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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Estudo da acessibilidade em um prédio da Faculdade de Direito do Recife/UFPE

SOBRINHO, Ramiro Augusto de Miranda 07 December 2016 (has links)
Submitted by Rafael Santana (rafael.silvasantana@ufpe.br) on 2017-12-22T12:07:40Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) DISSERTAÇÃO Ramiro 25 janeiro 2017.pdf: 3027670 bytes, checksum: 767c13dc68901ac299c9f8e5065e448f (MD5) / Made available in DSpace on 2017-12-22T12:07:41Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) DISSERTAÇÃO Ramiro 25 janeiro 2017.pdf: 3027670 bytes, checksum: 767c13dc68901ac299c9f8e5065e448f (MD5) Previous issue date: 2016-12-07 / O objetivo dessa dissertação é diagnosticar e propor recomendações para a acessibilidade de Pessoas com Deficiência (PcD) e Pessoas com Mobilidade Reduzida (PMR), usuárias do espaço do prédio sede da Faculdade de Direito do Recife (FDR). O foco da pesquisa foi avaliar a estrutura física, equipamentos e mobiliário, em especial àqueles localizados ao longo da Rota Acessível, implantada no ano de 2015. Na metodologia, foi desenvolvida uma pesquisa exploratória e descritiva, utilizando-se de dados documentais legais, de bibliografia sobre o tema e da estratégia do estudo de caso. Para tanto, foram efetuadas entrevistas semiestruturadas, aplicação de planilhas de acessibilidade e análise de plantas arquitetônicas do local. As entrevistas esclareceram a percepção das PcD e PMR, com o intuito de serem identificados os constrangimentos sofridos no espaço físico da FDR. As planilhas de acessibilidade tiveram o propósito de verificar a situação do espaço acessível na FDR, em conformidade com a legislação específica para as PcD, e ênfase na NBR 9050/2015. Foram comparadas as plantas arquitetônicas da rota acessível com a real situação do espaço. Realizaram-se observações diretas com registro em fotografias e gravador de voz. As informações coletadas foram analisadas qualitativamente. O exame dos dados da pesquisa demonstrou que há necessidade de alterações no ambiente para promover segurança e comodidade. Depois de caracterizados os resultados, constatou-se que a acessibilidade no prédio é parcialmente atendida, e foram identificadas as áreas que necessitam de intervenções, ajustes e melhorias. Ao final, sugere-se à administração central da FDR o desenvolvimento de ações que contemplem a acessibilidade. A dissertação buscou aprofundar a temática da acessibilidade, no âmbito da Universidade Federal de Pernambuco (UFPE), promover a sua reflexão, e assim possibilitar a autonomia universal no uso dos seus espaços. / The aim of this dissertation is to analyze and to propose recommendations for accessibility of disabled persons (PcD in Portuguese) and persons with reduced mobility (PMR in Portuguese), user of the headquarter building of Faculdade de Direito do Recife (FDR) dependencies. The research focus was evaluate physical structure, equipments and furnishings, in a special way those located throughout accessible route, established in the year 2015. The methodology developed based on an exploratory and descriptive research, handling legal document data, bibliography data and case study strategy. Therefore, it were accomplished semi-structure interviews, accessibility spreadsheets and building blueprints analysis. The interviews have brought to the light the PcD and PMR persons’perception to intent to identify the awkwardness suffered by them in the FDR dependencies. The accessibility spreadsheets have had purpose to verify the accessible space conditions in the FDR in compliance with specific legislation to PcD persons andemphasis in NBR 9050/2015. A comparison between arquitectonic blueprints of the accessible route and on the spot condition carried out. It were accomplished direct observations with register in photography and voice recorder. It was made a data collected qualitative analysis. The data exam demonstrated the necessity to carry out modifications into the environment to promote safe and wellbeing. As result of data characterizing, it realized the accessibility conditions werenot properlyattended.In addition, improvement opportunities, interventions and adjustments were found. At the end, the report suggest to central administration of FDR developing actions that include accessibility conditions. The dissertation reached deepen itself onto the accessibility thematic at the Universidade Federal de Pernambuco (UFPE) ambit, to promote pensiveness about theme, and this way enable universal autonomy inside the academy spaces.
22

Analýza silového zatížení stopkových fréz s PCD s ohledem na jejich ostření / Analysis of the force loading of the end mills with PCD considering their sharpening

