Made available in DSpace on 2016-04-28T14:57:34Z (GMT). No. of bitstreams: 1 2008 - Everaldo Hans Studt Klein.pdf: 5165097 bytes, checksum: ccfbfbc6baec84bc829dad886555adf9 (MD5) Previous issue date: 2008-07-15 / It was conducted a survey of the major diseases that occur in orchids in the State of Rio de Janeiro, through collection of plants in various localities in the period April 2006 to June 2008. Fifty-three plants had their diseases and pathogens identified, of which 35.9% were infected by fungi, being 17% by Fusarium oxysporum, 13.2% by Colletotrichum gloeosporioides, 1,9% by Botrytis sp., 1,9% by Puccinia sp. and 1.9% by Phyllosticta capitalensis. 51% of the plants were infected by virus with simple and double infections of Cymbidium mosaic virus - CymMV and Odontoglossum ringspot virus - ORSV. 1.9% were infected by the bacterium Pectobacterium carotovorum subsp. carotovorum and 3.8% by nematodes of the genus Aphelenchoides. The nematodes Aphelenchoides fragariae and A. ritzemabosi were identified infecting hybrid orchids, which is the first report of parasitism of these nematodes in orchid and of A. ritzemabosi parasiting Asplenium nidus L. in Brazil. It also made the first record of the occurrence of Cymbidium mosaic virus - CymMV infecting Bamboo orchid Arundina bambusifolia Lindl. in the State of Rio de Janeiro. Based on the identification of pathogens of that survey, it was developed a diagnostic kit for the main diseases of orchids. Aiming to test antimycotic properties of three substances in culture medium for cultivation of orchids, using as test- plants Sophronitis cernua Lindl. and Epidendrum ellipticum Grah. Of the substances tested, only powdered cinnamon (Cinnamomum zeylanicum J. Presl), at the dose of 6 grams / liter positively influenced the germination of seeds of Epidendrum ellipticum Graham, and the addition of clove (Syzygium aromaticum) at doses of 3, 5 and 6 grams / liter caused the inhibition of in vitro germination of seeds of Epidendrum ellipticum Graham. and Sophronitis cernua (Lindl.) Hook. The addition of commercial garlic (Allium sativum L.) powder in doses of 6 and 10 grams / litre, also inhibited in vitro germination of seeds of Epidendrum ellipticum Graham. / Foi realizado levantamento das principais doen?as que ocorrem em orqu?deas no Estado do Rio de Janeiro, atrav?s de coleta de plantas em v?rias Localidades, no per?odo de abril de 2006 a junho de 2008. Cinq?enta e tr?s plantas tiveram suas doen?as e pat?genos identificados, dos quais 35,9% das plantas estavam infectadas por fungos, sendo 17% por Fusarium oxysporum, 13,2% por Colletotrichum gloeosporioides, 1,9 % por Botrytis sp., 1,9% por Puccinia sp. e 1,9% por Phyllosticta capitalensis. 51% das plantas estavam infectadas por v?rus com infec??es simples e duplas de Cymbidium mosaic virus - CymMV e Odontoglossum ringspot virus - ORSV. 1,9 % estavam infectadas pela bact?ria Pectobacterium carotovorum subsp. carotovorum e 3,8 % por nemat?ides do g?nero Aphelenchoides. Foram identificados os nemat?ides Aphelenchoides fragariae e A. ritzemabosi infectando orqu?deas h?bridas, sendo esse o primeiro relato de parasitismo desses nemat?ides em orqu?dea e de A. ritzemabosi em Asplenium nidus L., no Brasil. Foi feito tamb?m o primeiro registro da ocorr?ncia Cymbidium mosaic virus CymMV infectando plantas da esp?cie Arundina bambusifolia Lindl. no Estado do Rio de Janeiro. Com base na identifica??o dos pat?genos desse levantamento, desenvolveu-se um kit diagn?stico para os principais fitopat?genos de orqu?dea. Objetivou-se testar tr?s subst?ncias de propriedades fungist?ticas em meio de cultivo de propaga??o de orqu?deas, utilizando-se como plantas-teste Sophronitis cernua Lindl. e Epidendrum ellipticum Grah.. Das subst?ncias testadas, apenas canela em p? (Cinnamomum zeylanicum J.Presl), na dosagem de 6 gramas / litro influenciou positivamente a germina??o de sementes de Epidendrum ellipticum Graham, sendo que o acr?scimo de cravo-da-?ndia (Syzygium aromaticum) comercial picado nas doses de 3, 5 e 6 g/l causou a inibi??o da germina??o in vitro das sementes de Epidendrum ellipticum Graham. e Sophronitis cernua (Lindl.) Hook. O acr?scimo de alho (Allium sativum L.) em p? comercial nas doses de 6 e 10 g/l, tamb?m inibiu a germina??o in vitro das sementes de Epidendrum ellipticum Graham.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-10-26T21:50:41Z No. of bitstreams: 1 KiviaSoaresDeOliveira_DISSERT.pdf: 1895024 bytes, checksum: 2a2bf1e0bc96f615985b29c1cf0e081d (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-12-26T16:48:53Z (GMT) No. of bitstreams: 1 KiviaSoaresDeOliveira_DISSERT.pdf: 1895024 bytes, checksum: 2a2bf1e0bc96f615985b29c1cf0e081d (MD5) / Made available in DSpace on 2016-12-26T16:48:53Z (GMT). No. of bitstreams: 1 KiviaSoaresDeOliveira_DISSERT.pdf: 1895024 bytes, checksum: 2a2bf1e0bc96f615985b29c1cf0e081d (MD5) Previous issue date: 2016-01-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A mangabeira ? uma frut?fera nativa do nordeste brasileiro e apresenta grande import?ncia socioecon?mica, ambiental e cultural. Apesar disso, vem sofrendo