terme van die liggaam gewig (BW), standaard lengte (SL), spesifieke groeitempo (SGR) en opbrengs op alle vlakke van inteling bestudeer (F = 0,000 , F = 0,250 en F = 0,375).
Gemiddeld inteling depressie vir die liggaam gewig (BW) onder die ses ingeteelde families gewissel 39,6-54,2 persent by F = 0,250 (in Gen 2) en 45,6-47,3 persent by F = 0,375 (in Gen 3). Die inteling depressie koëffisiënt vir die liggaam gewig (BW) per 10% toename in F, onder die ses ingeteelde families, het gewissel 15,9-21,7 persent by F = 0,250 en 12,2-12,6 persent by F = 0,375.
Gemiddeld inteling depressie vir standaard lengte (SL) onder die ses ingeteelde families gewissel 14,0-22,3 persent by F = 0,250 en 17,2-18,4 persent by F = 0,375. Die inteling depressie koëffisiënt vir standaard lengte (SL) onder die ses ingeteelde families gewissel 5,6-8,9 persent by F = 0,250 en 4,6-4,9 persent by F = 0,375.
Gemiddeld inteling depressie vir spesifieke groeitempo (SGR) onder die ses ingeteelde families gewissel 17,9-27,9 persent by F = 0,250 en 16,7-27,2 persent by F = 0,375. Die inteling depressie koëffisiënt onder die ses ingeteelde families gewissel 7,2-11,2 persent by F = 0,250 en 4,5-7,3 persent by F = 0,375.
Gemiddeld inteling depressie vir opbrengs onder die ses ingeteelde families het gewissel van 0,4-7,7 persent by F = 0,250 en 8,5-10,2 persent by F = 0,375. Die inteling depressie koëffisiënt vir opbrengs onder die ses ingeteelde families gewissel tussen 0,2 en 3,1 persent by F = 0,250 en 2,3-2,7 persent by F = 0,375.
Die toestand faktor (K) van die ses ingeteelde families het geen betekenisvolle verskille op die Beheer (P> 0,05) op alle vlakke van inteling met K-waardes wissel 1,42-2,85.
Die voorkoms van morfologiese afwykings in al sewe familie groepe, insluitend die beheer het geen merkbare neiging, met 'n ewekansige, nie-lineêre voorkoms van wisselende asimmetrie waargeneem op verskillende vlakke in inteling O. mossambicus.
Hierdie studie toon dat inteling het 'n beduidende negatiewe uitwerking op die produksie-eienskappe van Oreochromis mossambicus, veral groei. Resultate van hierdie studie beklemtoon die noodsaaklikheid om bewustheid onder kleinboere van die belangrikheid van die voorkoming van onbeheerde inteling in die produksie stelsels, sowel as om te monitor inteling vlakke tydens die proses van verspreiding van verbeterde vis stamme klein skaal vis produsente in ontwikkelende lande te skep, insluitend Afrika.
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Community building for economic empowerment in rural Mozambique: An exploratory study in the Maganja da Costa DistrictSaide, Eusebio M. 12 1900 (has links)
Thesis (MPhil (Sustainable Development Planning and Management))--University of Stellenbosch, 2006. / Though the Maganja da Costa District in Mozambique has potential for the development
of natural resources, the District is neither economically self-reliant nor empowered and
is the poorest within the country. Thus, the research question set for this study is: What
are the main factors that inhibit poor people in the study area from effectively using local
resources for their livelihoods and what possible alternatives could enable them to
achieve economic empowerment? In an attempt to answer the question, the following
aspects were investigated: the systems of local resources, product and indigenous
knowledge use and management; the local mechanisms of acquiring and sharing
information, knowledge and skills; the obstacles to acquiring and sharing information,
knowledge and skills; the influence of such obstacles on the management of local
resources and livelihood strategies, as well as on the community’s organisational,
leadership and entrepreneurship capacity.
