Thesis (MScSportSc)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: There are extensive literature on the PEH response after acute and chronic aerobic and resistance exercise, as well as a few studies on concurrent and water exercise. However, there is comparatively little evidence that high intensity interval training (HIIT) elicits similar post exercise blood pressure reductions (PEH) compared to other types of exercise. Furthermore, it is difficult to quantify the magnitude of the hypotensive response following these exercises, due to variations in exercise protocols in terms of intensity and duration. Both these training variables are considered important determinants of the magnitude and duration of the PEH response. The current study determined the magnitude of the PEH response after an acute bout and six sessions of HIIT, and the effects after two weeks of detraining in overweight/obese young women. Twenty young women (aged 21 ± 2 years) volunteered for the study. All the subjects were normotensive (SBP: 119.2 ± 5.6 mmHg and DBP: 78.8 ± 4.1 mmHg). Subjects performed six sessions of HIIT within two weeks and detrained for two weeks. SBP, DBP, MAP and HR were monitored during seated recovery after exercise for 60 min to determine the change from resting values. The overall outcome showed that an acute HIIT session resulted in a reduction of 2.9 mmHg in SBP which approached near clinical significance, while six sessions of HIIT caused a clinically significant reduction of 5.3 mmHg; this response was almost totally reversed after detraining. There were no clinically significant reductions in DBP after the acute or six sessions of HIIT (1.7 and 2.7 mmHg, respectively). However, a clinically significant hypotensive response of 3.9 mmHg was sustained after detraining following the maximal exercise capacity test. MAP also reduced by a magnitude of 2.3 and 5.6 mmHg, respectively, after the acute bout and six sessions of HIIT, and detraining values were still 2.9 mmHg lower than resting values and approached near clinical significance. The results indicate that both an acute bout and six sessions of HIIT elicited a meaningful PEH response. However, the six sessions of HIIT caused a clinically significant reduction which was approximately twice the acute session. Likewise, detraining showed clinically significant effects in DBP and MAP, but SBP returned to near baseline values. This suggests that in only two weeks, the accumulated effects of six sessions of HIIT elicited a greater hypotensive response than after an acute session of HIIT. / AFRIKAANSE OPSOMMING: Daar is omvattende literatuur oor die post-oefening hipotensie (POH) na afloop van akute en kroniese aërobiese en weerstandsoefeninge, asook enkele studies oor gelyktydige krag- en uithouvermoë- en wateroefeninge. Daar is egter relatief min bewyse dat hoë intensiteit interval oefening (HIIO) soortgelyke post-oefening afnames in bloeddruk (POH) in vergelyking met ander tipes oefening veroorsaak. Voorts is dit moeilik om die omvang van die hipotensiewe respons na afloop van oefening te kwantifiseer, hoofsaaklik as gevolg van die variasies in oefeningprotokolle in terme van intensiteit en tydsduur. Beide hierdie inoefeningveranderlikes word as belangrike determinante van die omvang en die tydsduur van die POH respons beskou. Die huidige studie het die omvang van die POH respons na ʼn akute sessie en ses sessies HIIO, en die gevolge na afloop van twee weke se nie-inoefening (“detraining”) by oorgewig/vetsugtige jong dames, bepaal. Twintig jong dames (ouderdom 21 ± 2 jaar) het vrywillig ingestem om aan die studie deel te neem. Al die deelnemers was normotensief (SBD: 119.2 ± 5.6 mmHg en DBD: 78.8 ± 4.1 mmHg). Die deelnemers het ses sessies HIIO binne twee weke voltooi en het daarna vir twee weke geen inoefeningsessies gehad nie. SBD, DBD, GAD en HS is tydens ʼn sittende herstelfase vir 60 minute gemonitor om die verandering vanaf rustende waardes te bepaal. Die algehele uitkoms toon dat ʼn akute HIIO sessie ʼn afname van 2.9 mmHg in SBD tot gevolg gehad het wat aan kliniese betekenisvolheid grens, terwyl ses sessies van HIIO ʼn klinies betekenisvolle afname van 5.3 mmHg veroorsaak het; hierdie respons wat bykans volledige omgekeerd na die twee weke met geen inoefening. DBD het geen kliniese betekenisvolle afname na afloop van die akute of ses sessies van HIIO getoon nie (1.7 en 2.7 mmHg, respektiewelik). ʼn Klinies betekenisvolle hipotensiewe respons van 3.9 mmHg is egter gevind na die geen inoefeningsperiodes. GAD het ook met ʼn omvang van 2.3 en 5.6 mmHg, respektiewelik, verminder na afloop van die akute sessie en ses sessies van HIIO. Die geen inoefening waardes was steeds 2.9 mmHg laer as die rustende waardes en het aan kliniese betekenisvolheid gegrens. Die resultate toon dat beide ʼn akute sessie en ses sessies van HIIO ʼn betekenisvolle POH respons ontlok het. Ses sessies van HIIO het egter ʼn klinies betekenisvolle afname, wat ongeveer twee keer soveel as die afname van die akute sessie was, veroorsaak. In dieselde lig het ʼn twee weke geen inoefeningsperiode steeds klinies betekenisvolle veranderinge in DBD en GAD getoon, maar SBD het tot naby aan die basislyn waardes teruggekeer. Hierdie resultate suggereer dat in slegs twee weke die geakkumuleerde effekte van ses sessies van HIIO ʼn groter hipotensiewe respons as na ʼn akute sessie van HIIO ontlok het.

