P-aminobenzoic acid (PABA) is a polymorphic compound with two known polymorphs - alpha with a needle morphology and β with a rhombic morphology. It is an enantiotropic compound with a transition temperature at 13.8oC, where alpha is more thermodynamically stable above transition temperature and β is more thermodynamically stable below. At the beginning of this project, crash-cooling crystallisation experiments were conducted to determine the effect of solvent, temperature and supersaturation on the nucleating polymorphs of PABA. Three solvents were tested (water, ethanol and isopropyl alcohol) over a range of supersaturations and temperatures. The results suggested that polymorph appearance of PABA was heavily influenced by kinetics, as opposed to thermodynamics of the system, disagreeing with Ostwald's rule of stages. The project then focussed on the ability of tailor-made additives to select the crystallising polymorph of PABA from supersaturated solutions of PABA in isopropyl alcohol. Crash-cooling crystallisation experiments were performed using two additives: 4-amino-3-nitrobenzoic acid, and 4-amino-3-methoxybenzoic acid. Results showed that alpha PABA crystallised below a critical concentration of either additive, and above that critical concentration, β PABA would crystallise. To determine whether the additives were affecting the nucleation and/or growth kinetics of alpha PABA and β PABA, a series of nucleation and growth experiments were conducted using a Crystal16 multiple stirred reactor and a crystal growth cell respectively. The results showed that both additives greatly reduced the attachment frequency of growth units to alpha PABA nuclei, and inhibited the growth rate of alpha PABA seed crystals. Nucleation data could not be obtained for β PABA, but in terms of crystal growth, both additives did not affect growth rate of β PABA to a noticeable degree. Gravimetric and HPLC experiments were also employed to measure the solubility effects of both additives on PABA in isopropyl alcohol. Results showed that both additives did not appear to affect PABA's solubility to a noticeable degree.

The purpose of this study is to describe and analyze how companies offering tailor made products on the internet, can work with their marketing and their customer relationships. The study will answer the purpose from a service management perspective and there will also be presented theories from an Electronic Commerce perspective. The starting point for this study was the qualitative method. The essay builds its empirical material from interviews with knowledgeable persons in the subject and the owner of the company whose products are tailor made and sold on the internet. It was conducted seven interviews, four of which were face-to-face, two conducted by mail and finally there was made a telephone interview. Conclusions have been drawn based on the empirical material and theory. The study has been able to identify three concepts that are central to this type of product: Site-building, customer involvement and promotion & relationship management.

<p>The purpose of this study is to describe and analyze how companies offering tailor made products on the internet, can work with their marketing and their customer relationships. The study will answer the purpose from a service management perspective and there will also be presented theories from an Electronic Commerce perspective.</p><p>The starting point for this study was the qualitative method. The essay builds its empirical material from interviews with knowledgeable persons in the subject and the owner of the company whose products are tailor made and sold on the internet. It was conducted seven interviews, four of which were face-to-face, two conducted by mail and finally there was made a telephone interview.</p><p>Conclusions have been drawn based on the empirical material and theory. The study has been able to identify three concepts that are central to this type of product: Site-building, customer involvement and promotion & relationship management.</p>

The effect of impurities on crystal morphology is a challenging problem, since even at low concentrations they can have drastic effects on the final habit. Industrially this causes problems with downstream processes such as filtration, processability and even storage. Conversely, structurally related additive molecules may be introduced to a system in order to mimic the effect of an impurity resulting in a beneficial effect on problematic crystal morphologies. The work presented here considers the design and use of tailor made additives on a nonhydrogen bonded crystal, benzophenone. This compound is typical of many agrochemical materials in that the major intermolecular interactions are of the nondirectional van der Waals type. Using crystal packing analysis a selection of additives has been chosen with the intent of specifically hindering certain directions of crystal growth. From an initial group of nine molecules two additives, 4ABP and 4MBP were found tobe particularly effective, both strongly hindering growth. Measured kinetic data suggests that these additives bind to steps in the growth spirals, drastically slowing growth of specific crystal faces altering the crystal morphology to a needle shape. Through nucleation experiments and product analysis the additives were shown to effect only crystal growth becoming incorporated into the crystal structure. Computational modelling of the binding of additives to the crystal surfaces of benzophenone has been used in an attempt to rationalise the experimental effects. In many cases calculated binding energies were in agreement with experimental observation. However, modified attachment energies did not match well with experimental observations.

