Change Management (CM) plays a key role to manage an individual, a group, or an organization from a current state to a desired future state. The purpose of CM is to promote and support activities and actions to higher productivity levels and higher adoption rates simultaneously as stakeholder’s commitment, comfort, and confidence increase in e.g. new solutions, services, products, systems, tools, methods, or ways of workings Previous research claims a gap between CM theory and practice exists, and that a vast number of theories and models available to academics and practitioners is both contradictory and confusing, and that limited empirical evidence supports of the plethora of CM frameworks and models. Thus, this study investigates and clarifies CM-related activities and factors, that could contribute to bridge the gap between practice and research. Regarding the research process, this study builds upon a literature review and a case study. Additionally, a conceptual CM model is composed which builds upon CM-related aspects, activities, enablers, and factors. The case study focuses on lessons learned and current CM practices. In the latter parts of study is the conceptual CM model applied to the empirical findings to find and analyze differences and gaps between practice and theory. At last, is it concluded that no single CM model alone, covers the plethora of activities and factors that are listed in the conceptual CM model. Hence, this study recommends both practitioners and researchers to take multiple CM models into consideration, to support and potentially improve CM practices. In conclusion, this study confirm that a research gap exists between practice and research, as several significant and distinguishing CM-related activities and factors, have been identified and analyzed. / Change Management (CM) utgör en essentiell managementdisciplin som fokuserar på att möjliggöra en effektiv styrning och hantering och individer, grupper och organisationer genom att facilitera aktiviteter för att uppnå en förbättrad och högre nivå av förändringsbenägenhet, organisatorisk produktivitet, samtidigt som både externa och interna intressenters engagemang, delaktighet, tillit och adoption av nya lösningar, tjänster, produkter, system, verktyg, metoder eller arbetssätt ökar eller förbättras. I tidigare forskning påstås det finnas ett gap mellan teori och praxis-fältet. Parallellt, påyrkas det även att ett stort antal teorier och modeller som akademiker, experter, tjänstemän, lekmän ofta tillämpar eller relaterar till kan vara både motsägelsefulla och osammanhängande. Därutöver, anses det även att begränsade och bristfälliga empiriska bevis tillhandahållits nutilldags för att stödja den mångfald av CM-relaterade modeller, ramverk och tillvägagångssätt. Således, är syftet med denna studie att identifiera och granska CM-relaterade aktiviteter och faktorer som kan bidra till att överbrygga klyftan mellan CM-forskning och praxis. Med hänseende till forskningsprocessen, baseras denna på både en litteraturgenomgång, en konceptuell CM modell och en fallstudie som är utförd på ett företag inom IKT-branschen. Den föreslagna och konceptuella CM-modellen bygger på CM-relaterade aktiviteter och faktorer. Vidare fokuserar fallstudien på en aktuell CM strategi, samt erfarenheter och lärdomar från tidigare och avslutade projekt. Studien är dessutom avgränsad till planerade, strategiska och tvärfunktionella projekt. Yttermera, tillämpas den konceptuella modellen senare på de empiriska resultaten med avsikt understödja identifieringen och analysen av bristfälligheter och skiljaktigheter mellan teori och praxis-fältet. Avslutningsvis, dras slutsatsen att ingen CM-modell som identifierats i CM-litteraturen i avskildhet täcker mängden av aktiviteter och faktorer som anges i den föreslagna konceptuella CM-modellen. Således, rekommenderas det i denna studie att organisatoriska experter, tjänstemän och akademiker bör ta hänsyn till, samt integrera åtskilliga CM-modeller, med avsikt att främja och sålunda potentiellt förbättra handlingssätt och utföranden relaterade till CM-relaterade aktiviteter och procedurer, på ett mer allomfattande, metodiskt och effektivt vis. Därtill, bekräftas det i denna studie att det finns ett faktiskt gap mellan forskning och praxis fältet, då ett flertal avvikande och särskiljande CM-relaterade aktiviteter och faktorer har identifiserats, analyserats och diskuterats.

