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A Critical Evaluation of the 2009 Niger Delta Amnesty Disarmament Demobilisation and Reintegration ProgrammeInuwa, Solomon January 2017 (has links)
Disarmament, Demobilisation and Reintegration (DDR) have become a key
component of the postconflict peacebuilding orthodoxy. Therefore, this study
evaluates the efficacy of Amnesty, Disarmament, Demobilisation and
Reintegration (ADDR) in conflict prevention and resolution using the 2009 Niger
Delta ADDR programme as a case study. The study evaluated the effectiveness
of the programme using the minimalist and maximalist framework advanced in
the DDR literature. The key findings and conclusions of the Study were that a
minimalist DDR would only achieve security stabilisation and return excombatants
to the status quo- ante society with all the pre-conflict grievances
unaddressed thereby bequeathing a high potential of relapse to violence.
Furthermore, for DDR to be an effective conflict prevention and resolution
mechanism and postconflict peacebuilding force, its conceptualisation, design
and implementation must be maximalist in nature with a transformative agenda
that aims to address the roots causes of violence.
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Constitutional Environmental Rights: Investigating their Potentials for a Sustainable Niger DeltaOdong, Nsikan-Abasi Umana 18 September 2023 (has links)
Nigeria is at a crossroad - how to balance developmental needs with environmental protection. The challenge is exacerbated because Nigeria operates a mono-economy which overwhelmingly depends on the crude oil resources from the Niger Delta for its economic survival. As a result, the protection of the Niger Delta environment has not been accorded the priority it deserves. The thesis aims to investigate the potentials of Constitutional Environmental Rights (CERs) to assist in resolving the environmental sustainability crisis in the Niger Delta.
The thesis will utilize insights developed by environmental justice scholarship as the theoretical framework to investigate the main causes of the sustainability crisis in the Niger Delta and to propose ways to tackle these environmental challenges. The thesis draws inspiration from the research carried out by David Boyd on the efficacy of CERs for environmental sustainability for its analytical framework.
Although Trans-National Corporations (TNCs) and international trade contribute to the sustainability crisis in the Niger Delta, the thesis will not focus on these. Instead, it will focus on the internal legal causes of the sustainability crisis in the Niger Delta, because the external causes of the sustainability crisis have been addressed at length by other researchers. Moreover, addressing the internal causes of the sustainability crisis could also address some of the impacts of the external causes of the sustainability crisis in the Niger Delta. As such, the thesis uses 3 of Boyd's CERs performance indices in analyzing the suitability of CERs to tackle the 3 identified major internal causes of the sustainability crisis in the Niger Delta. Specifically, Boyd's index 1 (impetus for the enactment of stronger environmental laws) could address gaps in Nigeria's environmental regulatory framework. Index 4 (improvement in the implementation and enforcement of environmental laws) could address the non-implementation and non-enforcement problems with the existing environmental regulatory framework in Nigeria. Lastly, index 6 (increased public participation in environmental governance) could address the marginalization of the Niger Delta in resource governance in Nigeria. These indices will not only help to uncover the weaknesses in Nigerian laws and their enforcement but will also identify potential barriers to CERs within the current legal and policy architecture and suggest solutions on how CERs would be implemented if recognized in Nigeria to avoid these barriers.
The main contribution of the thesis is a detailed case study of how CERs may work in Nigeria to tackle the environmental crisis in the Niger Delta, and a detailed and specific analysis of what would be required in terms of domestic political, structural and legal change to ensure that CERs could contribute to the sustainability of the Niger Delta as much as they have in other countries.
The research makes specific recommendations for changes to Nigerian law, policy and institutions, such as adoption of CERs in the enforceable part of the Constitution, ownership and control by federating units of natural resources found in their territories, and elimination of barriers to access to justice. This would come about through strategically crafted constitutional provisions and laws to address the underlying factors that would limit the effectiveness of CERs in Nigeria. The thesis argues that addressing these fundamentals and constitutionalizing environmental rights will lead to improved environmental outcomes for the Niger Delta.
