Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-22T12:08:16Z No. of bitstreams: 2 mauricio_rocha_dourado.pdf: 2196583 bytes, checksum: ffa323001018e93a3c8d5bfd16675742 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-22T12:19:49Z (GMT) No. of bitstreams: 2 mauricio_rocha_dourado.pdf: 2196583 bytes, checksum: ffa323001018e93a3c8d5bfd16675742 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-22T12:20:06Z (GMT) No. of bitstreams: 2 mauricio_rocha_dourado.pdf: 2196583 bytes, checksum: ffa323001018e93a3c8d5bfd16675742 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2014-12-22T12:20:06Z (GMT). No. of bitstreams: 2 mauricio_rocha_dourado.pdf: 2196583 bytes, checksum: ffa323001018e93a3c8d5bfd16675742 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2014 / Amelog?nese imperfeita (AI) representa um complexo grupo de condi??es herdadas que provocam malforma??es do esmalte dent?rio em quantidade ou qualidade. Nefrocalcinose (NC) ? a deposi??o de sais de c?lcio no par?nquima renal que pode, em longo prazo e na aus?ncia de tratamento adequado, levar ? insufici?ncia renal cr?nica e outros dist?rbios renais. Estas duas condi??es, simultaneamente, representam a s?ndrome da amelog?nese imperfeita e nefrocalcinose (SAINC), ou s?ndrome esmalte renal, uma desordem autoss?mica recessiva causada por muta??es no gene FAM20A, com poucos casos relatados na literatura. A proposta do presente estudo foi investigar as caracter?sticas da s?ndrome em indiv?duos de quatro fam?lias brasileiras. Os pacientes foram submetidos ao exame cl?nico, radiogr?fico, investiga??o renal e exames hematol?gicos e de marcadores bioqu?micos, sendo identificados nove pacientes portadores da s?ndrome. O exame cl?nico revelou dentes pequenos e de colora??o amarelada, desgaste oclusal/incisal, reten??o da denti??o dec?dua e hiperplasia gengival. As radiografias mostraram les?es pericoron?rias radiol?cidas envolvendo dentes permanentes impactados, aus?ncia de contraste entre esmalte e dentina e calcifica??es intrapulpares. Os exames bioqu?micos evidenciaram, em quatro pacientes, baixos n?veis de vitamina D 25-OH e altos n?veis de fosfatase alcalina e paratorm?nio. A ultrassonografia revelou nefrocalcinose bilateral, mas, os pacientes ainda, n?o demonstraram nenhuma manifesta??o de doen?a renal. Outras caracter?sticas menos comuns como defici?ncia mental e cistos renais foram encontradas, o que confirma o grande espectro de altera??es fenot?picas desta s?ndrome, j? descrita anteriormente. Estes resultados confirmam as varia??es fenot?picas da SAINC e refor?am a necessidade de investiga??o renal em pacientes com diagn?stico de AI, principalmente do tipo hipopl?sica. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT Amelogenesis imperfecta (AI) is a complex group of conditions that cause inherited defects of dental enamel in quantity or quality. Nephrocalcinosis (NC) is the deposition of calcium salts at the renal parenchyma, which may in the long term and in the absence of proper treatment, lead to chronic renal failure and other kidney disorders. These two conditions simultaneously represent the amelogenesis imperfecta and nephrocalcinosis syndrome (AINCS), or enamel renal syndrome, an autosomal recessive disorder caused by mutations in FAM20A gene, with few cases reported in the literature. The purpose of this study was to investigate the characteristics of the syndrome in patients from four Brazilian families. Patients underwent clinical examination, radiographic, renal and haematological investigation and biochemical markers examinations, being identified nine patients with this syndrome. Clinical examination revealed small and yellowish teeth, occlusal/incisal wear, retention of deciduous dentition and gingival hyperplasia. The radiographs showed pericoronal radiolucencies involving impacted permanent teeth, absence of contrast between enamel and dentin and intrapulpal calcifications. Biochemical tests showed low levels of 25-OH vitamin D and high levels of alkaline phosphatase and parathyroid hormone in four patients. Ultrasonography revealed bilateral nephrocalcinosis, but patients still showed no manifestation of renal disease. Other characteristics as mental retardation and renal cysts were found, confirming the wide spectrum of phenotypic alterations of this syndrome previously described. These results confirm the phenotypic variations AINCS and reinforce the need for kidney research in patients with AI, especially the hypoplastic type.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-11T17:13:31Z No. of bitstreams: 1 PatriciaGuerraPeixeGoncalves_DISSERT.pdf: 943209 bytes, checksum: 858cf53a3d375685e3e69be1d38caba2 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-07-15T20:21:04Z (GMT) No. of bitstreams: 1 PatriciaGuerraPeixeGoncalves_DISSERT.pdf: 943209 bytes, checksum: 858cf53a3d375685e3e69be1d38caba2 (MD5) / Made available in DSpace on 2016-07-15T20:21:04Z (GMT). No. of bitstreams: 1 PatriciaGuerraPeixeGoncalves_DISSERT.pdf: 943209 bytes, checksum: 858cf53a3d375685e3e69be1d38caba2 (MD5) Previous issue date: 2016-02-22 / A angiog?nese e a linfangiog?nese s?o altera??es tamb?m decorrentes da inflama??o gengival provocada por microrganismos presentes no biofilme dental, bem como pela a migra??o de c?lulas de defesa e secre??o de mediadores inflamat?rios no local da agress?o. Este estudo teve por objetivo avaliar a angiog?nese e linfangiog?nese em 90 esp?cimes de bi?psias de tecido gengival clinicamente saud?vel, com gengivite e com periodontite cr?nicas. Os cortes histol?gicos foram avaliados pela colora??o de hematoxilina e eosina e pela t?cnica de imunoistoqu?mica atrav?s da imunomarca??o de CD34 e podoplanina, para avaliar, respectivamente, o ?ndice angiog?nico e linfangiog?nico, por meio da t?cnica de contagem microvascular. Os resultados mostraram que h? correla??o entre os ?ndices (p=0,030), por?m, mostrou