Este trabalho teve como objetivo avaliar um programa de suplementação de luz no desenvolvimento, desempenho e comportamento de bezerros em aleitamento e o uso da câmera termográfica como método para avaliação de variáveis fisiológicas por método não invasivo. O experimento foi realizado na Agência Paulista de Tecnologia dos Agronegócios - APTA, em Ribeirão Preto, SP. Foram utilizados 20 bezerros Jersey e mestiços da raça, divididos em dois tratamentos, com e sem programa de suplementação de luz artificial. O programa de suplementação de luz artificial consistiu em 6 h de luz artificial (das 00:00 as 6:00 h), completando 18 horas de luz por dia. Foram avaliadas as características fisiológicas dos animais: frequência respiratória (FR), temperatura da superfície do pelame (TSP), temperatura auricular (TA), temperatura caudal (TC) e temperatura retal (TR). A ingestão de ração e água também foi estudada. Avaliou-se também o desenvolvimento dos animais através dos ganhos médios diários de peso (GPMD), de altura, de comprimento e de perímetro torácico (PT). Foi estudado o comportamento social e ingestivo dos animais. E por fim, através de cinco coletas de dados em dias de alta entalpia, onde os dados foram registrados em quatro horários (18:00, 00:00, 06:00 e 12:00 h). Foram registradas as seguintes variáveis fisiológicas: TSP e TC, TA, e TR (método convencional). As fotos termográficas foram registradas com uma câmera termográfica, nos mesmos dias e horários de registros das variáveis fisiológicas pelo método convencional. As variáveis fisiológicas não apresentaram diferença significativa entre os tratamentos (P>0,0001), porém apresentaram diferença entre os horários de coleta, sendo que as maiores temperaturas foram encontradas na coleta das 12 h. O consumo de água e ração no experimento não teve variação significativa (P>0.01). Nos dias de alta entalpia a ingestão de água variou somente para o período de 31 a 75 dias de idade dos animais. O desenvolvimento dos animais mostrou diferença quando foram submetidos ao programa de suplementação de luz. O GPMD e o PT dos animais foi maior (P<0,001) para o tratamento sem luz, a altura foi influenciada (P=0,008) pela idade dos animais e o comprimento variou de acordo com o tratamento (P=0,006) e a idade (P=0,001). O programa de suplementação de luz não mostra diferenças nas variáveis fisiológicas e no consumo de alimento de bezerros em aleitamento, e o desenvolvimento dos animais é prejudicado nesta fase. O comportamento social dos animais não sofreu efeito significativo (P>0,05) do tratamento de luz, porém os períodos do dia dividido de seis em seis horas, influenciou a comportamento dos animais. As atividades de ócio e dormindo são as mais frequentes na criação de bezerros em aleitamento. O comportamento ingestivo é alterado pela presença de luz e pelo período do dia, sendo que os animais mostram maior consumo de água e ração quando não são submetidos a luz artificial. Os valores obtidos pelos dois métodos de colheita de dados foram correlacionados. Contudo, é possível relacionar fotos termográficas de bezerros em aleitamento com o método convencional de registro de temperaturas corporais. Para este trabalho, utilizou-se a temperatura auricular, na qual foi apresentada uma regressão para obter as demais temperaturas corporais do animal. / This study aimed to evaluate a light supplementation program on the development, performance and behavior of suckling calves and the use of thermographic camera as an evaluation method of physiological variables by a non-invasive method. The experiment was conducted in Sao Paulo Agency of Agribusiness Technology - APTA, in Ribeirao Preto, SP, Brazil. Were used 20 Jersey and crossbred calves, divided in two treatments, with and without artificial light supplementation. The light supplementation program consisted on 6 hours of artificial light (from 00:00 to 6:00 h), completing 18 hours of light per day. Were evaluated the physiological characteristics of the animals: respiratory frequency (RF), coat surface temperature (CST), ear temperature (ET), tail temperature (TT) e rectal temperature (RT). The ingestion of food and water was also considered. Was also evaluated the development of the animals through the daily average weight gain (BG), height, length and thoracic girth (TG). It was also evaluated the social and ingestive behavior of the animals. The experiment consisted of collecting data on five days of high enthalpy, where the data were recorded at four times (18, 00, 06 and 12 hours). It recorded the following physiological variables: coat surface temperature (CST) and