Trčka, Tomáš January 2017 (has links)
Measurement of the force loading is a significant type of measurement in mechanical engineering applied to analyse important aspects in the machining process. In addition to the determination of specific material constants, it is possible to analyse, for example, various machining strategies, geometries of tools or the degree of wear of the cutting edges of cutting tools. This master’s thesis deals especially with the last two mentioned aspects which are subjects of an experiment on end mills with PCD inserts sharpened by a different technology. In the introductory part the cutting forces are theoretically analysed along with their measurement possibilities and the technology of the production of the mills including their sharpening – WEDM and EDM-G. One chapter is concerned with the milling of plastic materials, as PVC was the material used for the workpiece during the experiment. The first experimental part is devoted to the analysis of sharpened tools followed by the measurement of forces using the piezoelectric dynamometer and the subsequent analysis of the influence of the different tool geometry. The last conducted experiment monitors how the overall cutting forces of individual end mills sharpened by different technology changed over specific milling time slots.
23

Měření doby života nosičů proudu ve strukturách křemíkových solárních článků / Lifetime measurement of current carriers in silicon solar cells structures

Macháček, Martin January 2009 (has links)
This thesis deals with a lifetime measurement of current carriers in silicon solar cell structures. In the first chapter there is a description of several recombination models and their participation at a final effective lifetime value. By using these recombination models in a computer simulation it is possible to receive approximate evaluation of some important silicon solar cell structure parameters. The PC1D simulation program was used for this thesis. For the lifetime measurement of real test-wafers two methods were used: QSSPC (quasi-steady-state photoconductance) and MW-PCD (microwave photoconductance decay). There is a detail description of these methods, used measurements machines and differences between both of them in the chapter four. The main objective of the thesis is mentioned in the last chapter, which is mainly focused on a chemical passivation of silicon wafers and deals with a problem of post-passivation wafer cleaning. There are three passivation techniques mentioned: the iodine in ethanol solution, the iodine and polymer in ethanol solution and the quinhydron in methanol solution. In two cases a results, that are adequate to return the tested wafers in the manufacture process, were achieved.
24

Dissociation of Spatial Navigation and Visual Guidance Performance in Purkinje Cell Degeneration (Pcd) Mutant Mice

Goodlett, Charles R., Hamre, Kristin M., West, James R. 10 April 1992 (has links)
Spatial learning in rodents requires normal functioning of hippocampal and cortical structures. Recent data suggest that the cerebellum may also be esential. Neurological mutant mice with dysgenesis of the cerebellum provide useful models to examine the effects of abnormal cerebellar function. Mice with one such mutation, Purkinje cell degeneration (pcd), in which Purkinje cells degenerate between the third and fourth postnatal weeks, were evaluated for performance of spatial navigation learning and visual guidance learning in the Morris maze swim-escape task. Unaffected littermates and C57BL/6J mice served as controls. Separate groups of pcd and control mice were tested at 30, 50 and 110 days of age. At all ages, pcd mice had severe deficits in distal-cue (spatial) navigation, failing to decrease path lengths over training and failing to express appropriate spatial biases on probe trials. On the proximal-cue (visual guidance) task, whenever performance differences between groups did occur, they were limited to the initial trials. The ability of the pcd mice to perform the proximal-cue but not the distal-cue task indicates that the massive spatial navigation deficit was not due simply to motor dysfunction. Histological evaluations confirmed that the pcd mutation resulted in Purkinje cell loss without significant depletion of cells in the hippocampal formation. Teese data provide further evidence that the cerebellum is vital for the expression of behavior directed by spatial cognitive processes.
25

Therapieoptimierung aggressiver Non-Hodgkin-Lymphome durch modifizierte anti-CD20-Antikörper: Präklinische Evaluation von GA101 / Therapy optimization of aggressive non-Hodgkin's lymphoma: Preclinical evaluation of GA101

Schroer, Hinrich 21 November 2012 (has links)
No description available.
26

Avaliação histocitológica, histoquímica e morfofisiológica da habituação e senescência em pupunheiras mantidas in vitro / Histocytological, histochemistry and morphophysiological evaluations of habituation and senescence on peach palm maintained in vitro

