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O gás composto monóxido de carbono (CO), está envolvido na modulação de diferentes funções orgânicas, tais como a regulação cardiovascular, a temperatura corporal e a nocicepção. A participação do CO nos processos fisiológicos ocorre por meio da atividade da enzima heme-oxigenase (HO), e seu produto CO, o qual por sua vez aumenta a produção de guanosina monofosfato ciclíco intracelular (GMPc). De particular interesse, o locus coeruleus possui elevada expressão da enzima HO-2 sugerindo o envolvimento do gasotransmissor CO na modulação das funções executadas por esta estrutura encefálica. O objetivo deste trabalho foi avaliar o envolvimento da via HO-CO do LC na modulação da ansiedade, avaliada pelo teste de labirinto em cruz elevado e teste claro-escuro; nocicepção aguda, avaliada pelo teste de retirada de cauda e a nocicepção inflamatória, avaliada pelo teste de formalina em ratos. Para atingir estes objetivos, ratos (± 250grs; Wistar) foram anestesiados (ketamina 75 mg/kg e xilasina 10 mg/kg i.m.) e submetidos à cirurgia estereotáxica para implante unilateral de cânulas-guias direcionadas para o LC, e para o ventrículo lateral. Após o período de recuperação, os ratos foram divididos em distintos grupos experimentais para administração intra-LC do ZnDPBG (inibidor inespecífico da enzima HO, nas doses 5,50 ou 200 nmol/0,1 µl) ou seu veículo, Na2CO3 (50 mmol/0,1 µl); do Heme-lisinato (150, 300 ou 600 nmol/0,1 µl) ou seu veículo, L-lisina (14,2 µmol/0,1 µl); do ODQ i.c.v. (inibidor específico da enzima guanilase ciclase solúvel, 1,3 nmol/1,0 µl) ou seu veículo (DMSO 1%, 1,0 µl) e após 15 min o Heme-lisinato (600 nmol/0,1 µl) ou seu veículo (L-lisina, 14,2 µmol/0,1 µl), intra-LC. Após o tempo de 15 min, os ratos foram avaliados no teste de LCE ou no TCE por 5 minutos, no teste de retirada de cauda por 120 minutos e no teste de formalina intra-podal por 45 minutos. Os resultados obtidos mostram que o aumento da produção do neuromodulador gasoso CO no LC, pela ativação da via HO-CO-GMPc com Heme-lisinato, promove efeito ansiolítico avaliado no teste do LCE e no TCE, evidenciado pelo aumento do tempo de permanência e pelo aumento do número de entradas nos braços abertos do LCE, e pelo aumento tempo de permanência no compartimento claro do TCE. Este efeito ansiolítico é dependente da atividade de GMPc intracelular, desde que o tratamento i.c.v. com inibidor específico da enzima GCs bloqueou os efeitos do Heme-lisinato no LCE e no TCE. Ainda, a ativação da via HO-CO-GMPc por meio da administração intra-LC do Heme-lisinato promoveu efeito antinociceptivo frente estímulo térmico agudo (teste de retirada de cauda em ratos), sendo este efeito dependente da atividade do GMPc, desde que o pré-tratamento com o inibidor da enzima guanilase ciclase solúvel, ODQ, bloqueou o aumento do IARC. O bloqueio da via HO-CO promove efeito hipernociceptivo em modelo de dor inflamatória, desde que o tratamento intra-LC com inibidor inespecífico da HO, ZnDPBG aumenta o número de sacudidas no teste de formalina intra-podal. Assim, este estudo é pioneiro em demonstrar que o neuromodulador CO do LC modula a ansiedade e a nocicepção aguda térmica e inflamatória. / The gas composed carbon monoxide (CO) is involved in the modulation of various physiological functions such as cardiovascular regulation, nociception and body temperature. CO participation in physiological processes occurs through the activity of the enzyme heme oxygenase (HO), and its product CO, which in turn increases the production of intracellular cyclic guanosine monophosphate (cGMP). In particular interest, the locus coeruleus (LC) has a high HO-2 enzyme expression suggesting the involvement of CO in the modulation of the functions performed by this brain structure. The aim of this study was to evaluate the involvement of HO-CO pathway of LC in modulating anxiety, assessed by elevated plus maze test and light-dark box test. Additionally, acute