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Strategic plan for CreamásOliveros Lijap, Luis Alfredo, Ponce de León Jara, Gonzalo Marcelo 10 November 2017 (has links)
The purpose of this research is to develop a 2015-2020 Strategic plan for Creamás, a
Peruvian NGO that fosters the dreams and ambition of children through education and
diverse workshops. The company began operating in the country in 2009 and throughout the
years has managed to become a referent in the NGO national industry. In order to do that the
model proposed by Fernando D’Alessio was followed throughout the nine chapters of the
document.
After thorough research, meetings with the directorates and volunteers, and on-site
visitation, data was analyzed in an attempt to decipher the current holistic situation of the
organization; its strengths, weaknesses and which opportunities and threats the external
situation posed the organization. By complementing such information with a newly proposed
vision and mission, and the identified organization’s interests; six long term objectives were
defined with their correspondent short-term objectives. Besides that, five strategies were
chosen in order for the Creamás to achieve its objectives and as a consequence, its desired
vision. Furthermore, in order for Creamás to assess the implementation of this Plan, the
document includes the proposed Balanced Scorecard; with the short-term objectives and their
correspondent indicators.
The fallouts of this work indicate that currently Creamás lacks operational efficiency
due to the poor training of its volunteers, is still unable to measure its actual impact on the
students, and does not possess a solid funding structure; all of them a must if the firm is to
remain relevant in the industry / El objetivo de este trabajo es desarrollar un Plan Estratégico de Creamás para el
periodo 2015-2020. Creamás es una ONG peruana que promueve que más niños tengan
sueños y ambiciones por medio de clases de matemática y talleres complementarios. La
empresa empezó a operar en el país en 2009 y a través de los años ha logrado convertirse en
una reconocida organización. Para el desarrollo del trabajo se siguieron los nueve pasos del
planeamiento estratégico definidos por Fernando D’Alessio (D’Alessio, 2013).
Luego de una detallada investigación, reuniones con Directores y voluntarios, y
visitas en campo, se analizó la data recogida a fin de descifrar la situación actual de la
organización; sus fortalezas, debilidades y las oportunidades y amenazas que el entorno le
presenta. Al complementar esta información con una nueva propuesta de visión y misión,
además de los intereses de la empresa; se definieron seis objetivos a largo plazo. Asimismo,
se eligieron cinco estrategias para alcanzarlos y que Creamás consiga lograr su situación
futura deseada. Para la implementación del presente Plan, el documento incluye un Tablero
de Control Balanceado con objetivos a corto plazo y sus indicadores de medición
correspondientes.
Los resultados de la investigación indican que Creamás presenta problemas en cuanto
a eficiencia operacional debido al pobre entrenamiento que reciben sus voluntarios, no es
capáz de medir el impacto que causa en los estudiantes y no cuenta aún con una sólida
estructura de donaciones; situaciones que deben ser resueltas para mantenerse relevante en la
industria.
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The ongoing coherence and otherness of God's works of creation, reconciliation, renewal and consummation of human beings and the cosmic universeHearn, Louisa Jacoba 30 November 2005 (has links)
In this dissertation we explore the concept that the works of God continue in a creative and coherent continuum from the portrayal in Genesis 1 to the description of their consummation in Revelation through the dynamic broad movement of the Kingdom of God that moved through Israel and the other nations of the world and through the churches and other institutions as well as in the Bible, in the experiences of humanity and in the vast non-human creation. Therefore creation, redemption, renewal and consummation are seen to be linked in a process which is not confined to the usual theological pointers of the Church and the Bible. Churches and the Bible are signs and instruments of the Kingdom of God amongst and in the many universes and not the only signs and instruments of the Kingdom of God in and amongst the many universes.