Graner, Érika Mendes 05 November 2013 (has links)
A técnica de cultura de tecidos permite a propagação rápida e maciça de propágulos geneticamente semelhantes, isentos de doenças, sendo amplamente empregada para a obtenção de gemas adventícias e embriões somáticos visando principalmente, a multiplicação clonal sob ação de reguladores de crescimento. Resultados satisfatórios foram obtidos com a espécie Bactris gasipaes Kunth. por meio da regeneração direta de gemas adventícias e de embriões somáticos, no entanto, as consequências decorrentes da prolongada manutenção in vitro de espécies perenes como a pupunheira, não estão elucidadas, sendo que as pesquisas mais expressivas ocorrem com espécies anuais e restritas a órgãos específicos. Considerando que o tempo de cultivo pode promover a senescência e a habituação de determinados tecidos aos reguladores de crescimento, afetando consideravelmente o potencial morfogênico, o objetivo principal do presente trabalho foi investigar a ocorrência destes processos em folhas, raízes e bases caulinares de plântulas e microplantas com um e oito anos de cultivo, respectivamente. Para tanto, o processo de senescência foi monitorado por meio de análises histológicas, ultraestruturais e histoquímicas à detecção de substâncias ergásticas e à fragmentação do DNA, ao passo que o processo de habituação, foi monitorado por meio de análises morfofisiológicas, histológicas e histoquímicas. Os resultados pertinentes ao processo de senescência evidenciaram a ocorrência de intenso processo de morte celular programada nas células de diversos tecidos nas estruturas analisadas das microplantas, sendo que estes eventos foram escassos e limitados às bases caulinares nas plântulas. Além disso, foi observada a presença elevada de plastoglóbulos no interior dos cloroplastos e de compostos fenólicos nas estruturas foliares e radiculares das microplantas. Já, em relação aos resultados obtidos à detecção do processo de habituação nestas microplantas, quando comparados às plântulas, foram detectados problemas relacionados ao alongamento da parte aérea e do sistema radicular, bem como alterações morfológicas nas raízes e uma pronunciada redução no potencial morfogênico das células pré-procambiais em relação às plântulas. Estes resultados evidenciam que a manutenção in vitro de pupunheiras por longos períodos promoveu o envelhecimento dos propágulos em decorrência à senescência generalizada, bem como provavelmente os conduziu ao processo de habituação aos reguladores de crescimento ANA e/ou BAP, inviabilizando a propagação em grande escala desta espécie. / The tissue culture technique allows rapid and massive spread of propagules genetically similar, free from diseases, being the technique widely employed to obtain adventitious buds and somatic embryos mainly targeting the clonal multiplication under the action of growth regulators. Satisfactory results have been obtained with the specie Bactris gasipaes Kunth. through direct regeneration of adventitious buds and somatic embryos, however, the consequences from prolonged in vitro maintenance of perennial species such as peach palm are not clear, and the most significant research occur with annual species and restricted to specific organs. Whereas the cultivation time can promote senescence and habituation of certain tissues to the growth regulators, affecting considerably the morphogenic potential, the main objective of this study was to investigate the occurrence of these processes in leaves, roots and stem bases of seedlings and microplants with one and eight years of cultivation, respectively. Thus, the senescence process was monitored by histological, ultrastructural and histochemical detection of ergastic substances and DNA fragmentation, whereas the habituation process was monitored by analyses histologic, histochemic and morpho-physiological. The relevant results from the senescence process showed the occurrence of an intensive process of programmed cell death in cells of various tissues of the analised microplants structures, and these events were rare and limited to the stem bases in the seedlings. Furthermore, it was observed the high presence of plastoglobules inside chloroplast and phenolic compounds in the leaf and root structure of microplants. Already, the results obtained in relation to the detection of the habituation process in these microplants, when compared to the seedlings, were detected problems related to the elongation of shoots and roots, as well as morphological changes in roots and a pronounced reduction in the morphogenic potential of pre procambial cells compared to seedlings. These results demonstrate that the in vitro maintenance of peach palm for long periods promoted the aging of seedlings due to senescence widespread and probably led to the habituation process, to the growth regulators NAA and/or BAP, difficulting the propagation on a large scale of this species.
27

Valuation - The issue of illiquidity : A qualitative retake on illiquidity discounts in the context of private company valuation on the Swedish market

