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An evaluation of the Accelerate Christian Schools for reaching children for the Kingdom of God as part of Missio Dei in South Africa / Jones D.J.C.Jones, Dina Johanna Christina. January 2011 (has links)
Secularist views are a challenge to the field of religious education. Their worldview and influence in
society will be discussed. This study evaluates the theocratic model under the apartheid regime, the
co–operative model and the religion policy under the new democratic government.
The areas that the researcher investigated in this study are centred on the effectiveness of the mission
calling of the School of Tomorrow, Accelerated Christian Education. In order to achieve this
outcome, the history of ACE Schools in America and South Africa will be discussed, as well as the
role of the school, the parent and the teacher in missio Dei.
An analysis and evaluation will be done on Christian educators such as Martin Luther, John Calvin
and John Knox, as well as educational deform under Hitler.
A study will be done on how Biblical doctrine was formed in children’s lives in the Old and New
Testament. / Thesis (M.A. (Missiology))--North-West University, Potchefstroom Campus, 2012.
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Bloqueio do sistema renina-angiotensina atenua lesões em órgãos-alvo em modelo de diabetes mellitus tipo 2 e hipercolesterolemia induzidos por dieta / Blockade of renin-angiotensin system attenuates target-organ lesions in a model of type 2 diabetes mellitus and hypercholesterolemia induced by dietHelfenstein, Tatiana [UNIFESP] January 2009 (has links) (PDF)
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Previous issue date: 2009 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Com o crescente aumento da prevalência mundial de diabetes mellitus, tem-se buscado modelos
experimentais para melhor compreensão de sua fisiopatologia e tratamento que atendam de maneira
mais adequada à preservação de células beta, proteção de órgãos-alvo e atenuação da aterosclerose.
Objetivos: Desenvolver modelo experimental de diabetes mellitus tipo 2 induzido por meio de dieta, e
utilizá-lo para examinar os efeitos de um inibidor da enzima conversora de angiotensina (IECA) e de um
bloqueador do receptor de angiotensina (BRA) na proteção de órgãos-alvo. Métodos: Coelhos machos
Nova Zelândia (n=49) receberam dieta acrescida de banha (10%), sacarose (40%) durante todo o
protocolo do estudo além de colesterol (0,5% nos três primeiros meses e 0,1% nos meses
subseqüentes). Os animais receberam aleatoriamente: apenas a dieta sem fármacos (G1), olmesartana
5 mg (G2), quinapril 30 mg (G3), ou a combinação de ambos (G4), acrescidos à mesma dieta por seis
meses. Foram analisados lípides, frutosamina, glicose e insulina em jejum com cálculo dos índices para
resistência à insulina e função de células beta pancreáticas. Foram ainda examinadas as áreas sob as
curvas de insulina e glicose, após infusão de glicose intraperitoneal. Angiofluoresceinografias e análises
histopatológicas avaliaram lesões em órgãos-alvo. Resultados: Os coelhos ganharam peso, e houve
aumento dos níveis de glicose, colesterol total, LDL-C e triglicérides e redução do HDL-C (p <0,05 vs.
basal). A frutosamina e o HOMA-IR se elevaram, enquanto houve redução do HOMA-β (p <0,05 vs.
