A citricultura brasileira está fundamentada predominantemente sobre apenas um cultivar porta-enxerto, o limoeiro Cravo, e explora poucos cultivares copa, de uma maneira geral. Desse modo, o setor citrícola fica limitado na abertura de suas fronteiras de exportação para frutas frescas por estar baseado em um pequeno número de cultivares. Além disso, ao redor de 80% dos plantios estão reunidos em uma região compreendida por quase 600 mil ha, de forma concentrada, no Estado de São Paulo e sul do Triângulo Mineiro. Esses fatos também acarretam em grande vulnerabilidade fitossanitária, com ameaças crescentes de pragas e doenças, levando à redução da produtividade e ao acréscimo dos custos de implantação e condução dos pomares. A Clorose Variegada dos Citros (CVC) ou amarelinho, causada pela bactéria Xylella fastidiosa, é um exemplo destas ameaças. De elevada severidade em laranjas doces, causa redução no tamanho dos frutos, tornando-os inaptos para a comercialização in natura ou para produção de suco concentrado. Recentemente, as perdas em produção causadas pela CVC têm sido estimadas em até 10-14% da safra comercializável. Atualmente, o manejo da CVC está baseado na utilização de mudas sadias, poda de ramos afetados, e controle dos vetores. Além dessas medidas, é importante manter os tratos culturais exigidos no pomar. Entretanto, a utilização de cultivares resistentes é estratégia imprescindível para convivência com a doença em longo prazo. Este trabalho buscou avaliar o desempenho horticultural da laranjeira Folha Murcha, da tangerineira Satsuma cv. Okitsu e da limeira ácida Tahiti sobre doze porta-enxertos. Tangerina Satsuma cv. Okitsu e lima ácida Tahiti são espécies não sintomáticas em relação à CVC e tornam-se opções com potencial de exploração em pequenas propriedades. A laranjeira Folha Murcha tem demonstrado maior tolerância à CVC comparativamente aos demais cultivares de laranja doce. Os experimentos foram conduzidos na Estação Experimental de Citricultura de Bebedouro, EECB, (SP), e instalados em 2001. Em todos os experimentos foram avaliados o desenvolvimento vegetativo, produção e qualidade dos frutos (por no mínimo três safras) em cada combinação copa/porta-enxerto. Avaliações adicionais a respeito da tolerância à CVC foram realizadas no experimento de laranjeira Folha Murcha. Nos experimentos conduzidos sem irrigação, foi avaliada a tolerância ao déficit hídrico das copas usando duas metodologias: avaliação visual subjetiva e medição direta da coloração das folhas. Na limeira ácida Tahiti, a coloração externa dos frutos de exportação foi quantificada em função de variáveis colorimétricas, definindo-se uma metodologia quantitativa alternativa ao sistema atual de classificação dos frutos, baseado apenas na apreciação visual subjetiva. A avaliação horticultural de copas de laranjeira Folha Murcha, limeira ácida Tahiti e tangerineira Satsuma cv. Okitsu em doze porta-enxertos permitiu identificar porta-enxertos alternativos ao limoeiro Cravo, sendo estes os trifoliatas Flying Dragon, FCAV e Rubidoux. / Brazilian citriculture is mainly based on one rootstock cultivar, the Cravo Rangpur lime, exploring a restricted number of scion cultivars. This situation limits the expansion of citrus production for fresh fruit export, due to the limited number of cultivars in use. Besides, near 80% of citrus orchards are located in an area of approximately 600,000 ha, in the São Paulo State and the southern region of the Minas Gerais State. These facts also impose a large phytossanitary vulnerability to citrus production, due to the increasing threats of pests and diseases outbreaks, that cause productivity decrease and higher costs for orchard establishment and management. The Citrus Variegated Chlorosis (CVC), caused by the xylem-limited bacterium, Xylella fastidiosa, is one example of such threats. This disease is extremely severe in sweet oranges, causing size reduction ad quality deterioration of fruits, that become unsuitable for fresh consume or juice processing. The losses associated to CVC have been recently estimated in 10-14% of the total comercial crop in São Paulo State. CVC management is currently based on the use of healthy nursery plants, pruning of injured branches and control of the vectors. In addition to these strategies, it is important to maintain adequate cultural practices for orchard management. Nonetheless, the utilization of resistant cultivars is an indispensable strategy to co-exist with the disease in the long term. This study was aimed to evaluate the horticultural performance of Folha Murcha sweet orange, Okitsu Satsuma mandarin and Tahiti lime grafted onto twelve rootstocks. Okitsu Satsuma mandarin and Tahiti lime are assymptomatical species in relation to CVC, with potential to be produced in small areas. The Folha Murcha sweet orange has demonstrated high tolerance to CVC, when compared with other sweet orange cultivars. The trials were conducted at the Estação Experimental de Citricultura de Bebedouro, (EECB), and were planted in 2001. Evaluations of plant growth, yield and fruit quality were carried out in all the trials, during three years. In the Folha Murcha trial, additional evaluations of CVC tolerance were conducted. In the non-irrigated trials, tree water deficit tolerance was evaluated by two methods: visual assessment of water stress and direct leaf color measurements. In Tahiti lime, peel color of export fruits was measured and a quantitative methodology was defined as an alternative for current classification criteria, which are based on the subjective visual inspection of external fruit aspect. The horticultural evaluation of Folha Murcha sweet orange, Okitsu Satsuma mandarin and Tahiti lime onto twelve rootstocks allowed to identify the trifoliate rootstocks Flying Dragon, Rubidoux and FCAV as alternatives to Cravo Limeira.

