Detta examensarbete har varit att ta fram ett koncept för ett second-screenlager till företaget Headwebs filmuthyrningstjänst. Denna rapport visar på hur tre second-screenfunktioner kan appliceras i en video on demand-tjänst. De tre funktionerna som applicerades var att som användare via sin second-screen välja på vilken enhet man vill se sin film, kontrollera filmens playbackkontroller och välja ut små filmklipp från filmen och dela dessa på sociala medier. Resultatet blev ett lo-fi-prototyp som visar hur de tre funktionerna kan appliceras på Headwebs iPadtjänst och listar för- och nackdelar med de olika konceptet. / This thesis has been about developing a second screen layer for the company Headwebs movie rental service. This report shows how three second screen features can be applied in a video on demand service. The three functions that was applied was as a user to be able through its second screen choose which device you want to see the movie, control the movie's playback controls and select small movie clips from the movie and share them in social media. The result was a lo-fi prototype that shows how the functions can apply to Headweb iPadservice and lists the advantages and disadvantages of the various functions.

Utveckling av mobila applikationer är idag allt svårare för utvecklare, eftersom det finns många olika enheter och operativsystem. Detta examensarbete kommer gå igenom hur multiplattformsramverk kan användas för att utveckla och implementera en second screen som komplement till andra enheter. Plattformarna kommer utvärderas med avseende på hur komplext ded är att implementera lösningen samt hur de presterar under olika förhållanden. De multiplattformsramverk som kommer utvärderas är OpenFL och PhoneGap. / Development of mobile applications today are increasingly difficult for developers, because there are many different devices and operating systems. This thesis will look at how a cross-platform framework can be used to develop and implement a second screen as a complement to other devices. he platforms will be evaluated in terms of how complex it is to implement the solution and how they perform under different conditions. The cross-platform frameworks that will be evaluated is OpenFL and PhoneGap.

These days, everything revolves around technology, and we have instantaneous access to all information. To stay up to date on current events, we get notifications on our smart devices when relevant articles or messages become available. Displays and other forms of technology are ubiquitous in today's car interiors to both enhance the driving experience and keep the driver in touch with the information they provide. However, depending on the situation and task at hand, these notifications can elicit a variety of reactions from us when they appear at an inconvenient time. The problem statement of this research was that there was very little amount of research done on the connection between drivers with different personality traits and how the incoming in-vehicle notifications affect them in various cognitive load scenarios. This study put participants in a practical research where they have been doing a primary task while interacting with notifications as a side task. The tasks at hand have been divided based on the user's cognitive load, or the amount of focus required for that activity, like different driving scenarios in real life. We have collected sufficient data through practical research in which participants have been placed in a scenario of performing a primary task while interacting with incoming notifications. Prior to that, the personality traits of the participants have been determined using a survey, which assisted us in connecting the similarities and differences among the various personality trait groups. In the practical part of the study, a screen was used on which the participant performed a primary task in the form of a video game, as well as a screen beside him, mimicking an infotainment screen, with incoming notifications with which he had to interact. This provided us with accurate data based on its similarity to an actual everyday scenario, such as while driving a car. The findings aided in establishing a clear link between personality traits, levels of cognitive load, and notifications in general, and how they are interlinked.

The problem with which this comparative investigation is concerned is the application of a photographically derived image to glass. The image used originated from an ordinary thirty-five-millimeter color slide. This slide, through photographic darkroom manipulation, was translated into thirty individually different, black and white films of four-by-five-inch size. Selected films were then enlarged onto eleven-by-fourteen-inch, Kodalith film. These enlarged films were contact exposed to Ulano's Blue Poly-3, a presensitized silkscreen photofilm. This in turn was adhered to twelve double X silk which was tautly stretched in a wooden frame.

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Building upon previous research done on interactivity attributes describing the aesthetic quality of interactions, this paper aims to explore the sense of ow in screen-based interactions, narrowing down to the finer details that make the personality of the interactive experience. The goal is to provide a deeper knowledge on the concrete, expressive qualities of screen based interaction, being “the immaterial material” moulded in the design process. The relevant information for practicing designers is the awareness of what emotions certain interactions elicit and how interactions can be used to convey brand personality.The process conducted followed a research through design methodology, as the aim was to explore the defined design space rather than answer a specific problem. Two major user studies provided insights and refocus in the process along the way to the final result: a set of guidelines and a prototype embodying them. The guidelines provide some details on designing flow experiences on a mobile screen, and are to serve as inspiration and reference to future work and design practice. The prototype, called ‘The Embodiment’ serves as an illustration of the guidelines, as mere words are not able to fully describe the dynamic quality.

