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Self-assembled octapeptide gels for cartilage repairMujeeb, Ayeesha January 2013 (has links)
Molecular self-assembly provides a simple and efficient route of constructing well-defined nanostructures which may serve as extra cellular matrix (ECM) mimics. This work focuses on two specific octapeptides: FEFEFKFK and FEFKFEFK (F: phenylalanine, E: glutamic acid, K: lysine) with alternating charge distribution. The peptides were shown to self-assemble in solution and form β-sheet rich nanofibres which, above a critical gelation concentration (CGC), entangle to form self-supporting hydrogels. The fibre morphology of the hydrogels was analysed using TEM and Cryo-SEM illustrating the dense fibrillar network of nanometer size fibres. Oscillatory rheology results showed that the hydrogels possesses viscoelastic properties. By varying peptide concentration and type hydrogel stiffness, viscosity, water content, fibre density and other mechanical properties were tailored to control cell interactions and subsequent tissue growth. Bovine chondrocytes were used to assess the biocompatibility of these novel scaffolds over 21 days under 2D and 3D cell culture conditions, particularly looking into cell morphology, proliferation and matrix deposition. 2D culture resulted in cell viability and collagen type I deposition. In 3D culture, the mechanically stable gel was shown to support viability, retention of cell morphology and collagen type II deposition. Subsequently, the scaffold may serve as a template for cartilage repair. In addition, this research also focused on developing novel injectable scaffold design with in situ gelation properties to encapsulate chondrocytes for cell culture applications.
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Controlled release of active compounds from a magnetic nanoparticle-vesicle aggregate nanomaterialBooth, Andrew January 2015 (has links)
Non-invasive and controlled release of bioactive compounds is an important goal in the development of drug delivery systems and novel biomaterials for tissue engineering. This project aims to exert spatio-temporal control over the release of bioactive compounds from phospholipid vesicle carriers by crosslinking them with superparamagnetic iron oxide nanoparticles to form a magnetic release nanostructure. The magnetic properties of the nanoparticles allow release to be triggered by an alternating magnetic field (AMF), which induces localised heating and “melts” the vesicle membranes. The aggregates can also be manipulated in space by a static magnetic field to create patterned materials. Incorporation of these aggregates into hydrogels has created novel responsive biomaterials. Controlled release of ascorbic acid-2-phosphate has been used to induce collagen production by chondrocytes, demonstrating an AMF triggered cellular response in vitro. The existing system has been redesigned after detailed characterisation and assessment of the performance of each component. Magnetic release has been extensively assessed using fluorescence techniques to quantify release, and optimised through the development of new silica-derived nanoparticle coatings and aggregate formulations informed by quantitative characterisation of nanoparticle functionalisation. The replacement of calcium alginate hydrogels as a 3D cell culture matrix with hyaluronic acid- based hydrogels was found to eliminate gel-induced leakage of vesicle contents and also improves the compatibility of the system with a greater range of cell types. Recently the effective encapsulation and AMF-triggered release of proteins including enzymes has been demonstrated and released enzymes have been demonstrated to retain their activity. Released trypsin was shown to retain proteolytic activity while hyaluronidase released into hyaluronan-derived hydrogels has been demonstrated to influence the rheological properties of the gel. A galactose-terminated lipid has been synthesised that enables specific targeting of the asialoglycoprotein (ASGPR) cell surface receptor receptor found in human hepatocytes, demonstrating the potential for customisation of the MNPV system to particular requirements.
