O núcleo hipotalâmico supraquiasmático (SCh), apresenta caracterização neuroquímica com duas subpopulações principais de células, a produtora de polipeptídeo intestinal vasoativo (VIP) e argenina vasopressina (AVP). As fibras IR AVP e VIP oriundas do SCh apresentam características morfológicas específicas que possibilitam o rastreamento a longas distâncias dentro do hipotálamo. No presente estudo buscamos mapear os terminais IR VIP e AVP nas áreas hipotalâmicas do primata Cebus apella utilizando a imuno-histoquímica, e fazer a identificação das áreas hipotalâmicas recipientes do SCh, utilizando o mapeamento da distribuição das fibras IR associado à análise morfológica destas duas substâncias neuroativas. As fibras IR VIP e AVP com características do SCh foram identificadas em porções anteriores como hipotálamo anterior, área pré-óptica, área hipotalâmica lateral, SPZV, até porções mais caudais, porção retroquiasmática, área tuberal. Os resultados indicam um padrão similar de distribuição de fibras IR VIP e AVP nas áreas do hipotálamo e também em áreas descritas como recipientes das projeções do SCh, quando comparado com outras espécies como roedores descritos na literatura. / The suprachiasmatic nucleus (SCN) of the hypothalamus, contains a variety of different neurons that tend to form two major subpopulations within the nucleus, the vasoactive intestinal peptide (VIP) and vasopressin (VP). The immunoreactive (IR) fibers derived from the VIP and VP IR cells of the SCN present morphological characteristics that allow their specific tracking in long distances within the hypothalamus. In the present investigation we aimed map VIP and VP IR terminals in hypothalamic areas of the primate Cebus apella using immunohistochemistry, and to do identification of hypothalamic recipient areas from SCN using the mapping distribution of fibers IR associated with morphological analysis of these two neuroactive substances. VIP and VP IR fibers with characteristics from SCN were identified in the rostral anterior hypothalamic area and medial preoptic area, laterally to the lateral hypothalamic area, and more caudally in SPZV and retrochiasmatic tuberal area. The results indicate that there is a similarity in the pattern of distribution of VIP and VP fibers in the hypothalamic areas and also in areas recipients from SCN projections when compared with nocturnal rodent species described in the literature.

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-02-01T07:36:16Z No. of bitstreams: 2 Tese - Mariana da Silva Godinho - 2014.pdf: 11659350 bytes, checksum: 36a0ea84b6d0c992d5629322e89390a3 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-02-01T07:42:02Z (GMT) No. of bitstreams: 2 Tese - Mariana da Silva Godinho - 2014.pdf: 11659350 bytes, checksum: 36a0ea84b6d0c992d5629322e89390a3 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2016-02-01T07:42:02Z (GMT). No. of bitstreams: 2 Tese - Mariana da Silva Godinho - 2014.pdf: 11659350 bytes, checksum: 36a0ea84b6d0c992d5629322e89390a3 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-06-27 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / The lifetime of a power transformer is determined by the state of degradation of its insulation system. This system consists of Kraft paper immersed in insulating oil (IMO). In order to prevent an abrupt crash transformer its oxidation state should be monitored. This monitoring is performed using physico-chemical and chromatographic assays. The physico-chemical parameter of greatest importance is the interfacial tension (IT), which is a measure for assessing indirectly the oxidation state of the Kraft paper. The objective of this thesis to propose analytical methodologies to evaluate the oxidation process of the insulation system, both in naturally and accelerated degraded oil samples, using Image Analysis and spectroscopic techniques combined with methods of Multivariate Calibration (Partial Least Squares - PLS), Resolution Multivariate Curve and Data Fusion. Spectra of Moleculular Fluorescence (MF), Nuclear Magnetic Resonance of 1H (1H NMR), Fourier Transform Near Infrared (FT-NIR) and RGB images were obtained for a set of 268 oil samples provided by CELG Distribuição S.A. (CELG D). Subsequently, spectra in the Ultraviolet-Visible (UV-Vis) region, FM and RGB images were obtained for a set of 24 oil samples subjected to an accelerated degradation. PLS