Software development is a process in continuous evolution. This characteristic implies also continuous changes in the functionality of the system under development. Some of these changes may cause problems when they are not properly and timely propagated to the project members. The aim of our research is to obtain a good understanding of problems caused by the lack of awareness of changes to features during a distributed software development project, to identify information and artifact repositories used by contributors, and then to draw the requirements of an awareness mechanism to tackle the awareness problem. In order to accomplish our research goals. we conducted a four month long case study at IBM Ottawa Software Lab. which we observed the collaboration patterns of a multi-site development project team. Our findings helped us identify the most important communication media that support development. In particular, we observed that the 3-1% of communication was by phone and via face-to-face interactions. and email was mostly used to alert contributors about changes to features. We also found that changes were not properly and timely propagated due to different corporate cultures of the project teams. Finally, we found that a high volume of communication makes developers prone to overlook important information that can lead to the generation of errors during development., These findings led us to believe that miscommunication and non-timely communication of changes related to feature development caused the release of code that created failures in stable builds. To address this problem. we developed the concept of a relationship to link developers to features. Using this concept, we have designed a feature-based Awareness Mechanism System to collect information, create relationships and deliver awareness information to the contributors involved in the implementation of a feature.

Bacteria are the oldest, structurally simplest, and most abundant forms of life on earth. Its detection has always been a serious question since the emerging of modern science and technology. There has been a phenomenal growth in the field of real-time bacteria detection in recent years with emerging applications in a wide range of disciplines, including medical analysis, food, environment and many more. Two important analytical functions involved in bacteria detection are cell lysis and polymerase chain reaction (PCR). Cell lysis is required to break cells open to release DNA for use in PCR. PCR is required to reproduce millions of copies of the target genes to reach detection limit from a low DNA concentration. Conventionally, cell lysis and PCR are performed separately using specialized equipments. Those bulky machines consume much more than needed chemical reagents and are very time consuming. An efficient, cost-effective and portable solution involving Nanotechnology and Lab-on-a-Chip (LOC) technology was proposed. The idea was to utilize the excellent antibacterial property of surface-functionalized nanoparticles to perform cell lysis and then to perform PCR on the same LOC system without having to remove them from the solution for rapid detection of bacteria. Nanoparticles possess outstanding properties that are not seen in their bulk form due to their extremely small size. They were introduced to provide two novel methods for LOC cell lysis to overcome problems of current LOC cell lysis methods such as low efficiency, high cost and complicated fabrication process. The first method involved using poly(quaternary ammonium) functionalized gold and titanium dioxide nanoparticles which were demonstrated to be able to lyse E. coli completely in 10 minutes. The idea originated from the excellent antibacterial property of quaternary ammonium salts that people have been using for a long time. The second method involved using titanium dioxide nanoparticles and a miniaturized UV LED array. Titanium dioxide bears photocatalytic effect which generates highly reactive radicals to compromise cell membranes upon absorbing UV light in an aqueous environment. A considerable reduction of live E. coli was observed in 60 minutes. The thesis then evaluates the effect of nanoparticles on PCR to understand the roles nanoparticles play in PCR. It was found that gold and titanium dioxide nanoparticles induce PCR inhibition. How size of gold nanoparticles affected PCR was studied as well. Effective methods were discovered to suppress PCR inhibition caused by gold and titanium dioxide nanoparticles. The pioneering work paves a way for the integration of nanoparticle cell lysis and LOC PCR for rapid detection of bacteria. In the end, an integrated system involving nanoparticle cell lysis and microchip PCR was demonstrated. The prototyped system consisted of a physical microchip for both cell lysis and PCR, a temperature control system and necessary interface connections between the physical device and the temperature control system. The research explored solutions to improve PCR specificity in a microchip environment with gold nanoparticles in PCR. The system was capable of providing the same performance while reducing PCR cycling time by up to 50%. It was inexpensive and easy to be constructed without any complicated clean room fabrication processes. It can find enormous applications in water, food, environment and many more.

