Vertebrates are the most prodigious vocalizing animals in existence, and the most diverse methods of acoustic communication among vertebrates can be found in the ocean. Relatively many teleost fish are gifted with the ability to communicate acoustically, and the family of serranidae often performs this as a function of the swim bladder. Epinephelus Guttatus (E. guttatus), or more commonly the red hind grouper, is equipped with a drum shaped swim bladder acting as a monopole under typical ocean conditions. This configuration allows for what is understood to be omnidirectional projection of tones approximately centered between 40 and 440 Hz and spanning anywhere from 40 to 200 Hz of bandwidth and modulation effects based on observed data provided by researchers. Prior studies on many other fish show correlation in acoustic communication profile with length, size and sexual identity. In the red hind, sexual dimorphism leads to an inherent female identity in all juvenile fish which converts to male according to environmental factors, recommending at least consistent organs across both sexes be assumed even if not in use. Much research has been performed on male fish vocalization in terms of spectral content. Communication in fish is a complex multi-modal process, with acoustic communication being important for many of the species, particularly those in the littoral regions of the worlds’ oceans. If identifying characteristics of the red hind vocalization can be isolated based on detection, classification, tracking and localizing methodologies, then these identifying characteristics may indeed lead to passive feature identification that allows for estimation of individual fish mass. Hypotheses based on vector, cyclostationary and classical tonal mechanics are presented for consideration. A battery of test data collection events, applying pre-recorded fish vocalizations to a geolocated undersea sound source were conducted. The results are supplied with the intent of validating hypothesis in a non-expert system manner that shows how a series of biological metrics may be assessed for detection, classification, localization and mass estimation for an individual vocalizing red hind grouper / Includes bibliography. / Dissertation (Ph.D.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection

Orientador: Reginaldo José Donatelli / Resumo: O bem-te-vi, Pitangus sulphuratus, é um dos pássaros mais comuns em quase todo o Brasil e considerado o mais popular deste país. Apresenta um comportamento altamente versátil e generalista, e encontra alimento facilmente em qualquer hábitat, tendo uma ampla distribuição geográfica, sendo encontrado do Texas ao sul da Argentina. No Brasil ocorrem quatro subespécies, que apresentam diferenciações nas cores da plumagem do dorso e do ventre. Inserido na Classe Aves, ordem Passeriformes, subordem Suboscines, tem o seu canto considerado inato, já que se desenvolve normalmente na ausência de um modelo auditivo. As aves Suboscines são bons modelos de análise vocal entre populações, já que quaisquer diferenças entre suas vocalizações não poderiam ser atribuídas aos padrões culturais de aprendizado. Considerando-se que o ambiente exerce uma seletividade na transmissão do som, indivíduos de uma mesma espécie podem apresentar diferenças vocais por estarem em ambientes diferentes; sendo assim, a localização pode exercer uma padronização ou diferenciação nas vocalizações das aves. Neste trabalho foram utilizadas 288 vocalizações de Pitangus sulphuratus, os quais foram classificados de acordo com sua sintaxe. Realizaram-se análises estruturais acústicas para a comparação e identificação de possíveis variações vocais entre as populações das subespécies presentes no Brasil e correlação com a altitude, latitude, longitude, bem como variáveis bioclimáticas (temperatura e precipitação). Foram id... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre

O conhecimento acerca das vocalizações dos odontocetos é extremamente heterogêneo e concentra-se principalmente nas espécies mantidas em cativeiro. Neste estudo, descrevo quantitativamente parte do repertório vocal dos golfinhos-rotadores, Stenella longirostris, que vivem ao redor do Arquipélago de Fernando de Noronha, subdividido nos seguintes elementos: assobios, grasnidos emitidos na faixa audível e grasnidos emitidos pelos machos durante a corte. Além disso, avaliei a influência tantos dos diferentes sistemas de gravação e análise de dados, quanto da escolha, interpretação e extração de variáveis por diferentes pesquisadores na variabilidade dos assobios e grasnidos emitidos por golfinhosrotadores. Para avaliar a variabilidade dos assobios desta população de golfinhos-rotadores, uma série de análises quantitativas foi conduzida. Nove variáveis foram extraídas de cada contorno de assobio selecionado aleatoriamente. Os valores médios observados foram muito similares àqueles reportados por OSWALD et al. (2003) para golfinhos-rotadores do Oceano Pacífico Tropical Oriental e apresentaram diferença significativa apenas em relação a freqüência máxima dos assobios. Com relação aos grasnidos, amostras foram selecionadas aleatoriamente e destas foram extraídas oito variáveis. Uma série de análises quantitativas foram então conduzidas para avaliar a variabilidade destas emissões. Os valores obtidos para cada uma das variáveis diferiram dos valores previamente reportados para a espécie, mas as razões para esta variação são ainda desconhecidas. Na busca de indícios sobre a função dos grasnidos no comportamento reprodutivo, eu dividi os grasnidos gravados em duas categorias: corte e não-corte. Grasnidos de corte foram aqueles sons gravados na presença de grupos de golfinhos compostos por uma ou duas fêmeas e por vários machos, engajados em comportamento de corte. Grasnidos de não-corte eram todos aqueles gravados em outros comportamentos ou situações. Os resultados obtidos mostraram diferenças significativas entre as duas categorias tanto nas análises univariadas, quanto na Análise Discriminante (AD), pelo menos quanto à duração e ao número de pulsos. Além disso, a AD mostrou que 98% das amostras foram