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Valorization of guayule and soy biomass through pretreatment, enzyme production and enzymatic hydrolysisIslam, S. M. Mahfuzul 23 May 2018 (has links)
No description available.
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Lixiviation fongique des résidus miniers par A. niger et P. simplicissimumOuattara, Abibata 13 April 2018 (has links)
La présente étude vise d'une part, à fournir et définir les paramètres de base nécessaires au développement de la lixiviation fongique des métaux et d'autre part, à améliorer sa rentabilité économique par l'utilisation d'une source de substrat économique. Pour ce faire, différents essais en flacons ont été réalisés en laboratoire. En premier lieu, ces essais ont permis de mettre au point une procédure de lixiviation, notamment le choix d'une méthode de lixiviation adaptée à la lixiviation de deux résidus miniers d'origines différentes. En second lieu, le perméat de lactosérum a été sélectionné parmi 7 résidus agroalimentaires comme une nouvelle source de substrat pour remplacer le sucrose habituellement utilisé au laboratoire lors des essais de lixiviation fongique. Par la suite, l'influence de divers paramètres de production des acides organiques (qui sont les agents de lixiviation) et de lixiviation des métaux sur la solubilisation de 6 métaux lourds (Cu, Fe, Mn, Ni, Pb et Zn) a été évaluée. L'influence de la distribution géochimique des métaux a été étudiée en particulier. Les résultats obtenus montrent que la lixiviation fongique offre la possibilité de récupérer à des concentrations commercialement attractives les métaux lourds retenus dans les importantes quantités de résidus miniers rejetés par l'industrie minière. Elle se révèle ainsi comme une biotechnologie prometteuse qui s'intègre au développement durable non seulement en préservant l'environnement et la santé publique des conséquences néfastes de la présence des métaux lourds, mais aussi en permettant la valorisation de ces derniers comme une source secondaire de matières premières, ainsi que celle du perméat de lactosérum comme une source de substrat pour la biosynthèse des acides organiques.
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Economie des guerres civiles : analyse économique des conflits armés intra-étatiques en Afrique Occidentale / Economics of civil wars : analysis of the intra-state armed conflicts in West AfricaAbba Gana, Souleymane 12 November 2010 (has links)
Les guerres civiles en Afrique sont multiples, désastreuses et multicausales. Cependant les analyses privilégient fréquemment les causes identitaires liées à l‟ethnique ou à des faits historiques. Il est proposé ici, une analyse différente par une approche économique. Si au sens de l‟économie classique ou néoclassique, les ressources naturelles sont une dotation constituant un avantage absolu ou comparatif, en Afrique, elles contribuent à alimenter les guerres intra-étatiques pénalisant ainsi tout effort de développement. L‟exploitation d‟uranium au Niger, loin de permettre une performance économique grâce aux revenus qu‟elle génère, représente a contrario une double source de « malédiction » : Conforme au « syndrome hollandais » accompagné de la médiocrité économique qui le caractérise mais constitue également un enjeu autour duquel se fonde la rébellion armée. / The civil wars in Africa are multiple, disastrous and multicausal. However analyses privilege frequently the identical causes connected to the ethnic or to the historic facts. It is proposed here, a different analysis by an economic approach. If in the sense of the classic or neo-classic economy, natural resources are an endowment constituting an absolute or comparative advantage, in Africa, they contribute to feed the intra-state wars punishing any effort of development. The exploitation of uranium in Niger, far from allowing an economic performance thanks to the income which it generates, represents a double source of "curse": in compliance with the "dutch disease" accompanied with the economic mediocrity which characterizes it but also constitutes a stake in which bases the armed rebellion.
