This work is concerned with development of module for communication with intelligent instalation into houses system. The aim was to familiarize with the products of RF Control of  ELKO EP, s.r.o., USB bus and wireless communication components for the 868 MHz band. And afterwards, in cooperation with the ELKO EP company, to design an electrical scheme and printed circuit board motive of this module. For cooperation with the module and for the system components control an aplication was programmed. The application name is RF-AP USB.

This diploma thesis is divided into two main parts. The first section is dedicated to the history and development of the laser technology. The second part describes all individual types of laser technology which are used in the industry nowadays. The next section follows with the research of various laser devices which serve mainly as a cutting tool. This knowledge gathered in the previous part was used to create the next part including the own conception of the machine. The second half of this diploma thesis deals with a research of software available at the market today. Eventually, after summarizing the characteristics of each software, the new concept and implementation of own software are made. In the final section, there are mentioned the possible extension and available upgrades. The reader should be able to create his/her own conception of the laser device and software after reading and understanding this paper.

Ultrasound imaging is widely used in medical examination. However, the interpretation of images is not trivial and requires much experience. In this thesis, various techniques for enhancement of visual quality of ultrasound images are presented. Several basic and advanced methods that may simplify the visual diagnosis are described. Finally, an interactive application is designed and implemented for simple usage of presented methods.

This master's thesis deals with design and developement of simple dictation system. It explains methods used for speech recognition and describes existing systems. Design of the system is focused primarily to create graphic user interface with large emphasis on user friendliness.

This thesis explores the possibilities of abstraction of graphical user interface and automation of its design and development process at runtime. Emphasis is placed on possibility of remote control over computer network and accordingly on delegation of the interface creation on the human operated remote machine. The thesis analyzes contemporary approaches to and methods of interface generation. As a goal of the thesis a system for automatic user interface generation for remote applications was designed and implemented. The generation is based on specified data model.

京都大学 / 新制・課程博士 / 博士(医学) / 甲第24485号 / 医博第4927号 / 新制||医||1063(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 江藤 浩之, 教授 湊谷 謙司, 教授 大鶴 繁 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DGAM

Two major projects involving the use of solution processed blended semiconductors for organic field effect transistors (OFET) were explored. The first incorporated unsorted single walled carbon nanotubes (SWCNTs) into a diketopyrrolopyrrole-quarterthiophene (DPP-QT) semiconductor to enhance the mobility of the OFET. 2 wt % SWCNT was found to be the optimal blend ratio, nearly doubling the device mobility (0.6 to 0.98 cm^2/V·s). Beyond this ratio, the metallic content of the SWCNT’s dropped the on/off ratio below acceptable levels. When source drain metals who’s work function poorly matched that of the DPP-QT semiconductors highest occupied molecular orbital (HOMO) were used, the SWCNT could dramatically reduce the charge injection ratio with best results achieved for Al, dropping the contact resistance from 10^5 to 45 MΩ. The second project explored the addition of small molecule additives into a UV-sensitive semiconductor 2,7-dipentyl[1]benzothieno[3,2-b][1] benzothiophene (C5-BTBT) mixed with a polymethyl methacrylate (PMMA) polymer binder. We generated a C5-BTBT based phototransistor sensitive to UV-A light. The HOMO and lowest unoccupied molecular orbital (LUMO) of C5-BTBT and the various additives were measured and discovered to play a critical role in how the device operates. We discovered if an additive has a LUMO lower in energy than C5-BTBT, it can act as a charge trap for a photogenerated electron. Electron deficient additives were found to retain a trapped electron for an extended period of time, allowing the device to remain in a high current state for an extended period of time (>1 hour). This provides an opportunity for the device to be used as an optical memory system or photoswitch. The best system could detect UV-A with a Pill > 10^5 and a photoresponsivity of 40 A/W at a Pinc of 0.0427 mW/cm^2. / Thesis / Doctor of Philosophy (PhD) / An emerging field of electronics is the use of organic materials that can be solution processed, to reduce manufacturing costs and make new and interesting products. Here we used unsorted carbon nanotubes blended into the semiconductor layer of a transistor, providing a bridge for the energy mismatch between the electrodes and the semiconductor. This allowed us the freedom to choose different metals to act as our electrodes when making electronic devices. Additionally through the correct choice of semiconductor, we added device functionality, making it responsive to UV-A light. This produced a device that could act as a UV-A sensor, logic switch or memory device. These devices are air stable and solution processable, a necessity if they are to be used in real world applications.