Thesis (MScSportSc)-- Stellenbosch University, 2013. / ENGLISH ABSTRACT: There is a growing concern in South Africa and worldwide about the global epidemic of obesity and overweightness among the general population. Obesity mediates the pathogenesis of pathological conditions and is associated with a poor quality of life, high morbidity and mortality rates and a huge burden on an individual’s and the health system’s infrastructure and finances. The answer to this rising epidemic is weight loss. Endurance training has been shown to induce weight loss however, people usually cite lack of time as a barrier to meaningful participation in exercise programmes. High intensity interval training (HIIT) therefore emerges as a potential solution to these barriers as it takes a relatively short period of time compared to endurance training. Despite the differences in exercise durations the most cogent advantage is that HIIT elicits not just similar, but even superior central and peripheral adaptations. The central and peripheral adaptations have been shown to enhance weight loss, improve blood lipids and glucose levels, as well as decreasing blood pressure. The challenge facing exercise physiologists is to find the optimal exercise intensity and duration of HIIT bouts which would be time efficient, safe and well tolerated by overweight and obese people. The shortcomings of literature are that most HIIT studies have focused on healthy, overweight and obese men and these studies cannot be extrapolated to women who have been shown to respond differently to training. Moreover, other interventions investigating the effects of HIIT in women and men have been longer term rather than short term interventions. In order to fill the gaps in the literature, the main aim of this study was to investigate the training and detraining effects of a short-term HIIT programme on selected health-related measures in young overweight and obese women. To this end, a non-random sample of 20 overweight and obese women (aged 18-25) volunteered to participate in this study. Selected health-related outcomes were measured prior to training. The pre-training testing was followed by the HIIT intervention which was two weeks and consisted of six sessions using the 10 – 15x1 minute running at 90% HRmax which was separated by one minute active recovery periods at 50-60% of HRmax. The HIIT intervention was followed by a post test in which baseline measurements were repeated. This was then followed by a two week detraining period and follow up testing. The main finding of this study was that a period of two weeks of HIIT can elicit adaptations that can lower the risk profiles of young overweight and obese women. The results showed a statistically significant decrease in body mass (1.6%, p = 0.001), fat mass (3.7%, p = 0.001) and waist circumference (4.8%, p = 0.001), and an increase in lean mass of 1.9% (p = 0.001). There was also a decrease in blood glucose (11%, p = 0.001), total cholesterol (10.4 %, p = 0.01), systolic (3.4%, p = 0.001) and diastolic blood pressure (5.8%, p = 0.001) levels. Finally there was a statistically significant increase in relative VO2max and exercise capacity after the HIIT The follow-up testing after two weeks of detraining shows that the metabolic adaptations that were achieved by the HIIT protocol are relatively lasting or are at least not completely reversed. The weight loss induced by HIIT is important in that it is the major target in lowering the prevalence of overweightness and obesity. The HIIT protocol in this study emerges as a time efficient strategy in eliciting positive adaptations in clinical populations and healthy people. Moreover these findings suggest that 10 minute and 15 minute HIIT work bouts at near-maximal intensities are possibly the minimum amount of training that is needed to induce significant weight loss and other positive health-related outcomes. / AFRIKAANSE OPSOMMING: Daar bestaan ʼn toenemende besorgdheid in Suid-Afrika en wêreldwyd oor die globale epidemie van obesiteit en oorgewig onder die algemene bevolking. Obesiteit fasiliteer die patogenese van verskeie siektetoestande en word met ʼn swak kwaliteit lewe, hoë morbiditeit en mortaliteit en ʼn geweldige las op ʼn individu en die gesondheidsowerhede se infrastruktuur en finansies geassosieer. Een van die antwoorde op hierdie stygende epidemie is gewigsverlies. Dit is reeds gewys dat uithouvermoë oefening saam met ʼn kalorie beperkende dieet gewigsverlies in die hand werk. Mense dui egter ʼn tekort aan tyd as ʼn hindernis tot betekenisvolle deelname aan ʼn oefenprogram aan. Hoë intensiteit interval inoefening (HIIO) is dus ʼn potensiële oplossing tot hierdie hindernis aangesien dit in vergelyking met uithouvermoë inoefening in ʼn relatiewe korter periode van tyd uitgevoer kan word. Afgesien van die verskille in inoefenperiodes is die mees logiese voordeel dat die HIIO nie net soortgelyke nie, maar self beter sentrale en periferale fisiologiese aanpassing voortbring. Die sentrale en periferale aanpassing verhoog gewigsverlies, verbeter bloedlipiedes en glukose vlakke, en veroorsaak ʼn afname in bloeddruk. Alhoewel ʼn aantal studies die voordele van HIIO by jonger en ouer populasies aandui, is baie min studies op vrouens uitgevoer. Bevindinge kan nie noodwendig na vrouens ekstrapoleer word nie omdat hulle dikwels verskillend op inoefening as mans reageer. Dit is ook nie bekend of ʼn kort HIIO intervensie ʼn betekenisvolle impak op oorgewig en vetsugtige vrouens sou hê nie, asook hoe blywend enige veranderinge sou wees nie. Die hoofdoel van hierdie studie was dus om die inoefening- en die geen-inoefening effekte van ʼn korttermyn HIIO program op geselekteerde gesondheidskenmerke in jong oorgewig en vetsugtige dames te bepaal. ʼn Nie-ewekansige steekproef van 20 oorgewig en vetsugtige vrouens (18-25 jaar) het vrywillig ingestem om aan hierdie studie deel te neem. Geselekteerde gesondheidskenmerke is voor die aanvang van die inoefening gemeet. Die HIIO intervensie het twee weke geduur en het uit ses sessies bestaan (10 – 15x1 minuut draf by 90% HSmaks en een minuut aktiewe herstel by 50-60% HSmaks). Die HIIO intervensie is deur ʼn na-toets gevolg waarin basislyn metings herhaal is. Dit is deur ʼn twee weke geen-inoefening periode en opvolgtoetse opgevolg. Die hoofbevinding van hierdie studie was dat ses sessies van HIIO fisiologiese aanpassings na vore gebring het wat die risiko profiele van jong oorgewig en vetsugtige vrouens verlaag het. Daar was statisties betekenisvolle afnames in liggaamsmassa (1.6%, p < 0.001), vetmassa (3.7%, p < 0.001) en heupomtrek (4.8%, p < 0.001) en ʼn toename in vetvrye liggaamsmassa van 1.9% (p < 0.001). Daar was ook ʼn afname in bloedglukose (11%, p < 0.001), totale cholesterol (10.4 %, p = 0.01), sistoliese (3.4%, p < 0.001) en diastoliese bloeddruk (5.8%, p < 0.001). Daar was ook statisties betekenisvolle verbeteringe in relatiewe VO2maks en oefeningtoleransie na inoefening. Die opvolgtoetse na twee weke van geen-inoefening het getoon dat metaboliese aanpassings wat deur die HIIO bereik is, relatief blywend van aard was of ten minste nie totaal omgekeerd was nie. Die gewigsverlies wat deur die HIIO veroorsaak was is belangrik in die sin dat dit die hoofdoelwit aanspreek om die voorkoms van oorgewig en vetsugtigheid te verminder. Die studie suggereer verder dat 10 – 15 minute HIIO werksessies, by naby maksimale intensiteite, moontlik die minimum hoeveelheid inoefening is wat benodig word om betekenisvolle gewigsverlies en ander positiewe gesondheidskenmerke te bereik.

