Magistrantūros studijų baigiamajame darbe pateikiami veiksnių, lemiančių pluoštinių linų morfogenezę in vitro, tyrimų duomenys. Darbo objektas – keturios pluoštinių linų veislės 'Dangiai', 'Sartai', 'Snaigiai' ir 'Vaižgantas'. Darbo metodai. Pluoštinių linų morfogenezei somatinių audinių kultūroje tirti naudoti hipokotilio eksplantai. Eksplantai auginti MS terpėje su skirtingais augimo reguliatorių kiekiais auginimo kambaryje. Vertintas pridėtinių pumpurų formavimosi dažnis (%) ir pridėtinių ūglių kiekis iš eksplanto (vnt.). Darbo rezultatai. Egzogeninių augimo reguliatorių priedas indukcijos terpėje daugeliu atvejų skatino tirtų pluoštinių linų veislių ūglių regeneraciją, tačiau optimali šio junginio koncentracija priklausė nuo genotipo. Iš tirtų pluoštinių linų veislių didžiausia morfogenine galia pasižymėjo veislės 'Vaižgantas' izoliuoti hipokotiliai. Priklausomai nuo genotipo ir maitinamosios terpės sudėties pluoštinių linų pridėtinių pumpurų formavimosi procesas hipokotilių kultūroje vyko 2,87-94,50 % dažniu regeneruojant 1,67-6,00 ūglius iš eksplanto. Efektyviam veislės 'Dangiai' ūglių regeneravimui maitinamąją terpę tikslinga papildyti 2,0 mg l-1 BAP + 0,1 mg l-1 NAR bei eksplantus paveikti 4 °C temperatūra 24 valandas. Veislės 'Sartai' izoliuotų hipokotilių audinių antrinę diferenciaciją skatino 72 valandų 7 °C temperatūros poveikis bei citokinino 1,0 mg l-1 2iP priedas maitinamojoje terpėje. Intensyviausiai veislės 'Snaigiai' eksplantai pumpurus formavo maitinamojoje... [toliau žr. visą tekstą] / The master work presents the results of fibre flax morphogenesis in vitro affecting factors. Object of the research − four fibre flax cultivars 'Dangiai', 'Sartai', 'Snaigiai' and 'Vaižgantas'. Research methods. To study fiber flax morphogenesis of somatic tissues hypocotyls were used as explant. Explants were placed on MS medium supplemented with different combinations of growth regulators and cultivated in growth chamber. The percentage of bud regeneration and the number of shoots per explant were evaluated. Research results. Exogenous growth regulators concentration in induction media increased fiber flax shoots regeneration, and therefore specific combination of growth regulators must be designed for each genotype. Among investigated genotypes, the hypocotyls of cultivars 'Vaižgantas' manifested the best morphogenic capability. Depending on genotype and medium composition fiber flax adventitious buds regeneration frequency varied from 2.87-94.50% and the number of shoots per explant reached 1.67-6.0, respectively. Hypocotyls of cultivar 'Dangiai' showed the best response on medium supplemented by 2.0 mg l-1 BAP with 0.1 mg l-1 NAA and pretreated by 4 °C temperature 24 hours. In the presence of only cytokinins, the highest mean value of regenerated shoots was observed on medium supplemented with 1.0 mg l-1 2iP and 72 hours 7 °C temperature pretreatment for hypocotyls of 'Sartai'. Growth regulators combination 2.0 mg l-1 2iP with 0.1 mg l-1 NAA and treatment by 4 °C... [to full text]

Magistrantūros studijų baigiamajame darbe pateikiami sėjamosios kanapės eksplanto tipo ir maitinamosios terpės sudėties poveikio mofogenezei in vitro tyrimų rezultatai. Darbo objektas – sėjamosios kanapės stiebo ir lapkočio segmentai. Darbo metodai: Sėjamosios kanapės eksplantai auginti MS, B5 ir White maitinamosiose terpėse, papildytose įvairiais augimo reguliatorių deriniais. Vertintas kaliaus susiformavimo dažnis (%), ūglių susiformavimo dažnis (%) ir šaknų susiformavimo dažnis (%). Darbo rezultatai. Sėjamosios kanapės morfogenezės procesas didžiąja dalimi priklauso nuo bazinės maitinamosios terpės sudėties. Izoliuoti stiebo segmentai intensyviausiai kalių indukavo bazinėje B5 maitinamojoje terpėje, o lapkočio segmentai − B5 maitinamojoje