<p dir="ltr"><b>Background</b></p><p dir="ltr">Heart failure (HF) is a chronic clinical syndrome characterized by symptoms including breathlessness, fatigue, swelling of the ankles, and signs such as edema pulmonary crackles etc. During HF, pathogenic mechanisms including hemodynamic overload, ventricular remodeling, aberrant calcium handling, excessive neurohormonal stimulation contribute to the worsening and progression of the condition. Ventricular arrhythmias are the common cause of sudden cardiac death (SCD) in HF patients.</p><p dir="ltr">Hyperactivation of the sympathetic nervous system (SNS), a characteristic of HF, causes an increase in circulating catecholamines which becomes detrimental to-adrenergic receptors (-AR) leading to signaling dysfunction, and decrease in contractility and the ionotropic reserve. Expression of calcium/calmodulin-dependent protein kinase II (CaMKII), a downstream effector of-AR and a key regulator of calcium homeostasis, has been shown to be enhanced in HF. CaMKII-mediated mechanisms have been demonstrated to contribute to cardiac remodeling, arrhythmias by pathological regulation of ion channels, and contractile dysfunction.</p><p dir="ltr">The human ether-a-go-go related gene (hERG) encodes the pore-forming subunit of the voltage-gated potassium channel that conduct the rapid component of the delayed rectifier potassium current, <i>I</i>_Kr. The gating kinetics of <i>I</i>_Krmakes it a crucial determinant of the duration of the plateau phase of atrial and ventricular action potential (AP). Reduced <i>I</i>_Kr density due to loss-of-function mutations or pharmacological blockage of hERG channels precipitate arrhythmias. Downregulation of <i>I</i>_Kr density and protein have been reported in HF. Recent studies suggest that microRNAs (miRNAs) are involved in pathological downregulation of hERG.</p><p dir="ltr">miRNA are small non-coding RNAs of approximately 22 nucleotides in length that function as gene expression regulatory elements by repression translation. Aberrant miRNA expression has associated with cancer, cardiovascular, autoimmune, and inflammatory disorders.</p><p dir="ltr"><b>Objective</b></p><p dir="ltr">The overarching objective of this study is to investigate the mechanisms of CaMKII-mediated regulation of hERG function, including assessment of an interplay with miR-362-3p during sustained β-AR stimulation. In Specific Aim 1, the effect of CaMKII activation through sustained β-AR stimulation on hERG function and miR-362-3p expression will be assessed. The mechanism of miR-362-3p upregulation will be evaluated in Specific Aim 2, and in Specific Aim 3, the interactome of miR-362-3p and binding sites will be characterized and predicted, respectively.</p><p dir="ltr"><b>Methods</b></p><p dir="ltr">Whole-cell, voltage clamp electrophysiology experiments were performed in HEK 293 cells stably expressing hERG (hERG-HEK) and both hERG and wild-type CaMKIIδ<br>(hERG/CaMKII-HEK) following treatment with isoproterenol for 48 hours, and after transfection with miR-362-3p. The effect of CaMKII activation on miR-362-3p was assessed using real-time quantitative polymerase chain reaction (RT-qPCR). Total RNA was isolated 48 hours after isoproterenol treatment and the TaqMan assay was used to reverse transcribe and analyze miR-362-3p expression. Cells were transfected with cJun siRNA and precursor miR-362-3p to assess the role of cJun miR-362-3p upregulation during sustained β-AR stimulation with isoproterenol. The interactome of miR-362-3p was assessed in both cell lines using enhanced crosslinking immunoprecipitation (eCLIP) assay. miR-362-3p binding sites were predicted using RNAStructure Duplexfold after identification of miR-362-3p chimeric molecules from eCLIP experiment. Interaction analysis was performed using GeneMania in Cytoscape to identify genes that were potentially downregulated by miR-362-3p and been reported to interact with hERG.</p><p dir="ltr"><b>Results</b></p><p dir="ltr">In Specific Aim 1, the effect of sustained β-AR stimulation on hERG currents and endogenous miR-362-3p was assessed in hERG-HEK and hERG/CaMKII-HEK cells. Using whole-cell voltage clamp electrophysiology, we demonstrated that 48 hours treatment with 100 nM isoproterenol reduced hERG currents in hERG/CaMKII-HEK cells (p = 0.032) but had no effect on the voltage dependence of activation (p = 0.61) relative to control vehicle. Isoproterenol treatment for 48 hours, however, had no effect on hERG currents (p = 0.58) and the voltage dependence of activation (p = 0.99) in hERG-HEK cells. The effect of sustained isoproterenol treatment on miR-362-3p was also assessed using RT-qPCR. In hERG/CaMKII cells, 48 hours isoproterenol treatment increased miR-362-3p expression (2.3 folds; p = 0.038) relative to control vehicle. hERG/CaMKII-HEK cells were also treated with 500 nM KN-93 or its inactive analogue, KN-92, in an attempt to reverse CaMKII effect on miR-362-3p expression. Treatment with KN-93 decreased miR-362-3p expression (0.5-fold; p = 0.002) relative KN-92 treatment. Isoproterenol treatment had no effect on miR-362-3p expression in hERG-HEK cells (p = 0.38).</p><p dir="ltr">The regulatory mechanism of miR-362-3p expression was evaluated in Specific Aim 2. The role of an activator protein-1 (AP-1)-like sequence located at 98 base pairs upstream of miR-362-3p transcription start site was probed using siRNA inhibition of cJun, a central protein of the AP-1 complex, and deletion of the site sequence. The effect of exogenous miR-362-3p on hERG currents were first assessed. Precursor miR-362-3p decreased hERG currents (p = 0.003) compared to control plasmid. The effect of CaMKII overexpression was also assessed on exogenous miR-363-3p expression. Isoproterenol treatment in hERG/CaMKII-HEK cells transfected with precursor miR-362-3p increased mature miR-362-3p expression (0.029) compared to control vehicle treatment. Inhibition of cJun inhibition with cJun-specific siRNA decreased mature miR-362-3p expression (0.5-fold; p = 0.027) compared to scramble siRNA in hERG-HEK cells. In hERG-HEK cells transfected with mutated precursor miR-362-3p (AP-1-like site deleted), cJun inhibition with siRNA had no effect on miR-362-3p expression (p = 0.40).</p><p dir="ltr">The focus of Specific Aim 3 was to characterize the interactome of miR-362-3p as well as predict the miRNA response element (MRE) of its target mRNAs using enhanced crosslinking immunoprecipitation. A network analysis was also performed to identify miR-362-3p targets that have been reported to interact with hERG. Approximately 23% of miR-362-3p mRNA targets from the eCLIP assay have also been catalogued in miRNA database, TargetScanHuman, as miR-362-3p targets. miR-362-3p chimeric molecules with 853 unique targets, of which 75 were identified to interact with hERG through the network analysis. Four unique chimeric molecules between miR-362-3p and hERG mRNA were identified, but the interactions were non-canonical (located in the coding sequence of hERG and outside the seed region of miR-362-3p). Thirty five of the 75 miR-362-3p targets that were identified to interact had a chimeric read ≥ 3, a cutoff number indicating non-random chimeric formation. Using RNAStructure DuplexFold, miR-362-3p was predicted to form canonical binding with 12 of 35 mRNA targets. HSPA4, a heat shock protein involved in the maturation and trafficking of hERG, was identified in a canonical interaction (8-mer) with miR-362-3p.</p><p dir="ltr"><b>Conclusion</b>:</p><p dir="ltr">Sustained β-AR stimulation increases miR-362-3p expression and decreases hERG currents in CaMKII overexpressing cells. cJun mediates miR-362-3p upregulation by interacting with an AP-1-like sequence upstream of miR-362-3p transcription start site. Pathological regulation of <i>I</i>_Kr by CaMKII mediated by miR-362-3p during sustained-AR may contribute to increased risk of arrhythmias in states of increase catecholaminergic activity, such as HF.</p>