flow temperature (FT) with infrared thermometer, ear temperature (ET) with digital ear thermometer, and rectal temperature (RT) with digital thermometer (conventional method). The thermography images were recorded with a thermography camera, on the same days and hours of record of physiological variables by the conventional method. Physiological variables showed no signicant difference between treatments (P>0,0001), but differ between the collection times, and the highest temperatures were found in the collection of 12 h. The water and food intake in the experimente did not change significantly (P>0.01). In the days of high water intake varied only for the period from 31 to 75 days old animals. The development of the animals showed a difference when they were submitted to the program of supplemental ligth. The ABW and TG of the animals was higher (P<0,001) for the treatment without light, and was also influenced (P=0,008)bye the age and length of the animals varied according to treatment (P=0,006) and age (P=0,001). The programo f supplementary light shows no diferences in physiological parameters, feed intake of suckling calves, and development of animals is impaired at this stage. The social behavior of animals was not affected (P>0,05) of the light treatment but the periods of the divided every six hours, influenced the behavior of animals. The leisure and sleeping activities are the most frequente in the creation of suckling calves. Feeding behavior is altered by the presence of light and time of day, and the animals show higher consumption of water and food when they are not subjected to artificial light. The values obtained by two methods of data collection were correlated. It is possible to relate thermography pictures of suckling calves on the conventional method if recording body temperatures. For this work we use the ear temperature, which was presented in a regression to obtain the rest of the animal body temperatures.

Durante o início da primavera, na região oeste do Estado de São Paulo, fêmeas Suffolk (n=91) foram tratadas com 1 (1M) ou 2 implantes (2M) subcutâneos de 18 mg de melatonina (Mélovine), acompanhadas por um lote controle (C), e submetidas ao efeito macho por rufiões, 42 dias após a colocação do implante. Após nove dias, os rufiões foram substituídos pelo reprodutor, tratado com 01 implante, com o qual as fêmeas permaneceram durante 51 dias, em sistema de monta a campo. O diagnóstico de gestação foi efetuado por ultra-sonografia e apresentou 36,7%, 43,3% e 38,7% de animais gestantes no primeiro exame, e ao segundo, 36,7%, 46,7 e 48,4% para os grupos 1M, 2M e C, respectivamente. Não houve diferença significativa entre o intervalo efeito macho/cios férteis (20,8 &plusmn; 10,26; 25,0 &plusmn; 12,75 e 27,0 &plusmn; 9,68 dias), encarneiramento/acasalamentos férteis (11,8 &plusmn; 10,26; 16,0 &plusmn; 12,75 e 18,0 &plusmn; 9,68 dias), taxa de fertilidade (43,3%; 53,3%; 51,6%), taxa de parição (36,7%; 53,3% e 48,4%), prolificidade (1,36; 1,50 e 1,27) entre os grupos 1M, 2M e C, respectivamente. A ocorrência de um efeito de facilitação social, provavelmente permitiu que fêmeas não tratadas (C) voltassem a ciclar. O tratamento com melatonina foi capaz de adiantar a estação reprodutiva, permitindo acasalamentos de primavera/verão e proporcionando parições de outono/inverno. / Ninety one Suffolk ewes were randomly allotted to none (control group), single (group 1M) or two (group 2M) melatonin slow releasing subcutaneous implants (18 mg, Mélovine) during spring season in the west region of São Paulo. Fourty two days after the implantation ewes were submitted to ram effect trough vasectomized teasers exposition and substituted by a ram treated with one melatonin implant following 51 days of mating period. Pregnancy diagnosis was performed by real-time ultrasound showing 38.7, 36.7 and 43.3% of pregnancy for groups C, 1M and 2M at the first examination, and 48.4, 36.7 and 46.7% of pregnancy respectively at the second examination. It was observed no significant difference between ram effect / fertile oestrus (27.0 &plusmn; 9.68, 20.8 &plusmn; 10.26, 25.0 &plusmn; 12.75 days), mating / pregnancy (18.0 &plusmn; 9.68, 11.8 &plusmn; 10.26, 16.0 &plusmn; 12.75 days), fertility rate (51.6%, 43.3%, 53.3%), parturition (48.4%, 36.7%, 53.3%), prolificity (1.27, 1.36, 1.50) for groups C, 1M and 2M respectively. The occurrence of social facilitation effect robably permitted the untreated group (control group) returned to cycle. Melatonin treatment advanced the reproductive season permitting spring / summer mating and autumn / winter parturitions.

