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Intrinsic Exercise Capacity Affects Glycine and Angiotensin-Converting Enzyme 2 (ACE2) Levels in Sedentary and Exercise Trained RatsKlöting, Nora, Schwarzer, Michael, Heyne, Estelle, Ceglarek, Uta, Hoffmann, Anne, Krohn, Knut, Doenst, Torsten, Blüher, Matthias 20 October 2023 (has links)
Angiotensin-converting enzyme 2 (ACE2) has been identified as the cellular entry receptor
for the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High ACE2 tissue
expression and low glycine levels were suggested to increase susceptibility for SARS-CoV-2 infection
and increasing circulating ACE2 has been proposed as one possible strategy to combat COVID-19. In
humans, aerobic physical exercise induces an increase in plasma ACE2 in some individuals. However,
it is not clear whether glycine and ACE2 levels depend on intrinsic exercise capacity or on exercise
training. We used rats selectively bred for high intrinsic exercise capacity (HCR) or low exercise
capacity (LCR) and tested the influence of this genetic predetermination and/or aerobic exercise
on metabolites, ACE2 tissue expression and circulating ACE 2. ACE2 expression was measured in
different tissues in the sedentary animals and again after 4 weeks of high-intensity aerobic exercise in
both LCRs and HCRs. Sedentary HCRs exhibited significantly higher circulating ACE2 concentrations
compared to LCRs, but a lower expression of ACE2 in all investigated tissues except for adipose
tissue. Body weight was negatively correlated with serum ACE2 and positively correlated with ACE2
expression in the heart. Aerobic exercise caused a significant decrease in ACE2 expression in the
lung, heart, muscle, and kidney both in LCRs and HCRs. Our results suggest that ACE2 expression,
circulating ACE2 and glycine serum concentration are related to aerobic intrinsic exercise capacity
and can be influenced with exercise. These results may support the hypothesis that physically fit
individuals have a lower susceptibility for COVID-19 infection.
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Modulación de las funciones ionotrópica y metabotrópica del receptor nicotínico de acetilcolina α7 humanoChrestia, Juan Facundo 10 July 2023 (has links)
La supervivencia de los organismos superiores depende de que sus células se
organicen y actúen de manera sincronizada para cumplir funciones específicas, para lo
cual es fundamental la comunicación intercelular. Este proceso es básico para la vida de
todas las células, pero es la razón de ser en las neuronas que están especializadas en
recibir información, procesarla y comunicarla a otras células. En el sistema nervioso, la
principal forma de comunicación se realiza a través de la sinapsis química, en la que una
neurona libera un mensajero químico, el neurotransmisor, que es reconocido por un
receptor presente en otra célula permitiéndole responder al mensaje.
La acetilcolina es uno de los principales neurotransmisores utilizados por las
neuronas, y sus receptores, tanto metabotrópicos como ionotrópicos, están expresados
en muchos tipos celulares. Los receptores ionotrópicos de acetilcolina, llamados
receptores nicotínicos, son canales catiónicos pentaméricos que pertenecen a la familia
de receptores Cys-loop.
El receptor nicotínico de acetilcolina α7 es un homopentámero que exhibe
propiedades funcionales particulares fundamentales para su rol neuromodulador,
incluyendo la elevada permeabilidad al Ca2+ y la capacidad para transformar respuestas
ionotrópicas transitorias en eventos más sostenidos de señalización metabotrópica. Es
uno de los receptores nicotínicos más abundantes en el sistema nervioso, aunque también
se encuentra presente en otros tejidos. En el sistema nervioso central cumple un rol
importante en procesos de cognición, atención y memoria, al regular la liberación de
neurotransmisores, mediar la transmisión sináptica rápida y modular la excitabilidad
neuronal. Una disminución de su actividad se ha asociado con diversos desórdenes
neurológicos y neurodegenerativos, incluyendo esquizofrenia, autismo y enfermedad de
Alzheimer. El receptor α7 también se expresa en células no neuronales, tales como -entre
otras-, los astrocitos, la microglía, los linfocitos B y T, las células epiteliales, los
macrófagos, cumpliendo un rol importante en inmunidad, inflamación y neuroprotección.
