Uma fase polimérica inédita de polidimetilsiloxano/carvão ativado (PDMS-ACB) é proposta como fase extratora para extração por sorção em barras de agitação (SBSE). A barra de PDMS-ACB foi desenvolvida no laboratório usando um molde de teflon simples, demonstrando estabilidade e resistência aos solventes orgânicos. Utilizando uma única barra de PDMS-ACB mais de 150 extrações foram realizadas sem qualquer dano. A barra de SBSE contém aproximadamente 100 µL de revestimento polimérico, 2,36 mm de diâmetro e 2,2 cm de comprimento. A barra PDMS-ACB foi aplicada na extração de seis pesticidas (ametrina, atrazina, bifentrina, carbofurano, metribuzim e tebutiurom), com propriedades predominantemente polares, comumente aplicados em plantações de cana-de-açúcar. A barra PDMS-ACB foi utilizada na determinação de pesticidas em garapa através de cromatografia gasosa hifenada à espectrometria de massa (GC-MS). O planejamento experimental foi empregado na etapa de otimização da extração SBSE, um planejamento fatorial fracionado avaliou a influência dos principais parâmetros envolvidos. Posteriormente, o planejamento composto central (CCD) com conformação estrela foi explorado para otimizar os fatores significativos na extração. Apenas 200 µL de acetato de etila foram utilizados como solvente na dessorção líquida (LD). Para os compostos avaliados, o método apresentou limite de quantificação (LOQ) no intervalo de 0,5 - 40 µg L-1, as recuperações variaram entre 0,18 - 49,50 % e a precisão intra-dia variou de 0,072 - 8,40 %. Concluída a etapa de validação, o método foi aplicado em amostras reais de garapa disponíveis comercialmente em São Carlos-SP. / A novel polydimethylsiloxane/activated carbon (PDMS-ACB) is proposed as new polymeric phases for stir bar sorptive extraction (SBSE). The PDMS-ACB was developed in lab using simple teflon\'s mold, demonstrating remarkable stability and resistance to organic solvents, over 150 extractions without any damage. SBSE bar contained 100 µL of polymeric coating, a diameter of 2.36 mm and a length of 2.2 cm. It was applied to the determination of pesticides (ametryn, atrazine, bifenthrin, carbofuran, metribuzine and tebuthiuron) having predominantly polar properties, applied in sugarcane crops. PDMS-ACB was employed in the determination of pesticides in sugarcane juice using gas chromatography coupled to mass spectrometry (GC-MS). Experimental design was employed in the optimization step, a fractional factorial evaluated the main parameters involved in the extraction procedure. Afterwards, central composite design (CCD) was exploited to optimize the significant factors on the extraction. About 200 µL of ethyl acetate were employed as solvent in the liquid desorption (LD). The method presented limit of quantification (LOQ) from 0.5 to 40 µg L-1, recoveries varied 0.18 - 49.50% and precision intra-day 0.072 - 8.40%. Hence, the method was applied to the analysis of real sugarcane juice samples commercially available in São Carlos-SP.