Largemouth bass (Micropterus salmoides) is one of the species of fish that don´t have a significant position among the species of fish breeded in Czech Republic. However the trend of building aquaculture recirculation systems with warm water comes to the fore. Breeding in these conditions opens up the possibility of introducing new kinds of thermophilic fish species. Largemouth bass definitely belongs to this group. The general factors of successful breeding of largemouth bass are the temperature and purity of the water, the suitable-composed diet and the correct stocking density. This work studies another factor that not only participates in the success of the breeding, but also has influence on the energy expenses of the aquaculture system. It is the effect of photoperiod during breeding. Five experimental groups with three repetitions were created according to the time of lighting (8, 12, 16, 20 and 24 hours of light a day). The experiment lasted 16 weeks. The average water temperature was 24,6 °C. The initial stocking density for all tanks was 20.6 kg . m-3. Fish had an initial average weight of 32.6 +- 2.1 grams. The feed ration was set up at 1% of biomass per day. As a diet were used a floating dry pellets Skretting R-2 Europa 15 F of 2 mm. The feed contained 55 % of protein and 16 % of fat. Fish were fed manually by hand. All groups showed high survival during the experiment (99.4 - 100 %). The best results were achieved by a group of 8 hours with the highest feed conversion (FCR = 0.94 +- 0.06) and the highest growth rate (SWGR = 0.6 +- 0.04 % . day-1). The average individual fish weight of the 8 hour group at the end of the experiment was 64.07 +- 2.82 g. On the other hand, group of 12 hours was the worst with the lowest average individual weight (W= 57.51 +- 1.51 g), growth rate (SWGR = 0.49 +- 0.04 % . day-1) and the lowest feed conversion (FCR = 1.16 +- 0.08). These two groups were significantly different in most production indicators. The pervisceral fat index grew with biomass. The longer the photoperiod, the higher the plasma and ammonia content of lactate. In contrast, glucose content decreased. A longer photoperiod also influenced the higher production of antioxidants.

A expressão do gene Kiss1 é essencial para o controle neuroendócrino da reprodução, pois esses neurônios regulam a atividade dos neurônios que secretam o hormônio liberador de gonadotrofinas, e portanto, o ciclo ovulatório. Em roedores sazonais, o aumento da síntese de melatonina durante o inverno é responsável pela inibição das funções reprodutivas, fato que é acompanhado pela diminuição da expressão desse gene. Devido à importância dos neurônios Kiss1 no desenvolvimento do sistema reprodutivo, o presente estudo investigou a participação da melatonina na regulação da expressão do gene Kiss1 e sua influência na reprodução, em um modelo animal não sazonal. Concluimos que condições de dias curtos (que possivelmente aumentam o período de circulação de melatonina) alteram parâmetros de amadurecimento sexual em fêmeas e, assim como o tratamento com melatonina, afetam o tamanho das gônadas de camundongos machos, mas que esses efeitos não alteram o número de neurônios que expressam o gene Kiss1 e não são suficientes para inibir a reprodução das fêmeas. / The expression of Kiss 1 gene is essential for the neuroendocrine control of reproduction, as these neurons regulate the activity of neurons that secrete gonadotropin-releasing hormone, and therefore the ovulatory cycle. In seasonal rodents, the increase in melatonin synthesis during winter is responsible for the inhibition of reproductive functions, a fact that is accompanied by decreased expression of this gene. Because of the importance of Kiss1 neurons in the development of the reproductive system, the present study investigated the involvement of melatonin in the regulation of expression of Kiss1 gene and its influence on reproduction in a non-seasonal animal. We conclude that short-day conditions (which possibly increase the period of melatonin circulation) alter sexual maturation parameters in females, and as well as melatonin treatment, affect the size of the gonads of male mice, but these effects do not change the number neurons expressing the Kiss1 gene and are not sufficient to inhibit the reproduction of female mice.

