Submitted by Fernanda Ciolfi (fernanda.ciolfi@puc-campinas.edu.br) on 2016-08-11T14:09:31Z No. of bitstreams: 1 OSVALDO PISSOLATO JR.pdf: 2291678 bytes, checksum: e7217ede7cd8cccdee8affcb06011624 (MD5) / Made available in DSpace on 2016-08-11T14:09:31Z (GMT). No. of bitstreams: 1 OSVALDO PISSOLATO JR.pdf: 2291678 bytes, checksum: e7217ede7cd8cccdee8affcb06011624 (MD5) Previous issue date: 2016-06-06 / Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP / The large disposal of construction waste, due to the renovations and demolitions as well as the waste generated by the low quality of labor, makes the construction industry one of the major polluters of the environment. In recent decades, comes to forcing organized gathering of rubble and its recycling through the enforcement of environmental laws, so that the construction industry there have disciplining towards self-manage their disposal. Studies have been intensifying to rethink the reuse of construction waste, through the recycling process, to develop standards for production, evaluation and use of recycled aggregates in order to increase their consumption, making it economically feasible to produce. This research assumes that the fine aggregate derived from the crushing of construction and demolition waste can replace the natural fine aggregate in the production of mortars for coatings. However, the issue that was faced was to what extent it would be possible to replace these materials so that the result in practice was technically and economically feasible, thereby increasing the chance of use of these recycled aggregates. In order to analyze the influence of fine aggregates derived from civil construction waste (CCW), replacing fine aggregate in the production of hydraulic and mixed mortars for wall coverings and ceilings, we used the experimental methodology to verify the influence of fines content of recycled aggregate in the mortar properties in fresh and hardened state. The prepared mortars were also classified according to NBR 13281: 2005 and evaluated for adhesion, cracking and permeability after application on masonry of concrete blocks. Therefore, we chose to study the replacement of natural aggregate by the CCW in rates of 30%, 60% and 100%, in proportions of admixture and mixed hydraulic mortar, whose purpose would be to apply as monolayer coating mortar. As analysis and discussion of the results could be seen that, in tests, both plastic and hardened state, the mortar produced with recycled aggregate, the three mixing ratios in the study had a satisfactory performance in relation to admixture reference. However, the results were not satisfactory in applicability tests, since its performance was below the minimum rate set by the standard. The results, however, suggest referral to further studies in order to continue rethinking the paradigm of industrial production so that it is founded on a model of sustainable development in favor of environmental preservation. / O grande descarte de rejeitos da constru??o civil, em fun??o das reformas e demoli??es como tamb?m do desperd?cio gerado pela baixa qualidade da m?o de obra, faz da ind?stria da constru??o civil uma das grandes poluidoras do meio ambiente. Nas ?ltimas d?cadas, vem-se obrigando o recolhimento organizado dos entulhos e sua reciclagem por meio da imposi??o das leis ambientais, de modo que a constru??o civil, paulatinamente, tem-se disciplinando no sentido de autogerir os seus descartes. Estudos v?m se intensificando com o objetivo de se repensar o reaproveitamento dos res?duos da constru??o civil, por meio do processo de reciclagem, no sentido de desenvolver normas para produ??o, avalia??o e utiliza??o dos agregados reciclados, de modo a aumentar seu consumo, tornando economicamente vi?vel a sua produ??o. Esta pesquisa parte do pressuposto de que o agregado mi?do proveniente da britagem de res?duo de constru??o e demoli??o pode substituir o agregado mi?do de origem natural na produ??o de argamassas para revestimentos. Todavia, a quest?o com a qual se deparava era at? que ponto seria poss?vel fazer a substitui??o destes materiais de modo que o resultado na pr?tica fosse vi?vel t?cnica e economicamente, aumentando assim, a chance de emprego desses agregados reciclados. Com o objetivo de analisar as influ?ncias dos agregados mi?dos oriundos dos res?duos de constru??o civil (RCC), em substitui??o ao agregado mi?do natural na produ??o de argamassas hidr?ulica e mista para revestimentos de paredes e tetos, empregou-se a metodologia experimental para verificar a influencia do teor de finos do agregado reciclado nas propriedades das argamassas no estado fresco e endurecido. As argamassas preparadas tamb?m foram classificadas conforme a NBR 13281:2005 e avaliadas quanto a ader?ncia, fissura??o e permeabilidade ap?s aplica??o em alvenaria de bloco. Para tanto, optou-se por estudar a substitui??o do agregado natural pelo de RCC nas propor??es de 30%, 60% e 100%, em propor??es de mistura de argamassa mista e hidr?ulica, cuja finalidade seria a aplica??o como embo?o paulista. Conforme an?lise e discuss?o dos resultados p?de-se constatar que, nos ensaios, tanto no estado pl?stico quanto no estado endurecido, as argamassas produzidas com reciclado, nos tr?s tra?os em estudo, tiveram um desempenho satisfat?rio, em rela??o ao tra?o refer?ncia. Entretanto, os resultados n?o foram satisfat?rios nos ensaios de aplicabilidade, visto que sua performance ficou abaixo do ?ndice m?nimo estabelecido pela norma. Os resultados, todavia, sugerem encaminhamento para novos estudos no sentido de continuar repensando o paradigma da produ??o industrial para que este seja alicer?ado num modelo de desenvolvimento sustent?vel favor?vel ? preserva??o ambiental.

