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Fosfolipases D (FLD) do veneno das aranhas do gênero Loxosceles são capazes de causar entre outros efeitos, uma forte agregação plaquetária cujo mecanismo ainda não foi elucidado. Portanto, para estudar o papel das FLDs nesta atividade, uma FLD recombinante de L. gaucho (LgRec1) foi fusionada com a proteína fluorescente verde (EGFP) e utilizada como uma sonda para detectar a interação de LgRec1 com plaquetas. Essa quimera, denominada EGFP-LgRec1, manteve as principais características da LgRec1. A microscopia confocal das plaquetas mostrou que LgRec1 não requer componentes plasmáticos para se ligar às plaquetas, embora estes sejam necessários para que a LgRec1 induza agregação. Além disso, foi observado que a ação da LgRec1 leva à exposição de fosfatidilserina. Contudo, esta exposição não está relacionada à morte celular. Portanto, este trabalho mostrou que uma FLD de Loxosceles se liga a plaquetas, promovendo a exposição de fosfatidilserina, possibilitando a ligação de fatores de coagulação e resultando na agregação plaquetária. / Phospholipases D (PLD) from spider venom of the genus Loxosceles are capable of causing, among other effects, a strong aggregation of platelets and its mechanism has not yet been elucidated. Therefore, to study the role of PLDs in this activity, a recombinant L. gaucho PLD (LgRec1) was fused with a green fluorescent protein (EGFP) and used as a probe to detect the interaction of LgRec1 with platelets. This chimera, named EGFP-LgRec1, remained the main activities of LgRec1. Platelet confocal microscopy has shown that LgRec1 does not require plasma components to bind to platelets, although these are required for LgRec1 to induce aggregation. In addition, it has been observed that the action of LgRec1 leads to exposures of phosphatidylserine. However, this exposure is not related to cell death. Therefore, this work showed that a Loxosceles PLD binds to platelets, promoting an exposure of phosphatidylserine, that may act as a scaffold for coagulation factors, resulting in platelet aggregation.

In Italy, the period directly following World War II was marked by confusion and turbulence as the people struggled to reconstruct both the ideological and physical infrastructure of the nation. While much study has been dedicated to the evolution of femininity and the figure of the woman in this particular period, comparatively little has been written on the refashioning of masculinity in the texts produced in the period between 1940 and 1955. After the fall of the Fascist Regime, Italian masculinity undergoes a drastic transformation as the generation of young men born and raised under the tutelage of Mussolini’s reign attempt to separate themselves from the now-tainted codes of conduct governing male behavior. This report analyzes the renegotiation of Italian masculinity in G. Silvano Spinetti’s non-fictional account Difesa di una generazione (scritti e appunti), Italo Calvino’s Il sentiero dei nidi di ragno, Beppe Fenoglio’s short story “Gli inizi del partigiano Raoul” and Pier Paolo Pasolini’s Ragazzi di vita. These works, written and published in the postwar period, manipulate the vi marginality and privation experienced by the Italian population during the war and postwar period into a liminal state brimming with revolutionary potentiality. The protagonists of these texts (both fictional and non-fictional), isolated from the larger social context and deprived of individual identity, property and privilege, circumvent their polluted patriarchal lines in favor of an alternative ideological patriarchy. While Spinetti, Calvino and Fenoglio’s works advance their liminal narratives as a means of creating an emblematic Italian man capable of rejoining the generative discourse, Pasolini’s text renounces such a progressive view. In Ragazzi di vita, the only possibility for a masculine identity free of Fascism resides in a maintaining a perennial liminality. / text