Despite teleosts being the largest and most diverse group of vertebrates, fish models currently used to study photoperiodic effects on fish physiology have been limited to a few species, most of which are temperate seasonal breeders. The overall aim of this work was to expand our knowledge on circadian biology and environmental physiological effects in Nile tilapia (Oreochromis niloticus niloticus), a continuous breeding species of tropical-subtropical origin. The circadian light axis of Nile tilapia is described with regards to melatonin production. Circadian melatonin profiles of fish under 12L:12D photoperiods were observed to be low at day and high at night, suggesting melatonin to be an entraining signal as observed in all other vertebrates. When constant light (LL) was used, such day and night fluctuations where abolished. However when fish where exposed to constant darkness (DD) a strong robust endogenous melatonin rhythm was found, suggesting the presence of circadian oscillators in this species. Importantly, this endogenous rhythm was observed to be maintained for at least three weeks under darkness and proved to be circadian in nature. Moreover, although the melatonin system was able to produce day and night melatonin rhythms when exposed to a different (6L:6D) photocycle, the oscillator appeared to not be entrainable to such a short photo cycle when exposed to DD, as melatonin levels remained high. When comparing the circadian organization of different teleost species including Nile tilapia, preliminary studies showed at least three divergent circadian light organizations in teleosts. Nile tilapia was characterised by a pineal gland far less sensitive than in other fish species as demonstrated through in vitro studies. Furthermore, pineal melatonin production was clearly dependent on the light perceived by the eyes as ophthalmectomy resulted in basal plasma melatonin levels during the dark period. These findings are the first to be reported in a teleost and could be comparable to the circadian light organization of higher vertebrates such as mammals. The onset of puberty of Nile tilapia was studied with regards to the newly discovered Kiss1/GPR54 system. Such a system has recently been discovered in mammals and found to be the primary switch of the brain-pituitary-gonadal (BPG) axis. The results of this study not only suggest a link between the Kiss1/GPR54 system and the onset of III puberty in this tropical batch spawning teleost, that would be a highly conserved feature across vertebrates, but also that the transcriptional mechanisms regulating GPR54 expression could be directly or indirectly influenced by light. Finally, a study was conducted on the effects of different intensities of continuous light (LL) on the growth and sexual development of Nile tilapia up to first maturation. The results showed a significant growth response of fish in all LL treatments compared to control fish. Importantly, this confirmed that LL enhances growth in this species and suggests that it is the light regime more than the intensity which is having an effect. This work thus provides important basic knowledge of the light entrainment pathway and circadian melatonin rhythms in Nile tilapia. Of special importance is the discovery of a strong endogenous melatonin oscillator and a novel circadian organization in fish which would seem to be homologous to that observed in higher vertebrates. Moreover, this work provides evidence that the newly discovered Kiss1/GPR54 system has a similar role in fish as has been found in mammals and that such a system could be directly or indirectly regulated by light. If so, Nile tilapia and other fish species could become important models in the chronobiology and reproduction fields. Finally, this work not only increases our basic and applied knowledge of this species, but also broadens our understanding of the circadian light axis in teleosts and its mediatory effects on reproduction.

