Protein-Protein Interaktions (PPI) Netzwerke liefern ein Grundgerüst für systematische Untersuchungen der komplexen molekularen Maschinerie in der Zelle. Die Komplexität von Protein-Wechselwirkungen stellt jedoch in Bezug auf ihre Identifizierung, Validierung und Annotation eine große experimentelle und rechnerische Herausforderung dar. In dieser Arbeit analysierte ich diese Probleme und lieferte Lösungen, um die Limitierungen aktueller humanen PPI Netzwerke zu überwinden. Meine Arbeit kann in zwei Teile aufgeteilt werden: Im ersten Teil führte ich eine kritischen Vergleich von acht unabhängig konstruierten humanen PPI Netzwerke durch, um mögliche experimentellen Verzerrungen zu erkennen. Die Ergebnisse zeigten starke Tendenzen bezüglich der Selektion und Detektion von Interaktionen, die in zukünftigen Anwendungen dieser Netzwerke berücksichtigt werden sollten. Einer der wichtigsten Schlussfolgerungen dieser Studie war, dass die derzeitigen humanen Interaktions Netzwerke komplementär sind und deshalb wurde eine Datenbank mit der Bezeichnung Unified Human Interaktome (UniHI) entwickelt, die menschliche PPI Daten aus zwölf wichtigsten Quellen integriert. Im zweiten Teil dieser Forschungsarbeit benutzte ich die Daten aus der UniHI Datenbank, die genetischen Modifikatoren in einer bestimmten Krankheit, Chorea Huntington (HD) eine autosomal dominante neurodegenerative Erkrankung, zu charakterisieren. Um die Proteine zu identifizieren, die den Krankheitsverlauf modifizieren können, wurden Protein Interaktion Daten mit Genexpressionsdaten von HD-Patienten in Kombination mit einem Mehrschritt-Filterungsverfahren integriert. Mit dem neuartigen Ansatz wurde ein Nucleus caudatus-spezifische Protein-Interaktion HD (PPI)-Netzwerk vorhergesagt, das 14 potentiell dysregulierten Proteine direkt oder indirekt mit dem Huntingtin-Protein verlinkt, mit mögliche Verbindung zu Molekularen Prozessen wie z.B. Apoptose, Metabolismus, neuronale Entwicklung. / Protein interaction networks aim to provide the scaffold maps for systematic studies of the complex molecular machinery in the cell. The complexity of protein interactions poses, however, large experimental and computational challenges regarding their identification, validation and annotation. Additionally, storage and linking is demanding since new data are rapidly accumulating. In this research work, I addressed these issues and provided solutions to overcome the limitations of current human protein-protein interaction (PPI) maps. In particular, my thesis can be partitioned into two parts: In the first part, I conducted a comparative assessment of eight recently constructed human protein-protein interaction networks to identify experimental biases. Results showed strong selection and detection biases which are necessary to take into consideration in future applications of these maps. One of the important conclusions of this study was that the current human interaction networks contain complementary information; hence, a database was developed, termed as Unified Human Interactome (UniHI), integrating human PPI data from twelve major sources. Several new tools were included for querying, analyzing and visualizing human PPI networks. In the second part of this research work, UniHI dataset was applied to characterize the genetic modifiers involved in a specific disease: Chorea Huntington (HD), an autosomal dominant neurodegenerative disease. To find the modifiers, a network-based modeling approach was implemented by integrating huntingtin-specific protein interaction network with gene expression data from HD patients in multiple steps. Using this approach, a Caudate Nucleus-specific HD protein interaction (PPI) network was predicted, connecting 14 potentially dysregulated proteins directly or indirectly to the disease protein, showing a possible link to molecular processes such as pro-apoptotic pathways, cell survival, anti-apoptotic, growth, and neuronal diseases.