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La patrimonialisation d'institutions pastorales peules au Mali : le ƴaaral et le degal (Delta intérieur du Niger)Leblon, Anaïs 13 December 2011 (has links)
Le classement des institutions pastorales peules du yaaral et du degal (Delta intérieur du Niger) à la liste des chefs-d’œuvre du patrimoine oral et immatériel de l’humanité de l’UNESCO est au fondement d’une interrogation sur les modalités de l’appropriation et de l’application des politiques internationales du patrimoine culturel immatériel. Dans un contexte de décentralisation politique et de transformations des sociétés agro-pastorales, le croisement des enjeux contemporains du pastoralisme transhumant à l’application du programme de sauvegarde conçu selon les nouvelles normes patrimoniales de la protection de la diversité culturelle, de l’implication des populations et du développement durable, alimente une réflexion sur les pratiques politiques de la tradition et de l’appartenance. Les interactions entre ce système politique et symbolique d’usage du passé et les représentations et pratiques ordinaires, parfois conflictuelles, du bien classé, révèlent les relations de pouvoir relatives à la gestion des identités et à l’exploitation du foncier entre l’État malien et divers acteurs. Le croisement et le recyclage mutuel de stéréotypes sur le pastoralisme peul mobilisés dans des stratégies contradictoires à des échelles locales, nationales et globales produisent une scène originale des fêtes de transhumance. Une lecture continuiste entre tradition et patrimoine dans laquelle celui-ci serait une forme extravertie et institutionnalisée de la tradition rend compte du caractère historique des processus de patrimonialisation et de leur inscription dans une histoire longue marquée par des usages et des reformulations dynamiques des symboles culturels. / The proclamation of the yaaral and degal pastoral institutions (Inner Niger Delta) on the UNESCO’s list of “masterpieces of the oral and intangible heritage of humanity” is the basis for the study of the forms of appropriation and implementation of international intangible heritage policies. In a context of political decentralization and transformation of agro-pastoral societies, the intersection of contemporary challenges of transhumant pastoralism with the application of a safeguarding program developed according to the new patrimonial standards for the protection of cultural diversity, the involvement of populations and sustainable development, fuels consideration of political practices of tradition and belonging. The interactions between this political and symbolic system of use of the past and ordinary representations and practices of the listed item, sometimes conflicting, reveal power relationships relative to the management of identities and land exploitation, between the Malian State and miscellaneous actors. The intersection and mutual recycling of stereotypes about fulɓe pastoralism mobilized in contradictory strategies at local, national and global scales, produce an original stage for transhumance festivals. Considering the continuum between tradition and heritage, in which heritage would be an extroverted and institutionalized form of tradition, highlights the historical nature of heritagization processes and their inclusion in a long history marked by uses and dynamic reformulations of cultural symbols.
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Erosion en milieu sableux cultivé au Niger : dynamique actuelle et passée en liaison avec la pression anthropique et les changements climatiques / Erosion in cultivated field in Niger : actual and recent dynamics in liaison with human pressure and climatic changesAbdourhamane Touré, Amadou 07 June 2011 (has links)
