No description available.

Yes / The present study explored associations between food choice motives, attitudes towards and intention to adopt personalised nutrition, to inform communication strategies based on consumer priorities and concerns. Design/Setting: A survey was administered online which included the Food Choice Questionnaire (FCQ) and items assessing attitudes towards and intention to adopt personalised nutrition. Subjects: Nationally representative samples were recruited in nine EU countries (n 9381). Results: Structural equation modelling indicated that the food choice motives ‘weight control’, ‘mood’, ‘health’ and ‘ethical concern’ had a positive association and ‘price’ had a negative association with attitude towards, and intention to adopt, personalised nutrition. ‘Health’ was positively associated and ‘familiarity’ negatively associated with attitude towards personalised nutrition. The effects of ‘weight control’, ‘ethical concern’, ‘mood’ and ‘price’ on intention to adopt personalised nutrition were partially mediated by attitude. The effects of ‘health’ and ‘familiarity’ were fully mediated by attitude. ‘Sensory appeal’ was negatively and directly associated with intention to adopt personalised nutrition. Conclusions: Personalised nutrition providers may benefit from taking into consideration the importance of underlying determinants of food choice in potential users, particularly weight control, mood and price, when promoting services and in tailoring communications that are motivationally relevant.

Yes / The notion of educating the public through generic healthy eating messages has pervaded dietary health promotion efforts over the years and continues to do so through various media, despite little evidence for any enduring impact upon eating behaviour. There is growing evidence, however, that tailored interventions such as those that could be delivered online can be effective in bringing about healthy dietary behaviour change. The present paper brings together evidence from qualitative and quantitative studies that have considered the public perspective of genomics, nutrigenomics and personalised nutrition, including those conducted as part of the EU-funded Food4Me project. Such studies have consistently indicated that although the public hold positive views about nutrigenomics and personalised nutrition, they have reservations about the service providers’ ability to ensure the secure handling of health data. Technological innovation has driven the concept of personalised nutrition forward and now a further technological leap is required to ensure the privacy of online service delivery systems and to protect data gathered in the process of designing personalised nutrition therapies.

yes / Objective: This study explored associations between food choice motives, attitudes towards, 5 and intention to adopt personalised nutrition in order to inform communication strategies 6 based on consumer priorities and concerns. Design and Setting: A survey was administered 7 online which included the food choice questionnaire (FCQ), and items assessing attitudes 8 towards and intention to adopt personalised nutrition. Participants: Nationally representative 9 samples were recruited in 9 EU countries (N=9381). Results: Structural equation modelling 10 indicated that the food choice motives, weight control, mood, health and ethical concern had 11 a positive association and price had a negative association with attitude towards, and 12 intention to adopt, personalised nutrition. Health was positively associated and familiarity 13 negatively associated with attitude toward personalised nutrition. The effects of weight 14 control, ethical concern, mood and price on intention to adopt personalised nutrition were 15 partially mediated by the attitude. The effects of health and familiarity were fully mediated 16 by attitude. Sensory appeal was negatively and directly associated with intention to adopt 17 personalised nutrition. Conclusion: Personalised nutrition providers may benefit from taking 18 into consideration the importance of underlying determinants of food choice, particularly 19 weight control, mood and price, in potential users when promoting services and in tailoring 20 communications that are motivationally relevant. / Post peer-review accepted manuscript; changes are in red.