An exploratory study was conducted in the study area using the qualitative method,
involving participatory action research. A comparative literature review and field work
was conducted in order to collect the data. Raw data were collected in two phases: While
pilot research took place over 5 days, more extensive research took place over 21 days.
During the extensive research, in-depth household interviews were conducted, using
semi-structured personal interviews, focus group interviews and discussions, direct
observations and cross-checking methods employing a sample size of 101 respondents
randomly selected and 10 key informants. The Statistical Programme of Social Science
(SPSS) was used to process and analyse the raw data.
The results show that the main factors that inhibited poor people in the study area from
effectively using local resources and products for their livelihood were: i) a lack of
knowledge, skills and talents; ii) the inadequate mechanisms in place for sharing local
information, knowledge and skills; iii) the ineffective community organisation and
leadership; iv) a lack of entrepreneurship skills and capabilities; v) the inadequate
existing infrastructure, transport and trading systems; vi) a low level of partnership and
networking; vi) a disruption of socio-cultural cohesion; and vi) inadequate mechanisms
for planning, implementation and management of local development strategies,
programmes and projects by local government.
Most of the government’s development strategies in Mozambique focus on economic
growth, which does not necessarily entail the economic empowerment of poor people.
The role of traditional leadership has been neglected, which has resulted in the disruption
of traditional values and belief systems that might otherwise have positively contributed
to socio-cultural cohesion. The role that community building could play in assisting poor
people in the study area to establish common values, and to develop collective goals and
actions, should enable them to acquire and/or share information, knowledge, skills and
talents in such a way as to strengthen themselves. Such strengthening of organisational,
leadership and entrepreneurship capacities and skills could significantly contribute to
attaining economic self-reliance, poverty alleviation and sustainable development, if the
community building approach were to be adequately applied. Additional research is required in order to identify appropriate mechanisms for making further advances in
applying such an approach in rural Mozambique, especially in the study area.
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Peacebuilding in Mozambique with special reference to the UN policy on landmine removalVan Tonder, Delarey 12 1900 (has links)
Thesis (MPhil)--Stellenbosch University, 2000. / ENGLISH ABSTRACT: The end of the Cold War had a profound impact on the qualitative and quantitative nature of the
UN's peace and security agenda, representing a shift from traditional peacekeeping to a broader,
more ambitious and intrusive notion of peacekeeping. This evolution was marked by an expanded
UN engagement in a broad range of intra-state conflicts and characterised by UN undertakings
towards aspects of national political and socio-economic reconstruction including the evolution
of humanitarian action.
Responding to the expanded United Nations agenda for international peace and security and at
the request of the UN Security Council (UNSC) Boutros Boutros-Ghali prepared the conceptual
foundations of the UN's role in global peace and security in his seminal report, An Agenda for
Peace (July, 1992). The Secretary General outlined five inter-connected roles that he projected
the UN would play in the fast changing context of post-Cold War international politics, namely:
preventive diplomacy, peace enforcement, peacemaking, peacekeeping and post-conflict peacebuilding.
The UNSG described the newly defined concept of post-conflict peacebuilding as action organised
"(to) foster economic and social co-operation with the purpose of developing the social,
political and economic infrastructure to prevent future violence, and laying the foundations for a
durable peace."
With specific reference to landmines in An Agenda for Peace the UNSG stressed that peacebuilding
following civil war and internal strife must address the serious problem of landmines, which
remained scattered in present or former combat zones. The UNSG underscored that mine action
(demining) should be emphasised in terms of reference of peacekeeping operations which is
crucially important in the restoration of activity when peacebuilding is under way.
The United Nations involvement in the Mozambican peace process (1992-1995) has been interpreted
as the culmination of a major success story in wider peacekeeping in Africa under UN auspices
- a category of peace operation, which included peacemaking, peacekeeping, humanitarian
assistance, peacebuilding and electoral assistance. Mozambique's peace process has subsequently
been cited as a model UN peacekeeping operation which could be adapted to post-conflict situation
elsewhere.