A presente dissertação de mestrado tem o propósito de relatar o desenvolvimento de um sensor planar eletroquímico com três eletrodos para o monitoramento de nitrito e seus interferentes (ácido úrico, ácido ascórbico e paracetamol). Para tal, no procedimento experimental é descrito o desenvolvimento do aparato extracorpóreo (mini-bomba compressora, válvulas solenóides e cela de análise). Esse é acoplado a um sistema de Análise por Injeção em Fluxo Automatizada (do inglês, Flow-injection Analysis) FIA-automatizada. Este trabalho também descreve a fabricação dos sensores em substrato de alumina, o processo de limpeza padrão dos eletrodos, o processo de obtenção dos eletrodos de referência em HCl (0,1 mol L-1) e ativação eletroquímica da superfície do eletrodo de trabalho com H2SO4 (0,1 mol L-1). Em seguida, é mostrada a estabilização dos eletrodos de referência Ag/AgCl em solução salina contendo cloretos, o estudo da secagem dos eletrodos em temperatura ambiente com exposição à luz e a caracterização dos mesmos por três métodos distintos, a saber: i) teste de reversibilidade (corrente redox vs. potencial); ii) variação do potencial com o tempo (coeficiente de degradação termodinâmica) e iii) variação do potencial com a concentração de cloretos. Também, apresenta-se a caracterização da área efetiva exposta do eletrodo de medição (trabalho) e os resultados da deposição de 1-2 Diaminobenzeno (DAB) como polímero seletivo. Além disso, são apresentados os resultados e discussões dos pseudo-eletrodos (recoberto ou não com Náfion® 117). Um ponto relacionado, a histerese associada à irreversibilidade dos eletrodos de referência resultou da ordem de 40 mVAg/AgCl Náfion® 117. Os potencias termodinâmicos observados foram da ordem de 100 ± 6 mVAg/AgCl Náfion® 117 para pseudo-eletrodo de referência sem e com Náfion® 117. Depois, foi realizado um estudo criterioso dos potenciais de resposta ao nitrito e aos interferentes por meio da técnica de DPV (do inglês, Differencial Pulse Voltammetry). Para evitar degradação acelerada (corrosão) do polímero sobre o eletrodo de trabalho, optou-se por realizar as medições com potencial igual a 0,50 VAg/AgCl Náfion® 117. Nessa condição, apresenta-se a monitoração de nitrito por FIA-automatizada na faixa de concentração de 50 a 250 mol L-1. Os resultados para a medição de nitrito mostraram que o sensor planar eletroquímico amperométrico desenvolvido é promissor, pois esse apresentou ótimo desempenho de reprodutividade (99,66%), sensibilidade (90 microA mol-1 L mm-2), seletividade (0,32%), repetitividade (91,28%) e estabilidade (15 ± 0,3 pA). / In this Master of Science, it has been developed an electrochemical planar sensor defined with three electrodes for nitrite measuring and its interferents (uric acid, ascorbic acid and paracetamol). In the experimental procedure, it is shown the development of an extracorporis set up (mini-pump, solenoid valves and analysis cell) which is coupled to a system of Flow-injection Analysis (FIA). This work also describes the sensor fabrication using alumina substrates, the standard cleaning of the electrodes, the process to obtain Ag/AgCl reference electrodes in HCl (0.1 mol L-1) and electrochemical activation of the working-electrode surface with H2SO4 (0.1 mol L-1). Following, it is shown the study of drying for electrodes at room temperature with exposition to room light and their characterization using three different methods: i) reversibility test (redox current vs. potential); ii) variation of the potential with the time (thermodynamics degradation coefficient) and iii) variation of the potential with the chloride concentration. Also, it is presented the characterization of the effective exposed area of the working electrode and the results of the deposited 1-2 Diaminobenzene (DAB) is presented as a selective polymer. In addition, the results and discussions of the pseudo-reference (recovered or not with Nafion® 117) are presented. Related to irreversibility is the hysteresis associated to the reference electrodes which resulted in the order of 40 mVAg/AgCl Nafion® 117. The observed thermodynamic potentials were of (100 ± 6) mVAg/AgCl Nafion® 117 for pseudo-electrodes recovered with Nafion® 117. Later on, a careful study of the response to nitrite and to its interferents was performed by means of the DPV technique (Differential Pulse Voltammetry). In order to avoid accelerated degradation (corrosion) of the polymer, measurements were performed at a potential of 0,5 VAg/AgCl Nafion® 117. In this condition, nitrite was monitored using the automatized-FIA (Flow Injection Analysis) for concentration in the range of 50 to 250 mol L-1. The results have shown that the planar amperometric sensor for nitrite measuring is usefull since it was observed excellent performance related to reproducibility (99.66%), sensitivity (90 microA mol-1 L mm-2), selectivity (0.32%), repetibility (91.28%) and stability (15.0 ± 0.3 pA).