This dissertation presents a low-power high-resolution delta-sigma ADC. Two new architectural design techniques are proposed to reduce the power dissipation of the ADC. Compared to the conventional active adder, the direct charge transfer (DCT) adder greatly saves power by keeping the feedback factor of the active adder unity. However, the inherent delay originated from the DCT adder will cause instability to the modulator and complex additional branches are usually needed to stabilize the loop. A simple and power-efficient technique is proposed to absorb the delay from the DCT adder and the instability issue is therefore solved. Another proposed low-power design technique is to feed differentiated inverted quantization noise to the input of the last integrator. The modulator noise-shaping order with this proposed technique is effectively increased from two to three without adding additional active elements. The delta-sigma ADC with the proposed architectural design techniques has been implemented in transistor-level and fabricated in 0.18 µm CMOS technology. Measurement results showed a SNDR of 99.3 dB, a DR of 101.3 dB and a SFDR of 112 dB over 20 kHz signal bandwidth, resulting in a very low figure-of-merit (FoM) in its application category. Finally, two new circuit ideas, low-power parasitic-insensitive switched-capacitor integrator for delta-sigma ADCs and switched-resistor tuning technique for highly linear Gm-C filter design are presented. / Graduation date: 2012 / Access restricted to the OSU Community at author's request from June 9, 2012 - June 9, 2014

Three main analog circuit building blocks that are important for a mixed-signal system are investigated in this work. New building blocks with emphasis on power efficiency and compatibility with deep-submicron technology are proposed and experimental results from prototype integrated circuits are presented. Firstly, a 1.1GHz, 5th order, active-LC, Butterworth wideband equalizer that controls inter-symbol interference and provides anti-alias filtering for the subsequent analog to digital converter is presented. The equalizer design is based on a new series LC resonator biquad whose power efficiency is analytically shown to be better than a conventional Gm-C biquad. A prototype equalizer is fabricated in a standard 0.18μm CMOS technology. It is experimentally verified to achieve an equalization gain programmable over a 0-23dB range, 47dB SNR and -48dB IM3 while consuming 72mW of power. This corresponds to more than 7 times improvement in power efficiency over conventional Gm-C equalizers. Secondly, a load capacitance aware compensation for 3-stage amplifiers is presented. A class-AB 16W headphone driver designed using this scheme in 130nm technology is experimentally shown to handle 1pF to 22nF capacitive load while consuming as low as 1.2mW of quiescent power. It can deliver a maximum RMS power of 20mW to the load with -84.8dB THD and 92dB peak SNR, and it occupies a small area of 0.1mm2. The power consumption is reduced by about 10 times compared to drivers that can support such a wide range of capacitive loads. Thirdly, a novel approach to design of ADC in deep-submicron technology is described. The presented technique enables the usage of time-to-digital converter (TDC) in a delta-sigma modulator in a manner that takes advantage of its high timing precision while noise-shaping the error due to its limited time resolution. A prototype ADC designed based on this deep-submicron technology friendly architecture was fabricated in a 65nm digital CMOS technology. The ADC is experimentally shown to achieve 68dB dynamic range in 20MHz signal bandwidth while consuming 10.5mW of power. It is projected to reduce power and improve speed with technology scaling.

Entre le XIème et le XVIème siècle, la Mer des Indes fut le théâtre de nombreux mouvements populationnels aux fins essentiellement commerciales ou coloniales. Madagascar se trouve à la croisée des mondes asiatiques et africains. La côte sud-est malgache a vu l'arrivée de plusieurs migrations : la dernière, probablement vers la fin du XVème siècle, serait celle des Antemoro dont une partie d'entre eux se réclame d'une origine arabe et se rattache à La Mecque. L'éthnie des Antemoro a fait l'objet de nombreuses études anthropologiques et linguistiques. Néanmoins, le débat sur l'origine des migrants fait toujours l'objet d'hypothèses contradictoires. Leurs origines génétiques pourraient ainsi être l'Arabie, l'Afrique de l'Est, l'Inde ou encore l'Asie du Sud-Est à une époque où ces régions étaient déjà islamisées. Ce travail a consisté à étudier la diversité génétique d'une population Antemoro afin d'apporter des éléments de réponse à la question de leur origine biologique. Ce projet interdisciplinaire a pour objectif de mettre en relation l'anthropologie culturelle et sociale avec l'anthropologie biologique. Le polymorphisme du chromosome Y a été étudié afin de rechercher les origines des lignées paternelles par l'analyse de 17 marqueurs microsatellites ainsi que des mutations ponctuelles de l'ADN de la partie non recombinante du chromosome Y. De même, la variabilité génétique des lignées maternelles a été analysée par séquençage des régions hypervariables I et II de l'ADN mitochondrial, et par la définition de polymorphismes bialléliques dans sa région codante. Nous avons mis en évidence la présence de deux haplogroupes du chromosome Y chez certains groupes Antemoro, qui les différencient de la diversité habituellement rencontrée dans les populations malgaches. Bien que la majeure partie des Antemoro entre dans la diversité observée en Afrique sub-Saharienne et en Asie du Sud-Est, quelques haplotypes, des lignées paternelles, les lieraient au Moyen-Orient. Les lignées maternelles, quant à elles, ne les différencient pas de celles des autres populations malgaches. L'isolat génétique formé par certaines " pseudo-castes " Antemoro confirme bien l'isolat culturel. Ce travail apporte une nouvelle vision de la diversité génétique humaine à Madagascar.