Nutrigenômica representa disciplina cientifica recente que surgiu na era do pós-genoma humano, sendo seu foco de estudo a interação gene - nutriente. Profissionais com educação universitária que atuam na área da alimentação e nutrição e da saúde relatam ter dificuldades no entendimento dos princípios dessa disciplina. Recursos digitais como animações representam ferramentas interessantes para o processo de ensino-aprendizagem da nutrigenômica. Dessa forma o objetivo do presente trabalho foi o de desenvolver material educativo digital intitulado \"Fundamentos da Nutrigenômica\", baseado nas animações \"Modulação da expressão gênica por nutrientes e compostos bioativos dos alimentos\" e \"Nutrigenômica e redução do risco de câncer\", para divulgação científica da nutrigenômica para profissionais da área da alimentação e nutrição e da saúde. Para desenvolver o material educativo digital, utilizou-se esquema que se divide nas seguintes etapas: análise e planejamento; modelagem; implementação; avaliação e manutenção. As animações foram produzidas nos programas PowerPoint 2007 e E.M PowerPoint Vídeo Converter. Avaliou-se o material educativo digital por meio de questionário junto a 20 profissionais (nutricionistas, farmacêuticos, engenheiros de alimentos, biólogos, químico, dentista e zootecnista) matriculados ou não em Programas de Pós-Graduação da Universidade de São Paulo nas áreas da alimentação e nutrição e da saúde. Mais de 80% dos participantes concordaram fortemente ou concordaram com as seguintes afirmações: \"O material educativo digital é de fácil manuseio\"; \"A utilização desse tipo de recurso tem vantagens sobre textos\"; \"O material educativo digital trouxe conhecimentos novos sobre Nutrigenômica\"; \"Os conceitos estão expostos de forma clara\"; \"Meus conhecimentos anteriores foram suficientes para aprender com o material educativo digital\"; \"Os textos estão redigidos com clareza\"; \"As animações auxiliam a compreender os fenômenos exibidos\"; \"Aprendi bastante sobre os fundamentos da nutrigenômica com o material educativo digital\". Conclui-se que o material educativo digital \"Fundamentos da nutrigenômica\" representa ferramenta útil para divulgação científica da nutrigenômica junto a profissionais da área da alimentação e nutrição e da saúde. / Nutrigenomics represents recent scientific discipline that has emerged in the post-human genome era, with focus on gene - nutrient interaction. Professionals with university education working in the field of food and nutrition and health report having difficulties in understanding the principles of this discipline. Digital resources such as animations represent interesting tools for the teaching and learning of nutrigenomics. Thus the objective of this study was to develop digital learning material entitled \"Fundamentals of Nutrigenomics\", based on the animations \"Modulation of gene expression by nutrients and bioactive food compounds\" and \"Nutrigenomics and risk reduction of cancer,\" for scientific dissemination of nutrigenomics for professionals in the food and nutrition and health areas. To develop the digital learning material, we used the scheme that is comprised by the following steps: analysis and planning, modeling, implementation, evaluation and maintenance. The animations were produced with the PowerPoint 2007 and EM Video Converter softwares. We evaluated the digital educational materials through a questionnaire provided to 20 professionals (nutritionists, pharmacists, food engineers, biologists, chemist, dentist and zootechnist) registered or not in Graduate Programs from University of Sao Paulo in the areas of food and nutrition and health. Over 80% of participants strongly agreed or agreed with the following statements: \"The digital educational material is easy to handle\", \"The use of this resource type presents advantages over text\", \"The digital learning material brought new knowledge on Nutrigenomics\" , \"The concepts are clearly exposed\", \"My previous knowledge was sufficient to learn from the digital learning material\", \"The texts are written with clarity\", \"The animations help to understand the exhibited phenomena\", \"I learned a lot about nutrigenomics fundamentals with the digital learning materials\". We conclude that the digital learning material \"Fundamentals of Nutrigenomics\" represents a useful tool for scientific dissemination of nutrigenomics for professionals in the field of food and nutrition and health.