forte press?o negativa devido ? expans?o imobili?ria, desmatamento, pr?ticas de monoculturas e a explora??o extrativista n?o sustent?vel em seus campos nativos, o que tem ocasionado ? eros?o gen?tica da esp?cie, amea?ando-a de extin??o. Diante disso, ? iminente a necessidade de estudos que contribuam para a conserva??o da esp?cie tanto in situ como ex situ. Nesse contexto, o objetivo deste trabalho foi contribuir para o aprimoramento de estrat?gias de conserva??o da Mangabeira utilizando como ferramenta a biotecnologia vegetal e a percep??o ambiental. Como objetivos espec?ficos, buscou-se testar se a biotecnologia vegetal mensurada na utiliza??o de reguladores de crescimento, especificamente, BAP, IBA e ANA, pode contribuir para a conserva??o atrav?s da multiplica??o in vitro da mangabeira. E sob a perspectiva social, avaliar se a percep??o do extrator de mangaba ? afetada por caracter?sticas socioecon?micas (renda, idade, sexo, experi?ncia e escolaridade) e ambientais (regi?o que vive o extrator) e investigar se os extratores vegetais percebem a varia??o de abund?ncia apenas de esp?cies comerciais. Mediante a aplica??o de entrevistas semiestruturadas, foi constatado que os extrativistas vegetais das comunidades de Timb? e Boa ?gua, do munic?pio de N?sia Floresta- RN possuem conhecimentos sobre a esp?cie e que percebem a diminui??o desses recursos, bem como reconhecem a necessidade de conserva??o da esp?cie. Em rela??o ? utiliza??o da biotecnologia vegetal, foram obtidos resultados promissores para ? multiplica??o in vitro da esp?cie com a forma??o de brota??es bem desenvolvidas, permitindo uma excelente via de propaga??o, tendo em vista as dificuldades de reprodu??o natural da esp?cie. / The mangabeira (Hancornia speciosa Gomes) is a native fruit species from Brazil wich has a broad geographic distribution that goes from the Cerrado to the coastal plains of the Northeast, being well known for socio-economic, environmental and cultural importance. In the last decade the intensification of human actions has contributed to the significant reduction in their native fields, thus, to their genetic diversity. This justifies the imminent need for alternatives that enable its recovery in natural environment and the conservation of its genotype. Concomitantly, there is no effective awareness by the extractive communities concerning the need for environmental conservation of the resources and sustainable management. Accordingly, the study of environmental perception can contribute to the conservation of mangabeira in their occurrence areas because knowledge of extractive communities can enable a better understanding of sustainability and human activities operating in the region. Moreover, in view of the vulnerability of the species in the field, the plant biotechnology has become a promising alternative to solve this process and for species which are hard for natural propagation. Vegetative propagation in vitro, by enabling the production of plants on a large scale and plant quality, has helped in the conservation of many woody species. In this context, the objective was to contribute to the improvement of strategies for the conservation of H. speciosa using plant biotechnology as a tool and the environmental awareness. As specific objectives, we sought to test whether the plant biotechnology measured on the use of growth regulators (specifically: BAP, AIB and ANA), can contribute to the conservation by in vitro multiplication of mangabeira. And, for the social perspective, identify the socioeconomic and environmental awareness of plant extraction of two communities in N?sia Floresta - RN about the mangabeira, investigating whether the plant extractivists realize the change in abundance of commercial species only. For this, semistructured interviews were applied to the vegetable extractivists communities of Timb? and Boa ?gua, in the city of N?sia Floresta - RN. Regarding the knowledge of local communities to the use of the mangabeira is evident the preference and appreciation of its fruit as a native fruit to the region. The research showed that communities have knowledge regarding the species and that they perceive the reduction of their resources. Communities report the need for investments to facilitate the processing and marketing of fruits and their life quality. Regarding the use of plant biotechnology, it brought favorable results for the production of seedlings of the species, with the formation of more vigorous shoots.