nociception, as assessed by the tail flick test and inflammatory nociception, as assessed by formalin test in rats were analyzed after HO-CO pathway activation. Rats (±250 grs; Wistar) were anesthetized (ketamine 75 mg/kg and xylazine 10 mg/kg im) and underwent stereotactic surgery for cannulas guides unilateral implantation directed to the LC, and to the lateral ventricle. After the recovery period, rats were divided into distinct experimental groups for intra-LC ZnDPBG (nonspecific enzyme inhibitor HO doses 5, 50 or 200 nmol/0.l µl) or its vehicle, Na2CO3 (50 mmol/0.l µl); Heme-lysinate (150, 300 or 600 nmol/0.l µl) or its vehicle, L-lysine (14.2 nmol/0.1 µl), the ODQ i.c.v. (specific inhibitor of the enzyme guanilase soluble cyclase, 1.3 nmol/1.0 µl) or its vehicle (1% DMSO, 1.0 µl) and after 15 min the Heme-lysinate (600 nmol/0.1 µl), or its vehicle (L-lysine, 14.2 mmol/0.1 µl), intra-LC. After time 15 min, rats were evaluated in the EPM test or LDB for 5 minutes and in the tail flick test for 120 minutes and in the formalin test for 45 minutes. The results show that CO increased production in LC, by HO-CO-cGMP pathway activation, promotes anxiolytic effect evaluated in the EPM test and LDB. The anxiolytic effect is dependent on the activity of intracellular cGMP, since treatment i.c.v. with enzyme sGC inhibitor blocked the effects of Heme-lysinate. Moreover, the activation of the HO-CO-cGMP pathway into the LC promoted antinociceptive effect in the tail flick test, this effect being dependent on the activity of cGMP, since pre-treatment with the guanilase cyclase soluble inhibitor, ODQ, blocked the increase in analgesic index. Furthermore, the block of the HO-CO pathway intra-LC promoted hypernociception in a model of inflammatory pain, since treatment with nonspecific inhibitor HO, ZnDPBG, increases the nociceptive behavior in the formalin test. Thus, this study is the first to demonstrate that the CO neuromodulator into LC modulates anxiety and acute thermal and inflammatory nociception.

O gás composto monóxido de carbono (CO), está envolvido na modulação de diferentes funções orgânicas, tais como a regulação cardiovascular, a temperatura corporal e a nocicepção. A participação do CO nos processos fisiológicos ocorre por meio da atividade da enzima heme-oxigenase (HO), e seu produto CO, o qual por sua vez aumenta a produção de guanosina monofosfato ciclíco intracelular (GMPc). De particular interesse, o locus coeruleus possui elevada expressão da enzima HO-2 sugerindo o envolvimento do gasotransmissor CO na modulação das funções executadas por esta estrutura encefálica. O objetivo deste trabalho foi avaliar o envolvimento da via HO-CO do LC na modulação da ansiedade, avaliada pelo teste de labirinto em cruz elevado e teste claro-escuro; nocicepção aguda, avaliada pelo teste de retirada de cauda e a nocicepção inflamatória, avaliada pelo teste de formalina em ratos. Para atingir estes objetivos, ratos (± 250grs; Wistar) foram anestesiados (ketamina 75 mg/kg e xilasina 10 mg/kg i.m.) e submetidos à cirurgia estereotáxica para implante unilateral de cânulas-guias direcionadas para o LC, e para o ventrículo lateral. Após o período de recuperação, os ratos foram divididos em distintos grupos experimentais para administração intra-LC do ZnDPBG (inibidor inespecífico da enzima HO, nas doses 5,50 ou 200 nmol/0,1 µl) ou seu veículo, Na2CO3 (50 mmol/0,1 µl); do Heme-lisinato (150, 300 ou 600 nmol/0,1 µl) ou seu veículo, L-lisina (14,2 µmol/0,1 µl); do ODQ i.c.v. (inibidor específico da enzima guanilase ciclase solúvel, 1,3 nmol/1,0 µl) ou seu veículo (DMSO 1%, 1,0 µl) e após 15 min o Heme-lisinato (600 nmol/0,1 µl) ou seu veículo (L-lisina, 14,2 µmol/0,1 µl), intra-LC. Após o tempo de 15 min, os ratos foram avaliados no teste de LCE ou no TCE por 5 minutos, no teste de retirada de cauda por 120 minutos e no teste de formalina intra-podal por 45 minutos. Os resultados obtidos mostram que o aumento da produção do neuromodulador gasoso