Bakgrund: Kvinnlig könsstympning (female genital mutilation [FGM]) berör ca 100 till 140 miljoner kvinnor i världen. Varje år är det ca 3 miljoner flickor mellan åldrarna 0 till 15 år som riskerar att bli könsstympade.  Könsstympning förekommer i 26 länder belägna i Afrika, Mellanöstern & Asien. Att könsstympa kvinnor är en sed som är djupt inrotad i den spirituella och kulturella synen.  Enligt Svensk lag är all form av könsstympning olagligt och får ej utföras. Syfte: Syftet med denna litteraturstudie var att beskriva kvinnors upplevelse och erfarenheter av att leva med könsstympning samt deras möte med västerländsk sjukvård. Syftet var även att beskriva studiernas undersökningsgrupp i de inkluderade artiklarna. Metod: För att svara på föreliggande studies syfte har författarna gjort en beskrivande litteraturstudie. Litteraturstudien har 11 stycken artiklar som är av kvalitativ ansats, 1st som är av kvantitativ ansats och 3st som är av både kvalitativ och kvantitativ ansats, dessa analyserades objektivt. Databaserna Pubmed och CINAHL användes. Resultat: Kvinnorna i studien förklarar att det är tradition, kultur och en heder gentemot familjen att könsstympas. Känslor som rädsla, oro och skam är upplevelser kvinnorna förklarar vid mötet med västerländsk sjukvård, det kan vara i form av kommunikationsbrist och dåligt bemötande. Kvinnorna anser att det finns för lite kunskap om könsstympning inom västerländsk sjukvård och att detta behöver bli bättre. Positiva erfarenheter som kvinnorna tar upp är att de blev bemötta med respekt och god omvårdnad. Slutsats: Kvinnorna som genomgått könsstympning har både positiva och negativa erfarenheter av att vara könsstympad. Vid mötet med västerländsk sjukvård visar studier att rädsla och oro kan uppstå hos kvinnor som genomgått könsstympning. De positiva erfarenheter kvinnorna hade i mötet med sjukvården var att de kände sig bemötta med respekt av sjukvårdspersonalen som hade goda kunskaper om könsstympning. Genom ökad kunskap hos sjukvårdspersonal kommer kvinnornas oro och rädsla succesivt minska i de länder där könsstympning ej förekommer. / Background: FGM (female genital mutilation [FGM]) affects approximately 100 million to 140 million women worldwide. Each year there are about 3 million girls between the ages of 0-15 years who risk being genitally mutilated. Genital mutilation is present in 26 countries located in Africa, the Middle East & Asia. To circumcise women is a practice that is deeply rooted in the spiritual and cultural vision. According to Swedish law, all forms of genital mutilation are illegal and may not be performed. Aim: The purpose of the study was to describe women’s experience and the experience of living with genital mutilation and their encounter with western health care. The purpose was also to describe the studies finding mission in the included articles. Method: In order to answer to the present studies aim, the authors conducted a descriptive literature study. The literature have 11 articles that are of qualitative approach, 1 has the quantitative approach and 3 have both qualitative and quantitative approach, and they were analysed objectively. PubMed and CINAHL were used in this study. Results: The women in the study, explains that it's tradition, culture and an honour to the family being mutilated. Emotions such as fear, anxiety and shame are the experiences the women explained when meeting with western health care, and these feelings are related to the lack of communication and treatment. The women believe that there is too little knowledge about genital mutilation in the western health care and that needs to be better. Positive experience was also where the women are talking about that they were treated with respect and a good nursing care. Conclusion: The women who have undergone genital mutilation has both positive and negative experiences of being circumcised. Studies show that women who undergone female genital mutilation experience fear and anxiety while coming in contact with western health care. The positive experiences women had in the meeting with the western health care was that they felt treated with respect by medical staff who had good knowledge about female genital mutilation. Through increased knowledge among medical staff will the womens worry and fear gradually decrease in the countries where female genital mutilation not occur.

