Realizaram-se campanhas em árvores de cinco anos de idade de Pinus caribaea var. hondurensis e Pinus taeda em parcelas controle (sem fertilização e sem irrigação) e parcelas fertilizadas e irrigadas, durante o verão e o inverno de 2012 visando caracterizar as seguintes variáveis fisiológicas: i) Capacidade máxima fotossintética (Amax); ii) Fotossíntese ao longo do dia (A); iii) Variação da condutância estomática (gs) em relação ao aumento do déficit de pressão de vapor (DPV); e iv) Taxas máximas de carboxilização (Vcmax) e de transporte de elétrons (Jmax) via curvas A/Ci. O estudo foi realizado no projeto Produtividade Potencial do Pinus no Brasil, localizado na Estação Experimental da ESALQ/USP em Itatinga-SP.Foram escolhidas três árvores médias por parcela para as avaliações fisiológicas, realizadas com o LiCor 6400XT. A mensuração da Amax foi realizada no terço médio da copa, em dois galhos por árvore e em duas posições por galho, sendo realizada das 8 às 10 horas, e o comportamento da A, gs com o aumento do DPV, ocorreram de hora em hora, das 11 às 15 horas. Ao final, as acículas foram coletadas para a determinação da área foliar específica (AFE) e do nitrogênio foliar. As curvas A/Ci foram realizadas nas três árvores, um galho por árvore e duas posições por galho, entre 8 e 12 horas. Aos cinco anos o Pinus caribaea var. hondurensis apresenta o dobro do volume de madeira do que o Pinus taeda. As duas avaliações fisiológicas mostraram valores similares entre tratamentos, para cada espécie. Os valores de Amax foram maiores durante o verão e o Pinus caribaea var. hondurensis mostrou grande sensibilidade, comparativamente ao Pinus taeda. Ao analisar os dados de A e gs ao longo do dia, observa-se também maiores variações do Pinus caribaea var. honduresis. Os valores médios de Amax para o verão e o inverno foram 8,2 e 4,8 ?mol m-2 s-1 e 6,8 e 6,3 ?mol m-2 s-1 para o Pinus caribaea var. hondurensis e o Pinus taeda, respectivamente. Ocorreu redução dos valores de A e gs com o aumento do DPV, para ambas as campanhas em relação ao Pinus caribaea var. hondurensis e somente no inverno para o Pinus taeda. As duas espécies apresentaram relação positiva entre fotossíntese e transpiração, sendo que o Pinus caribaea var. hondurensis apresenta maior eficiência no uso da água. As médias da AFE e nitrogênio foliar foram de 9,6 m²kg-1, 10,1g Kg-1 e 10,0 m²kg-1, 13,4g Kg-1, para o Pinus caribaea var. hondurensis e Pinus taeda, respectivamente. Em relação aos parâmetros fotossintéticos o Pinus taeda se destacou em ambas as campanhas, com valores médios de Vcmax e Jmax maiores que o Pinus caribaea var. hondurensis, relacionado à maiores concentrações de nitrogênio foliar. Não houve relação entre o crescimento em biomassa das árvores e as medições da fotossíntese a nível foliar, indicando que outros processos a nível de copa, uso e alocação de fotossintetizados devem ser investigados para explicar a diferença de crescimento. / The campaigns were conducted in trees with five years old of Pinus caribaea var. hondurensis and Pinus taeda in control plots (no fertilization and no irrigation) and fertilized and irrigated plots during summer and winter of 2012 to characterize the physiological variables: i) maximum photosynthetic capacity (Amax), ii) Photosynthesis throughout the day (A); iii) Changes in stomatal conductance (gs) in relation to the increase in vapor pressure deficit (VPD), and iv) Maximum rates of carboxilization (Vcmax) and maximum rates of electron transport (Jmax) based on A/Ci curves. The study was conducted in the project Potential Productivity of Pinus in Brazil, located at the Experimental Station of ESALQ/USP in Itatinga-SP. Three average trees per plot were chosen for physiological evaluations, performed with the LiCor 6400XT. The Amax measurement was performed in the middle third of the crown, in two branches per tree and two positions per branch, taken from 8 to 10am. To get the response of A and gs with increasing VPD, the measurements continued every hour, from 11 am to 3 pm. At the end of the measurements, the needles were collected for determination of specific leaf area (SLA) and leaf nitrogen (N). The A/Ci curves were performed in three trees, one branch per tree and two positions per branch were taken from 8 am to 12 pm. At five years, the Pinus caribaea var. hondurensis showed two-fold the wood volume of Pinus taeda. Both physiological measurements showed similar results between treatments for each species. Amax values were higher during summer, and Pinus caribaea var. hondurensis shower greater sensitivity compared to Pinus taeda. A and gs throughout the day showed higher variation in Pinus caribaea var. hondurensis. The average values of Amax for summer and winter were 8.2, 4.8 ?mol m-2 s-1 and 6.75, 6.3 ?mol m-2 s-1 for Pinus caribaea var. hondurensis and Pinus taeda, respectively. There was a reduction of A and gs with the increasing of DPV, for both campaigns for the Pinus caribaea var. hondurensis and only in winter campaign for Pinus taeda. Thus, the two species have different behaviors in response to climatic changes. The two species showed a positive relationship between photosynthesis and transpiration, with Pinus caribaea var. hondurensis showing greater water use efficiency. The average SLA and needle nitrogen were 9.6 m² kg-1, 10.1g kg-1 and 10 m² kg-1, 13.4g kg-1 for Pinus caribaea var. hondurensis and Pinus taeda, respectively. Photosynthetic parameters in Pinus taeda was higher in both campaigns, with average values of Vcmax and Jmax greater than in Pinus caribaea var. hondurensis, related to higher concentration of needle nitrogen. There was no relationship between tree biomass growth and leaf-level measurements of photosynthesis, indicating that other processes at crown level, use and allocation of photosynthates should be investigated to explain the difference in growth.