CO no LC, pela ativação da via HO-CO-GMPc com Heme-lisinato, promove efeito ansiolítico avaliado no teste do LCE e no TCE, evidenciado pelo aumento do tempo de permanência e pelo aumento do número de entradas nos braços abertos do LCE, e pelo aumento tempo de permanência no compartimento claro do TCE. Este efeito ansiolítico é dependente da atividade de GMPc intracelular, desde que o tratamento i.c.v. com inibidor específico da enzima GCs bloqueou os efeitos do Heme-lisinato no LCE e no TCE. Ainda, a ativação da via HO-CO-GMPc por meio da administração intra-LC do Heme-lisinato promoveu efeito antinociceptivo frente estímulo térmico agudo (teste de retirada de cauda em ratos), sendo este efeito dependente da atividade do GMPc, desde que o pré-tratamento com o inibidor da enzima guanilase ciclase solúvel, ODQ, bloqueou o aumento do IARC. O bloqueio da via HO-CO promove efeito hipernociceptivo em modelo de dor inflamatória, desde que o tratamento intra-LC com inibidor inespecífico da HO, ZnDPBG aumenta o número de sacudidas no teste de formalina intra-podal. Assim, este estudo é pioneiro em demonstrar que o neuromodulador CO do LC modula a ansiedade e a nocicepção aguda térmica e inflamatória. / The gas composed carbon monoxide (CO) is involved in the modulation of various physiological functions such as cardiovascular regulation, nociception and body temperature. CO participation in physiological processes occurs through the activity of the enzyme heme oxygenase (HO), and its product CO, which in turn increases the production of intracellular cyclic guanosine monophosphate (cGMP). In particular interest, the locus coeruleus (LC) has a high HO-2 enzyme expression suggesting the involvement of CO in the modulation of the functions performed by this brain structure. The aim of this study was to evaluate the involvement of HO-CO pathway of LC in modulating anxiety, assessed by elevated plus maze test and light-dark box test. Additionally, acute nociception, as assessed by the tail flick test and inflammatory nociception, as assessed by formalin test in rats were analyzed after HO-CO pathway activation. Rats (±250 grs; Wistar) were anesthetized (ketamine 75 mg/kg and xylazine 10 mg/kg im) and underwent stereotactic surgery for cannulas guides unilateral implantation directed to the LC, and to the lateral ventricle. After the recovery period, rats were divided into distinct experimental groups for intra-LC ZnDPBG (nonspecific enzyme inhibitor HO doses 5, 50 or 200 nmol/0.l µl) or its vehicle, Na2CO3 (50 mmol/0.l µl); Heme-lysinate (150, 300 or 600 nmol/0.l µl) or its vehicle, L-lysine (14.2 nmol/0.1 µl), the ODQ i.c.v. (specific inhibitor of the enzyme guanilase soluble cyclase, 1.3 nmol/1.0 µl) or its vehicle (1% DMSO, 1.0 µl) and after 15 min the Heme-lysinate (600 nmol/0.1 µl), or its vehicle (L-lysine, 14.2 mmol/0.1 µl), intra-LC. After time 15 min, rats were evaluated in the EPM test or LDB for 5 minutes and in the tail flick test for 120 minutes and in the formalin test for 45 minutes. The results show that CO increased production in LC, by HO-CO-cGMP pathway activation, promotes anxiolytic effect evaluated in the EPM test and LDB. The anxiolytic effect is dependent on the activity of intracellular cGMP, since treatment i.c.v. with enzyme sGC inhibitor blocked the effects of Heme-lysinate. Moreover, the activation of the HO-CO-cGMP pathway into the LC promoted antinociceptive effect in the tail flick test, this effect being dependent on the activity of cGMP, since pre-treatment with the guanilase cyclase soluble inhibitor, ODQ, blocked the increase in analgesic index. Furthermore, the block of the HO-CO pathway intra-LC promoted hypernociception in a model of inflammatory pain, since treatment with nonspecific inhibitor HO, ZnDPBG, increases the nociceptive behavior in the formalin test. Thus, this study is the first to demonstrate that the CO neuromodulator into LC modulates anxiety and acute thermal and inflammatory nociception.