Entre 100 et 140 millions de femmes, de petites filles et d’adolescentes sont excisées (Andro et Lesclingrand, 2007). Les risques sanitaires de l’excision sont élevés et concernent la santé reproductive, physique et psychologique des femmes. Les nouvelles migrations et l’augmentation des pays qui légifèrent l’excision ont contribué à l’internationalisation de l’excision et à la modification de son processus. On constate actuellement une tendance de l’excision à devenir une pratique clandestine et une perte de sa signification rituelle. En même temps, les mouvements de lutte internationaux, régionaux autant que nationaux prennent de l’ampleur et connaissent une période de mutation afin de contrer la nouvelle figure de l’excision. Le Burkina Faso ne fait pas exception. Le gouvernement burkinabé s’est clairement positionné en faveur du mouvement de lutte contre l’excision et met en place de nombreux dispositifs juridiques, politiques et économiques afin d’en soutenir les initiatives. En 2003, 77 % des femmes burkinabè âgées de 15 à 49 ans se déclaraient excisées. Parallèlement, on assiste à une diminution de la pratique chez leurs filles entre 1998 et 2003 et à une augmentation du nombre de Burkinabè se déclarant contre la pratique. Pourtant en 2003, environ 40 % des femmes ont excisé ou souhaitent exciser leurs filles et environ 24 % des hommes et 26 % des femmes sont encore favorables à la perpétuation de l’excision. Ce mémoire s’intéresse d’abord aux changements de pratique, de connaissance et d’attitudes par rapport à l’excision entre 1998 et 2003. Il s’intéresse ensuite aux déterminants socioculturels, démographiques et économiques favorisant la persistance de cette pratique au sein de la société burkinabé et aux obstacles rencontrés par les intervenants pour combattre l’excision sur le terrain. Pour ce faire la recherche associe méthodes quantitatives et qualitatives. Elle combine analyses statistiques des données des enquêtes démographiques de santé de 2003 et de 1998 et analyse des données d’entretiens collectées auprès d’acteurs sur le terrain entre le premier et le 10 octobre 2005. / Between 100 and 140 million women, young and teenage girls have undergone feminine genital cutting (Andro and Lesclingrand, 2007). The medical risks of excision are high and relate to the reproductive, physical and psychological health of women. New migrations and the increase of countries legislating female genital cutting contributed to the internationalization of the practice and to the changes in its process. The loss of the ritual significance of excision and the increase as a concealed practice have clearly been noticed. In the meantime, the international movements fighting it, regional as well as national, amplify and put changes together in order to counter the new figure of female genital cutting. Burkina Faso does not make exception. The Government has clearly positioned itself in favour of movements fighting excision; and has set up many legal, political and economic devices in order to support their initiatives. In 2003, 77% of Burkina Faso women aged between 15 to 49 years old were declared excised. In parallel, a reduction in the practice for their daughter between 1998 and 2003 is noted as well as an increase in the number of Burkina Faso people declaring themselves against the practice. However in 2003, approximately 40% of women have performed or would like their daughter to undergo the cut; and approximately 24% of men and 26% of women are still encouraging the perpetuation of excision. This thesis’ first concern is the changes in the practice, and the knowledge and attitudes regarding excision between 1998 and 2003. Secondly, the attention is drawn on the socio-cultural, demographic and economic determinants indulging the doggedness of this practice within Burkina Faso people and on the concrete obstacles workers encounter on the ground. Therefore this research associates quantitative and qualitative methods. It combines statistic analysis of the Demographic and Health Surveys of 2003 and 1998 data; and analyzes data collected from talks made between October 1 st and 10th, 2005 with fields’ workers.