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Estudo da citotoxicidade em celulas animais induzida pela ação da lectina de sementes de Talisia esculenta / Cytotoxicity in animals cells induced by lectin from Talisia esculenta seedsVentura, Claudio Angelo 18 August 2006 (has links)
Orientadores: Maria Ligia Rodrigues Macedo, Tomomasa Yano / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-08T00:07:13Z (GMT). No. of bitstreams: 1
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Previous issue date: 2006 / Resumo: Lectinas constituem uma classe de proteínas ou glicoproteínas que são capazes de ligar-se, reversivelmente e seletivamente, a carboidratos sem causar transformação química. Estudos têm mostrado que a ligação de lectinas a carboidratos de superficie celular de células normais e malignas leva a vários efeitos biológicos tais como proliferação celular e apoptose. Neste trabalho, nós investigamos os efeitos citotóxicos induzidos por TEL (uma lectina, isolada de sementes de Talisia esculenta, a qual preferencialmente liga-se a resíduos de manose) sobre linhagens de células tumorais e não-tumorais. Células Vero (rim de macaco verde afiicano) e Rela (carcinoma cervical humano) tratadas com TEL exibiram perda da integridade da membrana plasmática, retração celular, condensação da cromatina, fragmentação nuclear e formação de corpos apoptóticos. Além disso, células Vero e Rela tratadas com TEL também revelaram uma drástica desorganização do citoesqueleto de actina. A Fragmentação intemuc1eossomal do DNA foi detectada pelo método de TUNEL e eletroforese em gel de agarose. Os efeitos citotóxicos induzidos por TEL foram progressivos e mostraram que as alterações morfológicas e os danos ao DNA ocorreram após a perda da integridade da membrana. Adicionalmente, TEL inibiu significantemente a proliferação das células Rela de maneira dependente da concentração. Nós também mostramos através de microscopia de tluorescência que TEL é intemalizada nas células Vero dentro de pequenas vesículas que depois se acumulam da região perinuclear; nas células Rela, a intemalização não foi observada. Foi estabelecido que a atividade das caspases-3 e -9 aumentaram nas células Vero e Rela de uma maneira dependente do tempo. Em contraste com as células Vero, a atividade da caspase-3 precedeu a ativação da caspase9 nas células Rela. Os efeitos citotóxicos e a intemalização de TEL foram bloqueados pela mano se, sugerindo que a ligação de TEL ao carboidrato específico na superficie celular é um pré-requisito para esses processos. Os resultados mostraram que a morte celular induzida por TEL nas células Vero e Rela seguiu uma série de eventos que culminou em um modo apoptótico de morte celular. Finalmente, uma vez que as células Rela foram altamente sensíveis a citotoxicidade induzida pela lectina, TEL merece futuras investigações porque suas propriedades podem ser uma ferramenta útil na terapia contra câncer cervical humano / Abstract: Lectins constitute a class of proteins or glycoproteins which are capable of binding carbohydrates selectively and reversibly without causing their chemical transformation. Studies have shown that lectin binding to cell surface carbohydrates of normal and malignants cells triggers various biological effects such as cellular proliferation and apoptosis. In this work, we investigate the cytotoxic effects induced by TEL (a lectin, isolated from Talisia esculenta seeds, which binds preferentially to mannose residues), on tumoral and non-tumoral cell lines. TEL- treated Vero (African green monkey kidney) and Hela (human cervical carcinoma) cells exhibited loss of plasma membrane integrity, cellular retraction, chromatin condensation, nuclear fragmentation and formation of apoptotic bodies. Furthermore, TEL-treated Vero and Hela cells also revealed a drastic disorganization of the actin cytoeskeleton. Internucleossomal DNA fragmentation was detected by TUNEL method. and agarose gel electrophoresis. TEL-induced cytotoxic effects were progressive and showed that the morphological alterations and DNA damage occurred after the loss of membrane integrity. Additionally, TEL significantly inhibited the proliferation of Hela cells in a concentration- dependent manner. We also show through of fluorescence microspy that TEL is internalized in Vero cells into small visicles that further coalesce in the perinuclear region; in Hela