Students with disabilities, some with emotional and behavior disorders, are included in almost all elementary music classes. Students with emotional behavior disorders are one of the greatest challenges for teachers. To be effective, teachers must develop strategies and inclusive practices specifically geared towards intervention. With the quantity of students served and only limited class time with students, the music teacher is often unaware of the unique needs of specific special learners. Music teacher preparation has been inadequate in training teachers for inclusion. Elementary music educators rarely have outside support to deal with classroom challenges as they serve students with disabilities and at-risk students. Music teacher training is focused on content, not behavior management. This study examined the perceptions of randomly selected elementary music educators who were members of MENC: The National Association for Music Education regarding their preparedness to effectively manage five areas of severe behavior often exhibited by students with emotional behavior disorders: withdrawal, impulsivity, argumentative behavior, aggression towards peers, and aggression towards the teacher. The researcher devised a 39 item online survey instrument based on supporting literature. The survey was given to randomly selected participants. Two hundred sixty-nine elementary music educators from across the United States completed the survey providing information on incidence frequency, preparedness, training in behavior management, and the amount of behavior support available. Elementary music teachers felt prepared to handle impulsivity (58.2%), and argumentative behavior (55.7%). They were not prepared for withdrawal (50.8%), aggression towards peers (50.9%), and least prepared to handle aggression towards the teacher (58.1%).Over 94% of the music teachers had adult assistance less than 25% of the time and 45.9% never had adult assistance with included classes. More than 74% of the teachers indicated that they have adult assistance with self-contained special education classes less than 25% of the time and 35.7% never having adult assistance with those classes. Forty-six point two percent of the music teachers had no behavior specialist available or were unaware if one was available. Only 3.7% of the respondents felt they had all the support they needed. Thirty-six point one percent of the music teachers had no crisis plan in case of an eruption of severe behavior in their classes.

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Made available in DSpace on 2016-07-27T14:22:20Z (GMT). No. of bitstreams: 1 Roberta Maia Marcon.pdf: 7289173 bytes, checksum: 9740dc6034b1d226414db7176cb724e4 (MD5) Previous issue date: 2010-03-15 / This study aimed at functionally analyzing the verbal behavior of a person diagnosed with schizophrenia undergoing treatment in a specialized service. The participant is a female, diagnosed with simple schizophrenia, 27 years old, from the state of Bahia, single, incomplete elementary school, low social-economic status, and presenting a history of treatment in several institutions. To control the procedures, we used the design of multiple conditions with three main conditions: condition attention (A), condition alone (S), and condition control (C). Condition (A) was manipulated in four sub-conditions: (A1) attention, eye contact (inappropriate speech was followed by 10 seconds of eye contact); (A2) attention, physical contact (inappropriate speech was followed by 10 seconds of physical contact); (A3) attention, comment (inappropriate speech was followed by the comment It is difficult to understand when you talk like this ); and (A4) attention, task execution (appropriate speech was followed by social attention in the form of signs of approval); condition (S) was manipulated in two sub-conditions: (S1) alone, without