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-07-20T00:37:17Z No. of bitstreams: 1 Tese_final_ MARCHI.MNG.pdf: 2477043 bytes, checksum: 875c56fb9a49e8316ee438fe52b80636 (MD5) / Made available in DSpace on 2016-07-20T00:37:17Z (GMT). No. of bitstreams: 1 Tese_final_ MARCHI.MNG.pdf: 2477043 bytes, checksum: 875c56fb9a49e8316ee438fe52b80636 (MD5) Previous issue date: 2016-05-04 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The family Cactaceae has approximately 100 genera and 1500 species predominantly American and distributed by a wide variety of climates and ecosystems. Due to the high singularity in terms of genres and endemic species as well as the potential use of these plants for various purposes, studies on the in vitro propagation and ex situ conservation of these species must be established. Thus, the aim of this study was to understand the anatomical, physiological and molecular changes associated with the propagation and conservation in vitro Discocactus zehntneri subsp boomianus and Stephanocereus leutezlburgii, species endemic cactus of Bahia with ornamental potential. The regulators applied not exceeded the control in the induction of shoots/explant in both species and for S. luetzelburgii combination of cytokinins and auxins hiperidricos increased the percentage of shoots that did not survive after acclimatization. Normal shoots of this species have 100% survival in ex vitro conditions. The callus formation was enhanced by adding auxin to the nutrient medium for the two species analyzed and calluses with potential for in vitro morphogenesis were obtained. The proteomic analysis revealed the marker proteins differential expression of somatic embryogenesis in two strains of S. luetetzelburgii calluses. The protocol for cryopreservation D. zehntneri shoots was not effective, however the seed storage either in liquid nitrogen conditions as for ultrafreezer maintained physiological quality after storage for 360 and 180 days, respectively. Osmotic agents were effective in inhibiting in vitro growth of the species for a year without loss of viability. / A fam?lia Cactaceae apresenta cerca de 100 g?neros e 1500 esp?cies predominantemente americanas e distribu?das por uma ampla diversidade de climas e ecossistemas. Devido ? alta singularidade em termos de g?neros e esp?cies end?micas bem como o uso potencial destas plantas para diversas finalidades, estudos acerca da propaga??o in vitro e conserva??o ex situ dessas esp?cies devem ser estabelecidos. Desta forma, o objetivo geral deste trabalho foi compreender as mudan?as anat?micas, fisiol?gicas e moleculares associadas ? propaga??o e conserva??o in vitro de Discocactus zehntneri subsp boomianus e Stephanocereus leutezlburgii, esp?cies de cact?ceas end?micas da Bahia com potencial ornamental. Os reguladores aplicados n?o superaram o controle na indu??o de brotos/explante em ambas as esp?cies e para S. luetzelburgii a combina??o de citocininas e auxinas aumentaram o percentual de brotos hiperidricos que n?o sobreviveram ap?s a aclimatiza??o. Brotos normais dessa esp?cie apresentaram 100% de sobreviv?ncia em condi??es ex vitro. A calog?nese foi potencializada pela adi??o de auxinas ao meio nutritivo para as duas esp?cies analisadas e calos com potencial para morfog?nese in vitro foram obtidos. A an?lise prote?mica revelou a express?o diferencial de prote?nas marcadoras da embriog?nese som?tica em duas linhagens de calos de S. luetetzelburgii. O protocolo para criopreserva??o de brotos de D. zehntneri n?o foi eficiente, no entanto a armazenamento de sementes tanto em condi??es de nitrog?nio l?quido quanto em ultrafreezer mantiveram a qualidade fisiol?gica ap?s o armazenamento por 360 e 180 dias, respectivamente. Os agentes osm?ticos foram eficientes em inibir o crescimento in vitro da esp?cie por um ano sem perda na viabilidade.

Made available in DSpace on 2014-12-17T15:54:52Z (GMT). No. of bitstreams: 1 MarianneMAC.pdf: 690557 bytes, checksum: b90b415abf3acc2f75d29ad4843a4cc8 (MD5) Previous issue date: 2009-02-27 / This work focuses the familiar cajuculture at Serra of Mel (RN) that presents a geographic and climatic structure favourable to the development of the cajuculture; the interaction between the ambient factors and agriculture in the region provides a propitious environment to the culture of the cashew; the objective of present work was associate ambient, economic, social and cultural factors of this municipy with the possibility of a sustainable agriculture associated with the vegetal biotechnology / Este trabalho enfoca a cajucultura familiar no munic?pio de Serra do Mel (RN), o qual apresenta uma estrutura geogr?fica e clim?tica favor?vel ? cajucultura; a intera??o entre os fatores ambientais e a agricultura na regi?o proporciona um ambiente prop?cio ao cultivo do cajueiro; buscou-se com o presente trabalho associar os fatores ambientais, econ?micos, sociais e culturais do munic?pio com a possibilidade de desenvolvimento de uma agricultura sustent?vel integrada ? biotecnologia vegetal

Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-07-15T14:46:30Z No. of bitstreams: 1 karin_frichis_do_nascimento.pdf: 1481669 bytes, checksum: c542b6f2d89d5f67694bf36d33eecd30 (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: on 2016-07-15T20:04:35Z (GMT) / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-07-15T20:19:47Z No. of bitstreams: 1 karin_frichis_do_nascimento.pdf: 1481669 bytes, checksum: c542b6f2d89d5f67694bf36d33eecd30 (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: rever: Refer?ncia ABNT Tipo: opem Acess Nota de disserta??o/Tese: colocar ano Palavra Chave: cada uma em um campo, sem pontua??o Ag?ncia Financiadora: Sempre por extenso e com a abrevia??o entre par?nteses no final on 2016-07-18T14:41:49Z (GMT) / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-07-19T12:58:18Z No. of bitstreams: 1 karin_frichis_do_nascimento.pdf: 1481669 bytes, checksum: c542b6f2d89d5f67694bf36d33eecd30 (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: Rever: Refer?ncia ABNT Tipo Nota de disserta??o/Tese Resumos Palavra Chave Ag?ncia Financiadora Seguir modelo: http://acervo.ufvjm.edu.br/jspui/handle/1/360 on 2016-07-21T15:22:35Z (GMT) / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-08-25T11:45:56Z No. of bitstreams: 1 karin_frichis_do_nascimento.pdf: 1481669 bytes, checksum: c542b6f2d89d5f67694bf36d33eecd30 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-08-25T12:22:54Z (GMT) No. of bitstreams: 1 karin_frichis_do_nascimento.pdf: 1481669 bytes, checksum: c542b6f2d89d5f67694bf36d33eecd30 (MD5) / Made available in DSpace on 2016-08-25T12:22:54Z (GMT). No. of bitstreams: 1 karin_frichis_do_nascimento.pdf: 1481669 bytes, checksum: c542b6f2d89d5f67694bf36d33eecd30 (MD5) Previous issue date: 2015 / O trabalho teve como objetivo desenvolver procedimentos de micropropaga??o para a esp?cie pau-terra-liso (Qualea multiflora Mart.) a partir de sementes germinadas in vitro e avaliar a emerg?ncia, o crescimento inicial e a sobreviv?ncia de mudas em fun??o de diferentes substratos e ambientes, em condi??es de viveiro. No primeiro cap?tulo, as sementes de Qualea multiflora foram submetidas ? desinfesta??o com hipoclorito de s?dio em diferentes concentra??es e tempos de imers?o para a sua introdu??o in vitro. Foi avaliado o percentual de germina??o e contamina??o. Utilizando o melhor tratamento do experimento de desinfesta??o, foi instalado outro experimento para comparar as composi??es distintas de meio de cultura MS (Murashige & Skoog, 1962) e WPM (Lloyd & McCown, 1981) na germina??o de pau-terra-liso. Avaliou-se o percentual de germina??o e de pl?ntulas normais. Na fase de multiplica??o foram utilizados dois tipos de explantes (segmento nodal e segmento cotiledonar) retirados das pl?ntulas germinadas in vitro que foram inoculados em meio de cultura WPM. Este foi suplementado com BAP em concentra??es diferenciadas e ANA. A fase foi constitu?da pelo cultivo inicial e dois subcultivos. Avaliaram-se os n?meros de brota??es por explantes e a altura da maior brota??o. Constatou-se que a concentra??o de 5% de hipoclorito de s?dio durante 20 minutos de imers?o proporcionou os melhores resultados de desinfesta??o e germina??o in vitro. Observou-se que o tipo de meio de cultura e a concentra??o influenciam na germina??o e na qualidade das pl?ntulas de Qualea multiflora, logo, recomenda-se o meio WPM com 100% de sais e vitaminas para essa esp?cie. Os melhores resultados de multiplica??o foram alcan?ados utilizando o explante cotiledonar e a concentra??o de 0,6 mg L-? de BAP. No cap?tulo 2, os experimentos foram instalados em ambiente de casa de sombra e de casa de vegeta??o utilizando quatro tipos de substratos, sendo: 1) 100% substrato comercial Bioplant?, 2) 70% de vermiculita de granulometria m?dia + 30% de fibra de coco, 3) 70% de vermiculita + 30% Bioplant?, e 4) 40% de vermiculita + 30% de fibra de coco + 30% de Bioplant?. Realizaram-se avalia??es aos 60, 90, 120 e 150 dias para verificar a emerg?ncia, o crescimento em altura e di?metro e a sobreviv?ncia das mudas. No final do experimento, foram obtidos o peso da mat?ria seca da xii parte a?rea, o peso de mat?ria seca de ra?zes, o peso de mat?ria seca total e a rela??o peso de mat?ria seca da parte a?rea e peso de mat?ria seca das ra?zes. Em casa de vegeta??o a emerg?ncia de Qualea multiflora obteve os maiores percentuais com o uso do substrato VB (70% de vermiculita + 30% de Bioplant ?). N?o ocorreu diferen?a no crescimento em altura entre as mudas que estavam em casa de vegeta??o e em casa de sombra. Para o ambiente casa de sombra, n?o houve diferen?as significativas entre as caracter?sticas de mat?ria seca analisadas, em fun??o dos substratos. Com os dados de sobreviv?ncia nos dois ambientes, conclui-se que a Qualea multiflora ? de dif?cil propaga??o em condi??es de viveiro, sendo necess?rios mais estudos para a produ??o de mudas da esp?cie. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / The study aimed to develop micropropagation procedures for the ?pau-terra-liso? species (Qualea multiflora Mart.) from seeds germinated in vitro and evaluate the emergency, the initial growth and survival of seedlings for different substrates and environments in arboretum conditions. In the first chapter, the Qualea multiflora seeds were subjected to disinfection with sodium hypochlorite in different concentrations and immersion times for its introduction in vitro. The percentage of germination and contamination was evaluated. Using the best treatment of disinfestation experiment, it was installed another experiment to compare the different compositions of MS (Murashige and Skoog, 1962) and WPM (Lloyd & McCown, 1981) on the ?pau-terra-liso? germination. It was evaluated the percentage of germination and normal seedlings. In the multiplication phase it was used two types of explants (nodal