Made available in DSpace on 2016-04-28T14:58:35Z (GMT). No. of bitstreams: 1 2006-Joao Batista Alves Pereira.pdf: 1832144 bytes, checksum: 36798c7d302cc3a5d500e230c9f5af03 (MD5) Previous issue date: 2006-02-21 / Evaluation of the vegetal growth, hydric necessity and water use efficiency by bell pepper crop (Capsicum annum. L.) under organic management in system of seeding with prepare soil and no till cropping system. This work was conducted in the experimental area of SIPA (Integrated system of agro ecological production), at the county of Serop?dica RJ, with the aim of valuate the growth , the water needs, and the efficiency of the use of the water by the bell pepper culture (Capsicum annuum. L ), at the conventional (PC) and the direct systems of soiling. The conventional system consisted of soil prepare with rotational hoe tied to a micro tractor, and the direct soiling, being done after the occupation of the area with a combination of Sorghum bicolor and Crotal?ria acroleura, that was grazed at the transplantation of the bell pepper s seedlings without previous soil preparation. The same fertilization and cultural practices permitted by the agro ecological handling, was used in both systems. The two systems tested, received sprinkler irrigation, and the determination of the water s blade, was made by monitoring with TDR (Time Domain Reflectometry), calibrated for the soil conditions of the experimental area. The splits were also monitorated by a tensiometer at a 50 cm. deep. Collects were made at intervals of 14 days, with the aim of foliar area evaluation, biomass accumulation by the parts of the plant, along the entire cycle, being also evaluated the crop production at the two systems of soiling. By the adjustment of the IAF (Foliar Area Indices ) and the biomass accumulated by the plant at the two systems of planting in function of accumulated day-degree (GDA), was verified that 1533 GDA was necessary to complete the productive cycle, reaching the top of accumulated biomass at 1059 and 1148 GDA, at PC and PD respectivement. There were no differences between the systems of planting, in respect of the growth rate and the productivity, however the PD system gave less competition between spontaneous vegetation and the bell pepper crop, reducing in 60% the necessity of hand labor at the firsts weddings. The PD system raise the efficiency of the use of water (EUA) by the crop reducing in 47,5 mm. the blade applied during its cycle, in relation of PC. The irrigation handling using TDR, provide an meaning EUA of 4,4 Kg.m-3, above the means of 1,5 to 3,0Kg.m-3, recommended by Doorenbos & Kassam (1994). The culture coefficients (Kc) recommended for the bell pepper crop, were evaluated together with the calculated coefficients using some methodologies proposed in this work, and was noted that the estimated Kc s values of the production cycles (ETc cycle) and by the Kc curve (graphic ETc) estimated during the culture cycle, were more appropriated for the calculation of the evapotranspiration of the crop. The estimation of reference evapotranspiration (ETo), by the pan class A method, made possible means close to those estimated by the Penman-Monteith model witch is recommended by FAO as standard. Therefore, the pan class A method, can be utilized in the estimated ETo, at same conditions of the experiment when there are no data of climate for the Penman-Monteith model use. Associated tensiometry and TDR for irrigation management, showed to be an essential practice when looking for raising the efficiency of the use of water. / Este trabalho foi realizado na ?rea experimental do SIPA (Sistema Integrado de produ??o Agroecol?gica), localizada no munic?pio de Serop?dica-RJ, com o objetivo de avaliar o crescimento, as necessidades h?dricas e a efici?ncia no uso da ?gua pela cultura do piment?o (Capsicum annuum. L), nos sistemas de plantio convencional (PC) e direto (PD). O sistema convencional consistiu de preparo de solo com enxada rotativa acoplada a micro-trator e o plantio direto, na ocupa??o da ?rea com pr?