Background: Re-vaccination against canine adenovirus (CAV) is performed in ≤3-year-intervals but its necessity is unknown. The study determined anti-CAV antibodies within 28 days of re-vaccination and factors associated with the absence of antibodies and vaccination response. Methods: Ninety-seven healthy adult dogs (last vaccination ≥12 months) were re-vaccinated with a modified live CAV-2 vaccine. Anti-CAV antibodies were measured before vaccination (day 0), and after re-vaccination (day 7, 28) by virus neutralization. A ≥4-fold titer increase was defined as vaccination response. Fisher’s exact test and multivariate regression analysis were performed to determine factors associated with the absence of antibodies and vaccination response. Results: Totally, 87% of dogs (90/97; 95% CI: 85.61–96.70) had anti-CAV antibodies (≥10) before re-vaccination. Vaccination response was observed in 6% of dogs (6/97; 95% CI: 2.60–13.11). Time since last vaccination (>3–5 years, OR = 9.375, p = 0.020; >5 years, OR = 25.000, p = 0.006) was associated with a lack of antibodies. Dogs from urban areas were more likely to respond to vaccination (p = 0.037). Conclusion: Many dogs had anti-CAV pre-vaccination antibodies, even those with an incomplete vaccination series. Most dogs did not respond to re-vaccination. Based on this study, dogs should be re-vaccinated every 3 years or antibodies should be determined.

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The UK building stock accounts for about half of all energy consumed in the UK. A large portion of the energy is consumed by nondomestic buildings. Offices and retail are the most energy intensive typologies within the nondomestic building sector, typically accounting for over 50% of the nondomestic buildings’ total energy consumption. Heating, ventilating and air conditioning (HVAC) systems are the largest energy end use in the nondomestic sector, with energy consumption close to 50% of total energy consumption. Different HVAC systems have different energy requirements when responding to the same building heating and cooling demands. On the other hand, building heating and cooling demands depend on various parameters such as building fabrics, glazing ratio, building form, occupancy pattern, and many others. HVAC system energy requirements and building energy demands can be determined by mathematical modelling. A widely accepted approach among building professionals is to use building energy simulation tools such as EnergyPlus, IES, DOE2, etc. which can analyse in detail building energy consumption. However, preparing and running simulations in such tools is usually very complicated, time consuming and costly. Their complexity has been identified as the biggest obstacle. Adequate alternatives to complex building energy simulation tools are regression models which can provide results in an easier and faster way. This research deals with the development of regression models that enable the selection of HVAC systems for office buildings. In addition, the models are able to predict annual heating, cooling and auxiliary energy requirements of different HVAC systems as a function of office building heating and cooling demands. For the first part of the data set development used for the regression analysis, a data set of office building simulation archetypes was developed. The four most typical built forms (open plan sidelit, cellular sidelit, artificially lit open plan and composite sidelit cellular around artificially lit open plan built form) were coupled with five types of building fabric and three levels of glazing ratio. Furthermore, two measures of reducing solar heat gains were considered as well as implementation of daylight control. Also, building orientation was included in the analysis. In total 3840 different office buildings were then further coupled with five different HVAC systems: variable air volume system; constant air volume system; fan coil system with dedicated air; chilled ceiling system with embedded pipes, dedicated air and radiator heating; and chilled ceiling system with exposed aluminium panels, dedicated air and radiator heating. The total number of models simulated in EnergyPlus, in order to develop the input database for regression analysis, was 23,040. The results clearly indicate that it is possible to form a reliable judgement about each different HVAC system’s heating, cooling and auxiliary energy requirements based only on office building heating and cooling demands. High coefficients of determination of the proposed regression models show that HVAC system requirements can be predicted with high accuracy. The lowest coefficient of determination among cooling regression models was 0.94 in the case of the CAV system. HVAC system heating energy requirement regression models had a coefficient of determination above 0.96. The auxiliary energy requirement models had a coefficient of determination above 0.95, except in the case of chilled ceiling systems where the coefficient of determination was around 0.87. This research demonstrates that simplified regression models can be used to provide design decisions for the office building HVAC systems studied. Such models allow more rapid determination of HVAC systems energy requirements without the need for time-consuming (hence expensive) reconfigurations and runs of the simulation program.