A major focus of research in nanotechnology is the development of novel, high throughput techniques for fabrication of arbitrarily shaped surface nanostructures of sub 100 nm to atomic scale. A related pursuit is the development of simple and efficient means for parallel manipulation and redistribution of adsorbed atoms, molecules and nanoparticles on surfaces – adparticle manipulation. These techniques will be used for the manufacture of nanoscale surface supported functional devices in nanotechnologies such as quantum computing, molecular electronics and lab-on-achip, as well as for modifying surfaces to obtain novel optical, electronic, chemical, or mechanical properties. A favourable approach to formation of surface nanostructures is self-assembly. In self-assembly, nanostructures are grown by aggregation of individual adparticles that diffuse by thermally activated processes on the surface. The passive nature of this process means it is generally not suited to formation of arbitrarily shaped structures. The self-assembly of nanostructures at arbitrary positions has been demonstrated, though these have typically required a pre-patterning treatment of the surface using sophisticated techniques such as electron beam lithography. On the other hand, a parallel adparticle manipulation technique would be suited for directing the selfassembly process to occur at arbitrary positions, without the need for pre-patterning the surface. There is at present a lack of techniques for parallel manipulation and redistribution of adparticles to arbitrary positions on the surface. This is an issue that needs to be addressed since these techniques can play an important role in nanotechnology. In this thesis, we propose such a technique – thermal tweezers. In thermal tweezers, adparticles are redistributed by localised heating of the surface. This locally enhances surface diffusion of adparticles so that they rapidly diffuse away from the heated regions. Using this technique, the redistribution of adparticles to form a desired pattern is achieved by heating the surface at specific regions. In this project, we have focussed on the holographic implementation of this approach, where the surface is heated by holographic patterns of interfering pulsed laser beams. This implementation is suitable for the formation of arbitrarily shaped structures; the only condition is that the shape can be produced by holographic means. In the simplest case, the laser pulses are linearly polarised and intersect to form an interference pattern that is a modulation of intensity along a single direction. Strong optical absorption at the intensity maxima of the interference pattern results in approximately a sinusoidal variation of the surface temperature along one direction. The main aim of this research project is to investigate the feasibility of the holographic implementation of thermal tweezers as an adparticle manipulation technique. Firstly, we investigate theoretically the surface diffusion of adparticles in the presence of sinusoidal modulation of the surface temperature. Very strong redistribution of adparticles is predicted when there is strong interaction between the adparticle and the surface, and the amplitude of the temperature modulation is ~100 K. We have proposed a thin metallic film deposited on a glass substrate heated by interfering laser beams (optical wavelengths) as a means of generating very large amplitude of surface temperature modulation. Indeed, we predict theoretically by numerical solution of the thermal conduction equation that amplitude of the temperature modulation on the metallic film can be much greater than 100 K when heated by nanosecond pulses with an energy ~1 mJ. The formation of surface nanostructures of less than 100 nm in width is predicted at optical wavelengths in this implementation of thermal tweezers. Furthermore, we propose a simple extension to this technique where spatial phase shift of the temperature modulation effectively doubles or triples the resolution. At the same time, increased resolution is predicted by reducing the wavelength of the laser pulses. In addition, we present two distinctly different, computationally efficient numerical approaches for theoretical investigation of surface diffusion of interacting adparticles – the Monte Carlo Interaction Method (MCIM) and the random potential well method (RPWM). Using each of these approaches we have investigated thermal tweezers for redistribution of both strongly and weakly interacting adparticles. We have predicted that strong interactions between adparticles can increase the effectiveness of thermal tweezers, by demonstrating practically complete adparticle redistribution into the low temperature regions of the surface. This is promising from the point of view of thermal tweezers applied to directed self-assembly of nanostructures. Finally, we present a new and more efficient numerical approach to theoretical investigation of thermal tweezers of non-interacting adparticles. In this approach, the local diffusion coefficient is determined from solution of the Fokker-Planck equation. The diffusion equation is then solved numerically using the finite volume method (FVM) to directly obtain the probability density of adparticle position. We compare predictions of this approach to those of the Ermak algorithm solution of the Langevin equation, and relatively good agreement is shown at intermediate and high friction. In the low friction regime, we predict and investigate the phenomenon of ‘optimal’ friction and describe its occurrence due to very long jumps of adparticles as they diffuse from the hot regions of the surface. Future research directions, both theoretical and experimental are also discussed.