models were obtained in order to predict the IT and color parameters for the set of samples provided by CELG D. For the set of degraded samples PLS models were obtained for prediction of degradation time. PARAFAC and MCR-ALS models were obtained to evaluate the oxidation process of the insulating system and too identify the pure spectra of possible compounds formed during this process. Furthermore, low level data fusion combined with variable selection using the Variables Importance in the Projection (VIPs) was used in this study to obtain PLS models. The best PLS model for the set of samples provided by CELG D was obtained for the fused spectroscopic data using variable selection by VIPS. This model provided a mean square error of prediction (RMSEP) of 2.9 mN/m for IT and 0.3 for the color parameter (dimensionless units). PARAFAC and MCR-ALS models allowed to assess the process of system degradation. The proposed methodologies are fast and non-destructive, showing great advantages over traditional methods, which take a long time, and generate a lot of waste. This work opens perspectives for an online monitoring to assess the degradation of the insulating system besides having permitted a more detailed study on the compounds formed during the degradation process. / A vida útil de um transformador de potência é determinada pelo estado de degradação de seu sistema isolante. Esse sistema é constituído pelo papel tipo Kraft imerso em óleo mineral isolante (OMI). Para evitar que um transformador chegue ao seu fim de vida de forma abrupta é necessário que seja realizado o monitoramento do seu estado de oxidação. Esse monitoramento é feito através de ensaios físico-químicos e cromatográficos. O parâmetro físico-químico de maior importância é a tensão interfacial (TI), pois é uma medida que avalia indiretamente o estado de oxidação do papel. O objetivo deste trabalho foi propor metodologias analíticas para avaliar o processo de degradação do sistema isolante tanto em amostras degradadas naturalmente quanto em amostras degradadas aceleradamente, usando Análise de Imagens e técnicas espectroscópicas aliadas a métodos de Calibração Multivariada (Mínimos Quadrados Parciais - PLS), Resolução de Curva Multivariada e Fusão de Dados. Foram obtidos espectros de Fluorescência Molecular (FM), Ressonância Magnética Nuclear de 1H (RMN de 1H), Infravermelho na região do próximo (FT-NIR) e imagens RGB de um conjunto de 268 amostras de OMI fornecidas pela CELG Distribuição S.A. (CELG D). Posteriormente, foram obtidos espectros de UV-Vis, FM e imagens RGB de um conjunto de 24 amostras degradadas aceleradamente. Modelos PLS foram construídos para prever os parâmetros TI e cor para o conjunto de amostras fornecidas pela CELG D. Para as amostras degradadas aceleradamente modelos PLS foram obtidos para previsão do tempo de degradação. Modelos PARAFAC e MCR-ALS foram construídos com objetivo de avaliar o processo de oxidação do sistema isolante e identificar os espectros puros de possíveis compostos formados durante esse processo. Além disso, Fusão de Dados de baixo nível aliada à seleção de variáveis usando as Variáveis de Importância na Projeção (VIPs) foram utilizadas neste trabalho para a obtenção de modelos PLS. O melhor modelo PLS para o conjunto de amostras fornecidas pela CELG D foi obtido para os dados espectroscópicos fundidos usando seleção de variáveis por VIPS. Esse modelo forneceu um erro médio quadrático de previsão (RMSEP) de 2,9 mN/m para previsão da TI e 0,3 para o parâmetro cor (unidade adimensional). Os modelos PARAFAC e MCR-ALS permitiram avaliar o processo de degradação do sistema isolante. As metodologias propostas são rápidas e não destrutivas, mostrando grandes vantagens sobre os métodos tradicionais, os quais demandam muito tempo, além de gerar uma grande quantidade de resíduos. Este trabalho abre perspectivas para um monitoramento direto (on-line) para avaliar a degradação do sistema isolante, além de ter permitido um estudo mais aprofundado a respeito dos compostos formados durante o processo de degradação.