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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
11

Mind the gap! : geographic transferability of economic evaluation in health

Boehler, Christian Ernst Heinrich January 2013 (has links)
Background: Transferring cost-effectiveness information between geographic domains offers the potential for more efficient use of analytical resources. However, it is difficult for decision-makers to know when they can rely on costeffectiveness evidence produced for another context. Objectives: This thesis explores the transferability of economic evaluation results produced for one geographic area to another location of interest, and develops an approach to identify factors to predict when this is appropriate. Methods: Multilevel statistical models were developed for the integration of published international costeffectiveness data to assess the impact of contextual effects on country-level; whilst controlling for baseline characteristics within, and across, a set of economic evaluation studies. Explanatory variables were derived from a list of factors suggested in the literature as possible constraints on the transferability of costeffectiveness evidence. The approach was illustrated using published estimates of the cost-effectiveness of statins for the primary and secondary prevention of cardiovascular disease from 67 studies and related to 23 geographic domains, together with covariates on data, study and country-level. Results: The proportion of variation at the country-level observed depends on the appropriate multilevel model structure and never exceeds 15% for incremental effects and 21% for incremental cost. Key sources of variability are patient and disease characteristics, intervention cost and a number of methodological characteristics defined on the data-level. There were fewer significant covariates on the study and country-levels. Conclusions: Analysis suggests that variability in cost-effectiveness data is primarily due to differences between studies, not countries. Further, comparing different models suggests that data from multinational studies severely underestimates country-level variability. Additional research is needed to test the robustness of these conclusions on other sets of cost-effectiveness data, to further explore the appropriate set of covariates, and to foster the development of multilevel statistical modelling for economic evaluation data in health.
12

We Know Better and It's Time to Act Like It: Ending Written Feedback

Rees, Jacob S. 13 March 2013 (has links)
Researchers have tried to demonstrate the effectiveness of written teacher feedback over the course of the last sixty years, and the results are inconclusive. Many studies point to improvement on subsequent drafts as evidence of student improvement; however, this only indicates students' abilities to follow directions. It is not an indication of autonomous writing ability. This study demonstrates that with proper curriculum support high school students can develop intentional transferability (the autonomous, intentional transferring of writing skills to varied rhetorical situations) throughout the course of one academic year without receiving any teacher written feedback.
13

Use of Advanced Techniques to Estimate Zonal Level Safety Planning Models and Examine their Temporal Transferability

Hadayeghi, Alireza 24 September 2009 (has links)
Historically, the traditional planning process has not given much attention to the road safety evaluation of development plans. To make an informed, defensible, and proactive choice between alternative plans and their safety implications, it is necessary to have a procedure for estimating and evaluating safety performance. A procedure is required for examining the influence of the urban network development on road safety, and in particular, determining the effects of the many variables that affect safety in urban planning. Safety planning models can provide a decision-support tool that facilitates the assessment of the safety implications of alternative network plans. The first objective of this research study is to develop safety planning models that are consistent with the regional models commonly used for urban transportation planning. Geographically weighted Poisson regression (GWPR), full-Bayesian semiparametric additive (FBSA), and traditional generalized linear modelling (GLM) techniques are used to develop the models. The study evaluates how well each model is able to handle spatial variations in the relationship between collision explanatory variables and the number of collisions in a zone. The evaluation uses measures of goodness of fit (GOF) and finds that the GWPR and FBSA models perform much better than the conventional GLM approach. There is little difference between the GOF values for the FBSA and GWPR models. The second objective of this research study is to examine the temporal transferability of the safety planning models and alternative updating methods. The updating procedures examine the Bayesian approach and application of calibration factors. The results show that the models are not temporally transferable in a strict statistical sense. However, relative measures of transferability indicate that the transferred models yield useful information in the application context. The results also show that the updated safety planning models using the Bayesian approach predict the number of collisions better than the calibration factor procedure.
14

Use of Advanced Techniques to Estimate Zonal Level Safety Planning Models and Examine their Temporal Transferability

