Le 1,4,7-triazacyclononane (tacn) fait partie de la famille des polyazamacrocycles et a la faculté, lorsqu’il est N-fonctionnalisé par des groupements coordinants adéquats, de former des complexes de coordination stables thermodynamiquement et inertes cinétiquement avec les métaux de transition. C’est pourquoi les dérivés du tacn trouvent de nombreuses applications dans de nombreux domaines, par exemple celui de l’imagerie médicale. Notre équipe a développé des ligands N-fonctionnalisés par des groupements picolinate et méthylpyridine pour des applications en imagerie TEP et IRM.Le premier travail de cette thèse porte sur l’élaboration de nouvelles sondes bimodales TEP-imagerie optique et IRM-imagerie optique à motifs issus de tacn-picolinate décrits au laboratoire.Une seconde partie est axée sur la synthèse de nouveaux chélates du Cu(II) à base de tacn portant des bras méthylthiazole pour une potentielle application en imagerie TEP. Les complexes de Cu(II) correspondant ont été étudiés par différentes méthodes analytiques et spectroscopiques afin de juger leurs stabilités thermodynamiques et leurs inerties cinétiques.Les chélatants répondant aux critères imposés par le milieu biologique ont été sélectionnés pour une application en imagerie TEP dans le cadre du cancer de la prostate. Des analogues bifonctionnels ont ainsi été élaborés afin de les incorporer dans des radiopharmaceutiques radiomarqués au cuivre-64. / The 1,4,7-triazacyclononane (tacn) is part of the family of polyazamacrocycles, and has the faculty, when appropriately N-functionalized with coordination pendants, to form thermodynamically stable and kinetically inert coordination complexes with transition metals. Thanks to their properties tacn derivatives can be found in numerous applications such as medical imaging. Our group has developed some ligands N-functionalized with picolinate and picolyl pendants for an application in PET imaging and MRI.The first part of this thesis is focused on the development of a new class of bimodal PET-optical imaging and MRI-optical imaging probes based on tacn-picolinate units described in the laboratory.The second part deals with the synthesis of new Cu(II) chelators based on tacn-methylthiazolyl units for a potential application in PET imaging. The corresponding Cu(II) complexes were studied by different analytical and spectroscopic methods in order to evaluate their thermodynamic stabilities and kinetic inertness.The chelators that fullfiled all the criterias imposed by the biological media were selected for an applicationPET imaging of prostate cancer. Some bifunctional analogues were elaborated so as to incorporate them in radiopharmaceuticals radiolabeled with copper-64.

Peu d'études ont été menées sur l'influence de la stéréoscopie sur la perception d'un mixage audio au cinéma. Les témoignages de mixeurs ou les articles scientifiques montrent pourtant une grande diversité d'opinions à ce sujet. Certains estiment que cette influence est négligeable, d'autres affirment qu'il faut totalement revoir notre conception de la bande-son, aussi bien au niveau du mixage que de la diffusion. Une première série d'expériences s'est intéressée à la perception des sons d'ambiance. 8 séquences, dans leurs versions stéréoscopiques (3D-s) et non-stéréoscopiques (2D), ont été diffusées dans un cinéma à des sujets avec plusieurs mixages différents. Pour chaque présentation, les sujets devaient évaluer à quel point le mixage proposé leur paraissait trop frontal ou au contraire trop « surround », le but étant de mettre en évidence une éventuelle influence de la stéréoscopie sur la perception de la balance frontal/surround d'un mixage audio. Les résultats obtenus ont rejoint ceux d'une expérience préliminaire menée dans un auditorium de mixage, où les sujets se trouvaient en situation de mixeur et devaient eux-mêmes régler la balance frontal/surround : l'influence de la stéréoscopie était faible et n'apparaissait que pour quelques séquences. Des études ont ensuite été menées sur la perception des objets sonores tels que dialogues et effets. Une quatrième expérience s'est intéressée à l'effet ventriloque en élévation : lorsque l'on présente à un sujet des stimuli audio et visuel temporellement coïncidents mais spatialement disparates, les sujets perçoivent parfois le stimulus sonore au même endroit que le stimulus visuel. On appelle ce phénomène l’effet ventriloque car il rappelle l'illusion créée par le ventriloque lorsque sa voix semble plutôt provenir de sa marionnette que de sa propre bouche. Ce phénomène a été très largement