本論文研究的動機，乃因於中國大陸自1978年改革開放後，經濟呈現高度成長，目前更是全球最大新興市場和製造工廠，工程塑膠材料的需求及市場亦隨著3C電子塑膠零件和汽車塑膠零件在中國大量製造而蓬勃發展。但是，中國地區還是有許多法律環境不健全且市場機制不成熟; 近來更因為勞工意識高漲及當地的政策改革，台商在大陸的生存壓力愈來愈大。本研究欲藉由個案公司一路從經銷、研發及經營兩岸與工廠業務的過程，藉此探討個案公司在經營兩岸業務時，是如何審視自身的能力與條件，以及外在環境的發展機會與威脅，依此根據制定一套可行的成長策略，以獲得一個良好的績效產出，期待協助個案公司整體營運績效的提升。除此，本研究亦期望經此個案研究，可提供學術探討與企業實務相關的參考，有助了解工程塑膠科技材料產業成長策略之實況、困難與相關因應之道。 本研究為促使文獻探討的內容，更具週延性; 所以，首先從工程塑膠材料產業發展面開始了解，繼而從擬定策略所需的策略觀點進行探討，最後再從企業成長策略的擬定作探討。 本研究為探索性的個案與文獻之研究，所採取的研究架構，以企業願景與定位及大陸的競爭方式為自變數，而以企業成長策略為應變數。而研究方法，主要採行文獻探討、個案研究與資料分析。 對於個案公司的分析，首先從個案公司的沿革與產品範圍進行說明，其次對個案公司營運與型態進行探討，再者對個案公司所處之競爭與地位進行分析，最後對個案公司如何因應未來進行分解與剖析。 所以，本研究提出對於個案公司未來成長策略擬定的建議，主要從成長方向與策略規劃兩方面提出： 1.成長方向的建議: 質感的創造、標準的符合、環保的落實、異業的 結盟。 2.策略規劃的建議：成本領導策略(水平整合，海外拓展，紅海)差異化策 略:(量身訂做，研發集中，藍海) 對於未來從事相關議題的研究，本研究建議可從研究資料的增加、研究方法的改進與研究方向的拓展，以促使本領域的研究更深入與完整。

Heutzutage werden bereits klein- bis mittelformatige Fassadenplatten aus Textilbeton mit Flächen bis zu 6 m² im Bauwesen verwendet. Dabei werden die ca. 25 mm dicken Elemente mit netzartigen technischen Textilien aus AR-Glas bewehrt. In einem kürzlich abgeschlossenen Forschungsprojekt wurde eine großformatige Fassadenplatte aus Textilbeton mit einer Elementfläche von 12,2 m² entwickelt, die eine Plattendicke von nur 30 mm aufweist. Die Platte wurde mit zwei rückseitigen monolithisch verbundenen Betonstegen ausgesteift. Durch die Beschichtung der Textilien mit Epoxidharz konnten formstabile und formbare Bewehrungsstrukturen entwickelt werden, die Textilspannungen von bis zu 1400 N/mm² aufweisen. In einem Pilotprojekt wurden die Ergebnisse des Forschungsprojektes angewendet und ihre Praxistauglichkeit nachgewiesen. Der Artikel beschreibt das architektonische Konzept, gibt Hinweise zur konstruktiven Durchbildung und beschreibt Herstellverfahren der textilen Bewehrung und Fassadenplatte.