This thesis explores the social and political relations of an international non-governmental organisation (NGO) based in Senegal. NGOs and international development have been the subject of research from a number of different perspectives, including the politics (and anti-politics) of development, post-development, structural violence and the ‘everyday lives' of NGO participants and workers (Ferguson 1990; Escobar 1995; Farmer 2004; Bornstein 2005; Hilhorst 2003). The present study builds on this scholarship through an ethnographic exploration of the networks of people involved with Tostan, an American NGO based in Senegal whose developmental objective is to engender social change among rural groups in Senegal (particularly those that practice female genital cutting), using a human rights education framework. Through identification and scrutiny of the organisation's macro- and micro-level social relations, I critically examine how ‘development' operates as a cultural and political process. I focus analytically on conceptions of knowledge and ignorance, particularly the ways in which these constructions are acted upon and utilised by different actors within the organisation. I argue that, as an NGO (and thus a ‘moral actor,' Guilhot 2005: 6) within the contemporary donor-driven development industry, a key preoccupation for Tostan as an organisation is the management of perception, or a concern for the ‘spectacle of development' (Allen 2013). Flowing from this argument is the assertion that the activities carried out by actors at every level of the organisation to produce and re-produce particular narratives through strategic knowing and unknowing are as significant (if not more so) as the formal programmatic activities implemented by the organisation ‘on the ground.' As David Mosse argues, development involves not only social work, but also the conceptual work of ‘enrolment, persuasion, agreement and argument that lies behind the consensus and coherence necessary to sustain authoritative narratives and networks for the continued support of policy' (Mosse 2005: 34). As I argue here, NGO actors work to (re)produce, project and protect particular narratives, through the strategic exercise of knowledge and ignorance, in order to access or consolidate positions of power within the politics of aid. Drawing on critical theories of development and human rights (e.g. Sachs 1992; Escobar 1991, 1995; Guilhot 2005, inter alia), within a political context succinctly described by Ellen Foley (2010: 9) as ‘the neoliberalization of just about everything,' I explore how actors across the organisation are linked in a web of cultural and political presuppositions, values, and motivations.

This thesis describes the phenomenon of female genital mutilation (FGM) in Northeastern Africa, focusing on Egypt, Somalia and the Sudan. The core of the thesis lies in literary reflections of this practice and analysis of literary works tackling this subject. Presenting a wide range of literary works on the subject, this thesis aims at classifying and comparing them, with a special emphasis on the works of Nawal el Saadawi and Nuruddin Farah. The thesis also presents a summary of current local and international laws on FGM as well as a list of organisations dealing with FGM. It also briefly describes the religious background of FGM and current situation in Egypt and the Sudan.

Résumé de la thèseLa thèse porte sur l‘analyse des apports de la communication dans la lutte contre des mutilations corporelles, en l‘occurrence celles de l‘excision (qui est la pratique la plus répandue) des femmes en République de Guinée. Ces pratiques de mutilations corporelles étant encore souvent présentées comme des pratiques culturelles inscrites dans la tradition et l‘identité culturelles. L‘approche «Garder le rite, pas la mutilation » fait la promotion des rites de l‘excision, de la clitoridectomie et de l‘infibulation, c'est-à-dire celle des valeurs qui sont léguées aux jeunes filles pendant la cérémonie initiatique. L‘Assemblée générale des Nations Unies a donc consacré en 2012 et 2014 deux (2) importantes résolutions à la lutte contre les mutilations génitales féminines (le 20 décembre 2012, la Résolution A/RES/67/146 et en décembre 2014, la Résolution A/RES/69/150). Pour notre part, à travers ce travail de recherche, nous n‘avons fait ni la promotion du rite, puisque de meilleures voies de transmission de la culture existent aujourd'hui, ni celle de la mutilation à cause de ses effets sur la santé de la reproduction. Nous avons essayé de comprendre, à partir des grilles d‘intelligibilité communicationnelles, comment ces pratiques peuvent évoluer et avons préconisé certaines solutions en mobilisant des approches dans le champ des Sciences de l‘information et de la communication. Il a donc été nécessaire de réformer la « communication » pour la mettre au centre des préoccupations des gouvernants en vue d‘amener les pratiquants à un changement culturel consistant à abandonner progressivement des pratiques