Conhecer o uso da água pelas árvores é fundamental para entender as interações entre o ambiente e as florestas e compreender seu crescimento por meio do entendimento dos processos que o governa. Objetivou-se com o trabalho determinar as taxas de transpiração, o crescimento e a eficiência do uso da água de árvores de Eucalyptus, por meio do método de Granier, em parcelas com e sem irrigação, além de compará-lo ao modelo de Penman-Monteith para estimativa da transpiração. O experimento foi realizado em um plantio de clone de Eucalyptus, instalado em março de 2001, no município de Eunápolis, BA. Para determinação da transpiração, foi avaliado o fluxo xilemático no período de agosto a dezembro de 2005, por meio de sondas instaladas no tronco das árvores. Para avaliação do crescimento das plantas, estimouse o incremento de biomassa nos tratamentos a cada quatro meses, enquanto que a eficiência do uso da água (EUA) foi obtida pela razão entre o incremento de biomassa e quantidade de água transpirada no período. Adicionalmente, foi avaliada a variação da umidade no perfil do solo pelo sistema TDR nos dois tratamentos. Foi necessário calibrar o método de Granier, pois se observou que a equação original subestimou significativamente o uso de água. A densidade mensal do fluxo de seiva variou de 16,4 a 35,5 cm³ cm-2 hora-1, sem diferir entre os tratamentos. Enquanto a transpiração média das árvores irrigadas foi igual ou superior às não irrigadas, atingindo valores da ordem de 68 a 79 L arv-1 dia-1, o incremento de biomassa foi superior no tratamento irrigado (16 kg planta-1) em relação ao não irrigado (10 kg planta-1), ou seja, o eucalipto não apresentou consumo de luxo de água, pois a água a mais transpirada pela árvores irrigadas foi efetivamente usada para auxiliar a fixação de carbono no tronco. A EUA não diferiu entre os tratamentos, com média de 1,0 g L-1. Observou-se que, independentemente do tratamento, árvores com maior biomassa inicial apresentaram EUA superior às árvores de menor porte durante o período do estudo. Obteve-se uma relação não linear entre condutância da copa e déficit de pressão de vapor, indicando que o eucalipto apresenta um controle estomático que limita a transpiração. Quando avaliado na escala mensal, o modelo de Penman-Monteith estimou com precisão a transpiração do eucalipto. / Estimating the water use by trees is fundamental to understand the interactions between environment and plants and to know more about the processes that governs it. This work aimed to determine the transpiration rates, the growth and the water use efficiency of clonal Eucalyptus trees, using the Granier method, with or without irrigation, besides comparing it to the Penman-Monteith model for estimating transpiration. The experiment was accomplished in a Eucalyptus clonal plantation installed in March of 2001, in Eunapolis city, State of Bahia, Brazil. For determination of transpiration, the sap flow was evaluated in the period of August to December of 2005, using probes installed in the trees stems. To evaluate tree growth the biomass increment was considered in the treatments every four months, while the water use efficiency (WUE) was obtained by the ratio between the biomass increment and amount of water transpired in the period. Additionally, the variation of humidity was evaluated in the soil profile by the TDR system in both treatments. It was necessary to calibrate the Granier method because it was observed that the original equation underestimated the water use. The monthly sap flow density ranged from 16,4 to 35,5 cm³ cm-2 hour-1 and not differing between treatments. While the average transpiration of the irrigated trees was equal or superior to the no irrigated ones, with values varying from 68 to 79 L day-1 per tree, the biomass increment was superior in the irrigated treatment (16 kg tree-1) in relation to the no irrigated (10 kg tree-1). This results points that the Eucalyptus did not present luxury consumption of water, because the additional water transpired by the irrigated trees was used to allocate more carbon to the stem. The WUE did not differ between treatments, with average of 1,0 g L-1. It was observed that, independently of the treatment, trees with larger initial biomass presented highest WUE in relation to the smaller trees during the studied period. It was obtained a no linear relationship between canopy conductance and vapor pressure deficit, indicating that Eucalyptus presents a strong stomatal control that limit the transpiration. When used at monthly scale, Penman-Monteith model evaluated the transpiration of the Eucalyptus accurately.

