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Desenvolvimento, caracterização e desempenho biológico de um novo biomaterial de liberação controlada à base de carbonato de lítio aplicado ao reparo ósseo / Development, characterization and biological performance of a new biomaterial of controlled release based on lithium carbonate applied to bone repairMarques, Leonardo 09 June 2017 (has links)
Neste trabalho desenhou-se bases de desenvolvimento, caracterização e avaliação dos aspectos biológicos relacionados ao Sistema BoneLithium, idealizado a partir da associação de partículas de carbonato de lítio dispersas em matriz gel de carbopol®, com capacidade de atuar como um sistema liberador de fármacos. Metodologicamente este estudo se dividiu em quatro partes: Na primeira delas, o objetivo central foi o desenvolvimento e a caracterização do biomaterial através da manipulação farmacológica. Na segunda etapa, avaliou-se a reação tecidual em subcutâneo de ratos, na terceira a influência das partículas de lítio liberadas pelo Sistema BoneLithium no reparo ósseo através de modelos experimentais utilizando coelhos, e por ultimo, a capacidade de cicatrização de defeitos ósseos criados cirurgicamente em calvária de ratos, tratados com o biomaterial e diferentes opções de enxertos ósseos com o objetivo de comparar a eficiência do Sistema BoneLithium aos protocolos pré-existentes. Experimentalmente, avaliou-se a reação tecidual onde se utilizou 15 ratos machos divididos aleatatoriamente em 5 grupos onde implantouse no subcutâneo tubos de butterfly contendo o biomaterial por períodos de preservação recomendados pela norma ADA 10993 para teste de reação tecidual. Os resultados demonstram que o Sistema BoneLithium apresenta reação tecidual normal. Para a avaliação do comportamento biológico do Sistema BoneLithium foram utilizados coelhos brancos adultos da raça New Zealand nos quais defeitos ósseos bilaterais de 1 cm de diâmetro foram confeccionados cirurgicamente na calvária e foram Tratados com o Sistema Bone Lithium do (lado Direito) e somente o Gel de Carbopol (lado esquerdo)/Coágulo sanguíneo (controle). A Histomorfometria demonstrou comportamento favorável ao reparo ósseo e adicionalmente através de Microtomografia Computadorizada (CT SKYSCAN), foi possível constatar diferenças significativas considerando p> 0.05 (ANOVA, Tukey) para o processo de reparo ósseo. A avaliação da performance do Sistema BoneLithium utilizando ratos Wistar nos quais foram criados defeitos críticos no centro da calvária e tratados com diferentes modalidades de enxertos ósseos (controle, autógeno, osso de banco (Unioss®, Marília Brasil), Bio-Oss® e associações com o Sistema BoneLithium. A histomorfometria mostrou diferenças significativas considerando p> 0.05 (ANOVA, Tukey) para avaliação de tecido conjuntivo pré-osteogênico e tecido ósseo neoformado, e quando avaliado qualitativamente por tomografia computadorizada de feixe cônico (I cat Cone Beam FOV 0.05 Xoran Tecnology, LLC, EUA e E-vol, CDT, Brasil), observaram-se áreas de neoformação óssea compatíveis com hiperdensidade óssea em toda a extensão do defeito quando apuradas em analises de paridade em escala Hounsfield. Dessa forma, conclui-se que no contexto deste estudo é possível concluir que Sistema BoneLithium representa uma alternativa com potencial viabilidade clínica e necessita seguimento de aplicação em novas metodologias. / In this work, bases for the development, characterization and evaluation of the biological aspects related to the BoneLithium System were designed, based on the association of lithium carbonate particles dispersed in carbopol® gel matrix, capable of acting as a drug-releasing system. Methodologically this study was divided in four parts: In the first one, the central objective was the development and characterization of the biomaterial through the pharmacological manipulation. In the second step, the tissue reaction was evaluated in subcutaneous of rats, in the third the influence of the lithium particles released by BoneLithium System in the bone repair through experimental models using rabbits, and finally, the capacity of healing of bone defects created surgically in Calvaria of rats, treated with the biomaterial and different options of bone grafts with the objective to compare the efficiency of the BoneLithium System to the preexisting protocols. Experimentally, the tissue reaction was evaluated in which 15 male rats were randomly divided into 5 groups, where butterfly tubes containing the biomaterial were implanted in the subcutaneous tubes for preservation periods recommended by the ADA 10993 standard for biocompatibility test. The results demonstrate that the BoneLithium System