Tez (Yüksek Lisans) - Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, 2009. / Kaynakça var.

Τα καρκινώματα του λάρυγγα και του λαρυγγοφάρυγγα αποτελούν τη συχνότερη κακοήθη νεοπλασία Κεφαλής & Τραχήλου. Οι προκαρκινικές βλάβες είναι ενδιάμεσοι σταθμοί της καρκινογένεσης, που καταλήγει στον καρκίνο. Παράλληλα με την καρκινογένεση συμβαίνει ενεργοποίηση κυττάρων του υποεπιθηλιακού στρώματος. Η χημειοπρόληψη είναι απόπειρα φαρμακολογικής ανακοπής της καρκινογένεσης. Ο πυρηνικός υποδοχέας PPARγ καταλήγει σε διαφοροποίηση αρκετών κυττάρων. Ο RXRα ετεροδιμερίζεται με τον PPARγ και άλλους πυρηνικούς υποδοχείς. Ο μεταγραφικός παράγοντας NF-κB ενισχύει την κυτταρική επιβίωση και φλεγμονή. Ο μεμβρανικός υποδοχέας EGFR κινητοποιεί μιτογόνες διαδικασίες. Η COX-2 συνθέτει προσταγλανδίνες. Κατά την παρούσα εργασία, μελετήθηκε με ανοσοϊστοχημεία σε τομές παραφίνης η έκφραση των πέντε μορίων στο φυσιολογικό λαρυγγικό επιθήλιο, προκαρκινικές βλάβες και καρκινώματα διαφόρων Grade, καθώς και το υποκείμενο στρώμα, σε ιστικά δείγματα λάρυγγα από 127 ασθενείς. Από τους PPARγ, NF-κB(υπομονάδα p65) και RXRα, που εμφάνισαν μεικτή υποκυττάρια εντόπιση, οι δύο πρώτοι αναγνωρίστηκαν κυρίως στο κυτταρόπλασμα και ο τρίτος στον πυρήνα. Οι EGFR και COX-2 παρουσίασαν μεμβρανική και κυτταροπλασματική ανοσοδραστικότητα αντίστοιχα. Η έκφραση του υποδοχέα PPARγ συσχετίστηκε θετικά με την καλή διαφοροποίηση των όγκων. Ο RXRα ελαττώθηκε κατά την καρκινογένεση. Τα επίπεδα των παραγόντων NF-κB, EGFR και COX-2 παρουσίασαν αύξηση στην καρκινογένεση. Η COX-2 συσχετίστηκε αρνητικά με το Grade των όγκων. Στις υπόλοιπες συσχετίσεις μελετήθηκε η συνέκφραση των παραγόντων σε κάθε ασθενή. Παρατηρήθηκε στο φυσιολογικό και προνεοπλασματικό επιθήλιο, θετική σχέση της COX-2 με τους υπόλοιπους παράγοντες. Επιβεβαιώθηκε η συνέργεια των PPARγ & RXRα, ως προς την πρόκληση διαφοροποίησης στα καρκινικά κύτταρα. Διαπιστώθηκε κατασταλτική επίδραση του PPARγ στην καρκινογόνο δράση του NF-κB. Αποκαλύφθηκε συνεργιστική δράση της COX-2 στην PPARγ-εξαρτώμενη διαφοροποίηση, οφειλόμενη πιθανόν σε προσφορά της 15d-PGJ2, από το ένζυμο στον πυρηνικό υποδοχέα. Κατά την εκτίμηση του μικροπεριβάλλοντος, αξιολογήθηκε η έκφραση σε συνάρτηση με την ύπαρξη καρκινώματος και το βαθμό διαφοροποίησής του. Οι μυοϊνοβλάστες που περιβάλλουν καρκινώματα (CAFs), παρουσιάζουν επαγωγή των RXRα, NF-κB και COX-2. Μάλιστα η υψηλή έκφραση COX-2 στους CAFs, συνδυάζεται με υψηλό Grade. Ενεργοποίηση στους CAFs, συνδέεται με υπερ-δραστήρια NF-κB σηματοδότηση στο καρκίνωμα. Ο NF-κB υπερεκφράζεται στα λεμφοκύτταρα που διηθούν τους όγκους (TILs). Επίσης τα μακροφάγα γύρω από νεοπλάσματα (TAMs), περιέχουν υψηλό PPARγ. Η χρήση αγωνιστών των PPARγ, RXRα, με παράλληλη αναστολή των NF-κB, EGFR και COX-2, θα είχε πιθανότατα ευνοϊκό αποτέλεσμα στην αναστροφή της λαρυγγικής καρκινογένεσης. Το χημειοπροληπτικό σχήμα, θα ήταν ωφέλιμο να τροποποιείται ύστερα από εξατομικευμένη αξιολόγηση του δικτύου των πέντε παραγόντων. / Carcinomas of the larynx and laryngopharynx are the most common malignancies of the Head&Neck. The precancerous lesions are mid-points of carcinogenesis, which results in cancer. Carcinogenesis is accompanied by reactive initiation of stromal cells. Chemoprevention pursues the arrest of carcinogenesis, by pharmacological means. PPARγ, a nuclear receptor, promotes cellular differentiation. The nuclear receptor RXRα partners with PPARγ or other members of the superfamily. The transcription factor NF-κB enhances cell survival and inflammation. The receptor EGFR receives growth signals. The enzyme COX-2 perpetuates inflammation by means of prostaglandin synthesis. The present study, utilizing paraffin section immunohistochemistry, assessed expression of the five molecules in normal laryngeal epithelium, precancerous lesions and carcinomas, along with their adjacent