cells, TEL internalization was not observed. It was established that caspase-3 and -9 activity increased in Vero and Hela cells after TEL treatment in a time- dependent manner. In contrast to Vero cells, caspase- 3 activity preceded the activation of caspase-9 in Hela cells. The cytotoxic effects and the internalization of TEL was blocked by manose, suggesting that the binding of TELto specific carbohydrate on the cell-surface is a prerequisite for these processes. The results showed that TEL- induced cell death in Vero and HeLa cells followed by down stream events leading to apoptotic mode of cell death. Finally, since Hela cells were highly sensitive to lectin-induced cytotoxicity, TEL merits further investigation due to its properties can be a useful tool in therapy against human cervical cancer / Doutorado / Bioquimica / Doutor em Biologia Funcional e Molecular
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Estruturação tridimensional de scaffolds de policaprolactona via manufatura aditiva / Additive manufacturing of PCL 3D scaffoldsSenedese, Ana Lívia Chemeli, 1984- 07 August 2011 (has links)
Orientadores: Rubens Maciel Filho, Jorge Vicente Lopes da Silva / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química / Made available in DSpace on 2018-08-18T15:30:30Z (GMT). No. of bitstreams: 1
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Previous issue date: 2011 / Resumo: Em decorrência da grande demanda de transplantes de órgãos e tecidos no Brasil, ha estímulos para criação de terapias alternativas como o desenvolvimento de substitutos biológicos temporários, isto e, scaffolds, através da Bioengenharia e Engenharia Tecidual, descartando a necessidade de doadores. Neste trabalho, foi utilizado o polímero policaprolactona (PCL) para estruturar scaffolds 3D por meio da plataforma experimental de manufatura aditiva Fab@CTI, a qual apresenta um cabeçote de extrusão intercambiável construído para entrada de material em forma de filamento. Uma nova proposta de orientação de raster foi criada para o design dos scaffolds. Foram estruturados scaffolds com raster regular e randômico com poros de 0.25, 0.5 e 1 mm. Analises de Difração de raios-x (DRX), Calorimetria exploratória diferencial (DSC) e Espectroscopia de absorção no infravermelho com transformada em Fourier (FTIR) foram realizadas para verificar possíveis mudanças nas propriedades térmicas do material durante o processamento. Microscopia eletrônica de varredura (MEV) foi feita para checar a morfologia dos scaffolds e medir o diâmetro dos filamentos extrudados na Fab@CTI alem do espaçamento entre eles. Também foram realizadas analises de citotoxicidade e viabilidade celular com células tronco mesenquimais de tecido adiposo. Ainda, utilizando o software modeFRONTIER, foi feita uma simulação de valores de modulo de compressão do PCL possíveis de serem obtidos. Os resultados de DRX e FTIR não mostraram degradação do PCL e o DSC revelou algumas alterações no ponto de fusão e diminuição da cristalinidade. Os scaffolds não se mostraram tóxicos e os modelos com poros de 0.25 mm e raster regular e randômico foram os que apresentaram viabilidade celular / Abstract: Due to the demand for organs and tissues transplants in Brazil, is indeed the creation of new therapies as the development of temporary biological substitutes, ie, scaffolds, through Bioengineering and Tissue Engineering, discarding the require for donors. In this work, we used the polymer polycaprolactone (PCL) to structure 3D scaffolds by the additive manufacturing experimental platform, Fab@CTI, which presents an interchangeable extrusion head build to filaments materials input. A new proposal for random raster was presented to design the scaffolds. Were structured scaffolds with regular and random raster and pores of 0.25, 0.5 and 1 mm. Analysis of X-Ray Diffraction (XRD), Differential Scanning Calorimetry (DSC) and Fourier Transform Spectroscopy (FTIR) were conducted to check for possible changes in thermal properties of the material during the processing. Scanning Electron Microscopy (SEM) was done to check the morphology of scaffolds and measure the diameter of filaments extruded at Fab@CTI beyond the distance between them. Analyses of cytotoxicity and cell viability with mesenchymal stem cells from adipose tissue were also performed. In addition, using the software modeFRONTIER, a simulation of compressive modulus of PCL was done with values possible to obtain. The result of the XRD and FTIR showed no degradation of PCL and DSC revealed some changes in melting point and decrease of crystallinity. The scaffolds were not toxic and models of 0.25 mm pores and both regular and random raster showed cell viability / Mestrado / Desenvolvimento de Processos Químicos / Mestre em Engenharia Química