The importance of the coherence between each work of God, the otherness of each work and the ongoing character of God's works demonstrates itself in the impact of faith on human experience and on the very existence of human beings. The concept of developing a wholesome person with a fivesome awareness is developed, this being an awareness of a human beings creatureliness, an awareness of their sinful tendencies of doing damage to God, the self as being created by God and all other creatures of God, an awareness of the salvific and reconciliatory power of the cross and resurrection of Jesus Christ and an awareness of the renovating and renewal power of the Spirit of Pentecost which is carrying and guiding the process of the fivesome awareness in our minds, our experiences and our living into afterlife towards the consummation of all things in the new heaven and the new earth. The ongoing experience of anticipatory fragments and moments of meaning and significance breaking in from the future into our current life, bridges God's Kingdom works in the future through reflective understanding of faith experience with our everyday experience as human beings.
The basic point and the thrust of this dissertation is that of a `both...and' and not an `either...or' relationship between human beings and their experience of God as Creator, their own falling into sin, God as Reconciliator in Christ, God the Holy Spirit as the Renewer and Renovator of all things unto afterlife and the creation of the new heaven and the new earth - the whole ongoing process of God's works is attested in the Biblical texts and attested in the foursome and inclusive experiential awareness of God's works in our daily experience by the Holy Spirit. / Systematic Theology and Theological Ethics / M.Th. (Systematic Theology)
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The concept of sin in the theologies of Ellen G White and Leonardo Boff : a comparative studyZvandasara, Nkosiyabo, 1961- 03 1900 (has links)
The aim of this thesis is to compare the concept of sin in the theologies of
Ellen G. White and Leonardo Boff. Chapter 1 examines Ellen G. White's concept
of sin. White's historical and theological backgrounds coupled with her use of the
"great controversy" motif provide a better grasp of her understanding of sin. White
defines sin as the transgression of God's Law. She views sin to comprise at least
two dimensions, namely, the individual and the social. White regards these two
aspects of sin to have equal significance. White's detailed treatment of the
sanctuary teaching also highlights the two dimensions of sin.
In Chapter 2 Boff' s idea of sin is investigated. Boff' s historical background,
which exposed him to the poor, influenced his perception of sin. Boff's theological
background together with his familiarity with Karl Marx's social analysis prompted
Boff to define sin as the negation of God's love in a human history bedevilled by
class conflict. Boff views sin to have the individual and social dimensions. Yet, in
terms of importance, Boff believes that the social dimension of sin is more
consequential than the individual one.
In Chapter 3 White's and Boff s views on sin are compared. From this
comparison it is evident that both White and Boff recognize the bipolarity of sin.
Both seem to agree that christians should take an active role in correcting social
evils because love for God is manifested by how we relate to our neighbor. Boff
devotes less space to the individual aspect of sin than White.
Chapter 4 shows that White's theological tradition has a lot to learn from
Boff and his tradition and also vice versa. An awareness of the current priestly
ministry of Christ evident in White's theology could help Boff to bring some
balance to his stance on the social and the individual dimension of sin. Boff' s use
of Marx's social analysis should also help Seventh-day Adventists, the inheritors of
White's theology, not to interpret White's theology of sin only along individualistic
lines while overlooking its social dimension. / Philosophy, Practical & Systematic Theology / D.Th. (Systematic Theology)
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Überbrückung der Kluft zwischen normativer Ethik und dem Grundprinzip der Gnade mit Bezugnahme auf Scheidung und Wiederheirat innerhalb der christlichen Kirchen und Gemeinschaften / Bridging the gap between normative ethics and the concept of grace with special reference to the question of divorce and remarriage within Christian churches and communitiesBinder-Wüstiner, Beatrice, 1958- 05 1900 (has links)
German text / Die vorliegende Arbeit schlägt eine Brücke über den Graben zwischen dem normativ-ethischen
Anspruch an die Lebenslänglichkeit einer Ehe und Gottes gnädigem Handeln gegenüber dem
scheiternden Menschen. Anhand des Umgangs der Kirchen und Glaubensgemeinschaften mit
Ehescheidung und Wiederheirat wird dieser Graben aufgezeigt. Es ergibt sich, dass die bestehende
Spannung jeweils durch die einseitige Betonung von Norm oder Gnade aufgelöst wird.