Obesity is considered to be one of the diseases related to the change in the lifestyle, leading to increased incidence of myocardial infarction, type 2 diabetes mellitus, hypertension and other diseases. Due to the progressively increasing prevalence of obesity in adulthood, prevention of obesity has to start in childhood and adolescent, in which also increase in prevalence of obesity was found. Actual prevalence of overweight and obesity was tested in this thesis by different standards (5. NAS, WHO, IOTF, CDC) in Czech children population in age of 6.5 - 7.5 years in 2013. Its trend since 1951 was evaluated. Since 2008 maintaining of the values in all categories at the constant level was found. The role of risk factors assessed by personal, family and school questionnaires in overweight prevalence was tested. The most important factors were diet, exercise and family factors. Increased weight/height ratio (WHtR) marker of adipose tissue was found in the category of normal weight children also and it shows the link with above-mentioned risk factors of diet, exercise and family prediction. Key words Obesity, Overweight, Prevalence, Childhood, BMI, 5. NAS, WHO, IOTF, CDC, COSI, WHtR, Waist circumference, Risk factors

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The manufacturing of connected and automated vehicles (CAVs) is happening and they are aiming at providing an efficient, safe, and seamless driving experience. This is done by offering automated driving together with wireless communication to and from various objects in the surrounding environment. How automated the vehicle is can be classified from level 0 (no automation at all) to level 5 (fully automated). There is many potential attack vectors of CAVs for attackers to take advantage of and these attack vectors may change depending on what level of automation the vehicle have. There are some known vulnerabilities of CAVs where the security has been breached, but what is seemed to be lacking in the academia in the field of CAVs is a place where the majority of information regarding known attack vectors and cyber-attacks on those is collected. In addition to this the attack vectors may be analyzed for each level of automation the vehicles may have. This research is a systematic literature review (SLR) with three stages (planning, conducting, and report) based on literature review methodology presented by Kitchenham (2004). These stages aim at planning the review, finding articles, extracting information from the found articles, and finally analyzing the result of them. The literature review resulted in information regarding identified cyberattacks and attack vectors the attackers may use as a path to exploit vulnerabilities of a CAV. In total 24 types of attack vectors were identified. Some attack vectors like vehicle communication types, vehicle applications, CAN bus protocol, and broadcasted messages were highlighted the most by the authors. When the attack vectors were analyzed together with the standard of ‘Levels of Driving Automation’ it became clear that there are more vulnerabilities to consider the higher level of automation the vehicle have. The contributions of this research are hence (1) a broad summary of attack vectors of CAVs and (2) a summary of these attack vectors for every level of driving automation. This had not been done before and was found to be lacking in the academia.

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<p dir="ltr">Cellular-Vehicle to Everything or C-V2X refers to vehicles connected to their surroundings using cellular based networks. With the rise of connected vehicles, C-V2X is emerging as one of the major standards for message transmission in automotive scenarios. The project aims to study the feasibility of C-V2X-based message transmission by building a prototype system, named <b>RampCast</b>, for transmitting traffic information from roadside message boards to vehicles. The RampCast framework would also implement geocasting-based algorithms to deliver messages to targeted vehicles. These algorithms focus on improving location-based message delivery using retransmission and prioritization strategies. The messages used for transmission are selected from the 511 web application built by INDOT, which contains the live traffic information for the state of Indiana which includes Travel Time information, Crash Alerts, Construction Alerts etc.</p><p dir="ltr">The major objectives of this project consist of building the RampCast prototype, a system implementing C-V2X networks using a Software Defined Radio(SDR). The RampCast system implements a Publisher-subscriber messaging architecture with the primary actors being a Road Side Unit(RSU) and a Vehicle Onboard Unit(OBU). A data store containing traffic messages sourced from the 511 API is set up to be the input to the RampCast system. An end-to-end message transmission pipeline is built that would implement message transmission algorithms on the RSU and OBU side. Finally, the performance of message transmission on the RampCast system is evaluated using a metrics-capturing module. The system was evaluated on a test track in Columbus, Indiana. The performance metrics of the system were captured and analyzed, and the system met the key performance indicators for Latency, Packet Delivery Rate, and Packet Inter-reception Rate. The results indicate the satisfactory performance of the C-V2X standard for message transmission in the RampCast traffic guidance scenarios.</p>