segments and cotyledon segment) taken from seedlings germinated in vitro which were inoculated in WPM culture. This one was supplemented with ?BAP? and ?ANA? in different concentrations. The stage was set for the initial culture and two subcultures. It was evaluated the shoot numbers per explant and the height of the larger shoot. It was found that the concentration of 5% sodium hypochlorite for 20 minutes immersion gave the best results of disinfestation and in vitro germination. It was observed that the type of culture environment and the concentration influence the germination and quality of seedlings of Qualea multiflora, so it is recommended a WPM environment with 100% salts and vitamins for this species. The best multiplication results were achieved using cotyledon explants and concentration of 0.6 mg L-? of BAP. In Chapter 2, the experiments were conducted in shade house and greenhouse environment using four types of substrates, as follows: 1) 100% commercial substrate Bioplant?, 2) 70% of average grain size of vermiculite + 30% coconut fiber, 3) 70% of vermiculite + 30% Bioplant?, and 4) 40% of vermiculite and 30% coconut fiber + 30% Bioplant?. Evaluations were performed at 60, 90, 120 and 150 days to verify the emergence, growth in height and diameter and survival of seedlings. At the end of the experiment, it was obtained the dry matter weight of the aerial part, the dry matter weight of the roots, the entire dry matter weight and the relation between the weight of dry matter of aerial part and weight xiv of dry matter of the roots. In a greenhouse, the emergency Qualea multiflora obtained the highest percentages with the use of VB substrate (70% vermiculite and 30% of Bioplant ?). There was no difference in height growth among the seedlings that were in the greenhouse and in the shade house. To the environment shade house, there were no significant differences among the characteristics of the analyzed dry matter, according to the substrates. With the survival data in both environments, it is concluded that the Qualea multiflora is difficult to spread in arboretum conditions; further research is needed for the production of seedlings of the species.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-07-09T19:37:57Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) clara_almeida_guerra.pdf: 1004414 bytes, checksum: 6688d13a6216ca1b6380300e85cdd209 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-07-18T13:16:18Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) clara_almeida_guerra.pdf: 1004414 bytes, checksum: 6688d13a6216ca1b6380300e85cdd209 (MD5) / Made available in DSpace on 2018-07-18T13:16:18Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) clara_almeida_guerra.pdf: 1004414 bytes, checksum: 6688d13a6216ca1b6380300e85cdd209 (MD5) Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O objetivo deste trabalho foi desenvolver uma metodologia de propaga??o in vitro de Lychnophora pohlii Sch. Bip., envolvendo as etapas de alongamento, enraizamento e pr?-aclimata??o de pl?ntulas cultivadas in vitro. Na etapa de alongamento, no primeiro experimento avaliou-se o efeito dos reguladores de crescimento ANA + BAP, ANA + TDZ e GA3 combinados com os meios MS e MS/2 na altura m?dia e percentual de calos. Ainda nessa etapa, no experimento dois e tr?s, utilizou-se o meio MS e concentra??es de GA3 (0; 0,5; 1; 2 e 4 mg L-1) e ANA (0,1; 0,3; 0,6; e 0,9 mg L-1), e avaliou-se a altura m?dia, percentual de enraizamento, n?mero m?dio de ra?zes e percentual de calos. Na etapa de enraizamento, nos experimento quatro e cinco foram testadas concentra??es de ANA e AIB (1,0 e 2 mg L-1) e quatro tempos de perman?ncia dos explantes no meio (7, 14, 21e 28 dias) e as avalia??es foram realizadas semanalmente quanto ao percentual de enraizamento, n?mero m?dio de raiz, altura m?dia e percentual de calos. Tamb?m nessa etapa, para os experimentos seis e sete, os tratamentos foram constitu?dos de dois tipos de meio (MS/2 e WPM/2) e concentra??es de ANA e AIB (0,1 e 0,5 mg L-1) e avaliados o percentual de enraizamento, n?mero m?dio de ra?zes, altura m?dia e percentual de calos. Na etapa de pr?-aclimata??o, as pl?ntulas foram transplantadas para copos pl?sticos (200 cm3) contendo vermiculita? autoclavada e os tratamentos foram constitu?dos por pl?ntulas cobertas com saco de polietileno intacto, pl?ntulas cobertas com saco de polietileno perfurado e aus?ncia de cobertura, onde se avaliou o percentual de sobreviv?ncia, altura m?dia, comprimento m?dio da raiz principal, mat?ria fresca da parte a?rea, mat?ria fresca da raiz, mat?ria seca da parte a?rea e mat?ria seca da raiz. Na fase de alongamento, a maior m?dia de altura foi verificada em explantes cultivados em meio MS, suplementado com ANA + BAP. A combina??o de ANA + TDZ aumentou o percentual de calo. As concentra??es de 0,9 e 0,1 mg L-1 proporcionaram a maior e menor n?mero m?dio de raiz por tratamento. O menor percentual de calo foi observado na concentra??o de 0,1 mg L-1 de ANA. Na fase de enraizamento a concentra??o de 1,0 mg L-1 de ANA proporcionou maior m?dia de altura e menor percentual de calo e na concentra??o de 2,0 mg L-1 se observou a menor m?dia de altura e o maior percentual de calo. O tempo de perman?ncia de 21 dias possibilitou a maior m?dia de altura dos explantes tratados com ANA e AIB. A concentra??o de 2,0 mg L-1 de AIB contribuiu com o maior n?mero m?dio de ra?zes. O meio WPM/2 suplementado com 0,5 mg L-1 de ANA obteve o maior n?mero m?dio de ra?zes. A maior m?dia para a altura foi encontrada nos explantes cultivados em meio WPM/2. A concentra??o de 0,1 mg L-1 de ANA proporcionou o menor percentual de calo. A concentra??o de 0,5 mg L-1 de AIB proporcionou os maiores valores para o percentual de enraizamento, n?mero m?dio de ra?zes e percentual de calo. Na fase de pr?