Submitted by SBI Biblioteca Digital (sbi.bibliotecadigital@puc-campinas.edu.br) on 2017-03-23T12:34:35Z No. of bitstreams: 1 RENAN SERRALVO CAMPOS.pdf: 14804663 bytes, checksum: d21aa4db3ac6aef546c6f30dc795691b (MD5) / Made available in DSpace on 2017-03-23T12:34:35Z (GMT). No. of bitstreams: 1 RENAN SERRALVO CAMPOS.pdf: 14804663 bytes, checksum: d21aa4db3ac6aef546c6f30dc795691b (MD5) Previous issue date: 2017-02-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The high consumption of natural resources and energy, coupled with the significant amount of solid waste generated by the construction activities, makes this sector one of the most responsible for environmental impacts. The recycling of mineral fraction of construction and demolition wastes (CDW), in form of aggregates, is seen as an alternative to reduce these impacts. In this way, the aim of this study was to investigate the influence of recycled aggregates on the rheological, mechanical properties, physical indexes and surface finish of self-compacting concrete (SCC). For this, eight compositions of SCC were analyzed, using the Repette-Melo method and Alfred model, which is based on the concept of particle packing, for the mix design of concretes. Thus, two reference mixtures, composed only of natural aggregates, were established. The other mixtures used recycled fine and/or coarse aggregates instead of natural aggregates, at 20% by mass. The properties of fresh concrete were evaluated with respect to the flowability, viscosity (measure of the speed of flow), passing ability and segregation resistance. In hardened state, compressive strength, splitting tensile strength, static modulus of elasticity, water absorption by immersion and by capillarity, density and voids index were determined. In this study, rheological characterization of cement pastes and mortars destined to the production of SCC was also carried out using rotational rheometry. Rheological characterization of pastes, mortars and concretes is a suitable way to evaluate the behavior of these composites in fresh state, providing useful information that will aid in mix design process and quality control. Another property evaluated was the surface finish of concretes and mortars and the possible correlation of this with the rheological parameters. Through the results obtained it was possible to verify the loss of fluidity in mortars and concretes that used recycled aggregates. Regarding the mechanical properties, different behaviors were observed in concretes produced with recycled aggregates: those that were dosed by Repette-Melo method did not show a significant decrease of these properties; while the concretes dosed by the concept of particle packing exhibited a great reduction in mechanical properties. Concretes produced with recycled aggregates still showed an increase in water absorption by immersion, voids index and capillary absorption, when comparing to the respective reference mixtures. It was possible to observe a good linear correlation between surface finish and shear stress of mortars. In the case of concretes, the slump-flow was the property that best correlated with the quality of surface finish. / O elevado consumo de recursos naturais e energia, associado ao expressivo montante de res?duos s?lidos gerados pelas atividades de constru??o civil faz deste setor um dos maiores respons?veis por impactos ambientais. A reciclagem da fra??o mineral dos res?duos de constru??o e demoli??o (RCD), na forma de agregados, ? vista como uma alternativa para a diminui??o destes impactos. Isto posto, o objetivo deste estudo foi investigar a influ?ncia dos agregados reciclados nas propriedades reol?gicas, mec?nicas, ?ndices f?sicos e no acabamento superficial de concretos autoadens?veis (CAA). Para isso, foram analisadas oito composi??es de CAA, sendo empregados o m?todo de Repette-Melo e o modelo de Alfred, que tem por base o conceito de empacotamento de part?culas, para a dosagem dos concretos. Assim, foram estabelecidos dois tra?os de refer?ncia, compostos apenas por agregados naturais. As demais misturas utilizaram agregados mi?dos e/ou gra?dos reciclados em substitui??o aos agregados naturais, no teor de 20%, em massa. As propriedades do concreto fresco foram avaliadas no que diz respeito ao espalhamento, tempo de escoamento, habilidade passante e resist?ncia ? segrega??o. No estado endurecido foram determinadas as resist?ncias ? compress?o e ? tra??o por compress?o diametral, o m?dulo de elasticidade est?tico, a absor??o de ?gua por imers?o e por capilaridade, a massa espec?fica e o ?ndice de vazios. Neste estudo tamb?m foi realizada a caracteriza??o reol?gica das pastas de cimento e das argamassas destinadas ? produ??o dos CAA?s, por meio de reometria rotacional. A caracteriza??o reol?gica de pastas, argamassas e concretos ? uma forma adequada para avaliar o comportamento destes comp?sitos no estado fresco, podendo prover informa??es ?teis que auxiliar?o no processo de dosagem e controle de qualidade. Outra propriedade avaliada foi o acabamento superficial dos concretos e argamassas e a poss?vel correla??o deste com os par?metros reol?gicos. Atrav?s dos resultados obtidos foi poss?vel constatar perda de fluidez nas argamassas e concretos que empregaram agregados reciclados. No que tange as propriedades mec?nicas, foram observados comportamentos distintos nos concretos produzidos com agregados reciclados: ?queles dosados pelo m?todo de Repette-Melo n?o apresentaram decr?scimo significativo destas propriedades; enquanto que, os concretos dosados com base no conceito de empacotamento de part?culas exibiram grande redu??o nas propriedades mec?nicas avaliadas. Os concretos produzidos com agregados reciclados ainda apresentaram aumento na absor??o de ?gua por imers?o, no ?ndice de vazios e na absor??o capilar, quando comparados aos respectivos tra?os de refer?ncia. Foi poss?vel observar boa correla??o linear entre o acabamento superficial e a tens?o de cisalhamento das argamassas. No caso dos concretos, o di?metro de espalhamento foi a propriedade que melhor se correlacionou com a qualidade do acabamento superficial.

