A quantized control means that the control force is restricted to takes only a finite number of prescribed levels. The well-known bang-bang control or relay control belongs to this category. This kind of control has the advantage of simple circuit realization using electronic switches or relays that feature low power consumption in their on-off operation. However, quantized control introduces noise and distortion, and even worse its high nonlinearity makes the stabilizing compensator design difficult. This thesis applies the concept of dynamic sliding mode to the synthesis of a multi-level quantized control, with the aim to stabilize the system, perform reference tracking and attenuate the switching noise. The applicability of the presented sliding-mode quantized control is demonstrated on a three-level buck converter. Compared with the conventional PWM (Pulse-Width Modulation) scheme, it eliminates the use of a complex three-level PWM generator and a current sensor. A 12V/8V three-level buck converter with sliding mode quantized control is designed and realized, which shows the output voltage with 0.4625% of average DC error, 2.8988% of the static output ripple and 2.3% of load regulation error in response to the load current steps from 0A/3A to 3A/0A, at a slew rate of 6.25A/£gsec.

There are two main human pathogens in the family of Chlamydiaceae. Different serovars of Chlamydia trachomatis cause sexually-transmitted disease and eye infections whereas C. pneumoniae (TWAR) is a common cause of community-acquired respiratory infection. Chlamydia species are obligate, intracellular bacteria sharing a unique developmental cycle that occurs within a protected vacuole termed an inclusion. These microorganisms can be distinguished by two different forms: the infectious, metabolically inert elementary body (EB) and the reproducing non-infectious form, termed the reticulate body (RB). The cycle is terminated when re-differentiation of RBs back to infectious EBs occurs. Chlamydia possesses a type III secretion system (T3SS) essential for delivery of effector proteins into the host for host-cell interactions. This virulence system has been systematically characterized in several mammalian pathogens. Due to lack of a tractable genetic system for Chlamydia species, we have employed chemical genetics as a strategy to investigate molecular aspects of the T3SS. We have identified that the T3S-inhibitors INP0010 and INP0400 block the developmental cycle and interfere with secretion of T3S effector proteins in C. pneumoniae and C. trachomatis, without any cytotoxic effect. We have further shown that INP0010 decreases initiation of transcription in C. pneumoniae during the early mid-developmental cycle as demonstrated by a novel calculation, useful for measurement of transcription initiation in any intracellular pathogen. The mechanism regulating the signal(s) for primary as well as terminal differentiation of RBs has not been defined in Chlamydia. We show using T3S-inhibitors that INP0010 targets the T3SS and thereby arrests RB proliferation as well as RB to EB re-differentiation of C. pneumoniae as where INP0400 targets the T3SS and provokes a bacterial dissociation from the inclusion membrane presumed to mimic the natural occurrence of terminal differentiation. The effect of INP0010 on iron-responsive genes indicates a role for T3S in iron acquisition. Accordingly, our results suggest the possibility that C. pneumoniae acquires iron via the intracellular trafficking pathway of endocytosed transferrin. Moreover, we have for the first time presented data showing generalized bone loss from C. pneumoniae infection in mice. The infection was associated with increased levels of the bone resorptive cytokines IL-6 and IL-1beta. In addition, an increased sub-population of T-cells expressed RANKL during infection. Additionally, C. pneumoniae established an infection in a human osteoblast cell line in vitro with a similar cytokine profile as seen in vivo, supporting a causal linkage. Collectively, these data may indicate a previously unknown pathological role of C. pneumoniae in generalized bone loss.

This paper presents methods for photo-realistic rendering using strongly spatially variant illumination captured from real scenes. The illumination is captured along arbitrary paths in space using a high dynamic range, HDR, video camera system with position tracking. Light samples are rearranged into 4-D incident light fields (ILF) suitable for direct use as illumination in renderings. Analysis of the captured data allows for estimation of the shape, position and spatial and angular properties of light sources in the scene. The estimated light sources can be extracted from the large 4D data set and handled separately to render scenes more efficiently and with higher quality. The ILF lighting can also be edited for detailed artistic control.

Current Source Inverter (CSI) topology is gaining acceptance as a competitive alternative for grid interface of renewable energy systems due to its unique and advantageous features. Merits of CSI over the more popular Voltage Source Inverter (VSI) topology have been elaborated on by a number of researchers. However, there is a dearth of quality work in modeling and control of CSI topology interfacing renewable energy resources to the grid. To enrich the study focussing on application of CSI for renewable energy interface, this thesis develops a multilevel structure based on CSI for three-phase grid-connected Photovoltaic (PV) application. In the first part of research, a single-stage CSI interfacing to PV array is developed. The CSI-based PV system is equipped with Maximum Power Point Tracker (MPPT), DC-link current controller, and AC-side current controller. To eliminate the nonlinearity introduced by the PV array, a feed-forward control is introduced in the DC-link current controller. The AC-side current controller is responsible for maintaining unity power factor at the Point of Common Coupling (PCC). To verify the performance of the developed CSI-based PV system, a number of simulation studies are carried out in PSCAD/EMTDC environment. To illustrate the performance of the CSI-based PV system during transients on the grid side, simulation studies are carried out for four kinds of faults. Results obtained from fault studies are highly in favor of CSI topology and provide illustrative evidence for short-circuit current protection capability of the CSI. On the other hand, the VSI-based PV system performs poorly when subjected to similar grid transients. To extend the research on CSI-based PV system further, a multilevel structure based on CSI is developed. The multilevel structure is a parallel combination of $n$ CSI units and capable of producing $2n+1$ levels of current at the terminal of the inverter. Each unit in the multilevel structure has its own MPPT, DC-link current controller. However, on the AC-side a combined current controller is proposed. The design results in a high power rating with reduced number of filters, sensors and controllers. The developed multilevel structure can operate with PV arrays exposed to equal and unequal insolation level. However, when the PV arrays are operating under unequal insolation level, low order harmonics are generated in the sinusoidal current that is injected into the grid. Elimination of these harmonics is performed by implementing a modified control strategy in stationary reference frame that corresponds to the harmonic component that needs to be minimized. The modified control strategy operates in coordination with the existing DC-side and AC-side current controllers, and MPPTs. Therefore, real-time suppression of current harmonics can be ensured. Performance of the multilevel structure is verified by different transient studies.