que na periodontite h? menos n?meros de vasos linf?ticos do que no tecido gengival clinicamente saud?vel (p=0,016). A podoplanina mostrou marca??o no epit?lio e que h? rela??o da intensidade de marca??o com a intensidade do infiltrado inflamat?rio, sendo mais intensa a marca??o na presen?a de infiltrado inflamat?rio severo (p=0,033). Concluiu-se neste estudo que h? menor n?mero de vasos sangu?neos na periodontite em compara??o com a gengiva clinicamente saud?vel. As sinaliza??es presentes no processo inflamat?rio, bem como o real papel da vasculatura sangu?nea e linf?tica gengival ainda n?o est?o totalmente elucidadas / Angiogenesis and lymphangiogenesis are changes that occur due to gingival inflammation caused by microorganisms present in the biofilm, as well as the migration of immune cells and secretion of mediators in the aggressed site. This study aimed to research angiogenesis and lymphangiogenesis in 90 specimens of clinically healthy, with gingivitis and chronic periodontitis gingival tissue biopsies. The histological sections were evaluated by hematoxylin and eosin and the immunohistochemical technique through immunostaining for CD34 and podoplanin. To evaluate the angiogenic and lymphangiogenic indexes we performed a microvessel counting technique. The results showed that there is a correlation between the indexes (p = 0.030), however, we observed that periodontitis showed less lymphatic vessels than clinically healthy gingival tissue (p = 0.016). Podoplanin showed positive staining in the basal layers of the epithelium, and we observed a relationship between immunostaining intensity and the intensity of inflammatory infiltrate, with more intense staining in the presence of severe inflammatory infiltrate (p = 0.033). For this study, we concluded that there are fewer blood vessels in periodontitis compared with clinically healthy gingiva. The signaling present in the inflammatory process and the actual role of gingival blood and lymphatic vasculature are not fully understood, with further studies on angiogenesis and lymphangiogenesis being suggested.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2017-03-10T15:09:55Z No. of bitstreams: 1 TES_JULIANO_SARTORI_COMPLETO.pdf: 9083435 bytes, checksum: 6cadde5ff4cace9cc886d4131547c873 (MD5) / Made available in DSpace on 2017-03-10T15:09:55Z (GMT). No. of bitstreams: 1 TES_JULIANO_SARTORI_COMPLETO.pdf: 9083435 bytes, checksum: 6cadde5ff4cace9cc886d4131547c873 (MD5) Previous issue date: 2016-12-02 / Despite the advances resulting from the molecular characterization of breast cancer that allowed the use of more selective therapeutic targets, the disease still causes different clinical outcomes with high rates of recurrence and mortality. In this context of complexity and heterogeneity of breast cancer, are important the investigations of new biomolecular markers related to breast oncogenesis that may contribute to know the prognosis and improve the clinical management of patients. The sirtuin-1 (SIRT-1) is a histone deacetylase implicated in various epigenetic critical functions into cells, among these, the maintenance of genomic stability, proliferation and cell aging. The aim of this study was to investigate the grade of expression of the sirtuin- 1 (SIRT-1) in a cohort of 457 women with breast cancer and verify the effect, independent or in combination with other variables in the prognosis of these patients. It is a survival analysis study based on hospital medical records of women with breast cancer undergoing treatment in Erechim-RS from 2003 to 2013 and followed until 31 July 2015. The analysis of the grade of SIRT-1 expression was performed by immunohistochemistry in 123 patients (26.9%) of the total cohort. Overall survival specific disease (OS) was estimated by the Kaplan-Meier method and the risk of death from breast cancer by the method of Cox proportional hazards. The research was approved by the Research Ethics Committee of PUC-RS as reported number 465.362. The median age was 57.4 years and the median estimate of breast cancer survival was 79.6% at 5 years and 69.1% at 10 years, with median follow-up time of 61.9 months. Risk factors associated with worse prognosis were: age between 60 and 69 years (HR = 1.88; 95% CI 1.02 - 3.44; p = 0.042); older than 70 years (HR = 2.92; CI 95% 1.70 - 5.01; p = 0.000); tumor size greater than 2 cm (HR = 1.86; CI 95% 1.04 -3.36; p = 0.038); metastasis in 4 or more axillary lymph nodes (HR = 2.37; CI 95% 1.38 - 4.08; p = 0.002); located clinical staging (CS II, TNM) (HR = 3.39; CI 95% 1.35 - 8.51; p = 0.009); advanced clinical staging (CS III, TNM) (HR = 6.32; CI 95% 2.49-16.08; p = 0.000); high histologic grade (HR = 6.32; CI 95% 1.63 - 29.99; p = 0.008); triple negative molecular profile (HR = 2.33; CI 95% 1.19 - 4.58; p = 0.014) and radical surgery (HR = 2.10; CI 95% 1.31 - 3.36; p = 0.002). The positive expression of progesterone receptor (HR = 0.52, CI 95% 0.34 - 0.79; p = 0.002) was a better prognostic factor for patients. The grade of overexpression of SIRT-1, defined as nuclear expression of SIRT-1 greater than 80% was observed in 6.5% of cases. The SIRT-1 overexpression characterized a subgroup of women who had a worse prognosis with shorter survival and increased risk of death from breast cancer (HR = 2.66; CI 95% 1.03 - 6.86; p = 0.043). Multivariate regressive models (Cox) were constructed and the overexpression of SIRT-1 remained significant statistic demonstrating independent factor associated with worse prognostic in breast cancer. Therefore, the evaluation of the grade of expression of the SIRT-1, in the cohort of Erechim-RS, proved to be an independent prognostic marker for analysis of the risk of death from breast cancer. / Apesar dos avan?os decorrentes da caracteriza??o molecular do c?ncer de mama que permitiram o emprego de alvos terap?uticos mais seletivos, a doen?a ainda ocasiona