is tissue reaction positive. To evaluate the biological behavior of the BoneLithium System, adult New Zealand white rabbits were used in which bilateral bone defects of 1 cm in diameter were surgically made on calvaria and treated with the Bone Lithium System (right side) and only Gel Of Carbopol (left side) / blood clot (control). Histomorphometry showed a favorable behavior to bone repair and, in addition, through Computerized Microtomography (CT SKYSCAN), it was possible to verify significant differences considering p> 0.05 (ANOVA, Tukey) for the bone repair process. The evaluation of the performance of the BoneLithium System using Wistar rats in which critical defects were created at the calvarial center and treated with different bone graft modalities (control, autogenous, bone bank (Unioss®, Marília Brazil), Bio-Oss® and associations (ANOVA, Tukey) for evaluation of pre osteogenic connective tissue and neoformed bone tissue, and when assessed qualitatively by cone beam computed tomography (I cat - Cone Beam - FOV 0.05 - Xoran Tecnology, LLC, USA and E-vol, CDT, Brazil), areas of bone neoformation compatible with bone hyperdensity throughout the extent of the defect were ascertained in Hounsfield scale parity analyzes, It is concluded that in the context of this study it is possible to conclude that the BoneLithium System represents an alternative with potential clinical feasibility And requires follow-up of application in new methodologies.
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A system of the process planning system on the Maintenance of Turbine BladesHuang, Jia-Zi 07 August 2002 (has links)
Abstract
Maintenance makes machine life long more and gets no loss about a chain reaction on part broken. Repair a part that has high complex and is important. It need engineer experience to decide its maintenance process plan. For turbine blade maintenance style that has various type and a small quantity. Different broken feature have different maintenance process in the maintenance process planning stage.
This thesis creates a system for this property maintenance on the process planning system on the Maintenance of turbine blades. Analyzing blade broken feature and classifying the feature to bring up a case-based reasoning and fuzzy set operation that usually see in expert systems. It uses case-based databases to save engineer experiences and analysis to decide critical maintenance process factors. The critical factors can help find out similarity case and modulate a newer case. It needs to create a proto-type system for these purposes. The first, classifying turbine blades broken feature and coding the repair process for different maintenances. The second, using fuzzy set language to determine case¡¦s similarity and finding out algorithm. Integrating product data management system and the system. The research uses a company as case study to test and verify the proto-type system.
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Opportunistic maintenance policy of a multi-unit system under transient stateJain, Sulabh 01 June 2005 (has links)
Most modern systems are equipped with very complex, expensive, and high technology components whose maintenance costs have become an increasingly large portion of the total operating cost of these systems. Thus, the efficacy of the maintenance policy for these and related systems has become a major concern to both manufacturing and design engineers. Different kinds of maintenance strategies have been proposed to solve the problem. While some of these have proven effective, there is yet no definitive approach that has been found that support the maintainability requirements of transient systems or systems that exhibit transient behavior. Transient behavior is the notion of non-steady state operation, which is the characteristic of system operation during its useful life.
For designing convenience most of the maintenance strategies have assumed negligible maintenance or repair time which is not practical.In this research an opportunistic maintenance (OM) approach is implemented on a multi-unit system that exhibits transient behavior. Under OM policy, if a maintenance event has been scheduled for certain components and in the process of implementing the scheduled maintenance of these targeted components, the maintenance of other components whose maintenance times are in close proximity is also implemented at the same time. As a result, the time and cost of marshalling and staging maintenance resources are reduced. As part of the system effectiveness measure, the instantaneous system availability based on the transient nature of the system, is estimated using the renewal theory approach.