The Tumor Necrosis Factor derived-TIP peptide is a small 17 amino acids cyclic peptide with lectin-like activity, that possesses several therapeutically relevant biological activities, among which is activation of alveolar liquid clearance in both healthy and injured lungs in vivo. Accumulation of fluid in the lungs? alveoli and interstitial spaces is a life-threatening condition called pulmonary edema. The mortality rate due permeability pulmonary edema, accompanied by a dysfunction of the alveolar/capillary barrier, is high because no effective treatment lacking side effects exists nowadays. It is known that the TIP peptide is able to activate vectorial Na+ transport ? which mediates lung liquid clearance. However, the mechanism of action of remains elusive. The aim of this thesis was to investigate the initial steps of interaction between the TIP peptide and airway epithelial cells. Numerous novel methods and single-molecule techniques were used to unravel: (i) how the TIP peptide interacts with the molecules on the apical side of the lung epithelial cells; (ii) whether the TIP peptide need to be internalized inside of the cells to trigger its effects; (iii) the nature of the interaction between the TIP peptide and its putative receptor(s); (iv) the putative receptor(s) for the TIP peptide on the apical surface of the lung epithelial cells.

The aim of this thesis was to establish methods for selected PAPP-A (Pregnancy- Associated Plasma Protein A) gene polymorphisms analysis and to study genetic background of PAPP-A and biochemical background of PAPP-A and PlGF (Placental Growth Factor) in relation to risk pregnancy. Secondly, the aim was to establish method for two-dimensional (2D) electrophoresis of amniotic fluid. Methods for analysis of ten PAPP-A gene polymorphisms were established. These polymorphisms, PAPP-A and PlGF levels were studied in together 165 women in third trimester pregnancies complicated with threatening preterm labor (n=98), preeclampsia (n=35), IUGR (Intrauterine Growth Restriction) (n=34) and ICP (Intrahepatic Cholestasis of Pregnancy) (n=15). 114 healthy pregnant women served as controls. The method for 2D electrophoresis of amniotic fluid was established. Preeclamptic patients had significantly higher frequency of TT genotype of Cys327Cys (C/T) PAPP-A gene polymorphism compared to controls. Patients with ICP had increased serum levels of PAPP-A compared to controls, in patients with threatening preterm labor PAPP-A levels were rather decreased. PlGF levels did not differ from control group in patients with ICP and threatening preterm labor. Positive correlation was found between PAPP-A and PlGF in group of...

Criminal behaviour among youths is spread so widely, that almost every youth has committed one criminal aktivity at least. The top of the criminal activities of youth committers came in the half nineties. Since that time, there have been noticed new trends in youth{\crq}s criminality development. The average age of committers decreases, the brutality of crime increases and there came out new kinds of crime (children prostitution, drug criminality). In the last time it is more often discussed lowering the age limit of penal responsibility. It would be since 14 years. A new penal legislative could be authorized at the break of this and next year. This diploma work aims to chart the society{\crq}s attitude towards criminal activity of youths in chosen areas of south bohemian region. Individual points of this work are orientated towards the attitudes to the age limit of penal responsibility, to the current legislative reform in punishable activities of youths; to risky factors which cause arise of criminal behaviour. The first hypothesis was set up on the thought, that the risky factor which has an effect on arising the criminal behaviour of youths is preferred by the public in consistence in parents bringing up to school setting. Hypothesis I. was confirmed. Hypothesis one claimed that the laic public is not partisan of setting op cameras at schools. This hypothesis was disapproved. Hypothesis II. assumed that positive laic public{\crq}s attitude are for lowering the age limit of penal responsibility. This hypothesis was confirmed. Before the research, there had come out a hypothesis III. which assumes professionals positive attitude towards lowering the age limit of penal responsibility. There was pursued a research with some professionals work in a field of penal affairs of youths. There was made a half standardized interview. In the second part was done a quatitative research. That research was realized on the selective set which had 210 respondents. Some inhabitants of České Budějovice and Český Krumlov regions took part in this research. The main implement used in this research was a semantic diferencial. This diploma work should help to spread awareness and knowledge of the crime problematic of youths. Next usage could be used by professionals who are interestend in attitudes towards this problematic.

Lyme borreliosis is a multisystemic disease affecting skin, joints, heart and central nervous system. The disease is caused by spirochetes of Borrelia burgdorferi sensu lato complex. These bacteria are spread by ticks of Ixodes genus. In 2016 there were almost 4,000 newly infected individuals reported in the Czech Republic. Contemporary serological diagnostics of Lyme borreliosis is not sensitive nor specific enough and does not even correlate with the pathology of the disease in the early or late phases. For the correct diagnosis of the disease it is necessary to detect the pathogen and its genotype. For this reason we had aimed at two goals. Through the digital droplet PCR (ddPCR) method we detected Borrelia-specific DNA and its genotype. The detection limit of borrelial DNA was set on gDNA samples isolated from the tick. Detection threshold for the initial amount of 1 ng of tick gDNA is at the range of 10-17 g of specific borrelial DNA. Borrelia spp. coinfection was detected in 5 out of 12 tested samples. The most frequent type was B. garinii which was detected in 5 samples. On the basis of published sequences for virulent factors we have designed specific primers in conserved regions of the genes flanking their variable segments to be PCR amplified. Gene variability will be monitored through...