basal). Sinais precoces de retinopatia diabética foram observados a partir do terceiro mês, progredindo
até o final do experimento (p<0,0005). Lesões ateroscleróticas em aorta, esteatofibrose hepática e
infiltrado glomerular de macrófagos constituíram os principais achados histomorfológicos. O bloqueio do
sistema renina-angiotensina modificou favoravelmente a glicemia e o HOMA-β (p<0,05) e houve
atenuação do número e grau dos microaneurismas pelo tratamento com BRA isoladamente ou
combinado com IECA (p<0,05 vs. G1). Conclusões: Nosso modelo reproduziu várias características
glucometabólicas do diabetes mellitus tipo 2 humanóide, incluindo déficit de secreção e resistência à
insulina. O bloqueio do sistema renina-angiotensina atenuou algumas alterações bioquímicas e as lesões
microvasculares em retina. / With the increasing prevalence of diabetes mellitus worldwide, new experimental models are required to
better understand the pathophysiology of this disease and to offer therapeutic options that can preserve
pancreatic beta-cells, protect target organs and attenuate atherosclerosis. Objective: The aims of this
study were to develop an experimental model of type 2 diabetes mellitus induced by diet and assess on
this model the effects of an angiotensin-converting enzyme inhibitor (ACEI) and an antagonist of the
angiotensin II type1 receptor (AT1R) on target organ protection. Methods: New Zealand male white rabbits (n=49) were fed high-fat/high-sucrose (10/40%) during the study protocol and cholesterol-enriched
diet (0.5% in the first three months followed by 0.1% until the end of the study). These animals were
randomized to receive: diet alone (G1), olmesartan 5 mg (G2), quinapril 30mg (G3), or combination of
both drugs (G4), added to the same diet for six months. Fasting lipids, fructosamine, glucose and insulin,
with calculation of insulin resistance and beta-cell function indexes were evaluated. The areas under the
curves for glucose and insulin were obtained after intraperitoneal glucose bolus injection. Fluorescein
angiography and histopathological analyses were performed to assess target-organs lesions. Results:
The animals gained weight, and there were increases in blood glucose, total cholesterol, LDL-C and
triglycerides, and decrease in HDL-C (p<0.05 vs. baseline). Fructosamine levels and the homeostasis
model assessment of insulin resistance (HOMA-IR) were increased, while there was a reduction in the
HOMA-β (p<0.05 vs. baseline). Early clinical features of diabetic retinopathy were seen since the third
month, progressing up to the end of the experiment (p<0.0005). Aortic atherosclerosis, hepatic
steatofibrosis and glomerular macrophage infiltration were the main histomorphologic findings of this
study. The renin-angiotensin system (RAS) blockade favorably modified blood glucose and the HOMA- β
(p<0.05) and promoted attenuation of the number and grade of microaneurysms in retina in the group of
animals receiving AT1R antagonist or combined therapy with the ACEI (p<0.05 vs. G1). Conclusion: Our
model reproduced several glucometabolic characteristics of humanoid type 2 diabetes, including
decreased insulin secretion and insulin resistance. The RAS blockade attenuated some biochemical
abnormalities and the diabetic retinopathy. / FAPESP: 07/51058-8
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Adolescentes em situação de acolhimento institucional prolongado : análise do processo de desligamentoBenetti, Daniella Simões 14 December 2012 (has links)
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Previous issue date: 2012-12-14 / The host institution is one of the lines of political action to guarantee the rights of children and adolescents, whose character is provisional and exceptional form of transition to reintegration and family or for integration in a foster family. Usually occurs when families are considered unable to provide child care, or when they have behaviors that put at risk the full development of children. Both the removal of the child or adolescent from family and his dismissal of the institution are made by the Government, however, there is a lack of effective public policies and scientific research data that cover the issue of institutional shutdown, more specifically. The present study aimed to: a) describe the process of institutional shutdown according to the opinion of adolescents who lived in a situation of institutional care for an extended period and the leaders; b) describe the operation of a host institution. This was a qualitative, exploratory. The study included two leaders of the two institutions reception and six individuals who remained in a state of Reception beyond the time allowed by law, and who completed two years of majority, including motive, which led to the shutdown. The instruments used for data collection were: Interview Script 1) The characteristics of the host institution and Interview Script 2) Shutdown Institutional, both were applied with the leaders of institutions and even Home, Interview Script 3) The Consequences Off the Institutional conducted with former accepted. The questions of the interview scripts were submitted before the application, the judges pointed suggestions to better understand the