-aclimata??o, o maior percentual de sobreviv?ncia foi observado no tratamento em que as pl?ntulas foram cobertas com sacos de polietileno intacto. As pl?ntulas cobertas com sacos de polietileno perfurado expressaram maior altura m?dia, comprimento m?dio da raiz, mat?ria fresca e seca da parte a?rea. Nessas condi??es os explantes mostraram-se responsivos quanto aos tratamentos testados, denotando boas perspectivas quanto ao cultivo in vitro dessa esp?cie. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The objective of this work was to develop an in vitro propagation methodology of Lychnophora pohlii Sch. Bip. including the stretching, rooting and pre-acclimatization of seedlings grown in vitro stages. In the stretching stage, the effect of the growth regulators ANA + BAP, ANA + TDZ and GA3 combined with the MS and MS/2 media were evaluated at the mean height and callus percentage. At this stage, the effect of concentrations of GA3 (0, 0.5, 1, 2 and 4 mg L-1) and ANA (0.1, 0.3, 0, 6, and 0.9 mg L-1) were evaluated at the mean height, rooting percentage, mean number of roots and percentage of callus. In the rooting stage, ANA and IBA concentrations (1.0 and 2 mg L-1) and four dwell times of the explants in the medium (7, 14, 21 and 28 days) were tested and rooting percentage, root mean number, mean height and callus percentage were evaluated weekly. Also in this step, the treatments were composed of two types of medium (MS/2 and WPM/2) and ANA and IBA concentrations (0.1 and 0.5 mg L-1). In the pre-acclimation stage, the seedlings were transplanted to plastic cups (200 cm3) containing autoclaved vermiculite?. The treatments were composed by seedlings covered with intact polyethylene bag, seedlings covered with perforated polyethylene bag and seedling uncovered It was evaluated the percentage of survival, mean height, mean length of the main root, fresh shoot matter, fresh root matter, shoot dry matter and root dry matter. In the elongation phase, the highest mean height was verified in explants grown in MS medium, supplemented with ANA + BAP. The combination of ANA + TDZ increased the callus percentage. Concentrations of 0.9 and 0.1 mg L-1 provided the highest and lowest root mean number per treatment, respectively. The lowest percentage of callus was observed for the concentration of 0.1 mg L-1 of ANA. In the rooting stage, the concentration of 1.0 mg L-1 of ANA provided a higher average height and a lower percentage of callus. At the concentration of 2.0 mg L-1, the lowest mean height and the highest percentage of callus were observed. The dwell time of 21 days allowed the highest average height of the explants treated with ANA and AIB. The concentration of 2.0 mg L-1 of AIB contributed with the highest average number of roots. The WPM/2 medium supplemented with 0.5 mg L-1 of ANA obtained the highest mean number of roots. The highest mean height was found in the explants grown on WPM/2 medium. The concentration of 0.1 mg L-1 of ANA provided the lowest percentage of callus. The concentration of 0.5 mg L-1 of IBA provided the highest values for rooting percentage, mean number of roots and percentage of callus. In the pre-acclimatization stage, the highest percentage of survival was observed in the treatment in which the seedlings were covered with bags of intact polyethylene. Seedlings covered with perforated polythene bags expressed higher mean height, root mean length, fresh and dry shoot matter. In these conditions, the explants were responsive to the treatments tested, indicating good perspectives regarding the in vitro cultivation of this species.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-28T21:50:56Z No. of bitstreams: 1 Disserta??o_Jessica_Coelho_Valerianoatual.pdf: 1739335 bytes, checksum: 6ad29bd975f2995c8c9a68ceee755a5b (MD5) / Made available in DSpace on 2016-09-28T21:50:56Z (GMT). No. of bitstreams: 1 Disserta??o_Jessica_Coelho_Valerianoatual.pdf: 1739335 bytes, checksum: 6ad29bd975f2995c8c9a68ceee755a5b (MD5) Previous issue date: 2016-02-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Schinopsis brasiliensis Engl. and Pseudobombax simplicifolium A. Robins are species widely distributed in the Brazilian semiarid. The pressure on the genetic resources from Caatinga biome, mainly medicinal and for wood, caused by subsistence or commercial use, leads to reduce genetic variability of these species. Based on that, the cytogenetic and the