Die Grundlage für die Diskussion bilden das massgebende Eheverständnis sowie die Bestimmung
des Verhältnisses zwischen theologischer Ethik und dem Konzept der Gnade als Handlungsprinzip
Gottes. Darum werden in dieser Forschungsarbeit zuerst die theologiegeschichtlich
gewachsenen Eheverständnisse und die unterschiedlichen Normen- und Gnadenverständnisse
der römisch katholischen Kirche, der evangelischen Kirchen und der evangelikalen Gemeinschaften
in Bezug auf den Umgang mit Scheidung und Wiederheirat untersucht. Aufgrund
der herausgearbeiteten Übereinstimmungen und Unterschiede wird anschliessend anhand eines
von der Vergebung ausgehenden Denkansatzes der Graben zwischen normativer Ethik und dem
Konzept der Gnade überbrückt, die Spannung aber nicht aufgehoben. Daraus werden mögliche
Auswirkungen im Umgang mit Scheidung und Wiederheirat für die Kirchen und Gemeinschaften
abgeleitet. / The present thesis bridges the gap between the normative-ethical standard of lifelong marriage
on the one hand and God’s grace for men‘s failures on the other hand. Considering the practices
of churches and communities with regard to divorce and remarriage, this gap is demonstrated.
It is found that the associated tension is eliminated by an unilateral choice of either the
normative aspect or the principle of grace. The basis for the discussion is provided by the relevant
understanding of marriage and the determination of the relationship between theological
ethics and grace as the principle of God's action towards mankind. Therefore, this thesis investigates
the evolution of the theological understanding of marriage during history and the different
understandings of norms and grace in the Catholic Church, the Protestant Church and the
Evangelical Free Communities with regard to their handling of divorce and remarriage. Considering
the resulting similarities and differences, I propose – building on the fact of God’s forgiveness
– how to bridge the gap between normative ethics and the concept of grace without
eliminating the obvious tension. Finally, possible consequences for how to handle divorce and
remarriage by churches and communities are drawn. / Philosophy & Systematic Theology / M. Th. (Theological Ethics)
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Construção e análise funcional de vetores lentivirais de interesse biotecnológico / Construction and functional analysis of lentiviral vectors for biotechnological purposesVedoveli, Naiara Cristina Pulzi Saito 16 May 2016 (has links)
Vetores lentivirais são ferramentas fundamentais para modificação celular. Sua utilização ganhou destaque devido à capacidade desses em integrar ao genoma de células que estão ou não em divisão. Grande parte dos vetores desenvolvidos são derivados do genoma do Vírus da Imunodeficiência Humana (HIV-1), portanto, modificações foram necessárias a fim de evitar a formação de Partículas Competentes em Replicação (RCLs) e garantir uma utilização segura. Com as modificações, foram produzidos os vetores lentivirais de terceira geração utilizados atualmente. Esses vetores podem ser usados para expressão constitutiva de genes, produção de proteínas recombinantes, produção de animais transgênicos e terapia gênica. Com isso, torna-se necessário o desenvolvimento de vetores lentivirais para aplicação em pesquisa básica e ensaios clínicos. Dessa forma, o presente estudo teve por objetivo a construção de vetores de expressão lentivirais aplicáveis à: 1- expressão constitutiva de genes de interesse e 2-vetores com promotores específicos para expressão de proteínas em megacariócitos. Esse trabalho descreve a construção desses vetores, sua importância e discute suas possíveis aplicações. As sequências selecionadas para produção dos vetores foram: os genes Runx1C e VkorC1 e os promotores proPF4 e proITGA2b. Todas as sequências encontram-se clonadas em vetor de clonagem e estoques de bactérias com esses vetores congeladas em glicerol foram confeccionados. Para a confecção dos vetores lentivirais, o gene Runx1C foi subclonado no vetor lentiviral base p1054-CIGWS sob controle do promotor forte CMV, enquanto o promotor proITGA2b foi subclonado no vetor base p1054-FVIII, em substituição ao promotor CMV, de forma a controlar a expressão de FVIII. Os dois vetores produzidos apresentam ainda o gene para proteína verde GFP precedida do sítio de ligação do ribossomo IRES, com expressão controlada pelo mesmo promotor interno do vetor. O trabalho possibilitou, portanto, a produção de dois vetores