O treinamento resistido intervalado de alta intensidade (high intensity interval resistance training-HIIRT) tem se tornado cada vez mais popular nos últimos anos poispromoveresultados positivos com curtas sessões de treinamento. No entanto, sua alta intensidade pode causar efeitos adversos. O objetivo deste estudo foi avaliar se uma sessão de HIIRT causa dano muscular agudo e alterações nos biomarcadores de lesão renal. Cinquenta e oito voluntários saudáveis, divididos igualmente entre homens e mulheres com 24 anos de idade (mediana), participaram deste estudo. Nenhum deles usou suplementos dietéticos ou medicamentos. Eles foram submetidos a cinco minutos de aquecimento seguido de quatro minutos de HIIRT. Uma escala numérica de Borg para dor (CR10P), amostras de sangue e urina foram coletadas antes (basal), 2 e 24h após a sessão HIIRT. As amostras de sangue foram analisadas e dosadocreatinina sérica (CrS),lipocalina associada a gelatinase de neutrófilos (NGALS), creatinofosfoquinase (CPK) e mioglobina (Mio). As amostras urinárias foram analisadas e dosadoscreatinina (CrU),lipocalina associada a gelatinase de neutrófilos (NGALU), interleucina 18 (IL-18), calbindina, microalbuminúria (?albumina), trefoil factor-3(TFF3) e beta-2microglobulina (ß2M). O CR10 e CPK tiveramum aumento significativo e crescente após2 e 24h. Mioaumentou significativamente em 2h e continuouelevadaapós24h. CrSaumentou significativamente após24h e em três homens,o aumento atingiu os critérios para o diagnóstico de injúria renal aguda (IRA). Todos os biomarcadores urinários aumentaram significativamente 2 horas após o exercício e retornaram aos valores basais24h após HIIRT. Concluindo, uma única sessão de HIIRT em indivíduos jovens e saudáveis causou elevações precocese significativas em CPK, mioglobina, CrS,microalbuminúria e biomarcadores urinários, indicando lesão tubularrenal, sugerindo a ocorrência de rabdomiólise e danos funcionais eestruturais aos rins / High intensity interval resistance training (HIIRT) emerged as one of the fastest growing exercise programs in recent years because provides positive results with short training sessions. However, its high intensity might cause adverse effects. The aim of this study was to evaluate if a session of HIIRT causes acute muscle damage and changes in kidney injury biomarkers. Fifty-eight healthy volunteers, divided equally among men and women (median age 24 years), participated in this study. None of them used dietary supplements or medications. They were submitted to five minutes of warm-up followed by four minutes of HIIRT. A Borg CR10 Scale for pain (CR10P), and blood and urinary samples were collected before (baseline), 2 and 24h after HIIRT session. Blood samples were analyzed for serum creatinine (SCr), neutrophil gelatinase-associated lipocalin (SNGAL), creatine kinase(CK) and myoglobin (Myo). Urinary samples were assessed for creatinine (UCr), neutrophil gelatinase-associated lipocalin (UNGAL), interleukin 18 (IL-18), calbindin, microalbuminuria (ualbumin), trefoil factor-3 (TFF3) and beta-2 microglobulin (beta2M). CR10 had a significantly and crescent increase on 2 and 24h. CK increased significantly on 2h and further in 24h. Myo increased significantly on 2h and stayed elevated at 24h. SCr increased significantly on 24h and inthree men the increase met criteria for acute kidney injury diagnosis. All the other serum and urinary kidney injury biomarkers increased significantly at 2 hours and returned to basal values at 24h after HIIRT. In conclusion, a single session of HIIRT inyoung, healthy individuals caused early and significant elevations in CK, myoglobin, SCr, microalbuminuria and urinary biomarkers indicative of kidney tubular injury, suggesting the occurrence of rhabdomyolysis and functional and structural kidney damage

Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-08-08T14:11:40Z No. of bitstreams: 1 ArilsonFernandesMendoncadeSousaTese2018.pdf: 5768876 bytes, checksum: 66d7e7ce78b9d2a4b54e615ac9d3282d (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-08-08T14:11:52Z (GMT) No. of bitstreams: 1 ArilsonFernandesMendoncadeSousaTese2018.pdf: 5768876 bytes, checksum: 66d7e7ce78b9d2a4b54e615ac9d3282d (MD5) / Made available in DSpace on 2018-08-08T14:11:52Z (GMT). No. of bitstreams: 1 ArilsonFernandesMendoncadeSousaTese2018.pdf: 5768876 bytes, checksum: 66d7e7ce78b9d2a4b54e615ac9d3282d (MD5) Previous issue date: 2018-05-25 / Only two weeks of sprint interval training (SIT) has been shown to be associated with positive changes in aerobic capacity and cardiac autonomic control. Both aerobic capacity and autonomic control have been shown to be positively associated with improved attention. However, to date, the relationship between this type of training and attention has not been investigated yet. The aim of the present study was to investigate the influence of two weeks of SIT on aerobic capacity, cardiac autonomic control and attention components in healthy university students; Also, to verify if the training fidelity would influence these adaptations. One hundred and nine participants were divided into experimental (EG) and control (CG) groups. EG performed a SIT program consisting of 6 sessions of maximal 4 × 30 s all-out efforts on a cycle ergometer, interspersed with active 4-minute rests. The criterion for fidelity was to reach> 90% of the estimated maximum heart rate (HR) during sprint sessions. After analysis, EG was divided into fidelity groups HIGH (n = 26) and LOW (n = 46), respectively. The attention components were evaluated through the Attention Network Test (ANT). The aerobic capacity (VO2max) was estimated according to Astrand's nomogram while the sum of the skinfolds: pectoral, triceps, subscapular, medial axillary, abdomen, suprailiac and thigh was verified. Autonomic HR control was assessed by HR variability (HRV) and HR complexity at rest and during ANT, before and after six sessions of SIT. Both HIGH and LOW