Psicólogas / Autor no autoriza el acceso a texto completo de su documento. / El presente estudio se realizó en la ex Clínica Clandestina Santa Lucía, y tiene como objetivo indagar en el cómo es presentado este espacio por los habitantes del lugar. La metodología utilizada es de tipo cualitativa, donde la técnica de producción de datos fue la de “recorridos comentados”. Los resultados del estudio destacan la experiencia propia de los habitantes como lugar central a la hora de presentar el espacio, así como el uso del espacio en tanto recurso escénico y como interlocutor clave durante la situación interaccional

En este trabajo pretendemos analizar el personaje de Celestina y el mundo de la prostituciónclandestina en la Tragicomedia de Calisto y Melibea de Fernando de Rojas en su contextosociohistórico. La obra se escribió en el momento de un cambio fundamental en el mundo dela prostitución en la sociedad española medieval y el objetivo de nuestro trabajo es analizarcómo estos cambios se han reflejado en la obra estudiada, en particular en la vida y accionesde uno de sus personajes, Celestina. Nos interesa investigar cómo se presenta este personajeen relación a la prostitución, cómo los cambios sociohistóricos en el mundo de la prostituciónde la TCM han afectado a la vida y acciones de Celestina y qué tipos de prostituciónclandestina se retratan en la obra en relación con ella. Primero presentamos la situación contemporánea en el mundo de la prostitución en Españaen la Baja Edad Media en el momento en que se introdujeron los burdeles públicos y seprohibió la prostitución libre, los cambios en la estructura existente de la prostitución que estecambio supuso y los tipos de prostitución clandestina que cristalizaron tras este cambio. Estecontexto nos permite realizar el análisis del personaje de Celestina, concebir su perfil ehistoria, analizar las causas de los acontecimientos en su vida y comprender los motivos desus decisiones y acciones. Finalmente se presentan dos tipos de la prostitución clandestina ysu relación con Celestina. La elección de Celestina como sujeto literario analizado nospermite capturar de manera bastante detallada el cambio sociohistórico de la época. El punto de partida ideológico para la realización de nuestro análisis radica en el abordajesocial y psicológico que nos permite analizar el personaje de Celestina sin juzgar ni condenarsu vida y comportamiento, sino comprenderlos y explicarlos dentro de su dimensión social ypsicológica.

Made available in DSpace on 2016-04-28T19:59:07Z (GMT). No. of bitstreams: 1 Conceicao da Silva Zacheu Russo.pdf: 637030 bytes, checksum: 90e482bfc5b01f4d501b40db868b1c5d (MD5) Previous issue date: 2007-10-30 / This paper seeks to understand the method for building happiness in the short stories of Clarice Lispector. The goal is to reflect upon the question of happiness, asking whether happiness can be associated to a moment of lucidity, of self understanding, associated to an epiphany. To this investigation, the following hypothesis were considered: that happiness is written in the writing process and the paradox is one of the keys to this process; that the search for the construction of happiness reflects the union of joys and agonies of human beings, and that the happiness metaphor reveals the proceedings to reach the epiphany. The following short stories were selected to this study: Felicidade Clandestina (Clandestine Happiness) and Feliz Aniversário (Happy Birthday). The theoretical base that supports this analysis is focused on three pillars. Concerning happiness, the philosophical concepts of Aristotle and Epicurus were prioritized. As to the aesthetic effects projected by the discursive action, the references searched were Poe, Cortázar and Sant Anna. To discuss happiness and epiphany, the base used here is the studies of Olga de Sá. This paper explores in its three chapters the genesis of the happiness discourse, the structure of the claricean short story, the story analysis, the happiness dialectic, and the contradictions of beings and language that result in texts built without a happy end. Amongst other conclusions, it was found