Η εκμετάλλευση της ενέργειας των φυτών αποτελεί σημαντικό ελπιδοφόρο τομέα εναλλακτικών μορφών ενέργειας. Η αξία των ενεργειακών φυτών γίνεται ακόμα μεγαλύτερη όταν αυτά παράγονται με βιολογικό κι όχι με το συμβατικό τρόπο (χημικά πρόσθετα). Η παρούσα εργασία μελετά τον τρόπο ανάπτυξης/αύξησης της ποικιλίας Keller του γλυκού σόργου (Sorghum bicolor Moench) υπό τη συμβατική και βιολογική μέθοδο καλλιέργειας. Συγκεκριμένα, α) το βαθμό διαφοροποίησης των αγροβιολογικών και αυξητικών χαρακτηριστικών (ύψος φυτού, αριθμός φύλλων, αριθμός κόμβων,) υπό το συμβατικό και βιολογικό τρόπο καλλιέργειας, και β) την επίδραση της θερμοκρασίας και της φωτοπεριόδου στη γεωμετρία (μήκος, πλάτος, φυλλική επιφάνεια) των φύλλων του γλυκού σόργου. Για το σκοπό αυτό, εγκαταστάθηκε καλλιέργεια γλυκού σόργου σε αγρόκτημα του Πανεπιστημίου Πατρών κατά τις καλλιεργητικές περιόδους 2003, 2004 και 2005. Βρέθηκε ότι ο ρυθμός μεταβολής στο χρόνο και οι μέγιστες τιμές των παραμέτρων του ύψους, του αριθμού φύλλων, του αριθμού κόμβων, του μήκους φύλλου, του πλάτους φύλλου και της φυλλικής επιφάνειας του γλυκού σόργου που καλλιεργείται με βιολογικό τρόπο δεν διαφέρει σημαντικά έναντι του συμβατικού. Επίσης, ο συνδυασμός φωτοπεριόδου και θερμοκρασίας (εκφραζόμενος υπό του φωτοθερμοχρόνου) φαίνεται να είναι καθοριστικός στο δυνητικό αριθμό φύλλων, ενώ η θερμοκρασία (θερμοχρόνος) φαίνεται να είναι καθοριστική στις γεωμετρικές παραμέτρους του φύλλου και εντέλει στη φωτοσυνθετική επιφάνεια και φωτοσυνθετική δυνατότητα των φυτών. Γενικότερα, η βιολογική καλλιέργεια του γλυκού σόργου μπορεί να προταθεί ανεπιφύλακτα έναντι της συμβατικής με σημαντικά περιβαλλοντικά οφέλη από τη μη χρήση χημικών λιπασμάτων και άλλων χημικών επιπρόσθετων. / The exploitation of the energy potential of the plants is a dynamic aspect of the alternative agriculture. The value of energy plants is highly increased when they are the crop product of biological and not of conventional (chemical inputs) mode of cultivation. The present research investigates the developmental/growth pattern of sweet sorghum [Sorghum bicolor (L) Moench cv. Keller] under biological and conventional mode of cultivation; specifically the a) differentiation of agrobiological and growth characteristics (plant height, number of leaves, number of nodes), and b) the impact of temperature and photoperiod in geometry (length, width, leaf area) of the leaves of sweet sorghum. For these purposes, a cultivation of sweet sorghum was established in the farm of Patra’s University in 2003, 2004, 2005. It was found that the seasonal changes and the peak values of plant’s height, number of leaves, number of nodes, leaf length, leaf width, leaf area of sorghum plants biologically cultivated did not significantly differ from the plants conventionally cultivated. Also, the combined effect of photoperiod and temperature (in terms of photothermal time) determines the potential number of leaves, while the effect of temperature per se (in terms of thermal time) determines the leaf shape parameters, and in a final stage, the photosynthetic area and effectiveness of the plants. Generally, the biological cultivation of sweet sorghum is un-doubtfully suggested, in contrast to the conventional, with the additional environmental benefits of not using chemical fertilization and chemical inputs.

Flowering in Arabidopsis thalina is controlled by multiple pathways and is repressed by cold. To understand how A. thalina molecularly responds to cold stress temperatures, we subjected seedlings to different temperatures of cold stress for four days. Specifically, we conducted quantitative PCR of CONSTANS (CO) and the F-Box protein FKF1 to observe specifically how cold temperatures might affect the flowering time of a plant. We found a 16°C cold stress temperature increased both CO and FKF1 transcription when compared to a 24°C control. The increased expression of both CO and FKF1 may serve as a priming pathway that enables plants to be ready to flower at the precise moment when spring arrives.