significantly increased aerobic capacity, vagal modulation before and during ANT and executive control, and decreased body fat after SIT (p <0.05). However, only HIGH participants showed an increase in HR complexity and accuracy in ANT responses when compared to LOW (p <0.05). Two weeks of SIT improved executive control, body fat, aerobic capacity and autonomic control in university students, with better results reported for the HIGH group. / Apenas duas semanas de treinamento intervalado de esforços supra máximos (TIsm) tem mostrado estar associado com modificações positivas na capacidade aeróbia e controle autonômico cardíaco. Tanto a capacidade aeróbia, como o controlo autonômico demonstram estar associados positivamente com melhoria da atenção. Entretanto, até o presente momento a relação entre este tipo de treinamento e atenção não foi investigada. O objetivo do presente estudo foi investigar a influência de um programa de TIsm na capacidade aeróbia, controle autonômico cardíaco e componentes da atenção em jovens universitários saudáveis; ainda, verificar se a fidelidade do treinamento influenciaria essas adaptações. Cento e nove participantes foram divididos em grupo experimental (GE) e controle (GC). O GE realizou um programa TIsm que consistiu em 6 sessões de TIsm de 4 × 30 s em um cicloergômetro, intercaladas com descansos ativos de 4 min. O critério para fidelidade foi atingir> 90% da frequência cardíaca máxima estimada (FC) durante as sessões de TIsm. Após as análises, o GE foi dividido em grupos de fidelidade alta, GEA (n = 26) e baixa, GEB (n = 46), respectivamente. Os componentes da atenção foram avaliados por meio do Teste de Rede de Atenção (ANT). A capacidade aeróbia (VO2max) foi estimada segundo o nomograma de Astrand enquanto o somatório de dobras cutâneas: peitoral, tríceps, subescapular, axilar média, abdômen, supra-ilíaca e coxa foi realizada. O controle autonômico da FC foi avaliado por meio da VFC e complexidade da FC em repouso e durante o ANT, antes e depois de seis sessões de TIsm. Ambos GEA e GEB aumentaram significativamente a capacidade aeróbia, modulação vagal antes e durante a realização do ANT e o controle executivo e diminuição da gordura corporal após o TIsm (p <0,05). No entanto, apenas os participantes do GEA apresentaram um aumento na complexidade da FC e acurácia nas respostas do ANT quando comparados ao GEB (p <0,05). Duas semanas de TIsm melhoraram o controle executivo, a gordura corporal, a capacidade aeróbia e o controle autonômico em estudantes universitários, com melhores resultados relatados para o grupo com GEA fidelidade.

L’exercice fractionné de haute intensité (HIIT) et continu d’intensité modéré (MOD) représentent les 2 grandes modalités d'endurance. Cependant, leur impact spécifique sur la performance physique et la plasticité cérébrale et musculaire reste controversé du fait de la diversité des protocoles d’exercice proposés chez les sédentaires. Notre 1er axe dégage chez des rats sains l’effet de ces 2 modalités sur la performance physique, la plasticité musculaire et cérébrale sur 8 semaines d’entrainement standardisée dont l'intensité est basées sur le seuil lactique. Nos résultats montrent des gains de performance d’endurance plus rapides et importants suite aux HIIT. La neuroplasticité serait stimulée par les HIIT uniquement et la plasticité musculaire semble spécifique à chacune. L’engouement pour les HIIT se répercute chez les patients subissant un accident vasculaire cérébral où l’efficacité des méthodes d’endurance n’est pas clairement justifiées. Une étude antérieure a montré l’intérêt majeur des HIIT dans la phase aiguë de la pathologie malgré une récupération sensorimotrice incomplète. D’où l’intérêt d’approfondir dans notre second axe l’effet des différentes formes de HIIT (longs et courts) dans la récupération. Nos résultats montrent principalement que ces deux formes de HIIT améliorent la capacité d’endurance et la force de la patte antérieure lésée avec une précocité pour les HIIT longs. Les deux formes de HIIT semblent induire une angiogenèse cérébrale. Néanmoins, ils n’améliorent pas les fonctions sensorimotrices et cognitives. Ainsi, il est nécessaire d’approfondir les répercussions de ces deux entrainements HIIT dans la plasticité musculaire et cérébrale. / Endurance exercise is essential for different reasons in athlete and also in aging and pathological people. Two training modalities were found: high intensity interval training (HIIT) and moderate intensity aerobic training (MOD). However, the specific outcomes of these modalities on physical performance and cerebral and muscular plasticity are controversial because many exercise protocols exist. The 1st study explore the impact of these 2 training on endurance and functional capacity and also on muscular and cerebral molecular modifications throughout 8 weeks in healthy rats. HIIT and MOD programs are work-matched and training intensity are determined thanks to the lactate threshold. Our results show a superior and fast effect on endurance capacity after HIIT compared to MOD. Hippocampal plasticity is stimulated only after HIIT and muscular modifications appear to be specific to each modality. A great interest of HIIT is found in stroke patients for whom evidence of endurance modalities efficiency is still missing. A previous study has shown a beneficial effect of HIIT in the acute phase of stroke despite incomplete sensorimotor recuperation. Hence, the interest to deepen in second part of this manuscript the impact of two HIIT modalities (short and long) in recovery optimisation. Our results show that 2 HIIT strongly improve endurance performance and strength of injured paw with a fast effect for long HIIT. The 2 modalities seem to induce cerebral angiogenesis. However, these 2 training do not increase sensorimotor and cognitive functions. In perspective, it appears necessary to develop muscular and cerebral outcomes induced by these 2 HIIT modalities.

