Baigiamojo darbo pavadinimas: regos terapijos įtaka vaikų akių refrakcijai. Darbo tikslas: išanalizuoti regos terapijos įtaką vaikų akies refrakcijai. Tyrimas atliktas: Akių pratimų kabinete, Gargždų mieste 2012-2013 metais. Metodai: 2012 - 2013 metais stebėti 25 (4 – 9 metų) mokyklinio ir ikimokyklinio amžiaus vaikai lankantys akių pratimų kabinetą Gargždų mieste. Vertinama kaip įtakojo vaiko regą gydytojo oftalmologo paskirtas gydymas, bei įvairios lavinimo priemonės. Darbo apimtis: Darbą sudaro 53 puslapiai. Pirmoje dalyje apžvelgta mokslinė literatūra. Antroje dalyje nagrinėjami regos terapijos prietaisai, regėjimą gerinantys pratimai atskiroms refrakcijos ydoms. Pateikiamos rekomendacijos, bei darbo išvados. / The theme of the final work: Vision therapy influence children's refractive. The aim: To analyze the influence on children's vision therapy eye refraction.The research was conducted: Eye exercises cabinet Gargždai in 2012-2013. Methods: 2012 - 2013 year track 25 (4-9 years) and pre-school-age children attending eye exercises Gargždai office in the city. Seen as influenced by the child's vision doctor ophthalmologist prescribed treatment, and various development tools. Scope of work: The work consists of 53 pages. In the first part of an overview of the scientific literature. The second part deals with vision therapy devices, improving eyesight exercises for individual refractive defects. The recommendations and conclusions of the work. Results: after the 10th second. exercise course visual acuity improved by 1-2 rows 80 percent of the children three or more lines of 8 percent. 12 percent. vision has not changed.

Submitted by Andre Moraes Queiroz (andre.moraesqueiroz@ufpe.br) on 2015-04-14T14:30:37Z No. of bitstreams: 2 Dissertaçao Carolina Neves Correia.pdf: 5310409 bytes, checksum: 06fd884bd1cc5beab0d792b693d0b03f (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) / Made available in DSpace on 2015-04-14T14:30:37Z (GMT). No. of bitstreams: 2 Dissertaçao Carolina Neves Correia.pdf: 5310409 bytes, checksum: 06fd884bd1cc5beab0d792b693d0b03f (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2013 / FINEP / Estudos de expressão gênica diferencial associados a perfis metabólicos de plantas submetidas a um estresse alvo são abordagens valiosas na compreensão da tolerância vegetal. Por sua vez, proteínas quinases são importantes componentes da sinalização celular, catalisando a transferência do grupo fosfato de uma molécula de ATP para um substrato, em processo chamado fosforilação, com participação ativa na aclimatação às mudanças ambientais. O presente trabalho teve como objetivo comparar in silico perfis de transcrição, de unitags SuperSAGE diferencialmente expressas e anotadas como prováveis quinases, de genótipos de cana-de-açúcar tolerantes e sensíveis ao déficit hídrico (24 h de supressão de rega). Para tanto, sequências expressas (EST) de gramíneas, de diversos bancos de dados públicos, similares às unitags (análises BLASTn), foram caracterizadas em termos de Ontologia Gênica, que conjuntamente com as anotações das EST permitiram identificar e classificar as quinases em famílias. Através dos respectivos números de Classificação Enzimática (EC), mapas metabólicos foram gerados, permitindo comparar os grupos de genótipos contrastantes (tolerantes e sensíveis ao estresse). Foram observadas 539 prováveis quinases, que distribuídas em 41 famílias e em 18 vias metabólicas, quando associadas às unitags, permitiram discuti-las com base em oito classes hierárquicas funcionais. Importantes diferenças na expressão desses transcritos mapeados como quinases, entre os grupos de genótipos tolerantes e sensíveis, após 24 h de supressão de rega, foram observadas neste trabalho, contribuindo para um melhor entendimento da percepção do estresse aplicado, podendo ser útil ao melhoramento genético da cana-de-açúcar.