diferentes desfechos cl?nicos, com elevadas taxas de recidiva e mortalidade. Neste contexto de complexidade e heterogeneidade do c?ncer de mama, s?o importantes as investiga??es de novos marcadores biomoleculares relacionados ? oncog?nese mam?ria que possam contribuir para conhecer o progn?stico e aprimorar o manejo cl?nico das pacientes. A Sirtu?na-1 (SIRT-1) ? uma histona desacetilase implicada em diversas fun??es epigen?ticas cr?ticas para as c?lulas, dentre estas, a manuten??o da estabilidade gen?mica, a prolifera??o e o envelhecimento celular. O objetivo deste estudo foi investigar o grau de express?o da Sirtu?na-1 (SIRT-1) em uma coorte de 457 mulheres portadoras de c?ncer de mama e verificar o seu efeito, independente ou em associa??o a outras vari?veis, no progn?stico destas pacientes. Trata-se de um estudo de an?lise de sobrevida com base em registros de mulheres portadoras de c?ncer de mama submetidas a tratamento em Erechim-RS no per?odo de 2003 a 2013 com seguimento at? 31 de julho de 2015. A an?lise do grau de express?o de SIRT-1 foi realizada por t?cnica de imuno-histoqu?mica em 123 pacientes (26,9%) do total da coorte. A sobrevida global doen?a espec?fica foi estimada pelo m?todo de Kaplan-Meier e, o risco de morte, por c?ncer de mama, pelo m?todo de riscos proporcionais de Cox. A pesquisa foi aprovada no Comit? de ?tica e Pesquisa da PUC-RS, sob n? 465.362. A idade mediana foi de 57,4 anos e a estimativa mediana de sobrevida por c?ncer de mama foi de 79,6% em 5 anos e 69,1% em 10 anos, com tempo mediano de seguimento de 61,9 meses. Os fatores de risco associados a pior progn?stico foram: faixa et?ria entre 60 e 69 anos (HR=1,88; IC95% 1,02-3,44; p=0,042); faixa et?ria maior que 70 anos (HR=2,92; IC95% 1,70-5,01; p=0,000); tamanho tumoral acima de 2 cm (HR=1,86; IC95% 1,04-3,36; p=0,038); presen?a de met?stase em 4 linfonodos axilares ou mais (HR=2,37; IC95% 1,38-4,08; p=0,002); estadiamento cl?nico localizado (EC II,TNM) (HR=3,39; IC95% 1,35-8,51; p=0,009); estadiamento cl?nico avan?ado (EC III,TNM) (HR=6,32; IC95% 2,49-16,08; p=0,000); grau histol?gico alto (HR=6,32; IC95% 1,63-29,99; p=0,008); perfil molecular triplo negativo (HR=2,33; IC95% 1,19-4,58; p=0,014) e a cirurgia radical (HR=2,10; IC95% 1,31-3,36; p=0,002). A express?o positiva do receptor de progesterona (HR=0,52; IC95% 0,34- 0,79; p=0,002) foi um fator de melhor progn?stico para as pacientes. O grau de hiperexpress?o da SIRT-1, definida como express?o nuclear da SIRT-1 maior que 80%, foi verificada em 6,5% dos casos. A hiperexpress?o da SIRT-1 caracterizou um subgrupo de mulheres que apresentaram pior progn?stico, com menor sobrevida e maior risco de morte por c?ncer de mama (HR=2,66; IC95% 1,03-6,86; p=0,043). Foram elaborados modelos regressivos multivariados (Cox) e a hiperexpress?o de SIRT-1 manteve signific?ncia estat?stica demonstrando fator independente associado a pior progn?stico no c?ncer de mama. Portanto, a avalia??o do grau de express?o da SIRT-1, na coorte de Erechim-RS, demonstrou ser um marcador independente para determinar o progn?stico no c?ncer de mama.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-04-11T21:06:17Z No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) / Made available in DSpace on 2017-04-11T21:06:17Z (GMT). No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) Previous issue date: 2016-09-26 / (In vitro propagation and acclimatization of medicinal species: Alpinia zerumbet (Pers.) B.L.Burtt&R.M.Sm. (Zingiberaceae) and Solidago chilensis Meyen (Asteraceae). Alpinia zerumbet and Solidago chilensis are known for their ornamental and medicinal values. There are few reports in the literature on micropropagation of Alpinia zerumbet and none about Solidago chilensis, which demonstrates the need for studies. This work aimed to study in vitro propagation of the species, involving micropropagation and developing protocols for organogenesis. For in vitro establishment were tested different types of explants, disinfestation methods and antioxidants. For multiplication, these explants were tested with plant growth regulators naftalen acetic acid and benzilaminopurin isolated and combined, and dichlorophenoxyacetic acid and kinetin, isolated and combined. It was made anatomical characterization of callus formation. For acclimatization, after pre-acclimatization, the seedlings were transferred to plastic cups containing sterilized substrate, capped with plastic bottles and taken to a greenhouse with 50% shading, where the bottle caps were unscrewed slowly. The results showed success in establishing in vitro of A. zerumbet crucial step to start large-scale cultivation. Against contamination, the most effective treatment was 4ml PPM/L (Plant Preservative Mixture), controlling 100% of pathogens. As an antioxidant, ascorbic acid (2%) was the most efficient. There was budding A. zerumbet derivatives bud explants. There was no decline in the propagation rate during in vitro subcultures, however growth is very slow. S. chilensis, explants nodal segments showed a higher rate of multiplication. There was no decline in the spread rate over four subcultures in vitro and the seedlings had a high survival rate in the acclimatization phase. / Alpinia zerumbet e Solidago chilensis s?o conhecidas pelos valores ornamental e medicinal. Existem poucos relatos na literatura sobre micropropaga??o de Alpinia zerumbet e nenhum sobre Solidago chilensis, o que demonstra necessidade de estudos. Este trabalho objetivou estudar a propaga??o in vitro das esp?cies, envolvendo micropropaga??o e desenvolvendo protocolos para organog?nese. Para o estabelecimento in vitro foram testados diferentes tipos de explantes, m?todos de desinfesta??o e antioxidantes. Para multiplica??o, esses explantes foram testados com reguladores vegetais: ?cido naftalenoac?tico e benzilaminopurina isolados e combinados, al?m de ?cido 2,4-diclorofenoxiac?tico e cinetina, isolados e combinados. Foi feita caracteriza??o anat?mica da calog?nese. Para aclimatiza??o, ap?s a pr?