Au cours de ce travail, nous nous sommes intéressés à l’influence des conditions locales et paysagères sur la structuration des communautés d’arthropodes en agroécosystèmes, ceci grâce à un dispositif de suivi situé en Ille-et-Vilaine (Bretagne, France), et comprenant des parcelles conduites en agriculture biologique et conventionnelle spatialement appariées. Notre première étude a mis en évidence que l’hétérogénéité paysagère, parfois en interaction avec le système local de culture, conditionne la diversité et l’abondance des groupes trophiques, à la fois pour les arthropodes au sol comme pour ceux de la végétation. Dans un second temps, nous avons pu montrer l’importance du contexte paysager dans la structuration des assemblages d’arthropodes prédateurs, et émis l’hypothèse que les mécanismes déterminant la distribution des espèces diffèrent fortement entre araignées et carabiques. Enfin, il est apparu la nécessité de distinguer les individus résidents (émergeants) des individus mobiles (circulants) lorsque l’on s’intéresse au rôle différentiel des facteurs locaux vs. paysagers dans la structuration des communautés de carabiques. D’une façon générale, nos résultats montrent donc une influence importante et positive de l’agriculture biologique sur les populations, assemblages d’espèces et communautés d’arthropodes, que ce soit à des échelles locales et paysagères comme en interaction avec d’autres variables paysagères. Nous avons également montré la pertinence de l’utilisation de différents niveaux d’organisation et variables réponses associées pour évaluer finement la structure et le fonctionnement de communautés d’Arthropodes en agroécosystèmes. / In this study, we got interested in quantifying how local and landscape metrics structure arthropod communities in agro-ecosystems. For that, we used a long-term monitoring design located in ‘Ille-Et-Vilaine’ (Brittany, France), composed by spatially pair-matched fields under organic vs. conventional farming systems. First, we found that landscape heterogeneity, also interacting with farming systems, drives the diversity and abundance of trophic groups for both ground- and vegetation-dwelling arthropods. In a second chapter, we showed the importance of landscape context in shaping assemblages of predatory arthropods, and suggested that mechanisms behind the distribution of individual species strongly differ between spiders and carabids. Lastly, we highlighted the importance of distinguishing between resident (emergent) and mobile (circulating) individuals when assessing the differential role of local vs. landscape factors in community assembly. Overall, our results show a strong and positive effect of organic farming on arthropod populations, assemblages and communities, both at local and landscape scales, as well as in interaction with other landscape metrics. We also highlighted the relevance of using different levels of biological organization, and related response variables, when assessing the structure and functioning of arthropod communities in agroecosystems

Fosfolipases D (FLD) do veneno das aranhas do gênero Loxosceles são capazes de causar entre outros efeitos, uma forte agregação plaquetária cujo mecanismo ainda não foi elucidado. Portanto, para estudar o papel das FLDs nesta atividade, uma FLD recombinante de L. gaucho (LgRec1) foi fusionada com a proteína fluorescente verde (EGFP) e utilizada como uma sonda para detectar a interação de LgRec1 com plaquetas. Essa quimera, denominada EGFP-LgRec1, manteve as principais características da LgRec1. A microscopia confocal das plaquetas mostrou que LgRec1 não requer componentes plasmáticos para se ligar às plaquetas, embora estes sejam necessários para que a LgRec1 induza agregação. Além disso, foi observado que a ação da LgRec1 leva à exposição de fosfatidilserina. Contudo, esta exposição não está relacionada à morte celular. Portanto, este trabalho mostrou que uma FLD de Loxosceles se liga a plaquetas, promovendo a exposição de fosfatidilserina, possibilitando a ligação de fatores de coagulação e resultando na agregação plaquetária. / Phospholipases D (PLD) from spider venom of the genus Loxosceles are capable of causing, among other effects, a strong aggregation of platelets and its mechanism has not yet been elucidated. Therefore, to study the role of PLDs in this activity, a recombinant L. gaucho PLD (LgRec1) was fused with a green fluorescent protein (EGFP) and used as a probe to detect the interaction of LgRec1 with platelets. This chimera, named EGFP-LgRec1, remained the main activities of LgRec1. Platelet confocal microscopy has shown that LgRec1 does not require plasma components to bind to platelets, although these are required for LgRec1 to induce aggregation. In addition, it has been observed that the action of LgRec1 leads to exposures of phosphatidylserine. However, this exposure is not related to cell death. Therefore, this work showed that a Loxosceles PLD binds to platelets, promoting an exposure of phosphatidylserine, that may act as a scaffold for coagulation factors, resulting in platelet aggregation.