The specific nature of work in nuclear medicine departments involves the use of isotopes and handling procedures, which contribute to the considerable value of the equivalent dose received, in particular, by the fingertips. Workers of nuclear medicine units who label radiopharmaceuticals are exposed to ionizing radiation. The doses of nuclear medicine workers determined by individual dosimeters, which supply data on the magnitude of personal dose equivalent. The dosimetry pointing to a considerable optimization of the radiological protection among that professional group. However, the problem of the excessive hand exposure had been noted already in the early 1980s. Systematic studies were undertaken in West Scotland. The difficulties associated with automation of radiopharmaceutical preparation process are responsible for the continuing growth of exposure to the hands of the workers. Similar studies have also been undertaken, e.g. in Chile, Norway, Australia, Italy, USA, Belgium(1). In each case, special attention has been paid to the exposure of nuclear medicine worker hands. The radiopharmacists who label various ligands can be exposed to high radiation doses to their fingertips (primarily of the thumb, index finger and middle finger). Quite frequently, the Hp(0.07) to the fingertips of those three fingers may exceed the dose limit, i.e. value of 500 mSv/y for the skin of human fingers, this dose limit refers to the maximum dose recorded(2). Specific difficulty in assessing the exposures of the most affected finger parts is aggravated by the fact that the universally employed method for the determination of the radiation doses received by the hands using a ring with attached thermoluminescence detectors is not adequate in this particular case. This measurement method is inadequate because distribution of the doses received by the skin of the hands and fingers is extremely non uniform. The main aim of the study was to measure the absorbed dose at the hands of the personnel by using thermoluminescent detectors / -

Semiochemicals are chemicals used to communicate. Animals tend to use these e.g. to locate food sources or to find a suitable mate. In this study, the sex pheromone of the Indian meal moth, Plodia interpunctella, was analysed. Since this is an economically important species, it is mass-reared in labs and science centers worldwide for experimental purposes. A culture of these moths was brought into the lab at Lund University for studies and has after that served as a model species demonstrating up-wind pheromone-mediated male flight in different courses held by the university. As years went by, the culture got less successful in up-wind flights, most probably because of inbreeding and bottleneck effects, and therefore, a new culture was taken in. This study focuses on using various experiments to see if there was a behavioral and/or physiological difference between the two cultures. Results show a significant difference in behavioral traits (frequency of calling behavior in females and in male up-wind flights) but not in physiological traits (female pheromone production or male antennal response). This study discusses some effects of mass-reared lab cultures.

Orientador: Prof. Dr. Edson Pinheiro Pimentel / Dissertação (mestrado) - Universidade Federal do ABC, Programa de Pós-Graduação em Engenharia da Informação, 2015. / Several researchs have shown the difculties in Chemistry teaching and learning process. The exercise, as well as performing experiments involving Chemistry is not always are present in the life of students. The lack of laboratories and trained professionals to operate them, results in the dificulty of contextualize concepts because of the scarcity of experimental practices necessary for teaching and learning chemistry. Despite the educators' eforts to propose theoretical models and use diferent methodologies in teaching, research shows that due to lack of motivation, physical structure and insucient learning materials, it is increasingly necessary to use new technologies to assist in learning, making this process attractive, dynamic and modern in the learning environments. This work aims to create conditions to increase the availability of resources in order to increase the experimental practice at the Chemistry Teaching. In this sense, a Gamied Virtual Laboratory for the teaching of Chemical Transformations was developed using game elements. In addition, experiments were conducted with students to obtain the degree of satisfaction of users using the tool. The results indicate that the Virtual Laboratory contributes to student learning due to the similarities in experiments, and also the motivation provided by gamication, allowing educators use this tool as a support for the teaching and learning process of practical activities carried out in the laboratory.