Within the context of landmines as a threat to post-conflict peacebuilding as articulated by the
UNSG in An Agenda for Peace, the study focuses on how the United Nations implemented mine
action initiatives in operationalising the concept of peacebuilding in Mozambique. In this context,
the study reviews the UN operation in Mozambique (ONUMOZ) and its capacity, responsiveness
and vision in implementing mine action initiatives, both in terms of the operational requirements
of the ONUMOZ peacekeeping mission and the development oflonger-term humanitarian mine
action programmes in Mozambique. To this end, the study views the establishment of a sustainable
indigenous mine action capacity as a sine que non for post -conflict peacebuilding.
From this perspective, the study interprets the 1999 Mine Ban Treaty Prohibiting the Use, Stockpile,
Production and Transfer of Anti-Personnel Mines and on their Destruction and the rights
and obligations of Mozambique as a State Party to the Treaty as the most appropriate instrument
towards the creation of an indigenous Mozambican mine action capacity to address the long-term
effects oflandmines on post-conflict peacebuilding.
In terms of methodology the approach was historical-analytical and in essence a deductive
method of research was followed. / AFRIKAANSE OPSOMMING: Die einde van die Koue Oorlog het diepgaande verandering teweeggebring ten opsigte van die
Verenigde Nasies se vredes en sekuriteits regime ter handhawing van internasionale vrede en
sekuriteit. Hierdie periode is gekenmerk deur 'n skerp toename in intra-staatlike konflikte en
gevolglik in die kwalitatiewe en kwantitatiewe aard en omvang van Verenigde Nasies vredesoperasies
in terme van die VN Handves.
Ten einde die verantwoordelikhede van die Verenigde Nasies met betrekking tot die handhawing
van vrede en sekuriteit in die snel - veranderende konteks van die post - Koue Oorlog periode
aan te spreek, het die Sekretaris - Generaal van die Verenigde Nasies, Boutros Boutros - Gali,
in opdrag van die Veiligheidsraad die konseptuele fundering van die VN se rol verwoord in sy
pioniersverslag - Agenda vir Vrede (1992).
In sy verslag van Julie 1992 identifiseer en omskryf die Sekretaris-Generaal vyf verbandhoudende
konsepte wat sou dien as meganismes ter beslegting van internasionale konflik, naamlik
voorkomende diplomasie (preventive diplomacy), vredesingryping (peace enforcement),
maak van vrede (peacemaking), vredesoperasies (peacekeeping) en post-konflik vredeskonsolidasie
(post-conflict peacebuilding).
Die Sekretaris-Generaal het post-konflik vredeskonsolidasie omskryf as die "vestiging van
sosio-ekonomiese samewerking met die oogmerk om die sosiale, politieke en ekonomiese infrastruktuur
te ontwikkel ten einde hernude konflik te voorkom en ter grondlegging van langdurige
vrede".
Met spesifieke verwysing na die korrelasie tussen landmyne en post-konflik vredeskonsolidasie
het die Sekretaris-Generaal benadruk dat landmyne 'n bedreiging inhou vir die konsolidasie van
vrede na burgeroorlog en interne konflik, en veral binne die raamwerk van 'n VN vredesoperasie
in terme van 'n VN Veiligheidsraad mandaat.
Die VN se vredesrol in Mosambiek word allerweë beskou as een van die mees suksesvolle
VN vredesoperasies ooit. Die doel van die studie is gevolglik om ondersoek in te stel na die
toepassing van die konsep van post-konflik vredeskonsolidasie met spesifieke verwysing na
die Mosambiekse vredesproses en die rol van die Verenigde Nasies se Operasie in Mosambiek
(ONUMOZ). In die opsig fokus die studie spesifiek op die rol van ONUMOZ (1992-1995) en
suksesse en tekortkomings in sy vredesmandaat ten opsigte van die implementering van aksies
om die kort-en-langtermyn impak van landmyne in terme van post-konflik vredeskonsolidasie
in Mosambiek aan te spreek.