Submitted by Silvana Teresinha Dornelles Studzinski (sstudzinski) on 2016-06-15T14:11:52Z No. of bitstreams: 1 BÁRBARA JORDANI_.pdf: 2132585 bytes, checksum: 322ec6d990fc948ff3060abffa46014a (MD5) / Made available in DSpace on 2016-06-15T14:11:52Z (GMT). No. of bitstreams: 1 BÁRBARA JORDANI_.pdf: 2132585 bytes, checksum: 322ec6d990fc948ff3060abffa46014a (MD5) Previous issue date: 2016-03-31 / FAPERGS - Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / CNPQ - Conselho Nacional de Desenvolvimento Científico e Tecnológico / Nos últimos anos o setor da construção civil está buscando alternativas para substituir as matérias primas utilizadas no processo de produção do concreto, visando preservar o meio ambiente reduzindo extrações da natureza. Diante disso, surge a reciclagem de materiais, que vem ganhando cada vez mais espaço dentro da atividade da construção civil. Uma das alternativas é a utilização de agregados reciclados de concreto (ARC), que podem ser empregados como substituição ao agregado natural. Outra possibilidade é o uso de pozolanas provenientes de resíduos, e dentre as diferentes opções a cinza de casca de arroz se destaca. A cinza de casca de arroz ainda não é largamente utilizada no ocidente, porém vem sendo explorada, sendo um resíduo do setor agropecuário. Após o processo de queima da casca do arroz, surge a cinza que possui grande concentração de sílica. O uso destas pozolanas contribui para diminuir o consumo de cimento. Além disto, o emprego de agregados reciclados pré-saturados pode atuar como um agente de cura interna de concretos, o que, em caso de concretos com pozolanas, pode ser um aspecto positivo para obter-se uma boa hidratação da pasta aglomerante. Este trabalho propõe investigar o efeito do emprego de agregados graúdos reciclados de resíduo de concreto (AGRC) como agente de cura interna em concretos produzidos com pozolana, frente a aspectos vinculados à durabilidade e ao comportamento mecânico. Avaliou-se a substituição do agregado graúdo natural (AN) por (AGRC) em teor determinado pelo Método de Dosagem para Cura Interna, seco e úmido, associado com a substituição de cimento por cinza de casca de arroz em teor de 20%, em dois ambientes de cura (U.R 60% e 100%). Para o estudo foi utilizado três relações a/agl (0,31; 0,42; e 0,53). Os resultados apontaram um efeito positivo do emprego da CCA em concretos fabricados com AGRC. A substituição de cimento por CCA em média pode aumentar a resistência à compressão. Os concretos fabricados com relação a/agl 0,31, para todos os concretos ensaiados, retornaram resistências na faixa de 35-45 MPa. Também foi possível notar retrações significativas até os 28 dias. Já para cura interna cabe ressaltar que o AGRC empregado saturado não apresenta comportamento de agente de cura interna, principalmente no ambiente crítico de ambiente com umidade relativa na ordem de 60%, considerada baixa. / During the last years, the construction sector has been looking for alternatives to replace the raw materials used for concrete production, in order to reduce environmenal impact. The waste recycling as raw material for civil construction can be one of solutions to this problem. One example of this kind of solution is to use the recycled concrete aggregate, which can be used as alternative for the natural aggregate. Another possibility is to use some waste as pozzolanic material, as the rice husk ash. After the burning of the rice hull, it becomes the ash, which has a large concentration of silica. The use of these pozzolans can contribute to reduce the consumption of cement. Furthermore, the usage of pre-saturated recycled aggregate can act as an internal concrete curing agent, which in the case of concretes with pozzolans can be a good way to obtain a better hydration of the binder paste. This study proposes to investigate the effect of the usage of concrete recycled coarse aggregate (RCCA) as an internal curing agent in concrete produced with pozzolan, in order to evaluate the durability and mechanical behaviour. It was evaluated the replacement of natural coarse aggregate (AN) by RCCA. The content of RCCA is related to the binder consumption and it was employed dry and wet. The cement replacement by rice husk ash was done at 20% and it was used three water to binder (w/t) relationships (0.31, 0.42, and 0.53). The curing process was developed in two cure environments (U.R 60% and 100%).The results indicated that the CCA produces positive effect on concrete made with AGRC. The concrete made with respect to w/b 0.31 for all tested concretes returned resistances in the range of 35-45 MPa. The effect of RCCA as internal curing agent is significant when the concrete is cured in a humid environment only for compressive strength. In dry environment, it does not work as internal curing agent. It was also noted significant retractions up to 28 days. As for domestic demand it is noteworthy that the saturated employee AGRC has no internal curing agent behaviour, especially in the critical environment with relative humidity of around 60%, considered low.