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Desenvolvimento, caracterização e desempenho biológico de um novo biomaterial de liberação controlada à base de carbonato de lítio aplicado ao reparo ósseo / Development, characterization and biological performance of a new biomaterial of controlled release based on lithium carbonate applied to bone repairLeonardo Marques 09 June 2017 (has links)
Neste trabalho desenhou-se bases de desenvolvimento, caracterização e avaliação dos aspectos biológicos relacionados ao Sistema BoneLithium, idealizado a partir da associação de partículas de carbonato de lítio dispersas em matriz gel de carbopol®, com capacidade de atuar como um sistema liberador de fármacos. Metodologicamente este estudo se dividiu em quatro partes: Na primeira delas, o objetivo central foi o desenvolvimento e a caracterização do biomaterial através da manipulação farmacológica. Na segunda etapa, avaliou-se a reação tecidual em subcutâneo de ratos, na terceira a influência das partículas de lítio liberadas pelo Sistema BoneLithium no reparo ósseo através de modelos experimentais utilizando coelhos, e por ultimo, a capacidade de cicatrização de defeitos ósseos criados cirurgicamente em calvária de ratos, tratados com o biomaterial e diferentes opções de enxertos ósseos com o objetivo de comparar a eficiência do Sistema BoneLithium aos protocolos pré-existentes. Experimentalmente, avaliou-se a reação tecidual onde se utilizou 15 ratos machos divididos aleatatoriamente em 5 grupos onde implantouse no subcutâneo tubos de butterfly contendo o biomaterial por períodos de preservação recomendados pela norma ADA 10993 para teste de reação tecidual. Os resultados demonstram que o Sistema BoneLithium apresenta reação tecidual normal. Para a avaliação do comportamento biológico do Sistema BoneLithium foram utilizados coelhos brancos adultos da raça New Zealand nos quais defeitos ósseos bilaterais de 1 cm de diâmetro foram confeccionados cirurgicamente na calvária e foram Tratados com o Sistema Bone Lithium do (lado Direito) e somente o Gel de Carbopol (lado esquerdo)/Coágulo sanguíneo (controle). A Histomorfometria demonstrou comportamento favorável ao reparo ósseo e adicionalmente através de Microtomografia Computadorizada (CT SKYSCAN), foi possível constatar diferenças significativas considerando p> 0.05 (ANOVA, Tukey) para o processo de reparo ósseo. A avaliação da performance do Sistema BoneLithium utilizando ratos Wistar nos quais foram criados defeitos críticos no centro da calvária e tratados com diferentes modalidades de enxertos ósseos (controle, autógeno, osso de banco (Unioss®, Marília Brasil), Bio-Oss® e associações com o Sistema BoneLithium. A histomorfometria mostrou diferenças significativas considerando p> 0.05 (ANOVA, Tukey) para avaliação de tecido conjuntivo pré-osteogênico e tecido ósseo neoformado, e quando avaliado qualitativamente por tomografia computadorizada de feixe cônico (I cat Cone Beam FOV 0.05 Xoran Tecnology, LLC, EUA e E-vol, CDT, Brasil), observaram-se áreas de neoformação óssea compatíveis com hiperdensidade óssea em toda a extensão do defeito quando apuradas em analises de paridade em escala Hounsfield. Dessa forma, conclui-se que no contexto deste estudo é possível concluir que Sistema BoneLithium representa uma alternativa com potencial viabilidade clínica e necessita seguimento de aplicação em novas metodologias. / In this work, bases for the development, characterization and evaluation of the biological aspects related to the BoneLithium System were designed, based on the association of lithium carbonate particles dispersed in carbopol® gel matrix, capable of acting as a drug-releasing system. Methodologically this study was divided in four parts: In the first one, the central objective was the development and characterization of the biomaterial through the pharmacological manipulation. In the second step, the tissue reaction was evaluated in subcutaneous of rats, in the third the influence of the lithium particles released by BoneLithium System in the bone repair through experimental models using rabbits, and finally, the capacity of healing of bone defects created surgically in Calvaria of rats, treated with the biomaterial and different options of bone grafts with the objective to compare the efficiency of the BoneLithium System to the preexisting protocols. Experimentally, the tissue reaction was evaluated in which 15 male rats were randomly divided into 5 groups, where butterfly tubes containing the biomaterial were implanted in the subcutaneous tubes for preservation periods recommended by the ADA 10993 standard for biocompatibility test. The results demonstrate that the BoneLithium System is tissue reaction positive. To evaluate the biological behavior of the BoneLithium System, adult New Zealand white rabbits were used in which bilateral bone defects of 1 cm in diameter were surgically made on calvaria and treated with the Bone Lithium System (right side) and only Gel Of Carbopol (left side) / blood clot (control). Histomorphometry showed a favorable behavior to bone repair and, in addition, through Computerized Microtomography (CT SKYSCAN), it was possible to verify significant differences considering p> 0.05 (ANOVA, Tukey) for the bone repair process. The evaluation of the performance of the BoneLithium System using Wistar rats in which critical defects were created at the calvarial center and treated with different bone graft modalities (control, autogenous, bone bank (Unioss®, Marília Brazil), Bio-Oss® and associations (ANOVA, Tukey) for evaluation of pre osteogenic connective tissue and neoformed bone tissue, and when assessed qualitatively by cone beam computed tomography (I cat - Cone Beam - FOV 0.05 - Xoran Tecnology, LLC, USA and E-vol, CDT, Brazil), areas of bone neoformation compatible with bone hyperdensity throughout the extent of the defect were ascertained in Hounsfield scale parity analyzes, It is concluded that in the context of this study it is possible to conclude that the BoneLithium System represents an alternative with potential clinical feasibility And requires follow-up of application in new methodologies.