This thesis examined the translocation of fatty acid (FA) transport proteins to the plasma membrane (PM) in human and rat skeletal muscle during moderate intensity exercise. The responses to the post-exercise period and to acute moderate intensity exercise after 6 weeks of high intensity interval training (HIIT) were also examined in humans. The overall hypotheses were that 1) FAT/CD36 and FABPpm would translocate to the PM in human skeletal muscle during 120 min of moderate intensity exercise, 2) FAT/CD36 and FABPpm would translocate to the PM in rat skeletal muscle during 120 min of moderate intensity exercise and this would correlate to an increase in palmitate uptake, 3) FAT/CD36 and FABPpm would translocate to the PM during 120 min of moderate intensity exercise, but return to basal levels by 45 min post-exercise, 4) six weeks of HIIT would increase PM content of FABPpm but not FAT/CD36 in resting skeletal muscle, 5) six weeks of HIIT would cause a further increase in the translocation of FAT/CD36 and FABPpm to the PM during moderate intensity exercise and this would correspond to an increase in whole body fat oxidation compared to exercise pre-training, and 6) six weeks of HIIT would increase whole muscle content of FATP1 and FATP4. In human skeletal muscle, PM FAT/CD36 and FABPpm increased 75% and 20% respectively after 120 min of cycling at ~60% VO2 peak which corresponded to a 110% increase in whole body fat oxidation. In rat skeletal muscle, PM FAT/CD36 and FABPpm increased 20% and 30% respectively, which correlated to a 30% increase in palmitate uptake following 120 min of treadmill running at ~65% VO2 peak. The PM content of FAT/CD36 increased further to 120% of resting values by 45 min of post-exercise following 120 min of cycling at ~60% VO2peak, which correlated with a heavy reliance on fat as a fuel during the post-exercise period. FABPpm returned to resting levels of PM content by 15 min post-exercise. After 6 wk of HIIT, whole muscle FAT/CD36 (50%), FABPpm (21%) and FATP4 (25%) were increased in human skeletal muscle, while FATP1 remained unchanged. There were no changes in PM content of FAT/CD36 or FABPpm at rest following training. FAT/CD36 and FABPpm were also measured before and after 120 min of cycling at ~60% of pre-trainingVO2 peak following training, but no differences in the magnitude of the PM content increases were seen compared to pre-training, despite a 27% increase in fat oxidation. These studies demonstrate that FA transport proteins translocate to the PM during moderate intensity exercise, which correlates with increased FA uptake and whole body fat oxidation. This relationship does not appear to hold during the post-exercise period, as further increases in the PM content of FAT/CD36 does not correspond with the decrease in fat oxidation. The PM content of FAT/CD36 and FABPpm were not increased at rest following training, and there was no effect of training on the translocation of FAT/CD36 or FABPpm to the PM during moderate intensity exercise at the same absolute power output, however there may be a further increase at a relative power output. / Natural Sciences and Engineering Research Council, Canadian Institute of Health Research, Ontario Graduate Scholarship

Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2018-05-03T14:05:56Z No. of bitstreams: 2 Dissertação - Marco Antonio Basso Filho - 2018.pdf: 1179214 bytes, checksum: c42f7002852698e32ce9c89e0baff62d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-05-03T15:41:54Z (GMT) No. of bitstreams: 2 Dissertação - Marco Antonio Basso Filho - 2018.pdf: 1179214 bytes, checksum: c42f7002852698e32ce9c89e0baff62d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-05-03T15:41:54Z (GMT). No. of bitstreams: 2 Dissertação - Marco Antonio Basso Filho - 2018.pdf: 1179214 bytes, checksum: c42f7002852698e32ce9c89e0baff62d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Introduction: Studies show that high intensity interval training (HIIT) promotes improvements equivalent or even superior to continuous moderate intensity training (IMT). However, HIIT has divergent applications, approaches and results in the literature regarding its physiological repercussions and the parameters that may bring more safety in its prescription. In this context kinetics and heart rate variability (HRV) represent the physiological responses of the autonomic nervous system (ANS) in cardiac autonomic heart rate (HR) modulation, as well as VO2max represents the aerobic functional capacity and, in the rest-exercise transitions and exercise-recovery, can demonstrate the efficiency of different physical training in fitness, adaptation, vagal withdrawal and sympathetic hyperactivity. Objective: To compare the acute effects of different intensities of physical training on the kinetics of HR and HRV, and functional capacity in healthy physically active youngsters. Objective: To compare the acute effects of different intensities of physical training on the kinetics of HR and HRV, and functional capacity in healthy physically active youngsters. Methodology: Clinical crossover performed with 12 physically active young males. The procedure to obtain the data occurred for 4 days with an interval of 48 hours between those days. On the 1st day the participants were submitted to evaluations including the cardiopulmonary exercise test (CPT) for the purpose of verifying VO2max, identifying their aerobic functional capacity, as well as prescribing the protocols. Between the 2nd and 4th day, the three exercise protocols were performed: TCMI (exercise = 21 minutes continuous at 70% of vVO2max), short HIIT (exercise = 29 repetitions of 30 seconds at 100% of vVO2max and recovery = 30 seconds passive to 50% of vVO2max) and long HIIT (exercise = 3 replicates of 4 minutes to 90% of vVO2max and recovery = 3 minutes to 60% of vVO2max). Participants performed 5 minutes of heating at 55% of vVO2max and at the end of the session 3 minutes of recovery at 50% of vVO2max. The recording data of the R-R intervals were recorded with the participant lying in the supine position for 10 minutes before the start of the tests and immediately after the recovery of the sit-in tests for 3 minutes. The behavior of the HR, HRV and VO2max variables were verified in the rest-exercise transition, during exercise and in the exercise-recovery transition. Statistical analysis: The normality of the data was evaluated by the Komolgorov-Smirnov test. In order to evaluate the correlation between HRV indexes after each exercise protocol and VO2peak, the Pearson Correlation Test or the Spearman Correlation Test were used. Two-way ANOVA was used to verify the differences between the variables obtained in each exercise protocol (HRV indices and cardiorespiratory fitness variables). Values of p <0.05 were considered significant. Results: The systolic blood pressure (SBP) variable showed a lower value in the short HIIT when compared to the long HIIT (p = 0.003). The analysis of FCon kinetics showed