that in the process of building the happiness discourse some existential truths are revealed in fractions of seconds, and those existential truths question themselves continuously, emulating the contradictions of human beings in finding happiness with an epiphany / A presente dissertação busca apreender o método de construção da felicidade em contos de Clarice Lispector. Objetivando refletir sobre a problemática da felicidade, perguntamo-nos se a felicidade pode ser associada a um momento de lucidez, de entendimento do próprio eu , associando-se à epifania. Consideramos para esta investigação, as seguintes hipóteses: a felicidade está inscrita no processo de escritura, sendo o paradoxo uma das chaves desse processo; a busca da construção da felicidade reflete a união das alegrias e agonias do ser humano e a metáfora da felicidade revela os procedimentos para se chegar à epifania. Para o estudo, foram selecionados os contos: Felicidade Clandestina e Feliz Aniversário . A fundamentação teórica que sustenta a análise é alicerçada em três pilares. No que diz respeito à felicidade, priorizamos os conceitos filosóficos de Aristóteles e Epicuro. Com relação aos efeitos estéticos projetados pela ação discursiva, buscamos referências em Poe, Cortázar e Sant Anna. Para a discussão da felicidade e da epifania, fundamentamo-nos, primordialmente, nos estudos de Olga de Sá. O trabalho procura explorar, ao longo de três capítulos, a gênese do discurso da felicidade, a estrutura do conto clariceano, a análise dos contos, a dialética da felicidade e as contradições do ser e da linguagem que resultam na construção de textos sem happy end. Entre outras conclusões, apreende-se, no processo de construção do discurso da felicidade, a revelação, em frações de segundos, de verdades existenciais que se questionam continuamente, a mimetização das contradições do ser humano no encontro da felicidade com a epifania

O objetivo da pesquisa é discutir as redes de subcontratação e o trabalho informal no circuito das confecções em São Paulo. A partir de uma região periférica na zona leste da cidade tratou-se de averiguar as relações entre trabalho e o espaço urbano no qual essas confecções vêm se instalando. O processo de reestruturação produtiva da indústria de confecções durante a década de 1990 fez multiplicar as chamadas oficinas de fundo de quintal e o trabalho a domicílio nos bairros das ex-costureiras das fábricas pelas vias de redes de subcontratação e do trabalho informal. As ex-operárias mobilizam familiares e vizinhos no trabalho, estabelecendo redes sociais pelas quais circulam as encomendas de costura. Associado a esta dinâmica, esse circuito também vem mobilizando os fluxos da migração clandestina dos bolivianos que já podem ser encontrados nos locais mais distantes do extremo leste da cidade. O material da pesquisa é composto por trajetórias sociais de pessoas ligadas ao setor, recompostas com entrevistas gravadas e semi-diretivas, e acompanhadas por pesquisa de campo. / The aim of this study is to discuss the sub-hiring and informal labor networks that have been developing in the garment industry of Sao Paulo. Focusing on a suburban eastern area of the city, the research was carried out in order to analyze the labor relations and the urban space in which this informal market is being settled. The productive adjustment process that took place in the garment industry during the 1990s led to an increasing number of small garment workshops and in domicile work in the neighborhoods where dressmakers used to work for factories. This growth in the informal market took place through the sub-hiring and informal labor networks. The ex-dressmakers mobilize relatives and neighbors to work in this field, establishing the social networks that keep the garment orders active. Due to this dynamic process, this circuit has been mobilizing the flow of clandestine migration of Bolivian people, who can be found in the further eastern neighborhoods of the city. The research corpus is composed by social trajectories of people related to this sector, mainly through semi-directive recorded interviews and followed by field research.