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Vasoaktives Intestinales Peptid (VIP) ist ein 28meres Neuropeptid, das eine Vielzahl biologischer Aktivitäten ausübt, welche durch die Bindung an die heptahelikalen Transmembranrezeptoren VPAC1 und VPAC2 vermittelt werden. VPAC1 ist auf der Oberfläche vieler Tumorzellen überexprimiert. Daher ist VIP gekoppelt mit Signalmolekülen wie z.B. Fluoreszenzfarbstoffen eine interessante Zielstruktur für die optische Detektion von Tumoren. Der Einsatz von VIP in der Tumordiagnostik ist jedoch aufgrund der schnellen proteolytischen Degradation stark limitiert. In der vorliegenden Arbeit wurden VIP-Analoga mit höherer in vitro und in vivo Stabilität identifiziert und synthetisiert. Hierfür wurde eine neue Synthesestrategie entwickelt, welche die hochparallele Herstellung von löslichen VIP-Farbstoff-Konjugaten auf Cellulosemembranen (Spotsynthese) erlaubt. Es wurden 533 N-terminal Carbocyanin-farbstoff-markierte VIP-Analoga synthetisiert, wobei jeder VIP-Rest durch alle übrigen 19 L-Aminosäuren ausgetauscht wurde (Substitutionsanalyse). Alle Analoga wurden mittels Durchflußzytometrie hinsichtlich ihrer Bindung/Internalisierung an VPAC1-überexprimieren-den Zellen getestet. Diese Ergebnisse führten zur Identifizierung von VIP Aminosäureresten, die für die Wechselwirkung mit VPAC1 essentiell sind und lieferten weiterhin Hinweise über eine vorwiegend helikale Struktur des Rezeptor-gebundenen VIPs. Durch Einbau des Farbstoffs an alle VIP-Reste wurden die für die Wechselwirkung mit VPAC1 günstigen Positionen ermittelt. In Kompetitionstudien und cAMP-Assays ausgewählter Farbstoff-markierter VIP-Konjugate wurde demonstriert, daß sowohl die Spezifität als auch die Produktion von cAMP mit Hilfe der Modifikation der Farbstoffposition gesteigert werden konnte. Für die in Rattenleber getestete metabolische Stabilität der VIP-Analoga zeigte die Farbstoffposition nur einen geringen Einfluß. Die metabolische Stabilität konnte jedoch durch eine einzige Modifikation an Position 8 (Asp8 ® Arg8) erhöht werden. Weiterhin wurde das [Arg8]-VIP-Analogon als Kontrastmittel in in vivo Imaging-Experimenten mit VPAC1-überexprimierenden Tumoren inokulierter Mäuse appliziert. Hierbei wurden die Ergebnisse der Stabilitätstests in Rattenleber bestätigt. Das N-terminal farbstoffmarkierte [Arg8]-VIP-Derivat zeigte gegenüber dem nativen N-terminal markierten VIP-Konjugat eine höhere Halbwertszeit in vivo. Darüber hinaus konnte mit [Arg8]-VIP ein höherer Fluoreszenzkontrast zwischen normalen und Tumorgewebe induziert werden. / Vasoactive Intestinal Peptide (VIP) is a 28meric neuropeptide with a broad range of biological activities which are mediated via binding to the heptahelical transmembrane receptors VPAC1 and VPAC2. Since VPAC1 is overexpressed on the surface of numerous tumour cells, VIP coupled with signal structures like fluorescence dyes is an interesting target for the tumour detection in the near-infrared range. The use of VIP in tumour diagnosis is limited due to the rapid proteolytic degradation and therefore suggests need for optimised VIP-analogues with enhanced stability. In the present work a complete substitutional analysis of VIP N-terminally labelled with carbocyanine dyes was performed. For that reason a new synthetic strategy has been developed, which allows the parallel production of soluble VIP-dye conjugates using the spot synthesis technique. The resulting 560 derivatives were tested for binding and internalisation using VPAC1-overexpressing cells by means of flow cytometry. Based on these results a VIP binding motif has been delineated, which facilitates the modification of VIP concerning stability enhancement and preservation of binding characteristics. Using a dye-walk the dye-coupling positions beneficial for cell binding were identified. Radioactive competitions studies and cAMP assays of selected dye-labeled VIP-conjugates demonstrated that specifity as well as production of cAMP or biological activity has been increased by alteration of the dye-position. For increasing metabolic stability of labelled VIP-analogues the dye-position showed little influence. But is has been shown that stability of VIP was increased by only one modification at position 8 (Asp8®Arg8). Furthermore [Arg8]-VIP-derivative has been used as a contrasting agent in in vivo-Imaging experiments with VPAC1-overexpressing tumours inoculated in mice. Here the results of stability test in rat liver extract were confirmed. N-terminally dye-labelled [Arg8]-VIP analogue revealed higher half-life-time in vivo towards N-terminally labelled native VIP-derivative. In addition the [Arg8]-VIP induced a higher tumour contrast between normal and tumour tissue.