Vanuit hierdie konteks, vertolk die studie die Landmyn Verdrag (1999) en die totale verbod op
die aanwending, opgaar, produksie en oordrag van landmyne en die vernietiging daarvan as die
mees geskikte raamwerk waarbinne Mosambiek 'n inheemse vermoë tot stand kan bring ten
einde die langtermyn impak van landmyne op post-konflik vredeskonsolidasie effektief aan te
spreek.
Vanuit 'n metodologiese oogpunt word in hierdie studie histories analities te werk gegaan en
die benadering is beskrywend - verklarend van aard. Verder is die metode van ondersoek in
wese deduktief van aard.
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Frontier deliquescence in the texts of Mia CoutoRothwell, Phillip January 2000 (has links)
No description available.
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The USSR and Afro-Marxist regimes : the path to the Treaties of Friendship and Co-operationSimpson, Mark S. C. January 1989 (has links)
No description available.
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The influence of practical work on chemistry teaching and learning: an approach using microchemistry kits in Mozambican junior secondary schoolsMadeira, Antonio Cristo Pinto 28 May 2009 (has links)
The problem which motivated this research was the lack of chemistry practical
work in many Mozambican junior secondary schools. This problem is so critical
that quite often learners finish the three years of junior secondary school without
performing even one experiment.
In an attempt to contribute to the solution to the problem, the microchemistry kits
are introduced. For this purpose a study of the influence of kits on the teaching
and learning of chemistry in a Mozambican context was conducted.
The study involved four out of five public secondary schools in the city of Beira,
the second largest city in Mozambique. Two schools were chosen to be the
experimental group, in which chemistry was taught using the RADMASTE
microchemistry kits. Two other schools, in which chemistry lessons were taught
normally, were chosen to be the comparison group. In each school one Grade 9
class was used for the study.
Before starting the study, 18 secondary chemistry teachers of the four schools
answered a questionnaire and 163 Grade 9 learners answered another
questionnaire. These were used to determine teachers’ and learners’ opinions
about the importance and aims of practical work. Before the intervention a pretest
was administered to 181 Grade 9 learners within the four classes.
After eight weeks of intervention, the same post-test was administered to 171
learners from the same classes. A questionnaire was also administered to the
two teachers of the experimental group and another questionnaire was
administered to 86 learners from the experimental group. Both questionnaires
were used to find out teachers’ and learners’ opinions about the microchemistry
kits.
Practical work is viewed as an important method for teaching and learning
chemistry, mainly to link theory and practice or use practical work to support
theory.
There was a statistically significant difference between the pre-test and the posttest
scores in all four schools. But, learners from the experimental group
performed better than learners from the comparison group in the questions which
required conceptual understanding and in laboratory-based knowledge
questions.
The practical work also contributed to increase learners’ interest towards
chemistry.
It is recommended that the microchemistry kits be implemented in chemistry
teaching in Mozambique, both in schools with laboratories and schools without
laboratories, and that further studies should be carried out to identify effective ways of doing this.
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Framing issues of environmental security in Angola & Mozambique - the nexus of land, conflicts and sustainable livelihoods in post-conflict situationsClover, Jeanette Lee 19 May 2009 (has links)
ABSTRACT
Violent and protracted conflicts, such as those that affected Mozambique and Angola (both
countries with a Lusophone colonial heritage), have had severe consequences in terms of
wartime dislocation and destruction, especially in rural areas. Land issues per se are not
endogenously conflictive, but in post-conflict settings, the scramble for access to the assets
necessary to (re-)establish livelihoods for large numbers of people, as well as the pursuit of
land access by large-scale commercial interests who capitalise on a fluid land tenure
situation to acquire resources, may occur.