Made available in DSpace on 2014-08-20T14:36:54Z (GMT). No. of bitstreams: 1 Dissertacao_Marilia_Alves_Brito_Pinto.pdf: 1469883 bytes, checksum: 0e148abbe007d166eb1aa6a2992757ac (MD5) Previous issue date: 2011-03-04 / With the incentive of biodiesel production in Brazil, the castor bean crop has gained importance in the agricultural scenario, mainly because its seeds have high oil content of good quality. Being considered drought resistant plant in most reports of the literature, this is a controversial aspect because under water deficit conditions low yield has been recorded. Therefore this study had the objective to evaluate the growth and development of the castor bean submitted to different soil water tensions. A greenhouse experiment was carried out with plants of the cultivar Al Guarany 2002. Plants were cultivated in pots with one tensiometer installed at the 0.15 m depth to monitor the water soil tension. The experimental design was completely randomized with three treatments and four replications: 1- water tension maintained at 0.01 MPa; 2 when the soil water tension reached 0.03 MPa, water was added to until returning 0.01 MPa; 3 when the soil water tension reached 0.06 MPa, water was added to until returning to 0.01 MPa. Between 30 and 105 days after plant emergence (DAE) plant transpiration, leaf area, plant height and stem diameter were biweekly evaluated. The time lapse to onset of flowering and height of insertion of the first raceme were evaluated. Physiological parameters such as leaf water potential, leaf firmness and stomatal resistance were measured at 105 DAE. Results show that increasing soil water tension decreased the transpiration and growth rates of the castor bean plant. The time to flowering and height of insertion of the first raceme were greater for plants submitted to lower soil water tension. The leaf water potential and leaf firmness decreased as the soil water tension decreased. As soil water tension increased the stomatal resistance increased mainly with the rise of the atmosphere evaporative demand. Keywords: transpiration, water stress, growth e development. / Com o incentivo à produção de biodiesel no Brasil, a cultura da mamona ganhou importância no cenário agrícola, visto que suas sementes tem alto teor de óleo de boa qualidade. Aliada a estas características está a resistência à seca, que envolve um aspecto controverso, pois o que se tem observado são baixas produtividades em condições de déficit hídrico. Em virtude disso, este trabalho teve como objetivo avaliar a resposta da cultura da mamona a diferentes tensões de água no solo, determinando o efeito das condições de déficit hídrico no crescimento e desenvolvimento da planta. Para tal, foi desenvolvido um experimento em ambiente protegido, com plantas de mamona da cultivar Al Guarany 2002. As plantas foram cultivadas em vasos nos quais foram instalados tensiômetros a 0,15 m de profundidade para monitoramento da tensão de água no solo. O delineamento experimental foi inteiramente casualizado, com três tratamentos e quatro repetições: 1 tensão mantida em 0,01 MPa; 2 quando a tensãochegava a 0,03 MPa as plantas eram irrigadas para retorna-la a 0,01 MPa; 3 quando a tensão chegava a 0,06 MPa as plantas eram irrigadas pararetorna-la a 0,01 MPa. No intervalo entre 30 e 105 dias após a emergência (DAE) foram avaliadas quinzenalmente a transpiração, a área foliar, a altura da planta e o diâmetro caulinar. Determinou-se ainda o tempo para início da floração e altura de inserção do primeiro racemo. Aos 105 DAE foram realizadas as avaliações dos parâmetros fisiológicos, potencial de água na folha, firmeza da folha e resistência estomática. Os resultados indicam que a mamona é sensível ao aumento da tensão de água no solo, sendo que em condições de maior tensão ela diminui a transpiração e o seu crescimento. O tempo para floração e a altura de inserção do primeiro racemo foram maiores nas plantas submetidas a menores tensões de água. O potencial de água na folha e a sua firmeza diminuiram com o aumento da tensão de água. O aumento da tensão de água no substrato aumentou a resistência estomática nas folhas da mamoneira, principalmente com o aumento da demanda evaporativa da atmosfera.