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Implication des canaux K+ dans les processus de réparation de l’épithélium respiratoire sain et fibrose kystiqueTrinh, Nguyen Thu Ngan 07 1900 (has links)
La pathologie de la fibrose kystique (FK) est causée par des mutations du gène
codant pour le canal Cl- CFTR. Au niveau respiratoire, cette dysfonction du transport
transépithélial de Cl- occasionne une altération de la composition et du volume du liquide de surface des voies aériennes. Une accumulation de mucus déshydraté favorise alors la colonisation bactérienne et une réponse inflammatoire chronique, entraînant des lésions épithéliales sévères au niveau des voies aériennes et des alvéoles pouvant culminer en défaillance respiratoire.
Le principal objectif de mon projet de maîtrise était d’étudier les processus de
réparation de l’épithélium alvéolaire sain, l’épithélium bronchique sain et FK à l’aide
d’un modèle in vitro de plaies mécaniques. Nos résultats démontrent la présence d’une
boucle autocrine EGF/EGFR contrôlant les processus de migration cellulaire et de
réparation des lésions mécaniques. D’autre part, nos expériences montrent que l’EGF
stimule l’activité et l’expression des canaux K+ KATP, KvLQT1 et KCa3.1 des cellules
épithéliales respiratoires. L’activation de ces canaux est cruciale pour les processus de
réparation puisque la majeure partie de la réparation stimulée à l’EGF est abolie en
présence d’inhibiteurs de ces canaux. Nous avons également observé que les cellules FK
présentent un délai de réparation, probablement causé par un défaut de la réponse EGF/EGFR et une activité/expression réduite des canaux K+.
Nos résultats permettent de mieux comprendre les mécanismes de régulation des processus de réparation de l’épithélium sain et FK. De plus, ils ouvrent de nouvelles options thérapeutiques visant à promouvoir, à l’aide d’activateurs de canaux K+ et de facteurs de croissance, la régénération de l’épithélium respiratoire chez les patients atteints de FK. / The cystic fibrosis pathology (CF) is caused by mutations of the gene coding for
the Cl- channel, CFTR. In the lungs, the dysfunction of transepithelial ion transport leads
to an alteration of airway surface liquid volume and composition. Dehydrated mucus
accumulation then favors chronic bacterial colonisation and inflammatory response,
inducing severe epithelial injuries in the airways and the alveoli and then, respiratory
failure.
The main objective of my master degree project was to study normal alveolar,
normal and CF bronchial epithelia repair processes using an in vitro model of mechanical wound-healing. Our results reveal the presence of an EGF/EGFR autocrine loop that controls cell migration and wound-healing. Our results show also that EGF signaling stimulate KATP, KvLQT1 and KCa3.1 K+ channel activity and expression in epithelial cells. K+ channel activation is crucial for repair processes since K+ channel inhibitors prevent most of EGF-stimulated wound-healing. We also observed that CF cells present delayed repair processes, probably caused by an EGF signaling defect and reduced K+ channel activity and expression.
Our results allow us to better understand the regulatory mechanisms of normal
and CF epithelial repair processes. Futhermore, our results open to new therapeutic options that promote, with K+ channel activators and growth factors, respiratory epithelium regeneration in CF patients.