stroma. Clinical samples were derived from 127 patients who had undergone biopsy or laryngectomy. Of the three molecules demonstrating mixed subcellular presence, PPARγ and NF-κB(p65 subunit) localized more frequently in cytoplasm, whereas RXRα expression was mainly nuclear. EGFR and COX-2 staining patterns were membranous and cytoplasmic, respectively. PPARγ correlated with high tumor differentiation. RXRα was diminished in dysplasia and cancer. NF-κB, EGFR and COX-2, were upregulated as tumorigenesis progressed. COX-2 showed an inverse relationship with tumor Grade. The remaining correlations are based on coexpression analysis of the aforementioned factors. COX-2 was positively associated in normal and preneoplastic epithelia with the four regulating proteins. The data verify the synergistic effect of PPARγ and RXRα, regarding potentiation of neoplastic cell differentiation. Suppressive influence of PPARγ on NF-κB–mediated carcinogenesis was manifested. COX-2 overexpression was shown to reinforce the beneficial role of PPARγ in cell differentiation, probably due to production of the receptor agonist 15d-PGJ2. Subepithelial stroma was examined with regard to immunoreactivity in relation to the presence of invasion as well as to tumor Grade. Carcinoma Associated Myofibroblasts (CAFs), manifested upregulation of RXRα, NF-κB and COX-2, which mediate in concert angiogenesis, inflammation, and tumor spread. Intense COX-2 expression in CAFs, correlated with poor tumor differentiation. CAF activation was associated with intense NF-κB signalling in cancer cells. NF-κB was overexpressed in Tumor Infiltrating Lymphocytes (TILs). Finally, in Tumor Associated Macrophages (TAMs), PPARγ was induced. PPARγ, RXRα agonists, combined with NF-κB, EGFR and COX-2 inhibitors, would likely restrain laryngeal carcinogenesis. Subtle variations in the chemopreventive regimen, based on personalized molecular profiling, would hopefully achieve a patient-tailored therapeutic approach.

Die vorliegende retrospektive Studie untersuchte die lokale Rezidiv- und Überlebensrate von Patienten mit Plattenepithelkarzinomen in Abhängigkeit vom Randstatus und Resektionsabstand der Tumorresektion sowie von der postoperativen Therapie. Dabei wurden Daten von 351 Patienten mit Plattenepithelkarzinomen des Oropharynx, Hypopharynx und Larynx ausgewertet, welche zwischen 2005 und 2009 reseziert wurden. Der gesundheitliche Zustand der Patienten wurde anschließend bis Dezember 2011 beobachtet. Im Ergebnis konnte kein Einfluss des Randstatus und des Resektionsabstandes auf die lokale Rezidivrate nachgewiesen werden. Beide Faktoren hatten jedoch einen signifikanten Einfluss auf die Letalitätsrate. Die postoperative Therapie zeigte einen positiven Einfluss auf die lokale Rezidivrate. Sie konnte aber die negativen Prognosefaktoren, die eine Indikation dieser Therapie darstellen, bezüglich der Letalitätsrate nicht ausgleichen. Weiterhin war ein positiver Einfluss der postoperativen Therapie auf das rezidivfreie Überleben (DFS-disease-free survival) sowohl uni- als auch multivariat nachweisbar. Die Gesamtüberlebensrate (OS-overall survival) wurde signifikant positiv durch einen geringeren Randstatus und einen zunehmenden Resektionsabstand (ASR Klassifikation) beeinflusst. Das krankheitsspezifische Überleben (DSS-disease-specific survival) sank bei Auftreten eines lokalen Rezidivs. Aus diesen Ergebnissen kann geschlussfolgert werden, dass eine postoperative Therapie zur Kontrolle lokaler Rezidive von hoher Bedeutung ist. Da lokale Rezidive die Überlebensrate signifikant senken, hat die postoperative Therapie indirekt einen positiven Effekt auf das Überleben. Des Weiteren zeigt sich bei Patienten mit freien Resektionsrändern die höchste Überlebensrate, wobei diese mit zunehmendem Abstand des Karzinoms vom Resektionsrand steigt.