-aclimatiza??o, as mudas foram transferidas para copos pl?sticos contendo substrato esterilizado, tampados com garrafas pet e levados para casa de vegeta??o com sombrite 50%, onde as tampas das garrafas foram desenroscadas aos poucos. Os resultados mostraram sucesso no estabelecimento in vitro de A. zerumbet, etapa crucial para iniciar cultivo em larga escala. Contra contamina??o, o tratamento mais efetivo foi 4mL de PPM/L (Plant Preservative Mixture), controlando 100% dos pat?genos. Como antioxidante, o ?cido asc?rbico (2%) foi o mais eficiente. Houve brota??o de A. zerumbet em explantes derivados de gemas. N?o foi observado decl?nio na taxa de propaga??o no decorrer dos subcultivos in vitro, contudo o crescimento ? muito lento. Para S. chilensis, explantes de segmentos nodais apresentaram maior taxa de multiplica??o. N?o foi observado decl?nio na taxa de propaga??o no decorrer de quatro subcultivos in vitro e as mudas apresentaram alto ?ndice de sobreviv?ncia na fase de aclimatiza??o.

Submitted by Jadson Francisco de Jesus SILVA (jadson@uefs.br) on 2018-01-30T22:51:06Z No. of bitstreams: 1 Tese Definitivo ZERGurgel.pdf: 2392487 bytes, checksum: 97870ae444449d037535f874c18b1d37 (MD5) / Made available in DSpace on 2018-01-30T22:51:06Z (GMT). No. of bitstreams: 1 Tese Definitivo ZERGurgel.pdf: 2392487 bytes, checksum: 97870ae444449d037535f874c18b1d37 (MD5) Previous issue date: 2017-07-24 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Comanthera mucugensis Giul. subsp. mucugensis, an endemic species of the municipality of Mucug?-BA is threatened with extinction. To reduce extractivism in natural populations, it is necessary to develop efficient multiplication protocols; In this sense somatic embryogenesis and organogenesis may be viable and preservation cryopreservation may be a strategy for its long-term. The objectives of this study were: (1) to analyze the embryogenic competence and the indirect organogenesis of C. mucugensis; (2) to evaluate the cryopreservation of seeds and different methods of cryopreservation of C. mucugensis plants and (3) to identify the best way to store seeds in the long term. Experiments were performed to induce somatic embryogenesis with different concentrations of 2,4-D X BAP and Picloran X BAP and for indirect organogenesis BAP and ANA were used. Seeds were cryopreserved for 0 (control), 1, 7, 30, 360 and 540 days and other seeds were stored at different temperatures to verify the best form of storage. Plants were cryopreserved with the technique of vitrification and encapsulation-desiccation. Plants from cryopreserved seeds were acclimatized in sand and vegetal soil (1: 1) for 60 days. The work showed that the plant regulators Picloran and 2,4-D are promising in the induction of callus with embryogenic potential and that the plant regulator ANA at 4.9 ?M is efficient in indirect organogenesis. The seeds of C. mucugensis can be cryopreserved without compromising their physiological quality, but the techniques for cryopreservation of plants have not been efficient. / Comanthera mucugensis Giul. subsp. mucugensis, possui flor de interesse comercial que devido ao extrativismo excessivo encontra-se amea?ada de extin??o. Para suprir a demanda do mercado e evitar o decl?nio populacional, faz-se necess?rio desenvolver protocolos para sua multiplica??o, podendo a embriog?nese som?tica e a organog?nese serem alternativas vi?veis. Al?m disso, ? importante investir em estudos de conserva??o a longo prazo como, por exemplo, a criopreserva??o. O trabalho teve como objetivos: realizar estudos para tornar a micropropaga??o mais eficiente e avaliar a conserva??o de C. mucugensis em diferentes temperaturas como estrat?gia para a conserva??o do seu germoplasma. Foram testados para a indu??o da embriog?nese som?tica 2,4-D x BAP e Picloram x BAP e para organog?nese indireta BAP e ANA. Na criopreserva??o foram avaliadas sementes mantidas em nitrog?nio l?quido (-196?C) por 0, 1, 7, 30, 360 e 540 dias e as plantas inteiras foram submetidas a duas t?cnicas: vitrifica??o e encapsulamento-desidrata??o. Para avaliar o armazenamento, as sementes foram mantidas em temperatura ambiente, 4?C, -20?C, -80?C e -196?C por 30, 90 e 180 dias. Visando observar se a criopreserva??o das sementes interfere no desenvolvimento, plantas oriundas da germina??o in vitro de sementes criopreservadas foram aclimatizadas em areia e terra vegetal (1:1) por 60 dias. O trabalho demonstrou que os reguladores vegetais Picloram e 2,4-D s?o promissores na indu??o de calos com potencial embriog?nico e que o regulador vegetal ANA (4,9 ?M) ? eficiente na organog?nese indireta. As sementes de C. mucugensis podem ser criopreservadas sem comprometer sua qualidade fisiol?gica, entretanto, n?o foram eficientes as t?cnicas para criopreserva??o de plantas inteiras.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-07-19T23:28:56Z No. of bitstreams: 1 DISSERTA??O ANDRESSA PRISCILA PIANC? S. LIMA.pdf: 1903052 bytes, checksum: 3d8a27256e27d81bbdd1cf3ad9eee48f (MD5) / Made available in DSpace on 2016-07-19T23:28:56Z (GMT). No. of bitstreams: 1 DISSERTA??O ANDRESSA PRISCILA PIANC? S. LIMA.pdf: 1903052 bytes, checksum: 3d8a27256e27d81bbdd1cf3ad9eee48f (MD5) Previous issue date: 2016-03-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Chapada Diamantina ? BA presents endemic especies with ornamental potential among which is Orthophytum mucugense, which occurs in the municipality Mucug?. This species is a target of extractivism and it is considered vulnerable, rendering studies of propagation and conservation of the species necessary. Therefore, the objective of this study was to establish protocols of micropropagation and in vitro conservation of O. mucugense. To that end, morphogenetic responses were