lentivirais bi-cistrônicos: p1054-Runx1C e pL-proITGA2b-FVIII. A construção p1054-Runx1C ainda não foi sequenciada, mas foi confirmada por restrição enzimática e apresenta potencial para aplicação em estudos de diferenciação hematopoética. Já a construção pL-proITGA2b-FVIII foi sequenciada, porém sem confirmação da região de ligação do proITGA2b ao vetor. Reações de PCR e de restrição enzimática confirmaram a ligação e sequenciamento mostrou 67% de similaridade entre a região sequenciada e o promotor ITGA2b depositado no banco de dados. Análise funcional foi realizada através da transfecção desse vetor em células HEK-293T. As células transfectadas apresentaram expressão positiva para GFP e secreção de FVIII no sobrenadante celular, evidenciando que o promotor proITGA2b clonado no vetor encontra-se ativo. Esse vetor apresenta potencial para aplicação em terapia gênica para hemofilias, pois apresenta expressão do fator de coagulação direcionado a megacariócitos e plaquetas, células que estão diretamente relacionadas ao processo de coagulação, representando grandes veículos para secreção desses fatores. Ainda, os dois vetores lentivirais gerados apresentam segurança e eficiência elevadas, pois são vetores de terceira geração auto-inativantes (SIN) e apresentam elementos regulatórios que melhoram o transporte e integração do DNA ao genoma hospedeiro. / Lentiviral vectors are fundamental tools for cell modification that gained prominence due to their ability to integrate the genome of non-dividing cells. Most of developed lentiviral vectors are derived from the genome of Human Immunodeficiency Virus (HIV-1), so modifications were necessary in order to avoid the formation of Competent Replication Particles (RCLs) and ensure safer operations. The modifications led to development of third generation lentiviral vectors currently used. These vectors can be used for constitutive gene expression, production of recombinant protein, production of transgenic animals and gene therapy. It\'s evident the need to develop lentiviral vectors for application in basic research and clinical trials. Thus this study aimed to construct lentiviral expression vectors applicable to: 1- constitutive expression of genes of interest and 2-vectors with specific promoters for expression of proteins in megakaryocytes and platelets. This paper describes the construction of these vectors, their importance and discuss their possible applications. Sequences were selected for production of the vectors: genes Runx1C and VkorC1 and proPF4 and proITGA2b promoters. All four sequences are cloned into cloning vectors and stocks of bacteria with these vectors frozen in glycerol were prepared. Lentiviral vectors were engineered from subcloning the sequence Runx1C into the basic lentiviral vector p1054- CIGWS under control of the strong CMV promoter, and from subcloning proITGA2b promoter into p1054-FVIII basic vector, replacing the CMV promoter in order to control the expression of FVIII. Both vectors exhibit the green fluorescence protein GFP gene preceded by a ribosome binding site IRES under control of vector\'s internal promoter. Therefore, this work resulted in the production of two bi-cistronic lentiviral vectors: p1054-Runx1C and pLproITGA2b-FVIII. The p1054-Runx1C construction has not yet been sequenced, but it was confirmed by digestion and has potential for use in hematopoietic differentiation studies. Though, pL-proITGA2b-FVIII construct was sequenced, but the technique didn\'t allow to confirm the binding region between proITGA2b and the vector. Although PCR reaction and digestion confirmed the construction. Sequence analysis showed 67% similarity between the sequenced region and ITGA2b promoter deposited in the database. Functional analysis was performed by transfection of this vector in HEK-293T cells. The transfected cells showed positive expression of GFP and FVIII secretion in cell supernatant, indicating that the proITGA2b promoter cloned into the vector is active. This vector has potential usage in gene therapy for hemophilia, since it can be used to express coagulation factors in megakaryocytes and platelets and these cells are directly related to the clotting process, representing great vehicles for secretion of these factors. Even more, the two lentiviral vectors generated have higher safety and efficiency, as they are self-inactivating (SIN) third-generation vectors and have regulatory elements that enhance transport and integration of DNA into the host genome.