Hadayeghi, Alireza 24 September 2009 (has links)
Historically, the traditional planning process has not given much attention to the road safety evaluation of development plans. To make an informed, defensible, and proactive choice between alternative plans and their safety implications, it is necessary to have a procedure for estimating and evaluating safety performance. A procedure is required for examining the influence of the urban network development on road safety, and in particular, determining the effects of the many variables that affect safety in urban planning. Safety planning models can provide a decision-support tool that facilitates the assessment of the safety implications of alternative network plans. The first objective of this research study is to develop safety planning models that are consistent with the regional models commonly used for urban transportation planning. Geographically weighted Poisson regression (GWPR), full-Bayesian semiparametric additive (FBSA), and traditional generalized linear modelling (GLM) techniques are used to develop the models. The study evaluates how well each model is able to handle spatial variations in the relationship between collision explanatory variables and the number of collisions in a zone. The evaluation uses measures of goodness of fit (GOF) and finds that the GWPR and FBSA models perform much better than the conventional GLM approach. There is little difference between the GOF values for the FBSA and GWPR models. The second objective of this research study is to examine the temporal transferability of the safety planning models and alternative updating methods. The updating procedures examine the Bayesian approach and application of calibration factors. The results show that the models are not temporally transferable in a strict statistical sense. However, relative measures of transferability indicate that the transferred models yield useful information in the application context. The results also show that the updated safety planning models using the Bayesian approach predict the number of collisions better than the calibration factor procedure.
15

The Marginal Social Worker : Exploring how Namibian social work students perceive and implement knowledge from a study exchange in Sweden.

Aisindi, Jonna January 2013 (has links)
This study explores how social work approaches and methods developed and practiced in one context can be interpreted, transferred and implemented in another. Additionally it focuses on how cultures and societies are organised both in individualistic and collectivistic societies and its effects on different approaches and conditions for social work. The data was collected from Namibian social work students that have participated in the Linnaeus Palme exchange program, which is an ongoing cooperation between the Swedish University West and University of Namibia. The results show that what the Namibian students foremost learn during their exchange studies in Sweden has to do with client interaction, critical thinking and dimensions of increased self-awareness. The assimilation of knowledge and possibilities to transfer and implement what they have learned in Sweden in a Namibian social work context is understood with the help of the theoretical concepts of the marginal man and transferability of knowledge. Shaped by the Namibian hybrid society, where the respondents are part of an ongoing negotiation between different antipoles and mediation between various cultural contexts, I argue that they are marginal social workers. Through their marginal experience they are in possession of qualities that are crucial in their process of transferability of knowledge and further create conditions for implementing new knowledge in the Namibian work practice.
16

An examination of the theory and practice of project management knowledge and its transferability in Western Australia

O'Shea, Keith January 2008 (has links)
Acceptance and understanding of project management tends to be limited by project managers (PMs) who have a specific technical training and follow one or two major theories on project processes. Consequently, project managers may encounter difficulty in being ‘recognised’ by employers and moving from one professional discipline to another. The current research examines knowledge areas applied ‘in the field’ by project managers (PMs), and provides them with knowledge about the constancy of application of project theories; further, a new model of what constitutes PM and enables flexibility for PMs is suggested. As there was little extant literature about PMs being able to transfer from one professional discipline to another, or to what degree different knowledge areas were applied, it was determined that exploratory research was appropriate. The results strongly indicate that organisations that rely on programs and projects to support the delivery of their strategic objectives will benefit from implementation of the project management revised hypothetical model (Figure 13). The model incorporates the nine knowledge areas of The Project Management Institute (USA) and six knowledge areas extracted from the PM methodology PRINCE2. Further, the additional focus of People Specific Management (PSM) at Level 3 of the revised hypothetical model (Figure 13) is recommended. Qualifications for PMs do make a difference! In addition to demonstrating the importance of PM qualifications to augment PM’s successful transfer across disciplines, further research is suggested regarding the need for training to be ‘compulsory’ and follow the developmental levels described in the up-to-date model developed during the research (Figure 13).
17

Can Species Distribution Models Predict Colonizations and Extinctions?