A partir de experiências como docente da disciplina de Geografia no Ensino Fundamental e Médio, foi possível analisar as práticas didáticas que contribuem positivamente com o estudo da Geografia em sala de aula, especialmente atividades que envolvem a análise e elaboração de mapas temáticos em conjunto com o conteúdo programático aplicado durante o ano letivo. Neste cenário foi possível elaborar uma pesquisa para o público alvo composto por alunos do 6º e 9º Ano do Ensino Fundamental e 3º Ano do Ensino Superior, com aplicação de atividades e avaliações entre o período de 2010 e 2012 em conjunto com o conteúdo programático de cada período. Esta pesquisa visou analisar o potencial e a viabilidade de aplicação de atividades de construção de mapas temáticos em sala de aula. Para tanto, foram desenvolvidos e adaptados materiais didáticos com recursos alternativos de baixo custo, como radiografias recicladas e papel, além de metodologias para reduzir o tempo de elaboração de mapas em sala de aula. Os critérios de avaliação dos alunos que participaram das atividades foram definidos para avaliar o grau de leitura cartográfica anterior e após a realização das atividades propostas. Para subsidiar a pesquisa, foi feita uma análise bibliográfica sobre a importância da Cartografia no Ensino de Geografia e os fatores que facilitam ou dificultam a realização de atividades práticas durante as aulas. Outro objetivo desta pesquisa foi disponibilizar os materiais aos professores de Geografia para reprodução e/ou adaptação. Assim, os materiais produzidos foram padronizados e apresentados da maneira mais simples possível, a partir de matérias-primas de fácil acesso em qualquer localidade. Os resultados obtidos mostram que após a construção de mapas temáticos em sala de aula, os alunos apresentam melhor rendimento na leitura e interpretação de mapas e temas relacionados à Geografia, especialmente os alunos com maiores dificuldades de aprendizagem. É possível assim, afirmar que a construção de mapas em sala de aula de forma constante é viável, contribui para o ensino de Geografia, e, consequentemente, para a formação escolar de alunos de todos os níveis do Ensino. / From experiences as a Geography teacher in elementary and high school, it was possible to analyze the teaching practices that contribute positively to the study of geography in the classroom, especially activities that involve the analysis and preparation of thematic maps in together with the curriculum implemented during the school year. In this scenario it was possible to develop a survey for the target audience that consists of students from 6th and 9th year of elementary school and 3 Year Higher Education , with application activities and assessments between the period 2010 and 2012 in conjunction with the syllabus of each period. This research aimed to analyze the potential and feasibility of construction activities of thematic maps in the classroom. Thus, we developed and adapted learning materials with low cost alternative resources, such as radiographs and recycled paper, and methodologies to reduce the time of preparation of maps in the classroom. The evaluation criteria of the students who participated in the activities were defined to assess the degree of previous cartographic reading and following completion of the proposed activities. To support the research, an analysis of the literature on the importance of cartography in the Teaching of Geography and the factors that facilitate or hinder the achievement of practical activities during lessons. Another objective of this research was providing materials to teachers of Geography for reproduction and / or adaptation. Thus, the materials produced were standardized and presented in the simplest possible way, from raw materials for easy access from any location. The results show that after the construction of thematic maps in the classroom, students have better performance in reading and interpreting maps and topics related to geography, especially students with major learning difficulties. You can thus say that the construction of maps in the classroom is feasible steadily contributing to the teaching of geography, and, consequently, for the schooling of students from all levels of education.