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Três poetas e três tempos do exílio espanhol de 1939: Luis Cernuda, Emilio Prados e Max Aub / Three poets and three periods of the 1939 Spanish exile: Luis Cernuda, Emilio Prados and Max AubForneron, Ivan Martucci 10 November 2015 (has links)
Este trabalho se debruça sobre a poesia do exílio republicano espanhol de 1939. A pesquisa procura demonstrar que o tempo de duração do exílio é fator preponderante para a leitura de uma produção poética ampla e dispersa. Desse modo, seu enfoque central trata de investigar como essa poética exilada assimilou e ressignificou as três décadas de exílio, reconstruindo a identidade de seus autores. O corpus é composto por livros de inflexão da obra de três autores: Luis Cernuda (Vivir sin estar viviendo, 1944-1949), Emilio Prados (La piedra escrita, 1959-1961) e Max Aub (Antología traducida, 1963-1971). / The present study examines the 1939 Spanish Republican exile poetry. The investigation aims to demonstrate that the time the exile lasted is a fundamental aspect when reading such a widespread poetic production. Thus, the focus is on analyzing in which way this exiled poetry incorporated and resignified the three decades of exile, reconstructing the authors identity. Critical books in three authors works compose the corpus: Luis Cernuda (Vivir sin estar viviendo, 1944-1949), Emilio Prados (La piedra escrita, 1959- 1961) and Max Aub (Antología traducida, 1963-1971).
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Transmission electron microscopy investigation of growth and strain relaxation mechanisms in GaN (0001) films grown on silicon (111) substratesMarkurt, Toni 08 January 2016 (has links)
In dieser Arbeit untersuchen wir die grundlegenden Wachstums- und Relaxationsprozesse, die es erlauben den Verzerrungszustand von GaN (0001) beim Wachstum auf Silizium (111) Substraten einzustellen und die resultierende Dichte an Durchstoßversetzungen zu reduzieren. Zu deren Analyse werden GaN (0001) Schichten, die mittels metallorganischer Gasphasenepitaxy abgeschieden worden sind, hauptsächlich mit transmissionselekronenmikroskopischen Methoden untersucht. Die wesentlichen Erkenntnisse der Arbeit sind: (i) Der Aufbau einer kompressiven Verzerrung von GaN (0001) Filmen mittels AlGaN Zwischenschichten beruht auf einer Asymmetrie der plastischen Relaxation an den beiden Grenzflächen der AlGaN Zwischenschicht. Fehlpassungsversetzungen bilden sich zwar an beiden Grenzflächen aus, jedoch ist der mittlere Abstand zwischen Versetzungslinien an der unteren Grenzfläche kleiner, als an der oberen. (ii) Plastische Relaxation von verzerrten (0001) Wurtzit Schichten erfolgt im Wesentlichen durch Bildung von a-Typ Fehlpassungsversetzungen im 1/3 |{0001} Gleitsystem. Diese bilden sich aber nur dann, wenn die verzerrten Schichten eine 3-D Morphologie aufweisen. Eine quantitative Modellierung dieses Prozesses zeigt, dass die kritische Schichtdicke für das Einsetzen der plastischen Relaxation wesentlich vom Wachstumsmodus bestimmt wird. (iii) Eine Silizium Delta-Dotierung der GaN (0001) Oberfläche führt zum Wachstum einer kohärenten Sub-Monolage SiGaN3, die eine periodisch Anordnung von Silizium- und Galliumatomen, sowie Galliumvakanzen aufweist. Da das Wachstum von GaN direkt auf der SiGaN3-Monolage unterdrückt ist, tritt ein Übergang zu 3-D Inselwachstum auf, das zunächst ausschließlich in Löchern der SiGaN3-Monolage anfängt. Eine hohe Konzentration von Silizium auf der GaN (0001) Oberfläche wirkt also als Anti-Surfactant beim epitaktischen Wachstum von GaN. Rechnungen mittels der Dichtefunktionaltheorie liefern Erklärungen für das beobachtete Wachstumsverhalten. / In this work we study the basic growth and relaxation processes that are used for strain and dislocation engineering in the growth of GaN (0001) films on silicon (111) substrates. To analyse these processes, samples, grown by metalorganic vapour phase epitaxy were investigate by means of transmission electron microscopy. Our investigations have revealed the following main results: (i) Strain engineering and build-up of compressive strain in GaN (0001) films by