Las acciones neuromoduladoras, neuroprotectoras y antinflamatorias sistémicas
del receptor nicotínico de acetilcolina α7 junto a sus propiedades únicas de activación,
desensibilización, permeabilidad al Ca2+ y rol dual ionotrópico-metabotrópico, lo han
convertido en un blanco farmacológico emergente muy importante en diversos
desórdenes neurológicos, neurodegenerativos e inflamatorios.
Este trabajo de tesis se basó en el estudio de los aspectos moleculares relacionados
a diferentes tipos de modulación de las funciones ionotrópicas y metabotrópicas del
receptor nicotínico de acetilcolina α7 humano. Para ello se utilizaron principalmente
técnicas electrofisiológicas a nivel de canal único y de corrientes macroscópicas, en
conjunto con análisis de proteínas por western blot y ensayos de movimiento de Ca2+
intracelular.
El capítulo I se centró en el estudio de la modulación de las funciones ionotrópicas y
metabotrópicas del receptor α7 por eventos de fosforilación/desfosforilación. Se
demostró que favorecer el estado desfosforilado de las tirosinas del dominio intracelular
de α7 potencia la actividad ionotrópica del receptor. A nivel de corrientes unitarias, el
efecto potenciador involucró un aumento en la frecuencia y duración de los episodios de
activación, mientras que a nivel de corrientes macroscópicas se manifestó por una
disminución en la velocidad de decaimiento de la corriente, y un aumento en la tasa de
recuperación desde el estado desensibilizado. Por el contrario, la desfosforilación de las
tirosinas tuvo un efecto negativo en la actividad metabotrópica del receptor, estudiada
por western blot a partir de la vía de ERK 1/2. Además, a diferencia de lo observado para
las tirosinas, las alteraciones en el estado de fosforilación de serinas y treoninas del
dominio intracelular no ocasionaron cambios importantes en la actividad ionotrópica de
α7 en las condiciones experimentales aquí utilizadas. En síntesis, los resultados
presentados en este capítulo ponen en evidencia que la fosforilación de las tirosinas, si
bien es absolutamente necesaria para la actividad metabotrópica de α7 mediada por la
vía ERK 1/2, actúa como un modulador negativo de la actividad ionotrópica del receptor.
El capítulo II abordó el estudio de la asociación funcional entre un fragmento
peptídico de la glicoproteína S del SARS-CoV-2 (Y674-R685) y el receptor nicotínico de
acetilcolina α7 humano. La asociación entre SARS-CoV-2 y los receptores nicotínicos fue
propuesta en forma de hipótesis al comienzo de la pandemia. Más adelante, simulaciones
de dinámica molecular mostraron que el fragmento Y674-R685 no solo tiene afinidad por
α7, sino que penetra profundamente en el bolsillo de unión a agonista del receptor. En
este capítulo, en primer lugar, se demostró que el fragmento Y674-R685 actúa como un
agonista silente de α7, ya que es capaz de provocar corrientes unitarias y macroscópicas
del receptor, pero solo en presencia de un modulador alostérico positivo. Por otro lado,
se demostró que Y674-R685 también ejerce una modulación negativa de α7, que se
evidenció por una profunda disminución, dependiente de la concentración, en la duración
de los episodios de activación de los canales potenciados y en la amplitud de las
respuestas macroscópicas provocadas por la acetilcolina. De esta manera, utilizando
distintos enfoques electrofisiológicos, se develó la existencia de una interacción funcional
entre el fragmento Y674-R685 de la glicoproteína S del SARS-CoV-2 y el receptor α7 que
proporciona las bases moleculares para seguir explorando la participación de los
receptores nicotínicos en la fisiopatología de la COVID-19.
El capítulo III se basó en el estudio del receptor α7 como blanco del cannabidiol, lo
cual resulta de gran interés debido al uso expandido de este fitocannabinoide para tratar
diferentes condiciones patológicas gracias a sus propiedades terapéuticas y a la ausencia
de efectos psicoactivos. Para ello se exploró el efecto del cannabidiol en las funciones
ionotrópicas y metabotrópicas de α7 mediante técnicas electrofisiológicas y ensayos de
movimiento de Ca2+ intracelular. En lo que respecta a las funciones ionotrópicas, se
demostró que el cannabidiol produce una rápida disminución de la actividad del canal a
nivel de corrientes unitarias evidenciada por la reducción en la frecuencia de los episodios
de activación. Este efecto fue dependiente de la concentración y se dio con una CI50 en el
rango submicromolar, lo que indica una potente modulación negativa. Por otra parte, el
cannabidiol también produjo una modulación negativa en la función metabotrópica de α7
que se evidenció por una marcada disminución en las respuestas de Ca2+ intracelular tras
la activación del receptor. Estos resultados demuestran que el cannabidiol ejerce una
modulación negativa de α7 de relevancia farmacológica que debe tenerse en cuenta a la
hora de evaluar los posibles usos terapéuticos del fitocannabinoide.