context. The application of the interviews took place at different times and places previously chosen by the participants. By means of the instruments were performed quantitative analyzes, obtained from the defined categories, with the main parameter to content analysis. The results indicated that in some respects the institutions surveyed described themselves differently, therefore, had different types of conduct with regard to the implementation of some of its functions, humanization of the shutdown process, preparation gradual for that time, maintenance of family ties, family support for restructuring, encouraging interaction with other families, participation in the services offered by the local community. Other aspects described by the Institutional leaders have been characterized equally and refer to similar residential maintenance of emotional bonds between siblings, attendance at small groups, among others. Most adolescents showed that the shutdown was a moment Institutional permeated by feelings of sadness, fear, insecurity, memories, and the individual is ready or not to experience this moment and its consequences were related to the difficulty in getting a job, live alone, keep themselves financially. It is concluded that there is need for specific policies that cover the issue of Institutional shutdown, to support the teen financially, emotionally, socially, before, during and after this procedure. Still, training for all social actors involved in institutional care situation for better care and understanding of the context. / O acolhimento institucional é uma das linhas de ação política da garantia dos direitos da criança e do adolescente, cujo caráter é provisório e excepcional; forma de transição para reintegração familiar e ou para integração em família substituta. Normalmente ocorre quando as famílias são consideradas sem condições de dar assistência aos filhos, ou seja, quando apresentam comportamentos que colocam em risco o desenvolvimento pleno dos filhos. Tanto o afastamento da criança ou adolescente do convívio familiar quanto o seu desligamento da instituição são realizados pelo Poder Público, porém, observa-se a inexistência de políticas públicas eficazes, bem como dados de pesquisas científicas que abarquem a questão do desligamento institucional, mais especificamente neste estudo. O presente estudo teve como objetivos: a) descrever o processo de desligamento institucional sob a óptica de adolescentes que viveram em situação de acolhimento institucional por um período prolongado e das dirigentes; b) descrever o funcionamento de uma instituição de acolhimento sob a óptica do dirigente da instituição. Tratou-se de uma pesquisa qualitativa, de caráter exploratório. Participaram do estudo duas dirigentes de duas Instituições de Acolhimento e seis indivíduos que permaneceram em situação de acolhimento além do tempo permitido por lei, dois anos e que completaram maioridade, motivo inclusive, que acarretou o desligamento. Os instrumentos utilizados para coleta de dados foram: Roteiro de Entrevista 1) As características da Instituição de Acolhimento e Roteiro de Entrevista 2) O Desligamento Institucional, ambos foram aplicados com os dirigentes das Instituições de Acolhimento e ainda, Roteiro de Entrevista 3) As Conseqüências do Desligamento Institucional realizado com os ex-acolhidos. As perguntas dos roteiros de entrevista foram submetidas, antes da sua aplicação, a juizes que apontaram sugestões para melhor compreensão do contexto. A aplicação das entrevistas ocorreu em momentos distintos e em locais previamente escolhidos pelos participantes. Por intermédio dos instrumentos foram realizadas análises quantitativas, obtidas a partir das categorias definidas, tendo como parâmetro principal a análise de conteúdo. Os resultados indicaram que em alguns aspectos as Instituições pesquisadas se descreveram de forma diferente, pois, apresentaram diferentes tipos de condutas no que se refere à execução de algumas de suas funções, entre estas, humanização do processo de desligamento, preparação gradativa para o referido momento, manutenção dos vínculos familiares, apoio à reestruturação familiar, incentivo à convivência com outras famílias, participação nos serviços oferecidos pela comunidade local. Outros aspectos institucionais descritos pelas dirigentes, se caracterizaram de forma igualitária e se referem à semelhança residencial, manutenção dos vínculos afetivos entre irmãos, atendimento em pequenos grupos, entre outros. A maioria dos adolescentes apontou que o desligamento institucional foi um momento permeado por sentimento de tristeza, medo, insegurança, lembranças, tendo o individuo se preparado ou não para vivenciar este momento e suas conseqüências estavam relacionadas à dificuldade em arrumar emprego, viver sozinho, manter-se financeiramente. Conclui-se que há necessidade de políticas públicas especificas que abarquem a questão do desligamento Institucional, que ampare o adolescente financeiramente, emocionalmente, socialmente, antes, durante e após o referido processo. Indica-se ainda, a necessidade de capacitação para todos os atores sociais envolvidos com situação de acolhimento Institucional para melhor atuação e compreensão do contexto.