Les matériaux au contact des aliments (MCDA) constituent un enjeu majeur pour les industriels européens de l’alimentation et de l’emballage d’autant que les MCDA doivent être produits selon les bonnes pratiques de fabrication et être en accord avec les règlementations, notamment le règlement cadre européen 1935/2004. Les MCDA constituent une source potentielle de contamination des aliments car ils peuvent transférer des constituants aux aliments par migration. Parmi les MCDA, les matériaux papiers-cartons sont les plus utilisés après les matériaux plastiques et leur taux de recyclage en Europe était de 72% en 2015 et il ne cesse d’augmenter. En plus des substances dites intentionnelles (SI), des substances non intentionnelles (SNI) peuvent migrer du MCDA vers l’aliment ; elles peuvent être des impuretés, des produits de dégradation, des contaminants provenant des matériaux recyclés etc. Actuellement, de nombreuses SNI ne sont pas identifiées. La présence potentielle de SNI et la nature complexe de ces matériaux rendent difficile l’étude des MCDA papiers-cartons recyclés. Ce projet de thèse a pour objectif d’évaluer le danger lié de ces matériaux. Pour cela, des papiers-cartons recyclés ont été prélevés le long d’une chaine de production (du papier-carton à recycler de début de chaine au papier-carton issu du processus de recyclage), extraits dans l’eau puis lyophilisés. Des analyses chimiques ont été réalisées par spectrophotométrie et par des méthodes chromatographiques (HPLC, GC-MS, LC-MS, ICP-MS). Ces analyses ont montré la présence d’un plus grand nombre de substances dans le papier-carton de début de chaine par rapport au papier-carton de fin de chaine de recyclage. Parmi les composants détectés, des substances naturelles ont été identifiées dans les deux extraits étudiés, provenant notamment du bois qui est la matière première utilisée pour la fabrication du papier (polymères de cellulose, d’hémicellulose et de lignine, acides gras, sucres, acides résiniques ou encore des phénols). D’autres substances identifiées comme additifs ou contaminants ont été caractérisées dans les extraits étudiés, dont bisphénols, des molécules appartenant à la famille des phthalates, des amines aromatiques ou encore des hydrocarbures saturés d’huiles minérales. Il est à souligner qu’une grande partie des substances identifiées dans le produit de début de chaine étaient absentes du produit de fin de chaine. En parallèle des analyses chimiques, des biotests ont été réalisés afin d’identifier des mécanismes d’action et des potentiels effets « cocktail ». Grâce à une batterie de biotests, les effets des extraits sur des cibles toxicologiques ont été étudiés sur différentes lignées cellulaires humaines (deux lignées hépatiques HEpG2 et HepaRG, la lignée hERα-HeLa-9903 et la lignée MDA-kb2): cytotoxicité, génotoxicité et potentiel perturbateur endocrinien. A la plus forte concentration testée, seul l’extrait de produit de fin de chaine a entrainé un effet cytotoxique sur la lignée MDA-kb2. Cependant, les mêmes conditions de test ont montré que les produits de départ et de fin de chaine pouvaient entrainer un stress oxydant dans les lignées hépatiques utilisées. L’évaluation de la génotoxicité a montré des effets génotoxiques des deux papiers-cartons étudiés. Cependant, contrairement aux lésions du matériel génétique induites par le produit de départ, les dommages de l’ADN dus au produit de de fin de chaine pourraient ne pas être réparables pas les systèmes de réparation cellulaires et entrainer des mutations chromosomiques. Enfin, l’étude du potentiel perturbateur endocrinien a mis en évidence des activités oestrogéniques et antiandrogéniques dose-dépendantes des extraits étudiés. Ainsi, la mise en corrélation des résultats d’analyses chimiques et toxicologiques permet d’offrir une stratégie pertinente pour l’évaluation du danger lié aux MCDA, afin d’aider les industriels dans l’évaluation du risque des potentiels NIAS présents dans ces matériaux. / Food contact materials (FCM) are a major issue of concern for European packaging and food companies as FCM must be produced in accordance with the good manufacturing practices and be in compliance with European Regulation 1935/2004/UE. FCM could be a potential source of food contamination because they could release chemicals by a phenomenon called migration. Among FCM, paperboards are the most common materials after plastics and the recycling rate of paperboards is high and was 72% in Europe in 2015. In addition to started substances also called Intentionally Added Substances (IAS), Non-Intentionally Added Substances (NIAS) such as impurities, degradation products, contaminants from recycled materials etc, are also able to migrate from the FCM to food and many of them are not always fully characterized. Assessing the risk of recycled paperboards FCM is difficult because of NIAS and as recycled paperboards can be complex mixtures of substances. This thesis project uses a methodology combining chemical analysis and in vitro bioassays to evaluate the potential hazard of recycled food contact paperboard extracts. For this study, recycled paperboards have been sampled at different steps a food packaging production chain (the starting paperboard of the beginning of the production chain and the end paperboard issued from the recycling process), extracted with water and the dry matter content was recovered by freeze-drying. Chemical analysis of the extracts was performed by spectrophotometry and by chromatographic methods (HPLC, GC-MS, LC-MS and ICP-MS). Chemical analysis showed the presence of a higher number of substances in the starting paperboard than the end paperboard issued from the recycling process. Among these substances, several natural substances were detected in both extracts such as wood extractives (polymers of cellulose, hemicellulose and lignin, fatty acids, sugars, resin acids and phenols). Other substances identified as additives and contaminants were characterized in both extracts such as bisphenols, phthalates, aromatic amins or mineral oil saturated hydrocarbons. It is important to precise that a large number of the substances of the starting paperboard were not detected in the end paperboard. In parallel to chemical characterization, bioassays were used as relevant tools to identify mechanisms of action and potential “cocktail effects”. A battery of bioassays has been performed using different human cell lines (two hepatic cell lines HepG2 and HepaRG, the hERα-HeLa-9903 cell line and the MDA-kb2 cell line) to check toxicological endpoints which are relevant to low exposure: cytotoxicity, genotoxicity and potential endocrine disruption. No cytototoxic effects of both extracts were observed on both human hepatic cell lines HepG2 and HepaRG neither on the HeLa 9903 cell line; whereas the end paperboard extract had cytotoxic effects on MDA-kb2 cells at the highest tested concentration. However, in the same conditions, the starting and the recycled end paperboard extracts were able to induce oxidative stress in the used cell lines. The results of the genotoxicity study showed genotoxic effects of the starting paperboard and the end paperboards. The DNA damage induced by the starting paperboard in the used cellular models might be repaired by cell repair systems. In contrast, data of this study suggest that the end paperboard was able to induce DNA damage that might turn into chromosomal mutations. Data of the endocrine disruption potential showed that the tested FCM paperboard extracts induced significant agonist and dose dependent estrogenic and antiandrogenic activities in the used cell lines. The correlation of the results of the in vitro toxicological study and chemical analysis offers a relevant strategy for hazard assessment of FCM in order to help manufacturers to manage the risk of NIAS.