En conjunto, los resultados presentados en esta tesis amplían el entendimiento de
los aspectos moleculares relacionados con la modulación de las funciones ionotrópicas y
metabotrópicas del receptor nicotínico de acetilcolina α7 en distintas condiciones, a
saber, fisiológicas (eventos de fosforilación/desfosforilación), patológicas (fragmento
peptídico derivado de la glicoproteína S del SARS-CoV-2) y terapéuticas (cannabidiol). / The survival of higher organisms depends on the ability of their cells to be well
organized and to behave in a synchronized manner to fulfill specific functions for which
intercellular communication is of pivotal importance. This process is basic for the life of
all cells, but it is the raison d'être in neurons that are specialized in receiving information,
processing it, and communicating it to other cells. In the nervous system, the main form
of communication is carried out via the chemical synapse, in which a neuron releases a
chemical messenger, the neurotransmitter, which is identified by a receptor present in
another cell, allowing it to respond to the message.
Acetylcholine is one of the main neurotransmitters used by neurons, and its
receptors, both metabotropic and ionotropic, are expressed in many cell types. Ionotropic
acetylcholine receptors, called nicotinic receptors, are pentameric cation channels
belonging to the Cys-loop receptor family.
The α7 nicotinic acetylcholine receptor is a homopentamer with particular
functional properties critical to its neuromodulatory role, including high Ca2+
permeability and the ability to transform transient ionotropic responses into more
sustained metabotropic signaling events. It is one of the most abundant nicotinic
receptors in the nervous system, although it is also present in other tissues. In the central
nervous system, it plays an important role in cognition, attention, and memory, by
regulating the release of neurotransmitters, mediating rapid synaptic transmission, and
modulating neuronal excitability. A decrease in α7 activity has been associated with
various neurological and neurodegenerative disorders, such as schizophrenia, autism,
and Alzheimer's disease. The α7 receptor is also expressed in non-neuronal cells; namely,
astrocytes, microglia, B and T lymphocytes, epithelial cells, and macrophages, and plays
an important role in immunity, inflammation, and neuroprotection.
The neuromodulatory, neuroprotective, and systemic anti-inflammatory actions of
α7, together with its unique activating, desensitizing, Ca2+ permeability, and dual
ionotropic-metabotropic properties, have made the receptor a very important emerging
drug target in various neurological, neurodegenerative, and inflammatory disorders.
This P.D. thesis work was based on the study of molecular aspects related to
different types of modulation of the ionotropic and metabotropic functions of the human
α7 nicotinic acetylcholine receptor. To this end, electrophysiological techniques at the
single channel and macroscopic current levels were mainly used, as well as protein
analysis by western blot and intracellular Ca2+ movement assays.
Chapter I focused on the study of α7 receptor ionotropic and metabotropic function
modulation by phosphorylation/dephosphorylation events. It was shown that favoring
the dephosphorylated state of α7 intracellular domain tyrosine residues potentiates its
ionotropic activity. At the single-channel level, this potentiating effect involved an
increase in the frequency and duration of activation episodes, while at the macroscopic
level it was manifested by a decrease in the rate of current decay and by an increase in the
rate of recovery from the desensitized state. In contrast, tyrosine dephosphorylation had
a negative effect on receptor metabotropic activity, studied by western blot from ERK 1/2
pathway. In addition, unlike what was observed for tyrosine residues, alterations in the
phosphorylation status of serine and threonine residues present in the intracellular
domain did not cause any significant changes in α7 ionotropic activity under the
experimental conditions used. Summing up, the results collected in this chapter show that
tyrosine phosphorylation, although it is absolutely necessary for α7 metabotropic activity
mediated by ERK 1/2 pathway, acts as a negative modulator of receptor ionotropic
activity.