evaluated in three types of explants, stem, root and leaf under the effect of different concentrations of the plant growth regulators NAA, 2,4-D and BAP. Sprouts formation in stem explants by direct organogenesis, in basal leaf explants by indirect organogenesis and callus formation in basal root and leaf explants were obtained. The rooting of the shoots was carried out with 1 coal g.L-1 activated for 30 days, and microplants acclimatized in a greenhouse with direct exposure to the environment. These results evince that tissue culture is a viable tool for in vitro propagation of O. mucugense. In in vitro conservation the effect of the medium salts reduction, of osmotic agents and of the retardant ancymidol in the minimum growth of the plants were tested; after 300 days of cultivation, analysis of the plant growth, of the chlorophyll content and of the regenerative capacity were carried out. On the basis of these assessments, the ancymidol, in the concentrations used, is not efficient in minimal growth induction, and the reduction of MS salts to ?3, with the combination of 45 g.L-1 of sucrose with 7.8 g.L-1 of mannitol is indicated for in vitro conservation of O. mucugense. / A Chapada Diamantina ? BA apresenta esp?cies end?micas com potencial ornamental dentre as quais est? a Orthophytum mucugense, que ocorre no munic?pio de Mucug?. Esta esp?cie ? alvo de extrativismo e considerada como vulner?vel, sendo necess?rios estudos de propaga??o e conserva??o da mesma. Portanto, objetivou-se neste trabalho estabelecer protocolos de micropropaga??o e conserva??o in vitro de O. mucugense. Para isto foram avaliadas as respostas morfog?nicas em tr?s tipos de explantes, caulinar, radicular e foliar, sob efeito de diferentes concentra??es dos reguladores vegetais ANA, 2,4-D e BAP. Foram obtidas forma??o de brotos em explante caulinar por organog?nese direta, em explante foliar basal por organog?nese indireta e forma??o de calo em explante foliar basal e radicular. O enraizamento dos brotos foi realizado com 1 g.L-1 de carv?o ativado por 30 dias, e as microplantas aclimatizadas em casa de vegeta??o com exposi??o direta ao ambiente. Estes dados demonstram que a cultura de tecidos ? uma ferramenta vi?vel para a propaga??o in vitro de O. mucugense. Na conserva??o in vitro foram testados o efeito da redu??o de sais do meio, de agentes osm?ticos e do retardante ancymidol no crescimento m?nimo das plantas; ap?s 300 dias de cultivo foram realizadas an?lises de crescimento das mesmas, do teor de clorofila e da capacidade regenerativa. Com base nestas avalia??es o ancymidol, nas concentra??es utilizadas, n?o ? eficiente na indu??o do crescimento m?nimo, e a redu??o de sais MS para ?3 com a combina??o de 45 g.L-1 de sacarose com 7,8 g.L-1 de manitol ? indicada para conserva??o in vitro de O. mucugense.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-08-24T20:52:09Z No. of bitstreams: 1 Disserta??o Final_Fl?via_PPGRGV (2).pdf: 1857571 bytes, checksum: 967f2b7d728622a11a98a803f13ff0de (MD5) / Made available in DSpace on 2016-08-24T20:52:09Z (GMT). No. of bitstreams: 1 Disserta??o Final_Fl?via_PPGRGV (2).pdf: 1857571 bytes, checksum: 967f2b7d728622a11a98a803f13ff0de (MD5) Previous issue date: 2015-03-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Hyptis ramosa Pohl ex Benth (Lamiaceae) is native and endemic to the semi-arid northeast, with its unknown phytochemical constitution so far. Considering the pharmacological importance of the species of this family, the development of forms of propagation and in vitro culture can contribute to the inclusion of these species in sustainable production systems and the conservation of the same. Thus, the objective of this work was to study the in vitro propagation of the species H. ramosa, through direct organogenesis and callus formation, and the biochemical characterization of the obtained calluses, thus allowing the establishment of strategies for their conservation and sustainable use. To this, H. ramosa seeds were disinfected and established in medium MS / 2 culture. In the multiplication phase was tested the influence of cytokinins BAP, CIN and TDZ on different explants. The obtained shoots were individualized and transferred to MS / 2 medium containing different concentrations of auxin IBA and activated carbon for rooting them. Regenerated and rooted in vitro microplants were subjected to pre-acclimatization in different cultivation container closure and then were transferred to ex vitro conditions in commercial substrate Plantmax? being quantified plant survival rate at 30 days after transfer. For callus induction, we used explants and different concentrations of 2,4-D and BAP, determining the growth curve from the fresh weight of callus until the 28th day of cultivation, at intervals of seven days. Concurrent with obtaining the growth curve it is quantified in calluses obtained the total soluble sugar content, reducing sugar and crude protein. The in vitro propagation of H. ramosa is possible using the nodal segment as a source of explants in MS medium supplemented with BAP. In vitro rooting occurs, even in free auxin. The species showed survival of 100%, regardless of the day of pre-acclimatization phase. For callus induction the best explant is the nodal segment, and the combination of 2.4-D and BAP favor the formation of the same. The callus growth curve showed quadratic behavior with two different phases and biochemical analysis showed the maximum level of total soluble sugars, reducing sugars and crude protein at 14 ?, 21 ? and 14 ? days, respectively. / Hyptis ramosa Pohl ex Benth (Lamiaceae) ? uma esp?cie nativa e end?mica do semi?rido nordestino, sendo sua constitui??o fitoqu?mica desconhecida at? o momento. Considerando a import?ncia farmacol?gica das esp?cies dessa fam?lia, o desenvolvimento de formas de propaga??o e cultivo in vitro poder? contribuir para a inser??o dessas esp?cies em sistemas de produ??o sustent?veis e a conserva??o das mesmas. Diante disso, o objetivo deste trabalho foi estudar a propaga??o in vitro da esp?cie H. ramosa, atrav?s de organog?nese direta e calog?nese, bem como a caracteriza??o bioqu?mica dos calos obtidos, permitindo assim o estabelecimento de estrat?gias para a sua conserva??o e explora??o sustent?vel. Para isso, sementes de H. ramosa foram desinfestadas e estabelecidas em meio de cultura MS/2. Na fase de multiplica??o foi testada a influ?ncia das citocininas BAP, CIN e TDZ sobre diferentes explantes. As brota??es obtidas foram individualizadas e transferidas para meio MS/2 contendo diferentes concentra??es da auxina AIB e de carv?o ativo para o enraizamento das mesmas. As microplantas regeneradas e enraizadas in vitro foram submetidas ? pr?