Venne, Simon 23 November 2018 (has links)
Aim MaxEnt, a very popular species distribution modelling technique, has been used extensively to relate species’ geographic distributions to environmental variables and to predict changes in species’ distributions in response to environmental change. Here, we test its predictive ability through time (rather than through space, as is commonly done) by modeling colonizations and extinctions. Location Continental U.S. and southern Canada. Time period 1979-2009 Major taxa studied Twenty-one species of passerine birds. Methods We used MaxEnt to relate species’ geographic distributions to the variation in environmental conditions across North America. We then modelled site-specific colonizations and extinctions between 1979 and 2009 as functions of MaxEnt-estimated previous habitat suitability and inter- annual change in habitat suitability and neighborhood occupancy. We evaluated whether the effects were in the expected direction, we partitioned model’s explained deviance, and we compared colonization and extinction model’s accuracy to MaxEnt’s AUC. Results IV Colonization and extinction probabilities both varied as functions of previous habitat suitability, change in habitat suitability, and neighborhood occupancy, in the expected direction. Change in habitat suitability explained very little deviance compared to other predictors. Neighborhood occupancy accounted for more explained deviance in colonization models than in extinction models. MaxEnt AUC correlates with extinction models’ predictive ability, but not with that of colonization models. Main conclusions MaxEnt appears to sometime capture a real effect of the environment on species’ distributions since a statistical effect of habitat suitability is detected through both time and space. However, change in habitat suitability (which is much smaller through time than through space) is a poor predictor of change in occupancy. Over short time scales, proximity of sites occupied by conspecifics predicts changes in occupancy just as well as MaxEnt. The ability of MaxEnt models to predict spatial variation in occupancy (as measured by AUC) gives little indication of transferability through time. Thus, the predictive value of species distribution models may be overestimated when evaluated through space only. Future prediction of species’ responses to climate change should make a distinction between colonization and extinction, recognizing that the two processes are not equally well predicted by SDMs.
18

Identificação, perfil fenotípico e disseminação clonal de cepas de Acinetobacter spp. em hospitais do estado do Rio de Janeiro / Identification, phenotypic profile and clonal spread of strains of acinetobacter spp. hospitals in the state of Rio de Janeiro