Les écosystèmes sahéliens, et tout particulièrement ceux qui se développent sur des sols sableux, réagissent très vite et très intensément aux moindres changements climatiques. Dans cette zone semi-aride, l’érosion éolienne qui affecte essentiellement les surfaces cultivées met en péril l’exploitation durable des ressources en terre. Par ailleurs, l’explosion démographique de ces dernières décennies et les grandes crises climatiques à l’origine des sécheresses du vingtième siècle ont eu d’importantes répercussions sur le milieu. Pour gérer au mieux ce milieu fragile, il importe d’abord de bien comprendre son fonctionnement. Ce travail de thèse s’est donc inscrit dans le programme de recherche AMMA (Analyse Multidisciplinaire de la Mousson africaine) et se situe au coeur du programme CORUS2 (Coopération pour la Recherche Universitaire et Scientifique) concernant essentiellement l’étude de la dynamique éolienne des sols sableux cultivés de la région de Niamey. La stratégie suivie au cours de cette étude a été de s’appuyer sur un important dispositif de mesures expérimentales pour comprendre au mieux et quantifier le fonctionnement actuel du milieu essentiellement vis-à-vis de l’érosion éolienne. Ces mesures expérimentales ont permis d’interpréter les changements que nous avons pu mettre en évidence sur un ensemble de terroirs proches de Niamey qui constituent les bassins versants de deux lacs permanents récents : Bangou Bi et Bangou Kirey (13,51° N – 2,21° E). Les enregistrements sédimentaires recueillis dans ces lacs ont ensuite été analysés en regard de l’évolution de leur bassin versant. Au cours de cette étude nous avons mis en évidence le rôle majeur des résidus de culture dans les champs traditionnels et montré que même à des taux de recouvrement extrêmement bas, ils constituent une protection pour les sols. Cependant leur efficacité chute en deçà d’un seuil de 2% et l’érosion éolienne déplace alors des quantités considérables de terres (130 t/an en moyenne) et participe activement à l’encroûtement des sols. Il est probable qu’à la faveur combinée de la mise en culture de l’ensemble des versant avant 1975 et de la sécheresse de 1985, un tel processus d’érosion et d’encroûtement s’est mis en place expliquant ainsi la dégradation considérable du milieu que nous avons mise en évidence entre 1975 et 2009 à partir d’enquêtes auprès des population et par la cartographie diachronique des petits bassins versant étudiés. A partir de l’étude des sédiments collectés dans les lacs, nous avons pu proposer un modèle de mise en place de ces sédiments qui suggère une intensification de l’érosion à partir du milieu des années 80. / Sahelian ecosystems, especially those installed on sandy soils react very quickly and intensely to small climatic changes. In this semi-arid area, wind erosion which occurs mainly on cultivated millet fields and is threatening the sustainable use of land resources. Furthermore, the rapid population growth and the severe droughts of the twentieth century have had important impacts on ecosystems. To better manage this fragile environment, it is important first to understand how it works. This thesis was included in the framework of the AMMA project (African Monsoon Multidisciplinary Analyses) and was in the heart of a CORUS 2 (Coopération pour la Recherche Universitaire et Scientifique) program which aimed to study wind erosion of cultivated sandy soils in the region of Niamey. The strategy followed in this study was based on detailed experimental measurements aiming to precisely quantify and understand current environment dynamics and more precisely, wind erosion dynamics. The results of experimental measurements were used to explain the changes we observed on small catchments of two permanent lakes near Niamey: Bangou Bi and Bangou Kirey (13.51 ° N - 2, 21 ° E). The sediment cores collected in these lakes were then analyzed in light of their catchments changes during the last century. In this study, we highlighted the major role of crop residues cover in traditionally cultivated field against wind erosion. Even at extremely low cover rate, they provide efficient protection for soils. However, below a threshold of 2%, their efficiency dramatically decrease and wind erosion caused large amount of soil losses (in average 130 t per year) and is actively involved in soil crusting. It is likely that this mechanism (wind erosion due very low crop residue cover and soil crusting) occurred by the middle of eighties due to the combined effects of generalized soil cultivation and sever drought of 1985. These processes explained the large degradation of the lakes watersheds we observed from surveys and diachronic mapping between 1975 and 2009. With sediment core analyses, we proposed a model of sedimentation in the Niamey lakes. This model suggests increasing erosion since the 1980’s. From the study of sediments core collected in lakes, we proposed a model explaining sedimentation that suggests an intensification of erosion from the mid 80s.