O objetivo desta tese é demonstrar que a maior eficácia da prova produzida no exterior e a maior eficiência da cooperação jurídica internacional estão associadas ao padrão normativo universal dos direitos humanos, que possibilita a superação do principal entrave que é a diversidade entre os sistemas jurídicos nacionais. A Declaração Universal de 1948 constitui o grande referencial histórico na construção do padrão normativo universal dos direitos humanos. O movimento de difusão e consolidação do padrão normativo universal dos direitos humanos deu-se através dos processos de internacionalização e constitucionalização, traduzido num esforço de harmonização, em que se buscou estabelecer uma relação de equivalência e semelhança entre os sistemas, respeitada a diversidade que particulariza cada um destes. A aceitação do modelo normativo universal dos direitos humanos repercute no processo penal, seja na atividade probatória como no instrumento processual da assistência jurídica internacional. Na atividade probatória, o padrão dos direitos humanos manifesta-se pelo modelo de processo justo, que representa o marco comum entre os sistemas jurídicos nacionais que o incorporaram, inclusive o brasileiro, sendo suficiente a sua observância para que a eficácia da prova seja idoneamente alcançada. Na cooperação jurídica internacional, o padrão dos direitos humanos qualifica-se como o pressuposto para a maior confiança entre os Estados que o adotam, possibilitando a revisão do modelo tradicional e a adoção de formas mais ágeis e diretas de assistência. Nesse contexto, o ordenamento jurídico brasileiro impõe um novo tratamento normativo do instituto da cooperação jurídica internacional para a produção de prova, levando-se em consideração a tendência internacionalista assumida a partir da Constituição Federal de 1988, seja por reconhecer a ordem internacional como um dos fundamentos do Estado Democrático brasileiro, seja por eleger os direitos humanos e a cooperação entre os povos para o progresso da humanidade como princípios a reger as suas relações internacionais. / The aim of the thesis is demonstrate that the effectiveness of the evidence obtained abroad and the efficiency of the international legal assistance are related to the universal legal standard of human rights, which allows a response for the diversity between national legal systems. The Universal Charter of 1948 is the main marc in the process of building the universal legal standard of human rights. The movement of diffusion and consolidation of the universal legal standard of human rights is related to the process of internationalization, either in the constitutional level, which work was an effort of harmonization, in a way of introducing the same values, not the same rules, in the national legal systems, considering the diversity of each one. The acceptance of the universal legal model of human rights affects the procedural criminal law, even the evidence and the international legal assistance. Regarding the evidence the universal legal standard of human rights means the fair trial model, a connection between the national legal systems that introduced this model, either the Brazilian legal order, which is enough to guarantee the effectiveness of the evidence. Regarding the international legal assistance the universal legal standard of human rights means the basis for more confidence between the states that adopt this one, and which allows a revision of the traditional model and the use of different ways of cooperation, faster and direct. In this sense, the Brazilian legal system obeys a new legal model of international assistance to obtain evidence abroad, considering the international tendency affirmed by Federal Constitution of 1988, which recognizes the international order in the basis of the Brazilian Democratic State, and the human rights and the cooperation for the progress of humanity as principles to guide its international relationship.

Made available in DSpace on 2016-04-28T19:59:00Z (GMT). No. of bitstreams: 1 LCL - Elcio Luis Roefero.pdf: 379979 bytes, checksum: 3193a04f7f56e8bfdaba6b35163a75e3 (MD5) Previous issue date: 2006-10-17 / Secretaria do Estado e Educação / In this Master s thesis we seek to read the concept of desire in Clarice Lispector, starting from the study of O primeiro beijo [The first Kiss], Tentação [Temptation], Felicidade clandestina [Clandestine Happiness], Restos do carnaval [The remains of Carnival] e Os desastres de Sofia [Sofia s disasters], which comprise the book of short stories entitled Felicidade clandestina, published in 1971. Each short story chosen for this work points to a different movement of desire and of learning towards the subject construction. Thus, from the desiring state, the character in Clarice Lispector dives into a realm of self-discovery, wandering from joy to frustration essential in the constant search of themselves. While analyzing this corpus, our main theoretical framework was the convergence of Literature and Psychoanalysis, since the line of research that we follow allows for the