terpėje, papildytoje 1,0 mg l-1 TDZ + 0,5 mg l-1 NAR arba 1,0 mg l-1 KIN + 0,5 mg l-1 NAR. Pridėtinių ūglių formavimasis intensyviausiai vyko bazinėje B5 maitinamojoje terpėje, papildytoje 1,0 mg l-1 TDZ + 0,5 mg l-1 NAR (iš stiebo segmento audinių) ir 1,0 mg l-1 KIN + 0,5 mg l-1 NAR (iš lapkočio audinių). Izoliuoti stiebo segmentai didžiausiu dažniu šaknis formavo B5 maitinamojoje terpėje, papildytoje 1,0 mg l-1 KIN + 0,5 mg l-1 NAR, o lapkočio segmentai − 1,0 mg l-1 TDZ + 0,5 mg l-1 NAR. Tirti stiebo segmentai pasižymėjo didesne geba formuoti kalių, pridėtinius ūglius ir šaknis nei izoliuoti lapkočio segmentai. / The master work presents the effect of explants type and medium composition on morphogenesis in vitro of hemp. Object of the research – stem segments and leafstalk of hemp. Research methods. To study hemp morphogenesis in vitro hypocotyls and leafstalk were used as explants. Explants were placed on MS, B5 and White media supplemented with different combinations of growth regulators. The percentage of callus formation, shoot induction and root formation were evaluated. Research results. The current study indicates that there is strong medium composition effect on morphogenesis in vitro of hemp. From tested media most intensive callus genesis was observed on B5 medium, but from leafstalk – on B5 medium with 1.0 mg l-1 TDZ + 0.5 mg l-1 NAA or 1.0 mg l-1 KIN + 0.5 mg l-1 NAA. The growth regulators combination 1.0 mg l-1 TDZ + 0.5 mg l-1 NAA on B5 media was the most suitable for stem segments shoots induction while growth regulators combination 1.0 mg l-1 KIN + 0.5 mg l-1 NAA promoted the highest frequency of shoots induction from leafstalk segments. The highest root formation frequency was observed on the same basal medium supplemented with 1.0 mg l-1 KIN + 0.5 mg l-1 NAA from stem segments, but from leafstalk – on B5 medium with 1.0 mg l-1 TDZ + 0.5 mg l-1 NAA. Generally, the stem segments exhibited a higher morphogenic response than leafstalk of hemp.

Magistrantūros studijų baigiamajame darbe pateikiama šilokų pridėtinių ūglių regeneracija lapų segmentų kultūroje, priklausomai nuo genotipo ir augimo reguliatorių maitinamojoje terpėje. Darbo objektas – Sedum rupestre ‛Angeline’, Sedum reflexum ‛Cristatum’, Sedum fosterianum ‛Elegans’, Sedum maximum ‛Autumn Joy’ lapų audiniai. Darbo metodai: izoliuoti eksplantai auginti Murashige ir Skoog (MS) maitinamojoje terpėje su skirtingais augimo reguliatorių kiekiais. Susiformavę pridėtiniai ūgliai įšaknydinti MS maitinamojoje terpėje su skirtingais augimo reguliatorių kiekiais. Vertintas ūglių susiformavimo dažnis (%), ūglių kiekis iš eksplanto (vnt.) ir įsišaknijimo dažnis (%). Darbo rezultatai. Veislių ‛Elegans’ ir ‛Cristatum’ ūglių susiformavimo dažnį labiausiai skatino 0,75 mg l-1 BAP. Veislės ‛Angeline’ didžiausias ūglių susiformavimo dažnis nustatytas terpėje, papildytoje 3,0 mg l-1 BAP. Veislės ‛Autum joy’ izoliuoti lapo segmentai 100 % dažniu ūglius formavo terpėse su 3,0 mg l-1 BAP + 0,5 mg l-1 NAR ir 4,0 mg l-1 BAP + 0,5 mg l-1 NAR. Veislės ‛Elegans’ didžiausias ūglių kiekis eksplantui gautas terpėje papildytoje 0,75 mg l-1 BAP. Veislės ‛Angeline’ daugiausia ūglių iš eksplanto susiformavo terpėse su 0,75 mg l-1 BAP ir 3,0 mg l-1 BAP. Terpėje, papildytoje 3,0 mg l-1 BAP gautas didžiausias veislės ‛Cristatum’ ūglių kiekis vienam eksplantui. Veislės ‛Autum joy’ eksplantai daugiausia ūglių suformavo terpėse, papildytose 3,0 mg l-1 BAP + 0,5 mg l-1 NAR ir 4,0 mg l-1 BAP + 0,5... [toliau žr. visą tekstą] / The master work presents orpine adventitious shoots regeneration in leaves segments cultures, depending on genotype and growth regulators in the culture medium. Object of the work – Sedum rupestre ‛Angeline’, Sedum reflexum ‛Cristatum’, Sedum fosterianum ‛Elegans’, Sedum