étudié dans le plan horizontal, et dans une moindre mesure en distance. Par contre, très peu d'études se sont intéressées à l'élévation. Dans cette expérience, nous avons présenté à des sujets des séquences audiovisuelles montrant un homme en train de parler. Sa voix pouvait être reproduite sur différents haut-parleurs, qui créaient des disparités plus ou moins grandes en azimut et en élévation entre le son et l'image. Pour chaque présentation, les sujets devaient indiquer si la voix semblait ou non provenir de la même direction que la bouche de l'acteur. Les résultats ont montré que l'effet ventriloque était très efficace en élévation, ce qui suggère qu'il n'est peut-être pas nécessaire de rechercher la cohérence audiovisuelle en élévation au cinéma. / Few psychoacoustic studies have been carried out about the influence of stereoscopy on the sound mixing of movies. Yet very different opinions can be found in the cinema industry and in scientific papers. Some argue that sound needs to be mixed differently for stereoscopic movies while others pretend that this influence is negligible.A first set of experiments was conducted, which focused on the perception of ambience. Eight sequences - in their stereoscopic (s-3D) and non-stereoscopic (2D) versions, with several different sound mixes - were presented to subjects. For each presentation, subjects had to judge to what extent the mix sounded frontal or “surround.” The goal was to verify whether stereoscopy had an influence on the perception of the front/surround balance of ambience. Results showed that this influence was weak, which was consistent with a preliminary experiment conducted in a mixing auditorium where subjects had to mix the front/surround balance of several sequences themselves.Studies were then conducted on the perception of sound objects such as dialogs or on-screen effects. A fourth experiment focused on ventriloquism in elevation: when presented with a spatially discordant auditory-visual stimulus, subjects sometimes perceive the sound and the visual stimuli as coming from the same location. Such a phenomenon is often referred to as ventriloquism, because it evokes the illusion created by a ventriloquist when his voice seems to emanate from his puppet rather than from his mouth. While this effect has been extensively examined in the horizontal plane and to a lesser extent in distance, few psychoacoustic studies have focused on elevation. In this experiment, sequences of a man talking were presented to subjects. His voice could be reproduced on different loudspeakers, which created disparities in both azimuth and elevation between the sound and the visual stimuli. For each presentation, subjects had to indicate whether or not the voice seemed to emanate from the mouth of the actor. Ventriloquism was found to be highly effective in elevation, which suggests that audiovisual coherence in elevation might be unnecessary in theaters.

Motion events are ubiquitous in conversation, from describing a tiresome commute to recounting a burglary. These situations, where an entity changes location, consist of four main semantic components: Motion (the movement), Figure (the entity moving), Ground (the object or objects with respect to which the Figure carries out the Motion) and Path (the route taken). Two additional semantic components can occur simultaneously: Manner (the way the Motion occurs) and Cause (the source of/reason for the Motion). Languages differ in preferences for provision and packaging of semantic components in descriptions. It has been suggested, in the thinking-for-speaking hypothesis, that these preferences influence the conceptualisation of events (such as their memorisation). This thesis addresses questions relating to the description and memory of Motion events in British Sign Language (BSL) and English. It compares early BSL (acquired before age seven) and late BSL (acquired after age 16) descriptions of Motion events and investigates whether linguistic preferences influence memory. Comparing descriptions by early signers and late signers indicates where their linguistic preferences differ, providing valuable knowledge for interpreters wishing to match early signers. Understanding how linguistic preferences might influence memory contributes to debates around the connection between language and thought. The experimental groups for this study were: deaf early BSL signers, hearing early BSL signers, deaf late BSL signers, hearing late BSL signers and hearing English monolinguals. Participants watched target Motion event video clips before