-cultivo, utilizando o cons?rcio Sorghum bicolor e Crotal?ria acroleura, os quais foram ro?ados por ocasi?o do transplante das mudas de piment?o, sem o preparo pr?vio do solo. Em ambos os tratamentos a cultura recebeu a mesma aduba??o de cova e tratos culturais permitidos pelo manejo agroecol?gico. Nos dois sistemas de plantio a cultura foi irrigada por aspers?o, e a determina??o das l?minas aplicadas, foi realizada atrav?s do monitoramento com TDR (Reflectometria no dom?nio do tempo), calibrado para as condi??es de solo da ?rea experimental. As parcelas foram monitoradas tamb?m por tensi?metro no perfil de 50cm de profundidade. Foram realizadas coletas em intervalos de 14 dias com a finalidade de avaliar a ?rea foliar e acumulo de biomassa pelas partes da planta, durante todo o seu ciclo, sendo avaliado tamb?m a produ??o pela cultura nos dois sistemas de plantio. Por meio do ajuste do IAF e da biomassa acumulada pela planta nos dois sistemas de plantio em fun??o de graus-dia acumulado (GDA), constatou-se que s?o necess?rios 1533 GDA para que a planta complete o seu ciclo produtivo, atingindo o m?ximo de biomassa acumulada aos 1059 e 1148 GDA, no PC e PD, respectivamente. Os sistemas de plantio n?o diferiram significativamente entre si com rela??o ?s taxas de crescimento e produtividade, entretanto o plantio direto proporcionou menor competi??o da vegeta??o espont?nea com a cultura, reduzindo em 60 % a necessidade de m?o-de-obra nas primeiras capinas. O PD aumentou a efici?ncia no uso da ?gua (EUA), pela cultura, reduzindo em 47,5mm a l?mina aplicada durante o seu ciclo, em rela??o ao PC. O manejo da irriga??o utilizando o TDR, proporcionou a EUA m?dia de 4,4kg.m-3, acima dos valores 1,5 a 3,0kg.m-3 propostos por Doorenbos e Kassam (1994). Os Coeficientes de cultivo (Kc) recomendados para a cultura, foram avaliados juntamente com os calculados por v?rias metodologias propostas neste trabalho, verificando-se que os valores de Kc estimados em fun??o dos ciclos de produ??o (ETc ciclo) e pela curva de Kc (ETc gr?fico) estimados durante o ciclo da cultura, se mostraram mais adequados ao c?lculo da evapotranspira??o da cultura. A estimativa da evapotranspira??o refer?ncia (ETo), pelo m?todo do tanque classe A , possibilitou valores pr?ximos aos estimados pelo modelo de Penman-Monteith recomendado pela FAO como padr?o. Portanto o m?todo do tanque classe A poder? ser utilizado na estimativa da ETo, em condi??es semelhantes a do experimento, quando n?o houver disponibilidade de dados clim?ticos para o uso do modelo de Penman- Monteith. O uso da tensiometria associado ao TDR no manejo de irriga??o revelou ser uma pr?tica indispens?vel, quando se almeja aumentar a efici?ncia no uso da ?gua.

Made available in DSpace on 2016-03-15T19:47:36Z (GMT). No. of bitstreams: 1 Fred Izumi Utsunomiya.pdf: 7103886 bytes, checksum: 47b2bc05bda7594a4675aaa16accff9e (MD5) Previous issue date: 2014-08-12 / Fundo Mackenzie de Pesquisa / Kenjinkai is an association established in Brazil which gathers immigrants from an specific Japanese prefecture (province) and its descendents. Its objective is to work as a liaison between its members and their original province. Japan has 47 provinces and there are 47 associations of this kind in Brazil, which are formed by a Nikkei Brazilian citizen public. Those Japanese rooted associations are strongly connected to the provinces of their founders. Considering that its been fifty years since the closing of the Japanese immigration period in Brazil happened, period when it was developed a process of adaptation and inclusion of these immigrants in the Brazilian society, how do they present themselves? As Brazilian entities or Japanese ones? This thesis investigates the cultural identity which is built by these Kenjinkai in Brazil through the discourses of their institutional websites. The theory and methodology of narrative and discoursive semiotics were chosen to analyze three kenjinkai's websites. From the perspective proposed by the Cultural Studies about the construction of cultural identity trough the discourse, we concluded that the actor of enunciation of Kenjinkai in Brazil is A Brazilian actor and has a cultural Brazilian identity. / O kenjinkai é uma associação estabelecida no Brasil que reúne imigrantes de uma determinada província japonesa e seus descendentes, com o objetivo de servir como elo de ligação entre os associados e a província de origem. O Japão possui 47 províncias e no Brasil existem 47 kenjinkai, que são formados predominantemente por um público Nikkei de nacionalidade brasileira. Essas organizações de origem japonesa estão profundamente ligadas às províncias de seus fundadores, mas passados quase cinquenta anos após o encerramento do ciclo de imigração, quando desenvolveu-se o processo de adaptação e absorção desses imigrantes na sociedade brasileira, o que elas professam ser? Entidades japonesas ou brasileiras? Esta tese investiga qual é a identidade cultural construída pelos kenjinkai no Brasil através dos discursos dos sites dessas instituições. Três sites foram selecionados para análise segundo metodologia proposta pela semiótica narrativa e discursiva e, a partir da perspectiva proposta pelos Estudos Culturais de construção de identidade cultural a partir do discurso, levantou-se que o ator da enunciação dos kenjinkai no Brasil é um ator brasileiro e que possui uma identidade cultural brasileira.

