The egress pathway of herpes simplex virus-1 (HSV-1) is a complicated process mediated by co-ordinated activity of several virus glycoproteins. The virions are first assembled and enveloped at trans-Golgi-network (TGN) or endosome membranes and then travel through a guided pathway that is directed towards the cell adherent points for secretion. Once secreted the vast majority of virions remain associated with the extracellular membrane of cells and very few free virions are released into the culture medium (< 1%). The mechanisms that mediate both the targeted secretion of newly assembled virions at cell contact points and post-secretion attachment of virions with the extracellular surface of cells are poorly understood, and were the topics of this research. In this thesis, an HSV-1 passage mutant of increased virion secretion phenotype had been studied. Genome sequencing of the mutant virus identified mutations in three viral envelope proteins. Study of recombinant viruses that were constructed based on those three mutations revealed that a single amino acid change in glycoprotein I (gI) of glycine to arginine at residue 39 is responsible for the increased release of virus. The result suggests the principal effect of this mutation is to modify the secretory pathway used by virions during their release from infected cells. Data also suggests a role of gC in the attachment of virions to the extracellular surface of cells after egress. In the context of HSV-1 envelopment and egress glycoprotein E (gE), which forms a heterodimeric complex with gI (gE/gI), is known to be important. The gE/gI complex has been shown to interact with many tegument proteins and have a redundant role in secondary envelopment. The gE/gI complex has been also proposed to colocalise with various cellular components and sort the nascent virions to cell contact points. However, there is little understanding of the cellular proteins that gE/gI interact with, or the mechanisms that mediate targeted secretion of virions. This research has identified a novel interactome of gE/gI by mass-spectrometric analysis utilising stable isotope labelling with amino acids in cell culture (SILAC) medium. Among the cellular interactome obtained, Nipsnap1 was validated by co-precipitation assays from both infected and transfected cells, and furthermore using cell free systems, suggesting gE and Nipsnap1 directly interact. Nipsnap1 and its homologue Nipsnap2 have been proposed to contribute in vesicle transport and membrane fusion in cells. Using CRISPR-Cas9 technology these proteins were knocked out in a keratinocyte cell line (HaCaT) to investigate their role in HSV-1 egress. However, little or no effect on HSV-1 egress could be observed upon loss of either or both of these proteins suggesting the biological significance of gE-Nipsnap1 interaction may not be directly linked to any egress function of gE/gI. Two further interesting 'hits' from the gE/gI interactome were interferon-induced transmembrane protein type-2 (IFITM2), a virus restriction factor, and Myoferlin that has a putative role in endocytic vesicle recycling. This study could validate gE-Myoferlin interaction and co-localisation in infected or transfected cells however, functional significance of this interaction remains to be determined. Overall, the research of this thesis has provided a better understanding of the role of the gE/gI complex in HSV-1 egress and investigated the role of some interesting cellular proteins in the context of virion egress.

Like all other viruses, a successful egress of functional particles from infected cells is a prerequisite for foamy virus (FV) spread within the host. The budding process of FVs involves steps, which are shared by other retroviruses, such as interaction of the capsid protein with components of cellular vacuolar protein sorting (Vps) machinery via late domains identified in some FV capsid proteins. Additionally, there are features of the FV budding strategy quite unique to the spumaretroviruses. This includes secretion of non-infectious subviral particles and a strict dependence on capsid-glycoprotein interaction for release of infectious virions from the cells. Virus-like particle release is not possible since FV capsid proteins lack a membrane-targeting signal. It is noteworthy that in experimental systems, the important capsid-glycoprotein interaction could be bypassed by fusing heterologous membrane-targeting signals to the capsid protein, thus enabling glycoprotein-independent egress. Aside from that, other systems have been developed to enable envelopment of FV capsids by heterologous Env proteins. In this review article, we will summarize the current knowledge on FV budding, the viral components and their domains involved as well as alternative and artificial ways to promote budding of FV particle structures, a feature important for alteration of target tissue tropism of FV-based gene transfer systems.