Luciene Ribeiro da Costa Silva 10 August 2010 (has links)
Espécies do gênero Acinetobacter são patógenos oportunistas que têm sido associados a várias infecções relacionadas à assistência em saúde acometendo principalmente, pacientes hospitalizados em centros de tratamento intensivo. A. baumannii , Acinetobacter genoespécie 3 e Acinetobacter genoespécie 13TU constituem o complexo A. baumannii e são consideradas as espécies de maior importância clínica. O objetivo deste trabalho foi identificar em nível de espécie, avaliar o perfil de resistência e analisar a diversidade genética de 102 amostras de Acinetobacter spp. isoladas de hemoculturas de pacientes internados em quatro hospitais do estado do Rio de Janeiro. Após a utilização de duas técnicas moleculares, 87 (85,3%) amostras foram identificadas como A. baumannii, sete (6,9%) como A. genoespécie 3, duas (1,9%) A. genoespécie 13TU e seis (5,9%) não foram identificadas em nível de espécie. A maioria das amostras de A. baumannii apresentou caráter multirresistente mostrando percentuais de resistência acima de 70% para ceftazidima, cefotaxima e ciprofloxacina. A resistência aos carbapenêmicos variou de 59% a 91%. Foi encontrada uma grande variedade de antibiotipos entre as amostras de A. baumannii, sendo prevalente dois multirresistentes. Um deles, caracterizado pela sensibilidade apenas aos aminoglicosídeos, ocorreu em 20,7% das amostras e o outro observado em 14,9% das amostras , foi caracterizado pela resistência a todos os antimicrobianos testados. Através da PCR, foi observado que 77% das amostras de A. baumannii apresentaram produto de amplificação compatível com gene blaOXA-23-like e destas, 64 mostraram-se resistentes tanto a imipenem quanto a meropenem. Em contrapartida, todas as amostras de A. baumannii OXA-23 negativas mostraram-se sensíveis aos carbapenens. Em relação às amostras de A. genoespécie 3 e 13TU, foram observados baixos percentuais de resistência frente aos antimicrobianos testados e apenas uma amostra de Acinetobacter genoespécie 3 apresentou produto de amplificação compatível com gene blaOXA-23-like, sendo esta sensível aos carbapenens. Não foram detectados os genes blaOXA-40-like e blaOXA-58-like nas 102 amostras de Acinetobacter spp.. A análise do polimorfismo genético das amostras de A. baumannii por PFGE mostrou a presença de 35 clones distribuídos entre os hospitais. Um clone (designado A), presente em 32 amostras (36,9%), foi encontrado nos quatro hospitais, sendo prevalente em três. Em 93,8% das amostras do clone A foi detectado o gene blaOXA-23-like. A disseminação de um clone de A. baumannii multirresistente produtor de OXA-23 entre os hospitais estudados evidencia a importância de medidas de controle de infecções mais eficazes, visando minimizar a morbidade e a mortalidade causadas por este importante patógeno. Além disso, como outras espécies também podem estar associadas a infecções, destacamos a importância da identificação correta das amostras em nível de espécie, visando o conhecimento da patogenicidade, do perfil de resistência e dados epidemiológicos des outras espécies, principalmente as pertencentes ao complexo A. baumannii / Acinetobacter species are opportunistic pathogens that have been associated with wide variety infections related to health care affects mainly patients hospitalized in intensive care units. A. baumannii and its phenotypically related species (Acinetobacter genoespécie 3 and genoespécie 13TU), together forming the A.baumannii complex and are considered species of greatestclinical importance. The objective of this study was to identify at the species level, to know the resistance profile and analyze the genetic diversity of 102 samples of Acinetobacter spp. isolated from blood cultures of patients admitted to four hospitals in the state of Rio de Janeiro. After using two molecular techniques, 87 (85.3%) were identified as A. baumannii, seven (6.9%) as A. genoespécie 3, two (1.9%) A. genoespécie 13TU and six (5.9%) were not identified at the species level. The most specimens of A. baumannii presented multidrug resistance, showing resistance rates above 70% for ceftazidime, cefotaxime and ciprofloxacin. The carbapenem resistance ranged from 59% to 91%. There was a wide variety of antibiotype between samples of A. baumannii, with two prevalent multiresistant antibiotypes. One, characterized by sensitivity only to aminoglycosides occurred in 20.7% of the samples and the other (14.9% of the samples) characterized by resistance to all antimicrobials tested. By PCR, we observed that 77% of the samples of A. baumannii showed amplification product consistent with gene blaOXA-23-like and of these, 64 were resistant to both imipenem and meropenem. In contrast, all samples of A. baumannii OXA-23 negative were sensitive to carbapenems. In samples of A. genoespécie 3 and 13TU were observed low percentages of resistance against the tested antimicrobials and only a sample of Acinetobacter genoespécie 3 showed amplification product consistent with blaOXA-23-like, which was sensitive to carbapenens. The genes blaOXA-40-like and blaOXA-58-like were not detected in 102 samples of Acinetobacter spp.. Analysis of genetic polymorphism of the samples of A. baumannii by PFGE showed the presence of 35 clones distributed among the hospitals. A clone (designated A), present in 32 samples (36.9%) was found in four hospitals. In 93.8% of the samples inclued clone A were detected the gene blaOXA-23-like. The spread of a clone multidrug-resistant A. baumannii producing the OXA-23 enzyme in the four hospitals showed the importance of infections control measures more effective, in order to minimize morbidity and mortality caused by this important pathogen. Moreover, as other species may also can be associated with infections, we showed the importance of correct identification of the samples at the species level, for the knowledge of the pathogenicity.The resistance profile and epidemiological study of species of Acinetobacter other than A. baumannii, especially those belonging to the Complex A. baumannii
19

Identificação, perfil fenotípico e disseminação clonal de cepas de Acinetobacter spp. em hospitais do estado do Rio de Janeiro / Identification, phenotypic profile and clonal spread of strains of acinetobacter spp. hospitals in the state of Rio de Janeiro