La capacité de Brucella suis, un microorganisme strictement aérobique, a s'adapter aux taux d'oxygène faible est un processus essentiel pour la virulence et la persistance bactérienne. Le manque d'oxygène est une condition hostile à laquelle les bactéries sont confrontées lors de la pénétration de l'hôte et pour établir leur niche replicative et la phase de persistance. Cette bactérie possède plusieurs mécanismes par laquelle elle s'adapte à cette condition. Elle peut utiliser un régulateur transcriptionel de la famille de FnrN dépendant de l'oxygène, deux cytochromes oxydases de haute affinité pour l'oxygène et une voie complète de denitrification pour résister au manque d'oxygène. Ce travail a démontré que la respiration oxydative et la denitrification peuvent être simultanément utilisés par B. suis sous microaerobiosis. RegBA, un système à deux composants chez B. suis, a été aussi identifié nécessaire dans l'adaptation bactérienne au manque d'oxygène. Ce dernier a été démontré à coordonner le contrôle des systèmes respiratoires précédemment évoqués. Un schéma de régulation global chez B. suis des voies respiratoires par le régulateur transcriptionel RegA a été suggéré : lors de la variation de l'état redox, la cytochrome bd oxidase jouerait un rôle dans la transmission d'un signal à RegB le senseur de la histidine kinase. De plus, RegA a été identifié essentiel pour la persistance de B. suis in vivo chez la souris dans les organes avec des teneurs faible en oxygène. RegA est supposé être impliqué dans l'installation de la phase de persistance bactérienne durant l'infection chronique. Cette étude a aussi identifié le rôle potentiel de RegA dans la régulation de nombreux gènes impliqués durant la phase de persistance. En utilisant une analyse transcriptomique, comparant les taux d'hybridation chez les souches sauvage et muté dans un modèle in vitro qui imite les conditions d'une infection chronique correspondant a un manque de nutriment et d'oxygène, 447 gènes avec un taux d'hybridation ≥ 2, ont été détectés réguler par RegA. Chez la souche sauvage, 45% et 55 % des gènes étaient régulés et réprimés par RegA chez la souche sauvage, respectivement. 14% des résultats du transcriptome a été choisi pour la validation génétique par RT-qPCR. RegA induit l'expression de gènes impliqués dans le métabolisme d'énergie y compris des gènes de la respiration oxidative, ce qui confirme qu'il interagit dans l'adaptation bactérienne au manque d'oxygène. RegA réprime des gènes impliqués dans la réplication d'ADN, la biogenèse de l'enveloppe et la division cellulaire, de même certains gènes dans le métabolisme d'énergie, ce qui suggère son effet sur la multiplication et l'adaptation bactérienne à l'hypoxie qui existe durant la phase de persistance. RegA a été démontré a réprimer les facteurs de virulence l'operon virB ainsi que son régulateur VjbR. De plus, cette étude a évalué le rôle de deux gènes BR1614 et BR1510 régulés par RegA et impliqués dans le métabolisme des acides gras. Dans les expériences in vivo chez la souris ont démontré que les deux gènes sont essentiels pour la survie, la multiplication et la persistance bactérienne. En conclusion, RegA régule, directement et indirectement, l'expression de gènes qui codent pour la traduction, la transcription, la production d'énergie et la conversion, la réparation d'ADN et de protéine. Ces résultats suggèrent un rôle majeur pour RegA dans la persistance bactérienne pendant la brucellose. 