Like all other viruses, a successful egress of functional particles from infected cells is a prerequisite for foamy virus (FV) spread within the host. The budding process of FVs involves steps, which are shared by other retroviruses, such as interaction of the capsid protein with components of cellular vacuolar protein sorting (Vps) machinery via late domains identified in some FV capsid proteins. Additionally, there are features of the FV budding strategy quite unique to the spumaretroviruses. This includes secretion of non-infectious subviral particles and a strict dependence on capsid-glycoprotein interaction for release of infectious virions from the cells. Virus-like particle release is not possible since FV capsid proteins lack a membrane-targeting signal. It is noteworthy that in experimental systems, the important capsid-glycoprotein interaction could be bypassed by fusing heterologous membrane-targeting signals to the capsid protein, thus enabling glycoprotein-independent egress. Aside from that, other systems have been developed to enable envelopment of FV capsids by heterologous Env proteins. In this review article, we will summarize the current knowledge on FV budding, the viral components and their domains involved as well as alternative and artificial ways to promote budding of FV particle structures, a feature important for alteration of target tissue tropism of FV-based gene transfer systems.

Made available in DSpace on 2016-04-04T18:32:31Z (GMT). No. of bitstreams: 1 Angela Paiva.pdf: 360703 bytes, checksum: 90e569d0e8e0620a7252ff03b6a42c50 (MD5) Previous issue date: 2006-06-27 / This paper is according to the research line Studies on Evaluation . The paper aims to evaluate, by hearing egress students from the program of Post-Graduation strictosensu in Education of PUC-Campinas, from 1993 to 2004, the contributions brought by the course to their trajectory of personal and professional lives and to their institutional insertion. As a methodology, the qualitative research was used, through the application of a questionnaire sent via e-mail to 135 students of that period. Only 56 students replied. First of all, the Project of the Program of Post Graduation strictosensu was analyzed; then, other voices were heard such as the ones from External Evaluation Organs (CAPES) and the ones from the egress students. From that, a significance net was built, justifying the importance degree of an evaluatory, dynamic and shared process. Finally, the present research aims to give subsidies to the Program, suggesting changes that may be necessary in order to maintain its pedagogical propositions according to the transforming challenges of contemporary society. The studied information was debated to suggest strategies that may lead to a shared, dynamic internal evaluation, opened to the academic community and that can show a formative character to the evaluation process. Thus, the strengthening and improvement of the Program and its members can be reached. The trajectory of the egress student after leaving the course and the development of his/her qualification process were focused. It was noticed that values built during the course allowed most of the egresses of the given period to excel in subjects concerned to Education, mainly in higher education. The engaged and earnest characteristics of this 15-yearold program, as well as of its members were confirmed. / Este trabalho est? inserido na linha de pesquisa Estudos em Avalia??o . Objetivamos avaliar, por meio da voz dos egressos do programa de P?s-Gradua??o Stricto-sensu em Educa??o da PUC-Campinas de 1993 a 2004, as contribui??es que o curso trouxe em sua trajet?ria de vida pessoal, profissional e sua inser??o institucional. Usamos como metodologia, a pesquisa qualitativa, com aplica??o de um question?rio, enviado, via e-mail aos 135 egressos do per?odo e obtivemos 56 retornos. Ao analisarmos o Projeto do Programa de P?s-Gradua??o Stricto-sensu em Educa??o cotejamos outras vozes como dos ?rg?os de avalia??o externa (CAPES) e a dos egressos, construindo uma rede de significados justificando o grau de import?ncia de um processo avaliat?rio din?mico e participativo. A partir desse encontro objetivamos ainda subsidiar o pr?prio programa sobre as mudan?as que se fizerem necess?rias para manter sua proposta pedag?gica sintonizada com os desafios transformadores da sociedade contempor?nea. As informa??es analisadas s?o discutidas visando sugerir estrat?gias as quais poder?o culminar com uma din?mica de avalia??o interna participativa e aberta ? comunidade acad?mica imprimindo o car?ter formativo ao processo avaliativo visando o aperfei?oamento e fortalecimento do Programa e de seus membros. Focamos a trajet?ria do egresso ap?s a conclus?o do curso e os desdobramentos de seu processo de capacita??o, o que possibilitou-nos perceber que os valores constru?dos pelo curso permitiram que a maioria dos egressos do per?odo analisado se sobressa?sse nas quest?es ligadas a educa??o principalmente no tocante ao ensino superior, comprovando o car?ter s?rio e comprometido dos profissionais que constitu?ram estes 15 anos de vida do programa.