A nuanced and comparative study of Mozambique and Angola is undertaken that explores
the relationship between violence, resources and the environment. It asks two questions: i)
What accounts for the relationship between violence (evidenced in both brutal physical acts,
threats and increasing vulnerabilities) and land as a resource? ii) Are there lessons to be
learnt from these findings that are particular to countries emerging from protracted civil
wars? The thesis explores the changing discourses around the concepts of human security
and environmental security, and the pressing land issues confronting the African continent. It
highlights the complexity of issues – political, social and economic – and the necessity for a
theoretical shift away from the popular approaches towards alternative ways of
understanding the connections between the environment, violence and resources. It examines
the specific dynamics of a post-conflict environment, an area that has received little
attention, despite its potential for playing a significant role in ensuring broad-based
development and in peace-building. A modified livelihoods framework is also used to
analyse land issues on the basis that land is an element of a wider livelihoods approach with
a focus on poverty alleviation and wealth creation.
Findings mirror those of other international researchers who have found that conflicts over
land often have less to do with resource scarcity, but that “violence is more likely when
resources are in great abundance or have great economic and strategic value” (Peluso and
Watts, 2001: 5). Furthermore, findings support the calls for taking a more inclusive concept
of violence and non-violence that recognises that the outward manifestation of disputes may
not be violence in the form of civil war, but social disruptions (Liotta, 2005). The value of a
post-structuralist political ecology for analysing these various connections is demonstrated in
the research findings. It is one which does not search for ‘environmental triggers’ of violent
conflicts, but looks at the reciprocal relationship between nature and humans. Both countries
are confronting many of the land issues that are common to Africa and which suggest an
important new phase in the politics of land. In Angola land tenure and shelter are now
insecure for many in both rural and urban areas, while in both countries there is mounting
competition and conflict over land and landed resources. There are increasing threats of
exclusionary practices and land grabs, but also the more subtle, ‘non-traditional’ security
threats of the destruction and damage to livelihoods, of deepening impoverishment, evident
in "creeping vulnerabilities”. The findings of the research confirm that in dealing with both
equity and efficiency issues, and environmental sustainability and political stability, land
policies need to be well integrated into wider social, economic and environmental planning –
at various levels, local to global – to strengthen sustainable security.
vi
Land conflicts are generally contained as local-level disputes, often camouflaged by
government or suppressed. While conflict theory points to apparent triggers – differential
impacts and political mobilisation – it must be acknowledged that these tensions are more
often than not politically sustainable, as leaders justify overriding the interests of the poor in
the interests of growth. Furthermore, peace is not the default mode of society: conflicts are at
times an integral part of the transformation of land tenures systems and not necessarily
destructive in themselves. Concerns need to focus, rather, on those cases where inequity and
violence are politically sustainable, and what this means for human security. It is this issue
that is recommended for further research.
“In contrast to thinking about violent conflict, a human-centred
conceptualisation of environmental security asserts the need for
cooperation and inclusion to manage the environment for the
equal benefit of all people and future generations” (Barnett,
2001: 128).
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Foreign direct investment and worker rights : a case study of a private security multinational in Mozambique.Carvalho, Daniela Sampaio de 06 March 2009 (has links)
This article intends to contribute with the reflection upon the theories that link FDI with social and economical development. For this purpose, the meanings of the expression “human and economical development” will be briefly reviewed, and later it will be approached along the theories on the relation of FDI with development. The theories are used as a support in order to reach this article’s goal of pointing out the FDI impacts on labour conditions on the private security multinational G4S in Mozambique, thus examining the impacts of this sort of FDI towards the country’s human development.
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Coal-based linkages and development in Mozambique: a political economy perspectiveSelemane, Tomás Mário 29 January 2015 (has links)
Thesis (M.Com. (Development Theory and Policy))--University of the Witwatersrand, Faculty of Commerce, Law and Management, School of Economic and Business Sciences, 2014. / Mozambique is currently moving from an aid dependent country to mineral dependent given the
mining boom happening there thanks to the discoveries of huge reserves of coal, mineral sands
and natural gas. The country is set to become one of the world's twenty top producers of natural
gas and top ten largest producers of coal.