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Desenvolvimento de sistema de esterilização por plasma: eficácia inerente e comparativa com óxido de etileno / Development of a plasma sterilization system: inherent and comparative efficacy with ethylene oxideSilva, Juliano de Morais Ferreira 10 August 2006 (has links)
Estudos envolvendo o emprego de novas técnicas de esterilização têm apresentado nítido crescimento nos últimos anos como alternativa aos processos convencionais. A grande possibilidade consiste no emprego do plasma como agente de esterilização. Essa tecnologia tem considerável potencial para desenvolver o meio mais eficiente e seguro de esterilização de artigos termossensíveis com ênfase para a indústria farmacêutica e médica e até mesmo em outras áreas industriais. O objetivo deste trabalho foi investigar a influência de alguns parâmetros de processos por plasma e correlacionar sua eficácia com processos por óxido de etileno. Neste trabalho, estudos foram realizados empregando tecnologia de esterilização por plasma usando reator Reactive Ion Etching (RIE). Os valores aplicados de potências de rádio-frequência, a 13,56 MHz foram 25 W, 50 W, 100 W e 150 W. Os gases testados foram oxigênio puro e misturas de oxigênio e peróxido de hidrogênio (190/10, 180/20 e 160/40 sccm) e fluxo constante de 200 sccm, pressão de 0,100 torr e temperatura abaixo de 60°C. Esterilizador por óxido de etileno foi empregado a 450 mg/L (Oxyfume 2002®), 55°C, 60% de umidade e -0,65 e 0,60 kgf/cm2 de pressão. Os indicadores biológicos empregados foram constituídos de esporos de Bacillus subtilis var. niger ATCC 9372, inoculados em lamínulas de vidro de 18 x 18 mm e discos de papel de 13 mm de diâmetro em uma carga de 2,0 x 107 UFC/suporte. Os tempos de exposição aos processos por plasma foram de 3 a 120 minutos. Reduções progressivas da contagem inicial de microrganismos foram observadas nos valores D: 215,91,55,55,9,19 e 2,91 minutos para processos por plasma com oxigênio puro, a 25 W, 50 W, 100 W e 150 W, respectivamente. Misturas de oxigênio e peróxido de hidrogênio apresentaram os seguintes valores D: 190/10 sccm (6,41 min), 180/20 sccm (6,47 min) e 160/40 sccm (4,02 min), a 100 W e 190/10 sccm (1,47 min), 180/20 sccm (3,11 min) e 160/40 sccm (1,94 min), a 150 W. Processos empregando óxido de etileno apresentaram valor D de 2,86 minutos. Análises por Microscopia Eletrônica de Varredura demonstraram danos causados ao córtex dos esporos. Sistema empregando plasma como principal agente de esterilização apresentou-se efetivo em desafios com indicadores biológicos. Os processos por plasma provaram ser a mais apropriada tecnologia de esterilização de materias termossensíveis e com grande potencial para substituir os métodos convencionais de esterilização em futuro próximo. / Studies involving new sterilization techniques have increased in the past few years as alternatives to conventional processes. The great possibility consists in the use of plasma as sterilization agent. This technology has the enormous potential to develop a more efficient and safer means of sterilization at thermo-sensitive matters, focusing the pharmaceutical and medical industry - even though it can be applied to other industrial areas. The aim of the present study was to investigate the influence at some parameters of plasma processes and correlate the effectiveness plasma with ethylene oxide sterilizer. In this work, studies were performed taking into account a plasma sterilization technology using a Reactive Ion Etching (RIE) reactor. Power was applied at 13.56 MHz using a 6 inch diameter electrode. The gases tested were pure oxygen and oxygen-hydrogen peroxide mixtures (190/10, 180/20, and 160/40 sccm), and gas flow held constant 200 sccm, pressure at 0.100 torr and radio-frequency power at 25 W, 50 W, 100 W, and 150 W and temperature below 60°C. Ethylene oxide sterilizer were performed using 450 mg/L (Oxyfume 2002®) at 55°C, 60% humidity and -0.65 and 0.60 kgf/cm2 pressure. The biological indicator used was Bacillus subtilis var. niger ATCC 9372, witch was inoculated in glass carries (18 x 18 mm) and paper discs (13 mm diameter) in a load of 2.0 x 107 CFU/support. The exposition times were 3 to 120 minutes. Progressive reductions of the initial microbial count could be observed in the D values witch were 215.91, 55.55, 9.19, and 2.91 minutes for pure oxygen plasma at 25 W, 50 W, 100 W and 150 W, respectively. Oxygen-hydrogen peroxide mixtures plasma showed D values: 190/10 sccm (6.41 min), 180/20 sccm (6.47 min) and 160/40 sccm (4.02 min) at 100 W and 190/10 sccm (1.47 min), 180/20 sccm (3.11 min) and 160/40 sccm (1.94 min) at 150 W. Ethylene oxide processes showed D value to 2.86 minutes. Scanning Electron Microscopy analyses showed some damage on the spore cortex. Processes using plasma as main sterilization agent are presented effective in challenge with biological indicators. The plasma proved to be the most appropriate sterilization technology in thermosensitive matters and to have a great potential to replace conventional sterilization methods in the near future.