O sistema de pós-graduação trouxe contribuições para as universidades, principalmente no âmbito da melhoria da qualidade do ensino e da produção de conhecimento. Implantada na década de 60, com o propósito de qualificar profissionais, maioria docentes da própria universidade, visava a ampliar sua formação para a pesquisa e, consequentemente, incentivar a produção de conhecimento. Neste movimento, docência e pesquisa foram fortalecidos. Ao final da década de 90, a CAPES reconhece o Mestrado Profissional - stricto senso - como modalidade de pós-graduação. Com foco nos profissionais atuantes no mercado de trabalho, propõe sua aproximação com a pesquisa, com o compromisso de alcançar e modificar o ambiente de trabalho. Paralelamente, em 1990, é fundado o Hemocentro do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Com proposta inicial de assistência e atendimento ao público, este centro de saúde conviveu com a pesquisa realizada na academia, possibilitando conciliar a produção de conhecimento com a prática desenvolvida em sua rotina. Em 2012, inaugura o mestrado profissional em Hemoterapia e Biotecnologia, com o objetivo de formar e qualificar profissionais desta área. Neste trabalho, objetivou-se estudar este programa, com a proposta de investigar os mecanismos de interação estabelecidos entre o centro de pesquisa e as unidades de trabalho parceiras, a partir da perspectiva dos egressos (1ª e 2ª turmas). Por ter um caráter exploratório e inicial, buscou-se trabalhar com indicadores exclusivos da modalidade profissional, permitindo fortalecer o acompanhamento e avaliação do programa oferecido, principalmente àqueles relacionados à aplicabilidade dos projetos e o impacto do curso realizado para a atuação profissional do egresso. Dessa forma, o presente trabalho é um estudo exploratório-descritivo com abordagens quantitativas e qualitativas, com seis tópicos principais de investigação: o programa oferecido; os projetos de pesquisa realizados; as produções intelectuais; os desdobramentos da convivência em grupo de profissionais de diferentes estados brasileiros; as contribuições do orientador para o desenvolvimento dos projetos de pesquisa e as contribuições decorrentes da aproximação dos alunos com a pesquisa. Com característica de formação específica e direcionada, com possibilidade de reunir profissionais da área da Hemoterapia de nove regiões do Brasil, o programa tem permitido alcançar objetivos previstos na modalidade profissional (stricto sensu) e almejados pelo projeto de criação de 2011. Parte destes resultados deve-se principalmente à três mecanismos de interação identificados: o projeto de pesquisa, a convivência em grupo de profissionais e o orientador, cada um com contribuições específicas para o processo de formação e aprendizagem dos alunos, permitindo alcançar mudanças nas unidades de trabalho e nas condutas e competências profissionais. Essa primeira caracterização da proposta permite criar um ponto de comparação para o programa recém implantando e que passará por sua primeira avaliação quadrienal em 2017. Dificuldades e limitações também foram diagnosticadas e necessitam ser revistas, reestruturadas e trabalhadas, como o conteúdo prático do curso, o quadro de profissionais técnico-administrativos e o tempo de titulação, com média de 35,4 meses, que exige reflexão. O acompanhamento regular dos egressos e a criação de uma plataforma contínua de construção de dados, permitirá intervir e ajustar o programa, possibilitando perceber fases de amadurecimento e crescimento, com especial atenção aos indicadores relacionados à modalidade profissional. / The postgraduate system brought contributions to universities, mainly for the improvement in the quality of education and knowledge production. Established in the decade of 1960s, with the purpose of qualifying professionals, mostly professors of the university itself, it aimed at expanding their training in research and, as a result, encouraging knowledge production. In this sense, teaching and research have been strengthened. By the end of the decade of 1990, CAPES recognized the Professional Master - stricto sensu - as a postgraduate modality. Focusing on the professionals in business, it proposes an approach to research, with the commitment of reaching and modifying work environment. In parallel, in 1990, the Blood Center of the Clinical Hospital of the Ribeirão Preto Medical School of the University of São Paulo was founded. With the initial proposal of providing assistance and care to the population, this health center has lived with the research carried out in the academy, what enabled to reconcile knowledge production with the practice developed in its daily routine. In 2012, it