12% du génome de B. suis est sous le contrôle de RegA ce qui indique qu'il est un régulateur global comme son PrrA d'homologue dans Rhodobacter sphearoides. / The capacity of Brucella suis, a strictly aerobic microorganisms, to adapt to low oxygen level is of high importance as it is a required and an essential process for bacterial establishment of virulence and persistence. Oxygen deficiency is a hostile condition to which bacteria are faced when they penetrate the host and reach their replicative niche as well as the persistence phase. This bacterium possesses several mechanisms that answer remarkably to this condition. It can use an oxygen-dependent transcriptional regulator of the FnrN family, two high-oxygen-affinity terminal oxidases, and a complete denitrification pathway to resist various conditions of oxygen deficiency. This work has demonstrated that the oxidative respiration and denitrification can be simultaneously used by B. suis under microaerobiosis. RegBA, a two component systems in B. suis, was also identified to be necessary in bacterial adaptation to oxygen deficiency as it was demonstrated to coordinate the control of the respiratory systems mentioned previously. A scheme for global regulation of B. suis respiratory pathways by the transcriptional regulator RegA was suggested: under redox variation, the cytochrome bd ubiquinol oxidase would play a role in the transmission of a signal to the histidine sensor kinase RegB. RegA in addition was found to be essential for B. suis persistence in vivo in mice within low oxygenated organs. RegA is thus assumed to be involved in the establishment of bacterial persistence during chronic infections. This study also investigated the potential control of RegA in the regulation of numerous genes during the persistence phase. By using a microarray assay comparing wild-type and ∆regA mutant strains, in an in vitro model that mimic the conditions of a chronic infection corresponding to nutrient and oxygen deficiency, 447 genes with a cutoff of the level of hybridization intensities ≥2, were detected regulated by RegA. In the wild-type strain, 45% and 55 % of the genes were up-regulated and down-regulated in wildtype strain, respectively. 14% of the microarray results were selected for genetic validation by RT-qPCR. RegA induced the expression of some genes involved in energy metabolism including the oxidative respiratory genes confirming that it interacts in bacterial adaptation to oxygen deficiency. RegA down-regulated genes involved in DNA replication, cellular division cell envelope biogenesis as well as certain genes in energy metabolism suggesting its impact on bacterial multiplication and adaptation to hypoxia as it enters into the persistence phase. RegA was also found to down-regulate virulence factors such as the virB operon as well as its regulator VjbR. Moreover, this study evaluated the role of two genes BR1614 and BR1510 regulated by RegA and found implicated in fatty acid metabolism. In vivo experiments in mice demonstrated that both genes are required for bacterial survival, multiplication and persistence. In conclusion, RegA was found to regulate, directly and indirectly, the expression of genes that encode for functions in translation, general transcription, energy production and conversion, repair of DNA and protein which represent its high importance and major role in bacterial persistence during brucellosis. 12% of the genome of B. suis is under the control of RegA which makes it a global regulator such as his homologue PrrA in Rhodobacter sphearoides.