Estudos têm mostrado que a atividade da enzima glutationa peroxidase (GPx) se encontra reduzida na presença do polimorfismo de nucleotideo único (SNP) Pro198Leu no gene que codifica para a GPx1. Associado a isso, polimorfismos na região promotora do gene do fator de transcrição Nrf2, o qual se liga ao elemento de resposta antioxidante na via de expressão de genes de enzimas antioxidantes, também pode alterar a expressão gênica da GPx1. Como o mineral selênio faz parte do sítio catalítico desta enzima antioxidante, muitos estudos têm associado o estado nutricional relativo a este nutriente com doenças relacionadas ao estresse oxidativo, como o diabetes mellitus tipo 1 (DM1). Nesse sentido, este estudo visou avaliar a presença dos SNPs Pro198Leu e -617 C/A no Nrf2, bem como da expressão gênica da GPx1 em pacientes com diabetes mellitus tipo 1 e relacioná-los com marcadores do estado nutricional relativo ao selênio e de estresse oxidativo, comparados com um grupo controle sem a doença. Este é um estudo caso e controle, constituído por dois grupos experimentais, um grupo composto por 77 pacientes com diabetes mellitus tipo 1 (DM1) atendidos no Setor de Endocrinologia do Hospital das Clínicas, com idade entre 10 e 19 anos, de ambos os gêneros, e um grupo controle (GC) constituído por 74 indivíduos da mesma faixa etária, os quais relataram ausência de doenças crônicas. Foi realizada avaliação antropométrica e da ingestão alimentar. Além disso, foram determinados parâmetros bioquímicos de status de selênio, controle glicêmico (glicose sérica, HbA1c), atividade enzimática, concentração de malondialdeído e 8-isoprostanos. A determinação dos SNPs e da expressão gênica foi realizada por PCR em tempo real. O grupo DM1 apresentou média de idade de 15,9 anos e o GC de 13,4 anos. A concentração de glicose sérica e HbA1c foi significativamente diferente entre os grupos (p<0,001). As frequências dos genótipos do SNP da GPx1 para o grupo DM1 e GC foram, respectivamente, 60% e 61% (CC), 30% e 32% (CT), 10% e 7% (TT),estando em equilíbrio gênico. A concentração de selênio no plasma foi significativamente maior no grupo DM1 e, ao avaliar essa concentração de acordo com genótipos, observou-se menor concentração de selênio no plasma no genótipo TT no grupo controle (p<0,05). A expressão gênica da GPx1 não apresentou diferença estatística entre os grupos nem entre os genótipos. O mesmo foi observado quanto à concentração de 8-isoprostanos. No entanto, a atividade das enzimas GPx e SOD assim como a concentração de MDA foram significantemente maiores no grupo DM1 (p<0,05). O estado nutricional de todos participantes em relação ao selênio estava deficiente. Foi observada correlação entre a concentração de selênio no plasma e nos eritrócitos e a atividade da GPx, assim como foi observada maior atividade enzimática e concentração de MDA no grupo DM, sem apresentar diferença na distribuição segundo os alelos estudados. Desta forma, pode-se concluir que independentemente da doença todos os indivíduos apresentaram deficiência de selênio. Em ambos os grupos, foi observada maior peroxidação lipídica, na presença do alelo variante T, o que pode indicar alteração da proteção antioxidante. No entanto, a presença do alelo variante nos SNPs avaliados não apresentaram influencia sobre a expressão gênica. / Studies have shown that the activity of glutathione peroxidase (GPx) is reduced in the presence of single nucleotide polymorphism (SNP) in Pro198Leu encoding GPx1 gene. Beyond that, polymorphism of the transcription factor Nrf2 gene promoter, which binds to the antioxidant response element in the pathway of antioxidant enzymes gene expression, may also alter gene expression of GPx1. As mineral selenium is part of the catalytic site of this antioxidant enzyme, many studies have associated the nutritional status of this nutrient with oxidative stress diseases, such as type 1 diabetes mellitus (DM1). Thus, this study aimed to evaluate the presence of Pro198Leu and -617 C / A in Nrf2 SNPs, as well as GPx1 gene expression in patients with type 1 diabetes mellitus, and to associate them with nutritional status of selenium and stress oxidative markers, comparing with a control group without the disease. This is a case-control study, compound of two groups, one group containing 77 patients with type 1 diabetes mellitus (DM1) from the Service of Endocrinology of Hospital das Clinicas, aged between 10 and 19 years, of both genders, and a control group (CG) was composed for 74 individuals of the same age, who reported no chronic diseases. Anthropometric and dietary intake assessment was performed. In addition, the biochemical parameters of selenium status, glycemic control (serum glucose, HbA1c), enzyme activity, concentration of malondialdehyde and 8-isoprostane were determined. The determination of SNPs and gene expression was performed by real-time PCR. The DM1 group had a mean age of 15.9 years and 13.4 years for the CG. The concentration of serum glucose and HbA 1c were significantly different between groups (p <0.001). The genotype frequencies of the GPx1 SNP for DM1 and control group were, respectively, 60% and 61% (CC), 30% and 32% (CT), 10% and 7% (TT), which is in genetic equilibrium. The selenium concentration in plasma was significantly higher in DM1 group. To assess the selenium concentration according to genotypes, we observed lower plasma concentration in TT genotype in the control group (p <0.05). The GPx1 gene expression showed no statistical difference between groups or between genotypes. The same result was observed for the 8-isoprostane concentration. However, GPx and SOD activity and MDA concentration were significantly higher in DM1 (p <0.05). The nutritional status of all participants in relation to selenium was deficient. Correlation between selenium concentration in plasma and erythrocytes and GPx activity, as well as higher enzyme activity and MDA concentration in the DM1 group were observed, with no significant difference in the distribution according to studied alleles was observed. The presence of the variant allele in the SNPs evaluated showed influence neither on gene expression, nor on the activity of GPx. Thus, it can be concluded that, regardless of the disease, all subjects had low nutritional status of selenium, without genotype influence, and, as expected, oxidative stress was increased in individuals with DM1, as demonstrated by laboratory tests.