launched the professional master in Hemotherapy and Biotechnology, with the aim of training and qualifying professionals in this field. In this work, the objective was to study this program, proposing to investigate the mechanisms of interaction established between the research center and partner work units, from the perspective of egress students (1st and 2nd classes). Because it has an exploratory and initial character, we planned to work with indicators exclusive to the professional modality, allowing to strengthen the follow-up and evaluation of the program offered, mainly those indicators related to the applicability of projects and to the impact of the course done on the professional act of the egress student. Therefore, the present work is an exploratory-descriptive study using quantitative and qualitative approaches, with six major investigation topics: the program offered; the research project developed; intellectual production; the unfolding of the experience of groups of professionals from different states of Brazil; the contribution of the supervisor for the development of research projects; and the contribution resulting from the approach of students to research. With the features of specific training and possibility of gathering professionals of the area of Hemotherapy from the nine regions of Brazil, the program has enabled to accomplish the objectives expected in the professional modality (stricto sensu) and established in the project creation in 2011. Part of these results is owed primarily to three mechanisms of interaction identified: the research project, experience of living as a group, and supervisor, each one with specific contributions to the process of students\' training and learning, enabling to achieve changes in work units and in professional conduct and competences. This first characterization of the proposal allows to create a comparison point for the recently implemented program, which will go through its first four-yearly assessment in 2017. Difficulties and limitations have also been diagnosed and must to be reviewed, restructured and managed, such as the practical content of the course, the chart of technical administrative professionals and the time for titling, with an average of 35.4 months, what demands some thoughts. The regular follow-up of egress students and creation of a continuous platform for data construction will allow to intervene and adjust the program, making possible to notice maturation and growth phases, with special attention to indicators related to the professional modality.

Made available in DSpace on 2015-03-05T19:13:43Z (GMT). No. of bitstreams: 0 Previous issue date: 17 / Nenhuma / Este estudo foi desenvolvido com o objetivo de conhecer o perfil pessoal, acadêmico e profissional de egressos dos cursos de ciências contábeis de Instituições de Ensino Superior do Rio Grande do Sul. Para tal foi conduzida uma pesquisa de campo mediante encaminhamento de questionário por meio eletrônico, respondido por uma amostra de 448 egressos, sendo 242 do sexo masculino e 206 do sexo feminino, que fizeram seus registros profissionais no Conselho Regional de Contabilidade do Rio Grande do Sul no período compreendido entre 1996 e 2005. Como principais resultados podem se destacados: a) a maioria optou pelo curso por vocação e por representar melhores oportunidades de emprego; b) um número razoável de egressos está engajado num processo de educação continuada, o que lhes permite manter-se atualizados e oferecer serviço de qualidade aos usuários; c) a maior parte se dedica à prestação de serviços contábeis na condição de empregados, merecendo destaque a atuação na área de controladoria, revelando uma mudanç / This study aims for a better understanding of the personal, academic and professional profile of egress students from Higher Education Institutions of Rio Grande do Sul. A field research was carried out through a survey sent by e-mail to a sample of 448 professionals, 242 males and 206 females certified by the Regional Council of Accountancy of Rio Grande do Sul between 1996 and 2005. The main outcomes indicate that: a) the majority chose this professional based on vocation and also on the fact that it represents better job opportunities; b) a considerable number are engaged in some kind of continued educational process, in order to update and improve the quality of services; c) a great number dedicate to accounting services as employees, mainly as controllers, which points to a change in the professional profile, as it tends to approach management; d) there is a good level of satisfaction regarding profession and salary, bearing in mind the variation of 7 to 15 years in the respondents’ working time experien

