Neste trabalho avaliamos se as Natterinas (NTR), proteínas majoritárias presentes no veneno do peixe peçonhento T. nattereri, são as responsáveis pela a ação inibitória do recrutamento de leucócitos durante a inflamação e os mecanismos moleculares envolvidos neste processo. Utilizando análises de microscopia intravital tratamos os animais com as NTR e 6 h depois induzimos o rolamento de leucócitos com KC, LPS ou agonista de PAR-4 sobre a microcirculação. Os dados mostraram que o tratamento com as NTR inibem o rolamento e a migração de leucócitos induzido pela KC e pelo LPS. Além disso, o efeito inibitório das NTR depende parcialmente da sua atividade proteásica e não está relacionado com a clivagem proteolítica da quimiocina KC ou com a indução de reguladores endógenos como IL-10, corticóides, a enzima HO-1 ou IL-1Ra. O efeito inibitório parece ser dependente da ativação da sinalização TLR/MyD88 e PI3K e das proteínas serina/treonina fosfatases, contribuindo de forma significativa na sobrevida de animais induzidos a endotoxemia por LPS. / In this work we evaluated whether Natterins (NTR) the majority proteins present in the venom of Thalassophryne nattereri venomous fish are responsible for the inhibitory action of leukocyte recruitment during inflammation and the molecular mechanisms involved in this process. Using intravital microscopy analysis we treated the animals with NTR and 6 h after we induced the leukocyte rolling by topical of KC, LPS or agonist of PAR-4 on the microcirculation. The data showed that treatment with NTR inhibited the leukocyte rolling and migration induced by LPS and KC. Moreover, the inhibitory effect of NTR depends partly on its proteinase activity and is not related to the proteolytic cleavage of the chemokine or KC nor with the induction of endogenous regulators such as IL-10, corticoids, the HO-1 or IL-1Ra. The inhibitory effect is dependent on the activation of PI3K and TLR/MyD88 signaling and protein serine/threonine phosphatases, contributing significantly to the survival of animals endotoxemia induced by LPS.

Submitted by Rosivalda Pereira (mrs.pereira@ufma.br) on 2017-05-09T19:48:14Z No. of bitstreams: 1 MayanaLuana.pdf: 2132103 bytes, checksum: ec28c6abf33e625325d7947c9311143a (MD5) / Made available in DSpace on 2017-05-09T19:48:14Z (GMT). No. of bitstreams: 1 MayanaLuana.pdf: 2132103 bytes, checksum: ec28c6abf33e625325d7947c9311143a (MD5) Previous issue date: 2017-02-23 / CAPES / The Portuguese man-of-war Physalia physalis (Linnaeus, 1758) is recognized for causing poisoning in bathers, mainly in the north and northeast regions of Brazil. In Maranhão, poisonings by man-of-war are common on the beaches of the capital São Luís. But action to prevent and promote health are still deficient. This study aimed to analyze the distribution of the occurrence of Portuguese man-of-war and envenomations on the beaches of São Marcos and Calhau in São Luís, Maranhão between 2015 and 2016. The data was provided by the active search of the animals and geographical marking with GPS (Global Positioning System), geoprocessed by Qgis software version 16.1. The kernel density estimator was used to identify the agglomerations of the events. A specific questionnaire was applied with 66 people poisoned by P. physalis and registered the location of the accident, month, year and emergency care measure used. The animals are distributed throughout the beaches of São Marcos and Calhau, with greater agglomeration in Calhau. The highest density location of P. physalis coincided with the location with the highest occurrence of poisonings. Most of the poisonings occurred in people living in the city of São Luís, in the months of the dry period, with greater occurrence in the beach of São Marcos. The most common first-aid used was the application of vinegar (acetic acid). We concluded that the poisonings occur in the parts of the urban beaches of São Luís that presented great density of P. physalis. In order to improve poison control actions, we suggest signaling with signs warning the risk areas of poisoning, and include the geographical location of the accident in the notification form. / A caravela portuguesa Physalia physalis (Linnaeus, 1758) é reconhecida por provocar envenenamento em banhistas, principalmente nas regiões norte e nordeste do Brasil. No Maranhão, são comuns envenenamentos por caravelas nas praias da capital São Luís, porém as ações de prevenção e promoção â saúde ainda são deficientes. O objetivo desse estudo foi analisar a distribuição da ocorrência de caravelas e dos envenenamentos humanos nas praias urbanas de São Marcos e do Calhau nos anos de 2015 e 2016. Os dados foram obtidos por meio da busca ativa dos animais e marcação da coordenada geográfica