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BIOACTIVE AND ALLERGENIC PROPERTIES OF EDIBLE CRICKET (GRYLLODES SIGILLATUS) PEPTIDESFelicia G Hall (9739430) 10 December 2020 (has links)
<p>Cardiovascular diseases (CVD) and their risk factors remain the leading cause of
morbidity and mortality in North America. Food-derived
bioactive peptides (BAP) have been shown to play a role in regulating
physiological pathways of CVD risk factors including hypertension, diabetes,
and chronic inflammation. Common sources of BAP include dairy and plant
proteins. In addition to being an alternative protein
source, it is now accepted that edible insect proteins can also confer
health benefits beyond nutrition. However, as with any novel protein source,
allergenicity remains a major concern surrounding edible insect consumption. </p>
<p>This
dissertation aimed to: 1) Evaluate the bioactive potential of peptides from an
edible cricket species and; 2) determine the effects of BAP production methods
on immunoreactivity. First, peptide-rich extracts were generated from farmed
food-grade crickets via enzymatic hydrolysis techniques with the commercial
protease Alcalase™. To measure the <i>in
vitro</i> bioavailability, cricket peptides were also subject to simulated
gastrointestinal digestion (SGD). Peptides and their digests were tested for
relevant bioactivities and active groups were further fractionated by
chromatographic methods to identify the major peptides responsible for the
bioactivity. When tested for <i>in vitro</i>
antihypertensive and anti-glycemic properties, cricket peptides were found to
inhibit the activities of angiotensin converting enzyme, dipeptidyl
peptidase-4, α-amylase, and α-glucosidase. The anti-inflammatory potential was
expounded using RAW-264.7 macrophages and human umbilical vein endothelial
cells (HUVEC). Cricket peptides (after SGD) effectively lowered NF-κB, MCP-1,
and IL-6 production in cells without affecting their viability. Proteomic
analyses identified 18 sequences from the enriched cationic peptide fraction
that showed the highest activity. Three novel peptides were identified via
molecular docking, as potent ACE-inhibitors and binding was similar to that of
the commercial drug captopril. Key binding characteristics included interaction
with hydrophobic amino acids (Phe, Pro, Leu) near the C-terminal position and
coordination with Zn (II) ions near the ACE active site.</p>
<p>Immunological
reactivity was measured by IgE-binding from shrimp-allergenic patient sera to
antigens present within cricket peptides. Our studies demonstrate that
immunoreactivity was impacted by enzymatic hydrolysis, depending on the
conditions and heating source used. Tropomyosin (a major shrimp allergen) was
extracted from both untreated crickets and protein hydrolysates, and verified
as the major reactive protein. Tropomyosin reactivity decreased (under both
partial and extensive hydrolysis) or retained (under conditions which prevented
epitope cleavage). However, using microwave-assisted enzymatic hydrolysis was
effective at decreasing tropomyosin reactivity in all immunoassays tested (IgG
and IgE). Proteomic and immunoinformatic analyses revealed prominent allergen
binding regions of cricket tropomyosin available for cleavage during enzymatic
hydrolysis. Conserved antigen regions showed greater homology with other
crustacean species, but not with other well studied allergenic insect proteins
(i.e., cockroach). Lastly, LC-MS/MS and FT-Raman spectrometry suggests that
reactivity was affected due to distinct epitope cleavage within the protein
instead of decreased antigen extractability/solubility. </p>
<p>The
findings of this dissertation support that edible cricket proteins are a
potential source of bioactive peptides for functional food or nutraceutical
development. Additionally, using protein extraction methods such as
microwave-assisted hydrolysis seems a promising tool for minimizing the
immunoreactivity of the allergen present in this edible cricket species.</p>
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Implementation and Sustainability of Trauma-Informed Care Via Multi-Tiered System of Support (MTSS)Brice, Aisha Larie Elloie 01 January 2022 (has links)
Trauma-informed care is a relatively new construct in K-12 education, and districts across the United States are seeking avenues to meet the needs of their students. The COVID-19 pandemic elevated the need for districts to develop appropriate supports to address student and adult trauma. Traumatic events can affect a child’s mental, physical, social-emotional, and/or spiritual well-being (Substance Abuse and Mental Health Service Administration, 2011). Furthermore, children with an elevated risk of trauma may not be able to access these supports outside of the school setting (Baweja et al., 2015). Therefore, educational systems need to develop trauma-informed care models for schools that furnish a sense of safety and community so that students receive the necessary support. This evaluation aimed to identify how the moderating factors of district administrators, site administrators, climate and culture, and teacher capacity affect trauma-informed care via a multi-tiered system of support (MTSS) framework. It included a specific focus on Tier 1, universal access, to improve timely support for students.