-aclimatiza??o em diferentes tipos de fechamento do recipiente de cultivo e, posteriormente, foram transferidas para a condi??o ex vitro em substrato comercial Plantmax?, sendo quantificada a taxa de sobreviv?ncia das plantas aos 30 dias ap?s a transfer?ncia. Para a indu??o de calos utilizou-se diferentes explantes e concentra??es de 2,4-D e BAP, determinando-se a curva de crescimento a partir da mat?ria fresca dos calos at? o 28o dia de cultivo, em intervalos de sete dias. Concomitante com a obten??o da curva de crescimento quantificou-se nos calos obtidos o teor de a??cares sol?veis totais, a??cares redutores e prote?na bruta. A propaga??o in vitro de H. ramosa ? poss?vel utilizando-se o segmento nodal como fonte de explante, em meio de cultura MS suplementado com BAP. O enraizamento in vitro ocorre, mesmo em meio isento de auxina. A esp?cie apresentou sobreviv?ncia de 100%, independentemente da realiza??o da fase de pr?-aclimatiza??o. Para indu??o de calos o melhor explante ? o segmento nodal, sendo a combina??o de 2.4-D e BAP favor?vel a forma??o dos mesmos. A curva de crescimento de calos mostrou comportamento quadr?tico com duas fases distintas e a an?lise bioqu?mica evidenciou o teor m?ximo de a??cares sol?veis totais, a??cares redutores e prote?na bruta aos 14?, 21? e 14? dias, respectivamente.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-07-03T11:57:24Z No. of bitstreams: 1 TES_CHARLES_IRAPUAN_FERREIRA_BORGES_COMPLETO.pdf: 1320542 bytes, checksum: 3f221b8b6ff490b017531b0cab345218 (MD5) / Made available in DSpace on 2017-07-03T11:57:24Z (GMT). No. of bitstreams: 1 TES_CHARLES_IRAPUAN_FERREIRA_BORGES_COMPLETO.pdf: 1320542 bytes, checksum: 3f221b8b6ff490b017531b0cab345218 (MD5) Previous issue date: 2017-03-28 / This study presents the thesis I have been calling ?epigenesis of consciousness?. The main problem addressed here is how to account for a strictly materialist explanation of consciousness without resorting to an unrestricted attachment to any sort of reductive materialism and determinist naturalism. My working hypothesis takes as its starting point the Spinozist monism to state: 1. that consciousness emerges from the self-organizing matter; 2. that this self-organization follows a pattern, a layered formation which runs from the peripheral nervous system to the neocortex; 3. that this process is related to the neuroplasticity of the brain tissue and can be mapped by dynamic systems theory. Starting from this hypothesis, I apply the neurodynamics of emotions to map the three stages behind the uprising of consciousness: 1. the live present; 2. Memory; 3. Thought. The aim of this work is to state that consciousness is an evolutionary, co-emergent, subject-to-variation process, merging determinism and freedom in nature. / O presente trabalho tem a pretens?o de defender a tese que venho chamando de epig?nese da consci?ncia. O principal problema abordado aqui ? como apresentar uma leitura estritamente materialista das condi??es de emerg?ncia consci?ncia sem apelar para uma vincula??o irrestrita ao reducionismo e ao determinismo. Minha hip?tese de trabalho toma como ponto de partida o monismo espinosista para afirmar: 1. que a consci?ncia emerge da auto-organiza??o material; 2. que esta auto-organiza??o obedece um padr?o, uma forma??o em camadas, que vai do sistema nervoso perif?rico ao neoc?rtex cerebral; 3. que essa forma??o envolve a neuroplasticidade e pode ser mapeada pela teoria dos sistemas din?micos. Partindo dessa hip?tese, aplico os conceitos da neurodin?mica das emo??es para mapear as tr?s etapas da forma??o da consci?ncia, as tr?s s?nteses passivas: 1. presente vivo; 2. mem?ria; 3. pensamento. O objetivo do presente trabalho ? fundamentar a tese segundo a qual a forma??o da consci?ncia ? um processo evolutivo, coemergente e sujeito ? varia??o. Com isso, pretendo sustentar uma vis?o de ag?ncia que prop?e que entendamos como n?o mutuamente excludentes determinismo e liberdade, na natureza.

Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2018-03-26T16:55:48Z No. of bitstreams: 1 RODRIGO_BRACCINI_MADEIRA_DA_SILVA_TES.pdf: 6388000 bytes, checksum: c5e864eb62bd553c3a29e7bad1b85fef (MD5) / Rejected by Tatiana Lopes (tatiana.lopes@pucrs.br), reason: Devolvido porque o pdf inserido no TEDE ? de outro aluno. on 2018-03-28T14:20:43Z (GMT) / Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2018-03-28T14:24:15Z No. of bitstreams: 1 FERNANDA_MAJOLO_TES.pdf: 6002507 bytes, checksum: 764acb66fc06c518428a40b9eb5bdd26 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-28T16:23:42Z (GMT) No. of bitstreams: 1 FERNANDA_MAJOLO_TES.pdf: 6002507 bytes, checksum: 764acb66fc06c518428a40b9eb5bdd26 (MD5) / Made available in DSpace on 2018-03-28T16:29:23Z (GMT). No. of bitstreams: 1 FERNANDA_MAJOLO_TES.pdf: 6002507 bytes, checksum: 764acb66fc06c518428a40b9eb5bdd26 (MD5) Previous issue date: 2018-01-11 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Malformations of cortical development (MDC) include a wide spectrum of Central Nervous System (CNS) disorders related to a complex