study of Lispector s characters always torn between the spoken and the non-spoken. In the introduction, we went through several of the readings carried out about desire and its constitution, finding echo in Freud theories as well as in those of his followers besides taking a glance at the philosophical thought as well. In the first chapter, we weaved our theoretical path between Literature and Psychoanalysis. The following chapters were dedicated to the analyses of the chosen short stories, culminating with the study of Os desastres de Sofia a story that anticipates recurring themes found in Lispector s short stories, especially in terms of childhood constitution as a risky space. When we selected to investigate desire in Lispector s fiction, as well as the unfolding elements and frustrations, we noticed that there was a typical organization of the different forms, by which the characters relations, identities and alterities were interwoven. At the same time, one can see a fertile terrain in which the Other s unknown and flourish in confrontation with the self s unknown / Propomos, nesta dissertação de mestrado, uma leitura do desejo na obra de Clarice Lispector, partindo do estudo de O primeiro beijo , Tentação , Felicidade clandestina , Restos do carnaval e Os desastres de Sofia , que compõem o volume de contos Felicidade clandestina, publicado em 1971. Cada conto escolhido aponta para um diferente movimento do desejo e sua aprendizagem na construção do sujeito. Dessa forma, a partir de um estado desejante, a personagem clariciana mergulha num processo de auto-descoberta, que transita entre o gozo e a frustração, essenciais na busca constante que operam em si mesmas. Na análise desse corpus, usamos como principal referencial teórico a confluência Literatura/Psicanálise, uma vez que a linha de pesquisa privilegia o estudo das personagens de Lispector, sempre inseridas num limiar entre o dizer e o não-dizer. Na introdução desse trabalho, percorremos várias leituras acerca do desejo e sua possível constituição, encontrando ecos nas teorias de Freud e seus seguidores, além de uma breve inserção no pensamento filosófico. No primeiro capítulo, tecemos um percurso teórico das relações entre Literatura e Psicanálise. Nos capítulos seguintes, detemo-nos nas análises dos contos escolhidos, culminando no estudo de Os desastres de Sofia , obra que antecipa recorrentes temas da contística clariciana, sobretudo a constituição da infância enquanto espaço de risco. Ao elegermos a investigação do desejo na ficção de Lispector, bem como os seus desdobramentos e frustrações, percebemos uma organização das diferentes formas de configuração das relações de personagem, identidade e alteridade. Ao mesmo tempo, vislumbramos um terreno fértil onde floresce o desconhecido do Outro em confronto com o desconhecido de si

O objetivo da pesquisa é discutir as redes de subcontratação e o trabalho informal no circuito das confecções em São Paulo. A partir de uma região periférica na zona leste da cidade tratou-se de averiguar as relações entre trabalho e o espaço urbano no qual essas confecções vêm se instalando. O processo de reestruturação produtiva da indústria de confecções durante a década de 1990 fez multiplicar as chamadas oficinas de fundo de quintal e o trabalho a domicílio nos bairros das ex-costureiras das fábricas pelas vias de redes de subcontratação e do trabalho informal. As ex-operárias mobilizam familiares e vizinhos no trabalho, estabelecendo redes sociais pelas quais circulam as encomendas de costura. Associado a esta dinâmica, esse circuito também vem mobilizando os fluxos da migração clandestina dos bolivianos que já podem ser encontrados nos locais mais distantes do extremo leste da cidade. O material da pesquisa é composto por trajetórias sociais de pessoas ligadas ao setor, recompostas com entrevistas gravadas e semi-diretivas, e acompanhadas por pesquisa de campo. / The aim of this study is to discuss the sub-hiring and informal labor networks that have been developing in the garment industry of Sao Paulo. Focusing on a suburban eastern area of the city, the research was carried out in order to analyze the labor relations and the urban space in which this informal market is being settled. The productive adjustment process that took place in the garment industry during the 1990s led to an increasing number of small garment workshops and in domicile work in the neighborhoods where dressmakers used to work for factories. This growth in the informal market took place through the sub-hiring and informal labor networks. The ex-dressmakers mobilize relatives and neighbors to work in this field, establishing the social networks that keep the garment orders active. Due to this dynamic process, this circuit has been mobilizing the flow of clandestine migration of Bolivian people, who can be found in the further eastern neighborhoods of the city. The research corpus is composed by social trajectories of people related to this sector, mainly through semi-directive recorded interviews and followed by field research.