com um aparelho de GPS (Sistema de Posicionamento Global); e geoprocessados pelo software Qgis versão 16.1. O estimador da densidade de Kernel foi utilizado para identificar as aglomerações dos eventos. Um questionário específico foi aplicado com 66 pessoas envenenadas pela P. physalis sendo registrados o local do acidente, mês, ano e medida de pronto-atendimento utilizada. As caravelas estão distribuídas em toda a extensão das praias de São Marcos e Calhau, com maior aglomeração no Calhau. Os locais de maior densidade de caravelas coincidiram com os locais de maior ocorrência de envenenamentos. A maioria dos envenenamentos ocorreu em pessoas residentes na cidade de São Luís, nos meses do período de seca, com maior ocorrência na praia do São Marcos. A medida de pronto-atendimento mais utilizada foi a aplicação de vinagre (ácido acético). Concluímos que os envenenamentos ocorrem nos trechos das praias urbanas de São Luís que apresentaram grande densidade de colônias de caravelas. Para melhoria das ações de controle dos envenenamentos sugerimos a sinalização com placas alertando as áreas de risco de envenenamento, e inclusão da localização geográfica do acidente na ficha de notificação.

Orientador: Benedito Barravieira / Coorientador: João Pessoa Araújo Júnior / Banca: Reinaldo José da Silva / Banca: Francisco Luis Franco / Resumo: / Abstract: / Mestre

Previous publications comprise main text of thesis / Includes bibliographical references / 1 v. (various pagings) : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (M.D.)--University of Adelaide, Dept. of Pathology, 1988

This study was undertaken as a result of the occurrence of a large number of deaths among the local Black population from the use of herbal medicines prepared from the rootstock of Callilepis laureola known to the Zulus as impila. The salient clinico-pathological features in these cases were hypoglycaemia, centrilobular zonal liver necrosis and acute renal tubular necrosis. The purpose of this study was to investigate fully the clinical, biochemical and pathological aspects of the toxicity produced by Callilepis laureola (impila). The first part of the investigation consisted of an assessment of all cases of death due to acute liver necrosis diagnosed by necropsy at King Edward VIII Hospital, Durban. A review of clinical and necropsy records of 21687 consecutive post-mortems performed on Black patients during a 20 year period showed that acute liver necrosis was the major contributing cause of death in 447 patients. In 263 cases the hepatic lesion was centri lobular zonal necrosis with associated acute tubular necrosis (Group A); while in 184 cases the I iver necrosis was of the massive or submassive type (Group B). A comparative assessment of these two groups as regards necropsy prevalence, age and sex distribution and the clinical, biochemical and pathological findings was undertaken. This study shows that the combination of hypoglycaemia, centri lobular zonal liver necrosis and acute renal tubular necrosis due to Callilepis laureola (impila) poisoning is a distinct clinico-pathological entity and differentiates this group from cases of acute massive and submassive liver necrosis resulting in most cases from fulminant viral hepatitis. In the search for the toxic components of the root of Callilepsis laureola several compounds were isolated. These were atractyloside, carboxyatractyloside, two thymol related oils and a carbohydrate. The thymol related oils as well as the carbohydrate were found to be non-toxic in laboratory rats. The crude methanol extract of the root of Callilepsis laureola, when injected intraperitoneally into laboratory rats, produced centrilobular zonal liver necrosis and acute renal tubular necrosis, the lesions identical to those seen in patients who had died after intake of impila prescribed by witchdoctors and other dispensers of herbal medicines. On the other hand intraperitoneal injections of the purified compound atractyloside caused acute renal tubular necrosis and hypoglycaemia in laboratory rats but failed to produce liver necrosis. Carboxyatractyloside also failed to cause liver necrosis. This indicated that there may be at least two toxins contained in the rootstock of Callilepsis laureola, one causing the liver lesion and the other (atractyloside) causing nephrotoxicity and hypoglycaemia. Repeated attempts at isolating the hepatotoxin have failed; the liver toxin or toxins being lost during the process of extraction and purification. Identification of the hepatotoxin awaits further investigation. It is possible that the liver necrosis may be caused by a metabolite or that it may be a synergistic effect of two or more compounds. / Thesis (MD)-University of Natal, 1983.