Cucurbit[8]uril (CB[8]) has attracted great interest in the cucurbit[n]uril (CB[\textit{n}]) family on account of its capability to simultaneously accommodate two guests inside its cavity, to form strong yet dynamic ternary complexes. Owing to the photo-induced \textit{trans} to \textit{cis} isomerisation property, azobenzene (Azo) derivatives have been widely employed in several host-guest systems, leading to various light-responsive materials. This thesis focuses on CB[8]-based ternary complexes, especially those involving Azo derivatives. These systems can be exploited as a platform to hierarchically fabricate supramolecular constructs, including crystalline structures and composite materials. Specifically, novel morphology-controlled (1D needle-like, 2D sheet-like) crystals have been prepared by adjusting the assembly of Azo-CB[8] complexes, which can be further developed to oriented macroscopic free-standing crystalline pillars grown from a glass surface. Next, a composite micelle-nanoparticle complex has been prepared utilising Azo-CB[8] assemblies, which demonstrates $\sim$90$\%$ efficiency in surfactant recycling. Finally, an organic CB[8]-mediated hydrogel reinforced by inorganic nanowires has been prepared. This hybrid structure shows increased stiffness due to various supramolecular interactions. Chapter 1 gives a brief introduction to CB[\textit{n}] host-guest chemistry with emphasis on CB[\textit{n}]-based crystalline structures and CB[8] ternary complexes. Recent progress of Azo-based host-guest chemistry is then reviewed. In addition, shape-controlled crystals formed \textit{via} supramolecular interactions are discussed at the end of the chapter. Chapter 2 focuses on the crystalline structure of the 1:2 homoternary complex formed between CB[8] and a methyl orange (MO) guest, which is the fastest CB[8]-based crystallisation to date. As a commonly used pH indicator, MO possesses an azobenzene moiety with both an electronically positive amino group and an anionic sulfonate group. At low pH values, formation of the homoternary complex 2MO@CB[8] occurs, serving as a 'tectonic' building block, which rapidly stacks into a herringbone arrangement. The intermolecular and intercomplex interactions inside 2MO@CB[8] crystals are discussed, whereby the CB[8] macrocycle orients the electrostatic charges on MO guests resulting in the repulsive interactions being shielded; this in turn leads to fast electrostatically-driven crystal growth. The 2MO@CB[8] system provides a promising approach for designing ultrarapid crystallisation systems derived from CB[\textit{n}] host-guest complexes. Moreover, the host-guest chemistry between CB[8] and a variety of Azo derivatives with different functional groups is discussed, demonstrating the influence of guest structures on their crystalline behaviours. Chapter 3 further explores the mechanism of 2MO@CB[8] crystallisation through a series of experimental and computational methods. Control over the crystal shape, length and growth rate can be achieved in a facile manner whilst maintaining the same (internal) unit cell. Therefore, the properties of the macro-scale crystals can be tuned at the molecular level through adjusting the assembly of 2MO@CB[8] building blocks. For example, tuning the ionic strength of the solution enables a second growth dimension, yielding 2D crystals with sheet-like and more complex morphologies. Furthermore, our understanding of oriented electrostatics provided by the homoternary tecton can then be exploited to prepare oriented macroscopic free-standing crystalline pillars grown from a glass surface at room temperature. Next, CB[8] ternary complexes have been employed as 'bridges' to link (organic) soft and (inorganic) hard materials together, resulting in composite materials. In chapter 4, a micelle-nanoparticle complex (\textbf{MNC}) structure has been assembled \textit{via} host-guest interactions between Azo-functionalised, cargo-loaded micelles and magnetic SiO$_2$ nanoparticles (NPs) functionalised with CB[8] catenanes. Owing to the photo-responsive and magnetic properties, \textbf{MNCs} can be exploited to recycle detergents (micelles) from aqueous solution. This is followed by the controlled release of the encapsulated hydrophobic molecules inside the micelle cavity. In this process, both the micelles and NPs can be recycled efficiently. The novel \textbf{MNC} structure provides a promising approach to recycle versatile drug carriers through host-guest chemistry. Chapter 5 introduces a CB[8]-based hydrogel in which inorganic nanowires (NWs) are employed to enhance the gel stiffness. The supramolecular hydrogel is comprised of methylviologen-functionalised poly(vinyl alcohol) (PVA-MV), hydroxyethyl cellulose with naphthyl moieties (HEC-Np) and CB[8]. The gel structure is effectively enhanced by the framework supporting effects of CePO$_4$ NWs and additional hydrogen bonding interactions between NWs and PVA-MV/HEC-Np polymers. The high aspect ratio NWs serve as a 'skeleton' for the network, providing extra physical crosslinks. This results in a single continuous phase hybrid supramolecular network with improved strength, showcasing a general approach to reinforce soft materials. Finally, this thesis closes with a summary and perspective chapter, concluding the present work and highlighting an insight towards future work. Utilising CB[8] ternary complexes, various supramolecular constructs can be prepared through hierarchical self-assemblies, leading to a wide variety of composite systems and functional materials in the future.