Kompaktaggregat är ett integrerat FTX- och FVP-system som används i passivhus. Vi har gjort energiberäkningar i programmet VIP+ för att se hur kompaktaggregatet skulle kunna fungera i passivhus i Sverige. De resultat vi har fått fram ur VIP+ visar att passivhus med ett installerat kompaktaggregat klarar av att nå passivhuskraven ända upp till Skellefteå. De beräknade värdena understiger kraven för passivhus, men en viss marginal behövs för att kraven ska nås även efter uppförandet av byggnaden. Vi har även tolkat tekniska uppgifter på ett sätt som enligt oss har en positiv effekt för passivhus på kallare orter. Därför tror vi inte att värdena för de tre nordligaste platserna är helt tillförlitliga. De övriga plasterna ser bra ut. Passivhus med ett installerat kompaktaggregat klarar enligt oss passivhuskraven i dessa städer; Malmö, Växjö, Göteborg, Stockholm och med tveksamhet Borlänge. / Compact units are an integrated balanced ventilation whit recovery and an extracted air heat pump that are used in passive houses. We have done some energy calculations to see how compact units would be able to operate in Sweden. The results we have been receiving from VIP+, display that a passive house with an installed compact unit manages to achieve the demands for passive houses all the way up to the city Skellefteå. The calculated values are below the demands for passive house, but a certain margin is needed for the demands to be met after the building is complete. We have also construed technical data in a way that can have a positive effect on the passive houses in colder locations. Because of this we do not think the values for the three cities furthest north are entirely reliable. The other locations look good. Passive houses with an installed compact unit would, according to our research meet the passive house demands in these cities; Malmö, Växjö, Göteborg, Stockholm and with a doubt Borlänge.

Ett passivhus är ett hus som i stort sett enbart värms upp av människorna och elapparaterna som finns i huset. Särskilda krav för att få kalla huset för passivhus måste uppfyllas. Vi har gjort en enkätundersökning på tre olika passivhusprojekt för att utreda vad de boende tycker om inomhusklimatet. De utvalda projekten finns i Värnamo, Frillesås och Glumslöv. Enkätsvaren visar att de boende i Frillesås är mycket nöjda, medan mer än 50 % av dem som bor i Glumslöv tycker att det är för varmt på sommaren och för kallt på vintern. För att utreda om de olika konstruktionerna har någon inverkan på inomhusklimatet har beräkningar och simuleringar i datorprogrammen VIP+ och IDA gjorts. Resultaten från de båda programmen visar att vilken av de två konstruktionerna som valts inte bör ha någon påverkan på inomhusklimatet. / A passive house is a house that is mostly heated with energy from humans and from electric devices in the house. Special requirements need to be followed if you want to call the house a passive house. We did a survey on three different passive house projects to investigate the tenants opinion about the indoor climate. The chosen projects are located in Värnamo, Frillesås and Glumslöv. The result of the survey shows that the tenants in Frillesås are very satisfied, while more than 50 % of the tenants in Glumslöv think it’s too hot in the summer and too cold in the winter. To investigate if the construction has any effect on the indoor climate, we did calculations and simulations in the computer programs VIP+ and IDA. The result from both of the programs shows that the chosen construction should not effect the indoor climate.

<p>Kompaktaggregat är ett integrerat FTX- och FVP-system som används i passivhus. Vi har gjort</p><p>energiberäkningar i programmet VIP+ för att se hur kompaktaggregatet skulle kunna fungera i</p><p>passivhus i Sverige.</p><p>De resultat vi har fått fram ur VIP+ visar att passivhus med ett installerat kompaktaggregat klarar</p><p>av att nå passivhuskraven ända upp till Skellefteå. De beräknade värdena understiger kraven för</p><p>passivhus, men en viss marginal behövs för att kraven ska nås även efter uppförandet av</p><p>byggnaden. Vi har även tolkat tekniska uppgifter på ett sätt som enligt oss har en positiv effekt</p><p>för passivhus på kallare orter. Därför tror vi inte att värdena för de tre nordligaste platserna är</p><p>helt tillförlitliga. De övriga plasterna ser bra ut. Passivhus med ett installerat kompaktaggregat</p><p>klarar enligt oss passivhuskraven i dessa städer; Malmö, Växjö, Göteborg, Stockholm och med</p><p>tveksamhet Borlänge.</p> / <p>Compact units are an integrated balanced ventilation whit recovery and an extracted air heat</p><p>pump that are used in passive houses. We have done some energy calculations to see how</p><p>compact units would be able to operate in Sweden.</p><p>The results we have been receiving from VIP+, display that a passive house with an installed</p><p>compact unit manages to achieve the demands for passive houses all the way up to the city</p><p>Skellefteå. The calculated values are below the demands for passive house, but a certain margin</p><p>is needed for the demands to be met after the building is complete. We have also construed</p><p>technical data in a way that can have a positive effect on the passive houses in colder locations.</p><p>Because of this we do not think the values for the three cities furthest north are entirely reliable.</p><p>The other locations look good. Passive houses with an installed compact unit would, according</p><p>to our research meet the passive house demands in these cities; Malmö, Växjö, Göteborg,</p><p>Stockholm and with a doubt Borlänge.</p>