Luciene Ribeiro da Costa Silva 10 August 2010 (has links)
Espécies do gênero Acinetobacter são patógenos oportunistas que têm sido associados a várias infecções relacionadas à assistência em saúde acometendo principalmente, pacientes hospitalizados em centros de tratamento intensivo. A. baumannii , Acinetobacter genoespécie 3 e Acinetobacter genoespécie 13TU constituem o complexo A. baumannii e são consideradas as espécies de maior importância clínica. O objetivo deste trabalho foi identificar em nível de espécie, avaliar o perfil de resistência e analisar a diversidade genética de 102 amostras de Acinetobacter spp. isoladas de hemoculturas de pacientes internados em quatro hospitais do estado do Rio de Janeiro. Após a utilização de duas técnicas moleculares, 87 (85,3%) amostras foram identificadas como A. baumannii, sete (6,9%) como A. genoespécie 3, duas (1,9%) A. genoespécie 13TU e seis (5,9%) não foram identificadas em nível de espécie. A maioria das amostras de A. baumannii apresentou caráter multirresistente mostrando percentuais de resistência acima de 70% para ceftazidima, cefotaxima e ciprofloxacina. A resistência aos carbapenêmicos variou de 59% a 91%. Foi encontrada uma grande variedade de antibiotipos entre as amostras de A. baumannii, sendo prevalente dois multirresistentes. Um deles, caracterizado pela sensibilidade apenas aos aminoglicosídeos, ocorreu em 20,7% das amostras e o outro observado em 14,9% das amostras , foi caracterizado pela resistência a todos os antimicrobianos testados. Através da PCR, foi observado que 77% das amostras de A. baumannii apresentaram produto de amplificação compatível com gene blaOXA-23-like e destas, 64 mostraram-se resistentes tanto a imipenem quanto a meropenem. Em contrapartida, todas as amostras de A. baumannii OXA-23 negativas mostraram-se sensíveis aos carbapenens. Em relação às amostras de A. genoespécie 3 e 13TU, foram observados baixos percentuais de resistência frente aos antimicrobianos testados e apenas uma amostra de Acinetobacter genoespécie 3 apresentou produto de amplificação compatível com gene blaOXA-23-like, sendo esta sensível aos carbapenens. Não foram detectados os genes blaOXA-40-like e blaOXA-58-like nas 102 amostras de Acinetobacter spp.. A análise do polimorfismo genético das amostras de A. baumannii por PFGE mostrou a presença de 35 clones distribuídos entre os hospitais. Um clone (designado A), presente em 32 amostras (36,9%), foi encontrado nos quatro hospitais, sendo prevalente em três. Em 93,8% das amostras do clone A foi detectado o gene blaOXA-23-like. A disseminação de um clone de A. baumannii multirresistente produtor de OXA-23 entre os hospitais estudados evidencia a importância de medidas de controle de infecções mais eficazes, visando minimizar a morbidade e a mortalidade causadas por este importante patógeno. Além disso, como outras espécies também podem estar associadas a infecções, destacamos a importância da identificação correta das amostras em nível de espécie, visando o conhecimento da patogenicidade, do perfil de resistência e dados epidemiológicos des outras espécies, principalmente as pertencentes ao complexo A. baumannii / Acinetobacter species are opportunistic pathogens that have been associated with wide variety infections related to health care affects mainly patients hospitalized in intensive care units. A. baumannii and its phenotypically related species (Acinetobacter genoespécie 3 and genoespécie 13TU), together forming the A.baumannii complex and are considered species of greatestclinical importance. The objective of this study was to identify at the species level, to know the resistance profile and analyze the genetic diversity of 102 samples of Acinetobacter spp. isolated from blood cultures of patients admitted to four hospitals in the state of Rio de Janeiro. After using two molecular techniques, 87 (85.3%) were identified as A. baumannii, seven (6.9%) as A. genoespécie 3, two (1.9%) A. genoespécie 13TU and six (5.9%) were not identified at the species level. The most specimens of A. baumannii presented multidrug resistance, showing resistance rates above 70% for ceftazidime, cefotaxime and ciprofloxacin. The carbapenem resistance ranged from 59% to 91%. There was a wide variety of antibiotype between samples of A. baumannii, with two prevalent multiresistant antibiotypes. One, characterized by sensitivity only to aminoglycosides occurred in 20.7% of the samples and the other (14.9% of the samples) characterized by resistance to all antimicrobials tested. By PCR, we observed that 77% of the samples of A. baumannii showed amplification product consistent with gene blaOXA-23-like and of these, 64 were resistant to both imipenem and meropenem. In contrast, all samples of A. baumannii OXA-23 negative were sensitive to carbapenems. In samples of A. genoespécie 3 and 13TU were observed low percentages of resistance against the tested antimicrobials and only a sample of Acinetobacter genoespécie 3 showed amplification product consistent with blaOXA-23-like, which was sensitive to carbapenens. The genes blaOXA-40-like and blaOXA-58-like were not detected in 102 samples of Acinetobacter spp.. Analysis of genetic polymorphism of the samples of A. baumannii by PFGE showed the presence of 35 clones distributed among the hospitals. A clone (designated A), present in 32 samples (36.9%) was found in four hospitals. In 93.8% of the samples inclued clone A were detected the gene blaOXA-23-like. The spread of a clone multidrug-resistant A. baumannii producing the OXA-23 enzyme in the four hospitals showed the importance of infections control measures more effective, in order to minimize morbidity and mortality caused by this important pathogen. Moreover, as other species may also can be associated with infections, we showed the importance of correct identification of the samples at the species level, for the knowledge of the pathogenicity.The resistance profile and epidemiological study of species of Acinetobacter other than A. baumannii, especially those belonging to the Complex A. baumannii
20