In this work an investigation to find new method of extraction of pectin from orange peel with shortening time of extraction is carried out. Pectin was extracted from dried orange peel at higher temperature by employing stainless steel reactors. In this investigation the effect of extraction conditions (temperature: 120, 150, 200°C; extraction time: 5, 15, 30 min) with sulphuric acid, on the yield and purity of citrus pectin was performed. Moreover, optimizing other methods of extraction and analyzing of pectin is tested. In addition, effect of adding special reagent such as Sodium polyphosphate to extract solvent examined. Furthermore, two different acids are used for extraction and the results are compared as described. Pectin is solubilized by HCl and H2SO4 at different concentrations, retention time and temperature. The effect of extraction conditions (pH: 1.5 to 2; temperature: 80 to 90°C; extraction time: 1 to 3 hours) with sulphuric acid, on the yield and purity of citrus pectin was carried out. The solubilized pectin is precipitated from the solution by using organic solvents such as 2-propanol and ethanol and then analysis was done with different methods such as carbozole and meta-hydroxy-diphenyl method. A same test was run for hydrochloric acid and comparison between them was done. / Uppsatsnivå: D

Orientador: Thais Borges César / Banca: Lívia Gussoni Basile / Banca: Paulo Inácio da Costa / Banca: Thábata Koester Weber / Banca: Anderson Marliere Navarro / Resumo: O presente estudo teve como objetivo avaliar o efeito do consumo regular do suco de laranja nos parâmetros nutricionais, hemodinâmicos e bioquímicos de pacientes com hepatite C crônica. Participaram do estudo 66 indivíduos adultos de ambos os sexos, divididos em quatro grupos: Grupo com tratamento medicamentoso e com suco de laranja (MedSuco), Grupo sem tratamento medicamentoso e com suco de laranja (Suco), Grupo com tratamento medicamentoso e sem suco de laranja (Med) e Grupo sem tratamento medicamentoso e sem suco de laranja (Controle). Os participantes dos grupos Suco e MedSuco receberam 500 mL/dia de suco de laranja pasteurizado durante 8 semanas. As variáveis antropométricas utilizadas foram: peso, altura, dobras cutâneas do tríceps, bíceps, subescapular e supra ilíaca e circunferência da cintura. Para a avaliação hemodinâmica foram verificadas a pressão arterial sistólica e a diastólica, e para a avaliação dietética foi utilizado o registro alimentar, durante três dias alternados. Para a avaliação bioquímica foram realizadas dosagens de colesterol total, colesterol de HDL, triglicérides, glicemia de jejum, insulina, proteína C reativa, peroxidação lipídica, capacidade antioxidante, TNF-, interleucinas 6 e 10 e carga viral. Todas as avaliações foram realizadas antes e após o consumo do suco de laranja. Os resultados mostraram que após o consumo do suco de laranja, os pacientes do grupo Suco tiveram redução significativa do LDL-C (-9,9%), pressão arterial sistólica (-4.5%), insulina (-26.5%), índice HOMA (-32.0%), peroxidação lipídica (-68.5%), proteína C reativa (-48.8%) e TNF- (-11.7%), e aumento da capacidade antioxidante (1.2%); e os pacientes do grupo MedSuco tiveram redução significativa do LDL-C (-13.7%), pressão arterial sistólica (5.1%), HDL-C (-12.2%), peroxidação lipídica (-39.1%), proteína C reativa (-74.9%), TNF- (-8.8%) e carga viral (26.5%), e aumento da capacidade... / Abstract: This study aimed to evaluate the effect of regular orange juice consumption on nutritional parameters, hemodynamics and biochemicals of patients with chronic hepatitis C. The study included thirty-six adults of both sexes, divided into four groups: Group with drug treatment and orange juice (MedJuice), Group without drug treatment and with orange juice (Juice), Group with drug treatment and no orange juice (Med) and Group without drug treatment and no orange juice (Control). The participants of groups Juice and MedJuice received 500 mL / day of pasteurized orange juice for 8 weeks. The anthropometric variables were studied: weight, height, skin folds of the triceps, biceps, subscapular and suprailiac and waist circumference. For hemodynamic evaluation were found systolic and diastolic blood pressure, and for dietary assessment was used food records for three alternate days. For the biochemical evaluation were performed in total cholesterol, HDL cholesterol, triglycerides, fasting glucose, insulin, C-reactive protein, lipid peroxidation, antioxidant capacity, TNF-α, interleukins 6 and 10 and viral load. All evaluations were performed before and after consumption of orange juice. The results showed that after the consumption of orange juice, patients of Juice group had significant reduction in LDL-C (-9.9%), systolic blood pressure (-4.5%), insulin (-26.5%), HOMA index (-32.0%), lipid peroxidation (-68.5%), C-reactive protein (-48.8%) and TNF-α (-11.7%) and increase in antioxidant capacity (1.2%); and patients of MedJuice group had significant reduction in LDL-C (-13.7%), systolic blood pressure (5.1%), HDL-C (-12.2%), lipid peroxidation (-39.1%), C-reactive protein (-74.9% ), TNF-α (-8.8%) and viral load (26.5%) and increase in antioxidant capacity (2.5%). There was no significant decrease in anthropometric variables. Consumption of orange juice increased by 334.0% to intake of vitamin C and 63.6% folate intake of participants in the ... / Doutor