means of AlGaN interlayer is based on an asymmetry in plastic relaxation between the two interfaces of the AlGaN interlayer. Although misfit dislocation networks form at both interfaces of the interlayer, the average spacing of dislocation lines at the lower interface is smaller than that at the upper one. (ii) Plastic relaxation of strained (0001) wurtzite films is caused mainly by formation of a-type misfit dislocations in the 1/3 |{0001} slip-system. These a-type misfit dislocations form once the strained films undergo a transition to a 3-D surface morphology, e.g. by island growth or cracking. Quantitative modelling of this process reveals that the critical thickness for nucleation of a-type misfit dislocations depends next to the lattice mismatch mainly on the growth mode of the film. (iii) Silicon delta-doping of the GaN (0001) surface leads to the growth of a coherent sub-monolayer of SiGaN3 that shows a periodic arrangement of silicon and gallium atoms and gallium vacancies. Since growth of thick GaN layers directly on top of the SiGaN3-monolayer is inhibited a transition towards 3-D island growth occurs, whereby GaN islands exclusively nucleate at openings in the SiGaN3-monolayer. A high concentration of silicon on the GaN (0001) surface thus acts as an anti-surfactant in the epitaxial growth of GaN. Our density functional theory calculations provide an explanation for both the self-limited growth of the SiGaN3-monolayer, as well as for the blocking of GaN growth on top of the SiGaN3-monolayer.
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Construção e análise funcional de vetores lentivirais de interesse biotecnológico / Construction and functional analysis of lentiviral vectors for biotechnological purposesNaiara Cristina Pulzi Saito Vedoveli 16 May 2016 (has links)
Vetores lentivirais são ferramentas fundamentais para modificação celular. Sua utilização ganhou destaque devido à capacidade desses em integrar ao genoma de células que estão ou não em divisão. Grande parte dos vetores desenvolvidos são derivados do genoma do Vírus da Imunodeficiência Humana (HIV-1), portanto, modificações foram necessárias a fim de evitar a formação de Partículas Competentes em Replicação (RCLs) e garantir uma utilização segura. Com as modificações, foram produzidos os vetores lentivirais de terceira geração utilizados atualmente. Esses vetores podem ser usados para expressão constitutiva de genes, produção de proteínas recombinantes, produção de animais transgênicos e terapia gênica. Com isso, torna-se necessário o desenvolvimento de vetores lentivirais para aplicação em pesquisa básica e ensaios clínicos. Dessa forma, o presente estudo teve por objetivo a construção de vetores de expressão lentivirais aplicáveis à: 1- expressão constitutiva de genes de interesse e 2-vetores com promotores específicos para expressão de proteínas em megacariócitos. Esse trabalho descreve a construção desses vetores, sua importância e discute suas possíveis aplicações. As sequências selecionadas para produção dos vetores foram: os genes Runx1C e VkorC1 e os promotores proPF4 e proITGA2b. Todas as sequências encontram-se clonadas em vetor de clonagem e estoques de bactérias com esses vetores congeladas em glicerol foram confeccionados. Para a confecção dos vetores lentivirais, o gene Runx1C foi subclonado no vetor lentiviral base p1054-CIGWS sob controle do promotor forte CMV, enquanto o promotor proITGA2b foi subclonado no vetor base p1054-FVIII, em substituição ao promotor CMV, de forma a controlar a expressão de FVIII. Os dois vetores produzidos apresentam ainda o gene para proteína verde GFP precedida do sítio de ligação do ribossomo IRES, com expressão controlada pelo mesmo promotor interno do vetor. O trabalho possibilitou, portanto, a produção de dois vetores lentivirais bi-cistrônicos: p1054-Runx1C e pL-proITGA2b-FVIII. A construção p1054-Runx1C ainda não foi sequenciada, mas foi confirmada por restrição enzimática e apresenta potencial para aplicação em estudos de diferenciação hematopoética. Já a construção pL-proITGA2b-FVIII foi sequenciada, porém sem confirmação da região de ligação do proITGA2b ao