Space-Time Adaptive Processing (STAP) has been widely used in spaceborne and airborne radar platforms in order to track ground moving targets. Jammer is an hostile electronic countermeasure that is being used to degrade radar detection and tracking performance. STAP adapts radar&rsquo / s antenna radiating pattern in order to reduce jamming effectiveness. Jamming power that enters the system is decreased with respect to the adapted radiation pattern. In this thesis, a generic STAP radar model is developed and implemented in simulation environment. The implemented radar model demonstrates that, STAP can be used in order to suppress wideband jammer effectiveness together with ground clutter effects.

Autoinducer 2 (AI-2) is a quorum sensing signal employed by bacteria to coordinate their response to environmental stresses. The objective of this study was to determine the relationship between presence of AI-2 molecules, exposure to sub-therapeutic tetracycline, the expression of genes associated with the conjugal transfer of antibiotic resistance plasmids, and the conjugal transfer of these plasmids in Escherichia coli. The studies showed that AI-2 activity increased in Tets E. coli in the presence of tetracycline (2 µg/mL) under both batch and continuous culture conditions. The presence of AI-2 molecules induced tetracycline tolerance development in Tets E. coli. The studies showed that the survival rates of Tets E. coli exposed to AI-2 molecules were significantly higher compared to the cells not exposed to AI-2 molecules or cells that were exposed to only LB (Lauria-Bertani) broth. Molecular analyses using real-time PCR indicate that the expression of at least one conjugation-associated gene (trbC) is increased 9-fold in cells exposed to AI-2 molecules in the presence of sub-therapeutic tetracycline compared to its negative controls. The transconjugation frequency of the plasmid RP4 carrying the tet(A) gene increased between 10-100 fold in the presence of AI-2 molecules. In companion studies, AI-2-like activity was detected in fish, tomatoes, cantaloupes, carrots and milk samples. Interestingly, ground beef and poultry meat contained substances that appear to inhibit AI-2 activity. Collectively, these results highlight the potential importance of bacterial quorum sensing signals such as AI-2 in the response of bacterial cells to environmental stimuli and the possible role of quorum sensing signals in the quality and safety of foods.

In this thesis, it is shown how aflatoxins can be determined in biological material. The thesis is a summary of five papers. Aflatoxins are carcinogenic mycotoxins produced by Aspergillus moulds. Methods were developed for the determination of aflatoxins in samples of airborne dust and human urine collected at feed factories. For the dust samples from such agricultural products as copra, cotton seed and maize, methods were developed for the determination of aflatoxins B1, B2, G1 and G2. For urine samples, methods were developed for analysing the four aflatoxins above that naturally occur in dust, and the metabolites aflatoxins M1 and Q1. Sample preparation of dust samples included solvent extraction, filtration and immunoaffinity column extraction. Urine samples were cleaned up using immunoaffinity column extraction or solid-phase extraction using ethyl bonded-phase columns. All extractions with these columns were automated by means of a laboratory robot. Reversed-phase liquid chromatography was used to separate the aflatoxins in the cleaned-up extracts. Detection was performed by fluorescence after post-column derivatization by addition of bromine. Parameters for the derivatization were studied using factorial designs. To confirm the identity of aflatoxins in naturally contaminated airborne dust samples and spiked urine, liquid chromatography was combined with electrospray mass spectrometry. The detection limits of the aflatoxins in dust samples were in the range 1.8-3.1 ng/g in 10-mg dust samples using fluorescence detection. Aflatoxins were determined in spiked urine down to the 6.8-18 pg/ml level. In naturally contaminated dust of copra and cotton seed, aflatoxins were detected with a content of 9-50 pg/mg of aflatoxin Bi. No aflatoxins could be detected in any urine sample obtained from feed factory workers that were less than 6.8 pg/ml of aflatoxins B1, B2, G1 and G2 and less than 18 pg/ml of aflatoxins M1 and Q1. / <p>Diss. (sammanfattning) Umeå : Univ., härtill 5 uppsatser</p> / digitalisering@umu