completing a memory and attention task battery. Subsequently, they performed a forced-choice recognition task where they saw each target Motion event clip again alongside a distractor clip that differed in one semantic component. They selected which of the two clips they had seen in the first presentation. Finally, participants were filmed describing all of the target and distractor video clips (in English for English monolinguals and BSL for all other groups). The Motion event descriptions were coded for the inclusion and packaging of components. Linguistic descriptions were compared between languages (English and BSL) and BSL group. Statistical models were created to investigate variation on the memory and attention task battery and the recognition task. Results from linguistic analysis reveal that English and BSL are similar in the components included in descriptions. However, packaging differs between languages. English descriptions show preferences for Manner verbs and spatial particles to express Path ('run out'). BSL descriptions show preferences for serial verb constructions (using Manner and Path verbs in the same clause). The BSL groups are also similar in the components they include in descriptions. However, the packaging differs, with hearing late signers showing some English-like preferences and deaf early signers showing stronger serial verb preferences. Results from the behavioural experiments show no overall relationship between language group and memory. I suggest that the similarity of information provided in English and BSL descriptions undermines the ability of the task to reveal memory differences. However, results suggest a link between individual linguistic description and memory; marking a difference between components in linguistic description is correlated with correctly selecting that component clip in the recognition task. I argue that this indicates a relationship between linguistic encoding and memory within each individual, where their personal preference for including certain semantic components in their utterances is connected to their memory for those components. I also propose that if the languages were more distinct in their inclusion of information then there may have been differences in recognition task scores. I note that further research is needed across modalities to create a fuller picture of how information is included and packaged cross-modally and how this might affect individual Motion event memory.

La présente dissertation est principalement consacrée à la conception et à la caractérisation d’une sonde universelle pour l’imagerie volumétrique ultrasons-optoacoustique et le développement d’un algorithme de reconstruction adapté aux exigences physiques pour la conception du système. Les traits distinctifs de cette dissertation sont l’introduction d’une nouvelle géométrie pour les sondes manuelles ultrasons-optoacoustique et des évaluations systématiques basées sur des méthodes de pré-reconstruction et post-reconstruction. Pour éviter l’interprétation biaisée, une évaluation capable d’évaluer le potentiel de la sonde doit être faite. Les caractéristiques mentionnées établissent un cadre pour l’évaluation des performances du système d’imagerie d’une manière précise. En outre, elle permet d’optimiser les performances suivant l’objectif fixé. Ainsi, deux algorithmes de reconstruction anticipée ont été élaborés pour la conception du système OPUS (optoacoustique ultrasons) capables de produire des images avec un contraste et une résolution homogènes sur tout le volume d’intérêt. L’intérêt d’avoir de tels algorithmes est principalement dû au fait que l’analyse des données médicales est souvent faite dans des conditions difficiles, car on est face au bruit, au faible contraste, aux projections limités et à des transformations indésirables opérées par les systèmes d’acquisition. Cette thèse montre, aussi, comment les artefacts de reconstruction peuvent être réduits en compensant les propriétés d’ouverture et en atténuant les artefacts dus à l’échantillonnage angulaire parcimonieux. Afin de transférer cette méthodologie à la clinique et de valider les résultats théoriques, une plate-forme d’imagerie expérimentale a été développée. En utilisant le système de mesure développé, l’évolution d’une nouvelle géométrie annulaire parcimonieuse et sa dynamique ont été étudiées et une preuve de concept a été démontrée à travers des mesures expérimentales dans le but d’évaluer les progrès réalisés. / When the interest is in multiscale and multipurpose imaging, there exists such a will in