The use of recycled aggregates in concrete opens a whole new range of possibilities in the reuse of materials in the building industry. This could be an important breakthrough for our society in our endeavours towards sustainable development. The trend of the utilisation of recycled aggregates is the solution to the problem of an excess of waste material, not forgetting the parallel trend of improvement of final product quality. The utilisation of waste construction materials has to be related to the application of quality guarantee systems in order to achieve suitable product properties. Therefore the complete understanding of the characteristics of new material becomes so important in order to point out its real possibilities. The studies on the use of recycled aggregates have been going on for 50 years. In fact, none of the results showed that recycled aggregates are unsuitable for structural use. Only having inadequate number of studies in durability aspects, made recycled aggregates to be preferred just as stuffing material for road construction. My thesis, aimed to focus on the possibility of the structural use of recycled aggregate concrete based on a better understanding of its microstructure.To begin with the characteristics of the aggregates were established, to study their possible application in concrete production. After analysis, the dosage procedure was carried out in order to produce four concrete mixtures using different percentages of recycled coarse aggregates (0% (HC), 25% (HR25), 50% (HR50) and 100% (HR100)) with the same compression strength. Raw coarse aggregates (granite) and sand (crushed limestone) were used in the different concrete mixes.Macroscopic and microscopic examination were carried out in HC, HR25, HR50 and HR100 concretes in order to observe the durability effects. The macroscopic examination determined the aggregates distribution, composition, the contaminants and aureoles around adhered mortar. Microscopic examination was carried out by Optical light transmitted microscope Leica Leitz DM-RXP, using Fluorescence Thin Sections, in order to analyse the cement paste, the new and old interfacial transition zones, secondary reactions as well as damage. Original aggregates and cement paste, interfacial transition zones and alkali silica gel produced due to reactive aggregates present in adhered mortar were analysed by SEM and EDX-maps. Beside macro and micro observations, shear failure behaviour of recycled aggregate concretes was studied. Shear failure test was found more appropriate, as concrete properties had more influence in this type of failure behaviour compared to the flexural failure where the reinforcement plays the important role. Sixteen beam specimens were cast and the structural behaviour of these beams was analysed using four different transversal reinforcements for each kind of concrete. An analytical prediction of the experimental results are carried out using a numerical model (Modified Compression Field Theory), using the codes AASHTO LRFD, CSA, Eurocode-2 and expressions proposed in the Spanish code EHE-99.Organic and inorganic compounds were found to be released from waste materials through leaching and dispersed into the soil and surface water. The leaching of these compounds were measured employing different codes, the two Dutch codes (NEN 7341 and 7345) and the European Normative (EN 12457-2).Some recommendations are given as to the aggregates characteristics to be used in concrete mixes, taking into account the European standards for recycled aggregates. Also suggestions are made for the production process of concrete using recycled aggregate. Mechanical properties of recycled aggregate concrete are studied and they are compared with that of conventional concrete. Based on the durability of the concrete, some suggestions are proposed with respect to possible alkali silica reaction between new cement and original fine aggregates. It is also determined that the effect of the use of recycled aggregate on the beams' shear strength depend on the percentage of coarse aggregate substituted. The applicability of concrete recycled aggregate with respect to its environmental behaviour is demonstrated. In conclusion, some suggestions for future studies are made which would help us in the evolution of our understanding in this field.