Resumo: A mancha preta dos citros (MPC), causada pelo fungo Guignardia citricarpa, produz lesões nos frutos que os depreciam para o mercado interno e os restringem para a exportação. O grande período de suscetibilidade dos frutos, em adição ao fato do patógeno causar infecções latentes, dificulta o entendimento do período de incubação da doença. O objetivo do trabalho foi determinar se o período de incubação da mancha preta do citros. Inoculou-se frutos de laranjeira 'Valência' em diferentes estádios fenológicos e também foi determinado a importância das pulverizações de fungicida cúprico, da fase de queda das pétalas e, até sete meses após, no controle da mancha preta dos citros. Os resultados obtidos indicaram uma relação linear negativa entre o período de incubação da MPC e o diâmetro dos frutos inoculados. O período de incubação em frutos inoculados, ainda imaturos (2,0, 2,5 e 3,0 cm de diâmetro), foi longo, sendo de 266 dias nos de menor diâmetro menor, enquanto que em frutos já desenvolvidos (5,0 e 7,0 cm de diâmetro) o período para expressão de sintomas foi mais curto, sendo de cerca de 30 dias. A mancha preta em frutos cítricos apresenta período de incubação variável, dependente do estádio fenológico em que os frutos tornaram-se infectados. A concentração do inóculo de Guignardia citricarpa não interfere no período de incubação da doença. Em relação à incidência da MPC, mesmo com seis pulverizações de cobre a cada 28 dias, 53,3% dos frutos expressaram sintomas da doença. Quanto à severidade, não há diferença de resposta entre os tratamentos. Portanto, os dados mostraram que as infecções precoces interferem na qualidade visual do fruto, porém não na produção. Com o crescimento dos frutos, as infecções interferem negativamente na produção, levando a um aumento na queda de frutos / Abstract: The citrus (Citrus spp.) black spot, caused by Guignardia citricarpa produces lesions on the fruit that depreciate the domestic market and to restrain them for export. The great period of susceptibility of the fruits in addition to the fact that the pathogen causing latent infection of complicates the understanding of the incubation period of the disease. The aim of this study was to determine the incubation period of the citrus black spot is constant or variable, inoculating fruits of 'Valência' orange at the different phenological stages and was also given the importance of copper fungicide sprays, stage of petal drop and up to seven months in the control of citrus black spot The results indicate a negative linear relationship between the incubation period of the CBS and the diameter of the fruits inoculated. The incubation period observed in fruits inoculated still immature (2,0, 2,5 and 3,0 cm diameter) was long, smaller in diameter occurred around 266 days, and fruits already developed (5,0 and 7,0 cm diameter) for the disease expression was shorter in diameter occurred in approximately 30 days. The citrus black spot has a variable incubation period depends on the phenological stages in which fruit is inoculated. The concentration of inoculum of Guignardia citricarpa not interfere with the incubation period of the disease. Regarding the incidence of CBS, even with six copper sprays every 28 days, 53.3% of the fruit expressed disease symptoms. For severity, there is no difference in response between treatments. Therefore, the data showed that early infections influence the visual quality of the fruit, but not in production. With the growth of the fruit, infections can affect negatively the production, leading to an increase in fruit drop / Doutor