Made available in DSpace on 2016-08-02T21:42:36Z (GMT). No. of bitstreams: 1 AlfredoLucato.pdf: 645983 bytes, checksum: db2006c28784a5e97c0ad9e3482fd865 (MD5) Previous issue date: 2014-06-11 / The Higher Programs of Technology, known in Brazil as CST, are a specific type of higher education (Technologist), with its own characteristics, including the focus on specialization of their curricula and shorter duration aiming at a faster training. This kind of graduation has been widespread in the country since the mid-1990s, after the enactment of the law nº 9.394/96 - LDB, and by a series of decrees, orders and advices of the MEC / CNE. Among the outstanding main goals and objectives of the PNE for higher education, it may highlighted: diversified higher education system to meet the specific demands of clienteles training; linkage between curriculum and the labor market; financing and management; creating policies for access to higher education to facilitate the admission of students from vulnerable educational groups; increasing the of offer for higher education for the aged group 18-24 years. This study aims to reflect and discuss the importance of the CSTs in professional education of Brazilian workers through the expectation of PNE. The study is based on qualitative method, an exploratory and descriptive research, characterized as a single case study, through field research conducted with students and graduates of the course of Industrial Electronics, Faculty of Technology Senai Anchieta, in the city of Sao Paulo. To achieve its objectives, the study was divided into two stages: the first analyzes the variables that characterize the profile of the student in training in order to identify their perceptions regarding the choice by CST, knowledge about this type of education and future expectations of the profession. In the second part, the study analyzes the perception of graduate about the skills and competencies acquired during training, their degree of satisfaction with salary and career path, and the Technologist professional acceptance by the labor market. In a study conducted with students in training it was found that the dropout rates of the first classes were high, being identified as the main causes difficulties in reconciling study schedule with work and family economic hardship. The profile of the student training in IES under study is mostly the age group between 18 and 24 years; males; egress of secondary education done in public schools; resident in peripheral and natural neighborhood of the city RMSP; the student himself is responsible for payment of tuition; no third party interference in their choice of CST; have favorable perceptions regarding future employability as technologist. A survey of graduates showed that they have a favorable perception of the training received, except the fact that they had not attended courses that develop managerial skills and business; regarding the employability and career prospects were concerned in professional development technologist by the labor market; reported being dissatisfied about salary received; regarding continuing education be recognized that the process that will keep updated professionally. As a conclusion of this study, it is recommended to probing other possible causes of truancy, through a research with students from other courses with different thematic axes and public IES. It is pointed-out also the need to offer in the curriculum proposal of the IES under study, apart from technical disciplines, others in order to develop skills in people management and business management. / Os Cursos Superiores de Tecnologia são um tipo específico de graduação (tecnólogo), com características próprias, entre as quais, a focalização na especialização dos seus currículos e duração mais curta objetivando uma formação mais rápida. A graduação tecnológica vem sendo amplamente difundida no país desde a segunda metade da década de 1990, após a promulgação da lei no 9.394/96 LDB, e por uma série de decretos, portarias e pareceres do MEC/CNE. Entre as principais metas e objetivos do PNE para a educação superior, se destacam: diversificação do sistema superior de ensino para atender clientelas com demandas específicas de formação; articulação entre currículo e mercado de trabalho; financiamento e gestão; criação de políticas de acesso à educação superior que facilitem o ingresso de alunos provenientes de grupos de maior vulnerabilidade educacional; crescimento da oferta de educação superior para a faixa etária de 18 a 24 anos. O presente estudo tem por objetivo refletir e debater a contribuição dos CSTs na educação profissional do trabalhador brasileiro dentro das perspectivas do PNE. O