corretamente classificadas nessas categorias. Estes resultados reforçam a idéia de que os grasnidos são usados em contextos sociais, especialmente em situações aflitivas e exaltam a importância da identificação do emissor dos sinais, assim como o uso de equipamentos de gravação de amplo espectro de freqüência. Por fim, realizei uma série de testes para avaliar a metodologia de gravação e análise de sons. Para isso, gravei as emissões utilizando dois sistemas distintos de gravação, ambos com taxa de amostragem de 48 kHz. Além disso, dois pesquisadores diferentes extraíram as variáveis de um mesmo grupo de amostras e os resultados obtidos para cada pesquisador, bem como para cada sistema foram comparados. Os resultados mostraram que, para os assobios, houve diferenças significativas entre as análises de cada pesquisador, quanto à às variáveis freqüência mínima, freqüência final e duração. Ao comparar os resultados obtidos pelos diferentes sistemas de gravação, notei que a duração apresentou diferenças significativas. Quanto aos grasnidos, notei uma diferença significativa no número de amostras de qualidade gravadas por cada um dos sistemas de gravação. Entretanto, as amostras gravadas em ambos os sistemas não apresentaram diferenças significativas em nenhuma das variáveis extraídas, tampouco foram observadas diferenças entre os diferentes pesquisadores. Acredito que o conhecimento gerado aqui contribuirá para uma melhor compreensão dos padrões de variação da espécie e servirá como base para uma série de outros estudos comparativos. / Knowledge on odontocete vocalizations is extremely heterogeneous and centered mainly in those species kept in captivity. In this study, I describe quantitatively part of the vocal repertoire of the spinner dolphin, Stenella longirostris, from Fernando de Noronha Archipelago, off Brazil, subdivided in the following elements: whistles, burst-pulses emitted in the aural range, and burst-pulses emitted by males during courtship. Furthermore, I evaluated the influence of different recording and analysis systems, and the choice, interpretation and extraction of the acoustic variables by different researchers in the variability of whistles and burst-pulses emitted by spinner dolphins. To evaluate the variability of whistles emitted by this population, a series of quantitative analyses was conducted. Nine variables were extracted from each randomly selected whistle contour. The mean values observed were very similar to those reported by OSWALD et al. (2003) for the spinner dolphins from Eastern Tropical Pacific and presented significant differences only in relation to the maximum frequency of the whistles. I extracted eight variables from randomly selected burst-pulsed sound samples. A series of quantitative analyses were conducted to evaluate the variability of these emissions. The values obtained from each of the variables differed from previously data reported for the species, but the reasons to this variation are still unknown. In the search of any signs of burstpulse functions in reproductive behaviors, I divided the recorded burst-pulses in two categories: court and no-court pulses. Court bursts were those sounds recorded in the presence of groups of dolphins composed by one or two females and several males, engaged in courtship behavior. No-court bursts were all those recorded in other behaviors or situations. The results showed significant differences between the two categories in univariate analysis and also in the Discriminant Function Analysis (DFA). The DFA also showed that 98% of the samples were correctly classified in these categories. These results reinforce the idea that burst-pulsed sounds are used in social contexts, specially in afflictive situations, and exalt the importance of identifying the signal emitter, as well as using wide frequency capable recording equipment. Finally, I performed a series of tests to evaluate the methodology of recording and sound analysis. To achieve this, I recorded the sound emissions using two different recording systems, both with sample rate of 48 kHz. Furthermore, two rsearchers extracted the variables of a group of samples, and the results from each researcher and each recording system were compared. Taking into account the whistles, the results showed that there were significant differences between the researchers on duration, minimum and final frequencies recorded. I compared the results obtained by different recording systems and noticed that the sound duration presented significant differences. With respect to burst-pulses, I noticed a significant difference in the number of samples with quality recorded by each of the recording systems. However, the samples recorded in both systems didn\'t show any difference in none of the extracted variables, nor there were observed differences between researchers. I believe that the knowledge produced here will contribute to the comprehension of variation patterns, and will also serve as a basis to further comparative studies.

Em anuros o canto de anúncio é um mecanismo primário de isolamento reprodutivo e possui relevância para taxonomia do grupo. O grupo de espécies Boana pulchella possui cerca de 40 espécies e tem seu monofiletismo indicado por evidencia molecular, abriga um subgrupo informal composto pelas formas de perto relacionadas à B. polytaenia, com 12 espécies. Destas, quase todas têm seus cantos de anúncio descritos, porem problemas nesses registros incluem baixo tamanho amostral, falta de variáveis relevantes e limitação e obsolescência das tecnologias de registro e analises. Tendo em vista a importância da vocalização para a taxonomia em anuros, e desta para diversas áreas da biologia em geral, é relevante e necessário uma avaliação/reavaliação acústica da identificação de várias populações e espécies do grupo de B. polytaenia para uma compreensão da sua riqueza e padrões de distribuição. Analisamos e comparamos dados acústicos de populações de B. cipoensis, provenientes de sua localidade tipo (Cipó) e da Serra da Canastra e de B. bandeirantes originárias de três populações. Também avaliamos os espécimes e cantos de