Sexual maturation during commercial culture of Atlantic cod (Gadus morhua L.) represents a significant production bottleneck restricting the profitability of the industry. Such problems in other species have traditionally been addressed by artificial manipulation of photoperiod cycles, however little research exists in this field in cod. This thesis therefore investigates the interactions between artificial photoperiod manipulation, sexual maturation and somatic growth in this species. In the first experiment, populations of Atlantic cod (hatched, spring 1999) were maintained on either a simulated natural photoperiod (SNP) or continuous illumination (LL) from approximately 15 months post hatch (MPH) (July 2000) in an enclosed tank system. Growth performance was recorded monthly along with observations of reproductive activity over the subsequent 2 years (up to July 2002). At both 2 and 3 years of age the entire population raised under SNP matured and spawned, during which time mean weight reduced by 13% and 24% respectively. No spawning individuals were recorded at 2 years of age in the LL population and only 18% were observed to spawn at 3. However, observations of both changes in gonadal morphology (observed via ultrasound scanning) and a suppression in growth rate at 2 years of age in the LL population alluded to a maturation “dummy run” regulated by an endogenous clock. Despite this phenomenon, the LL treatment realised a 39% and 43% improvement in wet weight following 1 and 2 years of exposure to LL respectively. When the diel cycle of plasma melatonin was compared between the treatments in February 2001 (23MPH) the SNP population displayed an A-profile diel rhythm ranging between 20 and 50 pg/ml while the LL treatment did not display any rhythm. In the second experiment of this work, two populations of cod (hatched, spring 2001) were reared in commercial open cage systems, one of which experienced continuous additional artificial illumination between July 2002 (15MPH) and October 2003 (30MPH) provided by four, 400W submerged lighting units. Growth and maturation were assessed in both populations throughout. In March 2003 (24MPH) it was apparent that spawning individuals were present in both the SNP and LL populations though a significantly lower number of spawning individuals in the LL treatment suggested that the peak in spawning activity was delayed by about 1 to 2 months. With both populations apparently maturing at 2 years of age, there was no significant difference in weight between the populations at the end of the trial. In comparison to experiments I and IV of this work, these results would suggest that in comparison to salmonids for example, Atlantic cod appear to have a heightened sensitivity to light allowing individuals to differentiate the ambient photoperiod signal from the application of continuous artificial light. In the third experiment, 6 populations of approximately 20 tagged individuals (hatched spring 1999) were maintained, from December 2000 to July 2002, under either SNP, LL or one of four, out of season “square wave” photoperiod regimes (repeating cycles with a 12 month period, consisting of a 6 month window of LL followed by six months of short day lengths [SD, 7L:17D] which had been staggered to start over a six month period). Each individual was monitored monthly for maturation status. Out of season “square wave” photoperiods were demonstrated to successfully entrain maturation and hence significantly alter the spawning profiles in these populations. Application of LL from December 2000 failed to inhibit maturation in the spring of 2001 and, in fact, advanced the spawning season by 1 month while those that experienced SD from the same date showed significant extension of the subsequent spawning season. Interestingly, the males maintained on LL throughout the experiment matured both in the spring of 2001 and one year later in the spring of 2002 while females under the same treatment only matured and spawned in 2001. In the fourth experiment, a total of 830 tagged individuals were raised either under SNP or one of 7 photoperiod treatments, consisting of 5 groups transferred from SNP to LL at 3 monthly intervals between 6 and 18 MPH where they remained and a further two groups maintained on LL from 6 to 15MPH and 6 to 21 MPH respectively before being returned to SNP. Both the gonadic and somatic axes were monitored at the physiological and endocrinological level at three monthly intervals from 6 to 27 MPH. The results demonstrated that it is the falling autumnal photoperiod signal after the summer solstice, more specifically after October, that is responsible for recruiting individuals to enter the sexual maturation cycle. Furthermore, in all treatments where this signal was masked i.e. those which experienced LL starting at or prior to 15MPH, except for some restricted spermatogenic activity in the males testis observed at 27MPH, there was no significant reproductive activity and growth was improved by up to 60% at 27 MPH. While providing evidence for direct photic stimulation of somatic growth, the growth results were also correlated with the measurement of plasma IGF-I and demonstrated its potential as a tool to assess growth rates in the species. Plasma melatonin measured at 15MPH, as in experiment I, was suppressed in all populations which were under LL photoperiods. By identifying the photoperiod “window of opportunity” which recruits individuals into the sexual maturation cycle, this work was able to conclude that the application of LL from the summer solstice prior to maturation is the most efficient photoperiod strategy to be adopted by the aquaculture industry to realise maximum growth potential from their cultured stocks.