Orientador: Jose Teixeira Filho / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Agricola / Made available in DSpace on 2018-08-04T06:12:33Z (GMT). No. of bitstreams: 1 TeixeiraFilho_AristotelesdeJesus_D.pdf: 3254132 bytes, checksum: aae8c8f2bd08b359e5b2d2c647e86b0e (MD5) Previous issue date: 2005 / Resumo: As variáveis ecofisiológicas exercem grande influência, não somente no comportamento vegetativo das plantas cultivadas, mas, sobretudo no seu desempenho em relação às características produtivas. Os estudos sobre trocas gasosas têm-se ampliado desde a década de oitenta, dada a sua importância em cultivos tropicais, tal qual, em variedades de citros. Tem-se investigado que os estômatos são o principal ponto de controle do fluxo de água, sendo o déficit de pressão hídrica do ar apontada como a força motora para a transpiração, e a quantidade de água no solo é o fator decisivo para o abastecimento hídrico da planta. Dessa forma, a eficiência do uso da água pela cultura pode ser considerada como uma relação custo-benefício para o aumento da produção dos citros, com o mínimo impacto na reserva hídrica do ecossistema, haja vista que no Brasil, apesar da incidência de períodos de déficits hídricos temporários em várias regiões citrícolas, predomina o cultivo sem irrigação. O Brasil é um dos maiores produtores mundiais de frutas cítricas, destacando-se os Estados: Bahia, Minas Gerais, Rio Grande do Sul, Rio de Janeiro, São Paulo e Sergipe. Com plantios acima de 1.100.000 hectares, os citros têm grande importância comercial no Brasil, sendo São Paulo o maior produtor com aproximadamente 70% da produção nacional. O retorno econômico proveniente deste produto é de grande importância para o crescimento do setor agrícola do país. A partir da safra de 1979-1981, a citricultura brasileira vem ocupando o primeiro lugar em produção dentro da América do Sul. Para tal, o estudo foi conduzido no campo experimental da Faculdade de Engenharia Agrícola (FEAGRI) da Universidade Estadual de Campinas, localizado no município de Campinas, entre as coordenadas geográficas de 22o53¿20¿ de Latitude sul e o meridiano 47o04¿40¿ de Longitude oeste de Greenwich. O experimento foi conduzido em cinco plantas de citros de mesma variedade (Citrus sinensis L. Osbeck), realizando medidas de transpiração, condutância estomática, radiação fotossinteticamente ativa, radiação incidente total e déficit de pressão de vapor saturado. No período considerado observou-se que a maior média diária da transpiração 4,63 mmol.m-2.s-1 na PL4T2 e a menor foi de 1,53 mmol.m-2.s-1 na PL1EVP1. O melhor modelo foi aquele que considerou que resposta da transpiração foliar estar diretamente associado ao déficit de pressão de vapor saturado / Abstract: The variables ecofisiológicas exercise great influence, not only in the vegetative behavior of the cultivated plants, but, above all in your acting in relation to the productive characteristics. The studies on gas exchange have been enlarging since the decade of eighty, given your importance in tropical cultivations, just as, in citrus varieties. Has been investigating that the stomatal is the principal point of control of the flow of water, being the deficit of pressure water of the air appeared as the motive force for the perspiration, and the amount of water in the soil it is the decisive factor for the provisioning water of the plant. In that way, the efficiency of the use of the water for the culture can be considered as a relationship cost-benefit for the increase of the production of the citrus, with the minimum impact in the reservation water of the ecosystem, have seen that in Brazil, in spite of the incidence of periods of deficits temporary waters in several areas citrícolas, the cultivation prevails without irrigation. Brazil is one of the largest world producers of citric fruits, standing out States: Bahia, Minas Gerais, Rio Grande do Sul, Rio de Janeiro, São Paulo and Sergipe. With plantings above 1.100.000 hectares, the citrus have great commercial importance in Brazil, being São Paulo the largest producing with approximately 70% of the national production. The originating from economical return this product is of great importance for the growth of the agricultural section of the country. Starting from the crop of 1979-1981, the Brazilian citricultura is occupying the first place inside in production of South America. For such, the study was lead in the experimental field of the College of Engenharia Agrícola (FEAGRI) of the State University of Campinas, located in the city of Campinas, enters the geographic coordinates of 22o53¿20¿ of south Latitude and the meridian 47o04¿40¿ of Longitude west of Greenwich. The experiment was led in five plants of citrus of same variety (Citrus sinensis L. Osbeck), accomplishing perspiration measures, conductance stomatal, radiation fotossinteticamente activates, total incident radiation and deficit of pressure of saturated vapor. In the considered period it was observed that the largest average daily rate of the perspiration 4.63 mmol.m-2.s-1 in PL4T2 and the smallest was of 1.53 mmol.m-2.s-1 in PL1EVP1. The best model was that that considered that answer of the perspiration to foliate to be directly associated to the deficit of pressure of saturated vapor / Doutorado / Agua e Solo / Doutor em Engenharia Agrícola