Les zéolithes représentent une importante classe de catalyseurs hétérogènes largement utilisées dans l’industrie. En effet, ce sont des catalyseurs de choix pour de multiples réactions grâce à leurs propriétés uniques largement modulables, à savoir leur forte acidité de Brønsted, leur grande surface spécifique, leur stabilité hydrothermique et leur sélectivité de forme. Cependant, la taille de leur micropores engendre des difficultés en terme de diffusion, réduisant ainsi leur potentiel catalytique. Ainsi, la conception sur mesure, en fonction de l’application finale, semble être une méthode très intéressante pour le développement de nouveaux catalyseurs zéolithiques.Les travaux réalisés au cours de cette Thèse ont pour objectif la conception de zéolithes à trois échelles : (i) au niveau du site actif en jouant sur la force acide et sur leur accessibilité, (ii) au niveau macroscopique par la taille des cristaux et (iii) au niveau du réacteur en les déposant sur divers supports. Ces différentes zéolithes ont été évaluées dans deux réactions acides : la chloration d’aromatiques et la conversion du méthanol en oléfines légères (MTO).Des zéolithes hiérarchisées ont été synthétisées selon diverses méthodes pré- ou post-Synthétiques et testées dans la chloration du nitrobenzène et du chlorobenzène. De plus, des cristaux « géant » de ZSM-5, préparés par voie fluorure, ont été évalués dans le MTO.La corrélation directe entre l’activité catalytique et les propriétés intrinsèques des zéolithes a permis de souligner les propriétés indispensables à chaque réactions. / Zeolites are aluminosilicate catalysts of great importance for the chemical industries. Their unique properties, i.e., strong Brønsted acidity, high surface area, high hydrothermal stability, and shape selectivity, combined with an extensive tunability, render them the candidate of choice in various reactions. Nevertheless, often only a fraction of their potential is exploited, due to access and diffusion limitation to/in their micropores. In this field, the rational design appears as a valuable method to design new zeolite catalysts, according to their targeted application.The work described in this Thesis aims in the design of zeolites at three levels: (i) acid site by playing on the acid strength and on the accessibility, (ii) microscopic scale by adapting the crystal size and (iii) reactor level by coating zeolite crystals on different supports. These as-Prepared zeolites were evaluated in two acid-Catalyzed reactions: the aromatics chlorination and the conversion of methanol in light olefins (MTO).Hierarchical zeolites were synthesized according to several pre- or post-Synthetic modifications and evaluated in nitro- and chlorobenzene chlorinations. Besides, “giant” ZSM-5 zeolites, prepared according to fluoride-Mediated route, as well as zeolite coatings on β-SiC, were tested in the MTO reaction.Thanks to a direct correlation between catalytic activities and intrinsic properties, optimal catalyst properties were highlighted in both reactions.

Heutzutage werden bereits klein- bis mittelformatige Fassadenplatten aus Textilbeton mit Flächen bis zu 6 m² im Bauwesen verwendet. Dabei werden die ca. 25 mm dicken Elemente mit netzartigen technischen Textilien aus AR-Glas bewehrt. In einem kürzlich abgeschlossenen Forschungsprojekt wurde eine großformatige Fassadenplatte aus Textilbeton mit einer Elementfläche von 12,2 m² entwickelt, die eine Plattendicke von nur 30 mm aufweist. Die Platte wurde mit zwei rückseitigen monolithisch verbundenen Betonstegen ausgesteift. Durch die Beschichtung der Textilien mit Epoxidharz konnten formstabile und formbare Bewehrungsstrukturen entwickelt werden, die Textilspannungen von bis zu 1400 N/mm² aufweisen. In einem Pilotprojekt wurden die Ergebnisse des Forschungsprojektes angewendet und ihre Praxistauglichkeit nachgewiesen. Der Artikel beschreibt das architektonische Konzept, gibt Hinweise zur konstruktiven Durchbildung und beschreibt Herstellverfahren der textilen Bewehrung und Fassadenplatte.