vieilles de plusieurs milliers d‘années. / Summary of the thesis The thesis focuses on the analysis of the contribution of communication in the fight against bodily mutilations, in this case that of excision or female circumcision (which is the most widespread practice) of women in the Republic of Guinea. These practices of bodily mutilations are still often presented as cultural practices inscribed in cultural tradition and identity. The "keep the rite, not the mutilation" approach promotes the rites of excision, clitoridectomy and infibulation, the values that are bequeathed to young girls during the initiation ceremony. In 2012 and 2014, the United Nations General Assembly devoted two (2) important resolutions to the fight against female genitale mutilations (on December 20, 2012, Resolution A / RES / 67/146 and in December 2014, Resolution A / RES / 69/150). For our part, through this research work, we have neither promoted the rite, since better ways of transmitting culture exist today, nor that of mutilation because of its effects on the health of the reproduction. We have tried to understand, from the grids of communication intelligibility, how these practices can evolve and have advocated some solutions by mobilizing approaches in the field of the sciences of information and communication. It was therefore necessary to reform the "communication" to put it at the center of the concerns of the rulers with a view to bringing practitioners to a cultural change of gradually abandoning practices that are several thousand years old.

<p>RÉSUMÉ</p><p>Au Sénégal, on estime que 28% des femmes sont victimes de MSF et les Diolas sont une des ethnies au Sénégal qui pratique cette tradition. Dans ce mémoire, j’ai l’intention en premier de comprendre et d’interpréter les raisons qui justifient, selon les Diolas, cette pratique par la méthode d’abduction et selon la théorie du néo-institutionnalisme. Mon intention en second lieu est de comprendre les éléments de la prétendue diminution de cette pratique dans certaines zones chez les Diolas. Je veux aussi comprendre le rôle de l’ONG Tostan dans la réduction de ce phénomène. Pour réaliser mon projet d’étude, j’ai fait mon étude de terrain dans deux villages au sud du Sénégal.</p><p>Le résultat que j’ai obtenu est que les Diolas prennent les MSF comme étant la première phase, c'est-à-dire la première partie du cycle d’initiation d’une femme. Une fille ne peut pas entrer dans la deuxième partie de son cycle d’initiation qui est une partie essentielle pour réussir dans la société Diolas si elle ne passe pas la première partie grâce aux MSF. En plus, j’ai compris que les raisons principales pour justifier cette pratique sont que les Diolas pensent que c’est une recommandation islamique. Donc, la pratique des MSF est venue avec l’islamisation.</p><p>Après les programmes habituels de Tostan dans les villages, les deux villages de mon étude de cas ont fait leur déclaration publique contre les MSF. Selon les villageoises, dans le premier village, le rôle de Tostan est qu’elle les avait aidées à sensibiliser et à stabiliser le village dans l’abandon de la pratique. Mais il faut souligner que le procès d’abandon avait déjà commencé avant l’arrivée de Tostan. Je trouve qu’il y avait des éléments nécessaires pour cesser la pratique qui a existé dans le village avant l’arrivée de Tostan qui a facilité le choix de vraiment laisser la pratique.</p><p>Le deuxième village témoigne de l’importance de Tostan et affirme que sans Tostan, le village n’aurait pas abandonné la pratique des MSF. En même temps, il y a des indicateurs qui montrent que tous les habitants du village n’ont pas cessé la pratique.</p><p>Une remarque inattendue est que la norme féminine est sous transformation. Aujourd’hui, les femmes non excisées sont mieux considérées que les femmes excisées par toute la société dans les villages de cas de cette étude. Une autre observation intéressante est que les villageoises témoignent que les jeunes femmes étaient les plus difficiles à convaincre d’abandonner la MSF contrairement aux femmes plus âgées alors que se sont elles, qui peuvent fondamentalement réclamer d’avoir été préjudiciées a cause de nous au Nord, il est normalement difficile de moduler les différentes traditions à cause des anciennes générations au sein de la société.</p> / <p>An estimated 28% of Senegalese women are victims to Female Genital Mutilation (FGM). The Jola tribe in Senegal is one of the ethnics that practice this tradition. In this thesis, I have the intention to interpret and try to understand the reasons the Jolas have to justify this practice. This will be made by an abdication method with the help of the New Institutional Theory. My purpose is furthermore to understand why the practice of FGM has reduced in some Jola-zones. Furthermore, I would like to comprehend the role of the NGO Tostan in the reduction process. To fulfil my intentions, I have made a Minor Field Study of two case studies conducted in two villages in the south of Senegal.