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Implication des canaux K+ dans les processus de réparation de l’épithélium respiratoire sain et fibrose kystiqueTrinh, Nguyen Thu Ngan 07 1900 (has links)
La pathologie de la fibrose kystique (FK) est causée par des mutations du gène
codant pour le canal Cl- CFTR. Au niveau respiratoire, cette dysfonction du transport
transépithélial de Cl- occasionne une altération de la composition et du volume du liquide de surface des voies aériennes. Une accumulation de mucus déshydraté favorise alors la colonisation bactérienne et une réponse inflammatoire chronique, entraînant des lésions épithéliales sévères au niveau des voies aériennes et des alvéoles pouvant culminer en défaillance respiratoire.
Le principal objectif de mon projet de maîtrise était d’étudier les processus de
réparation de l’épithélium alvéolaire sain, l’épithélium bronchique sain et FK à l’aide
d’un modèle in vitro de plaies mécaniques. Nos résultats démontrent la présence d’une
boucle autocrine EGF/EGFR contrôlant les processus de migration cellulaire et de
réparation des lésions mécaniques. D’autre part, nos expériences montrent que l’EGF
stimule l’activité et l’expression des canaux K+ KATP, KvLQT1 et KCa3.1 des cellules
épithéliales respiratoires. L’activation de ces canaux est cruciale pour les processus de
réparation puisque la majeure partie de la réparation stimulée à l’EGF est abolie en
présence d’inhibiteurs de ces canaux. Nous avons également observé que les cellules FK
présentent un délai de réparation, probablement causé par un défaut de la réponse EGF/EGFR et une activité/expression réduite des canaux K+.
Nos résultats permettent de mieux comprendre les mécanismes de régulation des processus de réparation de l’épithélium sain et FK. De plus, ils ouvrent de nouvelles options thérapeutiques visant à promouvoir, à l’aide d’activateurs de canaux K+ et de facteurs de croissance, la régénération de l’épithélium respiratoire chez les patients atteints de FK. / The cystic fibrosis pathology (CF) is caused by mutations of the gene coding for
the Cl- channel, CFTR. In the lungs, the dysfunction of transepithelial ion transport leads
to an alteration of airway surface liquid volume and composition. Dehydrated mucus
accumulation then favors chronic bacterial colonisation and inflammatory response,
inducing severe epithelial injuries in the airways and the alveoli and then, respiratory
failure.
The main objective of my master degree project was to study normal alveolar,
normal and CF bronchial epithelia repair processes using an in vitro model of mechanical wound-healing. Our results reveal the presence of an EGF/EGFR autocrine loop that controls cell migration and wound-healing. Our results show also that EGF signaling stimulate KATP, KvLQT1 and KCa3.1 K+ channel activity and expression in epithelial cells. K+ channel activation is crucial for repair processes since K+ channel inhibitors prevent most of EGF-stimulated wound-healing. We also observed that CF cells present delayed repair processes, probably caused by an EGF signaling defect and reduced K+ channel activity and expression.
Our results allow us to better understand the regulatory mechanisms of normal
and CF epithelial repair processes. Futhermore, our results open to new therapeutic options that promote, with K+ channel activators and growth factors, respiratory epithelium regeneration in CF patients.
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Reaction Paths of Repair Fragments on Damaged Ultra-low-k SurfacesFörster, Anja 16 February 2015 (has links) (PDF)
In the present work, the plasma repair for damaged ultra-low-k (ULK) materials, newly developed at the Fraunhofer ENAS, is studied with density functional theory (DFT) and molecular dynamic (MD) methods to obtain new insights into this repair mechanism. The ULK materials owe their low dielectric constant (k-value) to the insertion of k-value lowering methyl groups. During the manufacturing process, the ULK materials are damaged and their k-values increase due to the adsorbtion of hydroxyl groups (OH-damage) and hydrogen atoms (H-damage) that replaced themethyl groups.
The first investigation point is the creation of repair fragments. For this purpose the silylation molecules bis(dimethylamino)-dimethylsilane (DMADMS) and octamethylcyclotetrasiloxane (OMCTS) are fragmented. Here, only fragmentation reactions that lead to repair fragments that contain one silicon atom and at least one methyl group were considered. It is shown that the repair fragments that contain three methyl groups are preferred, especially in a methyl rich atmosphere.
The effectivity of the obtained repair fragments to cure an OH- and H-damage are investigated with two model systems. The first system consists of an assortment of small ULK-fragments, which is used to scan through the wide array of possible repair reactions. The second system is a silicon oxide cluster that investigates whether the presence of a cluster influences the reaction energies.