Chapter II focused on the study of the functional association between a peptide
fragment of SARS-CoV-2 S glycoprotein (Y674-R685) and human α7 nicotinic
acetylcholine receptor. The association between SARS-CoV-2 and nicotinic receptors was
hypothesized at the beginning of the pandemic. Later, molecular dynamics simulations
showed that the Y674-R685 fragment not only has affinity for α7 but also penetrates deep
into its agonist-binding pocket. In this chapter, it was firstly stated that the Y674-R685
fragment acts as a silent α7 agonist, since it is capable of triggering single-channel and
macroscopic currents, but only in the presence of a positive allosteric modulator. On the
other hand, it was shown that Y674-R685 also exerts α7 negative modulation, which was
evidenced by a profound concentration-dependent decrease in the duration of
acetylcholine-induced activation episodes from potentiated channels and macroscopic
responses. In this way, using different electrophysiological approaches, the existence of
functional interaction between SARS-CoV-2 S glycoprotein Y674-R685 fragment and α7
receptor was revealed, which provides the molecular bases to further explore nicotinic
receptor participation in COVID-19 pathophysiology.
Chapter III was based on the study of the α7 receptor as a target of cannabidiol,
which is of great interest due to the expanded use of this phytocannabinoid to treat
different pathological conditions thanks to its therapeutic properties and the absence of
psychoactive effects. To this end, the effect of cannabidiol on α7 ionotropic and
metabotropic functions was explored using electrophysiological techniques and
intracellular Ca2+ movement assays. Regarding ionotropic functions, it was shown that
cannabidiol produces a rapid decrease in single-channel activity evidenced by the
reduction in activation episodes frequency. This concentration-dependent effect occurred
with an IC50 in the submicromolar range, indicating a potent negative modulation. On the
other hand, cannabidiol also produced a negative modulation in α7 metabotropic function
that was evidenced by a marked decrease in intracellular Ca2+ responses after receptor
activation. These results demonstrate that cannabidiol exerts α7 negative modulation of
pharmacological relevance that must be taken into account when evaluating possible
therapeutic uses of the phytocannabinoid.
Taken together, the results presented in this thesis broaden the understanding of
molecular aspects related to the modulation of α7 nicotinic acetylcholine receptor
ionotropic and metabotropic functions under different conditions, namely physiological
(phosphorylation/dephosphorylation events), pathological (SARS-CoV-2 S glycoprotein
fragment) and therapeutic (cannabidiol).
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Longitudinal evaluation of post-COVID-19 conditionsNayyerabadi, Maryam 05 1900 (has links)
Depuis l'émergence de la pandémie de SARS-CoV-2 en décembre 2019, plus de 675 millions de cas confirmés ont été signalés dans le monde, dont 4,6 millions de cas au Canada uniquement. Bien que la plupart des individus récupèrent sans séquelles, 10 à 20 % des survivants signalent des symptômes persistants au-delà de quatre semaines après une infection par le SARS-CoV-2, tels que la fatigue, les altérations cognitives, la toux, l'anxiété, la dépression, la douleur thoracique et autres, connus sous le nom de COVID longue ou de condition post-SARS-CoV-2 (PCC). Par conséquent, la physiopathologie, le diagnostic et la prise en charge de la PCC sont devenus un axe de recherche majeur. Pour contribuer à la compréhension de la PCC, nous avons mené le projet IPCO (Institut de Recherches cliniques de Montréal (IRCM) Post-COVID-19 Research Clinic), en posant comme hypothèses 1 que les personnes infectés par le SARS-CoV-2 au Québec présenteraient des signes et symptômes fréquents et variés post-phase aiguë, affectant différents systèmes d'organes, et 2 Les niveaux élevés de D-dimères dans PCC ne sont pas pertinents pour les événements thromboemboliques 3 que Chez les individus atteints de la PCC, la vaccination contre la COVID-19 réduirait les symptômes de la PCC en diminuant l'inflammation. Pour évaluer ces hypothèses, nous avons recruté des participants âgés de plus de 18 ans, un à 18 mois après l'infection aiguë, présentant au moins un symptôme persistant, et programmé des visites de base et de suivi à 3-6 mois, 1 an et 2 ans post-infection aiguë. Chaque visite comprenait des évaluations cliniques, des prélèvements, des évaluations en laboratoire, des questionnaires sur l'alimentation et le bien-être, ainsi que des évaluations de la physiologie pulmonaire et cardiaque. Sur la base d'une étude allemande qui a catégorisé les symptômes du PCC et les individuals en trois groupes de sévérité, nous avons classé nos participants en trois niveaux de sévérité : non/légère (score du PCC <10,75), modérée (10,75 < score du PCC < 26,25) et sévère (score du PCC > 26,25). Cette thèse présente les résultats de trois sous-études IPCO.