</p><p>The result of this thesis is that the Jolas use FGM as the first phase of the women’s initiation cycle. A girl is not allowed to enter the second phase of her initiation cycle, which is an essential step in order to succeed in the Jolas society, if she has not already passed the first phase. I have understood that the main reason to justify the practice is that the Jolas think that FGM is an Islamic decree. This signifies that the practice of FGM has come to the Jolas with the Islamization.</p><p>After conducting ordinary programs of Tostan in the villages, both case villages have made an official declaration against FGM. According to the villagers in the first village, the role of Tostan was to help them be aware and stabilise so that they could implement the declaration. It is important to stress that the process of abandoning the practice had already started before the intervention of Tostan in this village. This study shows that there already existed certain important elements in the village that had facilitated the work of Tostan.</p><p>The second village confirmed the importance of Tostan and affirmed that they would not have abandoned the practice without the intervention of Tostan. On the other hand, I found indications which show that all the habitants of the village have not yet abandoned the practice.</p><p>One unexpected observation that I achieved to track during my fieldwork is that the female norm is under transformation. Today, the non-circumcised women are considered more attractive than circumcised women. Another interesting observation is that the villagers claimed that it is actually more complicated to convince the younger female generations to abandon the practice of FGM, and not the elder females, which basically can be claimed to be the prejudice we carry with us from the North, when talking about different traditions which are difficult to change because of the old generations in a society.</p>

Entre 100 et 140 millions de femmes, de petites filles et d’adolescentes sont excisées (Andro et Lesclingrand, 2007). Les risques sanitaires de l’excision sont élevés et concernent la santé reproductive, physique et psychologique des femmes. Les nouvelles migrations et l’augmentation des pays qui légifèrent l’excision ont contribué à l’internationalisation de l’excision et à la modification de son processus. On constate actuellement une tendance de l’excision à devenir une pratique clandestine et une perte de sa signification rituelle. En même temps, les mouvements de lutte internationaux, régionaux autant que nationaux prennent de l’ampleur et connaissent une période de mutation afin de contrer la nouvelle figure de l’excision. Le Burkina Faso ne fait pas exception. Le gouvernement burkinabé s’est clairement positionné en faveur du mouvement de lutte contre l’excision et met en place de nombreux dispositifs juridiques, politiques et économiques afin d’en soutenir les initiatives. En 2003, 77 % des femmes burkinabè âgées de 15 à 49 ans se déclaraient excisées. Parallèlement, on assiste à une diminution de la pratique chez leurs filles entre 1998 et 2003 et à une augmentation du nombre de Burkinabè se déclarant contre la pratique. Pourtant en 2003, environ 40 % des femmes ont excisé ou souhaitent exciser leurs filles et environ 24 % des hommes et 26 % des femmes sont encore favorables à la perpétuation de l’excision. Ce mémoire s’intéresse d’abord aux changements de pratique, de connaissance et d’attitudes par rapport à l’excision entre 1998 et 2003. Il s’intéresse ensuite aux déterminants socioculturels, démographiques et économiques favorisant la persistance de cette pratique au sein de la société burkinabé et aux obstacles rencontrés par les intervenants pour combattre l’excision sur le terrain. Pour ce faire la recherche associe méthodes quantitatives et qualitatives. Elle combine analyses statistiques des données des enquêtes démographiques de santé de 2003 et de 1998 et analyse des données d’entretiens collectées auprès d’acteurs sur le terrain entre le premier et le 10 octobre 2005. / Between 100 and 140 million women, young and teenage girls have undergone feminine genital cutting (Andro and Lesclingrand, 2007). The medical risks of excision are high and relate to the reproductive, physical and psychological health of women. New migrations and the increase of countries legislating female genital cutting contributed to the internationalization of the practice and to the changes in its process. The loss of the ritual significance of excision and the increase as a concealed practice have clearly been noticed. In the meantime, the international movements fighting it, regional as well as national, amplify and put changes together in order to counter the new figure of female genital cutting. Burkina Faso does not make exception. The Government has clearly positioned itself in favour of movements fighting excision; and has set up many legal, political and economic devices in order to support their initiatives. In 2003, 77% of Burkina Faso women aged