In both model systems, repair fragments that contain three methyl groups or two oxygen atoms are found to be most effective. Finally, the quantum chemical results are compared to experimental findings to get deeper insight into the repair process.
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Efecto del plasma rico en plaquetas en la incorporación biológica de una plastia tendinosa en un túnel óseoRuiz Macarrilla, Leonardo 25 February 2011 (has links)
OBJETIVO
El objetivo nuestro proyecto de investigación es demostrar si la presencia de plasma rico en plaquetas (PRP) autólogo, que aporta varios factores de crecimiento en proporciones fisiológicas, en la interfase injerto tendinoso-hueso modifica el proceso de reparación de los tejidos locales en nuestro modelo experimental.
MATERIAL Y MÉTODO
Diseñamos un estudio de tipo experimental, longitudinal y prospectivo.
Diez conejos albinos de Nueva Zelanda son intervenidos en sus 2 extremidades traseras, en una se realiza la técnica Experimental aplicando PRP y la otra sirve de control, sin aplicar PRP . Una vez intervenidos, los animales son divididos en 2 grupos de 5 de forma aleatoria. Un grupo es sacrificado a la 2ª semana y el otro a la 4ª semana postintervención.
El área quirúrgica fue estudiada macroscópica, radiográfica, histológica e inmunohistoquímicamente.
RESULTADOS
El estudio macroscópico y radiográfico de las piezas muestra la presencia de un área de reacción ósea de mayor tamaño en la zona de entrada del tendón en el interior del túnel óseo en el grupo Experimental sacrificado a la 4ª sem. respecto al resto de grupos.
Microscópicamente, en ningún corte histológico, tanto de los grupos Control como Experimental, se observa la línea de separación basófila, denominada tidemark190 entre el fibrocartílago mineralizado y el no mineralizado. Si se evidenció la aparición de una mayor cantidad de los elementos tisulares fibrosos, cartilaginosos y osteoides, y un mayor grado de ordenación celular en el fibrocartílago y sustancia osteoide en los grupos Experimentales respecto a sus correspondientes grupos Control. Además se observa la aparición de zonas de anclaje hueso-tendón en dos extremidades del grupo Experimental sacrificado a la 4ª sem., no encontradas en ningún caso Control.
En el estudio inmunohistoquímico no apreciamos diferencias en el patrón de distribución del colágeno tipo II entre los distintos grupos, aunque si existe una mayor área de tinción de colágeno tipo II en los grupos Experimentales respecto a sus correspondientes grupos Control, ya que la cantidad de tejido fibrocartilaginoso, en sus variadas formas de presentación, también es mayor en los grupos que recibieron PRP.
CONCLUSIONES
El análisis de los resultados concluye que macroscópica, radiográfica y microscópicamente, este estudio demuestra que la administración de PRP como fuente de FC afecta al proceso de reparación del autoinjerto tendinoso dentro de un túnel óseo en nuestro modelo experimental. El PRP aceleró la curación, anticipando en el tiempo la aparición de elementos celulares y tisulares propios de fases posteriores del proceso de cicatrización, y además incrementó la cantidad éstos. / EFFECT OF PLATELET RICH PLASMA IN THE BIOLOGIC INCORPORATION OF A TENDON GRAFT IN A BONE TUNNEL
Objective: To study the effect of the platelet rich plasma (PRP) in the biological process of tendon healing inside a bone tunnel
Material y Methods:
We designed an experimental, longitudinal and prospective research study. Ten animals were operated on their back legs (one as Experimental group and the other as Control group). In the Experimental group the tendon of the internal gastrocnemius muscle was cut, infiltrated with PRP, placed in a tibial bone tunnel filled with PRP, and finally, fixed by a bioabsorbable screw. The same procedure was practiced in the Control group without the use of PRP. Five rabbits were sacrificed on the second week and five on the fourth week after surgery.
The legs were evaluated macroscopic, radiographic, histologic and immunohistochemically.