populações de Atibaia (SP) e Passos (MG), com potencial de descrição de espécies novas. Os cantos de anúncio foram registrados com gravadores digitais e microfones direcionais; foram analisadas variáveis como duração do canto, pulsos por nota, duração dos pulsos, notas por canto, duração das notas, faixa de frequência dominante (mínima e máxima) e pico de frequência. Para determinações especificas, espécimes foram comparados com topotipos e com a descrição original, a partir de análises morfométricas. Testes estatísticos padrões foram utilizados para avaliar a eventual significância das diferenças nas variáveis acústicas e morfométricas analisadas. Resultados apontam para uma população isolada que possivelmente representa uma espécie nova na Serra da Mantiqueira, bem como caracteriza diversas populações de B. bandeirantes e B. polytaenia; também estende a 9 distribuição de B. beckeri para o Município de Passos (MG) e B. stenocephala para região da Serra da Canastra (MG); e descreve pela primeira vez o repertório acústico de B. cipoensis da Serra do Cipó, localidade tipo / In anurans the advertisement call is a primary mechanism of reproductivity isolation and has relevance for the groups taxonomy. The species group Boana pulchella has around 40 species and have their monophyletism indicate by molecular evidence, covers an informal subgroup composed by species close related to B. polytaenia, with 12 species. From which, almost all have advertisement calls described, although problems in these registers includes small sample size, lack of relevant variables and limitation and obsolesce of the technologies of register and analyses. In view of the importance of vocalization to anuran taxonomy, and that for a variety of areas of biology in general, it is relevant and necessary an evaluation/revaluation acoustic of the identity of many populations and species from the group B. polytaenia to a understanding of its richness and distribution patterns. We analyse and compare acoustic data from populations of B. cipoensis from its type locality (Cipó) and from Canastra Mountain Range and B. bandeirantes from three populations. We also evaluated populations from Atibaia (SP) and Passos (MG), with the potential to describe new species. The advertisement calls were registered with digital recorders and directional mics; variables were analysed such as call duration, pulses per note, pulse duration, notes per call, note duration, dominant frequency range (minimum and maximum) and peak frequency. To specific determinations species were compared with topotypes and with the original descriptions, through morphometric analyses. Standard statistical tests were used to evaluate the eventual significance of the difference on the acoustic and morphometric variables analysed. Results show an isolated population which possibly represents a new species on Mantiqueira Mountain Range, as well as characterizing many populations of B. bandeirantes and B. polytaenia; also extends the distribution of B. beckeri to the municipality of Passos (MG) and B. stenocephala to 11 Canastra Mountain Range region (MG); and describe for the first time the acoustic repertoire of B. cipoensis from Cipó Mountain Range, its type locality

Le Quetzal Resplendissant Pharomachrus mocinno est une espèce d’oiseau tropical considérée fortement menacée par la perte de son habitat due aux activités humaines. Le Quetzal Resplendissant joue un rôle important de disperseur de graines et constitue le centre de la culture maya passée et présente. Les recherches sur cette espèce couvrent plusieurs aspects de son histoire naturelle et de sa biologie. Néanmoins, à ce jour, il n’y a aucune description détaillée du comportement et de l’écologie acoustiques de cette espèce, condition préalable à une conservation efficace de l'espèce. L’objectif de cette thèse a été d’étudier tout particulièrement le comportement et l’écologie acoustique de P. mocino dans la forêt nuageuse du Guatemala. Une analyse détaillée des vocalisations de P. mocinno, incluant des expériences de propagation de ses vocalisations dans son habitat, a permis d’identifier deux types de vocalisations destinés à la communication à longue distance et deux autres types de vocalisations destinés à la communication à courte distance. La quantification des différences dans les vocalisations territoriales des deux sous-espèces de Quetzals Resplendissants, P. m. mocinno (partie nord de l’Amérique centrale et sud du Mexique) et P. m. costaricensis (sud de l'Amérique centrale) a révélé de nettes différences entre les sous-espèces, ce qui conforterait l'hypothèse d’espèces distinctes. L'observation de l'espèce dans la canopée dense est difficile et la manipulation des individus est controversée en raison de sa grande importance culturelle. De fait, un système acoustique automatique a été mis au point pour suivre l’espèce de manière non invasive. Le système s'est avéré efficace et a produit des résultats révélant des profils de suivis acoustiques en partie dépendants de variables environnementales. Enfin, la communauté acoustique des oiseaux à laquelle P. mocinno appartient a été analysée afin d’évaluer les interactions interspécifiques de compétition. Les recherches développées ici devraient aider aux décisions de conservation futures concernant le Quetzal Resplendissant et son habitat, la forêt nuageuse. Cette recherche montre également que l'écoacoustique peut constituer une stratégie utile pour aborder les problèmes d'écologie et de conservation dans les zones tropicales. / The Resplendent Quetzal Pharomachrus mocinno is a tropical bird considered in a high risk of danger. Degradation of its habitat caused by human activities is the principal menace. The Resplendent Quetzal is important as seed disperser and is the centre of the past and present Mayan culture. The available studies about the species have covered aspects of the natural history and biology. Nevertheless, the description of the acoustic behaviour and ecology, a prerequisite for the conservation of the species, was not available. The general aim of this PhD thesis was to investigate the acoustic