This formative program evaluation explored the impact of the moderating factors through the viewpoint of a newly created conceptual framework. I used qualitative and quantitative data sources to explore the multiple aspects of the phenomenon. The ARTIC-45 provided descriptive statistics about educators’ attitudes toward trauma-informed care. The observational data and analysis of the Panorama Education social-emotional learning (SEL) survey that assessed 3rd-12th students social-emotional well-being provided character to the evaluation.
The data analysis yielded inconsistent results. The observational data strongly indicated a trauma-informed environment in which students were given clear expectations and engaged in positive reciprocal interactions with peers and adults. The ARTIC-45 data showed that administrators, teachers, and support staff responded favorably to trauma-informed care approaches. Though statistical significance could not be obtained due to the sample size constraint of being too small, the data provided context to the other data sources. For example, teacher capacity could not be quantified, but the data provided context to the overall staff capacity. The review of the archival and current SEL survey data showed that favorability among domains varied by site and grade level. The variations in students' social-emotional favorability can be related to their lived experiences and it is important to track over time to monitor how experiences, expressions, and feelings change over time. Students' perspectives regarding themselves and their environment differed from the observational data. This indicates that educators should not solely rely on observational data to determine students’ social-emotional well-being. Furthermore, an SEL survey can be used as a tool to understand students’ well-being and thus provide them with timely support.
The evaluation determined that district administration, site administration, climate and culture and staff capacity can positively impact a multi-tiered, trauma-informed care environment. In these settings, student behavior and social-emotional well-being is viewed in a healing-centered manner. Districts can create a multi-tiered, trauma-sensitive culture and provide support to enhance teachers’ capacity to implement trauma-informed care, take advantage of administrative influence, develop community partnerships, and create a culture that is open to systematic change.
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New Advances in High-quality Screening by Capillary Electrophoresis: A Unified Platform for Thermodynamic and Kinetic Characterization of Protein-Small Molecule Interactions / High-quality Screening by Capillary ElectrophoresisGavina, Jennilee 12 1900 (has links)
<p> The development of high-quality screening assays for the identification of biologically active ligands is critical in drug discovery. This thesis is aimed at developing new advances m capillary electrophoresis (CE) for the characterization of the conformational stability and enzymatic activity of protein targets with small molecules. CE provides a convenient platform for unbiased assessment of multiple thermodynamic and kinetic parameters associated with biomolecular interactions involving regulatory protein or isomerase enzymes, where various sample pretreatment steps can be integrated directly in-capillary during analysis. The first two chapters of the thesis (Chapters II, III) outline the development of dynamic ligand exchange-affinity capillary electrophoresis (DLEACE) as a novel strategy for the screening of allosteric ligands based on the differential stability of urea-induced unfolding of various apolholo-protein states of cAMP receptor protein constructs. This work introduced a label-free and multivariate approach for ligand selection based on complementary thermodynamic parameters that allowed for determination of the dissociation constant of protein-ligand interactions over a wide dynamic range (> 10^4, Kd = nM-mM). The subsequent two chapters of the thesis (Chapters IV, V) describe the development of a novel kinetic assay for unbiased characterization of isomerase activity associated with D/L-amino acid metabolism using hydroxyproline epimerase as a model system. Stereoselective resolution of various hydroxyproline isomers was accomplished via off-line or on-line chemical derivatization with dynamic complexation usmg chiral selector(s) in order to screen potential inhibitors for putative epimerase and racemase activity. The integration of both thermodynamic and kinetic strategies for differentiation of mutant from wild-type enzymes was important for revealing the function of a catalytic acid/base cysteine pair in the epimerase active site. Overall, this thesis outlines an integrative framework based on CE for high-quality screening, which is relevant in reducing the high attrition rate of lead candidates in drug development. </p> / Thesis / Doctor of Philosophy (PhD)
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Cross-Reactivity of IgG Antibodies and Virus Neutralization in mRNAVaccinated People Against Wild- Type SARS-CoV-2 and the Five Most Common SARS-CoV-2 Variants of ConcernSchwarze, Mandy, Krizsan, Andor, Brakel, Alexandra, Pohl, Fabian, Volke, Daniela, Hoffmann, Ralf 11 July 2023 (has links)
The rapid development, approval, and production of vaccines against the severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) in less than 1 year after the first reports
of a new infectious disease was a real game changer, providing 80%–90% efficacy in
preventing severe etiopathologies of the coronavirus disease 2019 (COVID-19). These
vaccines induce an immune response against the SARS-CoV-2 spike (S) protein located
on the surface of the virus particle. Antibodies (Abs) recognizing the S-protein can inhibit
binding of the virus via the S-protein to the angiotensin-converting enzyme-2 (ACE-2)
receptor expressed on different human cells, especially when these Abs bind to the
interaction site, the so-called receptor-binding domain (RBD). We have expressed the
RBDs of wild-type SARS-CoV-2 and five variants of concern (VOCs) to test the immune
response in people before vaccination with mRNA vaccines BNT162b2 and mRNA-1273
and after up to three vaccinations using in-house ELISA and inhibition assays. The
methods of both assays are provided. Both vaccines initiated similarly high IgG titers after
two vaccinations against the wild-type and even two VOC-RBDs (alpha and delta) and
strongly inhibited the corresponding RBD-ACE-2 binding. The IgG titers and inhibition of
ACE-2 binding were lower for beta and gamma RBDs and much lower for omicron RBD.