Encystation of unicellular organisms is of considerable medical relevance since cysts are encapsulated byresilient cell walls, rendering them resistant to biocides and immune clearance. This survival strategymakes it complicated to produce effective treatment of diseases caused by many protozoan pathogens,e.g. species of Acanthamoeba which causes fatal granulomatous amebic encephalitis (GAE) and keratitis.Due to genetic limitations in most protists, the machinery of encystation is so far little understood.However, the signalling pathways can be investigated in the non-pathogenic social amoebas, Dictyostelia.In this master’s project, five genes in Polysphondylium pallidum were investigated, which might beinvolved in encystation. Research has demonstrated a relationship between encystation and the cyclicadenosine monophosphate (cAMP) signalling pathways in Dictyostelia spore formation. This indicates thatcysts are ancestral to spores, and hence are the sporulation genes: pkaC, yakA, regA, cudA and srfAselected for this study. The genes were individually knocked-out by a standard homologous recombinationapproach and the genes’ contribution to encystation was determined. Five knock-out constructs werecompleted and a preliminary analysis of the role of the intracellular cAMP phosphodiesterase RegA inPolysphondylium pallidum encystation process was performed.

Baigiamajame magistriniame darbe nagrinėjama tema – ikimokyklinio amžiaus aklųjų ir silpnaregių kalbos raidos ypatumai ( lyginamoji analizė). Sunku pervertinti kalbos plėtotės procese pagrindinių – regos ir klausos – analizatorių vaidmenį. Šių analizatorių sutrikimas labai stipriai įtakoja kalbinės veiklos vystymąsi ir suteikia jam tam tikrų ypatumų. Labai dažnai aklo iš prigimties asmens turimų sąvokų turinys kardinaliai skiriasi nuo reginčiųjų. Stokodami vieno iš pasaulio pažinimo būdų aklieji dažnai neadekvačiai vartoja turimas sąvokas. Aklas vaikas gali girdėti kalbą ir mėgdžiodamas jos mokosi. Kartais jis labiau už regintįjį yra motyvuotas ją vartoti, nes tai pagrindinis kanalas komunikuojant su kitais. Ar iš tikrųjų aklųjų kalbinė raiška skiriasi nuo normaliai matančių vaikų? Į šį klausimą bandoma atsakyti šiame magistro darbe. Tyrimo problema: ikimokyklinio amžiaus nereginčių ir silpnaregių vaikų kalbinė raida stokoja normalaus psichofiziologinio pagrindo. Šiuos trūkumus būtina kompensuoti, pasitelkus išlikusius sveikus analizatorius prieš tai tiksliai išsiaiškinus šios grupės vaikų kalbinės raidos pasiekimo skirtumus lyginant su sveikaisiais. Tyrimo objektas: ikimokyklinio amžiaus aklųjų ir silpnaregių bei gerai matančių vaikų kalbos raida ( lyginamoji analizė). Tyrimo tikslas: ištirti ikimokyklinio amžiaus aklųjų ir silpnaregių kalbos raidos ypatybes. Tyrimo uždaviniai: 1. Išanalizuoti psichologinę ir pedagoginę literatūrą apie aklųjų ir silpnaregių kalbinę raidą... [toliau žr. visą tekstą] / SUMMARY The final master‘s study deals with the peculiarities in lingual development of the blind and poor-sighted children at preschool age (the comparative analysis). It is difficult to overestimate the role of the main analyzers, i.e. sight and hearing in the process of lingual development. The disorder of these analyzers affects very strongly the development of lingual activities and renders certain peculiarities to it. Very often the contents of concepts of a person, who is blind by nature, cardinally differ from that of people with normal sight. Lacking one technique for world cognition, the blind persons often inadequately use the concepts available. The sightless child can hear the language and learn it by imitation. Sometimes he is more motivated to use it than a seeing person, since it is the main channel for communication with others. Does really the lingual expression of the blind children differ from that of normally sighted? In this master’s study the attempts were done to answer this question. Problem of study. The lingual development of blind and poor-sighted preschoolers lacks normal psycho-physiological substantiation. These shortcomings should be compensated with help of the remaining healthy analyzers. But before it the differences in achieved lingual development between the children of this group and healthy ones should be find out. Object of study: lingual development in blind and poor-sighted children at preschool age (the comparative analysis). ... [to full text]

Šių dienų miestai darosi itin dideli, o automobilių kiekis juose dar didesnis. Didelis automobilių kiekis kelia didžiules transporto spūstis, o dėl jų miesto gyventojų susisiekimas pasidaro svarbi problema. Transporto spūstys taip pat yra milžiniška taršos problema, kuri sukelia Globalinį atšilimą ir sveikatos sutrikimus. Šiame Bakalauro darbe yra mažinamos transporto spūstys skaičiuojant į sankryžas atvažiuojančius automobilius ir generuojant skirtingus šviesoforo signalų ciklo laikus atsižvelgiant į jų skaičių. Darbas susideda iš kelių dalių, tarp kurių programiniai automobilių skaičiavimo sprendimai, mikrovaldiklio pasirinkimas, šviesoforo signalų valdymo programos kūrimas, kameros prijungimas. / Nowadays modern cities becoming very large and the quantity of vehicles in them are even larger. The large number of vehicles leads to huge traffic jams and because of them communication for residents became a serious problem. Traffic jams are also a major pollution problem which causes Global warming and health illness. In this Bachelor's Thesis we are reducing traffic jams by counting the amount of vehicles approaching crossroads and generating different cycle times of the traffic light signals. The Thesis consists of several parts including possible programming solutions for vehicle count, the choice of microcontroller, creation of traffic light signals program, camera connection.