This thesis deals with creating hardware and software equipment of intelligent beehive for data collection purpose. Measurement characteristics are temperature, humidity, weight of beehive and audio record of bees. They are measured by available IoT technology. They are simple single-chip component with a clear focus. This thesis also include creation of software for measurements this characteristics and communications with server. The software featured of the remote server are also documented. This software analyze incoming measurement and also provides their displays. Software in this thesis is developed in language Python.

本研究主要在建立1996-2008年間建商的存活預警模型，探討眾多危機事件中對於建商存活的影響力分析。研究所稱建商包括「建設公司」以及有從事建設業的相關公司。在台灣地區位屬火車頭角色的建設業具有舉足輕重的影響力，但其營運的財務槓桿較其他產業高，營運狀況易受景氣波動的影響，因此在事件的產生時對於投資者、銀行的應變處理便顯得相對重要。 危機事件所指除了證管會、台灣經濟新報資料庫的危機認定外，另加入財務之外的事件研究並加以定義。存活預警模型的建立是先以各種事件的相關性分析以了解各種事件的顯著性，並且量化分類後的影響事件，再進行羅吉特分析與存活分析的比對，選擇較適模型與影響力較大的危機事件進行實證分析。研究實證結果如下： 一 危機事件越多，建商經營越不穩定，存亡事件產生機率越高；影響公司存活的共通因子對於公司危機產生的共同預警變數有一定程度上的共通性，且都具有顯著性。 二 本研究將危機事件分類為財務面、監理面、經營面，存亡事件選定有終止上市、全額交割股、掏空三種。經實證後得知，財務面之外的預警變數亦能提供投資人及銀行產業了解公司經營的穩定度。在眾多預警變數中，又以重整、紓困跳票違約、董監事改選對於終止上市影響較大；對全額交割股的影響則以董監事改選、警示股、景氣因素以及利益輸送影響較大；對掏空的影響則以重整、警示股、財務結構較具顯著性。 三 存活期間與存活機率相關性比較中，以景氣預警變數而言，若產生全額交割事件，則產生存亡事件的機率非常高，公司得以存活期間較短。以重整預警變數而言，公司重整後，產生存亡事件的機率非常高，也就是說公司得以存活的期間較其他預警變數短。 四 景氣循環對於建商經營有非常大的影響，不景氣時產生具顯著性的危機事件，將會在較短期間後產生存亡事件。 / The goal of our study is establishing the survival model in the building construction companies during 1996-2008. The real estate developers in this study included “building construction companies” and the “property stock companies” which operate building construction businesses. Building construction companies are common known as a pivotal role in the power of influence by the general public, but the financial leverage is higher than other industries. Operating condition of construction companies is effected by the real estate cycle; in hence it is relative importance of the investers, banks, which the crisis events occurred. The crisis events in this study are not only the definition of Taiwan Stock Exchange and Taiwan Economic Journal Data Bank but also the events besides financial side. The study of survival model, in the beginning, is to analyze the correlation of crisis events to understand the significance of events, quantify the classified of the entire crisis event, and then to compare the results between the Logistic Analysis and the Survival Analysis. Finally, choose the appropriate model and the more influence crisis events to do the empirical analysis. The empirical analysis results of our study are as follows: 1. The more the crisis events occurred, the more the probability of failure events will occur. 2. Our study classified the crisis events into three different species, financial side, supervision side, business side. We choose company delisting, full delivery stock company, and company emptied as the failure events. 3. Compare the correlation of survival period with survival probability. In terms of company reorganized variable, for example, after reorganizing the building construction company, the probability of failure events are getting higher, and the survival duration get shorter than other crisis events. 4. The real estate cycle impact the building construction companies a lot. If significant crisis events occurred during downturn of the real estate cycle, the failure events will be generated in such a short time.