This study examines the lapping machining process used during the manufacturing of polycrystalline diamond compacts (PDCs). More specifically, it is aimed at improving the productivity of the process by developing a better understanding of the parameters that affect the material removal rate (MRR) and MRR uniformity of lapped PDC samples. Experiments that focused on several controllable lapping parameters were performed to determine to what extent they affected the process. It was determined that the MRR can be modeled with the Preston equation under certain ranges of pressure and speed. It was also found that using a hard and rigid sample holder produces higher MRRs than soft and flexible sample holders. The results have also shown that MRRs in excess of 300 micrometers per hour can be achieved while using 10 grams of diamond abrasive per PDC per hour of lapping. The productivity of the lapping process can also be improved by placing the maximum allowed PDC samples in a concentric circle on the edge of the sample holder. The MRR uniformity between samples lapped on the same sample holder was found to be dependent on the sample holder material. This thesis is composed of six chapters. The first chapter introduces the need for PDC's as extreme cutting tools, the manufacturing process of PDC's, and the lapping process. The second chapter discusses the motivation behind this research and the primary objectives that were established. The third chapter details the materials and the experimental procedure, and the fourth chapter presents the results. The fifth chapter discusses the results, and the sixth chapter presents conclusions and information on possible future work.

A numerical analysis is performed for the three-dimensional, turbulent flow of air in a thyristor valve hall located at the Dorsey Converter Station, owned and operated by Manitoba Hydro. The goal of this analysis was to determine the configurations that result in increased air-side cooling effectiveness in the valve hall. The governing equations are solved using the Computational Fluid Dynamics (CFD) code CFX-5. This computer code uses a finite volume method of solution and is based on a finite element approach for representing the geometry. The effects of inlet and outlet opening geometry, inlet air mass flow rate, and inlet air angle on the thermal performance for the air-side cooling of the thyristor valve hall geometry are examined. / May 2006

Solid oxide fuel cells with graded electrodes consisting of multiple composite layers yield generally lower polarization resistances than single layer composite electrodes. Optimization of the performance of solid oxide fuel cells with graded electrode composition and/or microstructure requires an evaluation of both the three phase boundary length per unit volume and the effective diffusion coefficient in order to provide insight into how these properties vary over the design space. A numerical methodology for studying the three phase boundary length and effective diffusivity in composite electrode layers with controlled properties is developed. A three dimensional solid model of a sintered composite electrode is generated for which the mean particle diameter, composition, and total porosity may be specified as independent variables. The total three phase boundary length for the modeled electrode is calculated and tomographic methods are used to estimate the fraction of this length over which the electrochemical reactions can theoretically occur. Furthermore, the open porosity of the modeled electrode is identified and the effective diffusion coefficient is extracted from the solution of the concentration of the diffusing species within the open porosity. Selected example electrode models are used to illustrate the application of the methods developed, and the resulting connected three phase boundary length and diffusion coefficients are compared. A significant result is the need for thickness-specific effective diffusivity to be determined, rather than the general volume averaged property, for electrodes with porosity between the upper and lower percolation thresholds. As the demand for current increases, more of the connected three phase boundaries become active, and therefore a greater fraction of the electrode layer is utilized for a given geometry, resulting in a higher apparent effective diffusivity compared to the same electrode geometry operating at a lower current. The methods developed in this work may be used within a macroscopic electrode performance model to investigate optimal designs for solid oxide fuel cell electrodes with stepwise graded composition and/or microstructure.

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No description available.

The complexity of translation is a classical dilemma in the evolution of biological systems. Efficient translation requires coordination of complex, highly evolved RNAs and proteins; however, complex, highly evolved RNAs and proteins could not evolve without efficient translation system. At the heart of this complexity is the ribosome, itself a remarkably complex molecular machine. Our work illustrates the ribosome as deconstructed units of modification. Here we have deconstructed a segment of the ribosome to interacting RNA-protein units. L23 interacts in vivo with both Domain III (DIII) and Domain IIIcore (DIIIcore) independently of the fully assembled ribosome. This suggests that DIIIcore represents the functional rRNA unit in DIII-L23 interaction. Furthermore, L23peptide sustains binding function in vitro with both DIII and DIIIcore independently of any stabilizing effects from the globular domain of L23. The ability of L23peptide to form a 1:1 complex with both DIII and DIIIcore suggests that L23peptide is the functional rProtein unit in DIII-L23 interaction. We believe that our results will stimulate interest and discussions in the significance of 3D architecture and units of evolution in the ribosome. The ubiquity of the ribosome in cellular life prognosticates that our results impact and appeal to biologists, chemists, bioinformaticists, as well as the general scientific community.