The purpose of this study is to examine whether the implementation of quantitative easing (QE) and quantitative tightening (QT) in the United States has detracted from the integrity of the country’s macroeconomic environment. In other words, does QE impact macroeconomic stability? Then, evaluate implications and externalities of stability as they relate to sustainability efforts. QE and QT are relatively new phenomena, understanding their effects and implications for the greater economy is a worthwhile endeavor, not least because QE is a current practice of so many central banks internationally. This study has two parallel investigations; first, a time series analysis conducted with a VAR model investigating the relationship of QE/QT usage by the Federal Reserve (Fed) on the macroeconomic stability of the United States. The data used in this study includes 242 monthly observations spanning January 2003 - February 2023. The second, is an OLS regression analysis evaluating whether macroeconomic stability is potentially correlated with sustainability efforts. For this study, 23 annual observations spanning 1995 – 2017 were used. Due in part to the general unavailability of genuine progress indicator (GPI) data. Based on the analysis conducted using a VAR model at lag t-4, QE has a positive relationship with Producer Price Index (PPI) and Federal Funds Rate (FFR). This is in accord with previous empirical literature on the subject. However, the second path of discovery failed to yield significant results with regard to the link between macroeconomic stability and sustainability efforts. Mention of this study’s limitations as well as avenues for future research can be found in the conclusion of this study.