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Melatonina 'in vitro' não exerce ações diretas em linhagens de células de hepatoma (HepG2) e insulinoma (MIN6) que expliquem sua capacidade de melhorar a homeostasia glicêmica / Melatonin 'in vitro' does not show direct effect on hepatoma (HepG2) and insulinoma (MIN6) cell lines that explain the improvement of glucose homeostasisBarbosa, Ana Paula de Lima, 1981- 26 August 2018 (has links)
Orientador: Gabriel Forato Anhê / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-26T19:10:52Z (GMT). No. of bitstreams: 1
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Previous issue date: 2014 / Resumo: O Diabetes Mellitus tipo II (DMT2) é uma doença caracterizada pela diminuição da sensibilidade à insulina e consequente prejuízo na homeostasia glicêmica. Estudos recentes mostraram que a melatonina, um hormônio produzido pela glândula pineal, melhora a tolerância à glicose e a resistência à insulina, diminui a expressão de proteínas ligadas à gliconeogênese hepática (G6Pase e PEPCK) e reduz a apoptose de células beta pancreáticas in vivo. Entretanto, ainda não estava claro se a melatonina conseguia, de fato, reverter in situ as ações lipotóxicas dos ácidos graxos saturados em células beta pancreáticas e na musculatura esquelética, e se podia diminuir a expressão dos genes reguladores da gliconeogênese hepática. Para responder a esse questionamento, utilizamos culturas celulares de insulinoma (MIN6), de músculo esquelético (miotubos de L6) e de hepatoma (HepG2). Os resultados mostraram que a melatonina reverte a resistência à insulina gerada por palmitato em miotubos de L6, mas somente em passagens baixas, não diminui a apoptose em MIN6 e não altera a expressão do G6pc, o gene que codifica a G6Pase, em HepG2 / Abstract: Type II Diabetes Mellitus (T2DM) is characterized by decrease of insulin sensitivity, resulting impairment glucose homeostasis. Recent studies have shown that melatonin, a hormone produced by the pineal gland, improves glucose tolerance and insulin resistance, reduce the expression of hepatic gluconeogenesis proteins (G6Pase and PEPCK) and diminish pancreatic beta cell apoptosis in vivo. However, it was unclear if melatonin could reverse the lipotoxic actions of saturated fatty acid in pancreatic beta cells and skeletal muscle, and if melatonin could decrease the expression of regulatory genes of hepatic gluconeogenesis in situ. To answer this question, we used cell cultures of insulinoma (MIN6), skeletal muscle (L6 myotubes) and hepatoma (HepG2). The results showed that melatonin reverses palmitate insulin resistance in L6 myotubes, but only at low passages, melatonin does not decrease apoptosis in MIN6 and does not alter the expression of G6pc, the encoding gene of G6Pase, in culture cell of HepG2 / Doutorado / Farmacologia / Doutora em Farmacologia
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Estudo do significado biológico da multinucleação induzida por vincristina em células em cultura / The study of biological meaning of multinucleation induced by vincristine in cultured cellsElly Kayoko Nakagawa 23 October 2006 (has links)
O estudo de agentes que interferem no funcionamento das proteínas relacionadas com o ciclo celular é importante para a compreensão dos processos de transformação e de morte celular. Alterações de ploidia, embora presentes na maioria dos tumores humanos, não têm ainda seu papel conhecido no processo de oncogênese. A alteração do número cromossômico é conseqüência primária de erros que envolvem o fuso mitótico e o cinetócoro. Dessa maneira, drogas que agem sobre os microtúbulos são consideradas aneugênicas potenciais. O presente trabalho enfocou o estudo do mecanismo pelo qual drogas que atuam sobre microtúbulos levam ao aparecimento de células multinucleadas e o significado biológico destas. Os resultados mostraram que a vincristina induziu bloqueio em mitose das células BBnt e MDCK, com conseqüente entrada em interfase no estado multinucleado. As células