Darbo tikslas: įvertinti šunų gerklų edemų etiotropinius faktorius, taikomas diagnostikos priemones bei gydymo ypatumus. Darbo uždaviniai: 1. Apžvelgti literatūroje pateikiamus duomenis apie gerklų edemų šunims etiologiją. 2. Išanalizuoti patologinio proceso pagrindinius ir pagalbinius diagnozavimo metodus. 3. Išanalizuoti gerklų edemų gydymui taikomas priemones, pateikti patologinio proceso prevencijos būdus. Buvo renkami duomenys 2005–2012 m. pacientų, kurie sirgo ligomis, sukeliančiomis gerklų edemą. Nuo 2005–2008 m. analizuotos tik pacientų ligos istorijos, kuriems buvo diagnozuota gerklų edema (n=133), o nuo 2008–2012 m. – gyvūnai (n=163) tirti kartu su veterinarijos gydytojais, dalyvauta atliekant diagnostines procedūras bei paskiriant gydymą. Išnagrinėti gerklų edemos etiologiniai veiksniai, jų diagnozavimo ir gydymo būdai. Tyrimų rezultatai ir išvados: išanalizavus surinktus duomenis apie pacientus, sergančius ligomis, kurios iššaukia gerklų edemą, nustatyta, kad pagrindiniai etiologiniai veiksniai, sukėlę gerklų edemą šunims, buvo laringitas (54 proc. arba 159 atvejai, p>0,05), laringotracheitas (27 proc. arba 78 atvejai, p>0,05), trachėjos kolapsas (11 proc. arba 34 atvejai, p<0,05), brachicefalinis sindromas (6 proc. arba 19 atvejų, p<0,05), navikai gerklų srityje (1 proc. arba 4 atvejai, p>0,05), vabzdžių įgėlimai (0,3 proc. arba 1 atvejis, p>0,05) ir šunidžių kosulys (0,3 proc. arba 1 atvejis, p>0,05). Gerklų edema dažniau diagnozuota patinams (58 proc. arba 17... [toliau žr. visą tekstą] / The objective of the research–to understand factors about dogs laryngeal oedema, laryngeal oedema diagnostic and treatment. Task of the research: 1. To analyze information about dogs laryngeal edema in literature. 2. To identify pathological process main and supporting diagnostic procedures. 3. To analyze treatment and ways of prevention of the laryngeal edema in dogs. 2005–2012 years, the information was picked about pacients, who had diseases, which stimulate laryngeal edema, from 2005–2008 information was picked about patients who has diseases which stimulate laryngeal edema, and from 2008–2012, the pacients were researched with vet doctor near abay. Were identifyied 296 pacients and their causes and treatment of laryngeal edema. Results and conclusions: main etiology factors of laryngeal edema in dogs were: laringytis (54 percent or 159 cases, p>0,05), laringotracheitis (27 percent or 78 cases, p>0,05), collapse of trachea (11 percent. or 34 cases, p<0,05), brachicefalic syndrome (6 percent. or 19 cases, p<0,05), tumours (1 percent or 1 case, p>0,05), bite of insect (0,3 % or 1 case, p>0,05) and kennel cough (0,3 % or 1 case, p>0,05). laryngeal edema,was identified more in males (58 percent or 172 cases), than females (42 percent or 124 cases). The patient average age was from 5,2±1,17 years old. Laryngeal edema diagnosed more in pedigreed dogs (72 percent or 214 cases) than in hybrids (28 percent or 82 cases). Major pedigreed dogs had ilnesses, who were from 1 to 5... [to full text]

Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 115-119).