Le réseau de signalisation qui répond à l’insuline et aux nutriments est conservé chez les métazoaires, où il joue un rôle central dans le contrôle du métabolisme et de la croissance. Les nutriments assimilés sont soit directement utilisés pour la croissance tissulaire, soit stockés principalement sous forme de triglycérides. Chez la drosophile, l’activation de ce réseau de signalisation dans le corps gras, un organe qui remplit à la fois les fonctions hépatiques et destockage, induit une augmentation du stockage de lipides sous forme de nombreuses gouttelettes lipidiques (LDs). A l’inverse, la carence alimentaire se traduit par une augmentation de la taille des LDs et une diminution de lipides stockés. La kinase TOR (TargetOf Rapamycine) et son substrat S6 Kinase (S6K) jouent un rôle central dans cette régulation.Chez la drosophile, ces 2 kinases (dTOR et dS6K) contrôlent les aspects autonome-cellulaireset hormonaux de la croissance. En dépit de nombreuses études sur divers organismes modèles,destinées à comprendre les mécanismes régulateurs de S6K, rien n’est connu à ce jour sur lecontrôle de sa dégradation.Nous avons utilisé une banque de lignées exprimant des ARN interférant (RNAi) contre unegrande quantité de gènes de la drosophile, pour réaliser 3 des cribles génétiques destinés à identifier de nouveaux régulateurs du métabolisme et de la croissance. Dans le premier crible,les RNAi ont été induits dans la glande prothoracique, siège de la production de l’hormonestéroïde ecdysone connue pour réguler la croissance et les étapes du développement, souscontrôle de la nutrition et de la signalisation dTOR. Sur 7000 gènes criblés, 620 ont étéidentifiés comme nécessaire à la production d’ecdysone. Dans le second crible, nous avonsexprimé les RNAi de 4000 gènes dans le corps gras pour rechercher ceux qui induisaient uneaugmentation de la taille des LDs. L’objectif était d’identifier des gènes impliqués dans la réponse à la carence alimentaire, et nous avons ainsi retenu 24 candidats intéressants. Le troisième crible représente la majeure partie du travail de thèse, où nous avons criblé les RNAi susceptibles de modifier un phénotype de croissance induit par dS6K. Sur 7000 gènes testés,nous en avons retenu 45 qui ont ensuite été utilisés pour générer un diagramme d’interaction en utilisant les informations disponibles dans les banques de données. Les candidats les plus intéressants ont ensuite été analysés en culture de cellules pour identifier ceux qui régulent l’activité de dS6K et ceux qui régulent sont niveau d’expression. Parmi ces derniers, nousavons identifié le gène codant pour Archipelago (Ago), connue pour contrôler la dégradationrégulée des protéines-cibles au niveau du protéasome. Nous avons réalisé de nombreusesexpériences qui montrent que ago et dS6K interagissent génétiquement. En outre, il est indiquédans les banques de données que ces protéines interagissent entre elles par la technique des 2-hybrides en levure. Tous ces résultats révèlent que Ago régule la dégradation de dS6K, etposent les premières pierres de ce niveau de régulation. / The evolutionary conserved insulin and nutrient signaling network regulates growth andmetabolism. Nutrients are directly utilized for growth or stored, mostly as triglycerides. InDrosophila, activation of insulin/nutrient signaling in the fat body (the fly equivalent of liverand adipose tissue), causes an increase in fat stores composed of several small-size lipiddroplets (LDs). Conversely, fasting produces an increase in LD size and a decrease in fatcontents. The TOR kinase and its substrate S6 kinase (S6K) play a central role in this response,and particularly in Drosophila, they have been shown to orchestrate cell-autonomous andhormone-controlled growth. However, despite extensive research studies on different modelorganisms (mouse, fly, worm) to decipher the molecular and physiological functions of S6K,nothing is known about how its degradation is regulated.Taking advantage of the inducible RNA interfering (RNAi) library from NIG (Japan), we haveperformed three genetic screens to identify novel regulators of steroidogenesis, lipidmetabolism and dS6K-dependent growth. First, RNAi lines were screened in the ring gland; anorgan that controls the progression of the developmental steps by producing the steroidhormone ecdysone. Out of 7,000 genes screened, 620 positive candidates were identified toproduce developmental arrest and/or overgrowth phenotypes. Then, we challenged 4,000 genesby RNAi screening able to recapitulate the larger sized LD phenotype as obtained uponstarvation, leading to the identification of 24 potential candidates. Finally, the RNAi lines werescreened for their ability to enhance a growth phenotype dependent of the Drosophila S6K(dS6K). Out of 7,000 genes screened, 45 genes were identified as potential negative regulatorsof dS6K. These genes were further used to design a novel protein-protein interaction networkcentered on dS6K through the available data from yeast-2-hybrid (Y2H) assay. The most potentinteractors were then analyzed by treatment of cultured S2 cells with the corresponding doublestrand RNA (dRNA). Western blotting thus, allowed us to discriminate between the geneproducts that regulate dS6K levels versus those that regulate its phosphorylation, as a hallmarkfor its kinase activity. Interestingly, archipelago (ago), which encodes a component of an SCFubiquitinligase known to regulate the degradation of dMyc, Cyclin E and Notch, was identifiedas a negative regulator of dS6K-dependent growth. Based on the Y2H available data showingthat Ago and dS6K interact each other and the presence of a putative Ago-interaction motif indS6K, we hypothesized that Ago causes an ubiquitin-mediated degradation of dS6K. Ourmolecular data showed that loss of ago caused an elevated level of dS6K, which confirms arole of Ago in controlling dS6K degradation. Altogether our findings emphasize the importanceof the saturating screening strategies in Drosophila to identify novel regulators of metabolicand signaling pathways.