Submitted by Chiara Fumagalli (fumachiara@gmail.com) on 2016-12-14T14:12:16Z No. of bitstreams: 1 Chiara Fumagalli MPGI Thesis.pdf: 1992906 bytes, checksum: 26dfbfc09c12655c54a59f69ab3369b5 (MD5) / Approved for entry into archive by Josineide da Silva Santos Locatelli (josineide.locatelli@fgv.br) on 2016-12-14T14:41:45Z (GMT) No. of bitstreams: 1 Chiara Fumagalli MPGI Thesis.pdf: 1992906 bytes, checksum: 26dfbfc09c12655c54a59f69ab3369b5 (MD5) / Made available in DSpace on 2016-12-14T15:03:26Z (GMT). No. of bitstreams: 1 Chiara Fumagalli MPGI Thesis.pdf: 1992906 bytes, checksum: 26dfbfc09c12655c54a59f69ab3369b5 (MD5) Previous issue date: 2016-11-16 / The Italian business presence in Brazil has been characterizing, since years, the economic landscape of Brazil. More than 1300 Italian companies, from the biggest to the smallest one, are acting in Brazil, exploiting the overall potential of this country and contributing with the excellence they can bring. Noting the consistency of the Italian presence and wondering how those companies have entered such a complex country and, a recurring name has been GM Venture, which became the protagonist of this dissertation. The thesis in fact aims at studying how GM Venture Company has been able to create value for Italian SMEs, contributing to their success in Brazil during the last ten years. Therefore this analysis focuses firstly on the literature dealing with the key success factors for the internationalization of small-medium enterprises. The second part of the literature goes deep in the specific case of the Brazilian context, trying to understand the major problems that this particular environment poses to small-medium companies internationalizing here and some specific factors Italian companies should pay attention to. Given this introduction, the analysis centers on the core topic of this dissertation. In fact the investigation regarding the GM Venture case looks specifically at how this company has been able create value despite those problems and create a business model able to successfully support SMEs entering Brazil. The inquiry on the case and the conclusion reports how GM Venture, through its operations, has been able to respond to the Brazilian rtise, and positively contribute to the growth and value of the Italian economic reality in this country. Moreover looking at the literature findings, this specific case introduces some novelty in the emerged factors, such as a new way of conceiving the Network, as well as new factors or undervalued ones for success such as promptness in the response and trustworthiness, which are essential in the Brazilian economic environment. / Desde muitos anos a presença Italiana no Brasil foi caracterizante do panorama econômico brasileiro. Mais de mil trezentos empresas italianas, das maiores ate as menores, são presente hoje no Brasil, aproveitando inteiramente do potencial Brasileiro e levando no Brasil uma contribuição relevante no sistema econômico, por meio da excelência que caracteriza estas empresas Italianas. Olhando ao tamanhão da realidade das empresas italiana no Brasil, e como todas essas corporações podia ter entrado em um pais muito complexo, ponto central foi a GM Venture, que se tornou o protagonista da minha dissertação. Esso TCC tem o escopo de entender melhor como esses deis anos GM Venture conseguiu em criar valor econômico por muitas media e pequena empresas Italianas, levando eles ao sucesso no novo país. Por isso a dissertação se focaliza inicialmente sobre a literatura da internacionalização e dos fatores essenciais de sucesso por uma pequena e media empresa em general. O secundo fator de analise nela literatura para o caso especifico do contexto Brasileiro, foi a compreensão dos problemas principais que o Brasil põe para pequenas-medias empresas estrangeiras, e algumas atenções que são necessárias em particular para empresários italianos. Para evoluir no tema, depois de essa introdução ao tema e ao contexto, o tópico mais importante é discutido. Então a investigação de esse trabalho se focaliza sobre o tema de como a GM Venture tem capacidade de responder com uma solução para os problemas presentados criando valor, e como ela criou um modelo de business que seja capaz de levar ao sucesso as empresas clientes no Brasil. A analise e conclusão do caso vão elaborar como a GM Venturo contribuiu ao resolver e evitar a complexidade Brasileira com meios legais e experiência, cooperando em positivo no crescimento da realidade econômica italiana em Brasil. Olhando ainda mais na analise da literatura e comparando o caso, parece evidente como o caso da GM Venture introduziu algumas novidades nos fatores já individuados, como uma ideais nova de network, e no mesmo tempo foi capaz de presentar fatores novos o subestimado, como a pronta velocidade e a confiança nos relacionamento de business que são muito importantes especialmente no Brasil.