a statistical difference for the delta time variable (∆T) when we compared the IMT and the long HIIT (p = 0.041), observing that the IMT promoted a greater slowness in the HR response in the rest-exercise transition. The analysis of the HRV during the exercise-recovery phase (off) was different between the long HIIT versus the IMT and the long HIIT versus the short HIIT (p <0.005 and p = 0.012, respectively), and the long HIIT showed greater sympathetic modulation and lower parasympathetic modulation. The comparison of the differences between the deltas of FCoff, the long HIIT showed a lower HR reduction in the initial 60 seconds of recovery when compared to the short HIIT and IMT respectively, presented by delta 30 (p <0.001 and p = 0.034, respectively) and Delta 60 (p = 0.012 and p = 0.037, respectively). The VO2peak variable had a higher value in the long HIIT when compared to the IMT and the short HIIT (p = 0.028 and p <0.001, respectively). Conclusion: In the intergroup comparison the IMT presented better HR values in the rest-exercise transition and the long HIIT obtained better results in the variables VO2peak and FCpeak. In terms of HRV in the exercise-recovery transition the short HIIT demonstrated better adaptation in the autonomic modulation while the long HIIT showed greater sympathetic hyperactivity and consequent cardiac overload. / Introdução: Estudos demonstram que o treino intervalado de alta intensidade (HIIT) promove melhorias equivalentes ou até mesmo superiores ao treinamento contínuo de moderada intensidade (TCMI). No entanto, o HIIT tem aplicações, abordagens e resultados divergentes na literatura em relação as suas repercussões fisiológicas e dos parâmetros que possam trazer mais segurança em sua prescrição. Neste contexto, a cinética e a variabilidade da frequência cardíaca (VFC) representam as respostas fisiológicas do sistema nervoso autônomo (SNA) na modulação autonômica da frequência cardíaca (FC), assim como o VO2máx representa a capacidade funcional aeróbia e, nas transições repouso-exercício e exercício- recuperação, podem demonstrar a eficiência de diferentes treinos físicos na aptidão, adaptação, retirada vagal e hiperatividade simpática. Objetivo: Comparar os efeitos agudos de diferentes intensidades de treinamento físico sobre a cinética da FC e VFC, e capacidade funcional em jovens saudáveis fisicamente ativos. Metodologia: Ensaio clínico crossover realizado com 12 jovens do sexo masculino ativos fisicamente. O procedimento para obtenção dos dados ocorreu por 4 dias com intervalo de 48 horas entre esses dias. No 1º dia os participantes foram submetidos a avaliações, entre elas o teste de esforço cardiopulmonar (TECP), com a finalidade de verificar VO2máx, identificar sua capacidade funcional aeróbia, bem como para prescrever os protocolos. Entre o 2º e o 4º dia foram realizados os três protocolos de exercícios: TCMI (exercício = 21 minutos contínuos a 70% da vVO2máx), HIIT curto (exercício = 29 repetições de 30 segundos a 100% da vVO2máxe recuperação = 30 segundos passivos a 50% da vVO2máx) e HIIT longo (exercício = 3 repetições de 4 minutos a 90% da vVO2máx e recuperação = 3 minutos a 60% da vVO2máx). Os participantes realizaram 5 minutos de aquecimento a 55% da vVO2máx e ao término da sessão 3 minutos de recuperação a 50% da vVO2máx.Os dados da gravação dos intervalos R-R foram registrados com o participante deitado em posição supina por 10 minutos antes do início dos testes e imediatamente após o término da recuperação dos testes em postura sentada por 3 minutos. O comportamento das variáveis FC, VFC e VO2máx foram verificados na transição repousoexercício, durante o exercício e na transição exercício-recuperação. Análise estatística: A normalidade dos dados foi avaliada pelo Teste de Komolgorov-Smirnov. Para avaliar a correlação entre os índices da VFC após cada protocolo de exercício e o VO2pico foram utilizados o Teste de Correlação de Pearson ou o Teste de Correlação de Spearman. A ANOVA twoway foi utilizada para verificar as diferenças entre as variáveis obtidas em cada protocolo de exercício (índices da VFC e variáveis da aptidão cardiorrespiratória). Foram considerados significativos valores de p < 0,05. Resultados: A variável pressão arterial sistólica (PAS) mostrou menor valor no HIIT curto quando comparada ao HIIT longo (p = 0.003). A análise da cinética da FCon demonstrou diferença estatística para a variável delta tempo (∆T) ao compararmos o TCMI e HIIT longo (p = 0.041), observando que o TCMI promoveu maior lentificação na resposta da FC na transição repouso-exercício. A análise da VFC durante a fase de exercício-recuperação (off) mostrou-se diferente entre o HIIT longo versus TCMI e HIIT longo versus HIIT curto (p < 0.005 e p = 0.012, respectivamente), sendo que o HIIT longo mostrou maior modulação simpática e menor modulação parassimpática. A comparação das diferenças entre os deltas da FCoff, o HIIT longo apresentou menor redução da FC nos 60 segundos iniciais da recuperação quando comparados ao HIIT curto e TCMI respectivamente, apresentado pelo delta 30 (p < 0.001 e p = 0.034, respectivamente) e Delta 60 (p = 0.012 e p = 0.037, respectivamente). A variável VO2pico apresentou maior valor no HIIT longo quando comparado ao TCMI e ao HIIT curto (p = 0.028 e p < 0.001, respectivamente). Conclusão: Na comparação intergrupos o TCMI apresentou melhores valores de FC na transição repousoexercício e o HIIT longo obteve melhores resultados nas variáveis VO2pico e FCpico. Em termos de VFC na transição exercício-recuperação, o HIIT curto demonstrou melhor adaptação na modulação autonômica enquanto o HIIT longo mostrou maior hiperatividade simpática e consequente sobrecarga cardíaca.

High intensity interval training (HIIT) has become very popular due to the dissemination of experimental results. HIIT promotes similar adaptations to longterm and low-intensity exercises, such as improvement in cardiorespiratory capacity, VO2max, respi- ratory metabolism, increased pulmonary ventilation, among others. However, these adaptations are obtained with much lower duration at maximum or submaximal intensities, with seconds or few minutes of interval. However, frequent performance may increase susceptibility to injury, promote chronic fatigue and overtraining, partly because of the high synthesis of reactive oxygen species (ROS). Stress is a state of imbalance between oxidation and oxidation reactions. However, the effects of HIIT on oxidative status and muscle damage are still not well understood in the scientific literature. The objective of this dissertation was to verify the effects of short-term HIIT on biomarkers of oxidative stress and muscle damage in Wistar rats. Thus, three studies were elaborated: 1) The effects of short-term HIIT on the markers of oxidative stress and muscle damage were evaluated; 2) It was sought to characterize the responses of cardiac oxidative stress markers to HIIT in rats. 3) The effects of twelve consecutive sessions and on different