behaviour and ecology of P. mocinno in the cloud forest of Guatemala. A detailed analysis of P. mocinno vocalizations, including propagation experiments of these vocalizations in its habitat, led to identify two vocalizations intended for long range, and two for short range communication. Quantification of acoustic parameters in territorial vocalizations of the two subspecies of the Resplendent Quetzal, P. m. mocinno (north part of Central America and Chiapas) and P. m. costaricensis (south part of Central America), revealed clear differences between the subspecies, that could support a species separation hypothesis. . The observation of the species in the dense canopy is difficult and manipulation of individuals is controversial due to its high cultural importance. Then, an automatic acoustic system was developed as a method to study the species in a non-invasive way. The system proved to be efficient and returned results that revealed acoustic patterns linked to environmental variables. Finally, the acoustic community of other bird species P. mocinno belongs to was analysed so that interspecific competition interactions could be assessed. The research here developed should help in future conservation decisions about the Resplendent Quetzal and its habitat, the cloud forest. This research also illustrates that ecoacoustics can be a valuable strategy to tackle ecology and conservation questions in tropical areas.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-07T20:27:42Z No. of bitstreams: 1 2010 - Andrea Cec?lia Sicotti Maas.pdf: 2297895 bytes, checksum: 356a05153807fe7b25a4fd3042b79964 (MD5) / Made available in DSpace on 2017-02-07T20:27:42Z (GMT). No. of bitstreams: 1 2010 - Andrea Cec?lia Sicotti Maas.pdf: 2297895 bytes, checksum: 356a05153807fe7b25a4fd3042b79964 (MD5) Previous issue date: 2010-12-16 / CNPq / Studies envolving bioacustic have strongly collaborated for understanding phylogenetics, ontogenetics, environmental and behavioural processes. Vocal communication is important for the bats for navigation and food attainment, nevertheless represents a key aspect for social interactions. Several studies have demonstrated characteristics of echolocation vocalizations and other types of audio signals. However, few studies have been described with respect to of distress calls. Among these studies have described the vocalizations of distress calls as being of animals in situations of extreme danger, such as when the animal is caught by a predator or even when it is contained by a researcher. Some authors have offered hypotheses to explain a general convergence in the structure of vocalizations produced by birds and mammals in similar behavioral contexts. However, there are few studies have been done on the distress calls in bats. The present general study's objective is to produce and analyze a database for bioacoustics in the context distress vocalizations emitted by bats, analyze and describe the acoustic properties of distress vocalizations in bats of the subfamily Stenodermatinae. Data collection investigations have been carried out in five different locations within the southeast region of Brazil. For this study, were recorded vocalizations of bats in subfamily Stenodermatinae: Artibeus cinereus, Artibeus fimbriatus, A. planirostris, A. obscurus, A. lituratus, Platyrrhinus lineatus, P. recifinus and Vampyressa pusilla. The vocalizations were analyzed using the analysis system Raven v1.3. For analysis, we selected 30 calls of each individual. The following parameters were measured: Types of signs, bands tonal, duration of the signal, and interval of Pre-pulse, interval of post-pulse, minimum frequency, maximum frequency, initial frequency, maximum frequency and peak frequency. For a description of the acoustic parameters were averaged, standard deviation and coefficient of variation values of each of the measured parameters of vocalizations for each species. Through the analysis, we identify two basic types of notes for this noise present in the calls of distress among the species. The results presented here showed that the acoustic parameters corroborate other work in this same context, ie: distress vocalizations of the bat species studied may be the motivation of aggression, as the code calling the motivational structure, characterized in general for they present low frequency, in wide bands and loaded of noises. / As pesquisas com bioac?stica t?m colaborado na compreens?o de processos filogen?ticos, ontogen?ticos, ecol?gicos e comportamentais. Para os morcegos, a comunica??o vocal ? importante para navega??o e obten??o de alimentos, al?m de ser um aspecto chave nas intera??es sociais. V?rios estudos t?m demonstrado caracter?sticas de vocaliza??es de ecolocaliza??o e outros tipos de sinais aud?veis. Por?m, poucos estudos t?m sido descritos com rela??o ?s chamadas de distress. Dentre esses estudos t?m-se descrito as vocaliza??es de distress como sendo chamadas de animais em situa??es de extremo perigo, tais como quando esse animal ? capturado por um predador ou mesmo quando ? contido por um pesquisador. Alguns autores t?m apresentado hip?teses para explicar uma converg?ncia geral na estrutura das vocaliza??es produzidas por p?ssaros e mam?feros em contextos comportamentais similares. H?, entretanto, poucos estudos sendo desenvolvidos sobre as chamadas de distress em morcegos. O presente estudo tem como objetivo geral ? elabora??o e an?lise de um banco de dados bioac?sticos para vocaliza??es no contexto distress, emitidas por morcegos filostom?deos, analisar e descrever as propriedades ac?sticas das vocaliza??es de distress em morcegos da subfam?lia Stenodermatinae. As coletas dos dados bioac?sticos foram realizadas em cinco locais diferentes dentro da regi?o sudeste do Brasil. Para o presente estudo, foram gravadas vocaliza??es de morcegos da Subfam?lia Stenodermatinae: Artibeus cinereus, Artibeus fimbriatus, A. planirostris, A. obscurus, A. lituratus, Platyrrhinus lineatus, P. recifinus e Vampyressa pusilla. As vocaliza??es foram analisadas utilizando o sistema de an?lise Raven v1.3. Para an?lise, foram selecionadas 30 chamadas de cada indiv?duo. Os seguintes par?metros foram mensurados: Tipos de Sinais, bandas tonais, dura??o do sinal, Intervalo do Pr?