tissue culture can support the characterization and propagation strategies of their populations. The research goals of this study were evaluate growth regulators and culture media effects on the establishment and in vitro micropropagation for the species, and the use of fluorochromes CMA3/DAPI in the cytogenetic characterization of P. simplicifolium. For P. simplicifolium it was evaluated different DKW ingredient concentration, added or not activated charcoal and different concentration of BAP and IBA on the establishment and in vitro multiplication of the species. For S. brasiliensis, it was evaluated the micropropagation media DKW and WPM (with different concentration of BAP and IBA) and the use of activated charcoal and polyvinylpyrrolidone to reduce the oxidation during establishment of in vitro cultivation of the species. In S. brasiliensis, the addition of BAP in the micropropagation media did not promote in vitro multiplication of the species. In P. simplicifolium, the results obtained for variables studied varied depending on the regulators concentrations and among genotypes. Concentrations above 4.92 and 6.64 ?M.L-1 AIB inhibited the multiplication in vitro in both species. The double staining CMA3/DAPI allowed the visualization of 2n = 84 chromosomes and different numbers of CMA+ blocks in the genotypes of P. simplicifolium. / As esp?cies Schinopsis brasiliensis Engl. e Pseudocombax simplicifolium A. Robins encontram-se amplamente distribu?das no semi?rido brasileiro. A press?o sobre os recursos gen?ticos do bioma caatinga, principalmente, medicinal e madeireiro, seja devido ao uso para subsist?ncia ou comercial, tem levado ? perda da variabilidade gen?tica destas esp?cies. Em vista a isto, a cultura de tecidos e a citogen?tica podem auxiliar nas estrat?gias de caracteriza??o e propaga??o de suas popula??es. O objetivo deste trabalho foi avaliar o efeito de reguladores de crescimento e meios de cultura no estabelecimento e multiplica??o in vitro das duas esp?cies, bem como o uso dos fluorocromos CMA3/DAPI na caracteriza??o citogen?tica do imbiru?u. Em P. simplicifolium, avaliou-se diferentes concentra??es dos sais DKW, acrescidos ou n?o com carv?o ativado e diferentes concentra??es de BAP e AIB no estabelecimento e multiplica??o da esp?cie. Em S. brasiliensis, avaliou-se os meios DKW e WPM e o uso do carv?o ativado e da polivinilpirrolidona na redu??o da oxida??o, e o efeito de diferentes concentra??es de BAP e AIB no meio WPM no estabelecimento do cultivo in vitro da esp?cie. Em S. brasiliensis, a adi??o de BAP ao meio de cultura n?o favoreceu a multiplica??o in vitro da esp?cie. Em P. simplicifolium as respostas para as vari?veis estudadas variaram em fun??o das concentra??es dos reguladores e entre os gen?tipos da esp?cie. Concentra??es acima de 4,92 e 6,64 ?M.L-1 de AIB inibiram a multiplica??o in vitro em ambas esp?cies. A dupla colora??o CMA3/DAPI permitiu a visualiza??o de 2n= 84 cromossomos e diferentes n?meros de blocos CMA+ nos gen?tipos estudados de P. simplicifolium.

Made available in DSpace on 2014-12-17T15:54:53Z (GMT). No. of bitstreams: 1 AnaKOA_DISSERT.pdf: 1986200 bytes, checksum: d86826aeca9a3190905fe12b68562cd9 (MD5) Previous issue date: 2010-02-25 / The anthropical action caused destruction of great part of the Atlantic forest remaining today around 7% to 8% of the original portion. A classical example of the degradation is the situation of the Pau-Brasil (Caesalpinia echinata Lam.) which appellant exploration summarized abruptly decreased the wild occurrence of the species. Besides the economic exploration and the threat of extinction, the plant shows some characteristics that its own physiology makes difficult the survival in natural conditions. For this reason, any strategy developed to conserve it should also guarantee ways for its multiplication. In these conditions, the only reasonable technology is micropropagation in vitro based on immature segments of Catullus (explants or internodes). However, in virtue of the threat of extinction, extractivism and fenology, the disponibility of the sources of explants are reduced. Then, to turn this around, the present research showed the hypothesis that arrange these explants would have as a solution to elaborate a bank of matrixes and the utilization of produced seedlings as source of explant. Then, assuming that the soil is a fundamental element for a good formation of the seedlings, there were tests in a greenhouse on the Laboratory of plant biotechnology at UFRN, from May to June of 2008 that showed the influence of the four different soils on the production of Pau-Brasil. The objective of this work was to make possible the seedlings production of the specie and from them to establish a bank of matrixes as source of explant to guarantee the continuity of the process of micropropagation, contributing with the preservation of the specie / A a??o antr?pica causou a destrui??o de grande parte da Mata Atl?ntica restando hoje apenas cerca de 7% a 8% da sua por??o original. Um exemplo cl?ssico dessa degrada??o ? a situa??o do Pau-Brasil (Caesalpinia echinata Lam.) cuja explora??o recorrente resumiu drasticamente a ocorr?ncia silvestre da esp?cie. Al?m da explora??o econ?mica e da amea?ada de extin??o, a planta apresenta ainda caracter?sticas de sua pr?pria fisiologia que dificultam a sobreviv?ncia em condi??es naturais. Por esse motivo, qualquer estrat?gia desenvolvida para conserv?