between 15 to 49 years old were declared excised. In parallel, a reduction in the practice for their daughter between 1998 and 2003 is noted as well as an increase in the number of Burkina Faso people declaring themselves against the practice. However in 2003, approximately 40% of women have performed or would like their daughter to undergo the cut; and approximately 24% of men and 26% of women are still encouraging the perpetuation of excision. This thesis’ first concern is the changes in the practice, and the knowledge and attitudes regarding excision between 1998 and 2003. Secondly, the attention is drawn on the socio-cultural, demographic and economic determinants indulging the doggedness of this practice within Burkina Faso people and on the concrete obstacles workers encounter on the ground. Therefore this research associates quantitative and qualitative methods. It combines statistic analysis of the Demographic and Health Surveys of 2003 and 1998 data; and analyzes data collected from talks made between October 1 st and 10th, 2005 with fields’ workers.

xiv, 246 p. : ill. A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. / This project is a critical and comparative investigation of Western and non-Western practices of body modification. Situated in the realm of feminist political theory, the project engages the literature and debates concerning embodiment, or the symbolic and concrete meanings of women's bodies. I specifically explore two examples of the physical construction of women's bodies: breast implantation in the United States and female genital cutting (FGC) in Senegal. I demonstrate that each of the practices molds bodies into preexisting naturalized forms. For this project, I conducted eighty in-depth, open-ended, and semi-structured interviews with women and men in twelve different locations in Senegal. Then, I carried out sixty-five in-depth, open-ended, and semi-structured interviews with American men and women from twenty-one different cities. I argue that the information that emerges from looking at body normalization comparatively allows me to make two important claims. The first is that the material that originates from interviews in this comparative study disrupts existing hegemonic discourse on sex-based body modifications. In particular, the comparative findings challenge the viewpoint that espouses a "Western women are free, African women are oppressed" binary. Second, examining FGC in Senegal alongside breast implantation in the US can uncover normalization that is invisible within social fields, or in the lives of women and men. Normalization is hard to see when in it, but easier to see if an individual steps outside of herself, her context, and her patriarchy. Thus, though many women do not recognize the normalizing structures within their own lives, they often are able to see these hegemonic structures in the lives of others. Women stepping outside of their own contexts can provide fresh, critical eyes that recognize embedded normalizations and oppression in other contexts. Further, this realization also can push them to return that critical gaze onto their own environment, which is the beginning of locating mechanisms of control within their own field. The construction of sex and the imprinting of gender norms upon bodies are manifestations of regulation and normalization that occur within socio-cultural contexts, and which individuals can potentially locate through a comparative conversation of this type. / Committee in charge: Dennis Galvan, Chairperson, Political Science; Julie Novkov, Member, Political Science; Leonard Feldman, Member, Political Science; Stephen Wooten, Outside Member, Anthropology

The purpose of my work is to understand and examine the reasons why Ethiopia has not developed a larger decline of female genital mutilation, despite their ban on it? A ban that has been operating for ten years should reasonably have reached a greater change than the one Ethiopia has developed today. Based on two different branches of feminist theory, the liberal feminist theory and radical feminist theory, I will try to understand the potential power relationship that can be a immense reason for Ethiopia's continued practice with regard to female genital mutilation.   I will examine the liberal feminist approach when it comes to seeing the state as the source of the balance of power that generate inequality in the world between men and women. I will also apply the radical feminist theory on my case study and understand the problem of patriarchy and its already set roles for men and women that we are following in the society today, resulting in gender inequality.   The result shows that the radical feminist approach with patriarchy as essential explanation, which articulates that because of ancient traditions and the exercise of power, the amendment must be the changing of power relations between men and women in the private sphere rather than the liberal feminist approach which applies that the state repair the problem.