Results: The macroscopic and radiographic study of the pieces shows the presence of a larger bone reaction area in the entrance of the tendon within the bone tunnel in the Experimental group sacrificed on the fourth week compared to other groups. Microscopically, the "tidemark" isn’t observed between mineralized and unmineralized fibrocartilage in any group . The Experimental group presents a statistically significant greater amount of chondroid tissue, osteoid tissue and trabecular bone, both the second and fourth week. We observe continuity zones between tendinous fibers and bone in some cases of the Experimental group on the fourth week . Immunohistochemically, no appreciable differences in the distribution pattern of type II collagen between the groups could be seen, although there is a greater area of staining in the Experimental group compared to their corresponding Control group
Conclusion:
The analysis of results and assessment of the comparison established between the research groups concludes that macroscopic, radiographic and microscopically, this study demonstrates that administration of PRP affects the repair process of tendon autograft in a bone tunnel in our experimental model. The PRP accelerated the healing time anticipating the emergence of cellular and tissue elements characteristic of later stages of the healing process, and also increased the amount of them.
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Reaction Paths of Repair Fragments on Damaged Ultra-low-k SurfacesFörster, Anja 25 September 2014 (has links)
In the present work, the plasma repair for damaged ultra-low-k (ULK) materials, newly developed at the Fraunhofer ENAS, is studied with density functional theory (DFT) and molecular dynamic (MD) methods to obtain new insights into this repair mechanism. The ULK materials owe their low dielectric constant (k-value) to the insertion of k-value lowering methyl groups. During the manufacturing process, the ULK materials are damaged and their k-values increase due to the adsorbtion of hydroxyl groups (OH-damage) and hydrogen atoms (H-damage) that replaced themethyl groups.
The first investigation point is the creation of repair fragments. For this purpose the silylation molecules bis(dimethylamino)-dimethylsilane (DMADMS) and octamethylcyclotetrasiloxane (OMCTS) are fragmented. Here, only fragmentation reactions that lead to repair fragments that contain one silicon atom and at least one methyl group were considered. It is shown that the repair fragments that contain three methyl groups are preferred, especially in a methyl rich atmosphere.
The effectivity of the obtained repair fragments to cure an OH- and H-damage are investigated with two model systems. The first system consists of an assortment of small ULK-fragments, which is used to scan through the wide array of possible repair reactions. The second system is a silicon oxide cluster that investigates whether the presence of a cluster influences the reaction energies.
In both model systems, repair fragments that contain three methyl groups or two oxygen atoms are found to be most effective. Finally, the quantum chemical results are compared to experimental findings to get deeper insight into the repair process.:1. Introduction
2. Theoretical Background
2.1. Ultra-low-k Materials
2.1.1. Definition, Usage and Challenges
2.1.2. k-Restore
2.2. Reaction Theory
2.2.1. Reaction Process
2.2.2. Thermal Influence
3. Computational Methods
3.1. Overview
3.2. Density Functional Theory
3.2.1. Theoretical Background
3.2.1.1. The Schrödinger Equation and the Variational Principle
3.2.1.2. From the Electron Density to the Kohn-Sham Approach
3.2.1.3. Exchange-Correlation Functionals and Basis Sets
3.2.2. Used Program Packages
3.3. ReaxFF
3.3.1. Theoretical Background
3.3.2. Used Program Packages
4. Model System
4.1. Damaged ULK Materials
4.1.1. ULK-Fragments
4.1.2. Silicon Oxide Cluster
4.2. Repair Fragments
4.2.1. Overview
4.2.2. Fragmentation of DMADMS
4.2.3. Fragmentation of OMCTS
4.2.4. Continuing Reactions
5. Results and Discussion
5.1. Reactions between Repair Fragments and ULK-Fragments
5.1.1. Repair of OH-damages
5.1.2. Repair of H-damages
5.1.3. Selected Repair Reactions with Gaussian
5.2. Reactions Between Repair Fragments and Silicon Oxide Cluster
5.2.1. Comparison Between ULK-Fragments and Silicon Oxide Cluster
5.2.2. Comparability of DFT and MD Results
5.3. Comparison with Experimental Results
6. Summary and Outlook
A. Appendix
A.1. Temperature Influence .
A.1.1. Temperature Influence on the DMADMS Fragmentation in Dmol3
A.1.2. Temperature Influence on the OMCTS Fragmentation in Dmol3 .