The iron ore mining industry makes use of various processes that result in the release of airborne dust into the surrounding atmosphere where workers are exposed, to produce a final product. The deposition in the lung and toxicological influences of airborne dust can be determined by their physical- and chemical characteristics. The Occupational Health and Safety Act (OHSA) regulations for hazardous chemical substances have no current system of how the physical- and chemical properties of particulates originating from specific areas will influence a worker‘s exposure and health, especially for ultrafine particles (UFP). It is therefore imperative to characterise airborne dust containing micrometer and UFP size particles originating from specific areas to determine if there are physical- and chemical characteristics that may or may not have an influence on the workers‘ health. Aim: This pilot study is aimed at the physical- and chemical characterisation of the airborne iron ore dust generated at the process areas of an opencast iron ore mine. Method: Sampled areas included the Primary-secondary crusher, Tertiary crusher, Quaternary crusher and Sifting house. Gravimetric sampling was conducted through the use of static inhalable- and respirable samplers in conjunction with optical- and condensation particle counters that were placed near airborne dust- emitting sources. Physical- and chemical characterisation was done with the use of scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). Results: The results found in the study indicate high mass concentration levels of inhalable dust at all four process areas, as well as high levels of respirable dust found at the primary- secondary crusher area. Particle size distribution optical particle counter (OPC) results indicate that the majority of particles at all four process areas are in the region of 0.3 μm in size. Condensation particle counter (CPC) results integrated with OPC results indicate that at the primarysecondary and Tertiary crushers the majority of particles are found to be in the size fraction <0.3 μm. SEM analysis indicates that particle agglomeration largely occurs in the airborne iron ore dust. Particle splinters originating from larger particle collisions and breakages are present in the airborne dust. EDS analysis indicates that the elemental majority of the airborne iron ore dust consists of iron, oxygen, carbon, aluminium, silicon, potassium and calcium. The elemental percentages differ from each process area where an increase in iron and decrease in impurities can be seen as the ore moves through the beneficiation process from the Primary-secondary crusher to the Sifting house. Conclusion: The results obtained from the physical- and chemical properties of the airborne iron ore dust indicate high risk of over-exposure to the respiratory system, as well as possible ultrafine particle systemic exposure, that may overwhelm the physiological defense mechanisms of the human body and lead to reactive oxygen species (ROS) formation and the development of pathologies such as siderosis, silicasiderosis and lung cancer. / MSc (Occupational Hygiene), North-West University, Potchefstroom Campus, 2014

The aims and objectives: The aims and objectives of this study were to characterize and compare the airborne particulate matter in the tankhouse and crusher areas of a base metal refinery sampled with two separate methods, in terms of mass concentration, nickel content, and particle size distribution. Methods: Area sampling was conducted in the two areas. Two methods were applied to collect particulate samples. The first is a multi–stage virtual impactor, the Respicon, which was used to determine the three critical particle fractions (inhalable, thoracic and respirable). The NIOSH 7300 method determined the particle concentration and nickel percentage present in each fraction. Using formulas provided by the manufacturers two additional particle–size fractions (extra–thoracic and trachea–bronchial) could be calculated. The second was based on the standard NIOSH 0500 method, which determined particle size distribution depicted as cumulative percentages. The samples were analyzed using laser scattering instrumentation. Results: In the tankhouse the highest level of exposure was to particles bigger than 10 um, with the highest nickel percentage also falling into this range. However, high nickel percentages were present in all three cut–off sizes (4 um, 10 um and > 10 um). The particle concentration for the crusher area was the highest for particulates bigger than 10 um, with the highest nickel percentage present in this fraction. After comparing the tankhouse and crusher areas, it is clear that the particle concentration is much higher in the crusher area according to all sampling methods used. The nickel content present in the analysis of these areas is of great concern. Conclusion: With the knowledge obtained through this research one hopes to establish a basis for particle size sampling in the platinum mining industry. This may lead to the development of health based OEL's and reflect a more accurate evaluation of workers particulate exposure. This information will give a greater understanding of health risks workers are exposed to. / Thesis (M.Sc. (Occupational Hygiene))--North-West University, Potchefstroom Campus, 2011.