Orientador: Sarita Leonel / Banca: Sérgio Lázaro de Lima / Banca: João Domingos Rodrigues / Banca: José Eduardo Creste / Banca: José Orlando de Figueiredo / Resumo: O trabalho teve como objetivo avaliar a eficiência da utilização de adubos verdes em pomar de laranjeira 'Pêra' (Citrus sinensis (L.) Osbeck) e foi instalado na fazenda Três Irmãos, no município de Botucatu/SP. O solo da propriedade é denominado Neossolo Quartzarênico. As plantas estão enxertadas em limoeiro 'Cravo' e foram plantadas num espaçamento de sete por quatro metros, no ano de 1996, estando com sete e oito anos de idade respectivamente nos anos de 2003 e 2004, ocasião em que foi realizado o experimento. Foram empregados quatro tratamentos correspondentes aos adubos verdes avaliados, sendo eles: feijão de porco (FP) (Canavalia ensiformis DC), labe-labe (LL) (Dolichus lablab L.), feijão guandu anão (GA) (Cajanus cajan L. Millsp) e braquiária (BQ) (Brachiaria brizantha Hochst ex A. Rich. Stapf). Os adubos verdes foram semeados no mês de dezembro dos anos de 2003 e 2004, sendo ceifados e direcionados para a linha da cultura na ocasião do pleno florescimento, o que corresponde a cento e vinte dias após a semeadura dos mesmos. Adotou-se os mesmos tratos culturais indicados para a cultura, nos dois anos de experimentação. O delineamento experimental foi em blocos ao acaso com quatro tratamentos, seis repetições, duas plantas úteis para as avaliações, completamente rodeadas por plantas bordadura. As características avaliadas foram: análise química de solo nas linhas e entrelinhas da cultura, diagnose foliar, diagnóstico nutricional através do DRIS (Sistema Integrado de Diagnose e Recomendação), circunferência do tronco (cm), volume de copa (m3), índice relativo de clorofila (índice Spad), peso médio dos frutos (g) número de frutos por caixa, rendimento de suco (%), acidez total (g de ácido cítrico / 100g de polpa), sólidos solúveis totais (SS), ratio, produtividade, porcentagem de matéria seca dos adubos verdes...(Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The purpose of the experimental was to evaluate the efficiency of the use of the green manures on an orchard planted with orange (Citrus sinensis Osbeck) cv. 'Pera' and was carried out in a farm named 'Três Irmãos', situated in Botucatu/SP/Brazil. The soil is Oxic Quartzipsamments. Plants are grafted on 'Cravo' lemon tree and were planted spaced 7 x 4 m apart, in 1996, and were seven and eight years old in 2003 and 2004, respectively when the experiment was conducted. Four different treatments were applied corresponding to the three green manures: jack bean (FP) (Canavalia ensiformis DC), lab-lab (LL) (Dolichus lablab L.), dwarf guandu (GA) (Cajanus cajan L. Millsp) and brachiaria (BQ) (Brachiaria brizantha Hochst ex A. Rich. Stapf) as a control. They were sown on December, 2003 and 2004, being mowed and directed to the line plants by the occasion of the full flowering, wich happened one hundred and twenty days after sowing. The same treatments were utilized in both experimental years. The experimental design was a randomized blocks with four treatments, six replications, and two useful plants per plot. Characteristics evaluated were: chemical analysis of the soil over the planting lines and in the middle of the citrus orchard rows, chemical analysis of the leaves, nutritional diagnosis through the DRIS, trunk circumference (cm), volume of the canopy (m3), relative index of chlorophyll (SPAD index), average fruits weight (g), number of fruits per boxes, juice content (%), total acidity (g of citric acid / 100 g of pulp), total soluble solids, ratio, productivity, percentage of dry matter and the contents of macro and micronutrients in the green manures and control (BQ). The results obtained allowed the following conclusions: the nutritional diagnosis elaborated through DRIS showed...(Complete abstract click electronic access below) / Doutor

A citricultura brasileira está fundamentada predominantemente sobre apenas um cultivar porta-enxerto, o limoeiro Cravo, e explora poucos cultivares copa, de uma maneira geral. Desse modo, o setor citrícola fica limitado na abertura de suas fronteiras de exportação para frutas frescas por estar baseado em um pequeno número de cultivares. Além disso, ao redor de 80% dos plantios estão reunidos em uma região compreendida por quase 600 mil ha, de forma concentrada, no Estado de São Paulo e sul do Triângulo Mineiro. Esses fatos também acarretam em grande vulnerabilidade fitossanitária, com ameaças crescentes de pragas e doenças, levando à redução da produtividade e ao acréscimo dos custos de implantação e condução dos pomares. A Clorose Variegada dos Citros (CVC) ou amarelinho, causada pela bactéria Xylella fastidiosa, é um exemplo destas ameaças. De elevada severidade em laranjas doces, causa redução no tamanho dos frutos, tornando-os inaptos para a comercialização in natura ou para produção de suco concentrado. Recentemente, as perdas em produção causadas pela CVC têm sido estimadas em até 10-14% da safra comercializável. Atualmente, o manejo da CVC