Dans l'étude descriptive, nous avons observé que la fatigue, les problèmes de mémoire et les maux de tête étaient les symptômes de PCC les plus courants, la majorité de nos participants étant des femmes et ayant été traités en ambulatoire pendant la phase aiguë. Dans l'étude transversale, nous avons constaté des différences significatives dans les mesures de santé et de bien-être à tous les moments, mais aucune différence significative dans les résultats des tests physiologiques entre les groupes PCC non/léger, modéré et sévère. Dans l'étude longitudinale, les marqueurs de l'inflammation se sont améliorés au fil du temps, mais le taux métabolique basal et la masse grasse ont augmenté. Dans la deuxième étude, nous avons observé une forte prévalence de participants ayant des niveaux de D-dimères, qui n'étaient pas associés à des événements thromboemboliques, et aucune corrélation entre le niveau de D-dimères et les niveaux de cytokines et de chimiokines. Dans la troisième étude, nous avons observé que les participants vaccinés présentaient significativement moins de symptômes de PCC.
Notre étude fournit une meilleure compréhension de la physiopathologie du PCC et de l'effet de la vaccination sur le profil clinique et inflammatoire du PCC, ce qui pourrait aider à la conception d'outils de gestion clinique et de recherche futurs. / Since the emergence of the SARS-CoV-2 pandemic in December 2019, over 675 million confirmed cases have been reported globally, with 4.6 million cases in Canada alone. Although most individuals recover without residual disease, 10-20% of survivors report symptoms persisting beyond four weeks after SARS-CoV-2 infection, such as fatigue, cognitive impairments, cough, anxiety, depression, chest pain, and others known as long-COVID or post SARS-CoV-2 condition (PCC). Consequently, the pathophysiology, diagnosis, and management of PCC have become a significant focus of research. To contribute to the understanding of PCC, we conducted the IPCO (Institut de Recherches cliniques de Montréal (IRCM) Post-COVID-19 Research Clinic) project, hypothesizing that 1 SARS-CoV-2 infected individuals in Quebec would present frequent and varied signs and symptoms post-acute phase, affecting different organ systems, and that 2 high D-dimer level in PCC is irrelevant to thromboembolic events , and 3 in individuals with PCC, COVID-19 vaccination would decrease PCC symptoms by reducing inflammation. To evaluate these hypotheses, we enrolled participants aged >18 years, one to 18 months post-acute infection, with at least one persistent symptom, and scheduled baseline and follow-up visits at 3-6 months, 1 year, and 2 years post-acute infection. Each visit involved clinical evaluations, sampling, laboratory evaluations, diet and well-being questionnaires, and pulmonary and cardiac physiology evaluations. Based on a German study that categorized PCC symptoms and individuals into three severity groups, we classified our participants into three severity levels: non/mild (PCC score < 10.75), moderate (10.75 < PCC score < 26.25), and severe (PCC score > 26.25). This thesis reports the results of three IPCO studies.
In the descriptive study, we observed that fatigue, memory problems, and headaches were the most common PCC symptoms, with the majority of our participants being female and managed as outpatients during the acute phase. In the cross-sectional study, we noted significant differences in health and well-being measurements at all time points, but no significant difference in physiological tests' results between different severity groups. In the longitudinal study, markers of inflammation improved over time, but the basal metabolic rate and body fat increased. In the second study, we observed a high prevalence of participants having D-dimer levels in blood, which were not associated with thromboembolic events, and no correlation between D-dimer levels and blood cytokine/ chemokine levels. In the third study, we observed that vaccinated participants had significantly fewer PCC symptoms, fewer organ systems affected, higher well-being scores, and lower blood cytokine/chemokine levels than the non-vaccinated group. We also observed correlations between certain cytokines/chemokines, as well as between clinical parameters and certain cytokines/chemokines.
Our study provides a better understanding of the pathophysiology of PCC and effect of vaccination on the clinical and inflammatory profile of PCC, which could assist future research and clinical management tool design.