The aims and objectives: The aims and objectives of this study were to characterize and compare the airborne particulate matter in the tankhouse and crusher areas of a base metal refinery sampled with two separate methods, in terms of mass concentration, nickel content, and particle size distribution. Methods: Area sampling was conducted in the two areas. Two methods were applied to collect particulate samples. The first is a multi–stage virtual impactor, the Respicon, which was used to determine the three critical particle fractions (inhalable, thoracic and respirable). The NIOSH 7300 method determined the particle concentration and nickel percentage present in each fraction. Using formulas provided by the manufacturers two additional particle–size fractions (extra–thoracic and trachea–bronchial) could be calculated. The second was based on the standard NIOSH 0500 method, which determined particle size distribution depicted as cumulative percentages. The samples were analyzed using laser scattering instrumentation. Results: In the tankhouse the highest level of exposure was to particles bigger than 10 um, with the highest nickel percentage also falling into this range. However, high nickel percentages were present in all three cut–off sizes (4 um, 10 um and > 10 um). The particle concentration for the crusher area was the highest for particulates bigger than 10 um, with the highest nickel percentage present in this fraction. After comparing the tankhouse and crusher areas, it is clear that the particle concentration is much higher in the crusher area according to all sampling methods used. The nickel content present in the analysis of these areas is of great concern. Conclusion: With the knowledge obtained through this research one hopes to establish a basis for particle size sampling in the platinum mining industry. This may lead to the development of health based OEL's and reflect a more accurate evaluation of workers particulate exposure. This information will give a greater understanding of health risks workers are exposed to. / Thesis (M.Sc. (Occupational Hygiene))--North-West University, Potchefstroom Campus, 2011.

The sound insulation demands for dwellings and public building has increased over the years as the number of sound sources has grown. From the outside our homes are exposed to noise from cars, trains, airplanes, etc. Noise intrudes from our neighbours and their television and stereo equipments. Also noise from spaces for mechanical services systems tends to become more important due to increasing energy saving demands. This thesis presents new analytical models for predicting the sound reduction index of single or double plate systems. In the single plate case, a theoretical and experimental analysis of the air-borne sound transmission through a single plate is presented. The plate is assumed to be excited by a diffuse sound field and the velocity distribution of the plate is derived from the Kirchoff plate equation in the frequency domain. The resulting Fourier transform is evaluated using residue calculus and the solution is verified numerically. The analytical model is valid for a wide frequency range, both below, above and at the critical frequency. Special interest is paid to the area dependency of the sound reduction index. This technique is further expanded for the double plate case by adding another plate and an intermediate layer which is modelled as a locally reacting spring. The model is valid and continuous through both the mass-spring-mass resonance and the coincidence region. The results from the analytical models show good agreement with measured results in both the single and double plate case. A simplified model is also presented for the sound reduction index of finite size floating floors. The model is valid for two elastic plates with a resilient layer in between where the bottom plate, the load-bearing slab, is assumed to be excited with a diffuse airborne sound field. The top plate and the resilient layer compose the floating floor. The problem is solved for frequencies below, between and above the critical frequencies of the plates. Above the critical frequency of the load-bearing plate, but below that of the floating slab, the main coupling between the plates will occur at the coincidence angle of the load-bearing plate. Above the critical frequency of both plates, the main transmission will occur at the angle of coincidence of each plate. As the plates will interact, the sound insulation improvement will to some extent depend on the properties of the load-bearing slab. It is shown how the sound reduction index depends on the physical parameters and the geometry of the plates. / QC 20101101

Thesis (M.S.)--State University of New York at Binghamton, Thomas J. Watson School of Engineering and Applied Science, Department of Electrical and Computer Engineering, 2007. / Includes bibliographical references.