O objetivo desta tese é demonstrar que a maior eficácia da prova produzida no exterior e a maior eficiência da cooperação jurídica internacional estão associadas ao padrão normativo universal dos direitos humanos, que possibilita a superação do principal entrave que é a diversidade entre os sistemas jurídicos nacionais. A Declaração Universal de 1948 constitui o grande referencial histórico na construção do padrão normativo universal dos direitos humanos. O movimento de difusão e consolidação do padrão normativo universal dos direitos humanos deu-se através dos processos de internacionalização e constitucionalização, traduzido num esforço de harmonização, em que se buscou estabelecer uma relação de equivalência e semelhança entre os sistemas, respeitada a diversidade que particulariza cada um destes. A aceitação do modelo normativo universal dos direitos humanos repercute no processo penal, seja na atividade probatória como no instrumento processual da assistência jurídica internacional. Na atividade probatória, o padrão dos direitos humanos manifesta-se pelo modelo de processo justo, que representa o marco comum entre os sistemas jurídicos nacionais que o incorporaram, inclusive o brasileiro, sendo suficiente a sua observância para que a eficácia da prova seja idoneamente alcançada. Na cooperação jurídica internacional, o padrão dos direitos humanos qualifica-se como o pressuposto para a maior confiança entre os Estados que o adotam, possibilitando a revisão do modelo tradicional e a adoção de formas mais ágeis e diretas de assistência. Nesse contexto, o ordenamento jurídico brasileiro impõe um novo tratamento normativo do instituto da cooperação jurídica internacional para a produção de prova, levando-se em consideração a tendência internacionalista assumida a partir da Constituição Federal de 1988, seja por reconhecer a ordem internacional como um dos fundamentos do Estado Democrático brasileiro, seja por eleger os direitos humanos e a cooperação entre os povos para o progresso da humanidade como princípios a reger as suas relações internacionais. / The aim of the thesis is demonstrate that the effectiveness of the evidence obtained abroad and the efficiency of the international legal assistance are related to the universal legal standard of human rights, which allows a response for the diversity between national legal systems. The Universal Charter of 1948 is the main marc in the process of building the universal legal standard of human rights. The movement of diffusion and consolidation of the universal legal standard of human rights is related to the process of internationalization, either in the constitutional level, which work was an effort of harmonization, in a way of introducing the same values, not the same rules, in the national legal systems, considering the diversity of each one. The acceptance of the universal legal model of human rights affects the procedural criminal law, even the evidence and the international legal assistance. Regarding the evidence the universal legal standard of human rights means the fair trial model, a connection between the national legal systems that introduced this model, either the Brazilian legal order, which is enough to guarantee the effectiveness of the evidence. Regarding the international legal assistance the universal legal standard of human rights means the basis for more confidence between the states that adopt this one, and which allows a revision of the traditional model and the use of different ways of cooperation, faster and direct. In this sense, the Brazilian legal system obeys a new legal model of international assistance to obtain evidence abroad, considering the international tendency affirmed by Federal Constitution of 1988, which recognizes the international order in the basis of the Brazilian Democratic State, and the human rights and the cooperation for the progress of humanity as principles to guide its international relationship.