demand (participant remained in the room in the absence of the researcher) and (S2) alone, with demand (participant remained in the room in the absence of the researcher, with demand); and condition (C) (participant remained in the room composed by reinforcers, in the presence of the researcher, who was writing on a sheet of paper). Under the conditions studied, we also aimed at observing some emotional behaviors of the participant, such as annoyance, anger, pleasure, elation, anxiety, sadness, and relief inferred from the intonation of her voice and her behavioral topography. The results demonstrated that the social attention manipulated under the different conditions controlled the participant s inappropriate speech, which may have worked as a motivational operation for the occurrence of her verbalization. They also demonstrated that under condition (A) there were more manifestations of emotional phenomena, and rage (attention, eye contact, and alone, without demands) was the most frequent emotion, followed by elation (attention, comment), whereas the least frequent emotions were pleasure and relief (attention, comment, and eye contact, respectively). These findings are discussed in terms of the implications of the effects of a motivational operation as a component for the functional analysis of the verbal behavior of the participant in the present investigation. / O presente estudo objetivou analisar funcionalmente o comportamento verbal de uma pessoa esquizofrênica, que se encontrava em tratamento especializado em um Centro de Atenção Psicossocial (CAPS), fazendo uso de uma metodologia de análise funcional. A participante era do sexo feminino, diagnosticada com esquizofrenia simples, 27 anos de idade à época, natural do estado da Bahia, solteira, primeiro grau incompleto, de nível socioeconômico baixo e com histórico de internação em várias instituições. Para essa finalidade foi empregado o delineamento de múltiplas condições com três condições principais: condição atenção (A), condição sozinha (S) e condição controle (C). A condição (A) incluiu quatro subcondições: (A1) atenção, contato olho a olho (a fala inapropriada foi seguida por 10 segundos de contato olho a olho); (A2) atenção, contato físico (a fala inapropriada foi seguida por 10 segundos de contato físico); (A3) atenção, comentário (a fala inapropriada foi seguida pelo comentário Fica difícil compreender quando você fala assim ); e (A4) atenção, executar tarefa (o comportamento apropriado foi seguido pela atenção social sob a forma de sinais de aprovação); a condição (S) incluiu duas subcondições: (S1) sozinha, sem demanda (a participante permaneceu na sala na ausência da pesquisadora) e (S2) sozinha, com demanda (a participante permaneceu na sala na ausência da pesquisadora, com demanda); e a condição (C) (a participante permaneceu na sala composta por reforçadores, na presença da pesquisadora, que escrevia em uma folha de papel). Dentro das condições estudadas foi também objeto deste estudo observar alguns comportamentos emocionais da participante, tais como aborrecimento, raiva, prazer, elação, ansiedade, tristeza e alívio inferidos das entonações de sua voz e de suas topografias comportamentais. Os resultados demonstraram que a atenção social manipulada nas diferentes condições exerceu controle sobre o comportamento verbal da participante, o que pode ter funcionado como uma operação motivadora para a ocorrência de suas verbalizações. Demonstraram, ainda, que na condição atenção (A) houve maiores manifestações dos comportamentos emocionais, sendo a raiva a emoção mais frequente, seguida pela elação, enquanto as de menores ocorrências foram prazer e alívio. Esses achados foram discutidos em termos das implicações dos efeitos de uma operação motivadora como componente para a análise funcional do comportamento verbal da participante da presente investigação.