vetor. Reações de PCR e de restrição enzimática confirmaram a ligação e sequenciamento mostrou 67% de similaridade entre a região sequenciada e o promotor ITGA2b depositado no banco de dados. Análise funcional foi realizada através da transfecção desse vetor em células HEK-293T. As células transfectadas apresentaram expressão positiva para GFP e secreção de FVIII no sobrenadante celular, evidenciando que o promotor proITGA2b clonado no vetor encontra-se ativo. Esse vetor apresenta potencial para aplicação em terapia gênica para hemofilias, pois apresenta expressão do fator de coagulação direcionado a megacariócitos e plaquetas, células que estão diretamente relacionadas ao processo de coagulação, representando grandes veículos para secreção desses fatores. Ainda, os dois vetores lentivirais gerados apresentam segurança e eficiência elevadas, pois são vetores de terceira geração auto-inativantes (SIN) e apresentam elementos regulatórios que melhoram o transporte e integração do DNA ao genoma hospedeiro. / Lentiviral vectors are fundamental tools for cell modification that gained prominence due to their ability to integrate the genome of non-dividing cells. Most of developed lentiviral vectors are derived from the genome of Human Immunodeficiency Virus (HIV-1), so modifications were necessary in order to avoid the formation of Competent Replication Particles (RCLs) and ensure safer operations. The modifications led to development of third generation lentiviral vectors currently used. These vectors can be used for constitutive gene expression, production of recombinant protein, production of transgenic animals and gene therapy. It\'s evident the need to develop lentiviral vectors for application in basic research and clinical trials. Thus this study aimed to construct lentiviral expression vectors applicable to: 1- constitutive expression of genes of interest and 2-vectors with specific promoters for expression of proteins in megakaryocytes and platelets. This paper describes the construction of these vectors, their importance and discuss their possible applications. Sequences were selected for production of the vectors: genes Runx1C and VkorC1 and proPF4 and proITGA2b promoters. All four sequences are cloned into cloning vectors and stocks of bacteria with these vectors frozen in glycerol were prepared. Lentiviral vectors were engineered from subcloning the sequence Runx1C into the basic lentiviral vector p1054- CIGWS under control of the strong CMV promoter, and from subcloning proITGA2b promoter into p1054-FVIII basic vector, replacing the CMV promoter in order to control the expression of FVIII. Both vectors exhibit the green fluorescence protein GFP gene preceded by a ribosome binding site IRES under control of vector\'s internal promoter. Therefore, this work resulted in the production of two bi-cistronic lentiviral vectors: p1054-Runx1C and pLproITGA2b-FVIII. The p1054-Runx1C construction has not yet been sequenced, but it was confirmed by digestion and has potential for use in hematopoietic differentiation studies. Though, pL-proITGA2b-FVIII construct was sequenced, but the technique didn\'t allow to confirm the binding region between proITGA2b and the vector. Although PCR reaction and digestion confirmed the construction. Sequence analysis showed 67% similarity between the sequenced region and ITGA2b promoter deposited in the database. Functional analysis was performed by transfection of this vector in HEK-293T cells. The transfected cells showed positive expression of GFP and FVIII secretion in cell supernatant, indicating that the proITGA2b promoter cloned into the vector is active. This vector has potential usage in gene therapy for hemophilia, since it can be used to express coagulation factors in megakaryocytes and platelets and these cells are directly related to the clotting process, representing great vehicles for secretion of these factors. Even more, the two lentiviral vectors generated have higher safety and efficiency, as they are self-inactivating (SIN) third-generation vectors and have regulatory elements that enhance transport and integration of DNA into the host genome.