multinucleadas não apresentaram sinais de morte celular por apoptose, entretanto, quando em prófase apresentaram vários centrossomos, que poderiam originar divisões celulares com fusos multipolares. Estes resultados indicam que essas linhagens celulares possuem pontos de checagem mitótico funcionais e células multinucleadas são viáveis e capazes de prosseguir no ciclo celular. A presença de mitoses com fusos multipolares é indício de que as células multinucleadas passam por divisões anormais, que progrediriam para apoptose resultando na eliminação desta população. / The study of agents that interfere in the functionality of proteins related to cell cycle is important for the understanding of the transformation and cell death processes. Although ploidy alterations are presented in the majority of human tumors, their role in oncogenic process is not understood yet. The alteration on chromosomal number is the primary consequence of errors involving the mitotic spindle and kinetocore. Thus, drugs acting on the microtubules are considered as potentially aneugenic agents. The present work aimed to study the mechanism of multinucleated cells induction by action of antimicrotubule drug and biological meaning of these cells. The results showed that vincristine induced mitotic arrest of both BBnt and MDCK cells, with consequent entrance into interphasic-multinucleated status. Multinucleated cells did not present features of cell death by apoptosis; they were still viable and able to go further in cell-cycle progression. The presence of many structures suggested microtubule enucleation, centrosomes-like were detected on treated cells and could be responsible for the multi-spindle assembling that leads the multinucleated cells to abnormal divisions. Later on, when the multinucleated cells accumulated more abnormalities they were eliminated from the cell population by apoptosis.
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Influência de diferentes meios de cultura em cultivos de Streptococcus pneumoniae sorotipo 1 para produção de polissacarídeo capsular. / Influence of different culture media on Streptococcus pneumoniae serotype 1 cultivations to produce capsular polysaccharide.Bruno Vitório Marthos 03 August 2012 (has links)
S. pneumoniae é um importante patógeno humano que afeta sobretudo crianças. Vacinas são a principal estratégia de combate e o polissacarídeo (PS) da cápsula é o antígeno. Dentre os sorotipos do patógeno, o tipo 1 é prevalente em crianças e o PS1 componente obrigatório das vacinas. Neste trabalho objetivou-se: estabelecer um método de dosagem do PS1, selecionar a melhor cepa produtora de PS1, avaliar 3 peptonas, investigar 4 componentes do meio de cultura em planejamento experimental (PE) em reator e propor um novo meio e estratégia de cultivo. A cepa ST595/01 foi selecionada e o Phytone (Phy) foi a peptona com maior produção de PS1, 298mg/L, medido por m-hidroxidifenil com sulfamato. O PE 24-1 com extrato de levedura (EL), Phy, Asn e Gln mostrou os maiores efeitos positivos do Phy para produção de biomassa (Cx) e PS1. EL foi positivo para Cx, Asn não apresentou efeitos e Gln menor efeito positivo para PS1. Assim, testou-se o novo meio com Phy 15g/L e EL 2g/L sob duas estratégias: a batelada alimentada superou a batelada simples em 2x para Cx e 2,5x para PS1. / S. pneumoniae is an important human pathogen that affects mainly children. Vaccines are the main strategy to fight the disease and capsular polysaccharide (PS) is the antigen. Among pneumococcal serotypes, type 1 is prevalent in children. The aims of this work were: establish a method to measure PS1, screen strains for the best PS1 producer, evaluate 3 peptones, investigate the effects of 4 medium components by a design of experiment (DoE) and propose a new medium/strategy for cultivation. The strain ST595/01 was selected. Phytone (Phy) was the peptone with the highest PS1 production (298mg/L, measured by m-hydroxydiphenyl + sulfamate method). DoE 24-1 was carried out to assess the effects of yeast extract (YE), Phy, Asn and Gln. Phy showed the major positive effects for biomass (Cx) and PS1. YE demonstrated effects for Cx. Gln showed minor effect on PS1 production and Asn did not present effects. A new medium based on 15g/L Phy and 2g/L YE was evaluated by 2 strategies: fed-batch showed Cx production 2-fold higher and PS1 2.5-fold higher than simple batch.