The present work demonstrates how a peptide-based material can be obtained for the biosorptive recovery of metals from contaminated industrial wastewater. Starting with Phage surface display for the initial identification and optimization of gallium-binding peptides, all the following application-focussed experiments are based on chemically synthesized peptides. Two chromatography-based biopanning methods for the identification of gallium-binding peptides from a commercial phage display library were developed. Five gallium-binding peptide sequences were identified and evaluated to show good gallium-binding properties. Furthermore, the biosorption of free gallium and arsenic by gallium-binding bacteriophage clones was investigated. A large influence of the pH-value on the respective interactions was demonstrated. Mutagenesis experiments were also carried out for a bacteriophage clone expressed peptide, in which a cysteine pair systematically replaced amino acids. Biosorption experiments with the resulting seven different bacteriophage mutants suggested a relationship between the rigidity of the peptide structure and the gallium-binding properties. In isothermal titration experiments, the thermodynamics of the interaction between gallium and the peptides as chemically synthesized derivatives were characterized, independent of the bacteriophage. The peptides differed strongly in their interaction with gallium, and in some cases, the complex formation with gallium depended strongly on the surrounding buffer conditions. The peptide with the amino acid sequence NYLPHQSSSPSR has particularly promising gallium-binding properties. Computer modeling suggests the probable structure of the peptide in aqueous solution and postulates a possible binding site for gallium. The side-selective and covalent immobilization of the peptides on a polystyrene matrix led to the creation of a biocomposite for the biosorptive recovery of gallium. The sorption performance and desorbability of the peptide-based biosorption materials were determined in studies with model solutions and real waters from the semiconductor industry.  :EIDESSTATTLICHE VERSICHERUNG II SUMMARY 7 CHAPTER I. 8 Utility of biotechnological approaches in resource technology 9 Phage Surface Display for the recovery of inorganic binding peptides 14 Gallium – Example of a high-tech metal 22 Aims and context of the present work 22 CHAPTER II. 25 Author contributions 25 Abstract 25 Introduction 26 Materials and Methods 28 2.1 Phage Display Library system 28 2.2 Biopanning experiments 29 2.3 Single clone identification 31 2.4 Single clone binding studies 31 Results 32 3.1 Immobilization of gallium ions 32 3.2 Biopanning experiments 33 3.3 Single clone binding studies 38 Discussion 39 4.1 Gallium ions as biopanning target 39 4.2 Phage clone selection 40 Conclusion 44 Acknowledgements 45 CHAPTER III. 46 Author contributions 46 Abstract 47 Introduction 47 Materials and Methods 49 2.1 Handling of phage display library clones 49 2.2 Site-directed mutagenesis experiments 50 2.3 Biosorption experiments 51 Results and Discussion 52 3.1 Experimental context 52 3.2 Original phage clone characterization 53 3.3 Site-directed mutagenesis experiments 56 3.4 Mutant phage clone characterization 57 Conclusions 59 Acknowledgements 60 CHAPTER IV. 61 Author contributions 61 Textual and graphical abstract 62 Introduction 63 Methods 65 2.1 Peptides 65 2.2 Isothermal titration microcalorimetry (ITC) 65 2.3 Preparation of peptide conjugates 65 2.4 Biosorption studies 66 2.5 Model calculation of peptide C3.8 67 Results and Discussion 68 3.1 Interaction studies of free peptides in solution 68 3.2 Biosorption studies with peptide polystyrene conjugates 71 3.2.1 Covalent and site-selective immobilization of peptides 71 3.2.2 Interaction of peptide conjugates with gallium 72 3.2.3 Interaction of peptide conjugates with arsenic 73 3.2.4 Continuous experiments 73 3.3 Model calculation for peptide C3.15 75 Counclusion 76 Acknowledgment 77 CHAPTER V. 78 Obtained insights for the selection of metal-binding peptides in biopanning experiments 79 Conclusions for the development of peptide-based materials for the biosorptive recovery of metal ions from aqueous solutions 81 REFERENCES 85 APPENDIX 94 SUPPORTING INFORMATION FOR CHAPTER IV 94 LIST OF FIGURES 99 LIST OF TABLES 100 LIST OF ABBREVIATIONS 101 LIST OF CHEMICALS 104 ACKNOWLEDGEMENTS 106 CURRICULUM VITAE 109 LIST OF PUBLICATIONS 111

The constitution of the Republic of South Africa has mandated government to keep in touch with the citizens, by regularly updating them on the services available for the improvement of their lives, and further to constantly report back on progress made in implementing government policies. In recent years, the country has been experiencing sporadic service delivery protests especially at the local sphere of government (municipal level). The aim of the study was therefore to examine the success of the communication and information system of the South African government in disseminating information to citizens. The area of the study was the Province of Mpumalanga which is one (1) of the nine (9) provinces constituting the Republic of South Africa. For the study, quantitative research method in the form of a survey was adopted, the hypothesis tested and the findings generalised. In creating a sample frame for the study, both probability and non-probability sampling techniques were adopted. The type of data collection instrument adopted was a structured questionnaire. Single-stage cluster sampling was adopted for heads of government communication whereas judgemental or purposive sampling technique was adopted for citizens. Data was analysed utilising data tabulation, descriptives, and data disaggregation quantitative data analysis procedures. The key findings of the study, amongst others, are: (a) That the current communication and information system being utilised by the South African government has an impact (it has improved communication with citizens); (b) That the citizens prefer government to communicate with them in their own indigenous languages (communicating in all eleven (11) official languages); (c) That the citizens prefer face-to-face communication; (d) That citizens would like to receive government messages via social media (given its immediacy and interactivity); and (e) That the low status of the communication function at the local government level denies communicators access to information to be communicated. Amongst others, the study recommends that (1) communication should be recognised as a strategic management function in all spheres of government; (2) the use of radio as the primary channel of information should be strengthened; (3) face-to-face communication with citizens should be strengthened; (4) information dissemination should be done in all eleven (11) official languages, including in Braille and sign-language; (5) social media and SMS notification should be included in the government communication policy as one of its official information dissemination channels. / Communication Science / M.A. (Communication)