maximum ‛Autumn Joy’ leaves tissues. Method of the work – isolated explants were growing on Murashige ir Skoog (MS) nutrition medium with different content of growth regulators. Adventitious shoots were transferred to MS culture medium for rhizogenesis induction. Shoots formation frequency, number of shoots per explant and rooting frequency were evaluated. The results of work. The highest shoots formation frequency of orpine varieties ‛Elegans’ and ‛Cristatum’ has been obtained under influence of 0.75 mg l-1 BAP. The 3.0 mg l-1 BAP concentration in culture medium resulted in highest shoots regeneration frequency of variety ‛Angeline’. All isolated explants of ‛Autum joy’ formed adventitious shoots in the medium supplemented with 3.0 mg l-1 BAP + 0.5 mg l-1 NAA and 4.0 mg l-1 BAP + 0.5 mg l-1 NAA. While 0.75 mg l-1 BAP in culture medium stimulated shoots formation frequency of variety ‛Elegans’. The highest shoots number per explant of variety ‛Angeline’ has been establishing in medium with 0.75 mg l-1 BAP and 3.0 mg l-1 BAP. Medium supplemented with 3.0 mg l-1 BAP resulted in highest shoots number per explant of ‛Cristatum’. Explants of variety ‛Autum joy’ highest shoots number per explant formed in medium... [to full text]

Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 130-148). Also available on the Internet.

O Pinus elliotti representa o principal produtor atual de exploração de resina no setor florestal brasileiro. Recentemente, plantios de P. elliottii X P. caribaea vêm se destacando, principalmente, pela sua maior produtividade em biomassa. Além disto, a resina do híbrido apresenta características químicas semelhantes aos seus parentais. Porém, poucas são as informações quanto a estaquia de espécies do gênero Pinus, e se focarmos na propagação de árvores adultas, tal carência é ainda maior, principalmente ao se considerar a dificuldade de rejuvenescimento/revigoramento do material genético. Neste contexto, o presente trabalho teve como objetivo geral avaliar o enraizamento adventício do P. elliottii var. elliottii e P. elliottii X P. caribaea adultos. Para tanto, o trabalho foi dividido em três diferentes estudos. O primeiro (Capítulo 2) avaliou o enraizamento adventício de estacas das matrizes adultas de P. elliottii e P. elliottii X P. caribaea no 5° estágio de enxertia seriada com e sem podas sucessivas, sob ação do AIB, talco com fungicida Cerconil® e terebentina. O segundo (Capítulo 3) foi baseado na avaliação do enraizamento adventício de estacas no 2° estágio de enxertia seriada de uma matriz adulta de P. elliottii var. elliottii submetida a um ano de podas sucessivas, sob ação do AIB, terebentina e paclobutrazol. Por sua vez, o terceiro estudo (Capítulo 4) consistiu na avaliação do enraizamento adventício de braquiblastos e brotos de duas matrizes adulta de P. elliottii e P. elliottii X Pinus caribaea, sob ação do AIB e paclobutrazol. Os resultados permitiram inferir que a sobrevivência e o enraizamento adventício aos diferentes tratamentos variaram de acordo com o genótipo e com as características morfológicas das estacas (grau de lignificação e tipo de estacas, brotos e braquiblastos). De maneira geral, os resultados evidenciaram a atuação das enxertias seriadas e das podas sucessivas na restauração da competência rizogênica das estacas dos brotos das matrizes adultas de P. elliottii e P. elliottii X P. caribaea, promovido pelo rejuvenescimento/ revigoramento parcial das matrizes. Além disto, a estaquia de braquiblastos mostrou ser uma ferramenta promissora para a propagação de espécies do gênero Pinus. / Pinus elliottii is the main current resin extraction producer in the Brazilian forest sector. Recently, plantations of P. elliottii X P. caribaea play a great importance in the production