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COVID-19 Vaccination Coverage in Patients with Rheumatic Diseases in a German Outpatient Clinic: An Observational StudyKrasselt, Marco, Baerwald, Christoph, Seifert, Olga 02 June 2023 (has links)
Background: In the second year of the COVID-19 pandemic, highly effective and safe vaccines became available. Since patients with rheumatic diseases show increased susceptibility to infections and typical medications raise the risk of severe COVID-19, high vaccination coverage is of significant importance to these patients. Methods: Consecutive patients with different rheumatic diseases were asked for their vaccination status regarding COVID-19, influenza and Streptococcus pneumoniae during their routine consultations. Any reported vaccination was validated with their personal vaccination card and/or by reviewing the CovPass smartphone app. Reasons for not having a COVID-19 vaccination were documented. Results: A total of 201 patients (mean age 62.3 ± 14.1 years) were included, the majority of them (44.3%) with rheumatoid arthritis, followed by spondyloarthritis (27.4%) and connective tissue diseases (21.4%). Vaccination coverage for SARS-CoV-2 was 80.1%; 85.6% got at least the first vaccination shot. Both valid influenza and pneumococcus coverage were associated with a higher probability of SARS-CoV-2 vaccination (odds ratio (OR) 6.243, 95% confidence interval (CI) 2.637–14.783, p < 0.0001 and OR 6.372, 95% CI 2.105–19.282, p = 0.0003, respectively). The main reason for a missing SARS-CoV-2 vaccination (70%) was being sceptical about the vaccine itself (i.e., the subjective impression that the vaccine was not properly tested and fear of unwanted side effects). Conclusions: Vaccination coverage against SARS-CoV-2 is high in patients with rheumatic diseases. Nevertheless, there are unmet needs regarding vaccination education to further increase vaccination rates.
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Factors Associated with COVID-19 Vaccination Decisions Among Florida NursesKoo, Jacey G 01 January 2023 (has links) (PDF)
At the beginning of 2020, the SARS-CoV-2 virus, known more commonly as COVID-19, created a pandemic. To slow its spread, healthcare workers were heavily encouraged to vaccinate themselves. However, nurses have been less likely to be vaccinated against COVID-19 than physicians. Four common themes have been associated with vaccine hesitancy among nurses, namely certain demographic variables (e.g., younger age and female sex), fears of the vaccine, conspiracy theories and news sources, and medical and psychological histories that pertain to receiving the COVID-19 vaccine. Thus, this study aimed to identify whether these factors apply to Florida nurses' decisions to get vaccinated after the height of the pandemic. To approach this problem, sixty-five participants were surveyed through a Qualtrics cross-sectional questionnaire. The results revealed that approximately 18.5% of participants were not vaccinated. Trends in the data revealed that older age and a postgraduate education level were associated with receiving the vaccine. Non-vaccinated participants had less confidence in the vaccine's ability to reduce the risk of hospitalization, death, and infection, and they had a stronger fear of side effects and the vaccine's rapid development. Several vaccinated and non-vaccinated participants also believed vaccine conspiracy theories, such as that vaccine safety data is falsified. Many non-vaccinated nurses also received SARS-CoV-2 information from social media or their patients, whereas many vaccinated nurses received information from government news sources or physicians. Non-vaccinated nurses also tended to have more discomfort towards hypodermic injections than vaccinated nurses. These conclusions are generalizable to the nurses of this study and may not be generalizable to all nurses. However, because nurses are on the frontlines of the healthcare field and have an essential role in informing the public about health, the results of this study can help inform vaccine education interventions should a future pandemic occur.