Dissertação apresentada para obtenção do grau de Mestre em Tecnologia Multimédia, na Faculdade de Engenharia da Universidade do Porto, sob a orientação do Professor António Augusto de Sousa e do Engº. José Miguel Losa

Eglė Meškauskaitė. Cognitive maturity for school of preschool-aged children with defective sight in the context of integrated education. Scientific adviser: prof. habil.dr. Vytautas Gudonis. Vilnius Pedagogical University, Faculty of Pedagogy and Psychology, Department of Pedagogy, Branch of special education. – Vilnius, 2005. In order to determine cognitive maturity for school of preschool-aged children with defective sight in the context of integrated education 50 children with defective sight from the kindergartens of Kaunas and Vilnius, in respect of whom integrated education is applied, and 50 children with good sight from the kindergartens of Kaunas, Kulautuva and Raudondvaris have been examined. The research is based on a questionnaire which has been developed in accordance with the “General Programmes and Education Standards. Preschool Education” (2003). The data processing has revealed that the maturity of children with defective sight for school in the context of having an interest in the self, the own body, adults, knowledge of the close natural environment and the world, performance of practical tasks is not only lower than the one of children with good sight, but even higher. No differences between children with good sight and children with defective sight have been established as regards other principal capacities connected with children’s cognitive maturity for school, as set out in the general programmes and education standards. It has been established that... [to full text]

Elektronikos inžinerijos magistro darbo tema yra aktuali įvairiose šiuolaikinėse moderniųjų technologijų vystymosi ir pritaikymo srityse. Kompiuterinė rega siekia kurti algoritmus, kurie vis tobuliau leistų mašinoms atkartoti žmogaus vieną svarbiausių jutimų – regą, gebėjimą apdoroti vaizdinę informaciją, suprasti jos turinį. Šiuolaikinių atpažinimo modelių metodai yra požymiais grįsti algoritmai. Šiame darbe žmonių atpažinimui vaizde pasirinktas orientuotų gradientų histogramų (HOG) metodas. Išanalizuotas HOG metodo veikimo principas, apžvelgti kiti šio metodo pagrindu veikiantys metodai. / The theme of Master project of Electronics engineer is actual in various modern high – tech development and application domains. Computer vision develops algorithms to make machines more advanced to replicate one of the most important human senses – sight, the ability to process visual information, understand its contents. Modern methods of detection models are based on the features of algorithms. The histogram of oriented gradients (HOG) method performs the identification of people at a picture. We analyzed the principle of operating of HOG method and also took overview of few other operating methods based on HOG.

Tarptautinė apšvietimo komisija (TAK) 1931 m. akies reakcijai į spalvas aprašyti eksperimentiškai nustatytos ir pradėtos naudoti r(λ), b(λ), g(λ) funkcijos. Tačiau šių funkcijų analizinės išraiškos gana netikslios (Gauso funkcija) ir gremėzdiškos (Furje eilutė). / The thesis problem. The International Commission on Illumination (CIE) to describe eye reaction to colours in 1931, experimentally identified and began using functions r(λ), b(λ), g(λ). However, the analytic expressions of these functions are not explicit enough (the Gaussian function) and cumbersome (a Fourier series). The main aim of the thesis – to find simple analytic expressions of the functions r(λ), b(λ), g(λ), which could be adapted for practical calculations.