está baseado na utilização de mudas sadias, poda de ramos afetados, e controle dos vetores. Além dessas medidas, é importante manter os tratos culturais exigidos no pomar. Entretanto, a utilização de cultivares resistentes é estratégia imprescindível para convivência com a doença em longo prazo. Este trabalho buscou avaliar o desempenho horticultural da laranjeira Folha Murcha, da tangerineira Satsuma cv. Okitsu e da limeira ácida Tahiti sobre doze porta-enxertos. Tangerina Satsuma cv. Okitsu e lima ácida Tahiti são espécies não sintomáticas em relação à CVC e tornam-se opções com potencial de exploração em pequenas propriedades. A laranjeira Folha Murcha tem demonstrado maior tolerância à CVC comparativamente aos demais cultivares de laranja doce. Os experimentos foram conduzidos na Estação Experimental de Citricultura de Bebedouro, EECB, (SP), e instalados em 2001. Em todos os experimentos foram avaliados o desenvolvimento vegetativo, produção e qualidade dos frutos (por no mínimo três safras) em cada combinação copa/porta-enxerto. Avaliações adicionais a respeito da tolerância à CVC foram realizadas no experimento de laranjeira Folha Murcha. Nos experimentos conduzidos sem irrigação, foi avaliada a tolerância ao déficit hídrico das copas usando duas metodologias: avaliação visual subjetiva e medição direta da coloração das folhas. Na limeira ácida Tahiti, a coloração externa dos frutos de exportação foi quantificada em função de variáveis colorimétricas, definindo-se uma metodologia quantitativa alternativa ao sistema atual de classificação dos frutos, baseado apenas na apreciação visual subjetiva. A avaliação horticultural de copas de laranjeira Folha Murcha, limeira ácida Tahiti e tangerineira Satsuma cv. Okitsu em doze porta-enxertos permitiu identificar porta-enxertos alternativos ao limoeiro Cravo, sendo estes os trifoliatas Flying Dragon, FCAV e Rubidoux. / Brazilian citriculture is mainly based on one rootstock cultivar, the Cravo Rangpur lime, exploring a restricted number of scion cultivars. This situation limits the expansion of citrus production for fresh fruit export, due to the limited number of cultivars in use. Besides, near 80% of citrus orchards are located in an area of approximately 600,000 ha, in the São Paulo State and the southern region of the Minas Gerais State. These facts also impose a large phytossanitary vulnerability to citrus production, due to the increasing threats of pests and diseases outbreaks, that cause productivity decrease and higher costs for orchard establishment and management. The Citrus Variegated Chlorosis (CVC), caused by the xylem-limited bacterium, Xylella fastidiosa, is one example of such threats. This disease is extremely severe in sweet oranges, causing size reduction ad quality deterioration of fruits, that become unsuitable for fresh consume or juice processing. The losses associated to CVC have been recently estimated in 10-14% of the total comercial crop in São Paulo State. CVC management is currently based on the use of healthy nursery plants, pruning of injured branches and control of the vectors. In addition to these strategies, it is important to maintain adequate cultural practices for orchard management. Nonetheless, the utilization of resistant cultivars is an indispensable strategy to co-exist with the disease in the long term. This study was aimed to evaluate the horticultural performance of Folha Murcha sweet orange, Okitsu Satsuma mandarin and Tahiti lime grafted onto twelve rootstocks. Okitsu Satsuma mandarin and Tahiti lime are assymptomatical species in relation to CVC, with potential to be produced in small areas. The Folha Murcha sweet orange has demonstrated high tolerance to CVC, when compared with other sweet orange cultivars. The trials were conducted at the Estação Experimental de Citricultura de Bebedouro, (EECB), and were planted in 2001. Evaluations of plant growth, yield and fruit quality were carried out in all the trials, during three years. In the Folha Murcha trial, additional evaluations of CVC tolerance were conducted. In the non-irrigated trials, tree water deficit tolerance was evaluated by two methods: visual assessment of water stress and direct leaf color measurements. In Tahiti lime, peel color of export fruits was measured and a quantitative methodology was defined as an alternative for current classification criteria, which are based on the subjective visual inspection of external fruit aspect. The horticultural evaluation of Folha Murcha sweet orange, Okitsu Satsuma mandarin and Tahiti lime onto twelve rootstocks allowed to identify the trifoliate rootstocks Flying Dragon, Rubidoux and FCAV as alternatives to Cravo Limeira.