estudo se fundamenta no método qualitativo, com base numa pesquisa exploratória e descritiva, caracterizada como estudo de caso único, através da pesquisa de campo realizada com alunos em formação e com egressos do Curso de Eletrônica Industrial da Faculdade de Tecnologia Senai Anchieta, na cidade de São Paulo. Para alcançar seus objetivos, o estudo foi dividido em duas etapas: a primeira faz uma análise das variáveis que caracterizam o perfil do aluno em formação buscando identificar sua percepção quanto à escolha pelo CST, conhecimento sobre essa modalidade de ensino e as expectativas futuras da profissão. Na segunda parte, o estudo faz uma análise da percepção do egresso acerca das habilidades e competências adquiridas durante a formação, o grau de satisfação com salário e plano de carreira, e a aceitação profissional do tecnólogo pelo mercado de trabalho. No estudo realizado com alunos em formação constatou-se que os índices de evasão escolar das primeiras turmas do curso foram elevados, sendo apontadas como principais causas as dificuldades de conciliação entre o horário de estudo com a do trabalho e as dificuldades econômicas familiares. O perfil do aluno em formação na IES em estudo é majoritariamente da faixa etária entre 18 e 24 anos; do gênero masculino; egresso do ensino médio feito em escola pública; morador em bairro periférico e natural de cidade da RMSP; o próprio aluno é responsável pelo pagamento das mensalidades; não houve interferência de terceiros na sua escolha pelo CST; tem percepção favorável quanto à empregabilidade futura como tecnólogo. A pesquisa com egressos apontou que os mesmos tem percepção favorável quanto à formação profissional recebida, exceto o fato de não terem atendido disciplinas que desenvolvessem competências gerenciais e de negócios; quanto à empregabilidade e perspectivas de carreira mostraram preocupação na valorização profissional do tecnólogo pelo mercado de trabalho; declararam estar insatisfeitos quanto ao salário recebido; quanto à educação continuada reconheceram ser esse o processo que os manterão atualizados profissionalmente. Como conclusão da pesquisa, há recomendação para estudos futuros na sondagem de outras possíveis causas da evasão escolar com alunos dos cursos com outros eixos temáticos e de IES públicas. Igualmente, aponta-se para a necessidade da proposta curricular da IES em estudo oferecer, além das disciplinas técnicas, outras que desenvolvam competências e habilidade em gestão de pessoas e negócios.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-03-22T17:55:51Z No. of bitstreams: 1 rosaurarodriguestoledo.pdf: 1086955 bytes, checksum: e74c67a5a953194e0036f4f57bfc691d (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2016-04-24T02:26:52Z (GMT) No. of bitstreams: 1 rosaurarodriguestoledo.pdf: 1086955 bytes, checksum: e74c67a5a953194e0036f4f57bfc691d (MD5) / Made available in DSpace on 2016-04-24T02:26:52Z (GMT). No. of bitstreams: 1 rosaurarodriguestoledo.pdf: 1086955 bytes, checksum: e74c67a5a953194e0036f4f57bfc691d (MD5) Previous issue date: 2013-11-05 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / No presente trabalho o objetivo central é refletir as práticas de ressocialização do egresso do sistema prisional, seus usos e significados, com base nas interações sociais desenvolvidas no contexto das políticas públicas de atendimento ao preso e ao egresso. Neste sentido, vai investigar quais os atores imediatamente envolvidos nas políticas de ressocialização e quais os impactos destas políticas na subjetividade destes indivíduos, e consequentemente na sociabilidade, através da interpretação das observações e das percepções que emergem do contexto interacional. E, diante desta análise é realizada uma observação crítica das representações sociais envolvidas neste contexto, com vistas a refletir sobre o funcionamento das políticas criminais e o atingimento dos propósitos públicos de ressocialização. / In this paper the main objective is to reflect the rehabilitation practices to egress from the prison system, its uses and meanings, based on social interactions developed in the context of public policy for treatment of prisoner and egress. In this sense, we will investigate which actors immediately involved in the policies of rehabilitation and which the impacts of these policies on the subjectivity of these individuals, and consequently in sociability, through interpretation of observations and insights that emerge from the interaction context. And, by this analysis is performed a critical remark of social representations involved in this context, in order to reflect on the functioning of the criminal policies and the achievement of public purposes of rehabilitation.