days of HIIT on the markers of hepatic oxidative stress were verified. In studies 1 and 2 significant changes were found in only one marker of oxidative damage, a fact that did not happen in study 3. Still on study 1 there were no changes in the markers of muscle damage, however there was a significant reduction in a marker of defense antioxidant. In studies 2 and 3 there were no significant changes in antioxidant capacity. It is concluded that HIIT is performed on consecutive or distinct days, and depending on tissue it may or may not promote liver, heart and muscle damage in rats. / O treinamento intervalado de alta intensidade (HIIT) na comunidade científica tem se tornado muito notório em função da divulgação de estudos experimentais. O HIIT promove adaptações semelhantes aos exercícios de longa duração e baixa intensidade, tais como melhora da capacidade cardiorrespiratória, do metabolismo de respouso, aumento da ventilação pulmonar, entre outros. Porém, estas adaptações são obtidas com duração muito inferior em intensidades máximas ou submáximas, com segundos ou poucos minutos de intervalo. Contudo, a frequente realização pode aumentar a suscetibilidade a lesões, promover a fadiga crônica e overtraining, parcialmente em razão da elevada síntese de espécies reativas de oxigênio (EROs). Estresse oxidativo é um estado de desequilíbrio entre as reações de oxidação e de antioxidação. Entretanto, ainda existem lacunas a serem preenchidas acerca dos efeitos do HIIT sobre o estado oxidativo e danos musculares, sobretudo em modelo animal. O objetivo desta dissertação foi verificar os efeitos do HIIT de curto prazo sobre os biomarcadores de estresse oxidativo e danos musculares em ratos. Desta forma, foram elaborados três estudos: 1. Avaliou-se os efeitos do HIIT de curto prazo sobre os marcadores de estresse oxidativo e danos musculares; 2. Buscou-se caracterizar as respostas dos marcadores de estresse oxidativo cardíaco ao HIIT em ratos. 3. Verificou-se os efeitos de doze sessões consecutivas e em dias distintos de HIIT sobre os marcadores de estresse oxidativo hepático. Nos estudos 1 e 2 foram encontradas alterações significativas em apenas um marcador de dano oxidativo, fato este que não aconteceu no estudo 3. Ainda sobre o estudo 1 não houveram alterações nos marcadores de danos musculares, no entanto houve uma redução significativa num marcador de defesa antioxidante. Nos estudos 2 e 3 não houveram mudanças significativas na capacidade antioxidante. Conclui-se que o HIIT, seja realizado em dias consecutivos ou distinitos, e a depender do tecido pode ou não promover danos hepáticos, cardíacos e musculares em ratos. / São Cristóvão, SE

O treinamento resistido intervalado de alta intensidade (high intensity interval resistance training-HIIRT) tem se tornado cada vez mais popular nos últimos anos poispromoveresultados positivos com curtas sessões de treinamento. No entanto, sua alta intensidade pode causar efeitos adversos. O objetivo deste estudo foi avaliar se uma sessão de HIIRT causa dano muscular agudo e alterações nos biomarcadores de lesão renal. Cinquenta e oito voluntários saudáveis, divididos igualmente entre homens e mulheres com 24 anos de idade (mediana), participaram deste estudo. Nenhum deles usou suplementos dietéticos ou medicamentos. Eles foram submetidos a cinco minutos de aquecimento seguido de quatro minutos de HIIRT. Uma escala numérica de Borg para dor (CR10P), amostras de sangue e urina foram coletadas antes (basal), 2 e 24h após a sessão HIIRT. As amostras de sangue foram analisadas e dosadocreatinina sérica (CrS),lipocalina associada a gelatinase de neutrófilos (NGALS), creatinofosfoquinase (CPK) e mioglobina (Mio). As amostras urinárias foram analisadas e dosadoscreatinina (CrU),lipocalina associada a gelatinase de neutrófilos (NGALU), interleucina 18 (IL-18), calbindina, microalbuminúria (?albumina), trefoil factor-3(TFF3) e beta-2microglobulina (ß2M). O CR10 e CPK tiveramum aumento significativo e crescente após2 e 24h. Mioaumentou significativamente em 2h e continuouelevadaapós24h. CrSaumentou significativamente após24h e em três homens,o aumento atingiu os critérios para o diagnóstico de injúria renal aguda (IRA). Todos os biomarcadores urinários aumentaram significativamente 2 horas após o exercício e retornaram aos valores basais24h após HIIRT. Concluindo, uma única sessão de HIIRT em indivíduos jovens e saudáveis causou elevações precocese significativas em CPK, mioglobina, CrS,microalbuminúria e biomarcadores urinários, indicando lesão tubularrenal, sugerindo a ocorrência de rabdomiólise e danos funcionais eestruturais aos rins / High intensity interval resistance training (HIIRT) emerged as one of the fastest growing exercise programs in recent years because provides positive results with short training sessions. However, its high intensity might cause adverse effects. The aim of this study was to evaluate if a session of HIIRT causes acute muscle damage and changes in kidney injury biomarkers. Fifty-eight healthy volunteers, divided equally among men and women (median age 24 years), participated in this study. None of them used dietary supplements or medications. They were submitted to five minutes of warm-up followed by four minutes of HIIRT. A Borg CR10 Scale for pain (CR10P), and blood and urinary samples were collected before (baseline), 2 and 24h after HIIRT session. Blood samples were analyzed for serum creatinine (SCr), neutrophil gelatinase-associated lipocalin (SNGAL), creatine kinase(CK) and myoglobin (Myo). Urinary samples were assessed for creatinine (UCr), neutrophil gelatinase-associated lipocalin (UNGAL), interleukin 18 (IL-18), calbindin, microalbuminuria (ualbumin), trefoil factor-3 (TFF3) and beta-2 microglobulin (beta2M). CR10 had a significantly and crescent increase on 2 and 24h. CK increased significantly on 2h and further in 24h. Myo increased significantly on 2h and stayed elevated at 24h. SCr increased significantly on 24h and inthree men the increase met criteria for acute kidney injury diagnosis. All the other serum and urinary kidney injury biomarkers increased significantly at 2 hours and returned to basal values at 24h after HIIRT. In conclusion, a single session of HIIRT inyoung, healthy individuals caused early and significant elevations in CK, myoglobin, SCr, microalbuminuria and urinary biomarkers indicative of kidney tubular injury, suggesting the occurrence of rhabdomyolysis and functional and structural kidney damage