Phenomenological models are presented which predicts transpiration rates (E) of individual leaves of Scaevola plumieri, an indigenous dune pioneer, and Ammophila arenaria, an exotic grass species introduced to stabilise mobile sand. In both cases E is predictably related to atmospheric vapour pressure deficit (VPD). VPD is calculated from measurements of ambient temperature and humidity, hence, where these two environmental variables are known, E can be calculated. Possible physiological reasons for the relationships of E to VPD in both species are discussed. Scaling from measurements of E at the leaf level to the canopy level is achieved by summing the leaf area of the canopy in question. E is predicted for the entire canopy leaf area by extrapolation to this larger leaf area. Predicted transpiration rates of individual shoot within the canopy were tested gravimetrically and shown to be accurate in the case of S. plumieri, but less so in the case of A. arenaria. Using this model, the amount of water used by a known area of sand dune is shown to be less than the rainfall input in the case of S. plumieri in wet and dry years. The water use of A. arenaria exceeds rainfall in the low-rainfall year of 1995, while in 1998 rainfall input is slightly higher than water extraction by the plants. Using a geographic information system (GIS), regional maps (surfaces) of transpiration were calculated from surfaces of mean monthly temperature and mean monthly relative humidity. Monthly surfaces of transpiration were subtracted from the monthly median rainfall to produce a surface of mean monthly water deficit. Areas of water surpluses along the coast correspond with the recorded distribution of both species in the seasons that the plants are most actively growing and reproducing. This suggests that unfavourable water availability during these two species growth periods limit their distributions along the coast. In addition to unfavourable water deficits, additional climatic variables that may be important in limiting the distribution of these two species were investigated using a discriminant function analysis.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The constant deficiency of available water resources and the society pressure for the rational water use in agriculture requires the correct management of the irrigation water. When a plant does not receive the right amount of water, it will affect the crop yield. The deficit irrigation contributes to increase the crops yield, comparing to a systems without irrigation, and as a results the agriculture will be more efficient preserving the quality ant quantity of natural recourses increasing or maintaining the crop yield. Therefore, this study is justified insofar as it seeks to identify deficit irrigation management in order to maintain satisfactory productivity, coupled with the water savings. The objective of this research was to evaluate water relations and gas exchange through the assessment of physiological variables that indicate changes in the crop yield of dry bean submitted to deficit irrigation. The experiment was conducted at the Federal University of Santa Maria, Brazil, under a mobile greenhouse, which was only closed during the occurrence of rain. In this way, there was no rainfall interference affecting the treatments. A completely randomized design was used with three replications. Treatments were consisted of four irrigation managements: 100% of accumulated crop evapotranspiration (ETc ac), and deficit irrigation of 75%, 50% and 25% of the ETc ac. Dry beans was sowed under a no tillage system, and the irrigation need was determined based on crop evapotranspiration values estimated using Penman-Monteith method, and the crop coefficients (Kc) used were those proposed by Allen et al. (1998). Irrigations were applied when the ETc accumulated a height of 20mm. The following leaf water relations and leaf gas exchanges were evaluated: Transpiration rate, stomatal conductance, leaf steam pressure deficit, internal concentration of CO2 and photosynthesis rate, using a LI-6400 (LI-Cor). With the values of photosynthesis rate, transpiration rate, stomatal conductance and internal concentration of CO2 were determined: transpiration instantaneous efficiency (photosynthesis rate/transpiration rate); intrinsic water use efficiency and (photosynthesis rate/stomatal conductance); carboxylation efficiency of the plants (photosynthesis rate/internal concentration of CO2). The following morphological features were evaluated: leaf area index, plant height and yield compounds (one hundred mass grains, number of seed plant and crop yield). The water use efficiency (EUA) was found by the ratio between grain yield (kg ha-1) and total depth applied (mm). During crop development accumulated ETc was 379,7 mm and was applied 122, 206, 290 and 376 mm of irrigation depth for the deficit irrigation treatment of 25%, 50%, 75% and 100% of ETc ac, respectively. The application of deficit irrigation reduced the leaf area index, plant height and reduction of 47,41% in the number of seed plant, 39,26%, in one hundred mass grain and 53,41% in crop yield. The reduction in water depth from 100% to 25% of ETc ac reduced by up to 91% of the stomatal concuctance and these plants had lower photosynthesis rate. The plants that received irrigation depth of 100% of ETc ac had higher E and carboxylation efficiency. The intrinsic water use efficiency and water use efficiency increased with the use of deficit irrigation. / A escassez dos recursos hídricos e a pressão social para o uso racional da água na agricultura exigem o correto uso e manejo da água de irrigação, pois, a falta ou excesso, comprometem o rendimento das culturas. O uso da irrigação, deficitária ou estratégica, contribui para o aumento da produtividade de grãos, comparando-se às condições de sequeiro, e resulta em uma agricultura economicamente mais eficiente, preservando os recursos naturais em termos de quantidade e qualidade, mantendo níveis satisfatórios de produtividade. Portanto, o presente estudo se justifica na medida em que se busca identificar manejos de irrigação deficitária que permitam manter níveis satisfatórios de produtividade, aliados à economia de água e energia. Assim, o objetivo desse trabalho foi avaliar as relações hídricas e as trocas gasosas, através da avaliação de variáveis fisiológicas que indiquem alterações na produção das plantas de feijão, quando submetidas a diferentes manejos de irrigação deficitária. O experimento foi desenvolvido na Universidade Federal de Santa Maria, Brasil, no interior de uma cobertura móvel, estruturada sobre trilhos metálicos, com movimentação mecânica, a qual somente foi fechada durante a ocorrência de chuvas. Dessa forma, não houve a interferência das chuvas durante a execução dos tratamentos. O delineamento experimental utilizado foi o inteiramente casualizado, com três repetições. Os tratamentos foram constituídos de quatro manejos de irrigação: reposição de 100% da evapotranspiração da cultura acumulada (ETc ac), e irrigação deficitária de 75%, 50% e 25% da ETc ac. A cultura do feijão foi semeada sob sistema de plantio direto e a necessidade de irrigação foi determinada com base na evapotranspiração da cultura, estimada pelo método de Penman-Monteith e os coeficientes de cultura (Kc) foram os propostos por Allen et al. (1998). Irrigações eram realizadas quando a ETc acumulava um valor de 20 mm. As seguintes relações hídricas e trocas gasosas das plantas foram avaliadas: taxa de transpiração, condutância estomática, déficit de pressão de vapor na folha, concentração interna de CO2 e taxa fotossintética, com o aparelho LI-6400 da Licor. Com os valores de taxa fotossintética, taxa de transpiração, condutância estomática e concentração interna de CO2 foram determinados: a eficiência instantânea da transpiração (razão taxa fotossintética/taxa de transpiração); a eficiência intrínseca do uso da água (razão taxa fotossintética/condutância estomática) e; a eficiência de carboxilação das plantas (razão taxa fotossintética/concentração interna de CO2). Foram avaliadas as seguintes características morfológicas nas plantas: índice de área foliar e altura de plantas e, os componentes de rendimento: massa de cem grãos, número de vagens por planta e rendimento de grãos. A eficiência de uso da água foi encontrada por meio da razão entre a produtividade de grãos (kg ha-1) e total de lâmina aplicada (mm). Durante o ciclo de desenvolvimento da cultura do feijão a ETc ac foi de 379,7 mm e foram aplicados 122, 206, 290 e 376 mm de lâmina de irrigação para os tratamentos de 25, 50, 75 e 100% da ETc ac, respectivamente. A aplicação da irrigação deficitária reduziu o índice de área foliar, a altura das plantas e causou reduções de até 47,41% no número de vagens por planta, 39,26%, na massa de cem grãos de feijão e 53,41% no rendimento de grãos. A redução da lâmina de irrigação de 100% para 25% da ETc ac reduziu em até 87% a condutância estomática das plantas e estas apresentaram menor taxa fotossintética. As plantas que receberam lâmina de irrigação de 100% da ETc ac apresentaram maiores taxa de transpiração e eficiência de carboxilação. A eficiência instrínseca do uso da água e a eficiência do uso da água aumentaram com a utilização da irrigação deficitária.