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Estudos genéticos e moleculares da produção de celulases e hemicelulases em Aspergillus nidulans e Aspergillus niger / The genetic and molecular studies of cellulase and hemicellulase production in Aspergillus nidulans and Aspergillus niger.Gouvêa, Paula Fagundes de 31 July 2013 (has links)
O mundo se depara atualmente com a perspectiva de um significativo aumento na demanda por etanol combustível. O bagaço de cana está entre os maiores subprodutos agroindustriais no Brasil, sendo uma das alternativas na utilização para a produção do etanol de segunda geração. A degradação do bagaço de cana requer a ação de muitas enzimas diferentes que são reguladas transcripcionalmente. Considerando-se que o custo de celulases e hemicelulases contribuem substancialmente no preço do bioetanol, novos estudos visando o entendimento da eficiência e produtividade de celulases são de grande importância. Para entender como melhorar coquetéis de enzimas que podem hidrolizar o bagaço de cana-de-açúcar pré-tratado, uitlizou-se um experimento de genômica para investigar-se quais genes e vias são transcripcionalmente moduladas durante o crescimento de A. niger em bagaço de cana-de-açúcar explodido. Neste trabalho foram identificados genes que codificam celulases e hemicelulases com aumento da expresão durante o crescimento em bagaço de cana-de-açúcar explodido. Foi também realizada a determinação do acúmulo de mRNA de diversos genes que codificam transportadores para verificar se estes eram induzidos por xilose e por depedência de glicose. Foram identificados 18 genes que corresponde a 58% de celulases preditas em A. niger e 21 genes que correponde a 58% de hemicelulases preditas em A. niger os quias foram altamente expressos durante o crescimento em bagaço de cana-de-açúcar explodido. Foi investigado também o papel central realizado pelas proteínas quinases e fosfatases não essenciais (NPKs e NPPs, respectivamente) quando em presença de celulose como fonte de carbono, no sensoriamento do estado energético e na subsequente via de sinalização no fungo filamentoso modelo Aspergillus nidulans. O estudo com A. nidulans identificou 11 quinases e 7 fosfatases não essências, NPKs e NPPs, respectivamente, envolvidas na produção de celulases e em alguns casos, na produção também de hemicelulases. O envolvimento destas NPKs identificadas na resposta induzida por avicel e na desrepressão foram acessados pela análise do transcriptoma da cepa selvagem e por microscopia de fluorescência através da cepa de fusão CreA::GFP expressa no selvagem e no background dos mutantes de NPKs. A ausência das quinases snfA e schA reduziu dramaticamente a resposta transcricional induzida por celulose incluindo a expressão de enzimas hidrolíticas e transportadores, enquanto que a ausência de snfA resultou em uma quase completa modulação gênica induzida por celulose. O mecanismo pelo qual essas duas quinases controlam a transcrição gênica foi identificado, onde os dois mutantes de quinases foram capazes de desbloquear o CreA mediante a repressão catabólica do carbono (CCR), sob condições de desrepressão, como em baixa presença de carbono ou crescimento em celulose. Desta forma, este trabalho abriu novas possibilidades para o entendimento da sacarificação do bagaço de cana-de-açúcar por hidrolases de A. niger e para a construção de coquetéis de enzimas mais eficientes para a obtenção do etanol de segunda geração. Também possibilitou a identificação de muitas quinases e fosfatases envolvidas no sensoriamento do carbono e do estado energético, as quais demonstraram papéis sobrespostos e distintos de snfA e schA na regulação da desrepressão de CreA e na produção de enzimas hidrolíticas em A. nidulans. / The world today is faced with the prospect of a significant increase in demand for fuel ethanol. Sugarcane bagasse is among the largest agro-industrial by-products in Brazil, one of the alternatives in use for the production of second generation ethanol. Degradation of sugarcane bagasse requires the action of many different enzymes which are transcriptionally regulated. Considering that the costs of cellulases and hemicellulases contribute substantially to the price of bioethanol, new studies aimed at understanding and improving cellulase efficiency and productivity are of paramount importance. To understand how to improve enzymatic cocktails that can hydrolyze pretreated sugarcane bagasse, we