The phosphorylation and dephosphorylation of the tyrosine amino-acids in proteins play an important role in the regulation of many cellular processes in all eukariotic organisms, including the regulation of cell cycle control, growth control, cellular differentiation and gene and synaptic transmission. The involvement of the phosphatase genes in human carcinogenesis was long-suspected, but PTEN is the first important phosphatase gene proven to be a true tumour suppressor. The basic function of normal PTEN is the dephosphorylation of the kinases and inhibition of the integrin and growth factor mediated kinase signalling pathways. The central hypothesis of this study is that PTEN plays an important role in tumours of the upper female genital tract. The involvement of aberrations in the coding regions of this gene was studied in specific gynaecologic tumours and tissues using polymerase chain reaction based mutation analysis. The research model was to study both the malignant tumour and the closest available pre-malignant or benign counterpart to demonstrate different levels of involvement of PTEN in the evolving steps. The PTEN gene was found to be intimately involved in endometrial carcinogenesis. Involvement was demonstrated in hyperplasia and was common in endometroid carcinoma (54%). Pathogenic PTEN mutations were much more common in cancer than in hyperplasia (10%). Multiple mutations were found in some late stage tumours, suggesting that the already malignant tumour cells accumulate more genetic mutations over time. All tumours with more than one pathogenic mutation occurred in African patients. The latter twofindings are unique to the current study. Selective involvement of the PTEN gene was demonstrated in uterine soft tissue tumours. PTEN involvement was neither found in benign soft tissue tumours nor significantly in leiomyosarcoma or endometrial stromal sarcoma. However, PTEN plays a significant role in uterine carcinosarcoma (13%) and specifically in tumours with an endometroid epithelial component, where mutations were found in 17%. This finding is a highly significant and unique research result which supports the hypothesis of the endometrial origin of these tumours. It also supports the observation of a strong link between this gene and endometroid differentiation, with morphology strongly linked to cellular genetics. PTEN gene mutation was demonstrated in ovarian endometroid carcinoma in ~29% of cases investigated. This finding confirms PTEN involvement in carcinogenesis in this tumour type. The finding suggests that PTEN involvement is linked to endometroid epithelial morphology. We could not sufficiently test the involvement of the gene in benign or pre-malignant ovarian endometroid lesions and thus cannot comment on the chronology of mutations in this tissue type. When all tumour types were included, there was a tendency towards a lower frequency ofPTEN mutations in African women. PTEN mutations correlated with endometroid histology. In combination, these results confirm the racial disparity in tumour type distribution or morphology. In summary this study demonstrated significant though highly selective PTEN gene involvement and a strong and interesting association between genotype and histological phenotype was confirmed. The findings enhance our understanding of carcinogenesis and should lead to translational research into new anti-neoplastic drugs. AFRIKAANS: Fosforilering en defosforilering van die tirosien aminosure in proteine speel ‘n belangrike rol in die regulering van sellulêre prosesse