O sistema de pós-graduação trouxe contribuições para as universidades, principalmente no âmbito da melhoria da qualidade do ensino e da produção de conhecimento. Implantada na década de 60, com o propósito de qualificar profissionais, maioria docentes da própria universidade, visava a ampliar sua formação para a pesquisa e, consequentemente, incentivar a produção de conhecimento. Neste movimento, docência e pesquisa foram fortalecidos. Ao final da década de 90, a CAPES reconhece o Mestrado Profissional - stricto senso - como modalidade de pós-graduação. Com foco nos profissionais atuantes no mercado de trabalho, propõe sua aproximação com a pesquisa, com o compromisso de alcançar e modificar o ambiente de trabalho. Paralelamente, em 1990, é fundado o Hemocentro do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Com proposta inicial de assistência e atendimento ao público, este centro de saúde conviveu com a pesquisa realizada na academia, possibilitando conciliar a produção de conhecimento com a prática desenvolvida em sua rotina. Em 2012, inaugura o mestrado profissional em Hemoterapia e Biotecnologia, com o objetivo de formar e qualificar profissionais desta área. Neste trabalho, objetivou-se estudar este programa, com a proposta de investigar os mecanismos de interação estabelecidos entre o centro de pesquisa e as unidades de trabalho parceiras, a partir da perspectiva dos egressos (1ª e 2ª turmas). Por ter um caráter exploratório e inicial, buscou-se trabalhar com indicadores exclusivos da modalidade profissional, permitindo fortalecer o acompanhamento e avaliação do programa oferecido, principalmente àqueles relacionados à aplicabilidade dos projetos e o impacto do curso realizado para a atuação profissional do egresso. Dessa forma, o presente trabalho é um estudo exploratório-descritivo com abordagens quantitativas e qualitativas, com seis tópicos principais de investigação: o programa oferecido; os projetos de pesquisa realizados; as produções intelectuais; os desdobramentos da convivência em grupo de profissionais de diferentes estados brasileiros; as contribuições do orientador para o desenvolvimento dos projetos de pesquisa e as contribuições decorrentes da aproximação dos alunos com a pesquisa. Com característica de formação específica e direcionada, com possibilidade de reunir profissionais da área da Hemoterapia de nove regiões do Brasil, o programa tem permitido alcançar objetivos previstos na modalidade profissional (stricto sensu) e almejados pelo projeto de criação de 2011. Parte destes resultados deve-se principalmente à três mecanismos de interação identificados: o projeto de pesquisa, a convivência em grupo de profissionais e o orientador, cada um com contribuições específicas para o processo de formação e aprendizagem dos alunos, permitindo alcançar mudanças nas unidades de trabalho e nas condutas e competências profissionais. Essa primeira caracterização da proposta permite criar um ponto de comparação para o programa recém implantando e que passará por sua primeira avaliação quadrienal em 2017. Dificuldades e limitações também foram diagnosticadas e necessitam ser revistas, reestruturadas e trabalhadas, como o conteúdo prático do curso, o quadro de profissionais técnico-administrativos e o tempo de titulação, com média de 35,4 meses, que exige reflexão. O acompanhamento regular dos egressos e a criação de uma plataforma contínua de construção de dados, permitirá intervir e ajustar o programa, possibilitando perceber fases de amadurecimento e crescimento, com especial atenção aos indicadores relacionados à modalidade profissional. / The postgraduate system brought contributions to universities, mainly for the improvement in the quality of education and knowledge production. Established in the decade of 1960s, with the purpose of qualifying professionals, mostly professors of the university itself, it aimed at expanding their training in research and, as a result, encouraging knowledge production. In this sense, teaching and research have been strengthened. By the end of the decade of 1990, CAPES recognized the Professional Master - stricto sensu - as a postgraduate modality. Focusing on the professionals in business, it proposes an approach to research, with the commitment of reaching and modifying work environment. In parallel, in 1990, the Blood Center of the Clinical Hospital of the Ribeirão Preto Medical School of the University of São Paulo was founded. With the initial proposal of providing assistance and care to the population, this health center has lived with the research carried out in the academy, what enabled to reconcile knowledge production with the practice developed in its daily routine. In 2012, it launched the professional master in Hemotherapy and Biotechnology, with the aim of training and qualifying professionals in this field. In this work, the objective was to study this program, proposing to investigate the mechanisms of interaction established