Nutrigenomics studies the genome-wide influence of nutrients to understand the association between nutrition and human health. Studies in animal models and humans have demonstrated that dietary n-3 polyunsaturated fatty acids (PUFA) from fish oil may be beneficial in inflammatory bowel diseases (IBD). This thesis aimed to test the hypothesis that dietary n-3 PUFA eicosapentaenoic acid (EPA) reduced and n-6 PUFA arachidonic acid (AA) increased colitis in the interleukin- 10 gene-deficient (Il10–/–) mouse model of IBD, and that these PUFA altered the intestinal bacteria community during colitis development using genome-wide expression and bacterial profiling. Using a combined transcriptomic and proteomic approach, the time-course study defined the onset and progression of colitis in Il10–/– mice. Histopathology, transcript and protein changes before and after colitis onset involved in innate and adaptive immune responses suggested delayed remodelling processes in colitic Il10–/– mice and 11 weeks of age as suitable time point to study the effects of dietary PUFA on colitis development. Comparing the transcriptome and proteome profiles associated with colon inflammation of mice fed with the AIN-76A or oleic acid (OA) diet showed that OA was an appropriate control for unsaturated fatty acids in multi-omic studies. The PUFA intervention study indicated that dietary EPA-induced lipid oxidation might have a potential anti-inflammatory effect on inflamed colon tissue partially mediated through activation of peroxisome proliferator-activated receptor alpha (PPARα). Unexpectedly, dietary AA decreased the expression of inflammatory and stress colonic genes in Il10–/– mice. Altered intestinal bacteria community observed in Il10–/– mice before and after colitis onset was associated with the lack of IL10 protein led to changes in intestinal metabolic and signalling processes. Interestingly, dietary EPA and AA seemed to change intestinal bacteria profiles during colitis development. The role of PPARα in the colon was further examined in a concluding study which identified vanin1 as a likely new PPARα-target gene which may also be involved in lipid metabolism. These findings using a state-of-the-art approach combining transcriptomics, proteomics and physiology provide a basis for future research on molecular mechanisms underlying the effects of dietary PUFA, and might contribute to the development of fortified foods that improve intestinal health and wellness.

Nutrigenômica representa disciplina cientifica recente que surgiu na era do pós-genoma humano, sendo seu foco de estudo a interação gene - nutriente. Profissionais com educação universitária que atuam na área da alimentação e nutrição e da saúde relatam ter dificuldades no entendimento dos princípios dessa disciplina. Recursos digitais como animações representam ferramentas interessantes para o processo de ensino-aprendizagem da nutrigenômica. Dessa forma o objetivo do presente trabalho foi o de desenvolver material educativo digital intitulado \"Fundamentos da Nutrigenômica\", baseado nas animações \"Modulação da expressão gênica por nutrientes e compostos bioativos dos alimentos\" e \"Nutrigenômica e redução do risco de câncer\", para divulgação científica da nutrigenômica para profissionais da área da alimentação e nutrição e da saúde. Para desenvolver o material educativo digital, utilizou-se esquema que se divide nas seguintes etapas: análise e planejamento; modelagem; implementação; avaliação e manutenção. As animações foram produzidas nos programas PowerPoint 2007 e E.M PowerPoint Vídeo Converter. Avaliou-se o material educativo digital por meio de questionário junto a 20 profissionais (nutricionistas, farmacêuticos, engenheiros de alimentos, biólogos, químico, dentista e zootecnista) matriculados ou não em Programas de Pós-Graduação da Universidade de São Paulo nas áreas da alimentação e nutrição e da saúde. Mais de 80% dos participantes concordaram fortemente ou concordaram com as seguintes afirmações: \"O material educativo digital é de fácil manuseio\"; \"A utilização desse tipo de recurso tem vantagens sobre textos\"; \"O material educativo digital trouxe conhecimentos novos sobre Nutrigenômica\"; \"Os conceitos estão expostos de forma clara\"; \"Meus conhecimentos anteriores foram suficientes para aprender com o material educativo digital\"; \"Os textos estão redigidos com clareza\"; \"As animações auxiliam a compreender os fenômenos exibidos\"; \"Aprendi