Transferibilidade e validação de marcadores microssatélites derivados de EST para duas espécies de Campomanesia (Myrtaceae) do Cerrado / Transferability and validation of EST - derived microsatellite markers for two species of Campomanesia (Myrtaceae) from the Savanna

Miranda, Elen Amoreli Gonçalves Cintra 02 June 2014 (has links)
Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2016-05-19T21:05:23Z No. of bitstreams: 2 Dissertação - Elen Amoreli Gonçalves Cintra Miranda - 2014.pdf: 2511663 bytes, checksum: 82dcd84be188d0a4232f300033b12581 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-05-20T14:05:11Z (GMT) No. of bitstreams: 2 Dissertação - Elen Amoreli Gonçalves Cintra Miranda - 2014.pdf: 2511663 bytes, checksum: 82dcd84be188d0a4232f300033b12581 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2016-05-20T14:05:11Z (GMT). No. of bitstreams: 2 Dissertação - Elen Amoreli Gonçalves Cintra Miranda - 2014.pdf: 2511663 bytes, checksum: 82dcd84be188d0a4232f300033b12581 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-06-02 / The Savanna is highly diverse and has the richest flora and endemic in the world, comprising about 12,000 species of native plants. The family Myrtaceae is among the 10 families representative of this biome, comprising 14 genera. Among these, gender Campomanesia has several fruit species, known popularly as gabiroba. The species Campomanesia adamantium and Campomanesia pubescens have widespread occurrence in the Savanna. Its fruits can be consumed " in nature ", in the form of candy, ice cream , soft drinks and as flavoring in alcoholic distillates, besides having antidiarrheal properties, its bark and leaves are used in the form of teas. His exploration is by extractivism and can also be grown in small family orchards, being a source of extra income for some families. Thus, the study of genetic diversity and genetic structure of populations’ allies with other areas of knowledge can provide important information for planning and execution of native species conservation programs information. Currently, molecular markers have wide application for studies on the genetics of populations, among the types of markers used; microsatellites have been widely used in recent years by high power to detect genetic variability. Population - genetic studies of the genus Campomanesia sp. are scarce and so not found microsatellite markers available in the literature for any species of the Savanna o. In this context, the aim of this study was to test the potential for transferability of microsatellite markers derived gene regions (EST) developed for Eucalyptus sp., Campomanesia in two species (C. adamantium and C. pubescens), providing a panel of polymorphic markers for the species. To do so, we sampled two populations of each species and DNA was extracted from leaf tissue. The potential for transferability of 120 primers pairs were evaluated. The PCR products derived from the microsatellite markers were assessed in automatic transferred DNA analyzer. The array of genotypes was used to estimate the parameters of genetic variability for the panel of marker. Of the 120 primers tested, 87 were discarded for not having amplification products, many nonspecific fragments amplified 22 and 12 were considered successfully transferred the two species. The 12 markers transferred showed good discrimination power of the individual , with high probability of paternity exclusion (0,99939 to C. adamantium and 0,99982 for C. pubescens) and low probability of identity (5,718 x 10-10 for C. adamantium and 1,182 x 10-11 to C. pubescens). The average number of alleles at polymorphic loci was equal to 6,8 to C. adamantium, ranging from 2 (EMBRA1335 and EMBRA 1811) 16 (EMBRA 1364) and C. pubescens was 7,8 ranging from 2 (EMBRA1811 and EMBRA 1076) to 16 (EMBRA 2011) alleles per locus. Mean genetic diversity of Nei (1973) in populations was equal to 0,517 (C. adamantium) and 0,579 (C. pubescens). Mean observed heterozygosity (Ho) for the populations was equal to 0,505 (C. adamantium) and 0,503 (C. pubescens). For some markers for deviations expected for the Hardy-Weinberg equilibrium was observed due proportion’s