A citricultura brasileira é vista como atividade razoavelmente rentável no longo prazo, mas caracterizada por preocupante nível de risco. Este trabalho buscou avaliar a rentabilidade e o risco da atividade, sugerindo como forma de melhor gerenciá-los a provisão de recursos. A análise que confronta risco e retorno da atividade utiliza o método do Valor Presente Líquido - VPL. A identificação dos principais fatores (fontes de risco) responsáveis pela variabilidade do fluxo de caixa foi feita através da análise das contas do fluxo de caixa da atividade e pela estimação do modelo de auto- regressão vetorial de previsão para definir as distribuições conjuntas desses preços. As distribuições de probabilidade para a produtividade nos 19 anos de vida útil do pomar foram definidas através do teste de ajustamento de Kolmogorov-Smirnov. Utilizando o modelo do VPL foram simulados 10.000 possíveis valores para os fatores de risco que substituídos nas contas do fluxo de caixa geraram 10.000 possíveis valores de rentabilidade para a atividade. A análise do investimento no pomar de laranja mostrou que os principais fatores de risco para a citricultura são os preços recebidos pela fruta, os preços pagos pelos insumos e a produtividade das plantas. O investimento se apresentou viável economicamente para uma taxa real de desconto de 4% ao ano, com 0,01% de chances de se obter VPL<0; já, para uma taxa de desconto de 6% ao ano há 16% de chances de se obter VPL<0; e, para uma taxa de desconto de 8% ao ano, o investimento passa a ser inviável economicamente apresentando apenas 3% de chances de se obter VPL>0. Embora a rentabilidade média esperada tenha se apresentado remuneradora (6,6% ao ano), o produtor poderá necessitar de recursos para suportar os anos que apresentarem fluxo de caixa negativo. Para gerenciar os riscos do projeto, foram sugeridos dois procedimentos para o cálculo da provisão, um baseado no risco de VPL<0 e outro na distribuição das receitas líquidas acumuladas capitalizadas negativas. O valor a ser provisionado para que o produtor fique protegido contra 95% dos possíveis déficits de caixa representa 17% do montante a ser aplicado nos 3 primeiros anos do projeto. Porém, o montante a provisionar depende dos recursos que o investidor disponha-se a aplicar a mais no projeto ou qual o limite de crédito com que ele pode contar para cobrir suas deficiências de caixa. / The brazilian citrus industry has seen as a reasonably profitable business in the long term, but characterized by worrying level of risk. This study below aimed to evaluate the profitability and risk of this business, suggesting that the best to manage them is the provision of resources. Net Present Value - NPV was the method used to confront risk and return of citrus industry. Identification of the main factors (sources of risk) that may cause the variability of cash flows was made by examining accounts of cash flow activity and estimating vector autoregression forecast prediction to define joint distributions. Productivity distributions in 19-year life of orchad were adjusted by Kolmogorov-Smirvov test. Using NPV model were simulated 10,000 possible values for risk factors which replaced in accounts of the cash flow generates 10,000 possible values to business return. Orange orchard investment analysis showed that the major risk factors for citrus are: prices received by fruit, prices paid for inputs and productivity of plants. The investment has been shown economically feasible based on a discount rate of 4% per year, with chance of 0.01% to get NPV <0, 16% chance of getting NPV <0 using a discount rate of 6% per year and using a discount rate of 8% per year the investment becomes economically unfeasible, it means only 3% chance of getting NPV> 0. Although the expected average return has been shown profitable (6.6% per year), producers may need resources to support negative cash flow years. In order to manage project risks, we suggest two procedures to calculate the provision: a risk-based on NPV <0 and distribution of accumulated capitalized and negative net income values. Provision value to protect the producer against 95% of possible annual deficits represents 17% of first 3 years investment value. However, the total amount to be provisioned will depend on investors further resources available to apply in the project or based on credit limit available to cover cash deficits.