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Die Bewirtschaftung der knappen Ressource sauberen Wassers setzt das Verständnis der Wasserhaushaltsprozesse voraus. Mit prozessorientierten Modellen können Wasserbilanzen für unterschiedliche Standortbedingungen berechnet werden, wenn die Modelle zuvor an repräsentativen Zeitreihen, die die notwendige hydrologische Information enthalten, kalibriert und die Sensitivität ihrer Parameter analysiert wurden. Am Groß-Lysimeter St. Arnold (Westfalen) werden seit 1966 neben den meteorologischen Parametern tägliche Sickerwasserraten gemessen, die für diesen Zweck hervorragend geeignet sind, weil sie integrale Bilanzgrößen über die je 400m² x 3,50m großen Podsol-Bodenkörper und ihre Vegetationsbestände darstellen. Auf den drei Lysimetern wachsen Gras, ein Eichen-/Buchen- bzw. ein Kiefernbestand. Für die Untersuchung der Wasserhaushaltsprozesse wird das "LYsimeter outFlow and Evapotranspiration model" LYFE, entwickelt. Es verknüpft die Richards-Gleichung mit einem Interzeptionsmodell, das den Niederschlag in Infiltration, Blatt- und Streuinterzeption aufspaltet. Die Evapotranspirations-(ET)-Raten werden alternativ mit der Penman- oder Monteith-Formel berechnet. Die Simulationen vollziehen die gemessenen täglichen Sickerwasserraten aller drei Lysimeter unter den Klimavariabilitäten des gesamten Zeitraums von 34 Jahren nach. Am Graslysimeter ist die Sensitivität der ET-Parameter gering, so dass unterschiedlich aufwendige Methoden zur Bestimmung der bodenhydraulischen Parameter verglichen und bewertet werden können: die statischen Stechzylindermessungen der Retention und gesättigten Leitfähigkeit, der Verdunstungsversuch und verschiedene Pedotransferfunktionen (PTF). Die Simulation mit den Parametern des Verdunstungsversuchs ergeben eine gute Übereinstimmung mit den gemessenen Sickerwasserraten, während die Parameter der statischen Messungen durch die inverse Modellierung mindestens eines Parameters angepasst werden müssen. Von den PTF erzielen die kontinuierliche und die Klassen-PTF von Wösten die besten Übereinstimmungen. Der Wasserhaushalt der Baumbestände wird von der Interzeption dominiert. Dies gilt insbesondere für die Interzeptionsverdunstung im Winterhalbjahr, die die Unterschiedlichkeit der Wasserbilanz von Laub- und Nadelbaumbeständen verursacht. Wenn die ET mit der Penman-Formel berechnet wird, können die Raten der Evaporation und Transpiration nur schlecht abgeschätzt werden und steigen nicht mit dem Wachstum der Bestände. Durch die Verwendung der Monteith-Formel werden diese Probleme behoben. Darüberhinaus zeigt das Modell die unterschiedliche Wirkung von Transpiration und Interzeptionsverdunstung auf den Jahresgang der Sickerwasserraten und ermöglicht so die Angabe ihrer jeweiligen Beiträge zum Wasserhaushalt. Mit dem kalibrierten Modell können die lysimetrischen Messungen auf andere Standorte übertragen werden, um die langfristige Wasserbilanz zeitlich hochaufgelöst zu bestimmen. Daher kann LYFE im Rahmen regionaler Wasserhaushaltsuntersuchungen eingesetzt werden.

High latitude and altitude ecosystems are currently undergoing rapid and unprecedented warming in response to anthropogenically induced climate change. Subarctic, alpine regions are particularly vulnerable to increases in air temperature and changing precipitation regimes, which have caused cascading hydrological and ecological impacts. In addition to changing flow regimes, thawing permafrost, and declining glaciers, widespread changes in vegetation composition, density and distribution have been observed across northern regions. Specifically, treeline is advancing with increasing latitude and altitude and shrubs are increasing in height, extent, and density. Despite widespread documentation of this northern greening, few field-based studies have evaluated the hydrological implications of these changes. Quantification of total evapotranspiration (ET) across a range of vegetation gradients is essential for predicting water yield, yet challenging in cold alpine catchments due to heterogeneous land cover. Direct field-level measurements of transpiration (T) and evaporative partitioning across subarctic, alpine ecosystems and species are rare, yet essential to assess sensitivities and hydrological response to changing climate drivers. This thesis presents six years of surface energy balance components and ET dynamics and two years of sap flux measurements and critical zone stable water isotope sampling at three sites along an elevational gradient in a subarctic, alpine catchment near Whitehorse, Yukon Territory, Canada. These sites span a gradient of thermal and vegetation regimes, providing a space-for-time comparison for future ecosystem shifts: 1) a low-elevation boreal white spruce forest (~12-20 m), 2) a mid-elevation subalpine taiga comprised of tall, dense willow (Salix) and birch (Betula) shrubs (~1-3 m) and 3) a high-elevation subalpine taiga with short, sparse shrub cover (< 0.75 m) and moss, lichen, and bare rock. We utilize both mass flux measurements and stable water isotopes to evaluate the timing, magnitude, sensitivities, and sources of plant