integrating multi-modalilties into a synergistic paradigm in order to leverage the diagnostic values of the interrogating agents. Employing multiple wavelengths radiation, optoacoustic imaging benefits from the optical contrast to specifically resolve molecular structure of tissue in a non-invasive manner. Hybridizing optoacoustic and ultrasound imaging comes with the promises of delivering the complementary morphological, functional and metabolic information of the tissue. This dissertation is mainly devoted to the design and characterization of a hybridized universal handheld probe for optoacoustic ultrasound volumetric imaging and developing adaptive reconstruction algorithms toward the physical requirements of the designed system. The distinguishing features of this dissertation are the introduction of a new geometry for optoacoustic ultrasonic handheld probe and systematic assessments based on pre and post reconstruction methods. To avoid the biased interpretation, a de facto performance assessment being capable of evaluating the potentials of the designed probe in an unbiased manner must be practiced. The aforementioned features establish a framework for characterization of the imaging system performance in an accurate manner. Moreover, it allows further task performance optimization as well. Correspondingly, two advanced reconstruction algorithms have been elaborated towards the requirement of the designed optoacoustic-ultrasound (OPUS) imaging system in order to maximize its ability to produce images with homogeneous contrast and resolution over the entire volume of interest. This interest is mainly due to the fact that the medical data analysis pipeline is often carried out in challenging conditions, since one has to deal with noise, low contrast, limited projections and undesirable transformations operated by the acquisition system. The presented thesis shows how reconstruction artifacts can be reduced by compensating for the detecting aperture properties and alleviate artifacts due to sparse angular sampling. In pursuit of transferring this methodology to clinic and validating the theoretical results, a synthetic imaging platform was developed. Using the measurement system, the evolution of a novel sparse annular geometry and its dynamics has been investigated and a proof of concept was demonstrated via experimental measurement with the intention of benchmarking progress.

La récente découverte des carbon dots (CDs) et de leurs propriétés physico-chimiques exceptionnelles (stabilité chimique, solubilité en milieu aqueux, faible toxicité, biocompatibilité, photoluminescescence et résistance au photoblanchiment) permet d’envisager l’utilisation de ces matériaux carbonés de taille nanométrique dans de nouvelles approches en imagerie biomédicale (fluorescence, IRM...), pour la vectorisation d’acides nucléiques (ADN, siARN) et la délivrance d’actifs thérapeutiques. Dans ce contexte, les objectifs de ce travail de thèse s’articulent autour de deux thématiques bien précises : échantillonnage de nanoparticules carbonées et développement de plateformes théranostiques. Une première partie a ainsi été consacrée à la préparation de carbon dots diversement fonctionnalisés de façon à pouvoir explorer l’espace structural et mener des études de relation structure-toxicité sur différentes lignées de cellules en culture. La seconde partie a été centrée sur l’élaboration d’une plateforme théranostique à base de carbon dots visant, d’une part, à délivrer un acide nucléique de façon intracellulaire et, d’autre part, à permettre un suivi des particules par différentes techniques d’imagerie. / The recent discovery of carbon dots (CDs) and their very interesting phsico-chemical properties (chemical stability, water solubility, low toxicity, biocompatibility, photoluminescence and resistance to photobleaching) make these carbon nanoparticles a powerfull platform for biomedical imaging (fluorescence, MRI...), nucleic acids vectorization (DNA, siRNA) and drug delivery. In this context, the objectives of the thesis work are divided into two different thematics: carbon nanoparticles sampling and development of theranostic platforms. The first part is devoted to the preparation of various functionalized carbon dots to explore the structural space and to manage structure-toxicity relationship studies on different cell lines. The second part is focused on the development of a theranostic platform based on carbon dots in order to promote simultaneously nucleic acids delivery into cells and to monitor them by different imaging techniques.