Toxicology is a scientific discipline dealing with the influences of dangerous substances on living organisms. The negative effects of some substances have been known since the prehistoric times and the human race has had to face them until today. The impact of these substances on human organisms may be caused either deliberately (e. g. misuse of chemical substances during war conflicts) or unintentionally (e. g. intoxication by poisonous gases released from fire). The current knowledge of the field of toxicology enable to characterise the dangerous substances, to define their effective, toxic and lethal doses and concentrations and to give first aid to the people affected. The knowledge of the first aid procedure in case of intoxication of poisonous gases is crucial for the rescue of human life and moderation of the toxic effects. In connection to the universal first aid procedure, i. e. the patient's rescue out of the contaminated area and calling of the rescue service, the person affected has to receive an adequate immediate pre-hospital care. Based on these findings, procedures used in cases of intoxication in pre-hospital emergency care and emergency medicine are listed. The aim of the theoretical part of this diploma thesis is a comprehensive overview of current theoretical and professional knowledge in the field of chemistry, toxicology and emergency medicine. This knowledge is subsequently applied to the overall management of pre-hospital emergency care for patients intoxicated with toxic gases. The research part includes a retrospective statistical analysis of the number of cases of carbon monoxide intoxication in Vysocina Region, application of theoretical knowledge in the form of analyses of cases of intoxication and creation of a preventive programme with the help of a questionnaire survey which asked the public about their awareness of the risks and dangers of carbon monoxide. In addition, educational material was created. The thesis also tried to answer the research question "How serious is poisonous gas intoxication in pre-hospital emergency care and emergency medicine?" From the point of view of pre-hospital emergency care, the average daily death rate caused by poisonous gas intoxications in the Vysocina Region is four patients per year. When this number is compared with the total number of patients' deaths, it shows a less serious problem. The most common source of carbon monoxide poisoning turned out to be a gas appliance. The rescuers recorded the highest number of carbon monoxide detector activations in 2015. Informing the public in the form of a questionnaire survey has clearly shown that there is persisting confusion of carbon monoxide properties with carbon dioxide ones. This diploma thesis is aimed at specialists providing pre-hospital emergency care i. e. health rescue services and also fire rescue services for the purpose of statistical comparisons with other providers and subjects, or for educational purposes in the context of crisis preparedness. In addition, the work is recommended to the general public to ensure greater awareness of poisonous gas intoxications. This thesis brought a comprehensive overview of this issue which can be used for the purpose of informing, educating and creating the basis for further comparison.