La grande diversité des réponses de stress observée entre individus a pour origine des facteurs génétiques en interaction avec des facteurs environnementaux. Certaines réponses peuvent être moins adaptées et accroitre la vulnérabilité de l’individu aux divers troubles et pathologies liées au stress. La CBG est une glycoprotéine plasmatique impliquée dans la biodisponibilité des glucocorticoïdes, un des principaux médiateurs de la réponse au stress. Des études génétiques ont montré que des polymorphismes du gène codant la CBG ont un impact significatif sur la réponse des glucocorticoïdes au stress. Pour comprendre les mécanismes de l’impact de la CBG sur l’action des glucocorticoïdes et les conséquences sur les réponses endocriniennes et comportementales de stress, notre équipe a développé un modèle de souris déficiente pour le gène Cbg. Ces souris présentent une réponse diminuée des glucocorticoïdes au stress, associée à un niveau élevé de comportement émotionnel de type dépressif. Cette thèse a pour but d’explorer plus en profondeur les altérations physiologiques et comportementales des souris Cbg ko. Nous avons montré que le niveau plus faible de glucocorticoïdes observé chez la souris Cbg ko provient d’une élimination plasmatique plus importante. Une étude chez la souris Cbg ko femelles a montré que les estrogènes se surimposent à la déficience en CBG pour induire des comportements de type dépressif. Nous avons également démontré que la déficience en CBG conduit a une atténuation de la sensibilité comportementale et endocrinienne au stress chronique. Enfin, nous avons observé une détérioration de la mémoire long terme de ces souris. Par ailleurs, nous montrons que dans des conditions de stress chronique associé à un régime alimentaire déséquilibré le métabolisme du glucose était altéré chez les animaux déficients en CBG. Ces résultats renforcent l’importance du rôle de la CBG influençant l’ensemble des mécanismes d’actions des glucocorticoïdes par la modulation de leurs niveaux et de leur disponibilité. / The great diversity in the response to stress observed among individuals originates from their genetic background in interaction with environmental factors. Some responses can be less adaptive and increase the vulnerability to develop stress-associated disorders. CBG is a plasma glycoprotein that regulates the bioavailability of glucocorticoids, one of the main mediators of the stress response. Genetic studies pointed out variations in the gene coding for CBG as a major factor influencing the glucocorticoid response to stress. To better understand the mechanisms involved and the consequences on endocrine and behavioral responses to stress, our team has developed a mouse model of CBG deficiency. These mice present blunted glucocorticoid response to stress associated with increased despair-like behaviors. This thesis aimed at further exploring the physiological and behavioral alterations presented by the Cbg ko mice. We showed that the lower glucocorticoid levels observed in Cbg ko mice stems from their higher clearance from plasma. A study performed on Cbg ko female mice revealed that estrogens outpass CBG deficiency in inducing despair-like behavior. Additionally, we evidenced that CBG deficiency leads to lower behavioral and endocrine sensitivity to chronic stress, and we observed impairment of hippocampal-dependent long-term memory in these mice. Finally, we found that chronic stress combined to high-fat diet leads to alteration in glucose metabolism in CBG deficient animals. These findings reinforce the important role of CBG influencing the broad range of actions of glucocorticoids by modulating their levels and availability.

Abstract Adverse Childhood Experiences and Trauma-Informed Schools: Restorative Practices for Social and Emotional Behavior Issues in Education by Heather Easterling The purpose of this study was to determine whether factors in school climate and culture and the educator's role in evaluating adverse childhood experiences (ACEs), trauma-informed situations, and disruptive classroom behaviors, enable a school to generate interventions needed to help students succeed. This qualitative study evaluated the ACEs and the relationships traumatic incidents had on disruptive classroom behaviors that teachers experienced with students. Although there is emerging research regarding ACEs and trauma-informed schools, there are multiple factors that facilitate the relationship between ACEs and disruptive classroom experiences. Data collection strategies included the use of interview procedures and document review. This evidence was gathered from teachers who provided their experiences with disruptive classroom behaviors and their experiences with behavior intervention programs such as RTI2B (Response to Instruction and Intervention for Behavior) and trauma-informed practices. Analysis of data occurred in three phases: (a) categorization based on emergent themes from the interviews, (b) constructing the explanation in narrative form, and (c) re-examination of the collected data concerning discipline referrals, school climate surveys, and attendance surveys. This research study provided insight into experiences teachers had with disruptive classroom behaviors. The experiences showed implementation of trauma-informed practices, a positive behavior program, and the need for a support system for teachers to be able to better reach students who have experienced ACEs that are related to disruptive classroom behaviors. The results revealed that there were direct factors that determined that the relationship between ACEs and trauma were consistent with disruptive classroom behaviors.

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No description available.