<p>Abstract</p><p>The aim of this study is to analyze how the Swedish company VisitSweden and The Swedish institute creates an image of Sweden and what that image looks like? And also to compare their goals and their ways of present Sweden to find out how that contributes to the images of Sweden. The method for this analyze is chosen from the work and theories of Norman Fairclough called “critical discourse analyze.” The analysis focuses on the text and how the text is created and if the author’s opinions are hidden in the words. The method can be applied in many ways. I choose to focus on is what kind of themes the author presents in the text and what meaning the author gives the themes. The other one I focused on is how the author addresses the reader of the text trough the choice of words and sentences. The third one is analyzing how the author says thing in the text, if the author agrees with certain statements ore if the author takes a very authoritarian expression in the way of telling things to the readers, which is taken from the work of Roger Fowlers. The material used in the analyzing parts is taken from books concerning discourse analyze. More material is taken from interviewing several employees in VisitSweden and the Swedish institute. The main result of the essay is that the image of Sweden is depending on which company who presents it and their individual goals. The main thing is that Sweden is more and more becoming a brand and that the companies want to sell that brand in different ways.</p><p>Number of pages: 45</p><p>Course: Media and Communication Studies C</p><p>Period: Fall 2006/2007</p><p>University: Division of Media and Communication, Department of Information Science, Uppsala University</p><p>___________________________________________</p><p>Key words:</p><p>The image of Sweden, the Swedish institute, VisitSweden, critical discourse analyze, Sweden as a brand, selling a country, goals to make PR for a country, how to present a country to foreigners.</p>

Abstract The aim of this study is to analyze how the Swedish company VisitSweden and The Swedish institute creates an image of Sweden and what that image looks like? And also to compare their goals and their ways of present Sweden to find out how that contributes to the images of Sweden. The method for this analyze is chosen from the work and theories of Norman Fairclough called “critical discourse analyze.” The analysis focuses on the text and how the text is created and if the author’s opinions are hidden in the words. The method can be applied in many ways. I choose to focus on is what kind of themes the author presents in the text and what meaning the author gives the themes. The other one I focused on is how the author addresses the reader of the text trough the choice of words and sentences. The third one is analyzing how the author says thing in the text, if the author agrees with certain statements ore if the author takes a very authoritarian expression in the way of telling things to the readers, which is taken from the work of Roger Fowlers. The material used in the analyzing parts is taken from books concerning discourse analyze. More material is taken from interviewing several employees in VisitSweden and the Swedish institute. The main result of the essay is that the image of Sweden is depending on which company who presents it and their individual goals. The main thing is that Sweden is more and more becoming a brand and that the companies want to sell that brand in different ways. Number of pages: 45 Course: Media and Communication Studies C Period: Fall 2006/2007 University: Division of Media and Communication, Department of Information Science, Uppsala University ___________________________________________ Key words: The image of Sweden, the Swedish institute, VisitSweden, critical discourse analyze, Sweden as a brand, selling a country, goals to make PR for a country, how to present a country to foreigners.