Made available in DSpace on 2014-06-11T19:31:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-10-02Bitstream added on 2014-06-13T19:20:17Z : No. of bitstreams: 1 silva_dg_dr_jabo.pdf: 1249023 bytes, checksum: a507ea8e0f1eb8792694e386158a0c82 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O presente estudo teve como objetivo avaliar as alterações clínicas e laboratoriais de bezerros infectados experimentalmente com Salmonella Dublin e verificar o efeito do tratamento com antibiótico florfenicol associado ou não à fluidoterapia. Para isso, foram constituídos quatro grupos experimentais, compostos por seis bezerros cada, que receberam, por via oral, aproximadamente 108 UFC de S. Dublin (exceto o grupo 1) e que foram submetidos aos seguintes procedimentos: controle (grupo 1), sem tratamento (grupo 2), tratamento apenas com florfenicol (grupo 3) e tratamento com florfenicol associado à fluidoterapia (grupo 4). Todos os bezerros foram submetidos ao exame físico antes da inoculação experimental e a cada 12 horas, até o sétimo dia após a infecção. Nestes momentos foram colhidas amostras de sangue, para realização de exames hematológicos, hemogasométricos, bioquímicos e proteinograma, e suabes retais, para isolamento de Salmonella por meio de exames microbiológicos e da reação em cadeia da polimerase (PCR). A infecção experimental com S. Dublin induziu diarréia, febre, desidratação e sinais respiratórios nos bezerros inoculados e provocou aumento da contagem do número de leucócitos, da concentração de uréia, fibrinogênio e proteínas de fase aguda, e redução do valor do pH, do excesso de base, da concentração de albumina, magnésio e ferro. Os bezerros que receberam tratamento mostraram boa recuperação clínica, sendo que o grupo tratado com antibiótico associado à fluidoterapia apresentou correção mais rápida e eficiente do equilíbrio hidro-eletrolítico. Quanto à detecção de Salmonella Dublin, o isolamento microbiológico mostrou-se superior à PCR, contudo o uso simultâneo das duas técnicas propiciou o diagnóstico de um maior número de amostras positivas. / The aim of the study was to evaluate clinical and laboratorial changes in experimentally Salmonella Dublin-infected calves and the effect of florfenicol antibiotic treatment associated or not with fluid therapy. Four experimental groups, comprising six calves, were formed. With the exception of group 1, animals orally received about 108 CFU of S. Dublin and underwent the following procedures: control (group 1), without any treatment (group 2), treatment with florfenicol only (group 3), treatment with florfenicol associated with fluid therapy (group 4). All calves were submitted to physical examination before experimental inoculation and at every 12 hours up to the 7th day after infection. Blood samples were collected for hematological, hemogasometric, and biochemical analysis, coupled to rectal swabs for the isolation of Salmonella by standard microbiological techniques and polymerase chain reaction (PCR). S. Dublin experimental infection caused diarrhea, fever, dehydration and respiratory effects in inoculated calves; increased values of leukocytes, urea, fibrinogen and acute phase proteins; decreased rate of pH, base excess, albumin, magnesium and iron. Treated calves had a satisfactory clinical recovery. The group treated with antibiotics associated with fluid therapy had a faster and more efficient correction of hydro-electrolyte alterations. Although microbiological isolation was better than PCR in the detection of Salmonella, the simultaneous use of both techniques provides a diagnosis with more positive samples.