Les carabes et les araignées sont des auxiliaires généralistes susceptibles d'exercer un contrôle efficace des ravageurs des cultures. La structure et composition de leurs communautés, ainsi que l'efficacité du contrôle biologique, dépendent de l'hétérogénéité des paysages, et en particulier de la présence d'habitats semi-naturels. Mais le rôle de la mosaïque agricole, susceptible de présenter une forte hétérogénéité dans l'espace et dans le temps, reste aujourd'hui peu connu. L'objectif de ce travail a été d'évaluer les effets de l'hétérogénéité spatiale et temporelle des paysages agricoles sur des auxiliaires généralistes et sur le potentiel de contrôle biologique. Nous avons échantillonné les communautés de carabes et d'araignées dans des parcelles de céréales, et estimé le potentiel de prédation par des pucerons sentinelles collés sur des cartes de prédation. Nous avons caractérisé l'hétérogénéité spatiale des paysages autour des parcelles de céréales par le pourcentage d'éléments boisés et la longueur du réseau de haies, la taille moyenne des parcelles agricoles, et la diversité des cultures. Nous avons également mis au point quatre nouvelles métriques qui synthétisent différents aspects de l'hétérogénéité temporelle interannuelle des surfaces cultivées. Nos résultats montrent qu'au printemps les araignées sont plus abondantes dans les parcelles de céréales situées dans des paysages composés de petites parcelles. Au début de l'été, ces paysages favorisent également les taux de prédation de pucerons mesurés par les cartes de prédation, et l'abondance des carabes qui se reproduisent au printemps et qui hivernent en tant qu'adultes dans des habitats semi-naturels. Les carabes qui hivernent dans le sol des parcelles au stade larvaire et se reproduisent à l'automne sont quant à eux favorisés par la diversité des cultures dans le paysage. Nos résultats montrent enfin que l'hétérogénéité temporelle de la mosaïque agricole - caractérisée par les changements de la diversité de cultures au cours des cinq dernières années - favorise elle aussi certaines espèces de carabes communément rencontrées dans les parcelles agricoles (Poecilus cupreus ou Pterostichus melanarius) et susceptibles de jouer un rôle important en tant qu'auxiliaires. Ces résultats mettent en avant le rôle complémentaire des bordures de champs non cultivées et de la mosaïque des cultures pour différents groupes d'auxiliaires, et soulignent l'importance de prendre en compte l'hétérogénéité spatiale et temporelle des surfaces cultivées dans l'étude de la biodiversité des paysages agricoles. Dans le contexte de réduction des produits phytosanitaires, nos résultats suggèrent que des paysages présentant une diversité de cultures importante variable dans le temps, et des petites parcelles qui favorisent l'intrication entre les habitats semi-naturels et les cultures, sont susceptibles de favoriser les auxiliaires généralistes ainsi que le potentiel de contrôle biologique des ravageurs. / In the context of reducing pesticide use, the potential role of some arthropod groups as pest natural enemies provides them an ecological and economic interest. In particular, ground beetles and spiders are generalist predators likely to be effective biocontrol agents. Previous studies have shown that the structure and composition of their communities, as well as the effectiveness of biological control, are influenced by landscape heterogeneity, and in particular by the presence of semi-natural habitats. However, the role of the crop mosaic, which can be highly heterogeneous in space and time, have been little considered. The main objective of our study was to determine the influences of spatial and temporal heterogeneity of agricultural landscapes on generalist natural enemies and the potential for biological pest control. We sampled carabid and spider communities in winter cereal fields, and estimated biological control potential using sentinel aphids glued on predation cards. We characterized the spatial heterogeneity of the landscapes around each cereal field by 1) the amount of wooded habitats and the length of the hedgerow network, 2) the average size of the agricultural fields, and 3) crop diversity. We also proposed four new metrics that synthesize different aspects of the multi-year temporal heterogeneity of cropped areas. Our results show that in spring, landscapes characterized by small fields, and therefore by a high density of non-cropped field margins, promote spiders abundance in cereal fields. In early summer, landscapes with small fields also promote aphid predation rates and the abundance of carabid spring breeders, which overwinter as adults in semi-natural habitats. Crop diversity promotes the abundance of carabids overwintering in soils of arable fields and breeding in autumn. Our results also show that the temporal heterogeneity of the crop mosaic - characterized among other things by the changes in crop diversity over a five year period - benefits some carabid species commonly found in agricultural landscapes (such as Poecilus cupreus and Pterostichus melanarius) which are likely to play an important role as pest natural enemies. These results highlight the complementary role of the crop mosaic and the non-cropped field borders for generalist natural enemies, and show the importance of taking into account the spatial and temporal heterogeneity of the cultivated area in further ecological studies on biodiversity in agricultural landscapes. In the context of reducing pesticide use, our results suggest that landscapes with 1) a high crop diversity that change over time, and 2) small fields that promote the interspersion between crops and semi-natural habitats, are likely to promote generalist natural enemies and biological pest control.

No description available.