Made available in DSpace on 2015-10-19T11:49:46Z (GMT). No. of bitstreams: 1 anacastro2002.pdf: 1522039 bytes, checksum: 6fff49686a70f4c5a0af6aa20a50684e (MD5) Previous issue date: 2002-04-11 / Theorical proposal that pretends to analyze the process of institutionalization of collective memories, starting from the Torture Never More Group, in order to understand the degree of institutional negotiations face the reports of the country's political events in recent history, in its fight to impede the exclusion of the official memory. To estimate the mechanisms engendered in the formation of the Clandestine Memories, found by the concept elaborated by Michel Pollak (1989), inserted in the resistance of the groups caught by extreme situations and its struggle for the right to information as survival condition. To perceive, in the crossing from the collective to institutional, and probable museum incorporations, how the collective memories are organized and articulated to avoid meaning, information and communication losses, from the informational stock of relevant social and politic expression, so to minimize the breaching of the grupal identification burden and the fragmentation of its living's contents / Analisar o processo de institucionalização das memórias coletivas a partir do Grupo Tortura Nunca Mais, a fim de compreender as etapas de negociações institucionais face aos relatos dos acontecimentos políticos da história recente do país, em sua luta para impedir a exclusão da memória oficial. Avaliar os mecanismos engendrados na formação das memórias clandestinas, com base no conceito elaborado por Michel Pollak (1989), inserido em mecanismo de resistência de grupos atingidos por situações-limite em sua luta pelo direito à informação como condição de sobrevivência. Perceber na passagem de coletiva a institucional e, por conseqüência das incorporações museais, possíveis perdas de significado, informação e comunicação, ao sofrer alterações de conteúdo e romper a carga identitária grupal, fragmentando seu continente de vivência

"Το τσάι του βουνού" (στο οποίο ανήκουν πολλά είδη του γένους Sideritis) καταναλώνεται παραδοσιακά στην Ελλάδα ως ηρεμιστικό αλλά και ενάντια του κρυολογήματος και αλλεργιών. Η παρούσα μελέτη διερευνά την πιθανή νευροπροστατευτική δράση του ανωτέρω φυτού και εστιάζεται: α) στην επίδραση του αφεψήματος του φυτού Sideritis clandestina subsp. clandestina σε συμπεριφερικές παραμέτρους ενηλίκων μυών (άγχος/φόβος, μνήμη/μάθηση), β) στην επίδραση του αφεψήματος του ανωτέρω φυτού σε βιοχημικές παραμέτρους και πιο συγκεκριμένα στη συγκέντρωση της ανηγμένης γλουταθειόνης, στην υπεροξείδωση λιπιδίων και στην ενεργότητα δυο ισομορφών του ενζύμου της ακετυλοχολινεστεράσης (AChE) και γ) στην in vitro πιθανή αντιχολινεστερασική και αντιαμυλοειδική δράση του υδατικού εκχυλίσματος του φυτού. Το φυτικό αφέψημα σε συγκέντρωση 4% w/v χορηγoόταν καθημερινά σε αρσενικούς Balb-c μύες για περίοδο 40 ημερών. Για την εκτίμηση του άγχους/φόβου χρησιμοποιήθηκε α) η δοκιμασία του Υπερυψωμένου Λαβυρίνθου (χρόνος παραμονής των μυών στους ανοιχτούς βραχίονες ως προς το συνολικό χρόνο παραμονής στους ανοιχτούς και κλειστούς βραχίονες της συσκευής) και β) η δοκιμασία του Θιγμοτακτισμού (τάση παραμονής των μυών πλησίον των τοιχωμάτων του ειδικού κλωβού). Η μνήμη-μάθηση μελετήθηκε με τη δοκιμασία της Παθητικής Αποφυγής η οποία βασίζεται στην παρατήρηση ότι τα πειραματόζωα θα θυμούνται ότι μια συγκεκριμένη αντίδρασή τους θα έχει αρνητικές συνέπειες (εφαρμογή επώδυνου ηλεκτρικού ερεθίσματος στα άκρα). Οι οξειδωτικές/αντιοξειδωτικές