systems mainly by its high biomass productivity. In addition, the hybrid resin has chemical characteristics similar to their parents. However, there is little information on obtaining clones, and considering the propagation of mature trees, this lack of information is even greater, mainly concerning the difficulty to achieve the rejuvenation/ reinvigoration of the genetic material. Based on theses information, the present work was aimed to evaluate the adventitious rooting of mature P. elliottii var. elliottii and P. elliottii X P. caribaea cuttings. Therefore, the work was divided into three basic studies. The first study (Chapter 2) was based on the evaluation of adventitious rooting of mature P. elliottii and P. elliottii X caribaea cuttings of grafts up five sequential grafting with and without pruning, under the action of AIB, talc with fungicide and turpentine. The second (Chapter 3) was based on the evaluation of adventitious rooting of mature P. elliottii cuttings of grafts up two sequential grafting with pruning, under the action of IBA, paclobutrazol and turpentine. The third (Chapter 4) evaluated the adventitious rooting of brachyblast and shoots of mature P. elliottii and P. elliottii X caribaea cuttings, under the action of IBA and paclobutrazol. Results showed that the cutting survival and root growth, under the action of different treatments, varied according to the genotype and morphological characteristics of the material (cutting lignifications and type, brachyblast or shoot). Overall, our results showed the performance of serial grafting and pruning on the restoration of rooting competence in P. elliottii and P. elliottii X P. caribaea stem cuttings, promoted by the rejuvenation of trees. Moreover, the cutting of brachyblast can be a promising tool for the propagation of Pinus species.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-06-07T22:50:41Z No. of bitstreams: 2 kennedy_paiva_porfirio.pdf: 1795601 bytes, checksum: b5586d73e2887546c047ef31257c28b7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-06-22T15:23:55Z (GMT) No. of bitstreams: 2 kennedy_paiva_porfirio.pdf: 1795601 bytes, checksum: b5586d73e2887546c047ef31257c28b7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-06-22T15:23:55Z (GMT). No. of bitstreams: 2 kennedy_paiva_porfirio.pdf: 1795601 bytes, checksum: b5586d73e2887546c047ef31257c28b7 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Este trabalho teve como objetivo desenvolver procedimentos de germina??o, estaquia e micropropaga??o de Xylopia aromatica. Os experimentos foram conduzidos na Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), em diamantina ? MG, cujos trabalhos foram divididos em dois cap?tulos. No primeiro cap?tulo foram realizados oito experimentos. A germina??o foi avaliada em quatro experimentos, onde sementes de Xylopia aromatica, separadas em lotes distintos quanto ? densidade, foram submetidas ? quebra de dorm?ncia utilizando GA3 em diferentes concentra??es (0; 25; 50; 100; 250; 500; e 1000 mg L-1), nos tempos de imers?o 24 e 48 horas. N?o ocorreu germina??o durante os 210 dias de avalia??o. Foram realizados quatro experimentos de estaquia, onde segmentos caulinares (com e sem folhas) e radiculares com classes de di?metros distintas, foram imersos por 30 segundos em solu??o de AIB (0; 2000; 4000; 6000; 8000 e 10.000 mg L-1) a fim de induzir o enraizamento advent?cio. Foi avaliado o percentual de enraizamento durante 140 dias. N?o houve enraizamento em nenhum dos experimentos, porem ocorreu brota??es nas estacas caulinares que foram imersas nas concentra??es de 2000, 4000 e 6000 mg L-1 de AIB. No segundo cap?tulo, foram realizados seis experimentos, que envolveram etapas de multiplica??o, alongamento e enraizamento in vitro. Explantes foram submetidos