Fredlund, Viktor, Tollerup, Andreas January 2015 (has links)
A private company lacks a direct observable market value and several situations may require a practitioner to compute the value of a private company. Since most of the valuation methods in use are based on data derived from the public stock markets certain adjustments may be appropriate when valuing a private company. Marketability and liquidity is said to be one of the more observable differences between a public and a private company. This implies that the shares in a private company have a lack of marketability and liquidity in comparison to the shares in a public company, which practitioners may have to adjust for. Several quantitative studies are conducted on the subject in order reassure price differences between public and private companies, namely a private company discount (PCD). Furthermore, several quantitative studies strive to establish a general and standardized cost for lack of marketability (liquidity) expressed as the illiquidity discount or the discount for lack of marketability (DLOM). These studies have different perceptions and use different hypothesis to identify illiquidity, which in turn will lead to a large span of different discounts. Essentially, earlier research examines assets marketability and liquidity with the assumption of them being equal in all other aspects. Professional practitioners constantly seek guidance in these studies to justify their estimated and applied illiquidity discount/DLOM when performing a valuation on a privately held company. Furthermore, we have also observed survey-studies adopting a more qualitative method in order to appreciate the level of discounts applied in a valuation by professional practitioners. Consequently, this sea of studies provides the practitioner with a discount that ranges from 5% to 60% to take a stand on. The impossibility to determine the most adequate theory contributes to the inconsistency of how this issue is handled in reality by market participants and courts. In our study we first provide the reader with a rigorous literature study, which describes earlier research on the subject of illiquidity discount/DLOM. We conclude that research has gone one step too far when conducting all of these quantitative studies. This is why we conduct our own empirical data through semi-structured in-depth interviews with professional valuation experts on the Swedish market. This makes our approach a retake on the issue in order to generate suggestions to further studies. What we find is that all of the independent consultants, primarily, does not apply a discount when valuing a majority interest due to the paradigm on the Swedish market. In contrast, the private equity fund manager, which only acquires majority interest, can use this type of discounts in their dependent valuation of majority interests. However, when valuing a minority interest the independent valuation consultants use quantitative empirical studies to derive a starting point of the discount. The level of the discount is then estimated upon the purpose of the valuation and firm-specific variables, which all of the participant’s states to be the most important ones when estimating a illiquidity discount/DLOM. Based on these results we argue that one should be very careful when taking guidelines from quantitative empirical studies. Our interpretation is that the level of illiquidity/DLOM applicable depends on the level of attractiveness, which in turn has a bearing on all firm-specific variables. When it comes to applying the appropriate discount all of the participants argue in favor for a discount-on-value and not as some research suggest; a risk premium added to the discount rate. We also generate adequate suggestions to further studies based on these interviews. Since courts and in particular the Swedish tax-court is inconsistent when approving or rejecting illiquidity discounts/DLOM we suggest legal actions on the issue. Furthermore we suggest a survey-like study in order to catch consensus take on how to estimate the level of discount. In fact, this can be done every year in a similar way as PwC’s market risk premium study is conducted.
28

Innovative cutting materials for finish shoulder milling Ti-6A1-4V aero-engine alloys

Oosthuizen, G. A. 03 1900 (has links)
Thesis (MScEng (Industrial Engineering))--University of Stellenbosch, 2009. / The titanium alloys have found wide application in the aerospace, biomedical and automotive industries. Soaring fuel prices and environmental concerns are the fundamental drivers that intensify the demand situation for titanium. From a machining viewpoint, one of the challenges companies face, is achieving high material removal rates while maintaining the form and function of the part. The ultimate aim for a machining business remains to make parts quickly. Conventional cutting speeds range from 30 to 100 m/min in the machining of Ti-6Al-4V. Milling this alloy faster however is challenging. Although titanium is becoming a material of choice, many of the same qualities that enhance titanium‟s appeal for most applications also contribute to its being one of the most difficult materials to machine. The author explored the potential for Polycrystalline diamond (PCD) inserts in high speed milling of Ti-6Al-4V, by trying to understand the fundamental causes of tool failure. The objective was to achieve an order of magnitude increase in tool life, while machining at high speed, simply by reducing some of the failure mechanisms through different cutting strategies. Tool wear is described as a thermo-mechanical high-cycle fatigue phenomenon. The capability of a higher material removal per tool life is achieved in the case of PCD inserts compared to Tungsten carbide (WC). The average surface roughness produced was relatively low. The collected chips were also analyzed. The work demonstrated progress over the performance reported in current literature. The work confirms that there is a region where a sufficiently high temperature in the cutting zone may contribute to extended tool life, provided that the tool material can withstand these extreme conditions.
29

Percepção das pessoas com deficiência sobre a Atenção à Saúde e sua contribuição para a educação médica