This research is a case study focusing on coal-based linkages that can foster broad economic
growth and development in Mozambique. Using a political economy perspective, the research
investigates the question about how the country can optimise the mining boom through coalbased
economic linkages to foster broader socio-economic development.
The research finds that under the combination of its current fiscal and mineral regime with
infrastructure problems plus the inexistence of a coal-based linkage policy, Mozambique will get
negligible benefit from the exploitation of its finite coal resources. A major overhaul of these
regimes is needed for it to make use of its coal to catalyse wider growth and development, before
it is left with little other than large holes in the ground.
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Insecticide resistance and vector status of Anopheles funestus and An. gambiae populations at a sugar estate in MozambiqueKloke, Ronald Graham 12 April 2010 (has links)
MSc (Med), Faculty of Health Sciences, University of the Witwatersrand, 2009 / Malaria is on the increase in Mozambique since 2001 and impacts primarily on
children < 5 years of age. Insecticide resistance in the malaria vector mosquitoes is on
the increase in Mozambique and Africa and is cause for serious concern. Maragra
sugar estate is situated in close proximity to the nKomati river floodplain in a rural
area in Mozambique and requires intense irrigation for cane growing and as a result
provides extensive breeding sites for An. funestus and other mosquitoes. In the areas
surrounding the estate there are two important vectors of malaria, Anopheles funestus
group and An. gambiae complex. There is intense malaria transmission in the areas
surrounding the sugar estate and the last entomological study on the vectors in the
Manhica area was done in 1998. It was becoming increasingly urgent to identify to
species level the vectors in this area and to monitor the insecticide resistance status of
these vectors. Due to leakage (theft) of insecticides and a change by the National
Malaria Control Programme (NMCP) to an insecticide to which the predominant
vector is resistant, an entomological survey was carried out in this area from January
2009 to March 2009 to ascertain by Polymerase chain reaction (PCR) what species of
malaria vectors were present inside and outside of the Maragra vector control area,
their population levels and their vectorial status in these two areas. Insecticide
resistance studies by insecticide exposure and the synergist piperonyl butoxide (pbo)
were carried out using the World Health Organisation (WHO) bioassay method on
collected An. funestus mosquitoes. This was done to establish this species resistance
status to the four classes of insecticides recommended by the WHO for malaria vector
control. The collections of An. arabiensis and An. merus that were identified were too
few to carry out insecticide resistance tests on these two species. Enzyme linked
v
immuno-sorbent assay (ELISA) tests were undertaken to establish the vectorial
capacity of Anopheles funestus and An. gambiae complex in this area. The
predominant malaria vector species in this area is An. funestus s.s., with the secondary
vector being An. arabiensis. An. funestus has a high vectorial capacity in this area and
found to have a Plasmodium falciparum sporozoite rate of 6.02%. This is an increase
in the sporozoite rate of 1.2% from 1998 when the last survey in this regard was
carried out. Coupled with this increase is an increase in the An. funestus populations
in this area since this time. One An. gambiae complex sample was found to be
positive but the species is not known as this particular sample did not amplify on
PCR. Anopheles funestus is highly resistant to synthetic pyrethroids and exhibits a
lower level of resistance to bendiocarb, a carbamate insecticide in use at Maragra
sugar estate. The synergist pbo mediates the resistance mechanism in both these
insecticides indicating that the metabolic resistance mechanism present in this
mosquito is strongly mediated by monooxygenase detoxification. The role of the
medical entomologist is increasingly necessary and important in the monitoring of this
resistance phenomenon in malaria vector mosquitoes, as is the role of the vector
control programme manager in implementing and managing vector control
programmes. The implication of cane sugar farming and its impact on vector
production and malaria transmission is discussed. Insecticide resistance and the
change by the NMCP to a synthetic pyrethroid to which the predominant vector of
malaria is resistant is discussed.
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