estimates of intrapopulation inbreeding (f) were not significant for any population. Inbreeding full level (F) was significantly different from zero, suggesting some genetic variability structure populations. The structure of genetic variability in component among populations (θp) was equal to 0,105 (C. adamantium) and 0,249 (C. pubescens). / O Cerrado apresenta uma grande diversidade e possui a mais rica e endêmica flora do mundo, abrigando cerca de 12.000 espécies de plantas nativas. A família Myrtaceae está entre as 10 famílias representativas deste bioma, composta por 14 gêneros. Dentre estes, o gênero Campomanesia apresenta várias espécies frutíferas, conhecidas popularmente como gabiroba. As espécies Campomanesia adamantium e Campomanesia pubescens apresentam ampla ocorrência no Bioma Cerrado. Os seus frutos podem ser consumidos “in natura”, na forma de doces, sorvetes, refrescos e como flavorizantes em destilados alcoólicos, além de possuírem propriedades antidiarréicas, suas cascas e suas folhas são usadas sob a forma de chás. Sua exploração ocorre por extrativismo e, também, pode ser cultivada em pequenos pomares familiares, sendo uma fonte de renda extra para algumas famílias. Desta forma o estudo da diversidade genética e estrutura genética de populações aliados com outras áreas do conhecimento, pode fornecer informações importantes para o planejamento e execução de programas de conservação de espécies nativas. Atualmente, os marcadores moleculares têm ampla aplicação para estudos referentes à genética de populações. Dentre os tipos de marcadores utilizados, os microssatélites têm sido amplamente utilizados nos últimos anos, pelo alto poder de detecção da variabilidade genética. Estudos genéticos-populacionais com espécies do gênero Campomanesia sp. são escassos e não foram encontrados marcadores microssatélites disponíveis na literatura para nenhuma espécie do Cerrado. Nesse contexto, a proposta deste trabalho foi testar o potencial de tranferibilidade de marcadores microssatélites provinientes de regiões gênicas (EST) desenvolvidos para Eucalyptus sp., em duas espécies de Campomanesia (C. adamantium e C. pubescens), disponibilizando um painel de marcadores polimórficos para as espécies. Para tanto, foram amostradas duas populações de cada espécie e o DNA foi extraído a partir de tecido foliar. Foi avaliado o potencial de transferibilidade de 120 pares primers. Os produtos de PCR derivados dos marcadores microssatélites transferidos foram avaliados em analisador automático de DNA. A matriz de genótipos foi utilizada para estimar os parâmetros de variabilidade genética para o painel de marcadores. Dos 120 primers testados, 87 foram descartados por não apresentarem produtos de amplificação, 22 amplificaram muitos fragmentos inespecíficos e 12 foram considerados transferidos com sucesso para as duas espécies. Os 12 marcadores transferidos apresentaram bom poder de discriminação individual, com alta probabilidade de exclusão de paternidade (0,99939 para C. adamantium e 0,99982 para C. pubescens) e baixa probabilidade de identidade (5,718 x 10-10 para C. adamantium e 1,182 x 10-11 para C. pubescens). O número médio de alelos nos locos polimórficos foi igual a 6,8 para C. adamantium, variando entre 2 (EMBRA 1335 e EMBRA 1811) a 16 (EMBRA 1364) e em C. pubescens foi de 7,8 variando entre 2 (EMBRA 1811 e EMBRA 1076) a 16 (EMBRA 2011) alelos por loco. A média da diversidade genética de Nei (1973) nas populações foi igual a 0,517 (C. adamantium) e 0,579 (C. pubescens). A heterozigosidade média observada (Ho) para as populações foi igual a 0,505 (C. adamantium) e 0,503 (C. pubescens). Para alguns marcadores foi observado desvios para as proporções esperados para o equilíbrio Hardy-Weinberg. As estimativas de endogamia intrapopulacional (f) não foram significativas para nenhuma das populações. A endogamia total (F) foi significativamente diferentes de zero, sugerindo certa estruturação da variabilidade genética das populações. A estruturação da variabilidade genética no componente entre populações (θp) foi igual a 0,105 (C. adamantium) e 0,249 (C. pubescens).

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