Virgin aggregate is being used faster than it is being made available creating a foreseeable shortage in the future. Despite this trend, the availability of demolished concrete for use as recycled concrete aggregate (RCA) is increasing. Using this waste concrete as RCA conserves virgin aggregate, reduces the impact on landfills, decreases energy consumption and can provide cost savings. However, there are still many unanswered questions on the beneficial use of RCA in concrete pavements. This research addresses the many technical and cost-effective concerns regarding the use of RCA in concrete pavements by identifying concrete mixture and proportioning designs suitable for jointed plain concrete pavements; constructing test sections using varying amounts of RCA; monitoring performance through testing, condition surveys and sensor data; modeling RCA pavement performance; and predicting life cycle costs. The research was carried out as a partnership between the Centre for Pavement and Transportation Technology (CPATT) at the University of Waterloo, the Cement Association of Canada, Dufferin Construction, and the Natural Sciences and Engineering Research Council of Canada. The literature review provides an overview of sustainability and key performance indicators, the material properties of RCA both as an aggregate and in concrete, concrete mixture and proportioning designs with RCA, performance of existing RCA pavements, and the implementation of RCA highlighting some examples where RCA has been used successfully. Twelve preliminary mixes were developed using three total cementitious contents amounts of 315 kg/m3, 330 kg/m3, and 345 kg/m3 to determine four suitable mixes with varying coarse RCA contents (0%, 15%, 30% and 50%) to place at the CPATT test track. At 28-days, all of the twelve mixes exceed the 30 MPa design strength. Four test sections containing 0%, 15%, 30% and 50% coarse RCA were constructed in June 2007. The test sections had identical cross sections consisting of 250 mm portland cement concrete (PCC), 100 mm asphalt-stabilized OGDL and a 450 mm granular base. For each coarse RCA content, one slab was instrumented with six vibrating wire concrete embedment strain gages to measure long-term longitudinal and transverse strain due to environmental changes, two vibrating wire vertical extensometers to monitor slab curling and warping, two vibrating wire inter-panel extensometers to monitor joint movement, and two maturity meters to measure maturity and temperature. Quality assurance and quality control (QA/QC) testing showed that the mixes containing RCA exhibited similar or improved performance when compared to the conventional concrete for compressive and flexural strength, freeze-thaw durability and coefficient of thermal expansion. Pavement performance of the four test sections was evaluated using visual surveys following the Ontario Ministry of Transportation’s Manual for Condition rating of Rigid Pavements. Nine pavement evaluations have been performed every two to four months since construction. All test sections are in excellent condition with pavement condition index (PCI) values greater than 85 after two years in-service and approximately three hundred thousand Equivalent Single Axle Loads. Sensor data from the strain gauges, and vertical and inter-panel extensometers are providing consistent results between the test sections. Long-term performance modeling using the Mechanistic-Empirical Pavement Design Guide (ME-PDG) showed improved performance with respect to cracked slabs, joint faulting, and pavement roughness as the RCA content increased. Multivariable sensitivity analysis showed that the performance results were sensitive to CTE, unit weight, joint spacing, edge support, surface absorption, and dowel bar diameter. Life cycle cost analysis (LCCA) illustrated the savings that can be expected using RCA as a replacement aggregate source as the cost of virgin aggregate increase as the sources becomes depleted. Multivariable sensitivity analysis showed that the LCCA results were sensitive to construction costs, discount rate, and maintenance and rehabilitation quantities.

Virgin aggregate is being used faster than it is being made available creating a foreseeable shortage in the future. Despite this trend, the availability of demolished concrete for use as recycled concrete aggregate (RCA) is increasing. Using this waste concrete as RCA conserves virgin aggregate, reduces the impact on landfills, decreases energy consumption and can provide cost savings. However, there are still many unanswered questions on the beneficial use of RCA in concrete pavements. This research addresses the many technical and cost-effective concerns regarding the use of RCA in concrete pavements by identifying concrete mixture and proportioning designs suitable for jointed plain concrete pavements; constructing test sections using varying amounts of RCA; monitoring performance through testing, condition surveys and sensor data; modeling RCA pavement performance; and predicting life cycle costs. The research was carried out as a partnership between the Centre for Pavement and Transportation Technology (CPATT) at the University of Waterloo, the Cement Association of Canada, Dufferin Construction, and the Natural Sciences and Engineering Research Council of Canada. The literature review provides an overview of sustainability and key performance indicators, the material properties of RCA both as an aggregate and in concrete, concrete mixture and proportioning designs with RCA, performance of existing RCA pavements, and the implementation of RCA highlighting some examples where RCA has been used successfully. Twelve preliminary mixes were developed using three total cementitious contents amounts of 315 kg/m3, 330 kg/m3, and 345 kg/m3 to determine four suitable mixes with varying coarse RCA contents (0%, 15%, 30% and 50%) to place at the CPATT test track. At 28-days, all of the twelve mixes exceed the 30 MPa design strength. Four test sections containing 0%, 15%, 30% and 50% coarse RCA were constructed in June 2007. The test sections had identical