-la deve tamb?m garantir meios para sua multiplica??o. Nessas condi??es, a ?nica tecnologia plaus?vel ? a micropropaga??o in vitro a partir de segmentos imaturos de caule (os explantes). Entretanto, em virtude da amea?a de extin??o, do extrativismo e da fenologia, a disponibilidade das fontes de explante ? reduzida. Ent?o, para contornar esse contratempo, a presente pesquisa trabalhou a hip?tese de que para dispor desses explantes a solu??o seria elaborar um banco de matrizes e utilizar as mudas produzidas como fonte de explante. Ent?o, partindo do pressuposto de que o substrato ? um elemento fundamental para boa forma??o de mudas, testou-se em casa de vegeta??o do Laborat?rio de Biotecnologia Vegetal da UFRN, no per?odo de maio a junho de 2008, a influ?ncia de quatro diferentes substratos sobre a produ??o plantas de Pau-Brasil. O objetivo aqui foi viabilizar a produ??o de mudas da esp?cie e a partir das mesmas estabelecer um matrizeiro como fonte de explante para garantir a continuidade do processo de micropropaga??o, contribuindo assim com a preserva??o da esp?cie

Submitted by Luis Ricardo Andrade da Silva (lrasilva@uefs.br) on 2015-12-02T20:10:10Z No. of bitstreams: 1 v.final DISSERTA??O B?RBARA PAULA DOS SANTOS BORGES DOS SANTOS BORGES.pdf: 1253496 bytes, checksum: b385afcd4779d54bd2a324ab4f1f5b5f (MD5) / Made available in DSpace on 2015-12-02T20:10:10Z (GMT). No. of bitstreams: 1 v.final DISSERTA??O B?RBARA PAULA DOS SANTOS BORGES DOS SANTOS BORGES.pdf: 1253496 bytes, checksum: b385afcd4779d54bd2a324ab4f1f5b5f (MD5) Previous issue date: 2015-08-26 / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / Vellozia sincorana Ayensu & SMITHis endemic of Rupestres Fields of Chapada Diamantina, popularly known as Candomb?. This species has a highly flammable resin, and the stem used to ignite wood stoves and roots to produce incense. Candomb? presents few studies in the literature, there are no studies of propagation of the species. This study aimed to develop in vitro multiplication protocol of V. sincorana, analyzing the behavior of in vitro shoots subjected to stress, and its influence on multiplication. They were used as explants in vitro shoots obtained, inoculated with concentrations of BAP (0.0; 2.22; 4.44 ?M), CIN (0.0; 2.32; 4.64 ?M) and IBA (0.0; 1.48 ?M) in MS ? medium supplemented with 87.64 mM sucrose, 1g.L-1activated carbon, and 0.7% agar (basic medium). Subsequently, it tested concentrations of cytokinins BAP or KIN (0.0; 5.0; 10.0; 15.0; 20.0 ?M). In experiments with thermal stress, flame shoots were exposed for different periods (0.0; 2.0; 4.0; 6.0; 8.0; 10 seconds), and the next tested whether the periods (0.0; 4.5; 6.0; 7.5; 9.0 min) exposure of the shoots 105 ? 1 ? C in sterilize. For drought stress basic medium was agar supplemented with different concentrations (7.0; 14.0; 21.0; 28.0 g) and the following experiment different sucrose concentrations (87.64; 175.28; 262. 92; 350.56 mM). The KIN showed greater efficacy in the multiplication in relation to BAP. The highest average for number of shoots and responsive% explants were in the periods 6, 8 or 10 seconds, being observed tolerance to water and thermal stress. / Vellozia sincorana AYENSU & SMITH ? end?mica dos Campos Rupestres da Chapada Diamantina, conhecida popularmente como Candomb?. Essa esp?cie possui resina altamente inflam?vel, sendo o caule utilizado para acender fog?es a lenha e as ra?zes para produzir incenso. O Candomb? apresenta poucos trabalhos na literatura, n?o havendo estudos de propaga??o da esp?cie. Esse trabalho teve como objetivo desenvolver protocolo de multiplica??o in vitro de V. sincorana, analisando o comportamento dos brotos in vitro submetidos ao estresse, e sua influ?ncia na multiplica??o. Foram utilizados como explantes brotos obtidos in vitro, inoculados em concentra??es de BAP (0,0; 2,22; 4,44 ?M), CIN (0,0; 2,32; 4,64 ?M) e AIB (0,0; 1,48 ?M) em meio MS ?suplementado com 87,64mM de sacarose, 1g.L-1 de carv?o ativado, e0,7% de ?gar (meio b?sico).No experimento seguinte, testou-se concentra??es das citocininas BAP ou CIN (0,0; 5,0; 10,0; 15,0; 20,0 ?M). Nos experimentos com estresse t?rmico, os brotos foram expostos a flambagem por diferentes per?odos (0, 2, 4, 6, 8; 10 segundos), e no seguinte testou-se os per?odos (0,0, 4,5, 6,0, 7,5; 9,0 min.) de exposi??o dos brotos a 105?1?C, em estufa. Para estresse h?drico o meio b?sico foi suplementado com diferentes concentra??es de ?gar (7,0, 14,0, 21,0, 28,0 g) e no experimento seguinte diversas concentra??es de sacarose (87,64, 175,28, 262,92; 350,56 mM).O CIN apresentou maior efic?cia na multiplica??o, em rela??o ao BAP. As maiores m?dias para n?mero de brotos e % explantes responsivos foram nos per?odos 6, 8 e 10 segundos, sendo observada toler?ncia aos estresses h?drico e t?rmico.