In 2007, U.S. Congress created the "Energy Independence and Security Act" with primary goals focused on increasing the knowledge in production of renewable fuels, increasing the percentages of renewable fuels in the transportation sector and decreasing the emissions of greenhouse gases from fossil fuel sources. To achieve these goals, many species have been pointed as sources of feedstock for the biofuel industry. Photoperiod sensitive (PS) biomass sorghum for the lignocellusosic based conversion is one. In this study, three main objectives were addressed regarding the relative performance for biomass yield and biomass composition of PS biomass sorghum. First, genetic and environmental variation effects on the biomass yield and biomass composition, and usefulness of pre-classification of genotypes by biomass lignin content were evaluated. On the set of genotypes and locations tested, the environmental effect had the largest influence on the biomass composition, yield and its components. Although smaller, the genetic variation effect was significant for most of the traits, some traits had significant genotype by environment GXE interaction. The pre-classification of genotypes according to lignin content proved to be an efficient system of separating genotypes as groups, but failed to be efficient in separating on the entries bases. Assessment of growth patterns for biomass yield and composition, characterized photoperiod sensitive sorghum as capable of producing a harvestable crop as soon as 4 months, but variations in the concentration of constituents and moisture percentage, pointed to a harvest window that can be extended up to the 7th month after planting. Genetic variation was observed in this trail for most agronomic and composition traits, but a strong environmental effect was also observed. Lastly, the influence of three diverse cytoplasm male sterility (CMS) systems in biomass sorghum hybrids was assessed. The presence of A1, A2 or A3 CMS in the hybrids tested in this study had no influence on the biomass yield performance or in the biomass composition. Therefore, any of the CMS systems can be used in the production of biomass sorghum hybrid seed. Also, in this trial the environmental effects were significant and strong for most traits evaluated.

Four trials were conducted to investigate factors controlling the seasonal onset of reproductive activity in red deer hinds. Firstly (Chapter 4), the role of photorefractoriness to long daily photoperiods in the initiation of the seasonal reproductive activity in breeding red deer hinds was examined. Red deer hinds (n=10) were prematurely exposed to a long daily photoperiod of 15.3 h from 22 July to 8 November 1986 i.e. winter-spring (EPW), or maintained under natural photoperiods (NP). Six hinds experienced the natural changes in daily photoperiod until mid-summer but were exposed to a 15.5 h of light each day from 30 January to 30 April 1987, i.e. summer-autumn (EPS), whilst hinds in the other groups experienced naturally decreasing daily photoperiods. On 5 occasions (July 1986, January, February, March and April 1987), blood samples were collected from 4 NP and 4 EPW hinds every 20 minutes for 4 h to monitor secretion of luteinising hormone and half hourly for another 4 h following an i.v. injection of 2 µg GnRH to measure pituitary responsiveness. In January, March and April 1987 EPS hinds were also intensively sampled for 4 h. Plasma progesterone concentrations and mean date of calving indicated that the onset of breeding activity was not affected by light treatment in the EPW hinds but was delayed by 3 weeks in the EPS hinds. In contrast, supplementary lighting caused a premature elevation of plasma prolactin concentrations and advanced pelage moulting in EPW hinds only. Plasma LH secretion patterns indicated that LH pulse frequency and mean LH concentrations were greater during the breeding season than during pregnancy or, seasonal or postpartum, anoestrus. The reduction in LH secretion was partially explained by a diminished pituitary responsiveness to GnRH. Daily plasma melatonin secretion patterns indicated that the duration of the nocturnal increase in melatonin concentrations was responsive to changes in photoperiod and provided a suitable endocrine signal for measuring day length. The results suggest that, unlike the sheep and prepubertal red deer hind, the onset of seasonal breeding activity in breeding red deer hinds is not affected by long daily photoperiods in spring but is delayed if the autumnal decrease in daily photoperiod is delayed. Therefore neither the development of photorefractoriness nor the spring increase in daily photoperiods initiated the transition from seasonal anoestrus to reproductive activity in the breeding hind. However, long daily photoperiods may have entrained the annual cycle of pelage shedding and prolactin secretion. It