Submitted by MARIANA DE AVELAR GALVINO LIMA null (marianagalvino@hotmail.com) on 2018-02-20T12:35:40Z No. of bitstreams: 1 dissertação.pdf: 3429530 bytes, checksum: 08ea04bbed62f3ef05e2b15f151144d0 (MD5) / Approved for entry into archive by Adriana Aparecida Puerta null (dripuerta@rc.unesp.br) on 2018-02-20T13:56:23Z (GMT) No. of bitstreams: 1 lima_mag_me_rcla.pdf: 3177317 bytes, checksum: ef245c18ecc68d1f5e40173ca320aec4 (MD5) / Made available in DSpace on 2018-02-20T13:56:23Z (GMT). No. of bitstreams: 1 lima_mag_me_rcla.pdf: 3177317 bytes, checksum: ef245c18ecc68d1f5e40173ca320aec4 (MD5) Previous issue date: 2018-01-30 / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / O presente trabalho constitui-se em um estudo das potencialidades didático-pedagógicas do Laboratório de Ensino de Matemática (LEM), na visão de professores, como auxiliar na superação de dificuldades dos alunos do Ensino Médio, diante dos conceitos e dos procedimentos da Álgebra, em especial ao uso da linguagem simbólica. Em um primeiro momento, discorre-se sobre a diversidade de “conceitos” atribuídos ao termo Álgebra e sobre ideias envolvidas no termo LEM. Analisam-se dois documentos curriculares, levando-se em conta conceitos, procedimentos e conteúdos algébricos, sob o enfoque da linguagem simbólica. Elucidam-se alguns problemas enfrentados pelos alunos, ressaltando-se a questão problemática em torno da linguagem simbólica. A fim de ilustrar tal situação, apresentam-se relatos de professores, nos quais argumentam sobre a importância da Álgebra e comentam sobre dificuldades encontradas pelos alunos. Em etapa posterior, atingindo-se a proposta da pesquisa, seleciona-se – como fonte para análise de propostas de intervenção que contemplem a metodologia do LEM e os conteúdos algébricos do Ensino Médio – o XI Encontro Nacional de Educação Matemática, com o tema “Educação Matemática: Retrospectivas e Perspectivas”. Examinou-se uma amostra de trabalhos obtida junto ao site do evento, composta por duas modalidades de pesquisas: Comunicações Científicas e Relatos de Experiência. A pesquisa enquadra-se na abordagem qualitativa, sendo, portanto, de natureza bibliográfica. A seleção e análise da amostra permitiram a identificação de uma variedade de questões concernentes ao trabalho com atividades laboratoriais e a Álgebra do Ensino Médio, tais como: o baixo número de estudos encontrados; a falta de diversificação quanto à contemplação dos conteúdos algébricos, centrando-se no tema Funções; estudos com desenvolvimentos repetitivos, alguns deles apresentando, superficialmente, o conteúdo abordado; falta de vinculação entre o uso de materiais concretos e jogos e os conteúdos abordados, dentre outros. Apesar disso, a análise dos dados confirma que há aproximação entre LEM e Álgebra, permitindo que se argumente a favor do LEM que: (i) as atividades laboratoriais encontradas favorecem o domínio da linguagem simbólica, pois incentivam o registro em linguagem verbal, estimulam a generalização verbal como ponto de partida para a generalização simbólica e proporcionam a abstração por meio de atividades experimentais ou a abstração por meio da observação; (ii) as tendências em Educação Matemática envolvidas nas atividades, por exemplo, a Modelagem Matemática e a Resolução de Problemas, assim como recursos concretos e softwares matemáticos, promovem situações em que a notação usada aproxima-se da notação formal da Matemática; (iii) o LEM contribui para a superação de dificuldades em Álgebra do Ensino Médio, oportunizando o enfrentamento de deficiências de níveis escolares anteriores. Em geral, o presente trabalho indica dois pontos centrais de importância para a Educação Matemática: o reconhecimento da ligação entre o LEM e a Álgebra, especialmente a presença de linguagem algébrica em atividades laboratoriais, e o fato de tais atividades não serem devidamente reconhecidas pelos professores no que concerne ao caso da Álgebra do Ensino Médio, inferindo-se a necessidade de ampliação da participação de professores de Matemática da Educação Básica na criação de propostas de ensino no campo da Álgebra. / This present paper comprehends a study on the didactic and pedagogical potentialities of the Math Teaching Lab (LEM) as seen by teachers, as an aid for high school students to overcome their difficulties in the face of the concepts and processes in Algebra, especially concerning the use of symbolic language. To begin with, the diversity of “concepts” attributed to the term Algebra and the ideas around the term LEM are discussed. Two curricular documents are analyzed, taking into consideration algebraic concepts, processes and contents, under the focus of symbolic language. Some of the problems faced by students are elucidated, pinpointing the problematic matter around symbolic language. Aiming at illustrating such situations, reports by teachers are presented, in which they discuss the importance of Algebra and comment on the difficulties found by their students. At a following stage, upon fulfilling the research proposal, the XI Encontro Nacional de Educação Matemática (11th National Mathematical Education