När kraven på mätosäkerhet är hög vid geodetiska mätningar behövs geodetiska referenssystem realiserade av geodetiska nät av hög kvalitet. Etableringen och transformation till överordnade referenssystem i höjd av dessa nät genomförs idag ofta med terrestra metoder som avvägning, vilket är ett noggrant men tidskrävande arbete. Det finns flera försök att använda sig av andra metoder såsom GNSS, men en möjlighet skulle även vara att använda punktmoln från flygburen laserskanning. Detta arbetes syfte är att undersöka om punktmoln kan användas till transformation av ett geodetiskt höjdnät i Sandvikens kommun. Nätet består av cirka 500 fixpunkter och har 2010 transformerats till RH2000 av Lantmäteriet. Det använda punktmolnet har producerats av Lantmäteriet och har en medelavvikelse om 0,05 m på plana hårdgjorda ytor. Detta är relativt högt då vanligen osäkerheter på millimeternivå önskas vid transformationer. Men eftersom medeltal kan reducera slumpmässiga avvikelser i enskilda mätningar kan en transformation bestående av ett medelhöjdskift möjligen ge ett tillfredsställande resultat. Medelhöjdskiftet är då ett medeltal av flera höjdskift beräknade på olika ställen i punktmolnet. Genom att avväga höjdskillnaden mellan fixpunkterna i nätet och punkter på markytan som med olika metoder höjdbestäms utifrån punktmolnet har höjder för fixpunkterna enligt punktmolnet erhållits. Dessa har jämförts med RH2000 höjder enligt Lantmäteriets transformation för att beräkna en avvikelse, samt med de äldre lokala höjderna för att beräkna ett höjdskift. Genom att beräkna medelvärde och dess osäkerhet för höjdskiftet över hela nätet har en uppfattning om metodens lämplighet erhållits. Höjdskiften låg överlag inom några millimeter från det som Lantmäteriet beräknat, med 3 mm osäkerhet för den överlag bästa metoden. Även om höjdskiftets och därmed transformationens osäkerhet delvis blev något hög jämfört med avvägning så kan punktmoln ändå vara ett lämpligt alternativ. Speciellt i mera avlägsna områden utan bra anknytningar till det överordnade nätet kan det vara intressant. Det finns dock många parametrar som ännu inte utforskats, bland annat vissa eventuella systematiska avvikelser. / When the requirements on accuracy and precision are high for geodetic measurements you need geodetic reference systems realized with geodetic control networks of high quality. Today, establishment and transformation to higher order reference systems for height usually uses terrestrial methods like levelling. While highly accurate these result in time consuming work. There have been a couple attempts at using other methods for this task, for example GNSS, but another possibility might be usage of point clouds from airborne laser scanning. As a starting point for further studies this study attempts to use point clouds to transform a geodetic height network in Sandviken municipality, Sweden. The network consists of around 500 benchmarks and has been transformed to the national reference system for height, RH2000, by the Swedish national geodetic survey (Lantmäteriet) in 2010. The point cloud used is also produced by Lantmäteriet and is said to have a mean error of 0,05 m. This is relatively high since the requirements usually are in the millimeter range when determining transformation parameters, but if the transformation only consist of a single height shift calculated as a mean from several height shifts derived from the point cloud any random errors in the point cloud should be reduced. By measuring the height difference between benchmarks and points on the ground, that through different methods are given heights according to the point cloud, heights of the benchmarks have been determined according according to the point cloud. These can be compared to heights in RH2000 according to the transformation performed by Lantmäteriet to see their deviation from the assumed true value. Further comparisons against the older local heights of the benchmarks give a height shift that can be used as a simple transformation. By calculating a mean and uncertainty an estimation of the suitability of the method can be achieved. The all height shifts deviated a few millimeters from the result Lantmäteriet got, with uncertainties around 3 mm for the overall best method. Even if the uncertainty of the shift and therefore the transformation ended up somewhat high compared to what Lantmäteriet achieved it is still believed that point clouds may be or become a viable alternative. Especially in more remote regions without good connections to the higher order network. There are many parameters that have not yet been explored though, as well as some potential systematic errors that should be further investigated.

During processing of data received by an airborne radar one of the issues is that the typical signal echo from the ground produces a large perturbation. Due to this perturbation it can be difficult to detect targets with low velocity or a low signal-to-noise ratio. Therefore, a filtering process is needed to separate the large perturbation from the target signal. The traditional method include a tapered Fourier transform that operates in parallel with a MTI filter to suppress the main spectral peak in order to produce a smoother spectral output. The difference between a typical signal echo produced from an object in the environment and the signal echo from the ground can be of a magnitude corresponding to more than a 60 dB difference. This thesis presents research of how the multitaper approach can be utilized in concurrence with the minimum variance estimation technique, to produce a spectral estimation that strives for a more effective clutter suppression. A simulation model of the ground clutter was constructed and also a number of simulations for the multitaper, minimum variance estimation technique was made. Compared to the traditional method defined in this thesis, there was a slight improvement of the improvement factor when using the multitaper approach. An analysis of how variations of the multitaper parameters influence the results with respect to minimum detectable velocity and improvement factor have been carried out. The analysis showed that a large number of time samples, a large number of tapers and a narrow bandwidth provided the best result. The analysis is based on a full factorial simulation that provides insight of how to choose the DPSS parameters if the method is to be implemented in a real radar system.