process of cortex formation. Focal Cortical Dysplasia (FCD), a common type of MDC, is reported as the most frequent structural brain lesion found in children with refractory epilepsy to drug treatment undergoing surgery. Surgical treatment, with complete resection of the dysplastic lesion, is able to stop the seizure resistant to antiepileptic drugs, improving the individual's quality of life and reducing morbidity. FCD is characterized by multiple types of alterations both in cortical architecture and in cytologic abnormalities and it?s pathogenesis is still unknown. In 2004, Palmini et al. classified DCF according to white matter and cortical layer architecture. Taylor-type FCD is characterized by cortical laminar disorganization and dysplastic neurons, compromising the organization of the cortex into six-layered traditionally known. Understanding the mechanisms of action of neurological diseases has involved the use of animal models. However, in the case of brain development of epileptic syndromes, many decades of study have failed to provide a conclusive insight of their mechanisms. Modeling neurological diseases is especially attractive for the application of induced pluripotent stem cells (iPSCs), making possible to derive specific neurons for in vitro studies, contributing to the investigation of the disease. The aim of the present study was to investigate the possible differences in neurogenesis and neurodifferentiation of iPSCs from fibroblasts of individuals affected by Taylor-type FCD and normal individuals. iPSCs were generated from skin fibroblasts of two FCD individuals and two healthy individuals, to form the control group. The reprogramming was done through the fibroblasts exposure to viral vectors containing the OCT4, KLF4, SOX2, and c-MYC genes and the clones were characterized by immunohistochemistry. iPSCs were neurodifferentiated and analyzed at the 14th, 22nd and 35th days. We also analyzed the brain tissue, fibroblasts and iPSCs cells from the individuals. Through qRT-PCR, the expression of 14 genes involved in the neurodifferentiation process were quantified. These genes are associated to neural migration and differentiation, synaptic aspects and Notch signaling. Both individuals were diagnosed with Taylor-type FCD, more specifically, type IIb. In general, individuals with dysplasia presented alterations in the relative quantification in the most genes analyzed compared to control individuals in all processes and study groups (fibroblasts, brain tissue, iPSCs 8 and neurodifferentiated cells). The genes involved in the neural migration and differentiation processes, as well as synaptic aspects and Notch signaling presented quite altered expressions in dysplastic individuals, with the beginning of the majority processes early, before the physiologically typical period. From the found results, we can infer that during the embryonic period, in the neurogenesis and neurodifferentiation process, individuals affected by the disease, possibly presents neuroblasts more sensitive to stimulus, presenting differences in the development of the Nervous System. These changes may be directly related to dysplastic brain development. This work extends the understanding of embryonic neurodevelopment, open up opportunities to further investigations of the involvement and influence of each genes analyzed in the pathogenesis of FCD, as well as in each mechanism of action involved in the brain development. / As Malforma??es do Desenvolvimento Cortical (MDC) re?nem uma ampla gama de patologias do Sistema Nervoso Central (SNC) relacionadas a um complexo processo de forma??o do c?rtex. A Displasia Cortical Focal (DCF), tipo comum de MDC, ? relatada como a les?o cerebral estrutural mais frequente encontrada em crian?as com epilepsia refrat?ria ao tratamento medicamentoso submetidas ? cirurgia. O tratamento cir?rgico, com a ressec??o completa da les?o displ?sica, ? capaz de cessar a convuls?o resistente a drogas antiepil?ticas, melhorando a qualidade de vida do indiv?duo e diminuindo a morbidade. A DCF ? caracterizada por m?ltiplos tipos de altera??es tanto na arquitetura cortical quanto em anormalidades citol?gicas e sua patog?nese ainda ? desconhecida. Em 2004, Palmini e colaboradores classificaram as DCF de acordo com observa??es na subst?ncia branca e na arquitetura da camada cortical. A DCF do tipo Taylor ? caracterizada por uma desorganiza??o laminar e neur?nios displ?sicos, comprometendo a organiza??o do c?rtex em seis camadas histol?gicas tradicionalmente conhecidas. A compreens?o dos mecanismos de a??o das doen?as neurol?gicas tem envolvido o uso de modelos animais. Por?m, no caso do desenvolvimento cerebral das s?ndromes epil?pticas muitas d?cadas de estudo n?o conseguiram fornecer uma vis?o conclusiva sobre seus mecanismos. Modelar doen?as neurol?gicas ? especialmente atraente para aplica??o das c?lulas pluripotentes induzidas (iPSCs), possibilitando derivar neur?nios espec?ficos do pr?prio paciente para estudos in vitro, contribuindo para a investiga??o da doen?a. O objetivo do presente estudo foi investigar as poss?veis diferen?as na neurog?nese e neurodiferencia??o de iPSCs a partir de fibroblastos de indiv?duos acometidos pela DCF do tipo Taylor e indiv?duos normais. As iPSCs foram geradas a partir de fibroblastos de pele de dois indiv?duos displ?sicos e dois indiv?duos saud?veis, para compor o grupo controle. A reprograma??o se deu atrav?s da exposi??o dos fibroblastos a vetores virais contendo os genes OCT4, KLF4, SOX2, e c-MYC e os clones gerados foram caracterizados por imunohistoqu?mica. As c?lulas iPSCs foram neurodiferenciadas e analisadas nos per?odos de 14, 22 e 35 dias. Tamb?m foram analisados o tecido cerebral, fibroblastos e c?lulas iPSCs dos indiv?duos. Atrav?s de qRT-PCR, a express?o de 14 genes envolvidos no processo de neurodiferencia??o foram quantificados. Estes genes est?o