<p> </p><p>Ett passivhus är ett hus som i stort sett enbart värms upp av människorna och elapparaterna som finns i huset. Särskilda krav för att få kalla huset för passivhus måste uppfyllas.</p><p>Vi har gjort en enkätundersökning på tre olika passivhusprojekt för att utreda vad de boende tycker om inomhusklimatet. De utvalda projekten finns i Värnamo, Frillesås och Glumslöv. Enkätsvaren visar att de boende i Frillesås är mycket nöjda, medan mer än 50 % av dem som bor i Glumslöv tycker att det är för varmt på sommaren och för kallt på vintern. <strong></strong>För att utreda om de olika konstruktionerna har någon inverkan på inomhusklimatet har beräkningar och simuleringar i datorprogrammen VIP+ och IDA gjorts. Resultaten från de båda programmen visar att vilken av de två konstruktionerna som valts inte bör ha någon påverkan på inomhusklimatet.</p><p> </p> / <p><p>A passive house is a house that is mostly heated with energy from humans and from electric devices in the house. Special requirements need to be followed if you want to call the house a passive house.<strong></strong></p><p>We did a survey on three different passive house projects to investigate the tenants opinion about the indoor climate. The chosen projects are located in Värnamo, Frillesås and Glumslöv. The result of the survey shows that the tenants in Frillesås are very satisfied, while more than 50 % of the tenants in Glumslöv think it’s too hot in the summer and too cold in the winter.</p>To investigate if the construction has any effect on the indoor climate, we did calculations and simulations in the computer programs VIP+ and IDA. The result from both of the programs shows that the chosen construction should not effect the indoor climate.</p>

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Det finns idag många byggnader i Sverige som uppfördes under en tid då energianvändningen inte ansågs vara ett problem och därmed inte har haft samma krav på energiprestanda som vi har idag. Vid renovering är det därför viktigt att tänka på energieffektiva lösningar. Studier visar att det går att sänka energiförbrukningen med 36-54 % genom renovering, dock är det inte alltid lönsamt med vissa åtgärder enligt LCC-analyser.Studien som gjorts genom modellering och platsbesök på ett flerbostadshus med allt för hög energianvändning har haft som mål att sänka denna genom förslag på åtgärder. Resultaten visar att installation av värmeåtervinning och förbättring av klimatskalet har stor effekt på husets energiprestanda. Det har också framgått att boendes inverkan på energibalansen kan vara svår att uppskatta, vilket orsakat svårighet i att teoretiskt få samma energiförbrukning som energideklarationen visar.De förslag som rekommenderas är installation av värmeåtervinning, solceller, individuell mätning och debitering (IMD) samt tätning av fönster/dörr då dessa enligt LCC-analysen är lönsamma. Med nämnda åtgärder har energianvändningen sänkts från 173 till 114 kWh/m2 och år, en sänkning med 34 %. / There are today many buildings in Sweden that were built during a time when energy use was not considered a problem and thus did not have the same energy performance requirements as we have today. Therefore, in renovation, it is important to consider energy-efficient solutions. Studies show that it is possible to reduce the energy consumption of a building by 36-54 % through renovations, however, it is not always profitable when using a LCC-analysis.The study conducted by modeling and site visits to a multi-family housing with excessive energy use has been designed to reduce this through proposed measures. The results show that the installation of heat recovery and improvement of the building envelope have a major effect on the energy performance of the house. It has also been observed that the resident’s impact on the energy balance can be difficult to estimate, in which the study had problems to theoretically gaining the same energy consumption as the energy declaration shows.The proposals that are recommended are installation of heat recovery, solar cells, individual measurement and billing (IMD) and window/door seals as these are profitable according to the LCC analysis. With proposed measures, energy consumption has been reduced from 173 to 114 kWh/m2 per year, a reduction of 34 %.