Drought has an increasing importance in the Czech republic. It is necessary to look for potential irrigation water sources, which are important for agriculture. Treated wastewater can be one of them. It has potentially negative impact on soil physico-chemical properties, which is well-known from many researches in arid regions. The aim of this diploma thesis was to investigate, if the drip irrigation with treated wastewater (from constructed wetland) also has a potentially negative impact on two soils in the Czech republic. In one year experiment drip irrigation with treated wastewater was carried out in the greenhouse. Four treatments of irrigation was used for both soils - irrigation with treated wastewater, irrigation with groundwater, irrigation with treated wastewater + rainwater, irrigation with groundwater + rainwater. An amount of rainwater correspond with total precipitation of two locations, where the soils were brought from (Kostelec nad Ohří - Ústí nad Labem region and Hostětín - Zlín region). pH and electrical conductivity of soil leachate, total concentration of Na, K, Ca, Mg a P, bulk density, particle density and porosity was measured in both soils in 5 and 10 centimeters from source of irrigation. Values of pH of soil leachate fluctuated in every treatment in both soils during the...

There are many compounds that are used or produced by humans, e.g. pharmaceuticals and personal care products (PPCPs) which are used in large quantities around the world. These compounds end up in waste waters and enter sewage treatment plants (STPs). But not all of these compounds are removed during the treatment processes in STP and they enter the aquatic environment via sewage effluents. The thesis is focused especially on evaluation of presence and potential toxicological effects of PPCPs in aquatic environment. The risk assessment for a single compound is usually based on laboratory experiments. The first tested compound was verapamil, a pharmaceutical for lowering blood pressure and treatment of cardiac diseases. Two types of tests were carried out, an acute one with high concentration levels and a sub-chronic one with levels that included environmentally relevant concentration. After the exposure, common carp larvae and embryos showed malformation and edemas and the heart rate was decreased. According to 96hLC50, the verapamil could be classified as a harmful or toxic substance. The second tested substance was UV filter 2 phenylbenzimidazole-5-sulfonic acid which was found at relatively high concentrations in south Bohemian surface waters. In spite of very low bioconcentration of PBSA in fish, activities of 7-ethoxyresorufin-O-deethylase, 7-methoxyresorufin-O-deethylase and 7-penthoxyresorufin-O-deethylase in exposed rainbow trout were increased which indicate that activity of cytochromes P450 were affected even in environmentally relevant concentration. In a real aquatic system, fish are exposed to waters which contain not only a single pollutant but a mixture of them. A consequent experiment was carried out on rainbow trout caged in effluent for 13 days from STP in Gothenburg, the second biggest STP in Sweden. The concentration of selected antidepressants was measured in different fish tissues and plasma. Citalopram, sertraline and venlafaxine were found in fish brain and liver. As these antidepressants are used for the treatment of psychical disorders, behavior of fish could be affected. The limitations of these experiments, even with real water with a mixture of chemicals, are that fish are stressed in aquaria or cages and the exposure via the food chain is not included. Benthic organisms are an important part of fish diet. The impact of pollution on benthic organisms is often studied. However, there is limited knowledge about the bioaccumulation of pharmaceuticals in the benthos and consequently in fish. Therefore bioaccumulation in Hydropsyche and Erpobdella was studied. These organisms were collected in non-polluted and in effluent affected localities. Antibiotics azithromycin and clarithromycin, cardio-vascular drug verapamil, antifungal drug clotrimazole and antidepressants citalopram and sertraline were found in Hydropsyche. Cardio-vascular drug valsartan, antidepressant sertraline, anti-inflammatory drug diclofenac and antifungal drug clotrimazole were found in Erpobdella. Passive sampling is an integrative sampling method which allows the obtaining of time average concentration over period of few weeks. POCIS (Polar Organic Chemical Integrative Samplers) are used for sampling of polar compounds. It also can mimic bioconcentration. Based on the field experiments, the sampling rate for individual pharmaceuticals and illicit drugs was calculated. Twenty-one localities in the Czech Republic were sampled by POCIS and the water concentration of psychoactive drugs was estimated. Further research is necessary to make POCIS passive sampling a more precise tool for monitoring pharmaceutical and drug contents in aquatic environments.