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Made available in DSpace on 2015-07-22T20:49:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-08-26 / Objetivo: esse estudo tem o objetivo de analisar comparativamente a concentração de ácido hialurônico em pregas vocais de ratas fêmeas, idosas e jovens, e em ratos machos jovens. Método: foram utilizadas pregas vocais de 13 ratas e 10 ratos, divididos em 3 grupos: 5 ratas fêmeas idosas, 8 ratas fêmeas jovens e 10 ratos machos jovens. A concentração tecidual do ácido hialurônico foi determinada por fluorescência. Resultado: foram encontradas as seguintes concentrações de ácido hialurônico em pregas vocais de acordo com os grupos: 581,67 ng/mg em ratas idosas, 1275,64 ng/mg em ratas jovens e 999,39 ng/mg em ratos machos jovens. A análise estatística mostrou diferença entre o grupo de ratas idosas e os grupos ratas jovens e ratos jovens. Conclusão: a prega vocal das ratas idosas possui uma concentração de ácido hialurônico menor que a concentração da prega vocal das ratas e dos ratos jovens. / Objective: this study analyzes hyaluronic acid concentration in vocal folds of adult rats comparing old female, young female and young male groups. Method: 13 female and 10 male rats were used divided in tree groups: 5 old female rats, 8 young female rats and 10 male young rats. The hyaluronic acid concentration was determined by fluorescence. Results: the following hyaluronic acid concentration by groups were found: 581,67 ng/mg in old female rats, 1275,64 ng/mg in young female rats and 999,39 ng/mg in young male rats. Statistical analysis showed difference among the old female group and the others young groups. Conclusions: the old female vocal fold has less hyaluronic acid concentration than the vocal fold of young male and female rats. / TEDE / BV UNIFESP: Teses e dissertações

A caracterização das patologias da laringe até o presente momento é predominantemente realizada de forma qualitativa. Alguns trabalhos recentes têm quantificados parâmetros acústicos no intuito de discriminar as diversas patologias. Este trabalho verifica os valores médios das diversas medidas acústicas da voz e as relaciona com as diferentes patologias. Desta forma, futuramente será possível utilizar estes valores para quantificar e acompanhar diferentes estágios do tratamento da patologia. O conjunto de valores médios compõem o que chamamos de perfil acústico. / Until now the characterization of larynx pathologies has been done in a subjective manner. Recently some researches have been conducted in order to quantify acoustic parameters mainly to discriminate the larynx pathologies. In this work several acoustic parameters of voice was evaluated and their correlation with the pathologies were established. In the future, would be possible to use these parameters to quantify and to follow the therapy procedures. These values constitute the acoustic profile of the pathologies.

Introdução: O prognóstico dos carcinomas epidermóides de laringe é limitado e a taxa de sobrevida em cinco anos é menor que 70%. A relação de características clínicas e epidemiológicas tem sido investigada na sobrevida de pacientes com tumores de laringe, mas pouco se conhece sobre o efeito dos polimorfismos genéticos no prognóstico da doença. Objetivo: Estudar o papel dos polimorfismos genéticos de genes relacionados aos processos de invasão e metástase (MMP1 e MMP3), de inflamação (Interleucina 2, Interleucina 6, LTA) e reparo de DNA(XRCC1) no prognóstico do carcinoma epidermóide de laringe. Material e métodos: Coorte com 170 pacientes com carcinoma epidermóide de laringe,confirmados por exame anátomo-patológico. Os casos tiveram origem em estudo caso-controle conduzido em cinco hospitais de São Paulo, um hospital em Porto Alegre e outro em Goiânia. As informações sobre o status vital dos pacientes foram levantadas dos prontuários médicos e dos bancos de óbitos municipais e estaduais. A extração do DNA das amostras de sangue dos pacientes foi realizada pelo Instituto de Medicina