The third vaccination after 6 months strongly increased both the IgG titers and the
neutralizing effect against all variants, especially for omicron, leading to 63% ± 13%
neutralization potential. Importantly, neutralization linearly increased with the IgG titers.
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Prävention des Nierenversagens und der Nierenfibrose bei hereditären Erkrankungen der glomerulären Basalmembran (Alport-Syndrom) bei COL4A3-Knockout-Mäusen mit dem Reninantagonisten Aliskiren / Prevention of renal failure and renal fibrosis in hereditary diseases of glomerular basement membrane (Alport-Syndrome) in COL4A3 knockout mice with Aliskiren a direct renin inhibitorTheisen, Stephanie 04 June 2012 (has links)
No description available.
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Adverse Childhood Experiences and Couple Relationships: Impacts on Relationship Quality and Partner SelectionRedd, Michael John January 2017 (has links)
No description available.
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Intravital imaging of hemodynamic glomerular effects of enalapril or/and empagliflozin in STZ-diabetic miceKroeger, Hannah, Kessel, Friederike, Sradnick, Jan, Todorov, Vladimir, Gembardt, Florian, Hugo, Christian 30 May 2024 (has links)
Background: Diabetic kidney disease is the leading cause of end-stage renal disease. Administration of ACE inhibitors or/and SGLT2 inhibitors show renoprotective effects in diabetic and other kidney diseases. The underlying renoprotective mechanisms of SGLT2 inhibition, especially in combination with ACE inhibition, are incompletely understood. We used longitudinal intravital microscopy to directly elucidate glomerular hemodynamics on a single nephron level in response to the ACE inhibitor enalapril or/and the SGLT2 inhibitor empagliflozin.
Methods: Five weeks after the induction of diabetes by streptozotocin, male C57BL/6 mice were treated with enalapril, empagliflozin, enalapril/empagliflozin or placebo for 3 days. To identify hemodynamic regulation mechanisms, longitudinal intravital multiphoton microscopy was employed to measure single nephron glomerular filtration rate (snGFR) and afferent/efferent arteriole width.
Results: Diabetic mice presented a significant hyperfiltration. Compared to placebo treatment, snGFR was reduced in response to enalapril, empagliflozin, or enalapril/empagliflozin administration under diabetic conditions. While enalapril treatment caused significant dilation of the efferent arteriole (12.55 ± 1.46 µm vs. control 11.92 ± 1.04 µm, p < 0.05), empagliflozin led to a decreased afferent arteriole diameter (11.19 ± 2.55 µm vs. control 12.35 ± 1.32 µm, p < 0.05) in diabetic mice. Unexpectedly under diabetic conditions, the combined treatment with enalapril/empagliflozin had no effects on both afferent and efferent arteriole diameter change.
Conclusion: SGLT2 inhibition, besides ACE inhibition, is an essential hemodynamic regulator of glomerular filtration during diabetes mellitus. Nevertheless, additional mechanisms—independent from hemodynamic regulation—are involved in the nephroprotective effects especially of the combination therapy and should be further explored in future studies.
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