Lins, Valéria Cristina Montenegro Batista 13 April 2018 (has links)
Submitted by Automação e Estatística (sst@bczm.ufrn.br) on 2018-06-05T23:39:47Z No. of bitstreams: 1 ValeriaCristinaMontenegroBatistaLins_DISSERT.pdf: 1615522 bytes, checksum: fec706c1392cd10cc0a4945ec2ad59fd (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-06-13T22:51:30Z (GMT) No. of bitstreams: 1 ValeriaCristinaMontenegroBatistaLins_DISSERT.pdf: 1615522 bytes, checksum: fec706c1392cd10cc0a4945ec2ad59fd (MD5) / Made available in DSpace on 2018-06-13T22:51:30Z (GMT). No. of bitstreams: 1 ValeriaCristinaMontenegroBatistaLins_DISSERT.pdf: 1615522 bytes, checksum: fec706c1392cd10cc0a4945ec2ad59fd (MD5) Previous issue date: 2018-04-13 / A Política Nacional de Saúde da Pessoa com Deficiência orienta ações de promoção da saúde, prevenção de incapacidades, melhoria dos mecanismos de informação, capacitação profissional, e organização/funcionamento dos serviços de atenção à saúde. No entanto, as pessoas com deficiência não se sentem assistidas em suas necessidades quando procuram tais serviços. Adicionalmente, a educação médica, em geral, não tem contemplado em seus conteúdos curriculares esse grupo de pessoas, contribuindo assim, para o não reconhecimento das especificidades relacionadas aos vários tipos de deficiência e de aspectos relevantes para o cuidado integral. Esse estudo objetiva compreender a percepção das pessoas com deficiência sobre o atendimento médico recebido nos serviços de saúde, de modo a contribuir para uma formação profissional que atenda às necessidades dessa população específica. O caminho teórico-metodológico percorrido foi a fenomenologia, utilizando a abordagem qualitativa. As técnicas utilizadas para a coleta dos dados foram grupo focal e entrevistas semiestruturadas, de onde emergiram três unidades temáticas: Necessidades das pessoas com deficiência; Serviços de Saúde e Atenção integral à saúde das pessoas com deficiência. Os resultados dessa pesquisa evidenciaram que as pessoas com deficiência, quando recorrem aos serviços de saúde buscando atendimento médico, muitas vezes, se deparam com barreiras presentes nas instituições, tanto no que se refere ao aspecto atitudinal e de comunicação, quanto à acessibilidade e à falta de equipamentos e materiais apropriados ao seu atendimento. Observou-se também a necessidade de sensibilização e capacitação dos profissionais médicos para melhor atendimento e acolhimento às pessoas com deficiência, no sentido de contribuir para que elas se sintam incluídas socialmente e respeitadas na sua condição. Reitera-se a importância do papel da atenção primária no cuidado integral à saúde dessas pessoas, o que se constitui um desafio e provoca uma reflexão sobre a eficácia das práticas atuais e sobre as possibilidades de melhorias futuras. Conclui-se que é necessário que as escolas médicas se comprometam com a temática e possibilitem um ensino voltado para as necessidades em saúde dessas pessoas. / The National Policy on the Health of Persons with Disabilities guides actions of health promotion, disability prevention, improvement of information mechanisms, professional training, organization and operation of health care services. However, persons with disabilities do not feel assisted in their needs when seeking such services. In addition, medical education, in general, has not included in its curriculum content this group of persons, thus contributing to the non-recognition of the specificities related to the various types of disability and aspects relevant to integral care. This study aims to understand the perception of persons with disabilities about the medical care received in health services, so as to contribute to professional training that meets the needs of this specific population. The theoretical-methodological path covered was phenomenology, using the qualitative approach. The techniques used to collect data were focal group and semi-structured interviews, from which emerged three thematic units: Needs of persons with disabilities; Health Services and Comprehensive health care for persons with disabilities. The results of this research showed that persons with disabilities, when they use health services seeking medical attention, often face barriers in the institutions, both at the level of attitudinal and communication, accessibility, to the lack of equipment and materials appropriate to their service. There was also a need for awareness and training of medical professionals to better care and accommodate persons with disabilities, in order to make them feel socially included and respected in their condition. The importance of the role of primary care in the integral health care of these persons is reiterated, and it is a challenge, reflecting on the effectiveness of current practices and the possibilities for future improvements. It is concluded that it is necessary for medical schools to commit themselves to the theme and to provide education aimed at the health needs of these persons.
30