cross sections consisting of 250 mm portland cement concrete (PCC), 100 mm asphalt-stabilized OGDL and a 450 mm granular base. For each coarse RCA content, one slab was instrumented with six vibrating wire concrete embedment strain gages to measure long-term longitudinal and transverse strain due to environmental changes, two vibrating wire vertical extensometers to monitor slab curling and warping, two vibrating wire inter-panel extensometers to monitor joint movement, and two maturity meters to measure maturity and temperature. Quality assurance and quality control (QA/QC) testing showed that the mixes containing RCA exhibited similar or improved performance when compared to the conventional concrete for compressive and flexural strength, freeze-thaw durability and coefficient of thermal expansion. Pavement performance of the four test sections was evaluated using visual surveys following the Ontario Ministry of Transportation’s Manual for Condition rating of Rigid Pavements. Nine pavement evaluations have been performed every two to four months since construction. All test sections are in excellent condition with pavement condition index (PCI) values greater than 85 after two years in-service and approximately three hundred thousand Equivalent Single Axle Loads. Sensor data from the strain gauges, and vertical and inter-panel extensometers are providing consistent results between the test sections. Long-term performance modeling using the Mechanistic-Empirical Pavement Design Guide (ME-PDG) showed improved performance with respect to cracked slabs, joint faulting, and pavement roughness as the RCA content increased. Multivariable sensitivity analysis showed that the performance results were sensitive to CTE, unit weight, joint spacing, edge support, surface absorption, and dowel bar diameter. Life cycle cost analysis (LCCA) illustrated the savings that can be expected using RCA as a replacement aggregate source as the cost of virgin aggregate increase as the sources becomes depleted. Multivariable sensitivity analysis showed that the LCCA results were sensitive to construction costs, discount rate, and maintenance and rehabilitation quantities.

Includes vita. / Title from PDF of title page. / Document formatted into pages; contains 329 pages. / Thesis (Ph.D.)--University of South Florida, 2003. / Includes bibliographical references. / Text (Electronic thesis) in PDF format. / ABSTRACT: River discharge leads to extensive phytoplankton blooms often observed in ocean color satellite images to extend far into the open ocean as high chlorophyll plumes. We investigated diversity, distribution and ecology of phytoplankton populations in the Mississippi River plume, both spatially and in the water column using molecular tools. A method was developed for the quantification of diatom/pelagophyte rbcL (large subunit of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase) mRNA using quantitative PCR and applied to cultures and in the plume. The vertical structure of phytoplankton species in the Mississippi River plume was described by flow cytometry, pigments, rbcL mRNA and rbcL cDNA libraries. High productivity in the plume was associated with a large population of Synechococcus and elevated levels of cellular form IA rbcL mRNA. / ABSTRACT: rbcL cDNA libraries indicated two vertically separated clades of Prochlorococcus (high-light and low-light adapted) in addition to a diverse group of prymnesiophytes and a microdiverse clade of prasinophytes, which may have dominated the SCM (Subsurface Chlorophyll Maximum). In situ sampling and satellite image analysis were used to estimate that the plume accounted for 41% and 13% of all surface water column ix productivity in the oligotrophic Gulf of Mexico, while covering less than 3% of its area. Coastally the plume is dominated by diatoms, which are replaced by a bloom of Synechococcus as the plume moves offshore. Diatoms as indicated by pigments and rbcL clone libraries again dominated the offshore, least productive plume. 15N uptake measurements indicated that rapid recycling of ammonium despite higher levels of nitrate primarily drives production in the offshore plume. / ABSTRACT: rbcL mRNA levels and photosynthetic capacity displayed strong diel patters in three out of four time series sampled during the GRIST (Geochemical Rate/mRNA Integrated Study). In addition it was demonstrated that transcriptional regulation of the global nitrogen regulatory protein NtcA in Synechococcus WH7803 may involve a small cis-encoded anti-sense mRNA. Methods for the generation of large insert BAC (Bacterial Artificial Chromosome) from cultures and the environment were refined. Partial sequencing and genomic comparison of an ntcA containing BAC clone obtained from Synechococcus WH7803 indicated that ntcA is not part of a larger nitrogen assimilation operon in cyanobacteria. / System requirements: World Wide Web browser and PDF reader. / Mode of access: World Wide Web.

Detta examensarbete omfattar produktutvecklingen av en komponent till en mätutrustning som ska mäta flytbarheten hos smält metall. Arbetet har utförts hos Bryne AB och har lett fram till koncept-, material- och tillverkningsförslag för komponenten. Arbetet har även utvärderat ett för gjuteribranschen nytt formmaterial som alternativ till dagens gjutsand. Tester har gjorts för att se om återvunnet (material x) går att använda som formmaterial till högtemperaturapplikationer. / This thesis covers the product development of a component for a measuring device that measures the fluidity of molted metal. The work has been performed at Bryne AB and has led to concept, material and manufacturing proposal for the component. This work has also evaluated new molding material to be used in foundries that can be an alternative to today's molding sand. Tests have been made to see if recycled (material x) can be used as mold material for high temperature applications. / <p>Uppladdad version av rapporten är en offentlig kopia av orginalrapporten. I den offentliga versionen har känslig information fått ett annat namn för att inte påverka uppdragsgivarens patentansökan.</p>