Made available in DSpace on 2015-10-06T13:03:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-02-25. Added 1 bitstream(s) on 2015-10-06T13:18:37Z : No. of bitstreams: 1 000849914.pdf: 1571481 bytes, checksum: a13d0db3f8c4f4bc8ed5e8460b1d9f2d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Made available in DSpace on 2014-06-11T19:24:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-10Bitstream added on 2014-06-13T18:20:27Z : No. of bitstreams: 1 gasparotto_vpo_me_botfm.pdf: 823732 bytes, checksum: 72bbebb42d3239b78a28393a5aa3b0b7 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Universidade Estadual Paulista (UNESP) / O estudo avaliou a viabilidade in vitro do biomaterial “Selante de Fibrina derivado de veneno de serpente” (SF), como arcabouço para células tronco mesenquimais (CTMs) de ratos. O SF é um material caracterizado como adesivo biológico, e foi produzido e fornecido pelo Centro de Estudos de Venenos e Animais Peçonhentos, CEVAP, Brasil. As CTMs foram coletadas a partir da medula óssea de fêmures e tíbias de ratos e foram caracterizadas por meio de citometria de fluxo com auxilio de marcadores positivos: CD 44 e CD 90 (CTMs) e marcador negativo: CD 34 (células tronco hematopoiéticas). Cultivos foram induzidos para diferenciarem-se em linhagens específicas (osteogênico, condrogênico e adipogênico). Para avaliação do crescimento in vitro e a viabilidade celular em conjunto ao biomaterial foram utilizadas microscopia óptica de luz invertida, microscopia de fluorescência e microscopia eletrônica de varredura e transmissão. O SF em contato com as CTMs não induziu a diferenciação espontânea para as linhagens osteogênica, condrogênica e adipogênica. As diferentes técnicas de avaliação microscópica utilizadas mostraram que o SF foi capaz de realizar a captura e manutenção das CTMs e houve interação das células com o interior e superfície do biomaterial. Portanto, a coleta, o cultivo e a caracterização das CTMs de ratos foram possíveis. O SF mostrou-se eficiente como arcabouço biológico e interagiu com as células tronco mesenquimais mantendo-as viáveis, oferecendo-se como uma ferramenta de uso clínico-cirúrgico alternativa para processos regenerativos visando terapias mais eficientes / This study evaluated the in vitro viability of biomaterial Fibrin Sealant (FS) derived from snake venom as a scaffold for rat mesenchymal stem cells (MSCs). The FS is characterized as a biological adhesive material, and is produced and was supplied by the Center for the Study of Venoms and Venomous Animals, CEVAP, Brazil. MSCs were collected from the bone marrow of femurs and tibias of rat and were characterized using flow cytometry with CD 44 and CD 90 positive markers (MSC) and CD34 negative marker (mononuclear stem cells). Cultivations were induced to differentiate into specific cell lineage (osteogenic, chondrogenic and adipogenic). To evaluate the in vitro growth and cell viability with the biomaterial were used inverted light optical microscopy, fluorescence microscopy and scanning electron microscopy and transmission. The SF did not cause the spontaneous differentiation in contact with MSCs to osteogenic, chondrogenic and adipogenic lineage. Different microscopy techniques showed that the SF was able to accomplish the capture and maintenance of MSCs and there was interaction with the cell interior and surface of the biomaterial. Finally, the collection, cultivation and characterization of rat MSCs were possible. The SF was effective as a biological scaffold and interacted with the MSCs keeping them viable offering itself as a tool for clinical and surgical alternative providing clinical and surgical therapies more efficient for regenerative processes

Made available in DSpace on 2016-12-08T16:24:20Z (GMT). No. of bitstreams: 1 PGCA15MA166.pdf: 955554 bytes, checksum: a7513a7a99db2af80031aee98a3385bf (MD5) Previous issue date: 2015-02-20 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Senecio oleosus is a plant of Astereacea family found in the South and Southeast of Brazil. Species of this genus are recognized to produce liver damage in different animal species, including in human. This study aimed to evaluate the toxicity of S. oleosus in poultry (Gallus gallus domesticus). Green leaves of S. oleosus were collected in the city of Ponte Alta/SC, dried in the shadow, crushed, mixed with feed and fed to four groups of 10 broilers. Group 1 and Group 2 received single doses of 