used a genomics approach to investigate which genes and pathways are transcriptionally modulated during growth of A. niger on steam-exploded sugarcane bagasse. We also sought to determine whether the mRNA accumulation of several steam-exploded sugarcane bagasseinduced genes encoding putative transporters is induced by xylose and dependent on glucose. We identified 18 genes that corresponds to 58% of A. niger predicted cellulases and 21 genes that correspond to 58% of A. niger predicted hemicellulases, that were highly expressed during growth on sugarcane bagasse. The central role performed by nonessential protein kinases (NPK) and phosphatases (NPP) when grown on cellulose as a sole carbon source, in the sensing energetic status and the subsequent signalling pathways was assessed in the model filamentous fungus Aspergillus nidulans. This study identified multiple kinases and phosphatases (NPKs and NPPs, respectively) involved in the sensing of carbon or energetic status, while demonstrating the overlapping and distinct roles of snfA and schA in the regulation of CreA derepression and hydrolytic enzyme production in A.nidulans. The involvement of the identified NPKs in avicel-induced responses and CreA derepression was assessed by genome-wide transcriptomics and fluorescent microscopy of a CreA::GFP fusion proteinexpressed in the wild-type and NPK-deficient mutant backgrounds. The absence of either the schA or snfA kinase dramatically reduced cellulose-induced transcriptional responses including the expression of hydrolytic enzymes and transporters, while the absence snfA resulted in a near complete loss of wild-typecellulose-induced gene modulation. The mechanism by which these two NPKs controlled gene transcription was identified, as neither of NPK-deficient mutants were able to unlock CreA-mediated carbon catabolite repression, under derepressing conditions, such as carbon starvation or growth on cellulose. Our presently reported work opens new possibilities for understanding sugarcane biomass saccharification by A. niger hydrolases and for the construction of more efficient enzymatic cocktails for second-generation bioethanol. This work also enable the identification of multiple kinases and phosphatases involved in the sensing of carbon or energetic status, while demonstrating the overlapping and distinct roles of snfA and schA in the regulation of CreA derepression and hydrolytic enzyme production in A.nidulans.
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Impact du forçage pluviométrique sur les inondations du fleuve Niger à Niamey : Etude à partir de données satellitaires et in-situ / Impact of rainfall forcing on the floods of Niger river in Niamey : study based on satellite and in-situ dataCassé, Claire 26 November 2015 (has links)
Depuis le développement des mesures satellites de nombreuses missions spatiales sont dédiées au suivi de l'atmosphère et de la surface terrestre. Ces travaux de thèse s'inscrivent dans le cadre de la mission Megha-Tropiques dédiée au cycle de l'eau et de l'énergie en zone tropicale. L'objectif est d'évaluer le potentiel des estimations de précipitation par satellite pour des applications hydrologiques en zone tropicale. Les Tropiques réunissent les plus grands fleuves du globe, mais ne bénéficient pas de réseaux d'observation in-situ denses et continus permettant une gestion intégrée efficace de la ressource et des systèmes d'alertes. Les estimations des précipitations issues des systèmes d'observation satellite offrent une alternative pour ces bassins peu ou pas instrumentés et souvent exposés aux extrêmes climatiques. C'est le cas du fleuve Niger, qui a subi une grande variabilité climatique depuis les années 1950, mais aussi d'importants changements environnementaux et hydrologiques. Depuis les années 2000, le Niger moyen connaît une recrudescence des inondations pendant la période de crue Rouge (engendrée par ses affluents sahéliens pendant la mousson). A Niamey, des niveaux record de hauteur d'eau et de période d'inondation ont été enregistrés en 2003, 2010, 2012 et 2013, engendrant de nombreuses pertes humaines et matérielles. Ces travaux analysent l'influence du forçage pluviométrique sur les inondations liées à la crue Rouge à Niamey. Une gamme de produits pluviométriques (in situ et satellite) et la modélisation hydrologique (ISBA-TRIP) sont combinés pour étudier : (i) l'apport des produits satellite pour diagnostiquer la