-pulso, Intervalo do P?s-pulso, freq??ncia m?nima, freq??ncia m?xima, freq??ncia inicial, freq??ncia m?xima e freq??ncia pico. Para a descri??o dos par?metros ac?sticos foram calculados a m?dia, o desvio padr?o e o coeficiente de varia??o dos valores obtidos de cada um dos par?metros medidos das vocaliza??es para cada esp?cie. Atrav?s das an?lises, pudemos identificar dois tipos b?sicos de notas para esta emiss?o sonora presente nas chamadas de distress dentre as esp?cies estudadas. Os resultados aqui apresentados mostraram que os par?metros ac?sticos corroboram com outros trabalhos neste mesmo contexto, ou seja: as vocaliza??es de distress das esp?cies de morcegos estudadas podem representar a motiva??o de agressividade, conforme o c?digo de estrutura motivacional da vocaliza??o, caracterizadas em geral por apresentarem baixa frequ?ncia, em bandas larga e carregada de ru?dos.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-11-08T17:53:03Z No. of bitstreams: 1 2016 - Israel de S? Maciel.pdf: 3184590 bytes, checksum: 1d079e88bc7d2ff9256dc558436b7cd3 (MD5) / Made available in DSpace on 2017-11-08T17:53:03Z (GMT). No. of bitstreams: 1 2016 - Israel de S? Maciel.pdf: 3184590 bytes, checksum: 1d079e88bc7d2ff9256dc558436b7cd3 (MD5) Previous issue date: 2016-02-02 / CAPES / Pinnipeds produce a variety of underwater sounds that are often related to reproductive activities and social interactions. In addition, several species of seals provide evidence of vocalizations with geographical and individual variations. Most of bioacoustics researches with seals studied the terrestrial environment repertoire using linear analysis. However, some characteristics of vocalizations can not be explained with linear analysis only or taking into account only one part of the animal's life. The nonlinear phenomena (NLP) present in vocalizations (sideband, harmonic, sub-harmonic, biphonation, jump of frequency and deterministic chaos) are still poorly studied. Given the importance of this phenomena in vocalizations to individual recognition, especially in social mammals, was studied the underwater vocalizations of Antarctic seals in order to know their vocal repertoire and their NLP as well as discuss their possible function. In 2013, Brazilian Navy conducted acoustic recordings on Half Moon Island. In this collection were recorded 128GB of sound files between 12th and 30th of November. A total of 18 days of uninterrupted collections, producing 7,448 files of 3 minutes each. These recordings were taken at the presence of seals to Lobodontini Tribe. A total of 15 types of vocalizations were found in the period between days 12-16/November. A pattern in the vocalizations types L, K and E, arranged in sequence K, L and E, respectively, was observed in all seal recordings. All files presented biphonations, jumps of frequency, harmonics, sidebands and chaos in nine types of vocalizations (A, C, E, F, K, L, M, N and O). The nonlinearities were abundant on all files, being present in 75.56% (N = 1829) of vocalizations. Deterministic chaos was most common, present in 53.86% of non-linear features and 43.08% of vocalizations in general. Taking into account only the tonal vocalizations, 88.85% of vocalizations were nonlinear. The repetition of the KLE pattern also displays a recognition function. The NLP, mainly deterministic chaos, had high incidence in the studied vocalizations indicating that this type has an important role in communication. Furthermore, it seems to belong to the acoustic pattern of Antarctic?s Lobodontines. / Pin?pedes produzem uma variedade de sons subaqu?ticos que frequentemente s?o relacionados com atividades reprodutivas e intera??es sociais. Diversas esp?cies de focas apresentam evid?ncias de vocaliza??es com varia??es geogr?ficas e individuais. A maioria dos trabalhos com focas estudou o repert?rio em ambiente terrestre e com olhares para as an?lises lineares. Entretanto, algumas caracter?sticas das vocaliza??es n?o podem ser explicadas apenas com an?lises lineares ou levando em conta apenas uma parte da vida do animal. Os fen?menos n?o lineares (FNLs) presentes em vocaliza??es (banda lateral, harm?nico, sub-harm?nico, bifona??o, pulos de frequ?ncia e caos determin?stico) ainda s?o pouco estudados. Dada a import?ncia deste fen?meno nas vocaliza??es para o reconhecimento individual, principalmente em mam?feros sociais, este trabalho buscou estudar as vocaliza??es subaqu?ticas de focas ant?rticas, visando conhecer seu repert?rio vocal e seus FNLs, bem como discutir a poss?vel fun??o dos mesmos. Em 2013, a Marinha do Brasil realizou grava??es ac?sticas na Ilha Meia Lua na Ant?rtica. Nesta coleta foram registrados 128GB de arquivos de som nos dias entre 12 e 30 de novembro, totalizando 7.448 arquivos de 3 minutos cada. Nestas grava??es foi constatada a presen?a de focas pertencentes ? Tribo Lobodontini. Contatou-se tamb?m a presen?a de 15 tipos de vocaliza??es no per?odo entre os dias 12 a 16/novembro. Em todos os dias que houve grava??es de focas foi observado um padr?o nos tipos L, K e E, organizados na sequ?ncia K, L e E, respectivamente. Ap?s a inspe??o visual de todos os arquivos, foram encontrados pulos de frequ?ncia, bifona??es, harm?nicos, bandas laterais e caos determin?stico em nove tipos de vocaliza??es (A, C, E, F, K, L, M, N e O). As n?o linearidades foram abundantes em todos os arquivos, estando presentes em 75,56% (N=1829) das vocaliza??es. O caos determin?stico foi o mais comumente observado, estando presente em 53,86% das fei??es n?o lineares e 43,08% das vocaliza??es em geral. Levando em conta apenas as vocaliza??es tonais, 88,85% das vocaliza??es foram n?o lineares. A repeti??o do padr?o KLE parece apresentar uma fun??o de reconhecimento. Os FNLs, principalmente os do tipo caos determin?stico, estiveram muito presentes nas vocaliza??es estudadas, indicando que este tipo de emiss?o possui uma fun??o importante na comunica??o. Al?m disso, estes parecem pertencer ao repert?rio ac?stico padr?o de Lobodontines ant?rticos.