The first definitive haematopoietic stem cells (HSCs) produced during embryonic development are generated from a specialised subset of endothelial cells known as haemogenic endothelium. Recently, it was reported that Gata3 plays a dual role in the development of sympathetic nervous system and haematopoietic system. In fact, Gata3 has proven to be crucial for the production of HSCs through regulation of catecholamine production from the co-developing sympathetic nervous system. Also, it was recently shown that Gata3 is expressed in the haemogenic endothelium and haematopoietic progenitor cells. Here, I will specifically examine the role of Gata3 in the production of HSCs; if it is expressed and plays a role in the precursors from which HSCs arise. Using a Gata3-GFP reporter mouse line, we found that Gata3 is expressed in various cell types in the HSCs microenvironment, including mesenchymal cells, endothelial cells, haematopoietic cells and sympathetic nervous system, and this expression was stage dependant. In the endothelial cells, we have found that the haemogenic endothelium activity is enriched in Gata3 expressing cells. Within the haematopoietic cells, we have found that Gata3 marks a specific stage along the developmental pathway towards the generation of definitive haematopoietic stem cells, and that Gata3 expressing haematopoietic cells are enriched for the most immature and stem cell like progenitors. Moreover, Gata3 will be specifically knocked out in haemogenic endothelial cells to determine whether it plays an essential role in the production of HSCs from the endothelium using the Vec-Cre system. We found that Gata3 within the haemogenic endothelium plays a major role in haematopoietic progenitors formation, and possibly haematopoietic stem cell formation. Finally, we used molecular assay (RNA seq) to identify the role of Gata3 in the haematopoietic stem cell microenvironment and found that Gata3 plays a major role in the development and differentiation of various cells and systems, and implicated Gata3 as cell cycle regulator. In summary, we found that Gata3 expressing cells is enriched for haemogenic endothelium, crucial for the haematopoietic progenitors formation, plays and important role in endothelial to haematopoietic transition, and plays a key developmental role in both haematopoietic stem cell and its microenvironment.

Uma fase polimérica inédita de polidimetilsiloxano/carvão ativado (PDMS-ACB) é proposta como fase extratora para extração por sorção em barras de agitação (SBSE). A barra de PDMS-ACB foi desenvolvida no laboratório usando um molde de teflon simples, demonstrando estabilidade e resistência aos solventes orgânicos. Utilizando uma única barra de PDMS-ACB mais de 150 extrações foram realizadas sem qualquer dano. A barra de SBSE contém aproximadamente 100 µL de revestimento polimérico, 2,36 mm de diâmetro e 2,2 cm de comprimento. A barra PDMS-ACB foi aplicada na extração de seis pesticidas (ametrina, atrazina, bifentrina, carbofurano, metribuzim e tebutiurom), com propriedades predominantemente polares, comumente aplicados em plantações de cana-de-açúcar. A barra PDMS-ACB foi utilizada na determinação de pesticidas em garapa através de cromatografia gasosa hifenada à espectrometria de massa (GC-MS). O planejamento experimental foi empregado na etapa de otimização da extração SBSE, um planejamento fatorial fracionado avaliou a influência dos principais parâmetros envolvidos. Posteriormente, o planejamento composto central (CCD) com conformação estrela foi explorado para otimizar os fatores significativos na extração. Apenas 200 µL de acetato de etila foram utilizados como solvente na dessorção líquida (LD). Para os compostos avaliados, o método apresentou limite de quantificação (LOQ) no intervalo de 0,5 - 40 µg L-1, as recuperações variaram entre 0,18 - 49,50 % e a precisão intra-dia variou de 0,072 - 8,40 %. Concluída a etapa de validação, o método foi aplicado em amostras reais de garapa disponíveis comercialmente em São Carlos-SP. / A novel polydimethylsiloxane/activated carbon (PDMS-ACB) is proposed as new polymeric phases for stir bar sorptive extraction (SBSE). The PDMS-ACB was developed in lab using simple teflon\'s mold, demonstrating remarkable stability and resistance to organic solvents, over 150 extractions without any damage. SBSE bar contained 100 µL of polymeric coating, a diameter of 2.36 mm and a length