La bursite infectieuse aviaire (IBD) est une des causes majeures de pertes économiques pour l’industrie aviaire. La vaccination est le principal outil de contrôle de cette maladie et les oiseaux susceptibles doivent être vaccinés aussitôt que le niveau des anticorps maternels (MA) anti-IBDV est suffisamment bas. L’estimation du moment de vaccination est habituellement déterminée par la formule de Deventer qui utilise le titre initial de MA anti-IBDV et la demi-vie des anticorps pour prédire l’évolution du titre. Dans la présente étude, l’effet du gain de poids sur la vitesse de disparition des MA a été étudié dans le but de l’utiliser pour prédire la détermination du moment de la vaccination. L’analyse des taux d’anticorps neutralisants par ELISA a montré que les poussins avec une forte croissance avaient un taux de disparition plus rapide des MA que ceux à faible croissance. Une formule pour la prédiction du moment de vaccination contre le IBDV, basée sur le gain de poids et le niveau des MA a été développée et vérifiée. La prédiction du moment de vaccination avec cette formule a montré une haute corrélation avec les titres de MA mesurés par ELISA. Le virus de l’anémie infectieuse aviaire (CIAV) est une cause importante d’immunosuppression chez le poulet augmentant la pathogénicité des infections secondaires et en entraînant une réponse humorale suboptimale et une forte mortalité. D’autre part, l’infections sub-clinique du au CIAV provoque une immunosuppression qui facilite la coinfection par d’autre virus tel que le IBDV. Les effets de la coinfection à J1 avec une souche vaccinale de CIAV CAV-VAC® (Intervet) et à J14 avec une souche faiblement virulente de IBDV isolée au Québec, sur l’état de santé des poussins, sur la persistance virale et sur la réponse immunitaire ont été étudiés autant chez des poussins de 1 jour d’âge exempts d’agents pathogènes specifique (SPF) que ceux provenant d’élevages commerciaux. Les résultats ont montré que l’inoculation de la souche vaccinale du CIAV a entraîné une infection sub-clinique, une persistance virale dans la rate et le thymus, une altération de la thymopoièse et une réponse humorale temporaire chez les poussins SPF. Ces effets ont aussi été mis en évidence chez des poussins d’élevage commerciaux malgré des taux élevés de MA. Lors de l’infection avec la souche de IBDV chez des poussins déjà vaccinés contre le CIAV, la persistance du CIAV dans les organes lymphoïdes a été aggravée par une présence de réponses humorales temporaires contre les deux virus et une altération des populations lymphocytaires dans les organes lymphoïdes. Par contre, la présence des MA contre le CIAV a limité temporairement ces effets. Ces travaux ont mis en évidence des désordres immunitaires cellulaires et humoraux et une persistance virale chez des poussins vaccinés contre le CIAV et co-infectés avec le IBDV. / Infectious bursal disease (IBD) is one of the major causes of economic losses in the chicken industry. Vaccination is the main tool against the disease, and the susceptible birds should be vaccinated as soon as the maternal antibody (MA) becomes low enough to allow the vaccine to break through. Estimation of vaccination time is currently performed by Deventer formula which uses initial anti-IBDV titer and antibody half-life to predict the titer. Considering the increased growth rate of chicken in the last decades and the wide variations of MA, we have examined the effects of chick’s weight gain on MA decline and the use of weight in predicting IBD vaccination time. The virus neutralization test and ELISA results demonstrated that fast-growing birds had a faster rate of antibody decline whereas slow-growing birds demonstrated a slower rate. Based on the effect of weight-gain on maternal antibody decline, a new formula for predicting IBD vaccination time was introduced and tested. The predicted IBD vaccination time made by this weight formula showed higher correlation with the measured ELISA titers in the experiment. Chicken infectious anemia virus (CIAV) is another cause of immunosuppression in chicken which is characterized by increased pathogenicity of secondary infectious agents, sub-optimal antibody responses and mortality. CIAV subclinical infections can result in immunosuppression and enhancement of pathogenicity of co-infecting agents such as infectious bursal disease virus (IBDV). Effects of pathogenic CIAV and IBDV coinfection on chick’s health and immune responses are investigated in different studies. In this study, newly hatched specific pathogen free (SPF) and commercial chicks were vaccinated with CAV-VAC® (Intervet) vaccine and /or inoculated with a low-virulent Québec isolate of IBDV at 14 days post CIAV vaccination. Inoculation of the CIAV vaccinal strain at hatch resulted in subclinical infection associated with viral persistency in spleen and thymus, alteration of thymopoiesis and transient humoral response in SPF chicks. Subclinical infection, viral persistency and lack of antibody responses were also shown in CIAV inoculated commercial chicks with high MA. Infection of the low-virulent IBDV in the CIAV vaccinated SPF chicks lead to extended viral persistence of CIAV in lymphoid organs, transient immune responses to both CIAV and IBDV, and alteration of lymphocytes subpopulation in the lymphoid organs. In the coinfected commercial chicks, presence the CIAV in the lymphoid organs was controlled by MA in the first 1-2 weeks after hatch. Thereafter, the immune disorders, viral persistence and lack of humoral responses almost similar to the coinfected SPF chicks were recorded.