Submitted by Márcio Ropke (ropke13marcio@gmail.com) on 2017-02-10T12:16:47Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) anelita.dissertacao.final.2013.12.04a.pdf: 2072554 bytes, checksum: e3cf58b92127a94864f0503af16ab9ff (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-02-16T20:09:34Z (GMT) No. of bitstreams: 2 anelita.dissertacao.final.2013.12.04a.pdf: 2072554 bytes, checksum: e3cf58b92127a94864f0503af16ab9ff (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-02-16T20:14:02Z (GMT) No. of bitstreams: 2 anelita.dissertacao.final.2013.12.04a.pdf: 2072554 bytes, checksum: e3cf58b92127a94864f0503af16ab9ff (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-02-16T20:15:06Z (GMT). No. of bitstreams: 2 anelita.dissertacao.final.2013.12.04a.pdf: 2072554 bytes, checksum: e3cf58b92127a94864f0503af16ab9ff (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2013-08-30 / Sem bolsa / Introdução: Na prescrição do treinamento intervalado de alta intensidade (HIIT) o tempo de recuperação entre esforços é aspecto relevante. No entanto, o tipo de recuperação tem se mostrado como determinante do desempenho subsequente, e a maioria dos estudos tem indicado que a recuperação ativa (RA) tende a ser superior à passiva (RP). Especificamente quanto à RA, ela pode ser com o mesmo grupo muscular exercitado, com segmento corporal oposto ou com região do tronco. No entanto, esta última estratégia tem sido pouco investigada. Objetivo: Investigar os efeitos de dois tipos de RA nas respostas fisiológicas e no desempenho físico subsequente a treino intervalado de alta intensidade, considerando dois diferentes níveis de aptidão aeróbia. Materiais e métodos: O estudo foi desenvolvido com 25 homens, entre 18 e 35 anos, os quais foram alocados em um destes dois grupos: Alta Aptidão (AACR),Baixa Aptidão (BACR). Os envolvidos realizaram três dias de atividades. Na primeira visita foram mensurados: massa corporal, estatura, dobras cutâneas, frequência cardíaca, lactato sanguíneo e pressão sanguínea em repouso, além da realização de teste de potência máxima (Pmax) em cicloergômetro e teste de tempo limite (TLim a 120% da Pmax), quinze minutos após. A classificação em AACR ou BACR decorreu da Pmax obtida no teste progressivo em cicloergômetro, e alocação de acordo com a mediana. Nas vistas dois e três os sujeitos realizaram: i) aquecimento padronizado, ii) HIIT com dois blocos com quatro estímulos supramáximos cada (30 s a 60 rpm e com carga de 120% da Pmax, 30 s de recuperação passiva) e iii) TLim após o HIIT. Após aquecimento, entre os blocos do HIIT e antes do TLim, os envolvidos executaram um dos dois modos de recuperação ativa: recuperação ativa na bicicleta (bike) e recuperação ativa com exercícios estabilizadores do tronco (core). Na recuperação bike, pedalava-se a 30% da Pmax durante 3 min e na core, realizavamse três exercícios, com duração de 50 s cada um. Para análise dos dados, contou-se com estatística descritiva e os dados foram analisados com análise de variância de dois caminhos (nível de aptidão aeróbia e tipo de recuperação) com medidas repetidas. Assumiu-se p<0,05 como nível de significância. Resultados: Não houve diferença estatisticamente significante considerando nível de aptidão e tipo de recuperação para o TLim. Para recuperação core, BACR e AACR atingiram, respectivamente, 118±25 s e 142±62 s. Na recuperação bike, os valores foram de 110±24 s e 134±72 s. A recuperação bike proporcionou valores inferiores de lactato sanguíneo, mas apenas antes da segunda série de esforços (BACR: 3,62±0,76mmol para core e 2,99±0,90mmol para bike; AACR: 3,23±0,52mmol para core e 2,83±0,66mmol para bike, F=6,38, p=0,01). A9 recuperação core, por sua vez, diminui a frequência cardíaca de modo mais pronunciado antes da primeira e da segunda série do HIIT, bem como antes do TLim(F≥33,8, p<0,001). Conclusão: Considerando-se dois tipos de recuperação ativa e dois níveis de aptidão física aeróbia, não se observaram diferenças significantes no tempo limite após exercício intervalado de alta intensidade. A recuperação com uso de bicicleta apresentou maior remoção de lactato após a segunda série de esforço e a recuperação com exercícios do core exibiu maior contribuição na diminuição da frequência cardíaca durante os períodos de recuperação entre os dois blocos de esforços. / Introduction: In the prescription of high intensity interval training (HIIT) the recovery time between efforts is relevant aspect. However, the type of recovery has been shown to be a determinant of subsequent performance, and most studies have indicated that active recovery (RA) tends to be superior to passive (PR) recovery. Specifically regarding RA, it can be with the same muscle group exercised, with opposite body segment or with trunk region. At the However, the latter strategy has been little investigated. Objective: To investigate the effects of two types of RA on physiological responses and physical performance subsequent to high intensity interval training, considering two different levels of aerobic fitness. Materials and methods: The study was developed with 25 men, aged between 18 and 35 years, who were allocated in one of two groups: High Aptitude (AACR), Low Aptitude (BACR). Those involved had three days of activities. At the first visit, body mass, height, skinfolds, heart rate, blood lactate and resting blood pressure were measured, Of maximum power test (Pmax) on cycle ergometer and time-out test (TLim to 120% of Pmax), fifteen minutes after. The classification in AACR or BACR was from the Pmax obtained in the progressive test in cycle ergometer, and allocation according to the median. In the two and three views the subjects performed: i) standardized heating, ii) HIIT with two blocks with four supramaximal stimuli each (30 s at 60 rpm and with 120% load of Pmax, 30 s of passive recovery) and iii) TLim after Or HIIT. After heating, between the HIIT blocks and before the TLim, participants performed one of two modes of active recovery: active bike recovery and active recovery with core stabilization exercises. In the bike recovery, 30% of the Pmax was cycled for 3 min and in the core, three exercises were performed, each lasting 50 s. To analyze the data, we counted With descriptive statistics and data were analyzed with two-way analysis of variance (aerobic fitness level and recovery type) with repeated measurements. We assumed p <0.05 as a level of significance. Results: There was no statistically significant difference considering the level of fitness and type of recovery for TLim. For core recovery, BACR and AACR reached, respectively, 118 ± 25 s and 142 ± 62 s. In bike recovery, the values ​​were 110 ± 24 s and 134 ± 72 s. The bike recovery provided lower values ​​of blood lactate, but only before the second series of efforts (BACR: 3.62 ± 0.76mmol for core and 2.99 ± 0.90mmol for bike; AACR: 3.23 ± 0.52mmol for core and 2.83 ± 0.66mmol for bike, F = 6.38 , P = 0.01). A9 core recovery, in turn, decreases heart rate more pronouncedly before the first and second series of HIIT, as well as before TLim (F≥33.8, p <0.001). Conclusion: Considering two types of active recovery and two levels of aerobic physical fitness, no significant differences were observed in the time limit after high intensity interval exercise. Recovery with bicycle showed greater lactate removal after the second effort series and recovery with core exercises showed a greater contribution in decreasing heart rate during the recovery periods between the two blocks of effort.