crue Rouge récente, (ii) l'impact des caractéristiques des produits et de leurs incertitudes sur les simulations et enfin (iii) l'évaluation du rôle des précipitations, face aux changements de conditions de surface, dans l'évolution de la crue Rouge à Niamey depuis les années 1950. L'étude a mis en évidence l'impact des caractéristiques des estimations des précipitations (cumul, intensité et distribution spatio-temporelle) sur la modélisation hydrologique et le potentiel des produits satellites pour le suivi des inondations. Les caractéristiques des précipitations se propageant dans la modélisation, la détection des inondations est plus efficace avec une approche relative à chaque produit plutôt qu'avec un seuil absolu. Ainsi des produits présentant des biais peuvent être envisagés pour la simulation hydrologique et la détection des inondations. Le nouveau produit TAPEER de la mission MT présente un fort potentiel hydrologique, en 2012 et pour la zone d'étude. D'autre part, l'étude de la propagation de l'erreur associée à ces précipitations a mis en évidence, la nécessité de déterminer la structure du champ d'erreur pour l'utilisation d'une telle information en hydrologie. Enfin la modélisation a été utilisée comme levier pour décomposer les sensibilités de la crue Rouge aux variations des précipitations et des conditions de surface. Pour simuler les changements hydrologiques entre les périodes 1953-1982 et 1983-2012, les changements d'occupation du sol et d'aire de drainage doivent être pris en compte. Puis les variations des précipitations peuvent expliquer les changements majeurs décennaux et annuels entre les années 1983 et 2012. / Since the development of satellite based remote sensing in the 1970s, many missions have been dedicated to monitoring the terrestrial atmosphere and surfaces. Some of these satellites are dedicated to the Tropics with specific orbits. Megha-Tropiques (MT) is devoted to the water and energy cycle in the tropical atmosphere and provides an enhanced sampling for rainfall estimation in the tropical region. This PhD work was initiated within MT hydro-meteorological activities, with the objective of assessing the hydrological potential of satellite rainfall products in the Tropics. The world most important rivers lay in tropical areas where the in situ observation networks are deficient. Alternative information is therefore needed for water resource management and alert systems. The present work focuses on the Niger River a basin which has undergone drastic climatic variations leading to disasters such as droughts and floods. Since 1950, the Niger has been through 3 main climatic periods: a wet period (1950-1960), a long and intense drought period (1970-1980) and since 1990 a partial recovery of the rainfall. These climatic variations and the anthropic pressure, have modified the hydrological behaviour of the basin. Since 2000, the middle Niger River has been hit by an increase of floods hazards during the so-called Red flood period. In Niamey city, the highest river levels and the longest flooded period were recorded in 2003, 2010, 2012 and 2013, leading to heavy casualties and property damage. This study combines hydrological modelling and a variety of rainfall estimation products (satellite and in-situ) to meet several objectives: (i) the simulation of the Niamey Red flood and the detection of floods (during the recent period 2000-2013) (ii) the study of the propagation of satellite rainfall errors in hydrological modelling (iii) the evaluation of the role of rainfall variability, and surface conditions, in the changes of the Red flood in Niamey since the 50s. The global model ISBA-TRIP, is run with a resolution of 0.5° and 3h, and several rainfall products were used as forcing. Products derived from gauges (KRIG, CPC), pure satellite products (TAPEER, 3B42RT, CMORPH, PERSIANN) and mixed satellite products adjusted by rain gauges (3B42v7, RFE2, PERSIANN-CDR). This work confirms the hydrological potential of satellite rainfall products and proposes an original approach to overcome their biases. It highlights the need for documenting the errors associated with the rainfall products and the error structure. Finally, the hydrological modelling results since the 1950s have given a new understanding of the relative role of rainfall and surface conditions in the drastic increase of flood risk in Niamey.
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