Avaliação histocitológica, histoquímica e morfofisiológica da habituação e senescência em pupunheiras mantidas in vitro / Histocytological, histochemistry and morphophysiological evaluations of habituation and senescence on peach palm maintained in vitro

Érika Mendes Graner 05 November 2013 (has links)
A técnica de cultura de tecidos permite a propagação rápida e maciça de propágulos geneticamente semelhantes, isentos de doenças, sendo amplamente empregada para a obtenção de gemas adventícias e embriões somáticos visando principalmente, a multiplicação clonal sob ação de reguladores de crescimento. Resultados satisfatórios foram obtidos com a espécie Bactris gasipaes Kunth. por meio da regeneração direta de gemas adventícias e de embriões somáticos, no entanto, as consequências decorrentes da prolongada manutenção in vitro de espécies perenes como a pupunheira, não estão elucidadas, sendo que as pesquisas mais expressivas ocorrem com espécies anuais e restritas a órgãos específicos. Considerando que o tempo de cultivo pode promover a senescência e a habituação de determinados tecidos aos reguladores de crescimento, afetando consideravelmente o potencial morfogênico, o objetivo principal do presente trabalho foi investigar a ocorrência destes processos em folhas, raízes e bases caulinares de plântulas e microplantas com um e oito anos de cultivo, respectivamente. Para tanto, o processo de senescência foi monitorado por meio de análises histológicas, ultraestruturais e histoquímicas à detecção de substâncias ergásticas e à fragmentação do DNA, ao passo que o processo de habituação, foi monitorado por meio de análises morfofisiológicas, histológicas e histoquímicas. Os resultados pertinentes ao processo de senescência evidenciaram a ocorrência de intenso processo de morte celular programada nas células de diversos tecidos nas estruturas analisadas das microplantas, sendo que estes eventos foram escassos e limitados às bases caulinares nas plântulas. Além disso, foi observada a presença elevada de plastoglóbulos no interior dos cloroplastos e de compostos fenólicos nas estruturas foliares e radiculares das microplantas. Já, em relação aos resultados obtidos à detecção do processo de habituação nestas microplantas, quando comparados às plântulas, foram detectados problemas relacionados ao alongamento da parte aérea e do sistema radicular, bem como alterações morfológicas nas raízes e uma pronunciada redução no potencial morfogênico das células pré-procambiais em relação às plântulas. Estes resultados evidenciam que a manutenção in vitro de pupunheiras por longos períodos promoveu o envelhecimento dos propágulos em decorrência à senescência generalizada, bem como provavelmente os conduziu ao processo de habituação aos reguladores de crescimento ANA e/ou BAP, inviabilizando a propagação em grande escala desta espécie. / The tissue culture technique allows rapid and massive spread of propagules genetically similar, free from diseases, being the technique widely employed to obtain adventitious buds and somatic embryos mainly targeting the clonal multiplication under the action of growth regulators. Satisfactory results have been obtained with the specie Bactris gasipaes Kunth. through direct regeneration of adventitious buds and somatic embryos, however, the consequences from prolonged in vitro maintenance of perennial species such as peach palm are not clear, and the most significant research occur with annual species and restricted to specific organs. Whereas the cultivation time can promote senescence and habituation of certain tissues to the growth regulators, affecting considerably the morphogenic potential, the main objective of this study was to investigate the occurrence of these processes in leaves, roots and stem bases of seedlings and microplants with one and eight years of cultivation, respectively. Thus, the senescence process was monitored by histological, ultrastructural and histochemical detection of ergastic substances and DNA fragmentation, whereas the habituation process was monitored by analyses histologic, histochemic and morpho-physiological. The relevant results from the senescence process showed the occurrence of an intensive process of programmed cell death in cells of various tissues of the analised microplants structures, and these events were rare and limited to the stem bases in the seedlings. Furthermore, it was observed the high presence of plastoglobules inside chloroplast and phenolic compounds in the leaf and root structure of microplants. Already, the results obtained in relation to the detection of the habituation process in these microplants, when compared to the seedlings, were detected problems related to the elongation of shoots and roots, as well as morphological changes in roots and a pronounced reduction in the morphogenic potential of pre procambial cells compared to seedlings. These results demonstrate that the in vitro maintenance of peach palm for long periods promoted the aging of seedlings due to senescence widespread and probably led to the habituation process, to the growth regulators NAA and/or BAP, difficulting the propagation on a large scale of this species.

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