in alle eukariotiese organismes. Dit sluit die regulering van selsikluskontrole, groeikontrole, sellulêre differensiasie sowel as genetiese en sinaptiese oordrag in. Dit word lank reeds gespekuleer dat die fosfatase-gene betrokke is in menslike karsinogenese, maar die PTEN geen is die eerste fosfatase geen wat bewys word om ‘n ware tumoronderdrukker geen te wees. As basiese funksie defosforileer normale PTEN die kinases en inhibeer dit die kinase sinjaal kontrolepaaie wat deur integrien en groeifaktor beheer word. Die sentrale hipotese van hierdie studie is dat PTEN ‘n belangrike rol speel in tumore van die boonste genitale traktus. Die frekwensie van abnormaliteite in die koderingsareas van hierdie geen is bestudeer in spesifieke ginekologiese tumore en weefsels met die gebruik van polimerase kettingreaksie gebaseerde mutasie-analise. Die maligne tumore sowel as die mees verwante pre-maligne of benigne weefsel- of tumortipes wat beskikbaar was, is gebruik as navorsingsmateriaal om sodoende die verskillende vlakke van PTEN betrokkenheid in die ontwikkeling van neoplasie te demonstreer.Intieme betrokkenheid van die PTEN geen is gevind in endometriële karsinogenese. PTEN mutasies is in hiperplasie gevind en dit was algemeen in endometroiede karsinoom (54%). Patogene mutasies was baie meer algemeen in kanker as in hiperplasie (10%). Veelvuldige mutasies is in sommige laat stadium tumore aangetoon, wat suggereer dat reeds maligne selle meer genetiese mutasies oor tyd verkry. Alle tumore waar meer as een patogeniese mutasie gevind is het voorgekom by swart pasiënte. Die laaste twee bevindinge is uniek tot hierdie studie.Selektiewe betrokkenheid van die PTEN geen is gevind in die ontwikkeling van sagte weefsel tumore van die uterus. PTEN mutasies is nie in benigne sagte weefsel tumore gevind nie en geen betekenisvolle betrokkenheid is in leiomiosarkome of endometriële stromale sarkome aangetoon nie. PTEN was egter betekenisvol betrokke in karsinosarkome van die uterus (13%) en veral in tumore met ‘n endometrioiede epiteelkomponent waar mutasies in 17% gevind is. Hierdie bevinding is ‘n hoogs betekenisvolle en unieke navorsingsbevinding wat die hipotese ondersteun dat hierdie tumore uit die endometrium ontstaan. Dit onderskryf ook die indruk dat ‘n sterk band bestaan tussen hierdie geen en endometroiede differensiasie, met morfologie sterk gekoppel aan sellulêre genetika.Mutasie in die PTEN geen is aangetoon in ovariële endometroiede karsinoom in ~29% van gevalle wat ondersoek is. Die bevinding bevestig PTEN betrokkenheid in karsinogenese in hierdie tumortipe. Weereens toon die resultaat dat PTEN betrokkenheid gekoppel is aan endometroiede morfologie. Die ondersoek van benign of pre-maligne letsels in hierdie orgaan was nie voldoende om kommentaar oor die tydsberekening van mutasie te kan lewer nie.Met alle tumortipes in ag genome, is daar ‘n tendens aangetoon van minder PTEN mutasies in swart vroue. PTEN mutasies korreleer met endometroiede histologie. In kombinasie bevestig hierdie resultaat ‘n rasse-diskrepansie in die distribusie van tumourtipe of morfologie. In opsomming is die bevinding van hierdie studie dat daar betekenisvolle dog hoogs selektiewe PTEN geen betrokkenheid in boonste genitale traktus tumore is. ‘n Sterk en interessante verband is bevestig tussen genotipe en histologiese fenotipe. Hierdie resultate verbeter die begrip van karsinogenese en behoort ‘n bydrae te lewer in die soeke na nuwe anti-neoplastiese middels. / Thesis (PhD)--University of Pretoria, 2011. / Obstetrics and Gynaecology / unrestricted