a diferentes meios de cultura (MS e WPM), e concentra??es de BAP (0,5 e 0,8 mg L-1), objetivando determinar o melhor meio de cultura e concentra??o de BAP para a multiplica??o da esp?cie. Avaliou-se tamb?m, o alongamento em explantes com o uso de combina??es de ANA e BAP e GA3, e enraizamento com o uso de AIB e ANA. O meio MS acrescido de 0,8 mg L-1 de BAP foi o que apresentou melhores resultados para a multiplica??o de Xylopia aromatica. Na fase de alongamento, o GA3 na concentra??o de 5,0 mg L-1 foi o regulador de crescimento que apresentou melhor resultado em altura e n?mero de folhas. No enraizamento, o AIB e o ANA n?o foram eficazes na indu??o de ra?zes, necessitando mais estudos relacionados ? etapa de enraizamento para a esp?cie. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / This study aimed to develop germination procedures, cutting and micropropagation Xylopia aromatica. The experiments were conducted in Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) in Diamantina - MG, whose works were divided into two chapters. In the first chapter were carried out eight experiments. Germination was evaluated in four experiments where aromatica Xylopia seeds, separated into separate lots for density, were submitted to dormancy breaking using GA3 at different concentrations (0, 25, 50, 100, 250, 500, and 1000 mg L-1) under immersion for 24 and 48 hours. Not germinated during the 210 days of evaluation. Four cutting experiments were carried out where segments shoot (with and without leaves) and root with different diameter classes were immersed for 30 seconds in AIB solution (0; 2000; 4000; 6000; 8000 and 10,000 mg L-1) to induce adventitious roots. Rooting percentage was evaluated during 140 days. There was no rooting experiments, however sprouting occurred in the cuttings were dipped in concentrations of 2000, 4000 and 6000 mg L-1 AIB. In the second chapter, we were conducted six experiments, involving multiplication steps, stretching and in vitro rooting. Explants were subjected to different culture medium (MS and WPM) and BAP (0,5and 0,8mg L-1), in order to determine the best medium and concentration of BAP for the multiplication of the species. It also evaluated the elongation explants using combinations of ANA and BAP and GA3 and rooting using AIB and ANA. The MS medium plus 0.8 mg L-1 BAP showed the best results for the multiplication of Xylopia aromatica. In the stretching step, the concentration of GA3 at 5,0 mg L-1 was the growth regulator showed better results in height and leaf number. Rooting, AIB and ANA were not effective in inducing roots, requiring more studies related to the rooting stage for the species.

The work is focused on spring application of plant growth regulators and the operating conditions of agricultural society Kosova Hora, as Preparations were applied during the growth phase stands 32-33 BBCH scale, with 2 types of varieties - a line of hybrid canola varieties. 3 preparations were evaluated (Galleko, Toprex, Caryx) and one untreated area for control of the comparison of products. During the vegetation period were gradually on individual plots monitored the main elements of the plant yield (number of plants, number of pods, HTS, the number of branches). After harvesting canola yield was determined at the experimental plot. Results showed a positive reaction to stand on the treated area CARYX product. Attainment and income (4, 53 t / ha) on the experimental plot was achieved on a plot of hybrid rape. Difference treated variants compared to the control side were 0, 28 t / ha. This result is not statistically provable. The results are largely influenced the course of weather throughout the growing season. For better statistical power experiment would be preferable to multi attempts.