Ce manuscrit est consacré à l'étude des transformations polymorphiques induites au cours du procédé de compression directe, et à son incidence sur les propriétés mécaniques des comprimés. L'objectif principal de ce travail est d'apporter des éléments de compréhension sur la transition polymorphique de la caféine (principe actif modèle) Forme I en Forme II survenant lors du procédé de compression directe, et de déterminer si celle-ci a un impact sur la contrainte à la rupture du comprimé. L'utilisation du simulateur de compression Styl'One Classique (Médel'Pharm) et d'une machine de fatigue (Instron®) pour la fabrication des comprimés, a permis d'étudier deux paramètres de procédé (pression et vitesse de fabrication) et deux paramètres de formulation (dilution du principe actif et nature du diluant) représentatifs de conditions industrielles. Les transitions de phase de la caféine ont été évaluées par analyse calorimétrique différentielle (ACD). De plus, des études cinétiques ont été conduites durant plusieurs mois afin d'observer l'influence de ces différents paramètres sur la transition polymorphique de la caféine anhydre Forme I en Forme II dans les comprimés au cours de leur stockage. Enfin, l'analyse du mécanisme de transition de ce principe actif a été réalisée au moyen d'une loi exponentielle étirée, issue du modèle de Johnson-Mehl-AvramiLa contrainte à la rupture des comprimés (caractéristique globale) a été mesurée par un test de rupture diamétrale, la dureté de surface des comprimés (caractéristique locale) par nano-indentation. Un premier modèle de prédiction de la contrainte à la rupture selon la teneur en caféine a été développé. Les principales caractéristiques du cycle de compression calculées à partir des données enregistrée par le simulateur de compression ont permis d'analyser le comportement des formules lors de la compression puis d'établir un second modèle de prédiction de la contrainte à la rupture.Les résultats de transition polymorphique et de propriétés physiques de comprimés seront alors confrontés / Direct compression process is widely used in the pharmaceutical industry for tablet manufacturing. This work is dedicated to the study of the polymorphic transformation induced by a direct compression process, and its impact on tablet mechanical properties. The main objective is to improve the understanding of the phase transition of caffeine Form I into Form II occurring during the direct compression process, and whether it has an impact on the tablet tensile strength. In this way, several studies have been conducted on the impact of operating conditions on the polymorphic transformation of a model active pharmaceutical ingredient (API) and on few physical properties of the tablets.The use of a compression simulator Styl’One Classique (Médel’Pharm) and a fatigue equipment (Instron®) for the manufacture of tablets, allowed studying two process parameters (compression load and compression speed) and two formulation parameters (dilution of the API and nature of the diluent). Caffeine phase transitions have been evaluated by differential scanning calorimetry (DSC). Moreover, during several months after tableting, kinetic studies were conducted in order to observe the influence of these parameters on the polymorphic transition of the anhydrous caffeine Form I into Form II in tablets during storage. Finally, the analysis of the transition mechanism of this API was performed thanks to a stretched exponential law, derived from the Johnson-Melh-Avrami model.The tensile strength of tablets (global property) was measured by a diametral compression test and their surface hardness (local property) by nanoindentation. A first predictive model for tablet tensile strength according to the caffeine content was developed. The compression cycle characteristics calculated from the data recording with the compression simulator allowed analyzing the behavior of different blends during the compression process. A second model for predicting the tensile strength was then established.Finally, results obtained for the polymorphic transition and physical properties of tablets will then be confronting

In this dissertation we seized less conspicuous attractions which are situated in the choice locations of Tábor landscape. We endeavoured to discover other possibilities of utilization of the chosen destination, despite our initial impression that the destination was fully used. The most important task of this thesis was to analyze natural, historical and natural interests of this region. After detailed research/analyzes/discussions we discovered that there are a lot of ``hidden{\crq}q attractions in the locality. These attractions deserve their renewal and more attention from their visitors. On the base of information were created suggestions and recommendations for development of this destination.

The main objective of the diploma work was to analyze possibilities of export financing of chosen enterprise subject and to suggest optimal solution. Partial target was to draw up strategy of export support forms, export services and supportive programs for selected enterprise. Subject of this work is company FARMTEC, a. s., active in production of agricultural technology and its sale on Czech and foreign markets. Investment loan, overdraft financing and supplier credit are the prevailing sources of financing used by this company. As well cooperation with companies EGAP, ČEB and CzechTrade is maintained. Financing product TOP Export II offered by Česká spořitelna is found as the best solution for company FARMTEC, a. s. It comprises factoring financing. Further it is recommended to use a new against-crisis product currently offered by EGAP. It is proposed to present company in official address book of Czech export companies offered by CzechTrade.