Odour localisation is the problem of finding the source of an odour or other volatile chemical. It promises many valuable practical and humanitarian applications. Most localisation methods require a robot to reactively track an odour plume along its entire length. This approach is time consuming and may be not be possible in a cluttered indoor environment, where airflow tends to form sectors of circulating airflow. Such environments may be encountered in crawl-ways under floors, roof cavities, mines, caves, tree-canopies, air-ducts, sewers or tunnel systems. Operation in these places is important for such applications as search and rescue and locating the sources of toxic chemicals in an industrial setting. This thesis addresses odour localisation in this class of environments. The solution consists of a sense-map-plan-act style control scheme (and low level behaviour based controller) with two main stages. Firstly, the airflow in the environment is modelled using naive physics rules which are encapsulated into an algorithm named a Naive Reasoning Machine. It was used in preference to conventional methods as it is fast, does not require boundary conditions, and most importantly, provides approximate solutions to the degree of accuracy required for the task, with analogical data structures that are readily useful to a reasoning algorithm. Secondly, a reasoning algorithm navigates the robot to specific target locations that are determined with a physical map, the airflow map, and knowledge of odour dispersal. Sensor measurements at the target positions provide information regarding the likelihood that odour was emitted from potential odour source locations. The target positions and their traversal are determined so that all the potential odour source sites are accounted for. The core method provides values corresponding to the confidence that the odour source is located in a given region. A second search stage exploiting vision is then used to locate the specific location of the odour source within the predicted region. This comprises the second part of a bi-modal, two-stage search, with each stage exploiting complementary sensing modalities. Single hypothesis airflow modelling faces limitations due to the fact that large differences between airflow topologies are predicted for only small variations in a physical map. This is due to uncertainties in the map and approximations in the modelling process. Furthermore, there are uncertainties regarding the flow direction through inlet/outlet ducts. A method is presented for dealing with these uncertainties, by generating multiple airflow hypotheses. As the robot performs odour localisation, airflow in the environment is measured and used to adjust the confidences of the hypotheses using Bayesian inference. The best hypothesis is then selected, which allows the completion of the localisation task. This method improves the robustness of odour localisation in the presence of uncertainties, making it possible where the single hypothesis method would fail. It also demonstrates the potential for integrating naive physics into a statistical framework. Extensive experimental results are presented to support the methods described above.

New processes for the production of polymeric dispersions with high solids content and low viscosity were developed, investigated and characterized. The specifications required for the desired application of paper coating, which constitutes one of the major innovative aspects of this thesis, requires in average particle sizes smaller than 200 nm. This particle size is significantly smaller than obtained in previous work in this area. The main objective of this project was to increase the solids content of existing products from ~50 to ~60 wt% while keeping the viscosity at low levels (< 1200 mPa•s at 20 s-1). In order to produce high solids content latexes with low viscosity, bimodal particle size distributions were resorted to. To obtain highest packing fraction, the small particle size population should be about 7 times smaller than the large particles, bringing the size of the small particles to less than 30 nm. Modified (micro)emulsion processes were developed in order to produce small particle size latex with reduced surfactant concentration and increased solids content. The large particle population was developed using a semi-batch emulsion polymerization process, simulating a product that is commercially available (~52 wt% solids content and viscosity of ~500 mPa•s at 20 s-1). To increase the solids content of this product up to 60 wt%, a second population of small particles was created using two approaches. In the first approach, the small particles were generated in situ using the modified (micro)emulsion approaches developed previously. This process resulted in latexes of ~ 60% solids content and viscosities lower than 500 mPa•s at 20 s-1. In the second approach, the second population of particles was created by the addition of seeds by using small cross-linked particles as pseudo inert-fillers. This process resulted in products with ~58% solids and viscosities lower than 1400 mPa•s at 20 s-1. The slightly decreased solids content and increased viscosity relative to the previous approaches is due to the difficulty in producing cross-linked seeds with particle sizes smaller than 30 nm at an acceptable concentration, causing deviations from ideal conditions. / Thesis (Ph.D, Chemical Engineering) -- Queen's University, 2011-05-03 13:58:44.22