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Anestesisjuksköterskors upplevelser av att vårda intensivvårdspatienter med covid-19Hedehag Damberg, Agnes, Jonsson, Anna January 2021 (has links)
Bakgrund: Under slutet av 2019 bröt en ny epidemi ut med ett nytt coronavirus som orsakar feber och luftvägsbesvär. Under våren 2020 benämndes epidemin som en pandemi. Resurser har fördelats för att kunna ta hand om det stora antalet människor som insjuknat i covid-19. Anestesisjuksköterskor har under pandemin omplacerats för att vårda intensivvårdspatienter med covid- 19. Intensivvård och anestesisjukvård är två olika men nära besläktade utbildningar. Syfte: Syftet med denna undersökning var att beskriva anestesisjuksköterskors upplevelse av att vårda intensivvårdspatienter med covid-19. Metod: En kvalitativ design med deskriptiv ansats. Åtta anestesisjuksköterskor som uppfyllde urvalskriterierna har intervjuats. Data har analyserats med kvalitativ innehållsanalys. Resultat: Anestesisjuksköterskorna upplevde det som att bli inkastad i något okänt med bristande introduktion. Stora delar av omvårdnaden lämnades över till undersköterskor erfarna inom intensivvård som hjälpte till att leda omvårdnadsarbetet runt patienten. Ett gott samarbete bland kollegor var viktigt för att känna trygghet. Anestesisjuksköterskorna upplevde stress och oro över att arbeta med en ny patientgrupp, medicinteknik och läkemedelshantering. Anestesisjuksköterskorna kände stolthet över att kunna hjälpa till i en pandemi och göra sitt bästa. De nämnde det som positivt att de hade utvecklats i sin yrkesroll. Diskussion: Arbetets fynd talar om en påfrestande arbetssituation präglad av mycket stress och oro på grund av avsaknad av intensivvårdsspecifik kompetens. Tidigare forskning beskriver att kompetens är ett fenomen som påverkas av utbildning, arbetslivserfarenhet och kvalificering för det speciella yrkesutövan. Slutsats: Patientsäkerheten och omvårdnad av patienten kan brista när personalen saknar kompetens och tid för att utöva sitt arbete. Politiker och chefer behöver mobilisera en sjukvård som klarar av en ny pandemi för att kunna upprätthålla god patientvård- och säkerhet. Nyckelord: Anestesisjuksköterska, omvårdnad, covid- 19/SARS- CoV-2, pandemi, patientsäkerhet. Keywords: Nurse anaesthetists, nursing, covid-19/SARS-CoV-2, pandemic, patient safety
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Concomitant Guillain-Barre Syndrome with COVID-19Morongell, Skylar A 01 January 2021 (has links)
The current Coronavirus disease 2019 (COVID-19) outbreak, caused by a virus called severe acute respiratory syndrome 2 (SARS-CoV-2), has become a global health emergency. Recent findings in case studies assert that the transmigration of SARS-CoV-2 to the nervous system implicates severe neurotropic pathologies, including the onset of the rare autoimmune disease called Guillain-Barré syndrome (GBS). GBS is recognized as several disorders characterized by immune-mediated polyradiculoneuropathy, which is typically preceded by an infection or other immune stimulation. The symptoms of GBS initially present as acute symmetrical ascending paresthesia, weakness, and paralysis.
This meta-analysis serves to help understand the predisposing factors (such as gender, age, comorbidities) and the clinical features of COVID-19- induced GBS. Most patients affected were 40 years or older and comprised 78.2% of all the cases. Males comprised most of the cases (62.8%; n=76). The patient mortality was 9.1%, intensive care unit (ICU) admission was 46.6%, and the need for mechanical ventilation was 35.8%. It was found that concomitant GBS and COVID-19 patients most often presented with increased cerebral spinal fluid (CSF) protein levels (88%; n=106), hyporeflexia or areflexia (87.6%) (n=106), lower limb strength and sensation impairment (91.7%; n=111), upper limb strength and sensation impairment (83.5; n=101), and somatic sensation impairment (73.6%; n=89).
It is postulated that COVID-19 triggers the onset of GBS through a “cytokine release storm” (CRS) that occurs in the early stages of the disease. The same cytokines and chemokines involved in this CRS caused by COVID-19 contribute to the onset of GBS. Predisposing factors which influence this concomitance include male gender and older age. Most of the reported symptoms included abnormal limb functions (including paresthesia, weakness, and paralysis) and absent or weak deep tendon reflexes. The most common variant of GBS observed was AIDP, and the most significant laboratory finding among patients was high CSF protein levels.