associados a migra??o e diferencia??o neural, 6 aspectos sin?pticos e sinaliza??o Notch. Ambos os indiv?duos foram diagnosticados com DCF do tipo Taylor, mais especificadamente, do tipo IIb. No geral, os indiv?duos displ?sicos apresentaram altera??es na quantifica??o relativa na maioria dos genes analisados comparados aos indiv?duos controle, em todos os processos e grupos de estudo (fibroblastos, tecido cerebral, iPSCs e c?lulas neurodiferenciadas). Os genes envolvidos nos processos de migra??o e diferencia??o neural, aspectos sin?pticos e sinaliza??o Notch apresentaram express?es bastante alteradas nos indiv?duos displ?sicos, com o in?cio da maioria dos processos precoces, antes do per?odo fisiologicamente t?pico. A partir dos resultados encontrados, podemos inferir que durante o per?odo embrion?rio, no processo de neurog?nese e neurodiferencia??o, indiv?duos acometidos pela doen?a, possivelmente possuem neuroblastos mais sens?veis a est?mulos, apresentando diferen?as no desenvolvimento do Sistema Nervoso. Essas altera??es podem estar diretamente relacionadas com a forma??o do c?rebro displ?sico. Este trabalho amplia a compreens?o do neurodesenvolvimento embrion?rio, abrindo portas para futuras investiga??es de forma mais aprofundada sobre o envolvimento e influ?ncia de cada um dos genes analisados na patog?nese da DCF, bem como em cada mecanismo de a??o envolvido na forma??o do c?rebro.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-10-30T20:40:03Z No. of bitstreams: 1 NATALINA - DISSERTA??O.pdf: 1817791 bytes, checksum: 2acedf4e19b6b8a7b03cc91b0abc9111 (MD5) / Made available in DSpace on 2017-10-30T20:40:03Z (GMT). No. of bitstreams: 1 NATALINA - DISSERTA??O.pdf: 1817791 bytes, checksum: 2acedf4e19b6b8a7b03cc91b0abc9111 (MD5) Previous issue date: 2016-07-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The tomatillo center of origin is in Mexico, being the fifth most produced vegetable in the country mainly used in the preparation of traditional Mexican cultural dishes. The principal means of spreading species from the genus Physalis are seeds. Physalis is considered easy to grow because of the quantity of seeds the genus produces, and its high rates of germination. This study aimed to characterize the developmental cycle and compare the growth of tomatillo 'purple' in two light conditions. For this purpose, P. ixocarpa 'purple' was grown in PVC pots with four replications of 10 samples for each treatment, a total of 40 plants per treatment. Evaluation of the seedling emergence was held in a range of 7, 14 and 21 days after sowing. The analysis of other phenophases started up to 26 days after sowing, was maintained throughout the cycle, and held in 14 days intervals. The estimated physiological parameters are the content of chlorophyll a and b, and leaf carotenoids. The plasticity of the species in the study was assessed using growth analysis with the variables height, stem diameter, number of leaves, buds, flowers and fruits, and dry weight of plant growth. Analysis was performed with the variables height, stem diameter, number of leaves, buds, flowers, fruits, dry mass of plants and leaf anatomy. The means of the data were assessed using analysis of variance (ANOVA) and the student T-Test with a 5% significance level. In this study the parameters that were influenced by light were the emergence rate of seedlings, the content of photosynthetic pigments, leaf thickness, period required for the establishment of phenophases, dry weight, fruit production and seed vigor. Although the plants grown in direct sunlight have higher rates of growth, greater production of fruits and viable seeds were obtained in plants grown under shade. / O centro de origem de Physalis ixocarpa ? o M?xico, sendo a quinta hort?cola mais produzida no pa?s, usada principalmente na prepara??o de pratos tradicionais da cultura mexicana. A principal forma de propaga??o de esp?cies do g?nero Physalis ? a partir de sementes, sendo considerada de f?cil cultivo pela quantidade de sementes produzidas e pelas altas taxas de germinabilidade. O presente trabalho teve como objetivo caracterizar o ciclo de desenvolvimento e comparar o crescimento de Physalis ixocarpa Brot. ex Hormen bem como analisar os pigmentos cloroplast?dicos, anatomia foliar e germina??o de sementes oriundos dos frutos produzidos sob duas condi??es de luminosidade. Para tanto, realizou-se o cultivo de P. ixocarpa ?roxa? em vasos de PVC, sendo 4 repeti??es de 10 amostras para cada tratamento, perfazendo um total de 40 plantas por tratamento. As avalia??es de emerg?ncia de pl?ntulas foram realizadas em num intervalo de 7 dias ap?s a semeadura finalizando aos 21, as an?lises das demais fenofases iniciaram-se aos 26 dias ap?s a semeadura sendo mantida por todo ciclo e realizada em intervalo14 dias. O par?metro fisiol?gico avaliado foi o teor de clorofila a e b e caroten?ide de folhas expandidas. A aclimata??o da esp?cie em estudo foi avaliada atrav?s da an?lise de crescimento com as vari?veis: altura, di?metro do colo, n?mero de folhas, bot?es florais, flores e frutos e massa seca das plantas. Foi realizada an?lise de crescimento com as vari?veis: altura, di?metro do colo, n?mero de folhas, bot?es florais, flores, frutos, massa seca das plantas e a anatomia foliar. Os dados foram submetidos ao teste de m?dias com an?lise de vari?ncia seguida pelo Teste T-Student a 5% de signific?ncia. Nesse estudo os par?metros que mostraram-se influenciados pela luminosidade foram a taxa de emerg?ncia de pl?ntulas, os teores de pigmentos fotossint?ticos, espessura do limbo foliar, per?odo necess?rio para o estabelecimento das fenofases, massa seca, produ??o de frutos e vigor das sementes. Apesar das plantas cultivadas a pleno sol apresentarem maiores ?ndices de crescimento, a maior produ??o de frutos e sementes vi?veis foram obtidas em plantas cultivadas em ambiente sombreado.