Bakterielle Infektionen aktivieren den Transkriptionsfaktor NF-κB. Dies trägt sowohl zur Immunantwort, als auch zum Überleben der Wirtszellen bei. In Infektionen mit dem Bakterium Helicobacter pylori stellt diese doppelte Funktion einen mechanistischen Link zwischen der chronischen Entzündung und der Krebsentstehung dar. Für das intrazelluläre Bakterium Legionella pneumophila ist das Überleben der Wirtszelle notwendig für die Vermehrung. Bisher sind jedoch bei beiden Infektionen die Signalwege, die zur NF-κB-Aktivierung führen, nicht ausreichend untersucht. Um NF-κB analysieren zu können, wurden hier monoklonale Zelllinien hergestellt, die ein Fusionsprotein der NF-κB-Untereinheit p65 mit GFP stabil exprimieren. Die Kerntranslokation von p65-GFP kann mit Fluoreszenz-Mikroskopie visualisiert und mit automatischer Bildanalyse quantifiziert werden. Mit dieser Methode konnte zum ersten Mal eine durch ein Bakterium induzierte Oszillation von p65-GFP gezeigt werden. In einem RNAi-basierten Hochdurchsatzscreen wurde diese Methode eingesetzt um neue Faktoren im NF-κB-Signalweg zu identifizieren. Dabei wurde die Infektion mit H. pylori mit den Induktoren TNFα und IL-1β verglichen. Insgesamt wurden 24 Regulatoren identifiziert. Die Identifikation dieser Faktoren vertieft nicht nur unser Verständnis des NF-κB-Signalweges, sondern bietet auch neue molekulare Ziele für mögliche zukünftige Therapien. Bei der detaillierten Analyse der L. pneumophila-induzierten NF-κB-Aktivierung konnte ein einzigartiger, zwei-phasiger Ablauf gezeigt werden. Zunächst verursachte bakterielles Flagellin eine starke, aber kurze Aktivierung. Später war p65 dauerhaft über Stunden im Kern lokalisiert, was eng an die Replikation der Bakterien gekoppelt war. Da die kontinuierliche Kernlokalisation für Transkriptionsfaktoren der NF-κB Familie sehr ungewöhnlich ist, könnte dies ein Hinweis für eine Manipulation durch das Bakterium sein, um das Überleben der Wirtszelle zu sichern. / Infection with pathogens leads to activation of the transcription factor NF-κB. This contributes both to the immune response as well as survival of host cells. In infection with the bacterium Helicobacter pylori, this dual function provides a mechanistic link between the chronic inflammation and cancer development. In infection with the intracellular pathogen Legionella pneumophila, the survival oft he host cell is necessary for replication. However, in both infections, the signaling pathways leading to NF-κB activation are not well understood. Here, in order to investigate NF-κB activation, monoclonal cell lines were generated that stably express the NF-κB subunit p65 fused to GFP. Nuclear translocation of p65-GFP can be visualized by fluorescence microscopy and quantified by software-based picture analysis. Using this technology, oscillations of p65-GFP nuclear translocation could be shown after H. pylori infection. To identify new factors important for NF-κB activation, the new assay was used to conduct an RNAi-based screen. In the screen, infection with H. pylori was compared to induction with the cytokines TNFα and IL-1β. In total, 24 key regulators for NF-κB were identified. The identification of these factors broadens our understanding of NF-κB signaling and could provide targets for future therapies. Finally, detailed observation of NF-κB activation induced by L. pneumophila in single cells revealed a unique, biphasic NF-κB activation. During the first hours, bacterial flagellin induced strong but transient activation. Then, p65 translocated continuously to the nucleus over hours without oscillation. Testing an array of bacterial mutants, a tight link between bacterial replication and continuous NF-κB activation could be shown. Because this continuous nuclear localization is very unusual for a transcription factor of the NF-κB family, this indicates that L. pneumophila could manipulate NF-κB to ensure host cell survival.