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Evaluation of Interactions of COVID Nonstructural Proteins 3, 5, and 6 With Human Proteins and Potentially Therapeutic MoleculesHuitsing, Jessica 01 January 2022 (has links)
The COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2, or SARS-CoV-2, has been ongoing for over two years. The virus spreads easily and is more unpredictable than well-known viruses like the flu, making it important to have reliable combative measures before we fully drop non-vaccine preventive actions, like mask-wearing.Therefore, we used computational protein modeling to investigate the interactions of three nonstructural proteins (abbreviated Nsp) encoded in the viral RNA genome– Nsp3, Nsp5, and Nsp6 – which are involved in the viral life cycle, with human P-type polyamine transporting ATPases ATP13A2 and ATP13A3, whose disease symptoms when mutated mimic certain COVID-19 complications. Understanding these interactions can help shed light on the mechanism of unexpected symptoms seen in COVID-19 and provide an avenue through which to treat infections. Additionally, papain-like protease (PLpro) and 3-chymotrypsin-like protease (3CLpro), which correspond to Nsp3 and Nsp5, respectively, are highly conserved between SARS-CoV and SARS-CoV-2 and thus make good potential drug targets due to their active sites and presumable lower ability to tolerate mutations (reducing the likelihood of treatments becoming ineffective), although the potential effects on the human proteasome would need to be further investigated. In addition, Nsp6 may help the virus evade host defenses by limiting the ability of autophagosomes to deliver viral particles to lysosomes, so limiting its interactions may increase the ability of the host cell to target its viral invader. One compound in particular, Haloperidol, showed promising results; predicted docking (via computational molecular docking software) to Nsp6 alone, as well as to Nsp6-heteroprotein complexes suggested strong binding, indicating a potential strong interaction that could impact the viral protein function and thus the viral life cycle.
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The Association Between Obesity And COVID-19: An Analysis Of Risk FactorsHakim, Talib H 01 January 2022 (has links)
The purpose of this study was to focus on whether solely obesity (measured by BMI) leads to an increased risk of COVID-19 mortality in terms of excess in-hospital deaths. As the grim milestone of one million deaths in the United States from COVID-19 was reached, one could assume the disease would continue to coexist with humanity in the long term. While vaccination continued to limit the spread of COVID-19, it was essential to investigate risk factors that may exacerbate the severity of the illness in humans. Obesity is already a global pandemic affecting 40% of Americans and over 650 million people worldwide. Obesity is connected to an entire range of clinical conditions including some of the leading causes of death worldwide making it a more generalizable statistic. Furthermore, the purpose of this study was to conduct a systemic review of major risk factors between obesity and COVID-19, and this analysis shall ascertain which factors have the most predictive value in determining mortality and severity of the condition. Ten research studies of 3,780,926 COVID-19 patient cases were included. Meta-analysis results indicate a pooled OR of 0.93 (0.71-1.23, p = 0.627) for in-hospital mortality of obese patients relative to non-obese patients when adjusted for confounders. All comorbidities associated with the development of severe disease were found to have an equal chance of leading to mortality. In other words, obesity did not lead to a statistically significant risk of dying from COVID-19.
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Långvariga effekter av COVID-19 på diffusionskapaciteten i lungorna - en litteraturstudie / Long-term effects of COVID-19 on lung diffusion capacity – a literature studyTan, Evelina January 2021 (has links)
I december 2019 i Wuhan, Kina, blev en grupp personer infekterade av det nya viruset SARS-CoV-2 som inducerade sjukdomen COVID-19. Sjukdomen påverkar lungfunktionen och studier har visat att långvarig påverkan på lungfunktionen kan förekomma. Syftet med den aktuella studien var att undersöka långvariga effekter av COVID-19 på diffusionskapaciteten i lungorna. Studier som undersökte diffusionskapaciteten, mätt med kolmonoxid (DLCO), hos patienter minst 30 dagar efter insjuknande i COVID-19 samlades in och analyserades. Resultatet visade att en stor del av deltagarna hade DLCO <80% av det förväntade värdet. Det visade även att diffusionskapaciteten kan vara nedsatt upp till sex månader efter utskrivning från sjukhus. Då COVID-19 är en ny sjukdom var den aktuella studien begränsad av antalet studier inom området. Framtida studier bör undersöka effekterna av COVID-19 på lungfunktionen under ett längre tidsspann. Slutsatsen i den här litteraturstudien var att COVID-19 påverkar diffusionskapaciteten i lungorna. Långvariga nedsättningar i diffusionskapaciteten har, än så länge, kunnat påvisas upp till sex månader efter sjukdom. Allvarligt insjuknande i COVID-19 ökar risken för långvariga DLCO -nedsättningar.
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