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El "homeschooling" en España: descripción y análisis del fenómenoCabo González, Carlos 27 April 2012 (has links)
Descripción y análisis del fenómeno del homeschooling en España desde la doble perspectiva sincrónica (circunscrita al año 2009) y diacrónica (desde sus comienzos hasta nuestros días). Esta descripción y análisis se estructura en cuatro grandes apartados o capítulos.
En el primero de ellos, se fijan los objetivos del trabajo; se establece la metodología; se delimitan los conceptos más frecuentemente utilizados en el estudio; se define el fenómeno; se informa de las diferentes denominaciones con las que se conoce esta práctica; y, finalmente se ofrece un panorama de la situación en la que se encuentra el homeschooling en el mundo (países en los que está oficialmente reconocido).
En el segundo capítulo, se realiza una descripción histórica de los principales acontecimientos del homeschooling español, desde sus comienzos hasta 2012.
En el tercer capítulo se ofrecen los resultados obtenidos en las dos encuestas realizadas sobre el homeschooling en España.
En el cuarto y último capítulo, se aborda el homeschooling español desde una perspectiva cualitativa.
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The ongoing coherence and otherness of God's works of creation, reconciliation, renewal and consummation of human beings and the cosmic universeHearn, Louisa Jacoba 30 November 2005 (has links)
In this dissertation we explore the concept that the works of God continue in a creative and coherent continuum from the portrayal in Genesis 1 to the description of their consummation in Revelation through the dynamic broad movement of the Kingdom of God that moved through Israel and the other nations of the world and through the churches and other institutions as well as in the Bible, in the experiences of humanity and in the vast non-human creation. Therefore creation, redemption, renewal and consummation are seen to be linked in a process which is not confined to the usual theological pointers of the Church and the Bible. Churches and the Bible are signs and instruments of the Kingdom of God amongst and in the many universes and not the only signs and instruments of the Kingdom of God in and amongst the many universes.
The importance of the coherence between each work of God, the otherness of each work and the ongoing character of God's works demonstrates itself in the impact of faith on human experience and on the very existence of human beings. The concept of developing a wholesome person with a fivesome awareness is developed, this being an awareness of a human beings creatureliness, an awareness of their sinful tendencies of doing damage to God, the self as being created by God and all other creatures of God, an awareness of the salvific and reconciliatory power of the cross and resurrection of Jesus Christ and an awareness of the renovating and renewal power of the Spirit of Pentecost which is carrying and guiding the process of the fivesome awareness in our minds, our experiences and our living into afterlife towards the consummation of all things in the new heaven and the new earth. The ongoing experience of anticipatory fragments and moments of meaning and significance breaking in from the future into our current life, bridges God's Kingdom works in the future through reflective understanding of faith experience with our everyday experience as human beings.
The basic point and the thrust of this dissertation is that of a `both...and' and not an `either...or' relationship between human beings and their experience of God as Creator, their own falling into sin, God as Reconciliator in Christ, God the Holy Spirit as the Renewer and Renovator of all things unto afterlife and the creation of the new heaven and the new earth - the whole ongoing process of God's works is attested in the Biblical texts and attested in the foursome and inclusive experiential awareness of God's works in our daily experience by the Holy Spirit. / Systematic Theology and Theological Ethics / M.Th. (Systematic Theology)
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