is possible that the neuroendocrine pathway by which photoperiodic signals entrain the seasonal cycle of reproduction is separate from those which regulate other seasonal events in the breeding red deer hind. Secondly (Chapter 5), in a study of seasonality of reproduction, 4 pubertal hinds were monitored for live weight and plasma LH and progesterone concentrations from December 1987 to October 1988 (i.e. 12-22 months of age). In addition the pattern of LH secretion was also studied in 4 ovariectomised pubertal hinds implanted s.c. with controlled release implants containing 12 mg oestradiol-17β between 4 March and 25 May and between 15 June and 19 September, 1988. On several occasions (15 December, 29 February, 15 March, 24 April, 14 June, 29 June, 18 September (all hinds) and 3 October (ovariectomised hinds only) blood samples were collected every 20 minutes for 4 h to monitor secretion of luteinising hormone and following an i.v. injection of 2 µg GnRH to measure pituitary responsiveness. Plasma progesterone profiles indicated that 4-6 ovarian cycles, lasting about 19 d each, occurred in each intact hind. Regular ovarian cycles commenced in late April (26 April ± 3.4 d, mean ± s.e.m.) and ceased 3 months later in July (21 July ± 7.2 d). The number of LH pulses in the intact hinds was higher in June (1-2 pulses/4 h) than in the non-breeding season (< 1 pulse/4 h) probably due to a seasonal increase in GnRH secretion. It appears that the seasonal increase in LH pulsatility and onset of reproductive activity in the entire hinds were temporally related to a reduction in the sensitivity of LH secretion to the negative feedback effects of oestradiol in ovariectomised pubertal hinds. In the third trial (Chapter 6), 20 male and 20 female red deer calves were immunised at birth against a melatonin conjugate or injected with adjuvant only (controls). Booster injections were given on 5 occasions over the next 2 years. Stags which produced significant melatonin binding activity in response to immunisation, were heavier than the controls between 7 and 11 months of age and at 16, 20, 30 and 34 months of age. A similar but smaller effect on live weight was seen in the immunised hinds. Immunisation against melatonin did not affect the calving date of the hinds or antler development and time of casting of antlers in the stags. These results indicated that disruption of the photoperiodic signal by immunisation against melatonin may have prevented the entrainment of annual rhythms in feed intake and growth but without affecting the seasonal cycles in antler growth and ovarian activity. Finally in a series of 3 trials (Chapter 7), anoestrous red deer hinds were induced to ovulate with the GnRH analogue, buserelin. Hinds were pre-treated with intravaginal devices containing 0.6 g progesterone (CIDR-Type S) for 14 days prior to CIDR withdrawal on 4 March. In Year 1, 15 hinds were treated with 1 CIDR each and 8 hinds were injected i.m. with 4 µg buserelin (a GnRH analogue) at CIDR removal followed by 2nd injection of 10 µg 48 h later. In Year 2, 16 hinds were treated with 2 CIDRs each and 8 hinds were injected Lm. with 4, 3, 2, 2 and 10 µg buserelin at -48, -24, 0, 24 and 48 h respectively from CIDR withdrawal. In Year 3, 15 hinds were treated with 2 CIDRs and 11 hinds (Groups Band BO) injected with buserelin identical to the protocol in Year 2. At CIDR withdrawal 6 of these buserelin treated hinds (Group BO) and 4 other progesterone-primed hinds (Group 0) were injected with 500 µg oestradiol benzoate. In addition, during the breeding season, 4 hinds (Group C) were treated with double CIDRs only for 14 d from 1 April. When oestrous behaviour and the pattern of plasma LH secretion were monitored in Year 3, oestrous behaviour was less noticeable and delayed in Group B hinds and peak LH levels were lower and increased later relative to Group 0, BO and C hinds. Two weeks after CIDR withdrawal, 6 buserelin-treated hinds in both Year 1 and Year 2, and 3/5 B, 2/4 0, 3/6 BO and 4/4 C group hinds in Year 3 had a single corpus luteum present. Plasma progesterone concentrations were elevated for about 12 d in most hinds with a corpus luteum in Year 2 and in most B, BO and C Group hinds in Year 3. However, progesterone secretion was low in several Year 1 and all Group 0 hinds in Year 3 indicating that the induced corpora lutea in these hinds were functionally subnormal. There was no evidence that any of the buserelin-induced ovulations resulted in pregnancy, probably because the induced ovulations were not accompanied by normal hormonal and behavioural patterns. The experiments described in this thesis have contributed to our understanding of the regulation of seasonal breeding in red deer hinds. This knowledge may eventually enable reproductive activity to be effectively manipulated, thereby improving the efficiency of deer production systems.