between the research center and partner work units, from the perspective of egress students (1st and 2nd classes). Because it has an exploratory and initial character, we planned to work with indicators exclusive to the professional modality, allowing to strengthen the follow-up and evaluation of the program offered, mainly those indicators related to the applicability of projects and to the impact of the course done on the professional act of the egress student. Therefore, the present work is an exploratory-descriptive study using quantitative and qualitative approaches, with six major investigation topics: the program offered; the research project developed; intellectual production; the unfolding of the experience of groups of professionals from different states of Brazil; the contribution of the supervisor for the development of research projects; and the contribution resulting from the approach of students to research. With the features of specific training and possibility of gathering professionals of the area of Hemotherapy from the nine regions of Brazil, the program has enabled to accomplish the objectives expected in the professional modality (stricto sensu) and established in the project creation in 2011. Part of these results is owed primarily to three mechanisms of interaction identified: the research project, experience of living as a group, and supervisor, each one with specific contributions to the process of students\' training and learning, enabling to achieve changes in work units and in professional conduct and competences. This first characterization of the proposal allows to create a comparison point for the recently implemented program, which will go through its first four-yearly assessment in 2017. Difficulties and limitations have also been diagnosed and must to be reviewed, restructured and managed, such as the practical content of the course, the chart of technical administrative professionals and the time for titling, with an average of 35.4 months, what demands some thoughts. The regular follow-up of egress students and creation of a continuous platform for data construction will allow to intervene and adjust the program, making possible to notice maturation and growth phases, with special attention to indicators related to the professional modality.

Made available in DSpace on 2016-01-26T18:49:38Z (GMT). No. of bitstreams: 1 FRANCISCO_DISSERTACAO_final Francisco.pdf: 1896886 bytes, checksum: af5492dff9b4dfd6836faec9c6072fc4 (MD5) Previous issue date: 2011-02-24 / This work is the result of a research developed in the Master Program of Education, search line 2 Pedagogical Formation and Practice of Teacher from Universidade do Oeste Paulista - UNOESTE. The research aimed to investigate how the graduated students from Tourism Course with emphasis in hotel, realized the role of their Course in their professional lives. The quantity-qualitative research involved 48 graduated students. The data were collected from a questionnaire with opened and closed questions. Concerning to the results, the search brought important information to a reflexive auto-evaluation process as: while students, the majority of them had expectations about the insertion of this professional in the tourism area, and nowadays, half of them are out of their professional area; only 33,3% declared absolutely satisfied with the course; in suggesting changes to improve the Course, the focus was in practical activities, apparently trying to translate a technician view of the Pedagogical Politic Project of the Course. Concluding, the dialogue with the graduated students was essential to renew the thought of the Course staff bringing challenge questions. / Esta dissertação é resultado de pesquisa desenvolvida no Programa de Mestrado em Educação, linha de pesquisa 2 Formação e Prática Pedagógica do Profissional Docente, da Universidade do Oeste Paulista. A pesquisa teve como objetivo investigar a forma como os egressos do Curso de Turismo da Unoeste percebem o papel que o curso desempenhou na sua formação profissional. A pesquisa caracterizou-se como quanti-qualitativa, envolvendo 48 egressos do curso de Turismo, com ênfase em Hotelaria, da Unoeste. Para a coleta de dados recorreu-se a um questionário com perguntas fechadas e com perguntas abertas. Em relação aos resultados, a pesquisa trouxe importantes informações para o processo reflexivo de autoavaliação, por exemplo: enquanto eram alunos, a maioria nutria expectativa da inserção do profissional na área de turismo; hoje, como egressos, a metade está fora da área do bacharelado; apenas 33,3% dos egressos manifestaram-se como plenamente satisfeitos com o curso. Ao sugerirem mudanças para melhorar o curso, o foco foi em atividades práticas, parecendo traduzir uma visão tecnicista do Projeto Político Pedagógico do curso. Concluindo, o diálogo com os egressos é fundamental para a renovação das linhas de pensamento do curso, trazendo perguntas desafiadoras.