A.2. Tests
A.2.1. DMADMS Fragmentation with Gaussian
A.2.2. G2 Test Set
A.2.3. Calculation Time of the Silicon Oxide Cluster in Dmol3
A.3. Error Analysis
A.3.1. Basis Set Superposition Error in Dmol3
A.3.2. Dispersion Correction
A.4. Illustration of Defects
A.5. Bookmark
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Energetic and Microscopic Characterization of the Primary Electron Transfer Reaction in the (6-4) Photolyase Repair ReactionOßwald, Mara 17 April 2024 (has links)
Wird DNA mit UV-Licht bestrahlt, kommt es zur Bildung von Photoschäden, die zu Zelltod oder Krebs führen können. In dieser Arbeit wird die primäre Elektronentransferreaktion des lichtaktivierten Reparaturprozesses des (6-4)-Schadens in Drosophila melanogaster charakterisiert. Der katalytische Reparaturzyklus wird durch das Flavoprotein (6-4)-Photolyase (PL) realisiert. Der Elektronentransfer (ET) vom Flavin-Adenin-Dinukleotid (FADH⁻) Kofaktor zum Schaden initiiert die molekularen Umlagerungen. Diese Arbeit charakterisiert die primäre ET Reaktion mithilfe von molekulardynamischen Langzeitsimulationen (µs) in Kombination mit Quantenmechanik/Molekularmechanik-Simulationen. Ab initio lokale Coupled-Cluster- und Dichtefunktionaltheorierechnungen wurden angewendet, um die relative Energetik von lokal angeregten und Ladungstransferzuständen des (6-4)-Reparaturkomplexes zu charakterisieren. Es zeigt sich, dass die Reduktion des (6-4)-Schadens durch einen Ladungstransferzustand ermöglicht wird an dem die Adeninstruktur des FADH⁻ -Kofaktors beteiligt ist. Über die Simulationen wird ein mikroskopisches Bild der Reaktionskoordinate der Elektronentransferreaktion im Marcusbild entwickelt. Diese ist nicht vollständig durch parabolische freie Energiekurven beschrieben sondern wird, durch Wechselwirkungen in der aktiven Tasche, ein Multiminima-Reaktionspfad ausgebildet. Hierbei hat die Rotation der Seitenkette der benachbarten, geladenen Aminosäure Lys246 dominanten Einfluss. Dies legt nahe, dass die primäre ET Reaktion der (6-4) Schadensreparatur, einen vom Adenin unterstützten ET Weg von der PL zur 5’ Seite des Schadens nimmt. Dieser Prozess wird durch benachbarte Aminosäuren und einer Stärkung der Wasserstoffbrücken mit Wassermolekülen stabilisiert. Die Ergebnisse dieser Arbeit zeigen, dass ET-Reaktionen in komplexen enzymatischen Systemen nicht im Kontinuumsbild von ET beschrieben werden können, da lokale Wechselwirkungen drastischen Einfluss auf die ET Reaktionen haben. / UV-light irradiation of DNA leads to the formation of photolesions that can cause cell death and cancer. This thesis aims at the characterization of the primary electron transfer (ET) reaction in the photoactivated repair process of the (6-4) lesion in Drosophila melanogaster. The catalytic repair cycle is realized by a flavoprotein called photolyase (PL). The ET from the fully reduced flavin-adenine-dinucleotide (FADH⁻) cofactor of the PL to the lesion initiates molecular rearrangements. In this thesis fluctuation properties of the enzyme environment on the excited states are considered by conducting long-time (µs) molecular dynamics simulations combined with extensive quantum mechanical/molecular mechanical simulations. Ab initio local coupled cluster simulations and density functional theory are applied to characterize the relative energetics of locally excited and charge transfer (CT) states in the (6-4) lesion repair complex. Reduction of the (6-4) lesion is found to be enabled by a CT state involving the adenine moiety of the FADH⁻ cofactor. Microscopic characterization of a Marcus-type free energy reaction coordinate reveals that it cannot be fully described by parabolic free energy curves. Specifically, rotation of the side chain of nearby charged amino acid Lys246 imposes a double-well character on the potential energy surface along the reaction coordinate of the ET. For the ET reaction triggering the catalytic (6-4) lesion repair, the findings of this thesis suggest an ET pathway to the 5’ side of the (6-4) lesion mediated by the adenine moiety. The process is stabilized by neighboring amino acids and a strengthening of hydrogen bonds with water molecules. The presented results demonstrate that ET reactions in complex enzymatic systems cannot be described within the continuum ET picture, as local interactions drastically tune the ET reaction.
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