ιδιότητες του φυτικού αφεψήματος προσδιορίστηκαν με εκτίμηση α) της συγκέντρωσης του αντιοξειδωτικού δείκτη της ανηγμένης γλουταθειόνης, η οποία στηρίζεται στο σχηματισμό ενός φθορίζοντος συμπλόκου μετά την αντίδραση της ο-φθαλαλδεϋδης με τη γλουταθειόνη και υστιδύλ-ενώσεις και β) τα επίπεδα λιπιδικής υπεροξείδωσης μέσω προσδιορισμού των επιπέδων του οξειδωτικού δείκτη της μηλονικής διαλδεΰδης που στηρίζεται στο σχηματισμό του φθορίζοντος συμπλόκου που δημιουργείται όταν η μηλονική αλδεΰδη αντιδρά με το θειοβαρβιτουρικό οξύ. Η ενεργότητα του ενζύμου της ακετυλοχολινεστεράσης (AChE) τόσο ex vivo όσο και in vitro προσδιορίστηκε με τη χρήση της χρωματομετρικής μεθόδου του Ellman, όπου ως υπόστρωμα του ενζύμου χρησιμοποιήθηκε η ιωδιούχος ακετυλοθειοχολίνη. Κατά τον προσδιορισμό της ενεργότητας του ενζύμου in vitro ως εσωτερικό πρότυπο χρησιμοποιήθηκε η γαλανταμίνη, ένας ισχυρός αναστολέας του ενζύμου. Η πιθανή αντιαμυλοειδκή δράση του φυτικού εκχυλίσματος μελετήθηκε με τη μέθοδο της θειοφλαβίνης-Τ. Τα αποτελέσματα της δοκιμασίας του Υπερυψωμένου Λαβύρινθου έδειξαν ότι ο χρόνος παραμονής στους ανοιχτούς βραχίονες ως προς το συνολικό χρόνο παραμονής στους ανοιχτούς και κλειστούς βραχίονες της συσκευής είναι στατιστικώς σημαντικά αυξημένος για την ομάδα των ζώων που κατανάλωσαν το φυτικό αφέψημα σε σύγκριση με τους μάρτυρες. Από τη δοκιμασία του Θιγμοτακτισμού για την πειραματική ομάδα φάνηκε ότι ο χρόνος θιγμοτακτισμού μειώνεται και ο αριθμός των εισόδων στην κεντρική περιοχή του ανοιχτού πεδίου αυξάνεται για κάθε 5 λεπτά παραμονής (εκ του συνολικού διαστήματος των 30 λεπτών) στη συσκευή εν συγκρίσει με την ομάδα των μαρτύρων. Τέλος, κατά τη δοκιμασία της Παθητικής Αποφυγής ο αρχικός και τελικός λανθάνων χρόνος (IL, STL αντίστοιχα) φάνηκε να μη διαφέρουν μεταξύ των δυο ομάδων πειραματοζώων. Η πόση του φυτικού αφεψήματος επηρέασε με ιστοειδικό τρόπο τις υπό μελέτη βιοχημικές παραμέτρους, καθώς παρατηρήθηκε αύξηση των επιπέδων της ανηγμένης γλουταθειόνης και μείωση της υπεροξείδωσης λιπιδίων στον ολικό εγκέφαλο (-παρ/δας) των ενηλίκων μυών, η οποία συνοδευόταν από αλλαγές στις επιμέρους εγκεφαλικές περιοχές της παρεγκεφαλίδας και του μεσεγκεφάλου ενώ ο εγκεφαλικός φλοιός ακολούθησε το πρότυπο του ήπατος και έδειξε να μην επηρεάζεται. Σε σχέση με την ενεργότητα των δυο ισομορφών του ενζύμου της AChE παρατηρήθηκε αναστολή στον ολικό εγκέφαλο (-παρ/δας) της πειραματικής ομάδας συγκρινόμενη με τους μάρτυρες, η οποία συνοδεύτηκε από μείωση στις επιμέρους περιοχές του εγκεφαλικού φλοιού, του ραβδωτού και του ιπποκάμπου. Το υδατικό εκχύλισμα του Sideritis clandestina subsp. clandestina παρουσίασε: 10% αντιχολινεστερασική δράση in vitro σε αντίθεση με τον αναστολέα της γαλανταμίνης ο οποίος ανέστειλλε το ένζυμο σε ποσοστό 85%. Τέλος, το φυτικό εκχυλίσματος σε συγκέντρωση 0,3mg/mL ανέστειλε τη συσσωμάτωση του Αβ πεπτιδίου σε ποσοστό περίπου 85%. Με βάση τα ανωτέρω το «τσάι του βουνού» επιδεικνύει τάση για αγχόλυση, ενισχύει την αντιοξειδωτική άμυνα, έχει αντιχολινεστερασικές ιδιότητες και επιφέρει ανασταλτικό αποτέλεσμα στη συσσωμάτωση του Αβ αμυλοειδούς. Οι δράσεις του αυτές οφείλονται πιθανόν στην πολυφαινολική του σύσταση και οι μηχανισμοί που εμπλέκονται χρήζουν περαιτέρω διερεύνησης. / “Mountain tea” (various species of Sideritis) has been traditionally consumed in Greece as a calmative and against common cold and allergies. The aim of the present study was to examine the neuroprotective role of the above plant and focus on: a) the effect of tea drinking in behavioral parameters of adult mice (fear/anxiety, learning