This thesis investigates aspects of the ecology, morphology, bioacoustics, genetic relatedness, biogeography and taxonomy of cicadas in the Pauropsalta annulata Goding and Froggatt species complex, across their entire eastern Australian geographical distribution. These cicadas seem to emerge at the same sites annually, although the exact duration of their life cycle is not known. They are wary and mobile insects, with individuals generally taking flight at the first sign of disturbance, which makes them challenging to record, capture and observe. The males produce distinctive calling songs that have a consistent rhythmic structure, which is critically important for attracting females. In most cases, the songs are biphasic, with a penetrating introductory or "buzzing" component that apparently functions in mate attraction and a strongly structured cueing or "lilting" component for mate localisation. Initially Pauropsalta annulata was thought to comprise a single species with some degree of geographically structured variation in its calling song. Consequently, various "song types" had been recognised, but their precise relationship to one another had never been investigated. Therefore the structure of their calling songs were compared statistically across individuals of three song types, and this revealed four discrete clusters that were demonstrated to be independent of one another and consistent in their calling song structure. Plotting the geographical distributions of these cicadas revealed that each of the P. annulata song types is independently distributed geographically, with areas of overlap that are relatively small. Calling song structure is consistent for each song type across extensive geographical space and this structure holds even into areas of sympatry. One song type showed consistent differences from the others in male genitalia structure, and female ovipositor length differs significantly among three of the other song types. Each song type was found to be strongly associated with a small number of tree species and these associations are maintained into areas of sympatry. The spatial ecological and morphological comparisons in song structure, plant associations and morphology made in this study demonstrate that the P. annulata song types actually represent a complex of cryptic species. Two of the song types appear to intergrade in areas of sympatric overlap in terms of calling songs, plant associations and morphology, and thus represent subspecies as defined in population genetics terms. The components of the male calling song were then investigated to determine their specific functions and thus how they could delimit species boundaries. Significant differences were found in dominant song frequency between three of the four species, and may contribute to differential mate attraction. Both components of the song of each species have the same dominant frequency. Consistent differences were also evident, among species and subspecies, in the rhythmic structure of the "lilting" component of the male calling song. This component contains repeated phrases and each one of these provides a cue to which the conspecific female may respond. Her response is timed for the brief silent interval between the phrases. At this point the calling song becomes a duet, which enables the male to locate the female, as he actively searches for her on the surrounding branches at this stage. The calling song is discussed in terms of random mating within gene pools of these cicadas and in terms of its role as part of their broader fertilisation mechanism. A molecular analysis of the P. annulata species complex was performed to examine the phylogenetic relationships across 12 species and four subspecies defined in this study, and estimate divergence times within the group. Individual specimens were sampled widely across the geographical distributions of the species and subspecies where possible to account for genetic variation across space. DNA sequences from two loci were amplified: mitochondrial CO1 ("barcoding region") and a large intron from the dynamin nuclear gene. Separate phylogenies were reconstructed for each locus using maximum parsimony procedures and Bayesian posterior sampling with implementation of a relaxed molecular clock. The phylogenies from both genes provided strong support for the monophyly of the P. annulata species complex, and nine of the species were monophyletic based on the CO1 gene. The remaining three emerged non-monophyletic. Based on a clock calibration of 0.0165s/s/myr, the monophyletic clades represented by extant P. annulata species diverged about 4.5-8.0 million years ago. Those species that emerged non-monophyletic had shallower divergences, with the exception of one species, which exhibited haplotype diversity that conferred up to 13.2% sequence divergence between allopatric populations in CO1. Dynamin produced a broadly similar phylogenetic pattern to that of CO1, but the relationships among individuals across the species and subspecies that emerged non-monophyletic differed substantially. This lack of congruence between the two genomes, in combination with the dominance of internal haplotypes in both loci, indicates an overall pattern of deep coalescence rather than interspecific hybridization. Therefore the molecular data do not provide an alternative definition of species limits in the P. annulata species complex, despite some emerging non-monophyletic in this analysis. Sound interpretation of the phylogenetic pattern discovered here would not have been possible without the acoustic, ecological and geographical investigations on species limits that preceded this work. To determine what biological and climatic factors influence the present day distributions of these cicadas, the distributions of two of the most closely related species in the P. annulata species complex were compared with the distributions of the tree species with which each is mainly associated. Because a large part of their life cycle is subterranean, soil texture, pH, electrical conductivity and force required for surface penetration were compared across sites where each of the cicadas occur in sympatry and allopatry. Finally, the influence of temperature and rainfall variables were investigated by testing 'predicted distribution' models (formed using positive distribution records) against negative records for both sets of variables, individually and in combination. The results show that the extent of the distribution of the cicada species is substantially less than that of the associated tree species. The geographical distributions of one of the species may be influenced more by rainfall, or a combination of temperature and rainfall, whereas the other species appears to be more influenced by temperature alone. Both species tolerate soils with a wide range of pH levels, electrical conductivity and forces required for surface penetration. They both showed a strong association with soils that had a silt loam texture, with only few records from sandy soils. However, none of the soils sampled where the cicadas occurred were heavy clays, which suggests that the physical properties of such soils may provide an unsuitable environment for the nymphal stages of the life cycle of these particular cicadas. The resolution of species limits within the P. annulata species complex allowed the redescription of Pauropsalta annulata Goding and Froggatt sensu stricto and the description of 11 new species belonging to the P. annulata species group, all from eastern Australia. Two of these species comprise two subspecies each, also all new. New distribution records and calling song data are documented for the allied species, P. ayrensis Ewart, which is redescribed to include the characters newly recognised in the present study as significant taxonomically with respect to Pauropsalta cicadas. The treatment includes comprehensive descriptions of the morphology and calling songs of the species and subspecies, and separate descriptive keys are provided for both sets of characters. The new taxa comprise P. artatus sp. nov., P. corymbiae sp. nov., P. decorus sp. nov., P. graniticus sp. nov., P. inversus inversus subsp. nov., P. i. laboris subsp. nov., P. notialis notialis subsp. nov., P. notialis incitatus subsp. nov., P. simplex sp. nov., P. subtropicus sp. nov, P. torrensis sp. nov. and P. tremulus sp. nov. Areas of hybridization between P. n. notialis subsp. nov. and P. n. incitatus subsp. nov. are also documented, together with their calling songs and morphology, which justifies their subspecific status. The P. inversus subspecies are allopatric, but consistently differ in the duration between phrases of the calling song. Finally, the results and conclusions are amalgamated into a critical reassessment of what defines species limits and the most appropriate approaches to investigating species limits in sexual organisms. Some historical discussions are revisited, such as the question of the reality of species and how species are perceived under the premises of neoDarwinism. The realism of species demands that species limits are most realistically defined in terms of their fertilisation mechanism, for this delimits the gene pool and thus the distribution of adaptations (the calling song of cicadas, for example).