bastante sobre os fundamentos da nutrigenômica com o material educativo digital\". Conclui-se que o material educativo digital \"Fundamentos da nutrigenômica\" representa ferramenta útil para divulgação científica da nutrigenômica junto a profissionais da área da alimentação e nutrição e da saúde. / Nutrigenomics represents recent scientific discipline that has emerged in the post-human genome era, with focus on gene - nutrient interaction. Professionals with university education working in the field of food and nutrition and health report having difficulties in understanding the principles of this discipline. Digital resources such as animations represent interesting tools for the teaching and learning of nutrigenomics. Thus the objective of this study was to develop digital learning material entitled \"Fundamentals of Nutrigenomics\", based on the animations \"Modulation of gene expression by nutrients and bioactive food compounds\" and \"Nutrigenomics and risk reduction of cancer,\" for scientific dissemination of nutrigenomics for professionals in the food and nutrition and health areas. To develop the digital learning material, we used the scheme that is comprised by the following steps: analysis and planning, modeling, implementation, evaluation and maintenance. The animations were produced with the PowerPoint 2007 and EM Video Converter softwares. We evaluated the digital educational materials through a questionnaire provided to 20 professionals (nutritionists, pharmacists, food engineers, biologists, chemist, dentist and zootechnist) registered or not in Graduate Programs from University of Sao Paulo in the areas of food and nutrition and health. Over 80% of participants strongly agreed or agreed with the following statements: \"The digital educational material is easy to handle\", \"The use of this resource type presents advantages over text\", \"The digital learning material brought new knowledge on Nutrigenomics\" , \"The concepts are clearly exposed\", \"My previous knowledge was sufficient to learn from the digital learning material\", \"The texts are written with clarity\", \"The animations help to understand the exhibited phenomena\", \"I learned a lot about nutrigenomics fundamentals with the digital learning materials\". We conclude that the digital learning material \"Fundamentals of Nutrigenomics\" represents a useful tool for scientific dissemination of nutrigenomics for professionals in the field of food and nutrition and health.

Estudos têm mostrado que a atividade da enzima glutationa peroxidase (GPx) se encontra reduzida na presença do polimorfismo de nucleotideo único (SNP) Pro198Leu no gene que codifica para a GPx1. Associado a isso, polimorfismos na região promotora do gene do fator de transcrição Nrf2, o qual se liga ao elemento de resposta antioxidante na via de expressão de genes de enzimas antioxidantes, também pode alterar a expressão gênica da GPx1. Como o mineral selênio faz parte do sítio catalítico desta enzima antioxidante, muitos estudos têm associado o estado nutricional relativo a este nutriente com doenças relacionadas ao estresse oxidativo, como o diabetes mellitus tipo 1 (DM1). Nesse sentido, este estudo visou avaliar a presença dos SNPs Pro198Leu e -617 C/A no Nrf2, bem como da expressão gênica da GPx1 em pacientes com diabetes mellitus tipo 1 e relacioná-los com marcadores do estado nutricional relativo ao selênio e de estresse oxidativo, comparados com um grupo controle sem a doença. Este é um estudo caso e controle, constituído por dois grupos experimentais, um grupo composto por 77 pacientes com diabetes mellitus tipo 1 (DM1) atendidos no Setor de Endocrinologia do Hospital das Clínicas, com idade entre 10 e 19 anos, de ambos os gêneros, e um grupo controle (GC) constituído por 74 indivíduos da mesma faixa etária, os quais relataram ausência de doenças crônicas. Foi realizada avaliação antropométrica e da ingestão alimentar. Além disso, foram determinados parâmetros bioquímicos de status de selênio, controle glicêmico (glicose sérica, HbA1c), atividade enzimática, concentração de malondialdeído e 8-isoprostanos. A determinação dos SNPs e da expressão gênica foi realizada por PCR em tempo real. O grupo DM1 apresentou média de idade de 15,9 anos e o GC de 13,4 anos. A concentração de glicose sérica e HbA1c foi significativamente diferente entre os grupos (p<0,001). As frequências dos genótipos do SNP da GPx1 para o grupo DM1 e GC foram, respectivamente, 60% e 