and memory), b) the effect of tea drinking in antioxidative biochemical parameters of adult mice brain and liver, i.e. the concentration of reduced glutathione (GSH), the levels of lipid peroxidation and the activity of the two acetylcholinesterase (AChE) isoforms, and c) in vitro putative anticholinesterase and antiamyloid actions of Sideritis clandestina subsp. clandestina infusion. The beverage was provided (4g/100 mL, daily) for 40 days to adult male Balb-c/jice. Fear/anxiety was assessed by the measurement of i) the percentage of time spent in the open arms of the elevated plus maze apparatus and ii) the thigmotactic response (the tendency to remain close to vertical surfaces) of adult mice in an open-field. Learning and memory was assessed using the step-through passive avoidance task which is based on the obseravation that the experimental mice remember that a specific reaction has negative effects (electric foot shock). The oxidant/antioxidant properties of tea drinking was determined via measurement of a) the concentration of GSH (antioxidant marker), which is based on the formation of a fluorescent complex after the reaction of o-pthalaldehyde with glutathione and hystidyl compounds and b) the levels of malondialdehyde (MDA) (oxidant marker) by monitoring thiobarbituric acid reactive substance formation. The ex vivo and in vitro AChE activity was measured using the colorimetric method of Ellman where acetylthiocholine iodide was used as a substrate. Galantamine, a strong inhibitor of AChE, was used as a standard during in vitro determination of the enzyme activity. The effect of the infusion on amyloid-beta aggregation was studied in vitro with a thioflavine T - based fluorescence assay. Tea drinking caused statistically significant a) increase of the time percentage that animals spent into the open arms of the elevated plus maze apparatus and b) decrease of thigmotaxis time and increase of the time that animals entered to the central area of open field. Initial and Step-Through Latency showed no significant difference between two animal groups. The beverage also affected the biochemical parameters examined in the present study, in a tissue specific manner. More specifically, adult mice whole brain (-Ce) displayed increased reduced glutathione content and decreased lipid peroxidation levels compared to the controls. Similar changes were also observed in cerebellum and midbrain while cerebral cortex and liver were not affected. Regarding the activity of the two AChE isoforms, tea intake caused significant inhibition of these enzymes’ activity in whole brain (-Ce), compared with the control group. In agreement, cerebral cortex, striatum and hippocampus dispayed similar inhibition in both AChE isoforms activity after tea consuming. Sideritis clandestina subsp. clandestina infusion exhibited 10% in vitro anticholinesterase activity while galantamine inhibited the enzyme in a percentage of 85%. Moreover, tea infusion in a concentration of 0,3 mg/mL exhibited 85% inhibition of Ab1-40 fibrillogenesis in vitro, indicating, thus, strongly a putative antiamyloid action of Sideritis clandestina subsp. clandestinα. Conclusively, our results suggest that mountain tea infusion exhibits anxiolytic-like action, antioxidant and anticholintesterase properties and a strong, in vitro, inhibitory effect on amyloid-beta’s aggregation. These activities are most probably due to the polyphenols contained in Sideritis clandestina subsp. clandestina infusion; the underlying mechanisms need, though, further, in deep investigation.