Diploma thesis covers the topic about different attitudes to DCF valuation. The first part is an introduction into CAPM theory and a multifactor French-Fama model. This part also indicates different views on financial assets and analyzes an issue of setting discount rates, especially the risk-free rate and equity risk premium. The second part of this paper applies the theory into valuation of Microsoft, GAP and Telefónica O2. There are elaborated forecasts of the financial statements and free cash flows (FCFCE, FCFU), the discount rate composition and analyses of the factors HML and SMB. At the end, there are performed various valuations, which results are discussed together with a development of real market prices.

Costa Rica’s annual mean precipitation is above 3300 mm, but this precipitation is not evenly distributed in time or space, producing clear differentiated wet and dry seasons in most of the country. Droughts are also common phenomena which greatly affect the availability of water resources. Managed aquifer recharge (MAR) schemes are being taken into consideration to enhance the underground water storage capacity of the country. The present study constitutes the first assessment for the identification of suitable sites for the implementation of MAR technology spreading methods (SM) in Costa Rica. The suitable sites are identified by means of a geographic information system multi-criteria decision analysis (GIS-MCDA) based on four criteria: hydrogeological aptitude, terrain slope, top soil texture and drainage network density. Four steps are performed in order to identify these sites: problem definition, screening for suitable areas, suitability mapping, and sensitivity analysis. The suitability map was divided in two zones after the screening: suitable and unsuitable, the first zone was further divided in five classes according to the weighted linear combination (WLC) ranking. The results indicate that 61% of the country is suitable for conducting SM. This map is a tool for future implementation of MAR techniques in the country.

This diploma thesis deals with the different possible calculations of crack path. Specifically, it focuses on criteria based on maximum tangential stress, minimal strain energy density, crack tip displacement, and local symmetry. These criteria are used for calculations in ANSYS software to estimate possible crack paths on four simple structures. The thesis also contains the codes created in ANSYS. Using these, the crack trajectory of a given structure can be calculated by any of the four criteria described.

Předkládaná dizertační práce se zabývá stanovením hlavních členů Williamsova asymptotického rozvoje popisujícího rovinné elektro-elastické pole v okolí piezoelektrických bi-materiálových vrubů a trhlin na rozhraní za použití rozšířeného Lechnického-Eshelbyho-Strohova formalismu v návaznosti na čistě anizotropní pružnost. Je ukázáno, že rozšířený Lechnického-Eshelbyho-Strohův formalismus představuje spolu s moderními programovacími koncepty v jazyku Python efektivní a také praktický nástroj pro lomovou analýzu piezoelektrických bi-materiálů. Teoretická část práce popisuje aspekty anizotropní pružnosti a její návaznost na piezoelektrické materiály. Základní rovnice zaměřené na speciální typy monoklinických materiálů, které umožňují oddělení rovinného a anti-rovinného problému, jsou vyjádřeny pomocí komplexních potenciálů. V praktické části práce je sestaven problém vlastního hodnot pro bi-materiálový vrub, na jehož základě jsou stanoveny exponenty singularity a pomocí dvoustavového -integrálu také zobecněné faktory intenzity napětí. Veškeré vztahy a numerické procedury jsou následně rozšířeny na problém piezoelektrických bi-materiálových vrubů a podrobně prozkoumány v uvedených příkladech. Zvláštní pozornost je věnována přechodu asymptotického řešení téměř zavřených vrubů a trhlin na rozhraní. Vliv směru polarizace na asymptotické řešení je také zkoumán. Přesnost stanovení zobecněných faktorů intenzity napětí je testována srovnáním asymptotického řešení a řešení získaného pomocí metody konečných prvků s velmi jemnou sítí konečných prvků. Na závěr je formalismus modifikován pro nepiezoelektrické materiály.

The diploma thesis deals with the design of the heating and cooling system, inclu-ding hot water preparation for the administrative part of the production hall. The first part of the thesis briefly discusses the history of heating and cooling of office buildings, legislative requirements and current solutions. The second part contains the design of the entire heating and cooling system with heat pump as a source of heat and cold. The final part of the thesis describes the experimental measure-ment and evaluation of the efficiency of the ice rink source.