Before the regulation of opium as a “narcotic” in Sweden in 1923, opium was not regulated for its intoxicating properties and was freely available. But not in any kind of shop. Opium was legally available only through the pharmacies. This thesis explores how this free availability of a narcotic was understood by its traders, the pharmacists. The title of this thesis – The Value of Poison – indicates how opium could be conceptualized both as a safe, everyday remedy essential to keep freely available and as a drug of intoxication. As a poison it could be articulated as a matter of primarily pharmacological, not moral or medical, concern. This also gave the pharmacists, with their special knowledge of pharmaka (drugs, poisons), an autonomous space of knowledge free from the ever more intruding “medical gaze”. But, in order to articulate this kind of understanding of opium, another kind of knowledge was needed to be acknowledged: that of the user. In this articulation a “sensus communis” was tied in with a broader cultural knowledge of drugs. Problems with opium were focused on the danger of acute poisoning, not recreational intoxication. Concepts that could have problematized this kind of use were rearticulated as problems either of illegitimate trade, unregulated markets and advertising or of draconian regulation by greedy or sloppy doctors. These rather opposite elements were made equivalent through the articulation of ignorance in both cases, thus further emphasizing the special knowledge of the pharmacist. The thesis locates a process of contradiction that contributes to the eventual diminishing of the discourse of poison towards the end of the period. The pharmaceutical knowledge that guaranteed the discourse was based on a “pharmaceutical gaze” on pharmaka. It pierced through the drug to identify its constituent parts. In this process it was promised that the different effects of opium would be separated. “Narcotic” could be a by-product, to be discarded or controlled, without dispensing of other therapeutic effects. With this ever deeper knowledge of opium, knowledge in the pharmacies was made insufficient for the full understanding or opium, and so too was that of the traditional user. The era of opium as a poison was over. / Före den första särlagstiftningen om narkotika i Sverige 1923 reglerades inte opiumets rusgivande egenskaper. Drogen var fritt tillgänglig i handeln. Men inte i vilken butik som helst. Opium kunde bara köpas lagligt på landets apotek. Den här avhandlingen undersöker hur denna fria tillgänglighet av narkotika förstods av droghandlarna själva, apotekarna. Titeln pekar på hur opium på en och samma gång kunde tänkas som en säker husmedicin vars tillgänglighet var avgörande för folkhälsan och som en rusgivande drog. Som ”gift” artikulerades det som en i första hand farmakologisk angelägenhet, inte en moralisk eller medicinsk. När de talade på detta sätt upprättade apotekarna, genom sin särskilda kunskap om farmaka, ett eget rum för sitt vetande, fritt från läkarnas allt mer genomträngande ”kliniska blick”. Men för att kunna artikulera denna förståelse av opium krävdes också att en annan typ av kunskap vidkändes: brukarens. Genom denna artikulation knöts brukarnas ”sensus communis” samman med en bredare kulturell kunskap om droger. De av opiumets problem som lyftes fram handlade om akut förgiftning, inte rekreationellt rusbruk. De begrepp som hade varit möjliga att användas för att problematisera denna senare form av bruk reartikulerades: antingen förpassades de till den olagliga handeln, de oreglerade marknaderna och reklamen, eller också till de drakoniska regleringarna som giriga och slarviga läkare stod bakom. Apotekarna artikulerade dessa båda helt motstående element som ekvivalenta genom en brist på kunskap, vilket i sin tur ytterligare stärkte deras egen kunskapsmakt. Avhandlingen lokaliserar även en processande motsägelse som sker när giftets diskurs tynar bort vid slutet av den undersökta perioden. Den farmaceutiska kunskap som underbyggde diskursen vilade på en ”farmaceutisk blick” på farmaka. Denna genomborrade drogämnet för att avslöja dess beståndsdelar. Genom denna process utlovades att opiumets olika effekter skulle kunna skiljas från varandra. ”Narkotikan” kunde ses som en bieffekt, som kunde kastas åt sidan eller kontrolleras separat, utan att opiumets kvarvarande terapeutiska effekter minskade. Denna allt djupare kunskap medförde att de enskilda apotekarnas eget vetande på apoteken inte räckte till för att fullt ut förstå opium, och därmed bröts även samartikulationen med brukarnas kunskap. Tidseran när opium var ett gift tog därmed slut under mellankrigsperioden.