water uptake across these vegetation covers. Total ET decreased and interannual variability increased with elevation, with mean May to September ET totals of 349 (±3) mm at the forest, 249 (±10) mm at the tall, dense shrub site, and 240 (± 26) mm at the short, sparse shrub site. The shrub sites exhibited similar ET losses over 6 years despite differences in shrub height and abundance, although daily rates were higher at the tall shrub site in the peak growing season. From May to September, ET:R ratios were the highest and most variable at the forest (2.19 ± 0.37) and similar at the tall, dense shrub (1.22 ± 0.09) and short, sparse shrub (1.14 ± 0.05) sites. In the mid-growing season, mean T rates were greater at the dense shrub site (2.0 ± 0.75 mm d-1) than the forest (1.47 ± 0.52 mm d-1). During this time, T:ET was lower at the forest (0.48) than at the tall, dense shrub site (0.80). During the growing season between the two years, 2020 was considerably wetter and cooler than 2019. At the tall shrub site, during the mid-growing season (July 1-Aug 15), T dropped considerably in 2020 (-26%), as T was suppressed during the short, wet growing season. In contrast, T at the forest was only moderately suppressed (-3%) between years in this same period. Evapotranspiration was more strongly controlled by air temperature during the early and late season at the forest, while ET at the shrub site was more sensitive to warmer temperatures in the mid-growing season. At the shrub sites, ET was energy limited with no observed soil moisture limitation on T. While 2H and 18O of volume weighted precipitation became more depleted with elevation, the opposite was true in xylem water, where 2H and 18O became more enriched with elevation. Plant water uptake was more reflective of snow water at the forest site than both shrub sites, particularly early in the year and during dry periods. Near-surface bulk soil water had more negative lc-excess at the forest throughout the season and with depth, highlighting increased contributions from soil evaporation. This study combined direct measurements of sap flux, ET, and critical zone isotopes to provide new details on multi-year plant-soil-water dynamics, critical zone water cycling, and species-specific plant water uptake patterns in seasonally frozen soils, which have not previously been reported in cold regions. Our results suggest that advances in treeline will increase overall ET and lower interannual variability; however, the large growing season water deficit and stable water isotope signature at the forest indicates strong reliance on soil moisture from late fall and snowmelt recharge and the potential for plant water stress. Differences between the shrub species were apparent in the sap flux and stable isotope measurements, highlighting the need to further evaluate species specific responses and feedbacks when predicting hydrological fluxes across subarctic ecosystems. Overall, our results suggest that predicted changes in vegetation type and structure in northern regions will have a considerable impact on water partitioning and will vary in a complex way in response to changing precipitation timing, phase and magnitude. / Thesis / Candidate in Philosophy

Master of Science / Department of Agronomy / Robert M. Aiken / Mary B. Kirkham / Transpiration efficiency (TE) is defined as total biomass produced per unit of water transpired. Improvement of TE means maximizing crop production per unit of water used. The objectives of the study were to examine, at the leaf level and the whole plant level, the variation in TE for sorghum [Sorghum bicolor (L.) Moench] accessions, previously screened for TE and to test physiological mechanisms that may account for differences in TE. Three field studies and two mini-lysimeter studies (one done in pots under greenhouse conditions and one done in pots in the field) were conducted with eight accessions. Instantaneous measurements of assimilation (A), stomatal conductance (gs), and transpiration by gas exchange provided measures of the transpiration efficiency at the leaf level. Growth observations and soil water balance in field plots quantified components of whole-plant TE. Growth and development measurements showed significant difference, explaining the existence of photoperiod sensitivity among the sorghum genotypes. Assimilation (A), stomatal conductance (gs), and maximum quantum efficiency of photosystem II (Fv/Fm) were consistently greater for accession PI533946 (from India) and greater for accession PI295121 (from Australia) in both field and the field-pot studies (p<0.05). Internal carbon dioxide (Ci), an indicator of intrinsic transpiration efficiency, differed among lines under field conditions (p<0.05). Leaf relative water content (RWC), measured in the greenhouse, and did not differ among the eight accessions. No consistent differences in biomass and water use were detected among lines under field conditions. In conclusion, developing reliable selection indices for TE will require a greater understanding of whole-plant physiological processes to utilize the differences in TE observed at the leaf level.