5g/kg and 20g/kg, respectively. Group 3 were given daily doses of 1g/kg for 20 days (amounts corresponding to green plant) and Group 4 (Control) received feed free of S. oleosus. Five broilers from each group were necropsied 30 days after the beginning of the experiment and the five remaining broilers were necropsied 60 days after the start of supply with the plant. Group 1 showed no gross and microscopic changes. In Groups 2 and 3 were observed yellowish color and increase in the volume of the liver, ascites and, microscopically, there was hepatotocyte vacuolation and megalocytosis, fibrosis and biliary epithelium hyperplasia / Senecio oleosus é uma planta da Família Asteraceae encontrada nas regiões Sul e Sudeste do Brasil. Plantas desse gênero são conhecidas por produzirem lesões hepáticas em diferentes espécies de animais, inclusive no homem. Para avaliar a toxicidade do S. oleosus foram conduzidos experimentos em aves (Gallus gallus domesticus). Folhas verdes de S. oleosus foram coletadas no município de Ponte Alta/SC, secadas a sombra, trituradas, misturadas a ração e fornecidas para quatro grupos de 10 frangos de corte. O Grupo 1 e o Grupo 2 receberam doses únicas de 5g/kg e 20g/kg, respectivamente. Ao Grupo 3 foram fornecidas doses diárias de 1g/kg por 20 dias (valores correspondentes a planta verde) e o Grupo Controle recebeu ração livre de S. oleosus. Cincos frangos de cada grupo foram submetidos à necropsia aos 30 dias do início do experimento e os cinco restantes foram submetidos a necropsia aos 60 dias após o início do fornecimento da planta. As aves do Grupo 1 não apresentaram alterações macro e microscópicas. Na necropsia das aves dos Grupos 2 e 3 foi encontrado principalmente aumento de volume e coloração amarela do fígado e ascite. À microscopia foi observado megalocitose, vacuolização de hepatócitos, fibrose e hiperplasia de epitélio biliar

Made available in DSpace on 2016-12-08T16:24:22Z (GMT). No. of bitstreams: 1 PGCA10MA180.pdf: 4478459 bytes, checksum: 6a32501ee3779e2d35231ab3dac3f5c3 (MD5) Previous issue date: 2010-01-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A disease of cattle with clinical and pathological presentation of acute myopathy was studied in the cities of Bom Jesus and Xanxerê in the State of Santa Catarina. Clinically, the disease was characterized by difficulty in walking, recumbency and death within 2 to 13 days after the onset of clinical symptoms. At necropsy were observed pale areas in the skeletal muscles mainly in the muscles of the hindquarters and the scapular area. The main microscopic lesions were hyaline degeneration and necrosis with fragmentation of the fibers, accompanied by mononuclear cell infiltrates. In the property there was great amount of S. obtusifolia that demonstrated that was consumed by the animals. The disease was reproduced experimentally in cattle fed with green leaves and pods of Senna obtusifolia in daily doses of 8g/kg (4 days), 10 g / kg (5 days), 15 g / kg (3 days) and a single dose 30g / kg body weight. This study aimed to evaluate the epidemiological and clinical lesions of a disease that affects the skeletal muscles of cattle and experimentally reproduce the disease through the administration of leaves and pods of Senna obtusifolia / Uma doença de bovinos com quadro clínico patológico de miopatia aguda foi estudada nos municípios de Xanxerê e Bom Jesus, SC. Clinicamente a enfermidade caracterizou-se por dificuldade em caminhar, decúbito esternal e morte entre dois a 13 dias após o inicio dos sinais clínicos. Um dos bovinos se recuperou e voltou a andar. Na necropsia foram observadas áreas pálidas na musculatura esquelética principalmente nos músculos dos membros posteriores e da região escapular. Ao exame microscópio, a principal lesão foi degeneração hialina e necrose com fragmentação das fibras, acompanhadas de infiltrado mononuclear. Na propriedade havia grande quantidade de S. obtusifolia com sinais de ter sido ingerida A doença foi reproduzida experimentalmente em bovinos com a administração de folhas e vagens verdes de Senna obtusifolia em doses diárias de 8g/kg (4 dias), 10g/kg (5 dias), 15g/kg (3 dias) e em dose única 30g/kg de peso vivo. O objetivo do presente trabalho foi avaliar os aspectos epidemiológicos clínicos e lesionais da enfermidade que afeta a musculatura esquelética dos bovinos e reproduzir experimentalmente a doença através da administração de folhas e vagens de Senna obtusifolia