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Gene Transfer Into the Inner EarOestreicher, David 30 October 2019 (has links)
No description available.
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Development of PVDF micro and nanostructures for cell culture studies / Développement de PVDF micro et nanostructures pour des études de culture cellulaireLhoste, Kévin 30 November 2012 (has links)
L'ingénierie tissulaire vise à réparer les tissus endommagés et à récupérer les fonctions biologiques correspondantes. Afin de restaurer un tissu endommagé tel que le système nerveux, la conception et la fabrication de nouveaux types d’échafaudages tissulaires sont nécessaires. Dans ce travail, nous avons développé plusieurs techniques de microfabrication pour le polyfluorure de vinylidène (PVDF), un fluoropolymère thermoplastique, non réactif et piézoélectrique, qui peut être utilisé pour la culture cellulaire et l'ingénierie tissulaire. Nous avons tout d'abord étudié l'adhésion et la croissance cellulaire sur des substrats en PVDF avec des motifs micro et nanométriques en utilisant différentes techniques de fabrication telles que la micro-photolithographie, la lithographie douce, l’impression par microcontact, etc. L'influence de la micro-structuration sur les activités piézo-électriques du PVDF a été caractérisée par différentes méthodes d'analyses de surface (FTIR, XRD). Par la suite, nous avons effectué une étude systématique sur la fabrication de nanofibres de PVDF et leur compatibilité avec la culture cellulaire. Enfin, nous avons démontré la possibilité de doper ces nanofibres avec des nanoparticules magnétiques ce qui les rends excitables à distance par un champ magnétique / Tissue engineering aims at repairing damaged tissues and recovering the lost or degraded biological functions with artificial scaffolds. In order to meet the requirement for more complex functionality such as peripheral nerve reconstruction, new types of scaffold materials are needed. In this work, we developed several micro- and nanofabrication techniques to pattern polyvinylidene fluoride (PVDF), a highly non-reactive, piezoelectric, thermoplastic fluoropolymer, which can serve as new constituents for advanced tissue engineering. We first studied the feasibility of PVDF patterning using conventional photolithography, soft lithography and microcontact printing. The fabricated patterns were systematically characterized by surface analysis techniques (FTIR, XRD) and used for cell culture studies. Then, we developed a study on electrospinning of PVDF nanofibers. Our results showed that the fabricated PVDF nanofibers were compatible with cell-based assays. Finally, we doped electrospun PVDF nanofibers with magnetic nanoparticles, which should make them excitable with a remote magnetic field
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Method Development for Efficient Incorporation of Unnatural Amino AcidsHarris, Paul D. 04 1900 (has links)
The synthesis of proteins bearing unnatural amino acids has the potential to enhance and elucidate many processes in biochemistry and molecular biology. There are two primary methods for site specific unnatural amino acid incorporation, both of which use the cell’s native protein translating machinery: in vitro chemical acylation of suppressor tRNAs and the use of orthogonal amino acyl tRNA synthetases. Total chemical synthesis is theoretically possible, but current methods severely limit the maximum size of the product protein. In vivo orthogonal synthetase methods suffer from the high cost of the unnatural amino acid. In this thesis I sought to address this limitation by increasing cell density, first in shake flasks and then in a bioreactor in order to increase the yield of protein per amount of unnatural amino acid used. In a parallel project, I used the in vitro chemical acylation system to incorporate several unnatural amino acids, key among them the fluorophore BODIPYFL, with the aim of producing site specifically fluorescently labeled protein for single molecule FRET studies. I demonstrated successful incorporation of these amino acids into the trial protein GFP, although incorporation was not demonstrated in the final target, FEN1. This also served to confirm the effectiveness of a new procedure developed for chemical acylation.
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