Made available in DSpace on 2014-06-11T19:32:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-06-19Bitstream added on 2014-06-13T19:22:05Z : No. of bitstreams: 1 camili_ec_dr_botfca.pdf: 1038671 bytes, checksum: 1a53c411e9d03968dbf9c78cdb7abc49 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O Brasil possui vantagens competitivas que, no caso da uva, se resumem na possibilidade de produção e exportação da fruta durante alguns meses, quase sem concorrência. Porém, alguns fatores têm limitado a expansão do cultivo para áreas tropicais, como por exemplo, a baixa produtividade de cultivares pouco adaptados. Assim, este projeto de pesquisa teve por objetivo estudar o efeito da aplicação de novos produtos visando uniformizar a brotação e aumentar a produtividade e a qualidade de bagas do cultivar de uva sem sementes Superior Seedless. Os experimentos foram conduzidos na Fazenda Koshiyama, localizada no município de Juazeiro/BA, no período de 15/05 a 21/09/2006. Com a finalidade de avaliar o efeito do biorregulador Stimulate® (0,009% de cinetina (citocinina), 0,005% de ácido giberélico (GA3), 0,005% de ácido indolilbutírico (IBA-auxina) e 99,981% de ingredientes inertes) e de X-Cyte® (0,04% de cinetina (citocinina) e 99,96% de ingredientes inertes) na uniformidade de brotação e dos biorreguladores Stimulate®, X-Cyte® e N-Large® (4% de ácido giberélico (GA3) e 96% de ingredientes inertes) no desenvolvimento das bagas, foram conduzidos três experimentos. Experimento 1: T1: Dormex® (5%); T2: Dormex® (5%) + Nitro Plus 9®; T3: Stimulate® (0,5%); T4: Stimulate® (0,5%) + Nitro Plus 9®; T5:Stimulate® (1,0%); T6: Stimulate® (1,0%) + Nitro Plus 9®; T7: X-Cyte® (0,25%); T8: XCyte ® (0,25%) + Nitro Plus 9®; T9: X-Cyte® (0,50%); T10: X-Cyte® (0,50%) + Nitro Plus 9®; T11: X-Cyte® (0,25%) + Stimulate® (0,5%) e; T12: X-Cyte® (0,25%) + Stimulate® (0,5%) + Nitro Plus 9®. Experimento 2: T1: Pro-Gibb®; T2: Stimulate® (Dose 1); T3: Stimulate® (Dose 2); T4: Stimulate® (Dose 3); T5: Pro-Gibb® + X-Cyte® (Dose Baixa - DB); T6: Pro-Gibb® + X-Cyte® (Dose Média - DM); T7: Pro-Gibb® + X-Cyte®... / Brazil has some competitive advantages in grape production, specially harvesting all-year-around in almost the whole of the country. Therefore, Brazilian fruits can be exported for a few months practically without international competition. However, some factors have been limiting grape growing expansion in tropical areas. For example, low yields of less adapted cultivars. The field trials of this work were performed between May 15th and September 21st 2006, at Koshiyama farm, in Juazeiro municipality (Bahia State / Northeastern Brazil). The main goal was to analyze the effect of new commercial products intended to uniformizar sprouting, increasing yields and berry quality for Superior Seedless grapes. It was evaluated the effect of the following bioregulators in sprouting uniformity: Stimulate® (0,009% de kinetin (cytokinin), 0,005% of gibberellic acid (GA3), 0,005% indolbutiric acid (IBA-auxin); 99,981% of inert ingredients); X-Cyte® (0,04% kinetin (cytokinin) and 99,96% of inert ingredients). Three field trials were also performed, to verify the effect of Stimulate®, X-Cyte® e N-Large® (4% gibberellic acid (GA3) and 96% inert ingredients) on berry development: Trial 1: T1: Dormex® (5%); T2: Dormex® (5%) + Nitro Plus 9®; T3: Stimulate® (0,5%); T4: Stimulate® (0,5%) + Nitro Plus 9®; T5: Stimulate® (1,0%); T6: Stimulate® (1,0%)+ Nitro Plus 9®; T7: X-Cyte® (0,25%); T8: X-Cyte® (0,25%) + Nitro Plus 9®; T9: X-Cyte® (0,50%); T10: X-Cyte® (0,50%) + Nitro Plus 9®; T11: X-Cyte® (0,25%) + Stimulate® (0,5%)e; T12: X-Cyte® (0,25%) + Stimulate® (0,5%) + Nitro Plus 9®. Trial 2: T1: Pro-Gibb®; T2: Stimulate® (Dose 1); T3: Stimulate® (Dose 2); T4: Stimulate® (Dose 3); T5: Pro-Gibb® + XCyte ® (Low Dose - DB); T6: Pro-Gibb® + X-Cyte® (Intermediate Dose- DM); T7: Pro-Gibb® + X-Cyte® (High Dose- DA); T8: N-Large®; T9: N-Large® + X-Cyte® (DB); T10: N-Large®... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:32:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-03Bitstream added on 2014-06-13T18:43:51Z : No. of bitstreams: 1 merlin_tpa_dr_botfca.pdf: 564602 bytes, checksum: 0d5aeeb10e9efed41f754174a7c6205d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O presente trabalho objetivou avaliar a aplicação de reguladores vegetais (tratamento de semente e via foliar) em limoeiro ´Cravo´ (Citrus limonia Osbeck) visando o encurtamento do tempo de produção do porta-enxerto. O experimento foi dividido em duas etapas, sendo a primeira realizada no laboratório de Análise de Sementes, Departamento de Produção Vegetal - Agricultura da Faculdade de Ciências Agronômicas - Botucatu, SP, determinando-se a taxa de embebição das sementes. Após este procedimento, realizou-se o teste de germinação das sementes após tratamento com os produtos (testemunha, ácido giberélico GA3 10 mg 0,2 L-1, ácido giberélico GA3 20 mg 0,2 L-1, cinetina 10 mL 0,2 L-1, piraclostrobina + metil tiofanato 0,03 mL L-1, cinetina + ácido giberélico + ácido indolilbutírico 30 mL 0,2 L-1, ácido giberélico GA4+7 + 6-benziladenina 1 mL 0,2 L-1, ácido giberélico GA4+7 + 6-benziladenina 2,12 mL 0,2 L-1, cloreto de chlormequat 0,2 mL 0,2 L-1 e cloreto de chlormequat 0,2 mL 0,2 L- 1 + ácido giberélico GA3 20 mg 0,2 L-1. A segunda etapa do experimento foi realizada no viveiro ELPA Mudas Cítricas, localizado na cidade de Botucatu, SP. Após resultado da taxa de embebição as sementes foram mantidas em contato com os tratamentos e semeadas em tubetes com substrato de fibra de coco. Foram empregados dez tratamentos aplicados via foliar, mensalmente: testemunha, ácido giberélico GA3 50 mg L-1, ácido giberélico GA3 100 mg L-1, cinetina 50 mL L-1, piraclostrobina + metil tiofanato 0,18 mL L-1, cinetina + ácido giberélico + ácido indolilbutírico 150 mL L-1, ácido giberélico GA4+7 + 6-benziladenina 5 mL L-1, ácido giberélico GA4+7 + 6-benziladenina 10 mL L-1, cloreto de chlormequat... / The aim of this project was to evaluate the plant growth regulator application (seed and leaves) in rangpur lime (Citrus limonia Osbeck) on rootstock production in a shorter period of time. The experiment was divided into two steps; the first one was carried in the Seed Analysis Laboratory, Crop Science Department - College of Agricultural Sciences - Botucatu, SP, in order to analyze the inhibition. Afterwards, the germination test was done after the contact of the seeds with the treatments (control, gibberellic acid GA3 (10 mg L-1) 10 mg 0,2 L-1, gibberellic acid GA3 (10 mg L-1) 20 mg 0,2 L-1, kinetin (0,4 mg L-1) 10 mL 0,2 L-1, pyraclostrobin + thiophanate methyl (50 g L-1 + 450 g L-1) 0,03 mL L-1, kinetin (90 mg L-1) + gibberellic acid (50 mg L-1) + indolebutyric acid (50 mg L-1) 30 mL 0,2 L-1, gibberellic acid GA4+7 (19 mg L-1) + 6-benzyladenine (19 mg L-1) 1 mL 0,2 L-1, gibberellic acid GA4+7 (19 mg L-1) + 6-benzyladenine (19 mg L-1%) 2,12 mL 0,2 L-1, chlormequat chloride (100 g L-1) 0,2 mL 0,2 L-1 and chlormequat chloride (100 g L-1) 0,2 mL 0,2 L-1 + gibberellic acid GA3 (10 mg L-1) 20 mg 0,2 L-1. The second part of the experiment was carried out in ELPA Citric Seedlings’ Nursery, in Botucatu, SP. After the inhibition test, the seeds were kept in contact with the treatments and sowed in the coconut fiber substrate. Ten treatments were provided monthly by foliar application: control, gibberellic acid GA3... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:28:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-18Bitstream added on 2014-06-13T18:57:49Z : No. of bitstreams: 1 depietro_j_me_jabo.pdf: 1424480 bytes, checksum: fd0d58d73de3ddb09b7b417a5389737f (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Face à carência de estudos relacionados à fisiologia pós-colheita de flores, a presente pesquisa teve como finalidade estudar a fisiologia e conservação póscolheita de flores cortadas de rosas cv. Vega, considerando os fatores associados à senescência floral e perda de vida decorativa. Os experimentos foram conduzidos em delineamento inteiramente casualizado, em esquema fatorial. Em todos os experimentos, as rosas foram mantidas em ambiente de laboratório com 20±2ºC e 67±3% UR, padronizadas em 50 cm e realizadas as seguintes avaliações (exceto para o experimento 5): perda de massa fresca, massa seca, taxa respiratória, notas de qualidade (escurecimento, turgescência e curvatura), conteúdo relativo de água, carboidratos solúveis e redutores, antocianina e coloração. Para o experimento 5 foi avaliada a quantidade de água absorvida e transpirada das rosas. No primeiro experimento, as flores foram colocadas dentro de uma câmara hermética e expostas ao 1-MCP, por um período de seis horas, nas seguintes concentrações: 1) Água destilada (controle); 2) 1-MCP 100 a ppb; 3) 1-MCP a 250 ppb; 4) 1-MCP a 500 ppb. Ao contrário do tratamento com água destilada, todas as concentrações de 1-MCP foram eficientes para retardar a senescência das flores, com destaque para a de 500 ppb que melhor manteve a qualidade, além de prolongar a vida de vaso das rosas até 19 dias. No segundo experimento, as flores permaneceram nas seguintes soluções de manutenção: 1) Água destilada (Controle); 2) 8-HQC (200 mg.L-1); 3) Sacarose (2%) + 8-HQC (200 mg.L-1); 4) Ácido Cítrico (75 mg.L-1); 5) Sacarose (2%) + Ácido Cítrico (75 mg.L-1); 6) 6- BA (60 mg.L-1); 7) Sacarose (2%) + 6-BA (60 mg.L-1). As rosas foram muito sensíveis à 6-benziladenina, associada ou não à sacarose, e perderam a qualidade aos seis dias de vida de vaso. Em contrapartida, o tratamento com 8-hidroxiquinolina... / Given the lack of studies on postharvest physiology of flowers, this research aims to study the postharvest physiology and keeping quality of Vega cut rose, were observed the factors associated with floral senescence and loss of decorative life. The experiments followed complete randomized design, in factorial arrangement. In all experiments, the roses were kept at room temperature with 20 ± 2ºC and 67±3% UR, standardized at 50 cm and it were analysis (except the fifth experiment): weight loss, dry weight, rate respiratory, quality (browning, turgidity and curvature), relative water content, soluble and reducing carbohydrates, anthocyanin, color and longevity. On the fifth experiment was to evaluation the water absorbed and transpired roses. On the first experiment, the flowers were placed inside an airtight chamber and exposed to 1-MCP for a period of six hours, in these concentrations: 1) Distilled water (control); 2) 1-MCP (100 ppb); 3) 1-MCP ( 250 ppb); 4) 1-MCP (500 ppb). Unlike treatment with distilled water, all concentrations of 1-MCP were effective in delaying the senescence of flowers, however, the concentration of 500 ppb of 1-MCP induced better maintenance of quality and extended vase life of roses to 19 days. On the second experiment, the flowers remained in these following holding solutions: 1) Distilled water (Control); 2) 8-HQC (200 mg.L-1); 3) Sucrose (2%) + 8-HQC (200 mg.L-1); 4) Citric Acid (75 mg.L-1); 5) Sucrose (2%) + Citric Acid (75 mg.L-1); 6) 6-BA (60 mg.L-1); 7) Sucrose (2%) + 6-BA (60 mg.L-1). The roses were very sensitive to 6-benzyladenine, with or without sucrose, and lost quality of six days of vase life. In contrast, treatment with 8-hydroxyquinoline has proved the most promising to maintain the quality of the flowers, for ten days. On the third experiment, the roses were treated this way: 1) Distilled water (Control), 2) STS (1 mM) ... (Complete abstract click electronic access below)