of 2.2 cm. It was applied to the determination of pesticides (ametryn, atrazine, bifenthrin, carbofuran, metribuzine and tebuthiuron) having predominantly polar properties, applied in sugarcane crops. PDMS-ACB was employed in the determination of pesticides in sugarcane juice using gas chromatography coupled to mass spectrometry (GC-MS). Experimental design was employed in the optimization step, a fractional factorial evaluated the main parameters involved in the extraction procedure. Afterwards, central composite design (CCD) was exploited to optimize the significant factors on the extraction. About 200 µL of ethyl acetate were employed as solvent in the liquid desorption (LD). The method presented limit of quantification (LOQ) from 0.5 to 40 µg L-1, recoveries varied 0.18 - 49.50% and precision intra-day 0.072 - 8.40%. Hence, the method was applied to the analysis of real sugarcane juice samples commercially available in São Carlos-SP.

付記する学位プログラム名: グローバル生存学大学院連携プログラム / 京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第22850号 / 農博第2433号 / 新制||農||1082(附属図書館) / 学位論文||R2||N5310(農学部図書室) / 京都大学大学院農学研究科生物資源経済学専攻 / (主査)准教授 伊庭 治彦, 教授 秋津 元輝, 教授 辻村 英之 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

Speed, power and reliability of analogue integrated circuits (IC) exhibit temperature dependency through the modulation of one or several of the following variables: band gap energy of the semiconductor, mobility, carrier diffusion, current density, threshold voltage, interconnect resistance, and variability in passive components. Some of the adverse effects of temperature variations are observed in current and voltage reference circuits, and frequency drift in oscillators. Thermal instability of a voltage-controlled oscillator (VCO) is a critical design factor for radio frequency ICs, such as transceiver circuits in communication networks, data link protocols, medical wireless sensor networks and microelectromechanical resonators. For example, frequency drift in a transceiver system results in severe inter-symbol interference in a digital communications system. Minimum transconductance required to sustain oscillation is specified by Barkhausen’s stability criterion. However it is common practice to design oscillators with much more transconductance enabling self-startup. As temperature is increased, several of the variables mentioned induce additional transconductance to the oscillator. This in turn translates to a negative frequency drift. Conventional approaches in temperature compensation involve temperature-insensitive biasing proportional-to-absolute temperature, modifying the control voltage terminal of the VCO using an appropriately generated voltage. Improved frequency stability is reported when compensation voltage closely follows the frequency drift profile of the VCO. However, several published articles link the close association between oscillation amplitude and oscillation frequency. To the knowledge of this author, few published journal articles have focused on amplitude control techniques to reduce frequency drift. This dissertation focuses on reducing the frequency drift resulting from temperature variations based on amplitude control. A corresponding hypothesis is formulated, where the research outcome proposes improved frequency stability in response to temperature variations. In order to validate this principle, a temperature compensated VCO is designed in schematic and in layout, verified using a simulation program with integrated circuit emphasis tool using the corresponding process design kit provided by the foundry, and prototyped using standard complementary metal oxide semiconductor technology. Periodic steady state (PSS) analysis is performed using the open loop VCO with temperature as the parametric variable in five equal intervals from 0 – 125 °C. A consistent negative frequency shift is observed in every temperature interval (≈ 11 MHz), with an overall frequency drift of 57 MHz. However similar PSS analysis performed using a VCO in the temperature stabilised loop demonstrates a reduced negative frequency drift of 3.8 MHz in the first temperature interval. During the remaining temperature intervals the closed loop action of the amplitude control loop overcompensates for the negative frequency drift, resulting in an overall frequency spread of 4.8 MHz. The negative frequency drift in the first temperature interval of 0 to 25 °C is due to the fact that amplitude control is not fully effective, as the oscillation amplitude is still building up. Using the temperature stabilised loop, the overall frequency stability has improved to 16 parts per million (ppm)/°C from an uncompensated value of 189 ppm/°C. The results obtained are critically evaluated and conclusions are drawn. Temperature stabilised VCOs are applicable in applications or technologies such as high speed-universal serial bus, serial advanced technology attachment where frequency stability requirements are less stringent. The implications of this study for the existing body of knowledge are that better temperature compensation can be obtained if any of the conventional compensation schemes is preceded by amplitude control. / Dissertation (MEng)--University of Pretoria, 2013. / Electrical, Electronic and Computer Engineering / unrestricted

This paper presents a comparative study on two Tabletop Roleplaying Games, Dungeons & Dragons and Blades in the Dark. This paper takes a look at the narrative differences within the two systems. More specifically investigate if Blades in the Dark is more playerdriven than Dungeons & Dragons. The two tested as closely as possible and will be compared with each other. This is a close reading on the rules and player agency rather than a one-on-one comparison. The comparisons were made regarding the mechanics and narrative differences within the systems rather than quality of the story. This study was conducted by running two sessions, one for each system, played by separate groups with the same game scenario and premise. Meaning that the background for both games, character and plot for the session were the same. Comparisons were made by observing player decisions, situations that arose and the influence that the gamemasters had on the game. It was found that there is a clear difference between the two systems, this difference regarding whether the players were reacting to the gamemaster, or the other way round. In Dungeons & Dragons, it was observed that the players reacted and acted according to what the gamemaster explained and played out, whereas in Blades in the Dark it appeared to be the opposite. Here we found that it was the Gamemaster who was reactive to the players instead. The paper concluded that Blades in the Dark is the more player-driven system. This short study could later be built upon and used by game developers to keep in mind and plan their future game systems around. Taking these observations on emergent narrative in Tabletop Role-playing Games into account can be used to create more player-centric and player-driven games. Meaning that the players are more in influence and decisions to make in the story and game. / I detta papper presenteras en jämförande undersökning av två rollspelssystem, Dungeons & Dragons och Blades in the Dark. Det här papperet kollar på skillnaderna när det kommer till spelar-drivet berättande inom de två spelen. Mer specifikt så utreds om Blades in the Dark är mer spelar-drivet än Dungeons & Dragons. De två kommer att testas så likt som möjligt och jämföras med varandra. Detta är en noggrann läsning av reglerna och spelarnas påverkan på spelets berättande, där vad som undersöks är spelets funktioner och skillnader i hur de berättas och presenteras, snarare än kvalitén på spelen eller berättelserna. Denna studie genomfördes genom att hålla två sessioner, en för varje system, spelad av separata grupper men med samma scenario och förutsättningar. Jämförelser gjordes genom att observera spelarbeslut, situationer som uppstod, samt det inflytande som spelmästare hade på spelet. En tydlig skillnad blev synlig mellan de två systemen var gällande huruvida spelarna reagerade på spelmästaren eller tvärtom. I Dungeons & Dragons observerades att spelarna reagerade och agerade enligt vad spelmästaren förklarade och spelade ut. I Blades in the Dark studerades en motsatt effekt, här upptäcktes att det var spelmästaren som reagerade på spelarna istället. Med denna undersökning kom vi fram till att Blades in the Dark är det mer spelar-drivna systemet. Den här studien kan i framtiden användas och byggas på av spelutvecklare för att göra nya spelsystem, med dessa observationer som framkom, kan komma till användning för att göra fler spel med mer spelar-drivet fokus. Vilket innebär att spelaren har mer inflytande på historiens som berättas och spelet i sig.