Conference) – with the theme “Mathematical Education: Retrospects and Prospects” – is chosen as a source of analysis of intervention proposals which observe LEM methodology and high school algebraic contents. A sample of works obtained from the website of the event, composed of two research categories: Science Communications and Experience Reports, was examined. The research fits a qualitative approach, therefore, it is of a bibliographic nature. The sample selection and analysis allowed the identification of a variety of questions concerning the work with lab activities and high school Algebra, such as: the small number of studies found; the lack of diversification in terms of comprehending algebraic contents, focusing on the Functions subject; studies with repetitive development, some of them presenting the discussed content superficially; the lack of linkage between the use of concrete material and games and the discussed content, among others. In spite of that, the data analysis confirms that there is an approach between LEM and Algebra, allowing arguing in favor of LEM that: (i) the lab activities found promote the dominance of symbolic language, for they motivate the record in verbal language, promote verbal generalization as a starting point to symbolic generalization and they provide abstraction by means of experimental activities or abstraction by means of observation; (ii) the tendencies in Mathematical Education involved in the activities, for example, Mathematical Modeling and Problem Solving, as well as concrete resources and mathematical software, promote situations in which the notation used is close to formal mathematical notation; (iii) LEM contributes to overcome difficulties in high school Algebra, providing the tackling of deficiencies of previous schooling stages. Overall, the present work indicates two important main points for Mathematical Education: the acknowledgement of a linkage between LEM and Algebra, especially the presence of algebraic language in lab activities, and the fact that these activities are not properly recognized by teachers, concerning Algebra in high school, what infers the need to increase the participation of elementary school Mathematics teachers in the creation of teaching proposals in the field of Algebra.

Submitted by MARCOS EDER CUPAIOLI (marcoscupaioli@hotmail.com) on 2016-12-02T01:24:31Z No. of bitstreams: 1 Dissertação ME Cupaioli pdf2.pdf: 1786404 bytes, checksum: db704a54f33dc6b28331ff0bbb07a982 (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-12-05T13:25:38Z (GMT) No. of bitstreams: 1 cupaioli_me_me_prud.pdf: 1786404 bytes, checksum: db704a54f33dc6b28331ff0bbb07a982 (MD5) / Made available in DSpace on 2016-12-05T13:25:38Z (GMT). No. of bitstreams: 1 cupaioli_me_me_prud.pdf: 1786404 bytes, checksum: db704a54f33dc6b28331ff0bbb07a982 (MD5) Previous issue date: 2016-09-22 / O presente trabalho aborda um conjunto de atividades experimentais com materiais de fácil manuseio, recicláveis e de baixo custo, baseados em uma abordagem de Teaching-Learning Sequence (TLS) ou Sequência de Ensino-Aprendizagem com enfoque intervencionista inspirada na investigação educacional. Utilizando para isso metodologias problematizadoras, visando o entrelaçamento científico e a perspectivas dos alunos sendo estes os protagonistas do seu conhecimento, fazendo a montagem e realizando as atividades experimentais, tendo o professor como mediador, orientando e explicando aos alunos em todo o processo. Além de abordar algumas atividades experimentais, mostrando como são realizadas as montagens dos experimentos e os objetivos a serem atingidos, trazemos um roteiro e uma sequência de como acreditamos que devemos abordá-las em sala de aula. Após a finalização dos experimentos, a análise dos resultados e a validação da aprendizagem, verificou-se, claramente, os progressos obtidos na aprendizagem da Física, com resultados bastante satisfatórios. As atividades aqui propostas podem ser utilizadas e redesenhadas pelo professor, de acordo com o nível de ensino e do conhecimento de seus alunos. / This study approaches a set of experimental activities with easy-handling, recyclable, low-cost materials, based on a Teaching-Learning Sequence (TLS) approach, with interventionist focus inspired by educational investigation. Using problematizing methodologies, aiming scientific intertwining and also the students’ perspective, with the students being the protagonists regarding their own knowledge creating and performing experimental activities, the teacher being a mediator, guiding and detailing with the whole process. Besides approaching some experimental activities, showing the way the experiments are prepared and the goals to be met, we bring guidelines and a sequence indicating how these activities should be approached in the classroom. After completion of the experiments, the analysis of the results and verification of learning, it was clearly verified progress acquired in learning physics with very satisfactory results. The activities proposed here can be used and adapted by teachers, according to the education level and knowledge of their students.