Orientador: Magali Monteiro da Silva / Banca: Alexandre Ferreira Gomes / Banca: Marina Ansolin / Resumo: Objetivo: O objetivo desse trabalho foi avaliar o perfil de compostos fenólicos do suco de laranja integral pasteurizado de marcas comerciais. Métodos: Foram reunidas informações sobre os compostos fenólicos considerando suas características químicas, bioatividade, técnica analítica utilizada para a sua determinação, e os principais compostos presentes em citros. Sucos de laranja integral pasteurizado de nove marcas comerciais foram adquiridos em Araraquara e região, SP. Foram adquiridas três embalagens de cada marca, com diferentes prazos de validade, referentes a diferentes lotes. Sucos de cada marca e lote foram liofilizados e submetidos à extração com solução de metanol:água (90:10, v/v), e os extratos foram submetidos à limpeza em cartucho SPE C18. A separação dos compostos foi realizada no sistema HPLC-DAD, empregando coluna C18 com fase móvel de água:ácido fórmico (99,9:0,1, v/v) e acetonitrila, em modo gradiente, vazão de 1 mL.min-1, temperatura de 50 ºC e volume de injeção de 20 µL. Foi também empregado o sistema UPLC-UV/MS, com escalonamento das condições para escala sub-2 µm. Os extratos foram injetados no sistema HPLC-DAD e UPLC-UV/MS para a identificação e quantificação dos compostos fenólicos. Resultados: Os principais compostos fenólicos das frutas cítricas descritos na literatura são os flavonoides, particularmente as flavanonas narirutina e hesperidina. Nesse trabalho foram identificados 60 compostos do suco de laranja, dos quais 54 são compostos fenólicos. O... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Objective: The aim of this work was to evaluate the profile of phenolic compounds of pasteurized orange juice from commercial brands. Methods: The phenolic compounds where characterized considering chemical structure, bioactivity, and techniques for analytical determination, as well as the main compounds present in citrus. Pasteurized orange juices from nine commercial brands were purchased in Araraquara and region, SP. Three packages from each brand were acquired, with different shelf life corresponding to different lots. Juices were lyophilized and extracted with methanol:water solution (90:10, v/v), and the extracts were cleaned in C18 SPE cartridges. Separation of the compounds was performed in an HPLC-DAD system, using a C18 column with mobile phase of water:formic acid (99.9:0.1, v/v) and acetonitrile in gradient mode, with a flow rate of 1 mL.min-1, temperature of 50 ºC and injection volume of 20 μL. An UPLC-UV/MS system was also used, with scaling conditions for sub-2 μm scale. The extracts were injected into the HPLC-DAD and UPLC-UV/MS system for identification and quantification of the phenolic compounds. Results: The main phenolic compounds of citrus fruits described in the literature are flavonoids, particularly the flavanones narirutin and hesperidin. In this work 60 compounds were identified in orange juice, of which 54 are phenolic compounds. The flavonoids rutin, eriocitrin, narirutin, naringin, hesperidin, naringenin, nobiletin and tangeritin, and the caffeic, p-coumaric and ferulic acids were identified in all juices. The levels of phenolic compounds varied amongst brands and lots. The results of the PCA... (Complete abstract click electronic access below) / Mestre

>Magister Scientiae - MSc / The reservoir quality of the Orange Basin, offshore South Africa is known to be immensely impaired by the presence of authigenic minerals. The collective effects of burial, bioturbation, compaction and chemical reactions between rocks, fluid and organic matter conclusively determined the quality of reservoirs within the Orange basin. The aim of this study was to provide information on the quality of reservoirs within the Orange Basin. Data used to conduct this study include wireline logs (LAS format), well completion reports and core samples from potential reservoir zones of wells K-A2, K-A3 and K-E1. To accomplish the aim, petrophysical parameters were calculated, such as porosity, permeability and water saturation. Besides, depositional environments were identified using gamma ray log and core logging techniques. Thirdly, petrographic studies were supporting techniques in understanding how various minerals and diagenetic processes play a role in reservoir characterisation. Geophysical wireline logs (Gamma ray, Resistivity, Bulk density and Caliper) allowed for the estimation of the three main reservoir properties; namely: porosity, water saturation and permeability. The porosity calculations revealed a range of 3-18% for well K-A2, 2%-13% for well K-A3 and 3%-16% for well K-E1. The permeability’s ranged from 0.08-0.1 mD and 0.001-1.30 mD for K-A3 and K-E1, respectively. Thus, the findings of the petrophysical evaluation of the wells in Interactive Petrophysics indicated that the reservoir intervals of wells K-A2, K-A3 and K-E1 are of poor to good quality. Based on the core analyses, the depositional environment is mostly shallow marine, specifically tide dominated for well K-A2, sandstone channel for well K-A3 and intertidal environment for well K-E1. These environments were confirmed by XRD, revealing glauconite as the prominent mineral.