In applied research, a lack of understanding of corner stall, i.e. the three-dimensional (3D) separation in the juncture of the endwall and blade corner region, which has limited the efficiency and the stability of compressors. Both Reynolds-averaged Navier-Stokes (RANS) and large eddy simulation (LES) still need to be calibrated for turbomachinery applications. In the fundamental research of the turbulent boundary layer (TBL), there are a lot of findings of the effects of curvature and pressure gradients, which also play an important role in physics of corner stall. The purpose of this thesis is (i) to carry out an experiment in a cascade, (ii) to gain a database that could be used to calibrate both RANS and LES, and (iii) to give some basic explanations of corner stall through investigating the TBL on the suction side at the mid-span which is more complex than those in the basic investigations but simpler than those in a real engine. A detailed and accurate experiment of 3D flow field through a linear compressor cascade has been set up. Experimental data were acquired for a Reynolds number of 3.82×10 ^5 based on blade chord and inlet flow conditions. Measurements have been achieved by hot-wire anemometry, pressure taps on blade and endwall, five-hole pressure probe, oil visualization, 2D particle image velocimetry (PIV),and two-component laser Doppler anemometry (LDA). An original and complete database was thus obtained. The TBL on the suction side at mid-span was investigated. The wall-normal negative pressure gradient restrains the separation, on the contrary to its influence in the corner stall. The streamwise adverse pressure gradient can be responsible for the development of Reynolds stresses. The remarkable phenomenon at measurement stations near the trailing edge of blade is that an inflection point occurs in each profile of the mean streamwise velocity. At this inflection point, the magnitudes of the Reynolds stresses reach their maximum values, and the direction of energy diffusion also changes. The velocity field in the corner stall was presented. Bimodal histograms of velocity exist in the experiment. The bimodal points mainly appear in the region around the mean interface of separated flow and non-separated flow. At a bimodal point the local two velocity components are non-independent from each other, due to the aperiodic interplay of two basic modes in the flow field. Two modes were proposed to interpret the physics of bimodal behaviour.

No description available.

Made available in DSpace on 2016-06-02T19:22:12Z (GMT). No. of bitstreams: 1 6335.pdf: 3771187 bytes, checksum: 3b53ad55024114e65e566f79ed09086b (MD5) Previous issue date: 2014-06-30 / Financiadora de Estudos e Projetos / The African sharptooth catfish Clarias gariepinus is a facultative air-breather with the ABO present in the 2nd and 3rd gill arches. Like other bimodal respirators, the African catfish presents cardiorespiratory adjustments to survive low PwO2. These regulations are modulated by gill O2 chemoreceptors monitoring the PwO2 and/or the PaO2. The present study analyzed the distribution, location and orientation of the branchial O2 chemoreceptors of C. gariepinus, and the role of these receptors on the cardiorespiratory function and the modulation of the heart autonomic control. C. gariepinus were divided into two experimental groups (Control GC, intact fish, and Experimental GD, with the 1st gill arches excised) and their cardiorespiratory variables were analyzed during graded hypoxia with and without access to the atmospheric air, internal and external NaCN injections and autonomic nervous system antagonists. During hypoxia without access to air, both GC and GD showed increases in the respiratory variables (fR, VT and &#119881; &#119866; ). However, the GD displayed higher values, since normóxia until severe hypoxia, to compensate the decrease in the EO2 resulting from the ablation of the 1st gill arches. Furthermore, the decrease in EO2 was responsible for the decrease in &#119881; O2 and, consequently, for the increase