Le suivi des populations animales et végétales nous a amené à constater une perte importante de la diversité biologique. Les objectifs de la Convention sur la diversité biologique à atteindre pour 2010 sous-tendent la poursuite détaillée de ce suivi à l’échelle mondiale (CBD 2000). Cependant, il est difficile d’avoir une perception d’ensemble de la biodiversité d’un territoire, car les écosystèmes sont des entités dynamiques et évolutives, dans l’espace et dans le temps. Le choix d’un indicateur relevant de l’ensemble des ces caractéristiques devient donc primordial, bien qu’il s’agisse d’une tâche laborieuse. Ce projet propose d’utiliser la bioacoustique comme indicateur environnemental pour faire le suivi des espèces animales en milieu tropical. Afin de faire un suivi à une échelle régionale de la biodiversité, et ce, dans l’un des biomes les plus menacés de la planète, soit celui de la Mata Atlântica brésilienne, ce projet de recherche a comme objectif général de démontrer qu’il est possible d’associer la biophonie (événements sonores), à des événements biologiques (la richesse spécifique animale) en quantifiant des événements sonores (à l’aide des chants produits par les oiseaux, les insectes chanteurs de même que par les anoures) et en tentant de les associer aux fluctuations de la biodiversité. En plus de répondre à cet objectif général, trois objectifs spécifiques ont été définis : 1) comparer la biophonie et l’anthropophonie de milieux soumis à différents niveaux d’anthropisation ou de conservation afin d’évaluer l’impact anthropique sur le milieu, 2) évaluer la variabilité spatiale de la biodiversité, de même que 3) sa variabilité temporelle. Les résultats ont démontré que la biophonie est représentative de la biodiversité d’un milieu, et ce, même dans des conditions de biodiversité maximale puisqu’il existe une très forte relation entre les deux variables. De plus, les résultats révèlent une différence significative dans le ratio anthropophonie/biophonie de milieux soumis à différents niveaux de protection du territoire. La différenciation d’indices de puissance relative (dB/kHz) nous indique également l’importance de la variabilité spatiale et temporelle de la biodiversité, et par conséquent, l’importance de faire le suivi des espèces dans divers milieux et à diverses périodes afin d’obtenir une vision adéquate de la biodiversité régionale. / Recent monitoring of plant and animal populations has led us to observe a significant loss of global biodiversity. The objectives of the Convention on Biological Diversity for 2010 are to encourage environmental monitoring worldwide (CBD 2000). However, it is difficult to have an adequate portrait of the overall biodiversity of an area, because ecosystems are dynamic and evolving in both space and time. The choice of an indicator is therefore essential. This project proposes to use bioacoustics as an environmental indicator to monitor the animal biodiversity in tropical areas, in one of the most threatened biomes on the planet, the Mata Atlantica in Brazil. Our aim is to demonstrate that it is possible to relate biophony (sound events) to biological events (punctual animal species richness), i.e. to quantify songs produced by birds, insects and anurans singers and associate them to changes in biodiversity. In addressing this overall goal, three specific objectives were put forward: 1) compare the biophony and anthropophony of different landscapes, subjected to different levels of human occupation and conservation, in order to assess the human impact on the environment, 2) assess the spatial variability of biodiversity, as well as 3) its temporal variability. Results showed that biophony is representative of the biodiversity of an area, even under conditions of maximum biodiversity such as found in Brazil because there is a very strong positive relationship between these two variables. In addition, the results show a significant difference in the ratio anthropophony/biophony in environments subject to different human impacts. Disparate indices of relative power (dB / kHz) also reveal the importance of spatial and temporal variability of biodiversity, and therefore the importance of monitoring biophony in different environments and at different times to obtain an adequate portrait of a region’s biodiversity.