Orientador: José Jurandir Fagliari / Banca: Roberto Calderon Gonçalves / Banca: Julio Augusto Naylor Lisboa / Banca: Maria da Glória Buzinaro / Banca: Francisco Leydson Formiga Feitosa / Resumo: O presente estudo teve como objetivo avaliar as alterações clínicas e laboratoriais de bezerros infectados experimentalmente com Salmonella Dublin e verificar o efeito do tratamento com antibiótico florfenicol associado ou não à fluidoterapia. Para isso, foram constituídos quatro grupos experimentais, compostos por seis bezerros cada, que receberam, por via oral, aproximadamente 108 UFC de S. Dublin (exceto o grupo 1) e que foram submetidos aos seguintes procedimentos: controle (grupo 1), sem tratamento (grupo 2), tratamento apenas com florfenicol (grupo 3) e tratamento com florfenicol associado à fluidoterapia (grupo 4). Todos os bezerros foram submetidos ao exame físico antes da inoculação experimental e a cada 12 horas, até o sétimo dia após a infecção. Nestes momentos foram colhidas amostras de sangue, para realização de exames hematológicos, hemogasométricos, bioquímicos e proteinograma, e suabes retais, para isolamento de Salmonella por meio de exames microbiológicos e da reação em cadeia da polimerase (PCR). A infecção experimental com S. Dublin induziu diarréia, febre, desidratação e sinais respiratórios nos bezerros inoculados e provocou aumento da contagem do número de leucócitos, da concentração de uréia, fibrinogênio e proteínas de fase aguda, e redução do valor do pH, do excesso de base, da concentração de albumina, magnésio e ferro. Os bezerros que receberam tratamento mostraram boa recuperação clínica, sendo que o grupo tratado com antibiótico associado à fluidoterapia apresentou correção mais rápida e eficiente do equilíbrio hidro-eletrolítico. Quanto à detecção de Salmonella Dublin, o isolamento microbiológico mostrou-se superior à PCR, contudo o uso simultâneo das duas técnicas propiciou o diagnóstico de um maior número de amostras positivas. / Abstract: The aim of the study was to evaluate clinical and laboratorial changes in experimentally Salmonella Dublin-infected calves and the effect of florfenicol antibiotic treatment associated or not with fluid therapy. Four experimental groups, comprising six calves, were formed. With the exception of group 1, animals orally received about 108 CFU of S. Dublin and underwent the following procedures: control (group 1), without any treatment (group 2), treatment with florfenicol only (group 3), treatment with florfenicol associated with fluid therapy (group 4). All calves were submitted to physical examination before experimental inoculation and at every 12 hours up to the 7th day after infection. Blood samples were collected for hematological, hemogasometric, and biochemical analysis, coupled to rectal swabs for the isolation of Salmonella by standard microbiological techniques and polymerase chain reaction (PCR). S. Dublin experimental infection caused diarrhea, fever, dehydration and respiratory effects in inoculated calves; increased values of leukocytes, urea, fibrinogen and acute phase proteins; decreased rate of pH, base excess, albumin, magnesium and iron. Treated calves had a satisfactory clinical recovery. The group treated with antibiotics associated with fluid therapy had a faster and more efficient correction of hydro-electrolyte alterations. Although microbiological isolation was better than PCR in the detection of Salmonella, the simultaneous use of both techniques provides a diagnosis with more positive samples. / Doutor