of the PcO2 from 61 mmHg (GC) to 79 mmHg (GD). The GD featured bradycardia similar to GC, however, their values were higher in all PwO2. In hypoxia with access to air, both groups showed decrease in the fR and VAMP in the mores hypoxic tensions. This was attributed to the increases in the fRA in GC which compensate the O2 uptake. However, the GC increases in fRA were attenuated by the excision of the 1st gill arches. Moreover, both groups developed bradycardia pre-RA (typical of aquatic respirator) and tachycardia post-RA (typical of aerial respirator). Internal and external NaCN injections caused bradycardia in both groups, while the internal injections increased the ventilator variables only in GC. Thus, it is concluded that C. gariepinus possess branchial O2 chemoreceptors modulating the fH distributed by all gill arches, monitoring both PwO2 and PaO2. Conversely, fR and VAMP are modulated by receptors located in the 1st gill arches monitoring exclusively the PaO2. Furthermore, NaCN internal injections elevated more the fRA in GC than the external ones, but with low effect on the fRA of GD. Thus, the fRA is primarily modulated by O2 chemoreceptors located in the 1st gill arches monitoring, predominantly, the PaO2. Injections blocking the autonomic system to the heart showed that the modulation of pre-AR bradycardia and post-AR tachycardia occurs due to variations in the vagal cholinergic tonus, which is elevated during bradycardia and reduced during tachycardia. The adrenergic tonus, in spite of the decrease in the post-AR, is lower than the cholinergic, suggests a "reflex adrenergic" arising from the peripheral vasoconstriction, important to optimize the perfusion of the ABOs. / O bagre-africano é um teleósteo de respiração bimodal com ABOs presentes nos 2os e 4os arcos branquiais. Como outros respiradores bimodais, o bagre-africano realiza ajustes cardiorrespiratórios para sobreviver em baixas PwO2. Estes são modulados por quimiorreceptores de O2 branquiais que monitoram a PwO2 e/ou PaO2. O presente estudo analisou a distribuição, localização e orientação dos quimiorreceptores de O2 branquiais de Clarias gariepinus, seu controle sobre a função cardiorrespiratória e a modulação do controle autonômico do coração. Para isto, C. gariepinus foi dividido em dois grupos (controle GC; experimental GD, com ablação do 1o par de arcos branquiais) e seus parâmetros cardiorrespiratórios foram analisados durante hipóxia sem e com acesso ao ar atmosférico, injeções internas e externas de NaCN e injeções antagonistas do sistema autonômico. Durante hipóxia sem acesso ao ar os GC e GD elevaram os parâmetros respiratórios (fR, VT e &#119881; &#119866; ). Porém, o GD apresentou-os superestimados desde normóxia, a fim de compensar a queda na extração de O2 (EO2) resultante da ablação dos 1os arcos branquiais. Além disso, o decréscimo da EO2 foi responsável pela diminuição da &#119881; O2 , e, consequentemente, pelo aumento da PcO2 de 61 mmHg para 79 mmHg, nos GC e GD, respectivamente. Ainda, o GD apresentou bradicardia semelhante ao GC, porém seus valores foram superestimados em todas as PwO2. Em hipóxia com acesso ao ar ambos os grupos demonstraram diminuição da fR e da VAMP nas últimas PO2 devido principalmente a aumentos da fRA no GC que compensaram a absorção de O2. Todavia, no GD o aumento da fRA foi atenuado pela ablação dos 1os arcos branquiais. Além disso, ambos os grupos apresentaram bradicardia pré-RA (típica de respirador aquático) e taquicardia pós-RA (típica de respirador aéreo). Injeções externas e internas de NaCN causaram bradicardia nos GC e GD, enquanto as injeções internas aumentaram os parâmetros ventilatórios apenas no GC. Desta forma, conclui-se que C. gariepinus apresenta quimiorreceptores de O2 branquiais, que modulam a fH, distribuídos por todos arcos branquiais e que monitoram tanto a PwO2 quanto a PaO2. Ao contrário, a modulação da fR e VAMP ocorre apenas por quimiorreceptores de O2 do 1o par de arcos branquiais e que monitoram apenas a PaO2. Ainda, injeções internas de NaCN elevaram mais a fRA no GC que as externas, porém tiveram pouco efeito sobre a fRA do GD. Assim, a fRA é modulada principalmente por quimiorreceptores de O2 dos 1os arcos branquiais que, predominantemente, monitoram a PaO2. Injeções bloqueadoras do sistema autonômico do coração de C. gariepinus demonstraram que a modulação tanto da bradicardia pré-RA quanto da taquicardia pós-RA ocorre pela variação do tônus vagal colinérgico, sendo este tônus elevado na bradicardia, porém reduzido na taquicardia. O tônus adrenérgico, apesar de diminuir pós-RA, esta queda é menor que a do tônus colinérgico, o que sugere um reflexo adrenérgico advindo da vasoconstrição periférica importante para os respiradores bimodais perfundirem melhor seus ABOs.