Tropical da USP e a genotipagem dos polimorfismos genéticos pela Fundação Hemocentro de Ribeirão Preto da Faculdade de Medicina da USP. Resultados: Os polimorfismos genéticos estudados (MMP1 1607, MMP1 -519,MMP3 -1171, IL2 -384, IL2 114, IL6 -174, LTA 252 e XRCC1) não apresentaram efeitos com significância estatística na sobrevida global ou específica pela doença quando analisados isoladamente. Para a sobrevida global, o consumo excessivo de álcool, em g/L/dia, reduziu a sobrevida dos pacientes (80-119 g/L/dia: hazard ratio(HR)=4,0 intervalos com 95% de confiança (IC95%)=1,10-14,53; _120 g/L/dia: HR=5,6 IC95%=1,71-18,24). No modelo de Cox múltiplo, quando ajustados pelo polimorfismo genético MMP3 -1171, a sobrevida piorou para esses pacientes (80-119 g/L/dia: HR=4,9 IC95%=1,07-22,91; _120 g/L/dia: HR=6,3 IC95%=1,49-26,84). Para a sobrevida específica pela doença, o estadiamento clínico IV reduziu a sobrevida dos pacientes (HR=3,5 IC95%=1,67-7,28). No modelo de Cox múltiplo,com ajuste pelos polimorfismos genéticos IL6 -174 e MMP1 1607, a sobrevidaespecífica pela doença piorou para esses pacientes (HR=4,7 IC95%=1,38-16,25).Conclusões: Na coorte examinada, somente três dos oito polimorfismos genéticos estudados relacionaram-se com a sobrevida global e específica pela doença, porém apenas alterando o efeito dos valores dos HR brutos dos fatores consumo de álcool e estadiamento clínico, respectivamente na sobrevida global e sobrevida específica pela doença. Isoladamente, nenhum polimorfismo genético estudado interferiu na sobrevida dos pacientes com câncer de laringe. / Introduction: The prognosis of laryngeal squamous cell carcinoma is limited and survival rate is lower than 70%. The relationships between clinical and epidemiological characteristics have been fully investigated on the survival of patients with laryngeal tumors, but the effect of genetics polymorphisms on squamous cell carcinoma of larynx is not well-known. Objective: To study the role of genetic polymorphisms of genes related to the processes of invasion and metastasis (MMP1 and MMP3), inflammation (Interleukin 2, Interleukin 6, and LTA) and repair of DNA (XRCC1) in the prognosis of laryngeal squamous cell carcinoma. Material and methods: Cohort with 170 laryngeal squamous cell carcinoma patients with histological confirmation. The cases have their origin in a case-control study carried out in hospitals of Sao Paulo, Porto Alegre and Goiania. The information about vital status of patients had been raised from medical records. The extraction of DNA was carried out by Institute of Tropical Medicine of USP and genotyping was carried out by the Center of Cellular Therapy of the Hemocentro of Ribeirao Preto of Medical School of USP. Results: The studied genetic polymorphisms (MMP1 1607, MMP1 -519, MMP3 -1171, IL2 -384, IL2 114, IL6 -174, LTA 252 and XRCC1), separately analyzed, did not have any statistical significant effect on the overall and cause-specific survival. High levels of alcohol consumption (g/L/day) reduced the overall survival (80-119 g/L/day: hazard ratio(HR)=4.0 intervals with 95% of confidence (95%CI)=1.10-14.53; _120 g/L/day:HR=5.6 95%CI=1.71-18.24). Multiple Cox model revealed, when adjusted for MMP3 -1171 genetic polymorphism, lower survival for those patients (80-119g/L/day: HR=4.9 95%CI=1.07-22.91; _120 g/L/day: HR=6.3 95%CI=1.49-26.84). The clinical staging (CS) IV was a factor for low cause-specific survival (CS IV:HR=3.5 95%CI 1.67-7.28). In the multiple Cox model, adjusted for genetic polymorphism IL6 -174 and MMP1 1607, the survival of those patients droppe(HR=4.7 95%CI=1.38-16.25). Conclusions: In this cohort, only three of eight genetic polymorphisms studied were showed to be related with overall and causespecific survival, however only modifying the effect of unadjusted HR of alcohol consumption and tumor clinical staging in the overall and cause-specific survival respectively. None of the studied genetic polymorphisms, when analyzed separately,affected the survival of laryngeal cancer patients.