61% (CC), 30% e 32% (CT), 10% e 7% (TT),estando em equilíbrio gênico. A concentração de selênio no plasma foi significativamente maior no grupo DM1 e, ao avaliar essa concentração de acordo com genótipos, observou-se menor concentração de selênio no plasma no genótipo TT no grupo controle (p<0,05). A expressão gênica da GPx1 não apresentou diferença estatística entre os grupos nem entre os genótipos. O mesmo foi observado quanto à concentração de 8-isoprostanos. No entanto, a atividade das enzimas GPx e SOD assim como a concentração de MDA foram significantemente maiores no grupo DM1 (p<0,05). O estado nutricional de todos participantes em relação ao selênio estava deficiente. Foi observada correlação entre a concentração de selênio no plasma e nos eritrócitos e a atividade da GPx, assim como foi observada maior atividade enzimática e concentração de MDA no grupo DM, sem apresentar diferença na distribuição segundo os alelos estudados. Desta forma, pode-se concluir que independentemente da doença todos os indivíduos apresentaram deficiência de selênio. Em ambos os grupos, foi observada maior peroxidação lipídica, na presença do alelo variante T, o que pode indicar alteração da proteção antioxidante. No entanto, a presença do alelo variante nos SNPs avaliados não apresentaram influencia sobre a expressão gênica. / Studies have shown that the activity of glutathione peroxidase (GPx) is reduced in the presence of single nucleotide polymorphism (SNP) in Pro198Leu encoding GPx1 gene. Beyond that, polymorphism of the transcription factor Nrf2 gene promoter, which binds to the antioxidant response element in the pathway of antioxidant enzymes gene expression, may also alter gene expression of GPx1. As mineral selenium is part of the catalytic site of this antioxidant enzyme, many studies have associated the nutritional status of this nutrient with oxidative stress diseases, such as type 1 diabetes mellitus (DM1). Thus, this study aimed to evaluate the presence of Pro198Leu and -617 C / A in Nrf2 SNPs, as well as GPx1 gene expression in patients with type 1 diabetes mellitus, and to associate them with nutritional status of selenium and stress oxidative markers, comparing with a control group without the disease. This is a case-control study, compound of two groups, one group containing 77 patients with type 1 diabetes mellitus (DM1) from the Service of Endocrinology of Hospital das Clinicas, aged between 10 and 19 years, of both genders, and a control group (CG) was composed for 74 individuals of the same age, who reported no chronic diseases. Anthropometric and dietary intake assessment was performed. In addition, the biochemical parameters of selenium status, glycemic control (serum glucose, HbA1c), enzyme activity, concentration of malondialdehyde and 8-isoprostane were determined. The determination of SNPs and gene expression was performed by real-time PCR. The DM1 group had a mean age of 15.9 years and 13.4 years for the CG. The concentration of serum glucose and HbA 1c were significantly different between groups (p <0.001). The genotype frequencies of the GPx1 SNP for DM1 and control group were, respectively, 60% and 61% (CC), 30% and 32% (CT), 10% and 7% (TT), which is in genetic equilibrium. The selenium concentration in plasma was significantly higher in DM1 group. To assess the selenium concentration according to genotypes, we observed lower plasma concentration in TT genotype in the control group (p <0.05). The GPx1 gene expression showed no statistical difference between groups or between genotypes. The same result was observed for the 8-isoprostane concentration. However, GPx and SOD activity and MDA concentration were significantly higher in DM1 (p <0.05). The nutritional status of all participants in relation to selenium was deficient. Correlation between selenium concentration in plasma and erythrocytes and GPx activity, as well as higher enzyme activity and MDA concentration in the DM1 group were observed, with no significant difference in the distribution according to studied alleles was observed. The presence of the variant allele in the SNPs evaluated showed influence neither on gene expression, nor on the activity of GPx. Thus, it can be concluded that, regardless of the disease, all subjects had low nutritional status of selenium, without genotype influence, and, as expected, oxidative stress was increased in individuals with DM1, as demonstrated by laboratory tests.

No description available.