This thesis investigates aspects of the ecology, morphology, bioacoustics, genetic relatedness, biogeography and taxonomy of cicadas in the Pauropsalta annulata Goding and Froggatt species complex, across their entire eastern Australian geographical distribution. These cicadas seem to emerge at the same sites annually, although the exact duration of their life cycle is not known. They are wary and mobile insects, with individuals generally taking flight at the first sign of disturbance, which makes them challenging to record, capture and observe. The males produce distinctive calling songs that have a consistent rhythmic structure, which is critically important for attracting females. In most cases, the songs are biphasic, with a penetrating introductory or "buzzing" component that apparently functions in mate attraction and a strongly structured cueing or "lilting" component for mate localisation. Initially Pauropsalta annulata was thought to comprise a single species with some degree of geographically structured variation in its calling song. Consequently, various "song types" had been recognised, but their precise relationship to one another had never been investigated. Therefore the structure of their calling songs were compared statistically across individuals of three song types, and this revealed four discrete clusters that were demonstrated to be independent of one another and consistent in their calling song structure. Plotting the geographical distributions of these cicadas revealed that each of the P. annulata song types is independently distributed geographically, with areas of overlap that are relatively small. Calling song structure is consistent for each song type across extensive geographical space and this structure holds even into areas of sympatry. One song type showed consistent differences from the others in male genitalia structure, and female ovipositor length differs significantly among three of the other song types. Each song type was found to be strongly associated with a small number of tree species and these associations are maintained into areas of sympatry. The spatial ecological and morphological comparisons in song structure, plant associations and morphology made in this study demonstrate that the P. annulata song types actually represent a complex of cryptic species. Two of the song types appear to intergrade in areas of sympatric overlap in terms of calling songs, plant associations and morphology, and thus represent subspecies as defined in population genetics terms. The components of the male calling song were then investigated to determine their specific functions and thus how they could delimit species boundaries. Significant differences were found in dominant song frequency between three of the four species, and may contribute to differential mate attraction. Both components of the song of each species have the same dominant frequency. Consistent differences were also evident, among species and subspecies, in the rhythmic structure of the "lilting" component of the male calling song. This component contains repeated phrases and each one of these provides a cue to which the conspecific female may respond. Her response is timed for the brief silent interval between the phrases. At this point the calling song becomes a duet, which enables the male to locate the female, as he actively searches for her on the surrounding branches at this stage. The calling song is discussed in terms of random mating within gene pools of these cicadas and in terms of its role as part of their broader fertilisation mechanism. A molecular analysis of the P. annulata species complex was performed to examine the phylogenetic relationships across 12 species and four subspecies defined in this study, and estimate divergence times within the group. Individual specimens were sampled widely across the geographical distributions of the species and subspecies where possible to account for genetic variation across space. DNA sequences from two loci were amplified: mitochondrial CO1 ("barcoding region") and a large intron from the dynamin nuclear gene. Separate phylogenies were reconstructed for each locus using maximum parsimony procedures and Bayesian posterior sampling with implementation of a relaxed molecular clock. The phylogenies from both genes provided strong support for the monophyly of the P. annulata species complex, and nine of the species were monophyletic based on the CO1 gene. The remaining three emerged non-monophyletic. Based on a clock calibration of 0.0165s/s/myr, the monophyletic clades represented by extant P. annulata species diverged about 4.5-8.0 million years ago. Those species that emerged non-monophyletic had shallower divergences, with the exception of one species, which exhibited haplotype diversity that conferred up to 13.2% sequence divergence between allopatric populations in CO1. Dynamin produced a broadly similar phylogenetic pattern to that of CO1, but the relationships among individuals across the species and subspecies that emerged non-monophyletic differed substantially. This lack of congruence between the two genomes, in combination with the dominance of internal haplotypes in both loci, indicates an overall pattern of deep coalescence rather than interspecific hybridization. Therefore the molecular data do not provide an alternative definition of species limits in the P. annulata species complex, despite some emerging non-monophyletic in this analysis. Sound interpretation of the phylogenetic pattern discovered here would not have been possible without the acoustic, ecological and geographical investigations on species limits that preceded this work. To determine what biological and climatic factors influence the present day distributions of these cicadas, the distributions of two of the most closely related species in the P. annulata species complex were compared with the distributions of the tree species with which each is mainly associated. Because a large part of their life cycle is subterranean, soil texture, pH, electrical conductivity and force required for surface penetration were compared across sites where each of the cicadas occur in sympatry and allopatry. Finally, the influence of temperature and rainfall variables were investigated by testing 'predicted distribution' models (formed using positive distribution records) against negative records for both sets of variables, individually and in combination. The results show that the extent of the distribution of the cicada species is substantially less than that of the associated tree species. The geographical distributions of one of the species may be influenced more by rainfall, or a combination of temperature and rainfall, whereas the other species appears to be more influenced by temperature alone. Both species tolerate soils with a wide range of pH levels, electrical conductivity and forces required for surface penetration. They both showed a strong association with soils that had a silt loam texture, with only few records from sandy soils. However, none of the soils sampled where the cicadas occurred were heavy clays, which suggests that the physical properties of such soils may provide an unsuitable environment for the nymphal stages of the life cycle of these particular cicadas. The resolution of species limits within the P. annulata species complex allowed the redescription of Pauropsalta annulata Goding and Froggatt sensu stricto and the description of 11 new species belonging to the P. annulata species group, all from eastern Australia. Two of these species comprise two subspecies each, also all new. New distribution records and calling song data are documented for the allied species, P. ayrensis Ewart, which is redescribed to include the characters newly recognised in the present study as significant taxonomically with respect to Pauropsalta cicadas. The treatment includes comprehensive descriptions of the morphology and calling songs of the species and subspecies, and separate descriptive keys are provided for both sets of characters. The new taxa comprise P. artatus sp. nov., P. corymbiae sp. nov., P. decorus sp. nov., P. graniticus sp. nov., P. inversus inversus subsp. nov., P. i. laboris subsp. nov., P. notialis notialis subsp. nov., P. notialis incitatus subsp. nov., P. simplex sp. nov., P. subtropicus sp. nov, P. torrensis sp. nov. and P. tremulus sp. nov. Areas of hybridization between P. n. notialis subsp. nov. and P. n. incitatus subsp. nov. are also documented, together with their calling songs and morphology, which justifies their subspecific status. The P. inversus subspecies are allopatric, but consistently differ in the duration between phrases of the calling song. Finally, the results and conclusions are amalgamated into a critical reassessment of what defines species limits and the most appropriate approaches to investigating species limits in sexual organisms. Some historical discussions are revisited, such as the question of the reality of species and how species are perceived under the premises of neoDarwinism. The realism of species demands that species limits are most realistically defined in terms of their fertilisation mechanism, for this delimits the gene pool and thus the distribution of adaptations (the calling song of cicadas, for example).