Orientador: Plínio Almeida Barbosa / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Estudos da Linguagem / Made available in DSpace on 2018-08-17T08:58:39Z (GMT). No. of bitstreams: 1 Lima-Gregio_AvelinyMantovan_D.pdf: 2533583 bytes, checksum: 7d9212f2ef11e1b2e829368236a2934d (MD5) Previous issue date: 2011 / Resumo: Os objetivos deste estudo foram: 1) caracterizar a produção de oclusiva glotal de sujeitos com fissura palatina, a partir da percepção deste fenômeno pelos profissionais da área; 2) comparar as produções de cinco sujeitos sem fissura palatina (Grupo Controle - GC) com as de cinco sujeitos com fissura palatina (Grupo Teste - GT) na produção de laringalização em relação às regiões de fronteira; e 3) avançar no entendimento das articulações compensatórias (AC) e da teoria dos sistemas dinâmicos. O corpus consistiu na leitura de um texto realizada por ambos os grupos. A análise da oclusiva glotal foi realizada após a identificação de sua presença por cinco fonoaudiólogos experientes no diagnóstico e avaliação das AC e/ou disfunção velofaríngea (DVF). Em seguida, para cada produção foi observada a presença de três parâmetros (burst, formantes de transição e laringalização). Foi realizado um experimento de percepção, a partir da manipulação do sinal áudio de um participante do GC. Então, a análise prosódica da laringalização foi realizada. Quanto à análise da oclusiva glotal, os resultados mostraram que a presença de formantes de transição e burst foi determinante para a dúvida dos profissionais. Na ausência destes dois parâmetros, os juízes foram significativamente unânimes em afirmar a presença de oclusiva glotal. A presença de laringalização ajudou os fonoaudiólogos a julgarem a ocorrência da oclusiva glotal. A ocorrência de laringalização para o GC foi maior nas regiões de fronteira prosódica, enquanto que para o GT foi dentro e fora destas regiões. O experimento de percepção revelou um estímulo ambíguo, embora com significância estatística para concordância entre os juízes. A discussão foi norteada pelos pressupostos teóricos da Fonologia Articulatória, derivada do modelo de Browman e Goldstein (1990) e da Fonética Cognitiva de Tatham (1994). Discutiu-se ainda o estado da glote, a partir do conceito de válvulas laríngeas (Edmondson e Esling, 2006). A análise da oclusiva glotal evidenciou dois aspectos: a utilização de diferentes válvulas laríngeas e a proposta de um gesto glotal tripartido. A análise da laringalização sugere falha na supervisão do gesto glotal, o acoplamento da oclusiva glotal e da laringalização, e ação das fronteiras como atratores para as laringalizações / Abstract: The objectives of this study were: 1) to characterize the production of glottal stop of individuals with cleft palate, from the perception of this phenomenon by professionals; 2) to compare the productions of five subjects without cleft palate (Control Group - GC) and five subjects with cleft palate (Test Group - GT) to produce laryngealization in relation to border regions; and 3) improve the understanding of compensatory articulations (AC) and the theory of dynamical systems. The corpus consisted of the reading of the same text by both groups. The analysis of the glottal stop was made after identifying the presence by five speech pathologists (judges) experienced in the diagnosis and assessment of AC and/or velopharyngeal dysfunction (DVF). Then, for each production up to three parameters (burst, formant transition and laryngealization) were observed. A speech perception experiment was conducted after manipulating the audio signal of a participant in the GC. Then, the prosodic analysis of laryngealization was carried out. As regards the glottal stops, the results showed that the presence of burst and formant transition was responsible for ambiguous judgments. When these two parameters were absent, the judges were unanimous in signaling the presence of a glottal stop. The presence of laryngealization helped speech pathologists to identify glottal stop. For GC, the occurrence of laryngealization was in the prosodic boundary while for the GT laryngealization occurred everywhere. The speech perception experiment revealed an ambiguous stimulus, although a statistically significant correlation between the judges. The discussion was guided by theoretical assumptions of Articulatory Phonology, derived from the Browman and Goldstein's (1990) model and Tatham's (1994) Cognitive Phonetics. It was also discussed the state of the glottis, from the concept of laryngeal valves (Edmondson and Esling, 2006). Analysis of the glottal stop emphasizing two aspects: the use of different laryngeal valves and a proposed tripartite glottal gesture. The laryngealization analysis suggests failure of the supervisor component of the glottal gesture, the glottal stop and laryngealization coupling and the boundary regions as attractors for laryngealization / Doutorado / Linguistica / Doutor em Linguística