Die Umsetzung einer effektiven Klimapolitik ermöglicht die Verringerung von Treibhausgasemissionen und damit die Eindämmung des Klimawandels. Eine solche Politik hat jedoch negative Auswirkungen auf die direkt oder indirekt in der fossilen Industrie tätigen Akteure, wenn deren Vermögensgegenstände wertlos werden (auch „Asset Stranding“ genannt). Diese Arbeit leistet einen Beitrag zum Verständnis der Wechselwirkung zwischen Klimapolitik und Asset Stranding: Sie untersucht angebotsseitige Politikmaßnahmen in der fossilen Brennstoffindustrie, das Ausmaß und die Verteilung von Asset Stranding auf Ebene der Anlagenbesitzer im Energiesektor und die Wechselwirkungen zwischen Asset Stranding, Erwartungen über Klimapolitik und systemischen Finanzkrisen. Darüber hinaus gibt die Arbeit einen Überblick über die Literatur im Bereich Klimaökonomie zu Asset Stranding und zu entgangenen Gewinnen der Produzenten fossiler Brennstoffe aufgrund von Klimapolitik. Die Arbeit besteht aus fünf Artikeln (Kapitel 2-6), die von einer allgemeinen Einleitung und einer Schlussfolgerung umschlossen werden. / The implementation of effective climate policies facilitates reducing greenhouse gas emissions and thereby mitigating climate change. Such policies, however, have adverse effects on stakeholders directly or indirectly engaged in the fossil industry if they find the value of their assets "stranded". This thesis contributes to our understanding of the interaction between climate policies and asset stranding: It studies supply-side policies in the upstream fossil fuel extraction industry, the extent and distribution of stranded assets at the asset owner level in the power sector, and the interaction of asset stranding, expectations of climate policies, and financial systemic crises. Further, the thesis surveys the climate economics literature on stranded assets and fossil fuel producers' lost profits due to climate policies. The thesis is a compilation of five articles (Chapters 2-6) encased by a general introduction and a conclusion.